{"id":2313,"date":"2014-02-14T04:00:25","date_gmt":"2014-02-14T09:00:25","guid":{"rendered":"http:\/\/admin.acceleratingscience.com\/?p=2313"},"modified":"2016-04-26T17:20:12","modified_gmt":"2016-04-26T17:20:12","slug":"a-rapid-approach-for-the-screening-of-human-serum-albumin-binders-in-radix-astragali","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/food\/a-rapid-approach-for-the-screening-of-human-serum-albumin-binders-in-radix-astragali\/","title":{"rendered":"A Rapid Approach for the Screening of Human Serum Albumin Binders in Radix Astragali"},"content":{"rendered":"

\"\"In Asia, the medicinal herb and dietary supplement Radix Astragali<\/a> enjoys a solid and longstanding reputation for its antioxidative, anti inflammatory, antitumoral, antiviral, immunostimulant, and hepatoprotective <\/a>properties.1,2,3,4<\/sup> While previous researchers identified isoflavonoids and astragalosides as the major active metabolites of the herb, inadequate information exists on its biochemical structure. \u00a0<\/span> Recently, Zhang et al.5<\/sup> used magnetic solid phase \ufb01shing and off-line two-dimensional high-performance liquid chromatography-diode array detection multiple stage mass spectrometry (2D HPLC\u2013DAD\u2013MSn) with size exclusion chromatography (SEC) followed by radical probe (RP) mass spectrometry (Thermo Scientific) to complete the first systematic analysis of human serum albumin <\/a>(HSA) binders in Radix Astragali. \u00a0This examination arose as part of a continuing project to create rapid screening protocols for the identification of bioactive compounds in natural products. Using this method, the researchers identified or inferred 47 biologically active compounds in Radix Astragali<\/span>, including 34 isoflavonoids<\/a> and 13 astragalosides<\/a>. The team made tentative identifications based on solid factors, including retention time, molecular weight, comparison with diagnostic fragment ions, and ultraviolet signatures. \u00a0In general, isoflavones (compounds 1-4, 6-18, 21, 23, 25, 27, 33, 34, 38) presented a maximum absorbance near 260 nm and a second peak between 290 and 320 nm. Astragalosides (compounds 29, 30, 32, 35-37, 39, 42-47) demonstrated weak ultraviolet peaks at 280 nm. \u00a0Both isoflavans and pterocarpans (compounds 5, 19, 20, 22, 24, 26, 28, 31, 40, 41) showed maximum absorption at 280 nm. \u00a0The researchers explained that instances of higher molecular weight most likely indicated glycosylation. Overall, Zhang et al. found their method provides a sensitive, reliable alternative that offers effective, rapid bioprofiling of even complex mixtures in a decidedly convenient manner. The protocol may be especially useful for researchers working with compounds that contain overlapping or low abundance components or who seek an approach that does not require separate purification steps. References <\/strong> <\/p>\n

    \n
  1. Chu, C. et al. (2010). \u2018Characterization of novel astragaloside malonates from Radix Astragali by HPLC with ESI quadrupole TOF MS<\/a>.\u2019 Journal of Separation Science, 33, 570\u2013581.<\/li>\n
  2. Huang, X. et al. (2009) \u2018Studies on principle components and antioxidant activity of different Radix Astragali samples using high-performance liquid chromatography\/electrospray ionization multiplestage tandem mass spectrometry<\/a>.\u2019 Talanta, 78, 1090\u20131101.<\/li>\n
  3. Lau, K. M. et al (2012). \u2018Synergistic interaction between Astragali Radix and Rehmanniae Radix in a Chinese herbal formula to promote diabetic wound healing<\/a>\u2019. Journal of Ethnopharmacology, 141, 250\u2013256.<\/li>\n
  4. Qi, L. W. et al. (2008). \u2018Qualitative and quantitative analysis of Radix Astragali products by fast high-performance liquid chromatography-diode array detection coupled with time-of-\ufb02ight mass\u00a0spectrometry through dynaminc adjustment of fragment voltage<\/a>.\u2019 Journal of Chromatography A, 1203, 27\u201335.<\/li>\n
  5. Zhang, Y. et al. (2014) \u2018Integration of magnetic solid phase \ufb01shing and off-line two-dimensional high-performance liquid chromatography\u2013diode array detector\u2013mass spectrometry for screening and identi\ufb01cation of human serum albumin binders from Radix Astragali<\/a>.\u2019 Food Chemistry, 146, 56\u201364.<\/li>\n<\/ol>\n","protected":false},"excerpt":{"rendered":"

    In Asia, the medicinal herb and dietary supplement Radix Astragali enjoys a solid and longstanding reputation for its antioxidative, anti inflammatory, antitumoral, antiviral, immunostimulant, and hepatoprotective properties.1,2,3,4 While previous researchers identified isoflavonoids and astragalosides as the major active metabolites of the herb, inadequate information exists on its biochemical structure. \u00a0 Recently, Zhang et al.5 used<\/p>\n","protected":false},"author":16,"featured_media":2312,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_publicize_message":"","jetpack_publicize_feature_enabled":true,"jetpack_social_post_already_shared":true,"jetpack_social_options":{"image_generator_settings":{"template":"highway","default_image_id":0,"font":"","enabled":false},"version":2},"jetpack_post_was_ever_published":false},"categories":[17],"tags":[],"division":[],"class_list":{"0":"post-2313","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-quality-control-nutrition","8":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\nA Rapid Approach for the Screening of Human Serum Albumin Binders in Radix Astragali - Examining Food<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/food\/a-rapid-approach-for-the-screening-of-human-serum-albumin-binders-in-radix-astragali\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"A Rapid Approach for the Screening of Human Serum Albumin Binders in Radix Astragali\" \/>\n<meta property=\"og:description\" content=\"In Asia, the medicinal herb and dietary supplement Radix Astragali enjoys a solid and longstanding reputation for its antioxidative, anti inflammatory, antitumoral, antiviral, immunostimulant, and hepatoprotective properties.1,2,3,4 While previous researchers identified isoflavonoids and astragalosides as the major active metabolites of the herb, inadequate information exists on its biochemical structure. \u00a0 Recently, Zhang et al.5 used\" \/>\n<meta property=\"og:url\" content=\"https:\/\/www.thermofisher.com\/blog\/food\/a-rapid-approach-for-the-screening-of-human-serum-albumin-binders-in-radix-astragali\/\" \/>\n<meta property=\"og:site_name\" content=\"Examining Food\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/thermofisher\" \/>\n<meta property=\"article:published_time\" content=\"2014-02-14T09:00:25+00:00\" \/>\n<meta property=\"article:modified_time\" content=\"2016-04-26T17:20:12+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/admin.acceleratingscience.com\/food\/wp-content\/uploads\/sites\/5\/2014\/02\/radix_astragali_200_across.jpg\" \/>\n\t<meta property=\"og:image:width\" content=\"200\" \/>\n\t<meta property=\"og:image:height\" content=\"200\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/jpeg\" \/>\n<meta name=\"author\" content=\"Melissa J. 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