{"id":4024,"date":"2015-03-05T17:03:24","date_gmt":"2015-03-05T22:03:24","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=4024"},"modified":"2016-04-21T19:21:22","modified_gmt":"2016-04-21T19:21:22","slug":"versatility-beyond-belief","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/cellculture\/versatility-beyond-belief\/","title":{"rendered":"Versatility Beyond Belief"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/cellculture\/wp-content\/uploads\/sites\/8\/2015\/03\/the_right_conditions_to_form_healthy_cultures.jpg\" height=\"282\" width=\"282\" style=\"float: right\" \/>During cell culture, it&rsquo;s crucial that a cell&rsquo;s artificial growth environment mirrors in vivo conditions as much as possible. By giving cells the right conditions, they have the best chance of forming healthy, dependable cultures for research purposes.&nbsp;<\/p>\n<p>In the past, you may have used a solid, non-porous surface, such as polystyrene for cell culture. This type of surface only allows gas and nutrient exchange to occur through the top of the cell, whereas <em>in vivo<\/em>, these processes occur from all directions.&nbsp;&nbsp;<\/p>\n<h2>Housing crisis<\/h2>\n<p>It is very difficult to mimic a cell&rsquo;s natural environment using a non-porous culture surface, but <a href=\"http:\/\/www.thermoscientific.com\/en\/product\/nunc-polycarbonate-membrane-inserts-multidishes.html\">Cell Culture Inserts<\/a>&nbsp;made with a porous polycarbonate membrane presents a viable solution. The inserts allow compounds to be transported from all directions, creating a more representative, three-dimensional environment. The pores also provide a means for cells to move toward certain chemical stimulus in the culture system mimicking chemotaxis <em>in vivo<\/em>.&nbsp;&nbsp;<\/p>\n<p>The versatile design of <a href=\"http:\/\/www.thermoscientific.com\/en\/product\/nunc-polycarbonate-membrane-inserts-multidishes.html\">Cell Culture Inserts<\/a>&nbsp;exposes cells in their entirety to growth media, while maintaining a separation barrier between the upper and lower compartments. This feature allows you to conduct various experiments such as co-culture where you can expose different cell types to a single environment simultaneously without the risk of cross contamination; something that is useful in understanding how different cells interact with the environment.<\/p>\n<h2>Endless possibilities<\/h2>\n<p>Normally, manipulating inserts halfway through an experiment can be messy, but the design of a Carrier Plate allows you to easily move inserts to a different hanging height within the wells or to a separate cell culture plate with different culture environmen<a><\/a>ts, opening up an array of possible experiments.<\/p>\n<p>While cell culture is an established technique, porous membranes have the potential to enhance and diversify its current applications. By better imitating an <em>in vivo<\/em> environment, cells are able to develop and respond more naturally than in a traditional monolayer culture.<\/p>\n<h2>More about the versatility of cell culture inserts<\/h2>\n<p>Want to learn more about the advantages of culturing cells on a porous membrane? Find the on demand Cell Culture Cafe Webinar, <a href=\"http:\/\/event.thermoscientific.com\/LP=2503\" target=\"_blank\"><em>Versatile is smart! The power behind culturing cells on a porous membrane <\/em><\/a>in the Thermo Scientific Cell Culture Community.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>During cell culture, it&rsquo;s crucial that a cell&rsquo;s artificial growth environment mirrors in vivo conditions as much as possible. By giving cells the right conditions, they have the best chance of forming healthy, dependable cultures for research purposes.&nbsp; In the past, you may have used a solid, non-porous surface, such as polystyrene for cell culture.<\/p>\n","protected":false},"author":130,"featured_media":4023,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[4],"tags":[15,16,17,18],"division":[],"class_list":{"0":"post-4024","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-general-cellculture","8":"tag-cell-culture-inserts","9":"tag-chemotaxis","10":"tag-co-culture","11":"tag-tissue-culture-inserts","12":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Versatility Beyond Belief - Advancing Cell Culture<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/cellculture\/versatility-beyond-belief\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Versatility Beyond Belief\" \/>\n<meta property=\"og:description\" content=\"During cell culture, it&rsquo;s crucial that a cell&rsquo;s artificial growth environment mirrors in vivo conditions as much as possible. 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