{"id":7872,"date":"2016-03-08T12:31:40","date_gmt":"2016-03-08T17:31:40","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=7872"},"modified":"2025-03-12T19:08:45","modified_gmt":"2025-03-12T19:08:45","slug":"introducing-the-crispr-genome-editing-playlist","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/introducing-the-crispr-genome-editing-playlist\/","title":{"rendered":"Introducing The CRISPR Genome Editing Playlist"},"content":{"rendered":"<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/E9QkhgstKeo?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p style=\"text-align: left\"><a href=\"https:\/\/www.youtube.com\/playlist?list=PLGlvFEwL2wDHcUWw5oTabKACDN5C2gH7r\" target=\"_blank\">CRISPR Genome Editing Playlist<\/a><\/p>\n<p><strong>What is this CRISPR craze all about?<\/strong><\/p>\n<p style=\"text-align: left\">If you\u2019ve been paying attention to the news, CRISPR is a topic on everyone\u2019s lips; from scientific journals to mainstream television coverage and even to social forums. So, why is everyone talking about CRISPR? In order to better understand that, we need to take a look at where we\u2019ve come from so that it\u2019s possible to see why CRISPR technology is such a breakthrough.<\/p>\n<p><strong>Background:<\/strong><\/p>\n<p>Genetic engineering has had \u201cmolecular scissors\u201d since the 1970 discovery of restriction endonucleases. While studying bacteria and their resistance to viral infections, scientists were cued into a built-in self-defense within bacterial cells called restriction enzymes, also known as restriction endonucleases. These enzymes possessed the ability to destroy invading viral DNA by cleaving it, thereby preserving the bacteria. It was this ability to \u201ccut DNA\u201d that opened doors to gene editing. \u00a0Since that period, families of restriction enzymes have been developed as the bourgeoning field of genetic engineering advanced. Today, Meganucleases, Zinc finger nucleases, <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-tals.html\" target=\"_blank\">TALEN<\/a> and now <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-crispr.html\" target=\"_blank\">CRISPR<\/a> are the four types of engineered restriction enzymes that dominate the scene. Each one harnesses the molecular technology that makes gene manipulation possible, from deletion and insertion to activation and knock-outs. This has impacted various fields of scientific research, leading to advancements in areas such as cancer therapies, genomics (The Human Genome Project) and agricultural biology.<\/p>\n<div id=\"attachment_7873\" style=\"width: 310px\" class=\"wp-caption alignright\"><a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-crispr\/crispr-cas9-based-genome-editing.html\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-7873\" class=\"size-medium wp-image-7873\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-1-300x241.jpg\" alt=\"CRISPR-Cas9 system gene editing tool\" width=\"300\" height=\"241\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-1-300x241.jpg 300w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-1-768x617.jpg 768w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-1-1024x822.jpg 1024w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-1-400x321.jpg 400w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-1.jpg 1106w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-7873\" class=\"wp-caption-text\">The CRISPR-Cas9 system gene editing tool consists of the Cas9 nuclease and guide RNA made up of target-complementary crRNA and an auxiliary tracrRNA.<\/p><\/div>\n<p>With the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-crispr\/crispr-cas9-based-genome-editing.html\">CRISPR-Cas9 system<\/a> being the most recent pair of \u201cmolecular scissors\u201d to add to the research tool repertoire, the endless possibilities of genetic engineering become much more tangible. CRISPR (Clustered Regularly Interspaced Palindromic Repeats) and Cas9 (CRISPR Associated Proteins) endonuclease are part of a bacterial adaptive immune system that benefits from viral DNA stored from previous bacteriophage attacks. This stored DNA acts as a reminder of past invaders and once transcribed, the resulting snippets of complementary RNA guide the Cas9 protein to invading bacteriophage DNA to cleave its genetic material, ceasing the takeover. In a research setting, scientists can design and create gRNA (guide RNA) which can then lead the Cas9 enzyme to make a \u201ccut\u201d at the appropriate DNA locus. Therefore, virtually any gene being studied can be easily targeted by the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-crispr\/crispr-cas9-based-genome-editing.html\">CRISPR-Cas9 system<\/a>. Access to this flexible technology delivers the most efficient and simplest method of gene editing to date.<\/p>\n<p>Pest-resistant lettuce, the elimination of diseases such as cystic fibrosis or anemia \u2013are some choice research topics CRISPR Pioneer Jennifer Doudna shared with <em>Nature<\/em> when describing how her fellow colleagues are using the technology she helped discover.<\/p>\n<p>\u201cI am excited about the potential for genome engineering to have a positive impact on human life, and on our basic understanding of biological systems,\u201d she expressed.<\/p>\n<p>We couldn\u2019t agree more. Craving more on CRISPR? Check out our <a href=\"https:\/\/www.youtube.com\/playlist?list=PLGlvFEwL2wDHcUWw5oTabKACDN5C2gH7r\">CRISPR Genome Editing Playlist<\/a>.<\/p>\n<p><a href=\"https:\/\/www.youtube.com\/watch?v=pymz0ftGG5k&amp;index=2&amp;list=PLGlvFEwL2wDHcUWw5oTabKACDN5C2gH7r\">Gene Engineering eBook: Genome Editing and Gene Modulation<\/a><\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-crispr.html\">GeneArt CRISPR \u2013 Simple Genome Editing Starts Here<\/a><\/p>\n<div id=\"attachment_7874\" style=\"width: 310px\" class=\"wp-caption alignleft\"><a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-crispr\/crispr-protein.html\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-7874\" class=\"wp-image-7874 size-medium\" src=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-2.jpg-300x220.png\" alt=\"CRISPR-Cas9 System THERMO 2.jpg\" width=\"300\" height=\"220\" srcset=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-2.jpg-300x220.png 300w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-2.jpg-768x563.png 768w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-2.jpg-400x293.png 400w, https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-Cas9-System-THERMO-2.jpg.png 946w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/a><p id=\"caption-attachment-7874\" class=\"wp-caption-text\">ThermoFisher Scientific offers CRISPR-Cas9 system gene editing tools in four formats: an all-in-one expression vector; Cas9 mRNA; Cas9 protein and CRISPR Libraries services paired with optimal reagents and delivery methods.<\/p><\/div>\n<p>For Research Use Only. Not for use in diagnostic procedures.<\/p>\n<p>Sources:<\/p>\n<p><a href=\"http:\/\/www.nature.com\/news\/genome-editing-revolution-my-whirlwind-year-with-crispr-1.19063\">http:\/\/www.nature.com\/news\/genome-editing-revolution-my-whirlwind-year-with-crispr-1.19063<\/a><\/p>\n<p><a href=\"http:\/\/www.nature.com\/scitable\/spotlight\/restriction-enzymes-18458113\">http:\/\/www.nature.com\/scitable\/spotlight\/restriction-enzymes-18458113<\/a><\/p>\n<p><a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-crispr\/crispr-cas9-based-genome-editing.html\">http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/genome-editing\/geneart-crispr\/crispr-cas9-based-genome-editing.html<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>CRISPR Genome Editing Playlist What is this CRISPR craze all about? If you\u2019ve been paying attention to the news, CRISPR is a topic on everyone\u2019s lips; from scientific journals to mainstream television coverage and even to social forums. So, why is everyone talking about CRISPR? In order to better understand that, we need to take<\/p>\n","protected":false},"author":125,"featured_media":8411,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[2452,101],"tags":[126,127,104,115],"division":[],"class_list":{"0":"post-7872","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-gene-editing","8":"category-product-news","9":"tag-crispr-cas9","10":"tag-gene-editing","11":"tag-gene-expression","12":"tag-product-video","13":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Introducing The CRISPR Genome Editing Playlist - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/introducing-the-crispr-genome-editing-playlist\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Introducing The CRISPR Genome Editing Playlist\" \/>\n<meta property=\"og:description\" content=\"CRISPR Genome Editing Playlist What is this CRISPR craze all about? If you\u2019ve been paying attention to the news, CRISPR is a topic on everyone\u2019s lips; from scientific journals to mainstream television coverage and even to social forums. So, why is everyone talking about CRISPR? In order to better understand that, we need to take\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/introducing-the-crispr-genome-editing-playlist\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2016-03-08T17:31:40+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2025-03-12T19:08:45+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/03\/CRISPR-CAS9-gene-editing-complex-from-Streptococcus-pyogenes.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1000\" \/>\n\t<meta property=\"og:image:height\" content=\"859\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Grace Yco\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Grace Yco\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/introducing-the-crispr-genome-editing-playlist\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/introducing-the-crispr-genome-editing-playlist\\\/\"},\"author\":{\"name\":\"Grace Yco\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/dccad2f709bc0af3d63bbed6a240122f\"},\"headline\":\"Introducing The CRISPR Genome Editing Playlist\",\"datePublished\":\"2016-03-08T17:31:40+00:00\",\"dateModified\":\"2025-03-12T19:08:45+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/introducing-the-crispr-genome-editing-playlist\\\/\"},\"wordCount\":602,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/introducing-the-crispr-genome-editing-playlist\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2016\\\/03\\\/CRISPR-CAS9-gene-editing-complex-from-Streptococcus-pyogenes.jpg\",\"keywords\":[\"CRISPR-Cas9\",\"Gene Editing\",\"gene expression\",\"Product Video\"],\"articleSection\":[\"Gene Editing\",\"Product News\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/introducing-the-crispr-genome-editing-playlist\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/introducing-the-crispr-genome-editing-playlist\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/introducing-the-crispr-genome-editing-playlist\\\/\",\"name\":\"Introducing The CRISPR Genome Editing Playlist - 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