{"id":9850,"date":"2014-10-21T00:24:17","date_gmt":"2014-10-21T00:24:17","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/behindthebench\/?p=9850"},"modified":"2016-12-06T00:28:42","modified_gmt":"2016-12-06T00:28:42","slug":"approach-for-limited-cell-chip-seq-on-ion-proton","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/","title":{"rendered":"Approach for limited cell ChIP-Seq on Ion Proton"},"content":{"rendered":"<p>Working with collaborators at National Jewish Health (Denver CO), Dr. Srinka Ghosh and other Applications Scientists at Thermo Fisher Scientific helped improve an existing ChIP-Seq protocol for the\u00a0<a href=\"http:\/\/www.thermofisher.com\/us\/en\/home\/life-science\/sequencing\/next-generation-sequencing\/ion-torrent-next-generation-sequencing-workflow\/ion-torrent-next-generation-sequencing-run-sequence\/ion-proton-system-for-next-generation-sequencing.html?cid=social_btb_clinonc\" rel=\"nofollow\">Ion Proton\u2122 System<\/a>, and presented their results in a poster titled \u201cApproach for limited cell ChIP-Seq on a semiconductor-based sequencing system\u201d. In addition, Dr. Brian O\u2019Connor of National Jewish Health presented data from this collaboration at a Thermo Fisher Scientific ASHG workshop. Here is an interview with Dr. Ghosh at ASHG.<\/p>\n<p><iframe loading=\"lazy\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/tBlRAfOJNVs?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; encrypted-media; gyroscope; picture-in-picture\" allowfullscreen><\/iframe><\/p>\n<p>For additional details you can access and download this ASHG poster here.\u00a0<strong><a class=\"jive-link-external-small\" href=\"http:\/\/www.slideshare.net\/ThermoFisher\/ashg-2014-chipseqsgfinal\" rel=\"nofollow\">Approach for limited cell ChIP-Seq on a semiconductor-based sequencing platform<\/a><\/strong>from\u00a0<strong><a class=\"jive-link-external-small\" href=\"http:\/\/www.slideshare.net\/ThermoFisher\" rel=\"nofollow\">Thermo Fisher Scientific<\/a><\/strong><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Working with collaborators at National Jewish Health (Denver CO), Dr. Srinka Ghosh and other Applications Scientists at Thermo Fisher Scientific helped improve an existing ChIP-Seq protocol for the\u00a0Ion Proton\u2122 System, and presented their results in a poster titled \u201cApproach for limited cell ChIP-Seq on a semiconductor-based sequencing system\u201d. In addition, Dr. Brian O\u2019Connor of National<\/p>\n","protected":false},"author":120,"featured_media":0,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[113],"tags":[150],"division":[],"class_list":{"0":"post-9850","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-events-and-tradeshows","7":"tag-ashg-2014","8":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Approach for limited cell ChIP-Seq on Ion Proton - Behind the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Approach for limited cell ChIP-Seq on Ion Proton\" \/>\n<meta property=\"og:description\" content=\"Working with collaborators at National Jewish Health (Denver CO), Dr. Srinka Ghosh and other Applications Scientists at Thermo Fisher Scientific helped improve an existing ChIP-Seq protocol for the\u00a0Ion Proton\u2122 System, and presented their results in a poster titled \u201cApproach for limited cell ChIP-Seq on a semiconductor-based sequencing system\u201d. In addition, Dr. Brian O\u2019Connor of National\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/\" \/>\n<meta property=\"og:site_name\" content=\"Behind the Bench\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2014-10-21T00:24:17+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2016-12-06T00:28:42+00:00\" \/>\n<meta name=\"author\" content=\"Behind The Bench Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:site\" content=\"@thermofisher\" \/>\n<meta name=\"twitter:label1\" content=\"Written by\" \/>\n\t<meta name=\"twitter:data1\" content=\"Behind The Bench Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data2\" content=\"1 minute\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"Article\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/#article\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/\"},\"author\":{\"name\":\"Behind The Bench Staff\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"headline\":\"Approach for limited cell ChIP-Seq on Ion Proton\",\"datePublished\":\"2014-10-21T00:24:17+00:00\",\"dateModified\":\"2016-12-06T00:28:42+00:00\",\"mainEntityOfPage\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/\"},\"wordCount\":121,\"commentCount\":0,\"keywords\":[\"ASHG 2014\"],\"articleSection\":[\"Events and Tradeshows\"],\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"CommentAction\",\"name\":\"Comment\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/#respond\"]}]},{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/\",\"url\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/\",\"name\":\"Approach for limited cell ChIP-Seq on Ion Proton - Behind the Bench\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#website\"},\"datePublished\":\"2014-10-21T00:24:17+00:00\",\"dateModified\":\"2016-12-06T00:28:42+00:00\",\"author\":{\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\"},\"breadcrumb\":{\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/www.thermofisher.com\\\/blog\\\/behindthebench\\\/approach-for-limited-cell-chip-seq-on-ion-proton\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Home\",\"item\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Approach for limited cell ChIP-Seq on Ion Proton\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#website\",\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/\",\"name\":\"Behind the Bench\",\"description\":\"\",\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Person\",\"@id\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/behindthebench\\\/#\\\/schema\\\/person\\\/9bbb2d67616274d3d4a21a584f7f4a0c\",\"name\":\"Behind The Bench Staff\",\"image\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"url\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"contentUrl\":\"https:\\\/\\\/secure.gravatar.com\\\/avatar\\\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g\",\"caption\":\"Behind The Bench Staff\"},\"url\":\"https:\\\/\\\/admin.acceleratingscience.com\\\/author\\\/behind-the-bench-staff\\\/\"}]}<\/script>\n<!-- \/ Yoast SEO Premium plugin. -->","yoast_head_json":{"title":"Approach for limited cell ChIP-Seq on Ion Proton - Behind the Bench","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/","og_locale":"en_US","og_type":"article","og_title":"Approach for limited cell ChIP-Seq on Ion Proton","og_description":"Working with collaborators at National Jewish Health (Denver CO), Dr. Srinka Ghosh and other Applications Scientists at Thermo Fisher Scientific helped improve an existing ChIP-Seq protocol for the\u00a0Ion Proton\u2122 System, and presented their results in a poster titled \u201cApproach for limited cell ChIP-Seq on a semiconductor-based sequencing system\u201d. In addition, Dr. Brian O\u2019Connor of National","og_url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/","og_site_name":"Behind the Bench","article_publisher":"https:\/\/www.facebook.com\/thermofisher","article_published_time":"2014-10-21T00:24:17+00:00","article_modified_time":"2016-12-06T00:28:42+00:00","author":"Behind The Bench Staff","twitter_card":"summary_large_image","twitter_creator":"@thermofisher","twitter_site":"@thermofisher","twitter_misc":{"Written by":"Behind The Bench Staff","Est. reading time":"1 minute"},"schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"Article","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/#article","isPartOf":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/"},"author":{"name":"Behind The Bench Staff","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c"},"headline":"Approach for limited cell ChIP-Seq on Ion Proton","datePublished":"2014-10-21T00:24:17+00:00","dateModified":"2016-12-06T00:28:42+00:00","mainEntityOfPage":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/"},"wordCount":121,"commentCount":0,"keywords":["ASHG 2014"],"articleSection":["Events and Tradeshows"],"inLanguage":"en-US","potentialAction":[{"@type":"CommentAction","name":"Comment","target":["https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/#respond"]}]},{"@type":"WebPage","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/","url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/","name":"Approach for limited cell ChIP-Seq on Ion Proton - Behind the Bench","isPartOf":{"@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#website"},"datePublished":"2014-10-21T00:24:17+00:00","dateModified":"2016-12-06T00:28:42+00:00","author":{"@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c"},"breadcrumb":{"@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/approach-for-limited-cell-chip-seq-on-ion-proton\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Home","item":"https:\/\/admin.acceleratingscience.com\/behindthebench\/"},{"@type":"ListItem","position":2,"name":"Approach for limited cell ChIP-Seq on Ion Proton"}]},{"@type":"WebSite","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#website","url":"https:\/\/admin.acceleratingscience.com\/behindthebench\/","name":"Behind the Bench","description":"","potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/admin.acceleratingscience.com\/behindthebench\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Person","@id":"https:\/\/admin.acceleratingscience.com\/behindthebench\/#\/schema\/person\/9bbb2d67616274d3d4a21a584f7f4a0c","name":"Behind The Bench Staff","image":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","url":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","contentUrl":"https:\/\/secure.gravatar.com\/avatar\/52d5c16ece4bd1c0a49a63521c2b6a25c54a3f0c33f7afed215ba7e0cdff69e3?s=96&d=mm&r=g","caption":"Behind The Bench Staff"},"url":"https:\/\/admin.acceleratingscience.com\/author\/behind-the-bench-staff\/"}]}},"taxonomy_info":{"category":[{"value":113,"label":"Events and Tradeshows"}],"post_tag":[{"value":150,"label":"ASHG 2014"}]},"featured_image_src_large":false,"author_info":{"display_name":"Behind The Bench Staff","author_link":"https:\/\/admin.acceleratingscience.com\/author\/behind-the-bench-staff\/"},"comment_info":0,"category_info":[{"term_id":113,"name":"Events and Tradeshows","slug":"events-and-tradeshows","term_group":0,"term_taxonomy_id":113,"taxonomy":"category","description":"","parent":0,"count":89,"filter":"raw","meta":[],"cat_ID":113,"category_count":89,"category_description":"","cat_name":"Events and Tradeshows","category_nicename":"events-and-tradeshows","category_parent":0}],"tag_info":[{"term_id":150,"name":"ASHG 2014","slug":"ashg-2014","term_group":0,"term_taxonomy_id":150,"taxonomy":"post_tag","description":"","parent":0,"count":10,"filter":"raw","meta":[]}],"jetpack-related-posts":[{"id":19435,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/scaling-human-relevant-oncology-testing-oncopro-tumoroids-on-screenin3ds-upscale-platform\/","url_meta":{"origin":9850,"position":0},"title":"Scaling Human-Relevant Oncology Testing: OncoPro Tumoroids on ScreenIn3D\u2019s UpScale Platform","author":"Behind The Bench Staff","date":"February 25, 2026","format":false,"excerpt":"The United States National Institutes of Health has prioritized human cell-based in vitro approaches to \u201cmodel human disease and capture human variability and patient-specific characteristics,\u201d complementing the U.S. Food and Drug Administration\u2019s goals to use advanced in vitro assays as a New Approach Methodology (NAM) that could reduce animal testing.\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/02\/HuCo021320-screen-v2-1.png?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19837,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/managing-formulation-fill-finish-in-the-car-t-cell-manufacturing-process\/","url_meta":{"origin":9850,"position":1},"title":"Managing Formulation, Fill &amp; Finish in the CAR T-Cell Manufacturing Process","author":"Behind The Bench Staff","date":"July 28, 2026","format":false,"excerpt":"Chimeric antigen receptor (CAR)-T cell therapies represent a paradigm shift in medicine, offering personalized, potentially curative treatments for cancer and other serious diseases. Unlike traditional biologics, CAR T-cell products are living therapeutics. Highly sensitive cells whose quality, safety, and efficacy depend on careful handling at every step of the manufacturing\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"Scientist working in a lab","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Hero_image_1920x1080.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Hero_image_1920x1080.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Hero_image_1920x1080.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Hero_image_1920x1080.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Hero_image_1920x1080.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Hero_image_1920x1080.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19966,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/multiplex-assay-optimization-qpcr\/","url_meta":{"origin":9850,"position":2},"title":"Building Better Multiplex Panels: Identifying Assay Interactions Early","author":"Ashleigh Barlow","date":"September 8, 2026","format":false,"excerpt":"Multiplex qPCR has transformed molecular research by enabling scientists to detect multiple targets in a single reaction. The benefits are clear: reduced sample consumption, lower costs, faster workflows, and more data from every experiment. However, designing successful multiplex assays isn't always straightforward. Traditionally, identifying problematic assay combinations required extensive wet-lab\u2026","rel":"","context":"In &quot;Lab Tips and Tricks&quot;","block_context":{"text":"Lab Tips and Tricks","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/lab-tips-and-tricks\/"},"img":{"alt_text":"For Research Use Only. Not for use in diagnostic procedures. \u00a9 2026 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/09\/Header-banner-scaled.jpg?resize=1400%2C800&ssl=1 4x"},"classes":[]},{"id":19271,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/multiplex-qpcr-basics-guide\/","url_meta":{"origin":9850,"position":3},"title":"Multiplex vs. Singleplex qPCR: What do I need to know?","author":"Ashleigh Barlow","date":"December 3, 2025","format":false,"excerpt":"Real-time PCR (qPCR) has transformed how scientists detect and quantify nucleic acid targets, offering analytical sensitivity, speed, and scalability. Many real-time PCR reactions measure a single target per well \u2014 a setup called singleplex qPCR. However, the effectiveness of real-time PCR can be increased by an approach called multiplex qPCR.\u2026","rel":"","context":"In &quot;General&quot;","block_context":{"text":"General","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/general\/"},"img":{"alt_text":"","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2025\/12\/Web-header-900-x-600-custom-web-page-size-feature-pods.png?resize=700%2C400&ssl=1 2x"},"classes":[]},{"id":19805,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/liquid-biopsy-workflow-standardization\/","url_meta":{"origin":9850,"position":4},"title":"Improving the Reliability of Liquid Biopsy in Real-World Settings Through Workflow Standardization: A Discussion with Dr. Matteo Allegretti","author":"Ashleigh Barlow","date":"June 18, 2026","format":false,"excerpt":"Liquid biopsy offers a powerful, noninvasive way to detect and track cancer, but ensuring reliability in real-world settings remains a key hurdle. Variability in workflows and the need for robust verification steps limit widespread adoption. Dr. Matteo Allegretti, Senior Researcher at the Translational Oncology Research Unit at the IRCCS Regina\u2026","rel":"","context":"In &quot;Cancer Research&quot;","block_context":{"text":"Cancer Research","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/cancer-research\/"},"img":{"alt_text":"Research scientist operating laboratory equipment during liquid biopsy and biomarker research.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/06\/Allegretti-Header.png?resize=525%2C300&ssl=1 1.5x"},"classes":[]},{"id":19828,"url":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/cyclospora-molecular-testing-gi-pathogens\/","url_meta":{"origin":9850,"position":5},"title":"Cyclospora Outbreak in the United States: Why Rapid Molecular Testing Matters for Gastrointestinal Pathogens","author":"Ashleigh Barlow","date":"July 17, 2026","format":false,"excerpt":"Fresh produce is one of the most common sources of Cyclospora outbreaks. Molecular testing helps laboratories rapidly identify Cyclospora cayetanensis alongside other gastrointestinal pathogens from a single specimen. Article Summary The United States is currently investigating a multistate outbreak of Cyclospora cayetanensis, a foodborne parasite that causes prolonged diarrheal illness.\u2026","rel":"","context":"In &quot;Food Safety&quot;","block_context":{"text":"Food Safety","link":"https:\/\/admin.acceleratingscience.com\/behindthebench\/food-safety\/"},"img":{"alt_text":"Magnified view of parasite contamination on fresh leafy greens, illustrating foodborne pathogen detection.","src":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Cyclospora-Image-6000x4480_Geoffrey-Cassell-scaled.jpeg?resize=350%2C200&ssl=1","width":350,"height":200,"srcset":"https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Cyclospora-Image-6000x4480_Geoffrey-Cassell-scaled.jpeg?resize=350%2C200&ssl=1 1x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Cyclospora-Image-6000x4480_Geoffrey-Cassell-scaled.jpeg?resize=525%2C300&ssl=1 1.5x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Cyclospora-Image-6000x4480_Geoffrey-Cassell-scaled.jpeg?resize=700%2C400&ssl=1 2x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Cyclospora-Image-6000x4480_Geoffrey-Cassell-scaled.jpeg?resize=1050%2C600&ssl=1 3x, https:\/\/i0.wp.com\/admin.acceleratingscience.com\/behindthebench\/wp-content\/uploads\/sites\/9\/2026\/07\/Cyclospora-Image-6000x4480_Geoffrey-Cassell-scaled.jpeg?resize=1400%2C800&ssl=1 4x"},"classes":[]}],"jetpack_sharing_enabled":true,"jetpack_featured_media_url":"","_links":{"self":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/9850","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/users\/120"}],"replies":[{"embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/comments?post=9850"}],"version-history":[{"count":0,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/posts\/9850\/revisions"}],"wp:attachment":[{"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/media?parent=9850"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/categories?post=9850"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/tags?post=9850"},{"taxonomy":"division","embeddable":true,"href":"https:\/\/www.thermofisher.com\/blog\/behindthebench\/wp-json\/wp\/v2\/division?post=9850"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}