{"id":9223,"date":"2015-02-24T21:10:05","date_gmt":"2015-02-24T21:10:05","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/behindthebench\/?p=9223"},"modified":"2020-05-28T19:10:35","modified_gmt":"2020-05-28T19:10:35","slug":"how-can-you-analyze-a-single-cell","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/","title":{"rendered":"How can you analyze a single cell?"},"content":{"rendered":"<div class=\"jive-rendered-content\">\n<p><strong>A novel technology to automate single-cell and cell-free DNA analysis<\/strong><\/p>\n<p>There is continued, growing interest in the <a href=\"https:\/\/ioncommunity.thermofisher.com\/servlet\/JiveServlet\/showImage\/38-1455-21177\/single+cell.PNG\"><img loading=\"lazy\" decoding=\"async\" class=\"image-0 jive-image alignright\" src=\"https:\/\/ioncommunity.thermofisher.com\/servlet\/JiveServlet\/downloadImage\/38-1455-21177\/450-189\/single+cell.PNG\" alt=\"single cell.PNG\" width=\"450\" height=\"189\" \/><\/a>analysis of single cells. For example, in 2009, Thermo Fisher Scientific\u2019s scientists collaborated with a group at the Wellcome Trust Cancer Research Institute (Cambridge, UK) for <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/sequencing\/rna-sequencing\/targeted-rna-sequencing-ion-torrent-next-generation-sequencing.html?cid=social_btb_targetseq\" rel=\"nofollow\">whole-transcriptome RNA-Seq from a single cell <\/a>(for more information about this work an <a href=\"https:\/\/tools.thermofisher.com\/content\/sfs\/brochures\/cms_065392.pdf?cid=social_btb_targetseq\" rel=\"nofollow\">Application Note is available<\/a>). Whether for cancer research (the metastatic process involved sheds cancer cells in circulation), induced Pluripotent Stem Cell (iPSC) research for potential regenerative medicine applications, or research on the frontiers of neuroscience (as discussed recently in this blog post about <a href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/brain-research-through-advancing-innovative-neurotechnologies-brain-initiative\/\">the BRAIN Initiative<\/a>), single-cell analysis will be a major area of both research focus and technology development in the future.<\/p>\n<p>Recently Thermo Fisher Scientific announced <a class=\"jive-link-external-small\" href=\"http:\/\/www.businesswire.com\/news\/home\/20150113005526\/en\/Thermo-Fisher-Scientific-Signs-Distribution-Agreement-Cynvenio\" rel=\"nofollow\">an agreement with Cynvenio Biosystems Inc.<\/a> to distribute their LiquidBiopsy<sup>TM<\/sup> Platform.\u00a0 This is an automated system of specialized collection tubes for sample collection, microfluidic chips for their processing, and magnetic separation using antibody-labeled particles. Samples are stable in the collection tubes for up to four days, and rare cells can be enumerated, visualized microscopically, and processed for downstream NGS analysis.<\/p>\n<p>Visual inspection by Microscopy is important, as recently it was reported that in a mouse model with mammary tumors, <a class=\"jive-link-external-small\" href=\"http:\/\/www.ncbi.nlm.nih.gov\/pubmed\/25171411\" rel=\"nofollow\">CTC clusters play an important role in metastases.<\/a><\/p>\n<p>The LiquidBiopsy\u2122 Platform supports circulating tumor cell (CTC) recovery rates <a class=\"jive-link-external-small\" href=\"https:\/\/www.genomeweb.com\/clinical-genomics\/cynvenio-offering-ngs-analysis-isolated-circulating-tumor-cells-using-ampliseq-p\" rel=\"nofollow\">on the order of 90% and non-specific capture (i.e. \u2018non-target\u2019 background) of less than 80 cells\/mL<\/a> of sample.\u00a0 The platform along with the strength of <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/brands\/ion-torrent.html?cid=social_btb_targetseq\" rel=\"nofollow\">Ion Torrent Sequencing System<\/a> approach offers very fast turnaround.\u00a0 The LiquidBiopsy\u2122 Platform is part of a comprehensive, cost-effective, sample to variant workflow for automated isolation, enumeration, and analysis of rare cells.\u00a0 It is unique among new technologies for isolating CTCs in two ways: this system enables analysis of CTCs, cfDNA, and germline nucleic acids on a single Ion 318\u2122 Chip, and the workflow includes sample collection through targeted sequencing data in two days.<\/p>\n<p>Additionally, the platform offers cell-free DNA analysis, an approach\u00a0 using Ion AmpliSeq\u2122 technology and QuantStudio<sup>\u00ae<\/sup> 3D Digital PCR technology.<\/p>\n<p>If you are interested in more information about the LiquidBiopsy\u2122 platform offering, please contact your local <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/technical-resources\/contact-us.html?cid=social_btb_targetseq\" rel=\"nofollow\">Thermo Fisher Scientific Genetic Analysis representative<\/a>.<\/p>\n<\/div>\n","protected":false},"excerpt":{"rendered":"<p>A novel technology to automate single-cell and cell-free DNA analysis There is continued, growing interest in the analysis of single cells. For example, in 2009, Thermo Fisher Scientific\u2019s scientists collaborated with a group at the Wellcome Trust Cancer Research Institute (Cambridge, UK) for whole-transcriptome RNA-Seq from a single cell (for more information about this work<\/p>\n","protected":false},"author":120,"featured_media":9224,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[54],"tags":[153,11,6,43,55,161,157,162],"division":[],"class_list":{"0":"post-9223","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-cancer-research","8":"tag-genomic-variants","9":"tag-ion-torrent","10":"tag-next-generation-sequencing","11":"tag-pcr","12":"tag-peripheral-monitoring","13":"tag-single-cell-analysis","14":"tag-targeted-rna-sequencing","15":"tag-whole-transcriptome","16":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>How can you analyze a single cell? - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"How can you analyze a single cell?\" \/>\n<meta property=\"og:description\" content=\"A novel technology to automate single-cell and cell-free DNA analysis There is continued, growing interest in the analysis of single cells. For example, in 2009, Thermo Fisher Scientific\u2019s scientists collaborated with a group at the Wellcome Trust Cancer Research Institute (Cambridge, UK) for whole-transcriptome RNA-Seq from a single cell (for more information about this work\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2015-02-24T21:10:05+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-05-28T19:10:35+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/single-cell-e1478208328125.png\" \/>\n\t<meta property=\"og:image:width\" content=\"353\" \/>\n\t<meta property=\"og:image:height\" content=\"294\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Behind The Bench Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Behind The Bench Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/\"},\"author\":{\"name\":\"Behind The Bench Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"headline\":\"How can you analyze a single cell?\",\"datePublished\":\"2015-02-24T21:10:05+00:00\",\"dateModified\":\"2020-05-28T19:10:35+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/\"},\"wordCount\":366,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2016\\\/11\\\/single-cell-e1478208328125.png\",\"keywords\":[\"Genomic Variants\",\"Ion Torrent\",\"Next Generation Sequencing\",\"PCR\",\"Peripheral monitoring\",\"single cell analysis\",\"Targeted RNA Sequencing\",\"Whole Transcriptome\"],\"articleSection\":[\"Cancer Research\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/\",\"name\":\"How can you analyze a single cell? - Behind the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#website\"},\"primaryImageOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/#primaryimage\"},\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2016\\\/11\\\/single-cell-e1478208328125.png\",\"datePublished\":\"2015-02-24T21:10:05+00:00\",\"dateModified\":\"2020-05-28T19:10:35+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/\"]}]},{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/#primaryimage\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2016\\\/11\\\/single-cell-e1478208328125.png\",\"contentUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2016\\\/11\\\/single-cell-e1478208328125.png\",\"width\":353,\"height\":294},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/how-can-you-analyze-a-single-cell\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"How can you analyze a single cell?\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/\",\"name\":\"Behind the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\",\"name\":\"Behind The Bench Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"caption\":\"Behind The Bench Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/behind-the-bench-staff\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"How can you analyze a single cell? - Behind the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/","og_locale":"en_US","og_type":"article","og_title":"How can you analyze a single cell?","og_description":"A novel technology to automate single-cell and cell-free DNA analysis There is continued, growing interest in the analysis of single cells. For example, in 2009, Thermo Fisher Scientific\u2019s scientists collaborated with a group at the Wellcome Trust Cancer Research Institute (Cambridge, UK) for whole-transcriptome RNA-Seq from a single cell (for more information about this work","og_url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/","og_site_name":"Behind the Bench","article_publisher":"https:\/\/www.facebook.com\/thermofisher","article_published_time":"2015-02-24T21:10:05+00:00","article_modified_time":"2020-05-28T19:10:35+00:00","og_image":[{"width":353,"height":294,"url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/single-cell-e1478208328125.png","type":"image\/png"}],"author":"Behind The Bench Staff","twitter_card":"summary_large_image","twitter_creator":"@thermofisher","twitter_site":"@thermofisher","twitter_misc":{"Written by":"Behind The Bench Staff","Est. reading time":"2 minutes"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/"},"author":{"name":"Behind The Bench Staff","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c"},"headline":"How can you analyze a single cell?","datePublished":"2015-02-24T21:10:05+00:00","dateModified":"2020-05-28T19:10:35+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/"},"wordCount":366,"commentCount":0,"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/single-cell-e1478208328125.png","keywords":["Genomic Variants","Ion Torrent","Next Generation Sequencing","PCR","Peripheral monitoring","single cell analysis","Targeted RNA Sequencing","Whole Transcriptome"],"articleSection":["Cancer Research"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/","url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/","name":"How can you analyze a single cell? - Behind the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#website"},"primaryImageOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/#primaryimage"},"image":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/#primaryimage"},"thumbnailUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/single-cell-e1478208328125.png","datePublished":"2015-02-24T21:10:05+00:00","dateModified":"2020-05-28T19:10:35+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/"]}]},{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/#primaryimage","url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/single-cell-e1478208328125.png","contentUrl":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/single-cell-e1478208328125.png","width":353,"height":294},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/how-can-you-analyze-a-single-cell\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/behindthebench\/"},{"@type":"ListItem","position":2,"name":"How can you analyze a single cell?"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#website","url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/","name":"Behind the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/behindthebench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c","name":"Behind The Bench Staff","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","caption":"Behind The Bench Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/behind-the-bench-staff\/"}]}},"taxonomy_info":{"category":[{"value":54,"label":"Cancer Research"}],"post_tag":[{"value":153,"label":"Genomic Variants"},{"value":11,"label":"Ion Torrent"},{"value":6,"label":"Next Generation Sequencing"},{"value":43,"label":"PCR"},{"value":55,"label":"Peripheral monitoring"},{"value":161,"label":"single cell analysis"},{"value":157,"label":"Targeted RNA Sequencing"},{"value":162,"label":"Whole Transcriptome"}]},"featured_image_src_large":["https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/single-cell-e1478208328125.png",353,294,false],"author_info":{"display_name":"Behind The Bench Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/behind-the-bench-staff\/"},"comment_info":0,"category_info":[{"term_id":54,"name":"Cancer Research","slug":"cancer-research","term_group":0,"term_taxonomy_id":54,"taxonomy":"category","description":"","parent":0,"count":142,"filter":"raw","meta":[],"cat_ID":54,"category_count":142,"category_description":"","cat_name":"Cancer Research","category_nicename":"cancer-research","category_parent":0}],"tag_info":[{"term_id":153,"name":"Genomic Variants","slug":"genomic-variants","term_group":0,"term_taxonomy_id":153,"taxonomy":"post_tag","description":"","parent":0,"count":68,"filter":"raw","meta":[]},{"term_id":11,"name":"Ion Torrent","slug":"ion-torrent","term_group":0,"term_taxonomy_id":11,"taxonomy":"post_tag","description":"","parent":0,"count":167,"filter":"raw","meta":[]},{"term_id":6,"name":"Next Generation Sequencing","slug":"next-generation-sequencing","term_group":0,"term_taxonomy_id":6,"taxonomy":"post_tag","description":"","parent":0,"count":170,"filter":"raw","meta":[]},{"term_id":43,"name":"PCR","slug":"pcr","term_group":0,"term_taxonomy_id":43,"taxonomy":"post_tag","description":"","parent":0,"count":142,"filter":"raw","meta":[]},{"term_id":55,"name":"Peripheral monitoring","slug":"peripheral-monitoring","term_group":0,"term_taxonomy_id":55,"taxonomy":"post_tag","description":"","parent":0,"count":24,"filter":"raw","meta":[]},{"term_id":161,"name":"single cell analysis","slug":"single-cell-analysis","term_group":0,"term_taxonomy_id":161,"taxonomy":"post_tag","description":"","parent":0,"count":8,"filter":"raw","meta":[]},{"term_id":157,"name":"Targeted RNA Sequencing","slug":"targeted-rna-sequencing","term_group":0,"term_taxonomy_id":157,"taxonomy":"post_tag","description":"","parent":0,"count":25,"filter":"raw","meta":[]},{"term_id":162,"name":"Whole Transcriptome","slug":"whole-transcriptome","term_group":0,"term_taxonomy_id":162,"taxonomy":"post_tag","description":"","parent":0,"count":8,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":19509,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/cfdna-fragmentomics-analysis-qpcr-cancer-monitoring\/","url_meta":{"origin":9223,"position":0},"title":"qPCR-based fragmentomics for monitoring cancer disease progression","author":"Behind The Bench Staff","date":"March 5, 2026","format":false,"excerpt":"As fragmentomics gains momentum in cancer liquid biopsy research, attention is increasingly turning to how these structural cfDNA signals can be measured in a way that is both sensitive and practical. Established qPCR platforms offer an alternative to sequencing-based approaches for capturing fragment size information with high precision. Using a\u2026","rel":"","context":"In &quot;Bioinformatics&quot;","block_context":{"text":"Bioinformatics","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/bioinformatics\/"},"img":{"alt_text":"Scientist pipetting into a 96 well plate during qPCR workflow for cell free DNA fragmentomics analysis","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Picture1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Picture1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Picture1.png?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":19911,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/multiplex-mirna-profiling-precision-oncology\/","url_meta":{"origin":9223,"position":1},"title":"A scalable multiplex miRNA profiling workflow for precision oncology research","author":"Ashleigh Barlow","date":"August 18, 2026","format":false,"excerpt":"The promise of precision oncology rests on our ability to reliably monitor tumor biology in real time. Circulating microRNAs (miRNAs) offer that opportunity, serving as potentially stable, non-invasive biomarkers linked to disease progression and therapeutic response; however, their adoption in routine practice remains constrained by technical hurdles. (1,2) Because cancer\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"Scientist pipetting a laboratory sample during molecular biomarker research.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/lisa-blog-picture.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/lisa-blog-picture.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/lisa-blog-picture.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/08\/lisa-blog-picture.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":19810,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/integration-site-analysis-gene-therapy-safety\/","url_meta":{"origin":9223,"position":2},"title":"Genotoxicity, vectors, and the power of integration site analysis","author":"Ashleigh Barlow","date":"June 23, 2026","format":false,"excerpt":"Article Summary In this episode of Absolute Gene-ius, Michael Stump, PhD, President of ProtaGene, and Matteo Franco, Lead Scientist at ProtaGene, discuss how integration site analysis is helping researchers evaluate genotoxicity risks in cell and gene therapy workflows. Their conversation explores how viral vectors integrate into the genome, why integration\u2026","rel":"","context":"In &quot;Gene Editing&quot;","block_context":{"text":"Gene Editing","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/gene-editing\/"},"img":{"alt_text":"Matteo Franco, Lead Scientist at ProtaGene, discussing integration site analysis and genotoxicity assessment","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/FRANCO-headshot_Jordan-Ruggieri.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19447,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/philadelphia-chromosome-like-all-digital-pcr\/","url_meta":{"origin":9223,"position":3},"title":"Unlocking Cancer\u2019s Genetic Secrets with Digital PCR","author":"Behind The Bench Staff","date":"February 27, 2026","format":false,"excerpt":"In this episode of Absolute Gene-ius, we explore how digital PCR (dPCR) is transforming leukemia research with Valeria Rangel, a PhD candidate at the University of California, Irvine. Her work focuses on Philadelphia chromosome-like (Ph-like) B-cell acute lymphoblastic leukemia (ALL), a rare, aggressive subtype that disproportionately affects Hispanic populations. By\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/RANGEL-headshot-1.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/RANGEL-headshot-1.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/RANGEL-headshot-1.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/11\/RANGEL-headshot-1.jpg?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":19579,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/liquid-biopsy-cancer-research-ngs-pcr\/","url_meta":{"origin":9223,"position":4},"title":"From Discovery to Monitoring: How Sequencing and PCR Work Together in Oncology Research","author":"Behind The Bench Staff","date":"March 24, 2026","format":false,"excerpt":"Scientific, joins the Absolute Gene-ius podcast to discuss how molecular technologies are advancing precision oncology research. With experience spanning clinical laboratory research, digital PCR research applications, and next-generation sequencing (NGS), Dr. Sollweck shares how scientists are addressing critical unmet needs in cancer research. From liquid biopsy research strategies to minimal\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/03\/Sollweck-headshot-200x250_Jordan-Ruggieri.jpeg?resize=350%2C200&ssl=1","width":350,"height":200},"classes":[]},{"id":19193,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/advancing-cancer-cell-based-assays-with-assay-ready-tumoroids\/","url_meta":{"origin":9223,"position":5},"title":"Advancing Cancer Cell-Based Assays with Assay-Ready Tumoroids","author":"Behind The Bench Staff","date":"October 28, 2025","format":false,"excerpt":"Cancer research is continually evolving, and patient-derived tumoroids are playing a crucial role in this progress. These three-dimensional (3D) cell culture models, also known as cancer organoids, closely mimic the genetic profile, gene expression patterns, and architecture of actual tumors. Therefore, tumoroids are increasingly being used for basic research, drug\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"Microscopic image of HuLu051921 tumoroids stained with Hematoxylin and Eosin at 20x magnification, field 1, bottom section of the slide","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/10\/microscopic-tumoroid-cells.png?resize=1050%2C600&ssl=1 3x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/single-cell-e1478208328125.png","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/9223","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/users\/120"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/comments?post=9223"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/9223\/revisions"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media\/9224"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media?parent=9223"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/categories?post=9223"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/tags?post=9223"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/division?post=9223"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}