{"id":9372,"date":"2014-12-17T19:10:17","date_gmt":"2014-12-17T19:10:17","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/behindthebench\/?p=9372"},"modified":"2016-12-06T20:58:57","modified_gmt":"2016-12-06T20:58:57","slug":"analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\/","title":{"rendered":"Analyzing the microbiome in HIV-infected samples using 16S metagnomics: Sandra Pinto Cardoso Genetic Solutions Tour San Francisco"},"content":{"rendered":"<p>Dr. Cardoso of the National Institute of Respiratory Diseases (Mexico City, Mexico) uses 16S rRNA sequencing to understand how the gut microbiome composition and its potential impact on HIV. She also discusses her use of the new <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/sequencing\/dna-sequencing\/microbial-sequencing\/microbial-identification-ion-torrent-next-generation-sequencing\/ion-16s-metagenomics-solution.html?cid=social_btb_targetseq\" rel=\"nofollow\">Ion 16S\u2122 Metagenomics Kit<\/a> to classify the gut microbime composition and how the use of 7 variable regions compares to the single variable region she was using previously.<\/p>\n<p>&nbsp;<\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/hiR53pgh93M?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p>We\u2019ve written before about <a href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/16s-rrna-analysis-finally-arrives\/\">16S metagenomics here<\/a> as well as this post regarding <a href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/16s-rrna-bacterial-community-profiling-platform-comparison-published-in-applied-environmental-microbiology\/\">a recent platform evaluation<\/a>.\u00a0 Get more information about the <a href=\"https:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/sequencing\/dna-sequencing\/microbial-sequencing\/microbial-identification-ion-torrent-next-generation-sequencing\/ion-16s-metagenomics-solution.html?cid=social_btb_targetseq\" rel=\"nofollow\">Ion 16S Metagenomics Solution here<\/a>.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Dr. Cardoso of the National Institute of Respiratory Diseases (Mexico City, Mexico) uses 16S rRNA sequencing to understand how the gut microbiome composition and its potential impact on HIV. She also discusses her use of the new Ion 16S\u2122 Metagenomics Kit to classify the gut microbime composition and how the use of 7 variable regions<\/p>\n","protected":false},"author":120,"featured_media":9202,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[113,135,67,84],"tags":[103,11,6],"division":[],"class_list":{"0":"post-9372","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-events-and-tradeshows","8":"category-immunology","9":"category-infectious-diseases","10":"category-metagenomics","11":"tag-16s-rrna-microbial","12":"tag-ion-torrent","13":"tag-next-generation-sequencing","14":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Analyzing the microbiome in HIV-infected samples using 16S metagnomics: Sandra Pinto Cardoso Genetic Solutions Tour San Francisco - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Analyzing the microbiome in HIV-infected samples using 16S metagnomics: Sandra Pinto Cardoso Genetic Solutions Tour San Francisco\" \/>\n<meta property=\"og:description\" content=\"Dr. Cardoso of the National Institute of Respiratory Diseases (Mexico City, Mexico) uses 16S rRNA sequencing to understand how the gut microbiome composition and its potential impact on HIV. She also discusses her use of the new Ion 16S\u2122 Metagenomics Kit to classify the gut microbime composition and how the use of 7 variable regions\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2014-12-17T19:10:17+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2016-12-06T20:58:57+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/Ion_World_Tour-e1478116420288.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"226\" \/>\n\t<meta property=\"og:image:height\" content=\"199\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Behind The Bench Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Behind The Bench Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\\\/\"},\"author\":{\"name\":\"Behind The Bench Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"headline\":\"Analyzing the microbiome in HIV-infected samples using 16S metagnomics: Sandra Pinto Cardoso Genetic Solutions Tour San Francisco\",\"datePublished\":\"2014-12-17T19:10:17+00:00\",\"dateModified\":\"2016-12-06T20:58:57+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\\\/\"},\"wordCount\":118,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2016\\\/11\\\/Ion_World_Tour-e1478116420288.jpg\",\"keywords\":[\"16S rRNA Microbial\",\"Ion Torrent\",\"Next Generation Sequencing\"],\"articleSection\":[\"Events and Tradeshows\",\"Immunology\",\"Infectious Diseases\",\"Metagenomics\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/analyzing-the-microbiome-in-hiv-infected-samples-using-16s-metagnomics-sandra-pinto-cardoso-genetic-solutions-tour-san-francisco\\\/\",\"name\":\"Analyzing the microbiome in HIV-infected samples using 16S metagnomics: Sandra Pinto Cardoso Genetic Solutions Tour San Francisco - 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Every downstream application, from PCR to next-generation sequencing (NGS), depends on starting with nucleic acids that are accurately quantified and free from contaminants that could compromise results. Establishing a consistent quality control workflow helps laboratories improve reproducibility\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"A pipette drops a liquid into a petri dish with a dna sequence background, science concept. 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