{"id":8255,"date":"2016-04-06T15:18:58","date_gmt":"2016-04-06T19:18:58","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=8255"},"modified":"2020-05-28T19:14:03","modified_gmt":"2020-05-28T19:14:03","slug":"all-your-digital-pcr-needs","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/all-your-digital-pcr-needs\/","title":{"rendered":"Guide to Digital PCR – From Education to Workflow"},"content":{"rendered":"

We’ve been sharing with you all things digital PCR<\/a>. From our six-part educational series, which focuses on various aspects of digital PCR to our most recent hands-on complete digital PCR workflow. With these resources handy, we hope to equip you with the tools needed to understand digital PCR, how you can use it for various applications, and a step by step video to guide you through your experiment.<\/p>\n

Go Digital PCR: Pt 1 \u2013 Next Generation Quantification<\/a>\u00a0\"image1\"<\/a><\/strong>
\nWe begin our six-part series introducing you to digital PCR (dPCR), what makes it next generation technology, and how it compares to Real-Time Quantitative PCR<\/a> (qPCR) and traditional PCR<\/a>.<\/p>\n

Go Digital PCR: Pt 2 \u2013 QuantStudio\u2122 3D<\/a><\/strong>
\nIn this edition, we\u2019ll take a look at how the\u00a0Quant<\/a>\"dpcr2\"<\/a><\/strong>Studio\u2122 3D Digital PCR system\u00a0offers simplicity, affordability, and most importantly, absolute quantification attainability.<\/p>\n

Go Digital PCR: Pt 3 \u2013 Copy Number Variation<\/strong><\/a>
\n<\/em>Part 3\u00a0explores how dPCR is enabling researchers\u2019 abilities to achieve precise copy number variation (CNV) analysis<\/a>,\u00a0helping\u00a0detect and quantify small percent copy number differences with a high degree of precision.<\/p>\n

Go Digital PCR: Pt 4 \u2013 NGS Libraries\u00a0<\/a><\/strong>\"4\"<\/a>
\nIn the fourth segment we\u00a0share with you how dPCR enables highly precise next-generation sequencing<\/a> (NGS) library quantification.<\/p>\n

Go Digital PCR: Pt 5 \u2013 Rare Mutations<\/strong>
\n<\/a>In our\u00a0fifth segment we share with you how dPCR is capable of detecting and quantifying rare mutations for low-prevalence targets in cancer research samples.
\n
\nGo Digital PCR: Pt 6 \u2013 Emerging Analyses\u00a0<\/strong>
\n<\/a>This is the final segment in our six-part series introducing you to dPCR and how it aids absolute quantitation and rare sequence detection in lesser used applications.<\/p>\n

\"Digital<\/a>Hands-on With Digital PCR<\/a>\u00a0\u2013<\/a>\u00a0QuantStudio 3D Digital PCR Workflow Video<\/a><\/strong>
\nThe workflow for the\u00a0QuantStudio 3D digital PCR\u00a0system\u00a0was\u00a0recently updated and\u00a0posted on Thermo Fisher Scientific\u2019s YouTube channel<\/a>. Take a look at the workflow in the video below, and let us know if you have any questions in the comments section.<\/p>\n

We hope you’ve enjoyed our educational series and our workflow video. If you have any questions or would like to learn more about a specific topic, please be sure to let us know in the comments section below.<\/p>\n","protected":false},"excerpt":{"rendered":"

We’ve been sharing with you all things digital PCR. From our six-part educational series, which focuses on various aspects of digital PCR to our most recent hands-on complete digital PCR workflow. With these resources handy, we hope to equip you with the tools needed to understand digital PCR, how you can use it for various<\/p>\n","protected":false},"author":120,"featured_media":8259,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[108,101],"tags":[17,57,43],"division":[],"class_list":{"0":"post-8255","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-lab-tips-and-tricks","8":"category-product-news","9":"tag-applied-biosystems","10":"tag-digital-pcr","11":"tag-pcr","12":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nGuide to Digital PCR - From Education to Workflow - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/all-your-digital-pcr-needs\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Guide to Digital PCR - From Education to Workflow\" \/>\n<meta property=\"og:description\" content=\"We’ve been sharing with you all things digital PCR. 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