{"id":8687,"date":"2016-06-21T22:19:42","date_gmt":"2016-06-21T22:19:42","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/behindthebench\/?p=8687"},"modified":"2020-05-28T19:12:50","modified_gmt":"2020-05-28T19:12:50","slug":"5-things-to-help-start-your-ngs-experiments","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/5-things-to-help-start-your-ngs-experiments\/","title":{"rendered":"5 Things to Help Start Your NGS Experiments"},"content":{"rendered":"<p>With the advent of <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/sequencing\/next-generation-sequencing.html?cid=social_btb_targetseq\" target=\"_blank\" rel=\"noopener noreferrer\">Next-Generation Sequencing<\/a> (NGS), more and more researchers are turning to sequencing\u00a0to better\u00a0understand genetics and gene expression. It often seems like a daunting task to trust your precious rare samples for analysis on an instrument you\u00a0don&#8217;t have much experience using.<\/p>\n<p>Our <a href=\"https:\/\/www.youtube.com\/playlist?list=PLGlvFEwL2wDFVLBA1egJFwHyetpVDPExK\" target=\"_blank\" rel=\"noopener noreferrer\">SeqitOut video series<\/a> tackles all things sequencing (from NGS to Sanger).\u00a0To help you get started on your NGS experiments we&#8217;ve put together five tools\u00a0to help you get started with your NGS experiments.\u00a0And if a question pops into your head while you\u2019re watching the videos, just send them in at <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/global\/forms\/seq-it-out-question-submission.html?socid=social_btb\" target=\"_blank\" rel=\"noopener noreferrer\">thermofisher.com\/ask<\/a> \u2013 the team at Thermo Fisher Scientific just might make an episode out of it!<\/p>\n<p>Let&#8217;s get started&#8230;<\/p>\n<h4><a href=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/next-gen-sequencing-acronyms-and-terms-seq-it-out-15\/\" target=\"_blank\" rel=\"noopener noreferrer\">What do all these Next-Generation Sequencing terms mean?<\/a><\/h4>\n<p>We all started with our ABC\u2019s and somehow we ended up falling in love with AGTC\u2019s. But when we step into NGS\u2026.OMG. Most of you already know the acronyms DNA and RNA. But, in the NGS\u00a0world, there is a whole lot more. When you are starting to think about your next sequencing experiment, people may ask if you are doing WES, WGS, or target-seq? Let\u2019s dive into the genetic dictionary that is Next-generation sequencing terminology.<\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/wNzFMV0Zhqw?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<h4><a href=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/how-many-samples-can-i-multiplex-on-my-ngs-run-seq-it-out-13\/\" target=\"_blank\" rel=\"noopener noreferrer\">How many samples can I multiplex on my NGS run?<\/a><\/h4>\n<p>The unprecedented high throughput of NGS instruments is helping drive down the cost of sequencing, making it accessible to large commercial and small research labs, alike. One way to take full advantage of high throughput NGS is sample multiplexing.\u00a0 Multiplex sequencing allows users to sequence more than one sample on the same sequencing run, reducing your cost per sample.\u00a0 Well, this sounds great. But, you may be asking yourself, how does it work?\u00a0 How many samples can I fit on my sequencing run?<\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/3SEz-i517Oo?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<h4><\/h4>\n<h4><a href=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/next-generation-sequencing-library-preparation-seq-it-out-10\/\" target=\"_blank\" rel=\"noopener noreferrer\">What do I need to know about library preparation methods for next-generation sequencing?<\/a><\/h4>\n<p>A key step in the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/sequencing\/next-generation-sequencing\/ion-torrent-next-generation-sequencing-workflow.html?socid=social_btb_targetseq\" target=\"_blank\" rel=\"noopener noreferrer\">NGS workflow<\/a> is preparing the input for sequencing, known as creating a library.\u00a0 An NGS library is a collection of similarly sized DNA fragments with known adapter sequences added to the 5\u2019 and 3\u2019 ends.\u00a0 A library corresponds to a single sample and multiple libraries, each with their own unique adapter sequences, can be pooled and sequenced in the same sequencing run. Here we\u00a0focus on the four basic steps of NGS library preparation that can be broadly applied across different preparation methods.<\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/_yC0Bzw3WbQ?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<h4><a href=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/next-generation-sequencing-for-gene-expression-analysis-seq-it-out-11\/\" target=\"_blank\" rel=\"noopener noreferrer\">Can I use\u00a0Next-Generation Sequencing for\u00a0gene expression analysis?<\/a><\/h4>\n<p>Do you perform <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr.html?socid=social_btb_genequant\" target=\"_blank\" rel=\"noopener noreferrer\">qPCR<\/a> as a routine part of your research? Are you interested in <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/gene-expression-analysis-genotyping\/gene-expression-profiling.html?socid=social_btb_targetseq\" target=\"_blank\" rel=\"noopener noreferrer\">gene expression<\/a>, but haven\u2019t yet looked into how next generation sequencing or NGS can help you out?\u00a0Next generation sequencing, also known as NGS, provides opportunities to profile gene expression in a wide range of settings. Whether you are looking to discover potentially novel genes or transcripts, or study how certain genes respond to stimuli, NGS can offer a great complement to your existing methodology.<\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/gK6D-8dqw3I?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<h4><a href=\"http:\/\/admin.acceleratingscience.com\/behindthebench\/a-simple-workflow-to-confirm-your-ngs-variants-seq-it-out-17\/\" target=\"_blank\" rel=\"noopener noreferrer\">Is there a workflow to help confirm my NGS variants?<\/a><\/h4>\n<p>So you are seeing some variants in your NGS data that you are not confident about. How can you quickly re-sequence a particular locus of interest?<\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/fgy4qCY0ZKk?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p>For more <a href=\"https:\/\/www.youtube.com\/playlist?list=PLGlvFEwL2wDFVLBA1egJFwHyetpVDPExK\" target=\"_blank\" rel=\"noopener noreferrer\">SeqitOut videos<\/a> be sure to subscribe to the <a href=\"https:\/\/www.youtube.com\/channel\/UCfUs2fCDhx07fkszsJ0jOcA\">Thermo Fisher Scientific YouTube Channel<\/a> to stay up to date on the latest episodes.<\/p>\n<p>Have more questions? We&#8217;ll help you get them answered! Submit your questions at\u00a0<a href=\"http:\/\/www.thermofisher.com\/ask?socid=social_btb\" target=\"_blank\" rel=\"noopener noreferrer\">thermofisher.com\/ask<\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>With the advent of Next-Generation Sequencing (NGS), more and more researchers are turning to sequencing\u00a0to better\u00a0understand genetics and gene expression. It often seems like a daunting task to trust your precious rare samples for analysis on an instrument you\u00a0don&#8217;t have much experience using. Our SeqitOut video series tackles all things sequencing (from NGS to Sanger).\u00a0To<\/p>\n","protected":false},"author":120,"featured_media":8815,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[108],"tags":[17,11,6,43,133,176,74],"division":[],"class_list":{"0":"post-8687","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-lab-tips-and-tricks","8":"tag-applied-biosystems","9":"tag-ion-torrent","10":"tag-next-generation-sequencing","11":"tag-pcr","12":"tag-quantitative-pcr","13":"tag-seqitout","14":"tag-sequencing","15":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>5 Things to Help Start Your NGS Experiments - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/5-things-to-help-start-your-ngs-experiments\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"5 Things to Help Start Your NGS Experiments\" \/>\n<meta property=\"og:description\" content=\"With the advent of Next-Generation Sequencing (NGS), more and more researchers are turning to sequencing\u00a0to better\u00a0understand genetics and gene expression. 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