{"id":9247,"date":"2015-07-22T18:17:27","date_gmt":"2015-07-22T18:17:27","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/behindthebench\/?p=9247"},"modified":"2020-05-28T19:20:36","modified_gmt":"2020-05-28T19:20:36","slug":"whats-the-right-master-mix-for-your-qpcr-experiment-lets-ask-taqman","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/whats-the-right-master-mix-for-your-qpcr-experiment-lets-ask-taqman\/","title":{"rendered":"What&#8217;s the Right Master Mix for Your qPCR Experiment? Let&#8217;s Ask TaqMan"},"content":{"rendered":"<p>Which master mix do you choose? How do you know it\u2019s the right one? Choosing the right master mix is an important step when performing your experiment because you want to make sure it is completely optimized for success.<\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/ei-eCjwpVVk?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p class=\"TableParagraph\">It\u2019s a constant argument. There have been rap battles that tear labs apart\u2026 Which do you use, <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-reagents\/sybr-green-real-time-master-mixes.html?icid=fr-sybrmix-main?icid=lsg_gsd_CP1188_blog_asktaqman\" target=\"_blank\" rel=\"noopener noreferrer\">SYBR\u2122<\/a> or <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-reagents\/taqman-real-time-master-mixes.html?icid=fr-taqmanmix-main?icid=lsg_gsd_CP1188_blog_asktaqman\" target=\"_blank\" rel=\"noopener noreferrer\">TaqMan\u2122<\/a> master mix?<\/p>\n<p class=\"TableParagraph\">So what is a master mix anyway? It\u2019s a mixture of polymerase, salts, magnesium, dNTPs, and optimized reaction buffer. Your template and primers are the only additions necessary to perform qPCR. Different formulations are good for different situations or applications such as Genotyping,\u00a0gene expression, MicroRNA quantification, and virus detection<\/p>\n<p>We offer two types of detection technologies when performing <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/qpcr-education.html?icid=lsg_gsd_CP1188_blog_asktaqman\" target=\"_blank\" rel=\"noopener noreferrer\">Real-time PCR (or qPCR)<\/a>. The first is <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-reagents\/sybr-green-real-time-master-mixes.html?icid=lsg_gsd_CP1188_blog_asktaqman\" target=\"_blank\" rel=\"noopener noreferrer\">Applied Biosystems SYBR\u2122 Green<\/a>, a non-specific master mix DNA binding dye. Specifically, it is based on two hybridization events, the forward and reverse primer\u00a0 The second\u00a0 technology is TaqMan master mix which bases its\u00a0 detection on 5\u2019 nuclease activity.\u00a0 Specifically, it requires three hybridization events:\u00a0 two PCR primers and one probe, with\u00a0 hydrolysis of the probe during extension of the primers.<\/p>\n<p class=\"TableParagraph\">The first thing to consider when choosing a master mix is what type of application you are working with.\u00a0 For example, let\u2019s focus on <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/taqman-gene-expression.html?icid=lsg_gsd_CP1188_blog_asktaqman\" target=\"_blank\" rel=\"noopener noreferrer\">Gene Expression<\/a>. While both technologies can be used, sometimes the experiment is better suited for SYBR\u00ae chemistry and sometimes it\u2019s better suited to <a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-reagents\/taqman-real-time-master-mixes.html?icid=lsg_gsd_CP1188_blog_asktaqman\" target=\"_blank\" rel=\"noopener noreferrer\">TaqMan\u2122 chemistry<\/a>. TaqMan\u2122 chemistry offers the convenience of millions of pre-designed assays and greater levels of specificity but SYBR\u00ae offers flexibility for species that aren\u2019t pre-defined which is a value option. SYBR\u00ae Green Master Mix includes SYBR\u00ae green assays in them, whereas TaqMan require the addition of a label probe. Let\u2019s take a look at what else you have to consider when choosing your master mix. For your gene expression experiment you decide if you need high specificity and a predesigned assay, if so, it\u2019s time for Taqman chemistry, but which one? We can help, but we have a few questions for you: What format are you going to use? 1 step or 2 step ? What mode are you going to run? Fast or standard? Once you select the options that make up your particular experiment, the recommended Master Mix will appear in the online selection tool.<\/p>\n<p>Have more than one experiment? This same selection tool can help you determine which Master mix is recommended for each situation, Whether you have different types of analysis or just different samples types and sizes, we\u2019ll help find the right master mix for you.<\/p>\n<p class=\"TableParagraph\">If you have more questions about master mix or other qPCR questions, remember, just ask Taqman. Submit your question at <a href=\"http:\/\/www.thermofisher.com\/ask?cid=social_btb_genequant\" target=\"_blank\" rel=\"nofollow noopener noreferrer\">https:\/\/www.thermofisher.com\/ask<\/a>\u00a0and subscribe to our channel to see more videos like this.<\/p>\n<p>I\u2019ll see you next time.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Which master mix do you choose? How do you know it\u2019s the right one? Choosing the right master mix is an important step when performing your experiment because you want to make sure it is completely optimized for success. It\u2019s a constant argument. There have been rap battles that tear labs apart\u2026 Which do you<\/p>\n","protected":false},"author":112,"featured_media":9248,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[108],"tags":[17,18,104,43,46],"division":[],"class_list":{"0":"post-9247","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-lab-tips-and-tricks","8":"tag-applied-biosystems","9":"tag-ask-taqman","10":"tag-gene-expression","11":"tag-pcr","12":"tag-real-time-pcr","13":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>What&#039;s the Right Master Mix for Your qPCR Experiment? Let&#039;s Ask TaqMan - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/whats-the-right-master-mix-for-your-qpcr-experiment-lets-ask-taqman\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"What&#039;s the Right Master Mix for Your qPCR Experiment? Let&#039;s Ask TaqMan\" \/>\n<meta property=\"og:description\" content=\"Which master mix do you choose? How do you know it\u2019s the right one? Choosing the right master mix is an important step when performing your experiment because you want to make sure it is completely optimized for success. It\u2019s a constant argument. There have been rap battles that tear labs apart\u2026 Which do you\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/whats-the-right-master-mix-for-your-qpcr-experiment-lets-ask-taqman\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2015-07-22T18:17:27+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-05-28T19:20:36+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/at.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"1578\" \/>\n\t<meta property=\"og:image:height\" content=\"778\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Mike Troutman\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Mike Troutman\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"2 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/whats-the-right-master-mix-for-your-qpcr-experiment-lets-ask-taqman\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/whats-the-right-master-mix-for-your-qpcr-experiment-lets-ask-taqman\\\/\"},\"author\":{\"name\":\"Mike Troutman\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/4d15e6a9e79225dd37a71a333d816e64\"},\"headline\":\"What&#8217;s the Right Master Mix for Your qPCR Experiment? 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