{"id":4249,"date":"2015-05-11T17:22:40","date_gmt":"2015-05-11T21:22:40","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=4249"},"modified":"2016-04-21T19:21:21","modified_gmt":"2016-04-21T19:21:21","slug":"can-you-reduce-the-edge-effect-in-your-cultures","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/cellculture\/can-you-reduce-the-edge-effect-in-your-cultures\/","title":{"rendered":"Can You Eliminate the Threats of Edge Effect During Long-term Incubation?"},"content":{"rendered":"<p>Evaporation during long-term incubation\u00a0can be\u00a0the enemy\u2014one of <em>many<\/em> enemies\u2014throughout the\u00a0cell culture process. When you use\u00a0a 96-well plate to culture\u00a0your cells, you hope for full use of your plate, resulting in viable, healthy cells in every well. However, when you lose the use of\u00a0the outer perimeter of your\u00a0plate in order to alleviate the devastating effects of evaporation, you have become a victim of\u00a0edge effect.<\/p>\n<p style=\"text-align: center\">Want to learn more? Explore our on-demand webinar:\u00a0<a href=\"http:\/\/event.thermoscientific.com\/LP=1789\" target=\"_blank\"><em>Take your Cell Based Assays to the Edge, in the Cell Culture Caf\u00e9<\/em><\/a><\/p>\n<p><strong>Threats of edge effect<\/strong><\/p>\n<p>If you evaluate\u00a0a standard 96-well plate, you\u2019ll observe differing rates of evaporation during long-term incubation, starting from the outer wells across the plate. A reduction in volume as low as 10 percent can\u00a0concentrate the media in each well enough to affect cells\u2019 physiology, causing variability that can compromise\u00a0the viability and integrity of your cells. Assay washing and aspiration can be another cause, leading to differing reagent concentrations in your plates.<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" style=\"margin: 2px;float: right\" title=\"Cell_Based_Assays_High_Throughput_Screening\" src=\"http:\/\/admin.acceleratingscience.com\/cellculture\/wp-content\/uploads\/sites\/8\/2015\/03\/tfs_1002_thmbnls-outerwell.jpg\" alt=\"hypoxia_bacteria_assay_reagents_cell_viability_apoptosis_evaporation_edge\" width=\"165\" height=\"165\" \/>You wouldn\u2019t be the only researcher to abandon the outer parameter\u00a0of your plate, which, on a 96-well plate, costs you over a third of the available space. A small sacrifice of that size per plate adds up when you consider how many plates your lab has in use at any moment.<\/p>\n<p><strong>Minimize edge effect<\/strong><\/p>\n<p>There are several ways to reduce edge effect and make full use of your 96-well plate:<\/p>\n<p>Evaluate your lab\u2019s workflow. You may find steps in your processes that expose plates to temperature gradients, airflow, or vibrations that disturb the settling of the cells into the plates and encourage uneven distribution.<\/p>\n<p>Cover your plates during incubation to reduce evaporation. For cell-based work, use plastic lids with condensation rings that allow gas exchange.<\/p>\n<p>You may also want to explore cultureware specifically designed to reduce edge effect. Learn\u00a0about our <a href=\"http:\/\/www.thermoscientific.com\/content\/dam\/tfs\/LPG\/LCD\/LCD%20Documents\/Catalogs%20&amp;%20Brochures\/Cell%20Culture%20Vessels%20and%20Microplates\/Microplates\/Cell%20Culture%20Microplates\/D01285~.pdf\" target=\"_blank\">Nunc Edge Plate<\/a>, whose special \u201cmoat\u201d-style reservoir minimizes evaporation to less than two percent during a seven-day incubation.<\/p>\n<p><strong>More on maximizing your assays<\/strong><\/p>\n<p>Hear from the experts! Explore\u00a0our on-demand webinar, <a href=\"http:\/\/event.thermoscientific.com\/LP=1789\" target=\"_blank\"><em><strong>Take your Cell Based Assays to the Edge,<\/strong><\/em><\/a><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Evaporation during long-term incubation\u00a0can be\u00a0the enemy\u2014one of many enemies\u2014throughout the\u00a0cell culture process. When you use\u00a0a 96-well plate to culture\u00a0your cells, you hope for full use of your plate, resulting in viable, healthy cells in every well. However, when you lose the use of\u00a0the outer perimeter of your\u00a0plate in order to alleviate the devastating effects of<\/p>\n","protected":false},"author":132,"featured_media":4254,"comment_status":"open","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_memberships_contains_paid_content":false,"footnotes":""},"categories":[4],"tags":[5,31,7,105,70,106,107],"division":[],"class_list":{"0":"post-4249","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-general-cellculture","8":"tag-cell-culture","9":"tag-cell-viability","10":"tag-cell-based-assays","11":"tag-high-throughput-screening","12":"tag-hypoxia","13":"tag-microplane","14":"tag-variation","15":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- This site is optimized with the Yoast SEO Premium plugin v27.8 (Yoast SEO v27.8) - https:\/\/yoast.com\/product\/yoast-seo-premium-wordpress\/ -->\n<title>Can You Eliminate the Threats of Edge Effect During Long-term Incubation? - Advancing Cell Culture<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/cellculture\/can-you-reduce-the-edge-effect-in-your-cultures\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Can You Eliminate the Threats of Edge Effect During Long-term Incubation?\" \/>\n<meta property=\"og:description\" content=\"Evaporation during long-term incubation\u00a0can be\u00a0the enemy\u2014one of many enemies\u2014throughout the\u00a0cell culture process. 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