{"id":8784,"date":"2014-05-22T20:09:30","date_gmt":"2014-05-22T20:09:30","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/behindthebench\/?p=8784"},"modified":"2022-07-19T12:44:16","modified_gmt":"2022-07-19T12:44:16","slug":"chip-seq-using-the-ion-proton-system-and-the-ion-p1-chip-unveils-dendritic-cell-regulation","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/chip-seq-using-the-ion-proton-system-and-the-ion-p1-chip-unveils-dendritic-cell-regulation\/","title":{"rendered":"ChIP-Seq Using the Ion Proton System and the Ion PI Chip Unveils Dendritic Cell Regulation"},"content":{"rendered":"

\"Ion<\/a>Dendritic antigen-presenting cells help regulate immune system functions through transcription factors like Irf4, which is needed for proper CD11b+<\/sup>\u00a0dendritic cell lineage development. Malfunctions in this development have been linked to various inflammatory diseases including asthma. But the epigenetic connections between CD11b+<\/sup>\u00a0immune cell specialization and inflammatory disease are virtually unknown.<\/p>\n

Using the\u00a0Ion Proton System<\/a>\u00a0and the PI\u2122 Chip, a team from the\u00a0National Jewish Health Center for Genes, Environment and Health<\/a>\u00a0in Denver, Colorado, in collaboration with the Ion Torrent\u2122 group of Thermo Fisher Scientific developed\u00a0chromatin immunoprecipitation<\/a>\u00a0sequencing (ChIP-seq) on the Ion Proton\u2122 sequencing platform. They created an unbiased, genome-wide view of transcription factor binding sites of Irf4, using fewer than 5 million flow-sorted CD11b+<\/sup>\u00a0dendritic cells. (The poster doesn\u2019t indicate this explicitly, but the\u00a0MAGnify\u2122 ChIP Kit<\/a>\u00a0was used for the IP.) This analysis identified Irf4 gene targets that were expressed in CD11b+<\/sup>\u00a0as well as DC103+<\/sup>\u00a0dendritic cells, which may constitute a regulation program for Irf4 control of dendritic cell lineage development.<\/p>\n

The team presented their findings at the recent annual meeting of the\u00a0American Society of Biochemistry and Molecular Biology<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"

Dendritic antigen-presenting cells help regulate immune system functions through transcription factors like Irf4, which is needed for proper CD11b+\u00a0dendritic cell lineage development. Malfunctions in this development have been linked to various inflammatory diseases including asthma. But the epigenetic connections between CD11b+\u00a0immune cell specialization and inflammatory disease are virtually unknown. Using the\u00a0Ion Proton System\u00a0and the PI\u2122<\/p>\n","protected":false},"author":120,"featured_media":8790,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[135],"tags":[199,11,6],"division":[],"class_list":{"0":"post-8784","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-immunology","8":"tag-chromatin-immunoprecipitation","9":"tag-ion-torrent","10":"tag-next-generation-sequencing","11":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nChIP-Seq Using the Ion Proton System and the Ion PI Chip Unveils Dendritic Cell Regulation - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/chip-seq-using-the-ion-proton-system-and-the-ion-p1-chip-unveils-dendritic-cell-regulation\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"ChIP-Seq Using the Ion Proton System and the Ion PI Chip Unveils Dendritic Cell Regulation\" \/>\n<meta property=\"og:description\" content=\"Dendritic antigen-presenting cells help regulate immune system functions through transcription factors like Irf4, which is needed for proper CD11b+\u00a0dendritic cell lineage development. 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