{"id":9413,"date":"2014-12-23T00:53:32","date_gmt":"2014-12-23T00:53:32","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/behindthebench\/?p=9413"},"modified":"2020-05-28T19:08:29","modified_gmt":"2020-05-28T19:08:29","slug":"rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\/","title":{"rendered":"Rare mutation detection using the QuantStudio\u00ae 3D Digital PCR System"},"content":{"rendered":"

Searching for rare mutations in a majority wild-type background is a challenge when studying tumorigenesis. Earlier this year at the European Society for Human Genetics we interviewed a researcher using the QuantStudio\u00ae 3D Digital PCR System for rare KRAS mutations in cell-free DNA samples, and at that same meeting we also interviewed one of our internal application scientists who was looking at leukemia chimerism in a poster she presented.\"rma\"<\/p>\n

We now have the QuantStudio\u00ae<\/sup> 3D Rare Mutation Analysis Solution<\/a> which includes 38 QuantStudio 3D wet-lab-validated TaqMan\u00ae<\/sup> SNP Genotyping Assays assays along with streamlined analysis for detection of somatic mutations as low as 0.1% in prevalence.<\/p>\n

These custom TaqMan\u00ae<\/sup> SNP Genotyping Assays cover key somatic mutations in the KRAS, BRAF, EGFR, PIK3CA, and JAK2 genes, based on both frequency of occurrence in tumorigenesis as well as overlap with the Ion AmpliSeq\u2122 Cancer Hotspot Panel v2. The list of verified assays are available<\/a> for download here, and we plan to routinely expand this list.<\/p>\n

If you\u2019d like to hear Dr. Iain Russell at the recent American Society for Human Genetics meeting discussing this product, you can find the interview here<\/a>, and for more information you can access the product pages here<\/a> (including a product overview bulletin PDF<\/a>, a user guide and ordering info<\/p>\n","protected":false},"excerpt":{"rendered":"

Searching for rare mutations in a majority wild-type background is a challenge when studying tumorigenesis. Earlier this year at the European Society for Human Genetics we interviewed a researcher using the QuantStudio\u00ae 3D Digital PCR System for rare KRAS mutations in cell-free DNA samples, and at that same meeting we also interviewed one of our<\/p>\n","protected":false},"author":120,"featured_media":9414,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[54,101],"tags":[17,167,57,153,43],"division":[],"class_list":{"0":"post-9413","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-cancer-research","8":"category-product-news","9":"tag-applied-biosystems","10":"tag-array-plates-assays","11":"tag-digital-pcr","12":"tag-genomic-variants","13":"tag-pcr","14":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nRare mutation detection using the QuantStudio\u00ae 3D Digital PCR System - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Rare mutation detection using the QuantStudio\u00ae 3D Digital PCR System\" \/>\n<meta property=\"og:description\" content=\"Searching for rare mutations in a majority wild-type background is a challenge when studying tumorigenesis. Earlier this year at the European Society for Human Genetics we interviewed a researcher using the QuantStudio\u00ae 3D Digital PCR System for rare KRAS mutations in cell-free DNA samples, and at that same meeting we also interviewed one of our\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2014-12-23T00:53:32+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2020-05-28T19:08:29+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2016\/11\/rma.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1178\" \/>\n\t<meta property=\"og:image:height\" content=\"602\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"Behind The Bench Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Behind The Bench Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\\\/\"},\"author\":{\"name\":\"Behind The Bench Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"headline\":\"Rare mutation detection using the QuantStudio\u00ae 3D Digital PCR System\",\"datePublished\":\"2014-12-23T00:53:32+00:00\",\"dateModified\":\"2020-05-28T19:08:29+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\\\/\"},\"wordCount\":221,\"commentCount\":0,\"image\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\\\/#primaryimage\"},\"thumbnailUrl\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/wp-content\\\/uploads\\\/sites\\\/9\\\/2016\\\/11\\\/rma.png\",\"keywords\":[\"Applied Biosystems\",\"Array Plates & Assays\",\"digital PCR\",\"Genomic Variants\",\"PCR\"],\"articleSection\":[\"Cancer Research\",\"Product News\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/rare-mutation-detection-using-the-quantstudio-3d-digital-pcr-system\\\/\",\"name\":\"Rare mutation detection using the QuantStudio\u00ae 3D Digital PCR System - 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Variability in workflows and the need for robust verification steps limit widespread adoption. Dr. Matteo Allegretti, Senior Researcher at the Translational Oncology Research Unit at the IRCCS Regina\u2026","rel":"","context":"In "Cancer Research"","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"Research scientist operating laboratory equipment during liquid biopsy and biomarker research.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":19345,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/precision-oncology-diagnostics\/","url_meta":{"origin":9413,"position":3},"title":"The Expanding Role of Molecular Diagnostics in Precision Oncology","author":"Behind The Bench Staff","date":"January 13, 2026","format":false,"excerpt":"https:\/\/www.youtube.com\/watch?v=F6FfHIizJBY For Dr. Harald Bartsch, a clinical pathologist in southern Bavaria, molecular diagnostic tools have become an indispensable part of his arsenal. \u201cI believe molecular diagnostics play a crucial role in modern oncology, and I see this as the direction everything is headed,\u201d says Dr. Bartsch. 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The benefits are clear: reduced sample consumption, lower costs, faster workflows, and more data from every experiment. However, designing successful multiplex assays isn't always straightforward. Traditionally, identifying problematic assay combinations required extensive wet-lab\u2026","rel":"","context":"In "Lab Tips and Tricks"","block_context":{"text":"Lab Tips and Tricks","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/lab-tips-and-tricks\/"},"img":{"alt_text":"For Research Use Only. Not for use in diagnostic procedures. \u00a9 2026 Thermo Fisher Scientific Inc. All rights reserved. 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