{"id":8197,"date":"2016-04-04T18:28:53","date_gmt":"2016-04-04T22:28:53","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=8197"},"modified":"2022-08-18T20:29:12","modified_gmt":"2022-08-18T20:29:12","slug":"hands-on-with-digital-pcr","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/hands-on-with-digital-pcr\/","title":{"rendered":"Hands-on With Digital PCR"},"content":{"rendered":"

Digital PCR<\/a>\u00a0is a relatively new approach to nucleic acid detection and quantification that offers an alternate method to conventional real-time quantitative PCR for absolute quantification and rare allele detection. It all works by partitioning a sample of DNA or cDNA into many individual, parallel PCR reactions; some of these reactions contain the target molecule (positive) while others do not (negative). A single molecule can be amplified a million-fold or more. During amplification,\u00a0TaqMan chemistry<\/a>\u00a0with dye-labeled probes is used to detect sequence-specific targets. When no target sequence is present, no signal is emitted. Following PCR analysis, the fraction of negative reactions is used to generate an absolute count of the number of target molecules in the sample, without the need for standards or endogenous controls.<\/p>\n