Eliminate cell culture contamination for maximum sample protection is one of the main challenges facing cell culture laboratories. Whilst moist heat methods are ideal for rapid sterilization of wrapped instruments and other packaged items in hospital settings, dry heat sterilization has emerged as the technology of choice for the decontamination of equipment surfaces.
Spotless clean surfaces
Dry heat sterilization is a particularly efficient process when it comes to sterilizing moisture-sensitive items, such as cutting edge instruments, dry chemicals, oils, and glycerine. It’s the heat conducting properties of such equipment that allow them to be readily sterilized using dry heat processes. This technology is especially suitable for eliminating microorganisms and persistent fungal spores, both of which are a major concern in cell culture laboratories.
Modern carbon dioxide incubators are nowadays equipped with dry heat sterilization technology. Whenever decontamination is required, you can initiate a pre-programmed sterilization cycle and after just a few hours, your incubator will be completely sterilized and ready to use again. Advanced incubators also feature stainless steel and glass chambers, eliminating the need for corrosive copper salts or toxic chemicals removal.
When performing dry heat sterilization, there are certain variables you must take into account to comply with stringent standards established for the healthcare industry. Temperature and time are both important factors to consider as some spores need to be exposed to high heat for longer periods to ensure effective sterilization. Another factor to keep in mind is air distribution – hot air should flow uniformly over surfaces to reduce microorganism resistance by way of dehydration. The incubator load size and density is also critical as they affect the way surfaces are exposed to hot air.
Although various methods have been developed to destroy contaminants over the years, dry heat sterilization remains one of the most reliable and effective sterilization methods. There are a number of considerations to keep in mind when using this method, and selecting an incubator that is built with these in mind will go a long way in ensuring your cultures remain contaminant free.