{"id":1355,"date":"2020-05-13T14:29:03","date_gmt":"2020-05-13T14:29:03","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/connectedlab\/?p=1355"},"modified":"2024-08-27T09:24:16","modified_gmt":"2024-08-27T09:24:16","slug":"managing-ngs-workflows-with-lims","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/connectedlab\/managing-ngs-workflows-with-lims\/","title":{"rendered":"Managing NGS Workflows with LIMS"},"content":{"rendered":"<p>Anyone in a lab running next generation sequencing (NGS) experiments knows what a challenge it is to keep track of the myriad samples, libraries, adapters, reagents, instruments and associated data.\u00a0 Trying to manually track these with spreadsheets and notebooks can prove to be a frustrating and erratic experience.\u00a0 We are scientists just like you, and we have experienced these same frustrations.\u00a0 As a result, we developed a reliable way to trace samples and experiments; an easy way to access the details of the reagents consumed, the instrumentation used, and the scientists who performed the experiments.\u00a0<img decoding=\"async\" src=\"https:\/\/www.thermofisher.com\/blog\/connectedlab\/wp-content\/uploads\/sites\/14\/2020\/05\/deeper-insights-1.png\" \/><\/p>\n<h2>Producing High-Quality Libraries<\/h2>\n<p>NGS is an essential tool for researchers, and the quality of library preparation is a critical success factor.\u00a0 High-quality libraries generate high-quality results.\u00a0 With the many steps involved to produce high-quality libraries, it is imperative to track where in the workflow these samples are and minimize potential error and rework, all without a cumbersome amount of administrative overhead.\u00a0 The latest advances to the Library Prep capabilities in the <a href=\"https:\/\/www.thermofisher.com\/blog\/connectedlab\/new-core-lims-software-apps-support-ngs-and-biobanking-data-management\/\">NGS Workflow Solution<\/a> for Thermo Scientific\u2122 Core LIMS\u2122 software streamlines data capture, linking sample metadata to assay data, and tracks sample progression through the workflow steps in real time.\u00a0 Specifically, researchers can now:<\/p>\n<ul>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Easily visualize library prep plates<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Add barcodes or adapters to samples using drag and drop capabilities<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Cherry pick samples from multiple containers for processing in parallel<\/li>\n<\/ul>\n<p><img decoding=\"async\" src=\"https:\/\/kapost-files-prod.s3.amazonaws.com\/kapost\/5d1ccc4b5e7eb0000400096a\/studio\/content\/5ebbe9e65e7030015d5f4855\/html_bodies\/1589373472-709d87cf-de49-47cf-a1cb-ba2c93ab3c7d\/Library%20Prep%20Shiny%20GIF%20project.gif\" \/><\/p>\n<h2>Multiplexing and Pooling Libraries<\/h2>\n<p>Maximizing the cost-effectiveness of sequencing is another major concern for many labs.\u00a0 One of the best ways to maximize productivity around sequencing is to take full advantage of the capacity of a sequencing platform by multiplexing and pooling libraries.\u00a0 This can come with its own set of pitfalls.\u00a0 Normalizing the libraries so that each is equally represented in the pool is critical for successful data outcomes.\u00a0 And equally significant is ensuring that no index adapter or library barcode is duplicated within a pool, otherwise differentiating the data of multiplexed libraries would be impossible.\u00a0 The enhancements to the pooling capabilities with this the NGS Workflow solution now enable researchers to:<\/p>\n<ul>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Normalize libraries with automatically calculated dilution instructions<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Pool libraries using drag-and-drop functionality to maximize sequencing capacity<\/li>\n<li>\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0\u00a0 Immediately spot index clashes<\/li>\n<\/ul>\n<p><img decoding=\"async\" src=\"https:\/\/kapost-files-prod.s3.amazonaws.com\/kapost\/5d1ccc4b5e7eb0000400096a\/studio\/content\/5ebbe9e65e7030015d5f4855\/html_bodies\/1589373515-192886f7-7ebd-4b16-bf3c-ba0dde999432\/Library%20Pooling%20Shiny%20GIF%20project.gif\" \/><\/p>\n<h2>Pre-configured Solution for NGS<\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/uk\/en\/home\/digital-solutions\/lab-informatics\/lab-information-management-systems-lims\/solutions\/core.html\">Core LIMS software<\/a> provides a streamlined, pre-configured solution for NGS to track and manage data collection, from sample prep through sequencing, regardless of instrumentation.\u00a0 Samples progress through the NGS workflow process from one step to the next, automatically tracking chain of custody, parent\/child relationships, and all metadata associated with each experiment.\u00a0 Integrated dashboards also provide insight into lab productivity in order to quickly spot any bottlenecks.\u00a0 NGS supports a broad range of applications and disciplines, and across these is a common need to collect, access and report data.\u00a0 The NGS Workflow Solution facilitates all aspects of data collection, access and reporting.\u00a0 It is designed to be intuitive and simple to use, thereby minimizing implementation timelines and maximizing productivity.<\/p>\n<p>Read our <a href=\"https:\/\/assets.thermofisher.com\/TFS-Assets\/DSD\/brochures\/pfs-genomics-ngs-all-vendors-brochure.pdf\" target=\"_blank\" rel=\"noopener\">Accelerating genomic studies with Platform for Science software brochure<\/a> on our NGS Workflow Solutions to learn more about how we can support your genomic workflows.<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Anyone in a lab running next generation sequencing (NGS) experiments knows what a challenge it is to keep track of the myriad samples, libraries, adapters, reagents, instruments and associated data.\u00a0 Trying to manually track these with spreadsheets and notebooks can prove to be a frustrating and erratic experience.\u00a0 We are scientists just like you, and<\/p>\n","protected":false},"author":1245,"featured_media":1361,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[107],"tags":[1044,95,162,96,97,98],"division":[],"class_list":{"0":"post-1355","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-genomics","8":"tag-core-lims","9":"tag-lab-data-management","10":"tag-lab-operations","11":"tag-lims","12":"tag-next-generation-sequencing","13":"tag-ngs","14":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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