Why is a clean template for your Sanger sequencing reaction important? And what are the ways to get it without losing too much precious samples?\u00a0\u00a0 Let\u2019s find out today.<\/p>\n
The sequencing workflow usually starts with PCR also known as Polymerase Chain Reaction, where the target fragment is replicated into thousands of copies under a controlled condition using primers, DNA polymerases and deoxynucleotides. Before the PCR product is ready for Sanger sequencing, you must do one more thing, a PCR clean up step. Because Sanger sequencing is a highly accurate technique for you to read DNA sequence base by base, it is very important to clean up your reaction mixtures so that those unincorporated primers and dNTPs won\u2019t interfere with your results.<\/p>\n
So how do you perform this PCR clean up step and what are your options?<\/p>\n