{"id":10024,"date":"2021-09-17T09:09:27","date_gmt":"2021-09-17T00:09:27","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=10024"},"modified":"2021-10-01T10:14:18","modified_gmt":"2021-10-01T01:14:18","slug":"3ivtplusreagentkit_protocol_ma_gsd_mkt_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/3ivtplusreagentkit_protocol_ma_gsd_mkt_1\/","title":{"rendered":"3\u2019IVT Plus Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb"},"content":{"rendered":"

\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u4f7f\u7528\u3057\u305f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306f\u73fe\u5728\u3082\u3063\u3068\u3082\u30dd\u30d4\u30e5\u30e9\u30fc\u306a\u65b9\u6cd5\u3067\u3001\u767a\u73fe\u6bd4\u8f03\u7814\u7a76\u3084\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u63a2\u7d22\u306b\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u5b9f\u7e3e\u304c\u3042\u308a\u307e\u3059\u3002\u8fd1\u5e74\u3082\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u3084IncRNA\u89e3\u6790\u3092\u4e2d\u5fc3\u306b\u30a2\u30ec\u30a4\u306f\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304a\u308a\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306e\u4e2d\u5fc3\u30d7\u30ec\u30fc\u30e4\u30fc\u3067\u3042\u308b\u3068\u3044\u3048\u307e\u3059\u3002
\n\u4eca\u56de\u306e\u30d6\u30ed\u30b0\u3067\u306f\u3001GeneChip\u2122 3′ IVT PLUS Reagent Kit \u306e\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306b\u95a2\u3059\u308b\u624b\u5f15\u304d\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002GeneChip\u2122 3′ IVT PLUS Reagent Kit \u306f\u3001\u7dcfRNA\u91cf\u308f\u305a\u304b50ng\u30921\u56de\u5897\u5e45\u3059\u308b\u3060\u3051\u3067\u691c\u51fa\u306b\u9032\u3081\u308b\u3053\u3068\u304c\u3067\u304d\u3001GeneChip 3′ IVT Express Kit\uff083′ IVT Express Kit\uff09\u3092\u7528\u3044\u308b\u3053\u3068\u3067\u3001\u524d\u30c7\u30fc\u30bf\u3092\u640d\u306a\u3046\u3053\u3068\u306a\u304f\u9ad8\u54c1\u8cea\u30c7\u30fc\u30bf\u3092\u7d99\u7d9a\u7684\u306b\u53d6\u5f97\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u53c2\u8003\u306b\u3001\u82e5\u3044\u7814\u7a76\u8005\u3055\u307e\u65b9\u306b\u3082\u6539\u3081\u3066\u3001GeneChip WT\u30a2\u30ec\u30a4\u306e\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u306b\u95a2\u3059\u308b\u3042\u308c\u3053\u308c\u3092\u5e83\u304f\u77e5\u3063\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u5bfe\u8c61\u88fd\u54c1\uff1aApplied Biosystems\u2122 GeneChip\u2122 3’IVT Plus Reagent Kit<\/p>\n

\u5bfe\u8c61\u88fd\u54c1\u756a\u53f7\uff1a902415\uff0810\u53cd\u5fdc\u5206\uff09\u3001902416\uff0830\u53cd\u5fdc\u5206\uff09<\/p>\n

\u516c\u5f0f\u30e6\u30fc\u30b6\u30fc\u30ac\u30a4\u30c9<\/a>\u3068\u4f75\u305b\u3066\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u203b3’IVT Plus Reagent Kit\u306b\u306f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306f\u4ed8\u5c5e\u3057\u307e\u305b\u3093\u30023’IVT Plus Reagent Kit\u3068\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u306e\u30bb\u30c3\u30c8\u54c1\u3001\u3082\u3057\u304f\u306f3’IVT Plus Reagent Kit\u306b\u5bfe\u5fdc\u3059\u308b\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u3054\u8cfc\u5165\u304f\u3060\u3055\u3044\u3002\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3068\u8a66\u85ac\u306e\u9078\u629e\u306b\u3064\u3044\u3066\u306fExpression Microarray Reagent Guide<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u3053\u306e\u30ad\u30c3\u30c8\u306e\u307b\u304b\u306b\u3001\u3042\u3089\u304b\u3058\u3081\u3054\u7528\u610f\u3044\u305f\u3060\u304f\u8a66\u85ac<\/h2>\n

\u30fb100%\u30a8\u30bf\u30ce\u30fc\u30eb
\n\u30fbApplied Biosystems\u2122 GeneChip\u2122 Hybridizatoin, Wash, and Stain kit \uff08\u88fd\u54c1\u756a\u53f7\u3000900720\uff0830\u53cd\u5fdc\u5206\uff09\uff09<\/p>\n

\u30a2\u30c3\u30bb\u30a4\u306b\u4f7f\u7528\u3059\u308bRNA\u91cf<\/h2>\n

IMPORTANT<\/strong>\uff1a
\n<\/strong>Total RNA\u5bb9\u91cf\u306f5 \u03bcL\u4ee5\u4e0b\u306b\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\uff08poly-A RNA controls\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u306f3 \u03bcL\u4ee5\u4e0b\uff09\u3002<\/p>\n

\"\"
\n\"\"<\/p>\n

TIP\uff1a<\/strong>\u8a66\u85ac\u8abf\u88fd\u306b\u95a2\u3057\u3066
\n<\/strong>\u30fb\u30ad\u30c3\u30c8\u306e\u51cd\u7d50\u878d\u89e3\u306f3\u56de\u4ee5\u4e0b\u3067\u4f7f\u7528\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\u30fb\u9175\u7d20\u985e\u306f\u7a4f\u3084\u304b\u306b\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306b\u304b\u3051\u3066\u6df7\u5408\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30d0\u30c3\u30d5\u30a1\u30fc\u985e\u306f\u6c88\u6bbf\u7269\u304c\u306a\u304f\u306a\u308b\u307e\u3067\u3001\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306b\u304b\u3051\u3066\u3088\u304f\u6df7\u5408\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\u30fb<\/strong>\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3084\u30b5\u30f3\u30d7\u30eb\u306f\u7a4f\u3084\u304b\u306b\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306b\u304b\u3051\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u305f\u5f8c\u306b\u7d20\u65e9\u304f\u30b9\u30d4\u30f3\u30c0\u30a6\u30f3\u3057\u3066\u3001\u6eb6\u6db2\u3092\u96c6\u3081\u3066\u304f\u3060\u3055\u3044\u3002
\n\u30fb\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306f\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u3088\u308b\u30ed\u30b9\u306b\u5099\u3048\u308b\u305f\u3081\u306b10%\u7a0b\u5ea6\u904e\u5270\u306b\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\u30fb\u53cd\u5fdc\u5f8c\u306f\u30b5\u30f3\u30d7\u30eb\u30c1\u30e5\u30fc\u30d6\u3092\u7c21\u6f54\u306b\u30b9\u30d4\u30f3\u30c0\u30a6\u30f3\u3057\u3001\u6b21\u306e\u30b9\u30c6\u30c3\u30d7\u306b\u9032\u3080\u524d\u306b\u306f\u6c37\u4e0a\u306b\u7f6e\u3044\u3066\u304f\u3060\u3055\u3044\u3002
\n\u30fb\u9175\u7d20\u3092\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u306b\u52a0\u3048\u308b\u306e\u306f\u53cd\u5fdc\u3092\u884c\u3046\u76f4\u524d\u306b\u884c\u3046\u3088\u3046\u306b\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

Section 1\uff1aPoly-A RNA Controls\u3001Total RNA\u306e\u6e96\u5099<\/h2>\n

1. \u4f7f\u7528\u3059\u308bTotal RNA\u91cf\u306b\u5fdc\u3058\u3066\u3001Poly-A RNA Controls\u3092\u5e0c\u91c8\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\"\"
\nTIP\uff1a<\/strong>\u5e0c\u91c8\u3059\u308b\u6642\u306f\u6b63\u78ba\u6027\u3084\u4e00\u8cab\u6027\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306b2 \u03bcL\u672a\u6e80\u306e\u5bb9\u91cf\u3067\u306e\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u306f\u907f\u3051\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

2. \u5e0c\u91c8\u3057\u305fPoly-A RNA Controls\u3068Total RNA\u30b5\u30f3\u30d7\u30eb\u3092\u6df7\u305c\u307e\u3059\u3002\u5408\u308f\u305b\u30665 \u03bcL\u306b\u306a\u308b\u3088\u3046\u306b\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\"\"<\/p>\n

Section 2\uff1aFirst-Strand cDNA\u5408\u6210<\/h2>\n

1. \u6c37\u4e0a\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002
\n\"\"<\/p>\n

2. \u6c37\u4e0a\u3067\u53cd\u5fdc\u306b\u4f7f\u7528\u3059\u308b\u30c1\u30e5\u30fc\u30d6\u3078\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b95 \u03bcL\u3092\u5206\u6ce8\u3057\u3001Table 2\u3067\u4f5c\u6210\u3057\u305fTotal RNA\/Poly-A Control Mixture\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 10 mL\uff09\u3002
\n3. First-Strand cDNA Synthesis program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306642\u2103\u30672\u6642\u9593\u30014\u2103\u30672\u5206\u4ee5\u4e0a\u306e\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002
\n4. \u53cd\u5fdc\u7d42\u4e86\u5f8c\u306f\u901f\u3084\u304b\u306bSecond-Strand cDNA\u5408\u6210\u306b\u9032\u307f\u307e\u3059\u3002<\/p>\n

Section 3\uff1aSecond-Strand cDNA\u5408\u6210<\/h2>\n

1. \u6c37\u4e0a\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002
\nNOTE\uff1a<\/strong>\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u3066\u3044\u308b\u9593\u306b\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u30d6\u30ed\u30c3\u30af\u6e29\u5ea6\u309216\u2103\u306b\u3042\u3089\u304b\u3058\u3081\u51b7\u5374\u3057\u3066\u304a\u304d\u307e\u3059\u3002\u307e\u305f\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u3075\u305f\u306b\u3064\u3044\u3066\u306f\u3001\u52a0\u71b1\u3092\u6b62\u3081\u308b\u304b\u3001\u3075\u305f\u306e\u6e29\u5ea6\u5236\u5fa1\u304c\u3067\u304d\u306a\u3044\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u5834\u5408\u306f\u3075\u305f\u3092\u958b\u3051\u305f\u307e\u307e\u3067\u53cd\u5fdc\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\"\"<\/p>\n

2. \u6c37\u4e0a\u3067\u53cd\u5fdc\u306b\u4f7f\u7528\u3059\u308bFirst Strand cDNA\u30b5\u30f3\u30d7\u30eb10 \u03bcL\u306b\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b920 \u03bcL\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 30 \u03bcL\uff09\u3002
\n3. Second-Strand cDNA Synthesis program\uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306616\u2103\u30671\u6642\u9593\u300165\u2103\u306710\u5206\u30014\u2103\u30672\u5206\u306e\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u3002
\n4. \u53cd\u5fdc\u7d42\u4e86\u5f8c\u306f\u901f\u3084\u304b\u306bIn Vitro Transcription\u306b\u9032\u307f\u307e\u3059\u3002<\/p>\n

Section 4\uff1aIn Vitro Transcription\u306b\u3088\u308bLabeled cRNA\u5408\u6210<\/h2>\n

1. \u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u8abf\u88fd\u6642\u3001Second-Strand cDNA\u30b5\u30f3\u30d7\u30eb\u306f\u5ba4\u6e29\u306b5\u5206\u4ee5\u4e0a\u7f6e\u304d\u307e\u3059\u3002
\n2. \u5ba4\u6e29\u3067\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002
\nNOTE<\/strong>\uff1a<\/strong>IVT Buffer\u309210\u5206\u4ee5\u4e0a\u5ba4\u6e29\u306b\u7f6e\u3044\u3066\u304b\u3089\u3001\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b9\u3092\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002
\n\"\"
\n3. \u5ba4\u6e29\u3067\u5404Second-Strand cDNA \u30b5\u30f3\u30d7\u30eb30 \u03bcL\u306b\u30de\u30b9\u30bf\u30fc\u30df\u30c3\u30af\u30b930 \u03bcL\u3092\u52a0\u3048\u307e\u3059\uff08Total volume 60 \u03bcL\uff09\u3002
\n4. In Vitro Transcription cRNA Synthesis program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\uff08\u3075\u305f\u306e\u6e29\u5ea6\u309240\u2103\u3001\u307e\u305f\u306f50\u2103\u306b\u8a2d\u5b9a\uff09\u306b\u306640\u2103\u30674\uff5e16\u6642\u9593\uff08Total RNA\u304c250\uff5e500 ng\u306e\u5834\u5408\u306f4\u6642\u9593\u300150\uff5e250 ng\u306e\u5834\u5408\u306f16\u6642\u9593\uff09\u884c\u3063\u3066\u304f\u3060\u3055\u3044\u3002\u3075\u305f\u306e\u6e29\u5ea6\u5236\u5fa1\u304c\u3067\u304d\u306a\u3044\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306e\u5834\u5408\u306f\u3001Oven\u3092\u5229\u7528\u3057\u306640\u2103\u3067\u53cd\u5fdc\u3092\u884c\u3044\u307e\u3059\u30025. \u53cd\u5fdc\u7d42\u4e86\u5f8c\u306fcRNA\u7cbe\u88fd\u306b\u9032\u3080\u304b\u3001\u901f\u3084\u304b\u306b\u51b7\u51cd\u4fdd\u5b58\u3057\u307e\u3059\u3002
\n5. \u53cd\u5fdc\u7d42\u4e86\u5f8c\u306fcRNA\u7cbe\u88fd\u306b\u9032\u3080\u304b\u3001\u901f\u3084\u304b\u306b\u51b7\u51cd\u4fdd\u5b58\u3057\u307e\u3059\u3002
\nTIP<\/strong>\uff1a<\/strong>STOPPING POINT\uff5ecRNA\u30b5\u30f3\u30d7\u30eb\u306f-20\u2103\u3067\u4e00\u6669\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002<\/p>\n

Section 5\uff1acRNA\u7cbe\u88fd<\/h2>\n

cRNA<\/strong>\u7cbe\u88fd\u3092\u59cb\u3081\u308b\u524d\u306b\uff1a
\n<\/strong>\u30fb\u5c11\u306a\u304f\u3068\u308210\u5206\u9593\u306fNuclease-free Water\u309265\u2103\u306b\u3042\u3089\u304b\u3058\u3081\u52a0\u6e29\u3057\u3066\u304a\u304d\u307e\u3059\u3002
\n\u30fb\u7cbe\u88fd\u30d3\u30fc\u30ba\u306f\u4f7f\u7528\u524d\u306b\u3088\u304f\u6df7\u5408\u3057\u3066\u304a\u304d\u307e\u3059\u3002\u9069\u5f53\u91cf\u3092\u5206\u5272\u3057\u3066\u5ba4\u6e29\u3067\u4fdd\u6301\u3057\u307e\u3059\u3002\u5404\u53cd\u5fdc\u30b5\u30f3\u30d7\u30eb\u306b\u5bfe\u3057\u3066100 \u03bcL+10%\u904e\u5270\u91cf\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002
\n\u30fb80%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2\u306f\u5fc5\u305a\u7528\u6642\u8abf\u88fd\u3057\u307e\u3059\u3002\u5404\u53cd\u5fdc\u30b5\u30f3\u30d7\u30eb\u5bfe\u3057\u3066600 \u03bcL+10%\u904e\u5270\u91cf\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002
\nNOTE<\/strong>\uff1a<\/strong>\u3059\u3079\u3066\u306e\u5de5\u7a0b\u3092\u5ba4\u6e29\u3067\u884c\u3044\u307e\u3059\u3002<\/p>\n

\u7cbe\u88fd\u30d3\u30fc\u30ba\u3078\u306ecRNA\u7d50\u5408<\/p>\n

    \n
  1. \u7cbe\u88fd\u30d3\u30fc\u30ba\u3092\u518d\u61f8\u6fc1\u3059\u308b\u305f\u3081\u306b\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u3002\u7cbe\u88fd\u30d3\u30fc\u30ba100 \u03bcL\u3092\u4e38\u5e95\u30a6\u30a7\u30eb\u30d7\u30ec\u30fc\u30c8\u306e\u672a\u4f7f\u7528\u30a6\u30a7\u30eb\u306b\u79fb\u3057\u307e\u3059\u3002\u7d9a\u3044\u3066\u30d3\u30fc\u30ba\u306e\u5165\u3063\u305f\u30a6\u30a7\u30eb\u306b\u5404cRNA\u30b5\u30f3\u30d7\u30eb60 \u03bcL\u3092\u52a0\u3048\u3066\u3001\u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u3067\u6df7\u5408\u3057\u307e\u3059\u3002<\/li>\n
  2. \u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u309210\u56de\u884c\u3063\u3066\u3088\u304f\u6df7\u5408\u3057\u305f\u5f8c\u300110\u5206\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n
  3. \u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u96c6\u3081\u308b\u305f\u3081\u306b\u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u79fb\u52d5\u3055\u305b\u3066\u30015\u5206\u9593\u7a0b\u5ea6\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n
  4. \u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u5206\u6563\u3055\u305b\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u3066\u4e0a\u6e05\u3092\u56de\u53ce\u3057\u3066\u5ec3\u68c4\u3057\u307e\u3059\u3002\u30d7\u30ec\u30fc\u30c8\u306f\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u9759\u7f6e\u3057\u3066\u304a\u304d\u307e\u3059\u3002<\/li>\n<\/ol>\n

    \u7cbe\u88fd\u30d3\u30fc\u30ba\u306e\u6d17\u6d44<\/p>\n

      \n
    1. \u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u3067\u5404\u30a6\u30a7\u30eb\u306b80%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2200 \u03bcL\u3092\u52a0\u3048\u306630\u79d2\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n
    2. \u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u5206\u6563\u3055\u305b\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u3066\u3086\u3063\u304f\u308a\u306880%\u30a8\u30bf\u30ce\u30fc\u30eb\u6d17\u6d44\u6db2\u3092\u56de\u53ce\u3057\u3066\u5ec3\u68c4\u3057\u307e\u3059\u3002<\/li>\n
    3. \u30b9\u30c6\u30c3\u30d7A\u3068\u30b9\u30c6\u30c3\u30d7B\u3092\u7e70\u308a\u8fd4\u3057\u3001\u8a083\u56de\u306e\u6d17\u6d44\u3092\u884c\u3044\u307e\u3059\u3002\u6700\u7d42\u6d17\u6d44\u6db2\u306f\u5b8c\u5168\u306b\u9664\u53bb\u3057\u307e\u3059\u3002<\/li>\n
    4. \u6db2\u4f53\u304c\u898b\u3048\u306a\u304f\u306a\u308b\u307e\u30675\u5206\u9593\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u3067\u98a8\u4e7e\u3055\u305b\u307e\u3059\u3002\u72b6\u6cc1\u306b\u3088\u3063\u3066\u306f\u3055\u3089\u306b\u6642\u9593\u3092\u8981\u3059\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u30d3\u30fc\u30ba\u3092\u4e7e\u304b\u3057\u3059\u304e\u306a\u3044\u3088\u3046\u306b\u6ce8\u610f\u3057\u307e\u3059\u3002<\/li>\n<\/ol>\n

      cRNA\u306e\u6eb6\u96e2<\/p>\n

        \n
      1. \u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u304b\u3089\u4e0b\u308d\u3057\u307e\u3059\u3002\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u3001\u3042\u3089\u304b\u3058\u308165\u2103\u306b\u52a0\u6e29\u3057\u3066\u304a\u3044\u305fNuclease-free Water 27 \u03bcL\u3092\u52a0\u3048\u30661\u5206\u9593\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n
      2. \u30d4\u30da\u30c3\u30c6\u30a3\u30f3\u30b0\u64cd\u4f5c\u309210\u56de\u884c\u3063\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u3002<\/li>\n
      3. \u78c1\u6c17\u30d3\u30fc\u30ba\u3092\u96c6\u3081\u308b\u305f\u3081\u306b\u30d7\u30ec\u30fc\u30c8\u3092\u30de\u30b0\u30cd\u30c3\u30c8\u30b9\u30bf\u30f3\u30c9\u4e0a\u306b\u79fb\u52d5\u3055\u305b\u3066\u30015\u5206\u9593\u7a0b\u5ea6\u9759\u7f6e\u3057\u307e\u3059\u3002<\/li>\n
      4. \u6eb6\u96e2\u3055\u308c\u305fcRNA\u304c\u542b\u3093\u3060\u4e0a\u6e05\u3092nuclease-free\u306e\u30c1\u30e5\u30fc\u30d6\u306b\u79fb\u3057\u307e\u3059\u3002<\/li>\n
      5. \u7cbe\u88fd\u3055\u308c\u305fcRNA\u3092\u6c37\u4e0a\u306b\u7f6e\u304d\u3001\u5b9a\u91cf\u306b\u9032\u307f\u307e\u3059\u3002<\/li>\n<\/ol>\n

        TIP<\/strong>\uff1a<\/strong>STOPPING POINT\uff5e\u7cbe\u88fd\u3055\u308c\u305fcRNA\u30b5\u30f3\u30d7\u30eb\u306f-20\u2103\u3067\u4e00\u6669\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002\u9577\u671f\u4fdd\u5b58\u3059\u308b\u5834\u5408\u306f\u3001-80\u2103\u3067\u4fdd\u5b58\u3057\u307e\u3059\u3002<\/p>\n

        Section 6\uff1acRNA\u53ce\u91cf\u306e\u78ba\u8a8d<\/h2>\n

        Thermo Scientific\u2122 NanoDrop\u2122 Spectrophotometer\u306a\u3069\u306e\u6e2c\u5b9a\u6a5f\u5668\u3067260 nm\u306e\u5438\u5149\u5ea6\u6e2c\u5b9a\u3092\u884c\u3063\u3066\u3001cRNA\u30b5\u30f3\u30d7\u30eb\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3057\u307e\u3059\u3002\u6b21\u306e\u5de5\u7a0b\u306b\u9032\u3080\u305f\u3081\u306b\u306f\u30017.5\uff5e15 \u03bcg\u306ecRNA\u304c\u5fc5\u8981\u3067\u3059\uff08Table 6\u53c2\u7167\uff09\u3002
        \n\u203b\u30d3\u30fc\u30ba\u7cbe\u88fd\u5f8c\u306e\u6fc3\u5ea6\u6e2c\u5b9a\u306b\u3088\u308b\u6db2\u91cf\u30ed\u30b9\u306a\u3069\u3092\u8003\u616e\u3059\u308b\u3068\u3001\u5fc5\u8981\u91cf\u3092\u78ba\u4fdd\u3059\u308b\u305f\u3081\u306b\u3001\u5438\u5149\u5ea6\u6e2c\u5b9a\u6642\u306ecRNA\u6fc3\u5ea6\u306f\u76ee\u5b89\u3068\u3057\u3066469\uff5e521 ng\/\u03bcL\u4ee5\u4e0a\u5fc5\u8981\u3067\u3059\uff08Table 6\u53c2\u7167\uff09\u3002<\/p>\n

        Section 7\uff1aLabeled cDNA\u306e\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u5316<\/h2>\n

        1. Array\u306e\u30bf\u30a4\u30d7\u306b\u5408\u308f\u305b\u3001\u6c37\u4e0a\u3067Nuclease-free Water\u3092\u4f7f\u3063\u3066\u3001Labeled cRNA\u3092\u5404\u5bb9\u91cf\u306b\u8abf\u6574\u3057\u3001\u5fc5\u8981\u91cf\u306e3\u2019 Fragmentation Buffer\u3092\u52a0\u3048\u3066\u304f\u3060\u3055\u3044\uff08Table 6\u53c2\u7167\uff09\u3002
        \n\uff08\u4f8b\uff1a49 Format\u306e\u30a2\u30ec\u30a4\u3067\u3042\u308c\u3070\u300115 \u03bcg\u306eLabeled cRNA\u309232 mL\u306b\u8abf\u6574\u3057\u30018 \u03bcL\u306e3\u2019 Fragmentation Buffer\u3092\u52a0\u3048\u307e\u3059\uff09
        \n\"\"
        \n2. Fragmentation program\uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306694\u2103\u306735\u5206\u306e\u53cd\u5fdc\u3092\u884c\u3044\u3001\u53cd\u5fdc\u7d42\u4e86\u5f8c\u306f\u30b5\u30f3\u30d7\u30eb\u3092\u6c37\u4e0a\u306b\u7f6e\u304d\u307e\u3059\u3002
        \n3. Hybridization\u306b\u9032\u3080\u304b\u3001\u901f\u3084\u304b\u306b\u51b7\u51cd\u4fdd\u5b58\u3057\u307e\u3059\u3002<\/p>\n

        TIP<\/strong>\uff1a<\/strong>STOPPING POINT\uff5eFragmented and labeled ss-cDNA\u30b5\u30f3\u30d7\u30eb\u306f-20\u2103\u3067\u4e00\u6669\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002\u9577\u671f\u4fdd\u5b58\u3059\u308b\u5834\u5408\u306f\u3001-80\u2103\u3067\u4fdd\u5b58\u3059\u308b\u3053\u3068\u3092\u63a8\u5968\u3057\u307e\u3059\u3002<\/p>\n

        Section 8\uff1a\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3<\/h2>\n

        NOTE<\/strong>\uff1a
        \n<\/strong>\u30fb\u8a66\u85ac\u304c\u4f7f\u7528\u524d\u306b\u306f\u5b8c\u5168\u306b\u878d\u89e3\u3057\u3066\u3044\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002DMSO\u306f2\uff5e8\u2103\u3067\u4fdd\u5b58\u3059\u308b\u3068\u56fa\u307e\u308b\u306e\u3067\u3001\u4f7f\u7528\u5f8c\u306f\u5ba4\u6e29\u3067\u4fdd\u5b58\u3057\u305f\u65b9\u304c\u4f7f\u3044\u3084\u3059\u3044\u3067\u3059\u3002
        \n\u30fb\u3053\u3053\u306e\u5de5\u7a0b\u3067\u306f\u5225\u9014\u8cfc\u5165\u3057\u3066\u3044\u305f\u3060\u3044\u305fGeneChip Hybridization, Wash and Stain Kit\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n

        1. 20\u00d7Hybridization Controls\u3092Hybridization Control program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u306665\u2103\u30675\u5206\u52a0\u71b1\u3057\u307e\u3059\u3002
        \nTIP<\/strong>\uff1a<\/strong>\u30ad\u30c3\u30c8\u3054\u8cfc\u5165\u5f8c\u3001\u6700\u521d\u306b65\u2103\u30675\u5206\u52a0\u71b1\u3057\u305f\u5f8c\u306f1.5 mL\u30c1\u30e5\u30fc\u30d6\u306b150 \u03bcL\u305a\u3064\u5c0f\u5206\u3051\u306b\u3057\u3066\u4fdd\u5b58\u3057\u3066\u304a\u304f\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002\u51cd\u7d50\u878d\u89e3\u30923\u56de\u4ee5\u5185\u306b\u6291\u3048\u308b\u305f\u3081\u3067\u3059\u3002
        \n2. \u5ba4\u6e29\u3067Hybridization Master Mix\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002\u4f7f\u7528\u3059\u308b\u30a2\u30ec\u30a4\u306b\u3088\u3063\u3066\u91cf\u304c\u7570\u306a\u308b\u305f\u3081\u3001Table 7\u306b\u5f93\u3063\u3066\u8abf\u88fd\u3057\u3066\u304f\u3060\u3055\u3044\u3002
        \n\"\"
        \n3. Array\u306e\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306b\u5bfe\u5fdc\u3057\u305f\u91cf\u306ePre-Hybridization Mix\u3092 Array\u306b\u6ce8\u5165\u3057\uff08Table 8\u53c2\u7167\uff09\u3001Hybridization Oven\u306745\u2103\u300160 rpm\u306710\uff5e30\u5206\u306ePre-Hybridization\u3092\u884c\u3044\u307e\u3059\u3002
        \n\"\"
        \n4. \u5ba4\u6e29\u3067\u3001\u4f7f\u7528\u3059\u308bArray\u306e\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306b\u5bfe\u5fdc\u3057\u305f\u91cf\u306e\u30d3\u30aa\u30c1\u30f3\u6a19\u8b58\u3055\u308c\u305fcDNA\u30b5\u30f3\u30d7\u30eb\u304c\u5165\u3063\u305f\u30c1\u30e5\u30fc\u30d6\u306bHybridization Master Mix\u3092\u6dfb\u52a0\u3057\u3066\u3001Hybridization Cocktail\u3092\u8abf\u88fd\u3057\u307e\u3059\u3002
        \n\"\"TIP<\/strong>\uff1a<\/strong>STOPPING POINT\uff5eHybridization Cocktail\u306f-20\u2103\u30671\u30ab\u6708\u7a0b\u5ea6\u3001-80\u2103\u30671\u5e74\u7a0b\u5ea6\u4fdd\u5b58\u3067\u304d\u307e\u3059\u3002
        \n5.\u7a4f\u3084\u304b\u306b\u30dc\u30eb\u30c6\u30c3\u30af\u30b9\u306b\u304b\u3051\u3066\u3001\u3088\u304f\u6df7\u5408\u3057\u307e\u3059\u3002
        \n6.Hybridization Cocktail\u3092Hybridization Cocktail program \uff08Table B\u53c2\u7167\uff09\u306b\u5f93\u3063\u3066\u3001\u30b5\u30fc\u30de\u30eb\u30b5\u30a4\u30af\u30e9\u30fc\u306b\u3066\u300199\u2103\uff08\u30c1\u30e5\u30fc\u30d6\u306e\u5834\u5408\uff09\u307e\u305f\u306f95\u2103\uff08\u30d7\u30ec\u30fc\u30c8\u306e\u5834\u5408\uff09\u30675\u5206\u300145\u2103\u30675\u5206\u52a0\u71b1\u3057\u307e\u3059\u3002
        \n7. \u52a0\u71b1\u5f8c\u3001\u77ed\u6642\u9593\u306e\u9060\u5fc3\u3092\u884c\u3044\u307e\u3059\u3002
        \n8. \u5404\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306b\u5bfe\u5fdc\u3057\u305f\u91cf\u3092Array\u306b\u6ce8\u5165\u3057\u307e\u3059\u3002\u6ce8\u5165\u5f8c\u306f\u6ce8\u5165\u53e3\u306eSepta\u306b\u30bf\u30d5\u30b9\u30dd\u30c3\u30c8\u3092\u8cbc\u308a\u307e\u3059\u3002
        \n\"\"TIP<\/strong>\uff1a<\/strong>169 or 400-Format\u306e\u5834\u5408\u3001\u82f1\u6587\u30d7\u30ed\u30c8\u30b3\u30eb\u3067\u306f\u6ce8\u5165\u91cf\u304c80 \u03bcL\u3068\u306a\u3063\u3066\u304a\u308a\u307e\u3059\u304c\u300190 \u03bcL\u306e\u6ce8\u5165\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002\u3053\u308c\u306f\u3001\u30cf\u30a4\u30d6\u30ea\u30c0\u30a4\u30bc\u30fc\u30b7\u30e7\u30f3\u4e2d\u306e\u84b8\u767a\u306b\u3088\u308b\u6790\u51fa\u3092\u9632\u304e\u3084\u3059\u304f\u3059\u308b\u305f\u3081\u3067\u3059\u3002
        \n9. Hybridization Oven\u306745\u2103\u300160 rpm\u306716\u6642\u9593\u306eHybridization\u3092\u884c\u3044\u307e\u3059\u3002<\/p>\n

        Section 9\uff1a\u6d17\u6d44\u3068\u67d3\u8272\u3001\u30b9\u30ad\u30e3\u30f3<\/h2>\n

        \u30fb\u30b5\u30f3\u30d7\u30eb\u60c5\u5831\u767b\u9332\u65b9\u6cd5\u3084Applide Biosystems\u2122 GeneChip Fluidics Station\u3001Scanner\u306e\u64cd\u4f5c\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306f\u3001GeneChip\u2122<\/span>\u00a0Command Console \uff08GCC\u307e\u305f\u306fAGCC\uff09 \u30af\u30a4\u30c3\u30af\u30b9\u30bf\u30fc\u30c8\u30ac\u30a4\u30c9\u3067\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002
        \n\u30fb\u3053\u3053\u3067\u306f\u6d17\u6d44\u3068\u67d3\u8272\u4f5c\u696d\u3067\u4f7f\u7528\u3059\u308b\u8a66\u85ac\u985e\u306e\u5206\u6ce8\u4f5c\u696d\u306a\u3069\u3092\u8a18\u8f09\u3057\u307e\u3059\u3002<\/p>\n

        1.\u00a0 Fluidics Station\u306e\u96fb\u6e90\u3092\u5165\u308c\u3001Wash A\u3001Wash B\u3001\u8d85\u7d14\u6c34\u306e\u5165\u3063\u305f\u30dc\u30c8\u30eb\u3092\u30bb\u30c3\u30c8\u3057\u3066\u3001GCC Fluidics Control\u203b<\/sup>\u3092\u8d77\u52d5\u3057\u3001Prime\u3092\u958b\u59cb\u3057\u307e\u3059\u3002
        \n\u203bAGCC Fluidics Control\u3068\u3082\u547c\u3073\u307e\u3059\u3002
        \n2. Hybridization\u3092\u884c\u3063\u3066\u3044\u308bArray\u306e\u679a\u6570\u306b\u5408\u308f\u305b\u3066\u3001Stain Cocktail 1\u3001Stain Cocktail 2\u3001Array Holding Buffer\u3092\u305d\u308c\u305e\u308c\u5206\u6ce8\u3057\u305fvial\u3092\u6e96\u5099\u3057\u307e\u3059\u3002
        \n\u30fbStain Cocktail 1\u3000600 \u03bcL \uff08\u906e\u5149\u30c1\u30e5\u30fc\u30d6\uff09
        \n\u30fbStain Cocktail 2\u3000600 \u03bcL \uff08\u900f\u660e\u30c1\u30e5\u30fc\u30d6\uff09
        \n\u30fbArray Holding Buffer 800 \u03bcL \uff08\u900f\u660e\u30c1\u30e5\u30fc\u30d6\uff09
        \nTIP<\/strong>\uff1a<\/strong>Stain Cocktail 1\u7528\u306b\u906e\u5149\u30c1\u30e5\u30fc\u30d6\u306e\u3054\u7528\u610f\u304c\u96e3\u3057\u3044\u5834\u5408\u306f\u3001\u900f\u660e\u30c1\u30e5\u30fc\u30d6\u306b\u5165\u308c\u3001\u4f7f\u7528\u76f4\u524d\u307e\u3067\u906e\u5149\u306e\u74b0\u5883\u4e0b\u306b\u7f6e\u3044\u3066\u304a\u3044\u3066\u304f\u3060\u3055\u3044\u3002
        \n3. Hybridization\u304c\u7d42\u4e86\u3057\u305fArray\u3092Hybridization Oven\u304b\u3089\u53d6\u308a\u51fa\u3057\u3066\u30bf\u30d5\u30b9\u30dd\u30c3\u30c8\u3092\u306f\u304c\u3057\u3001Hybridization Cocktail\u3092\u56de\u53ce\u3057\u307e\u3059\uff08\u30c7\u30fc\u30bf\u304c\u78ba\u5b9f\u306b\u53d6\u308c\u305f\u3053\u3068\u3092\u78ba\u8a8d\u3067\u304d\u308b\u307e\u3067\u306f-20\u2103\u3067\u4fdd\u5b58\uff09\u3002
        \n4. GCC Fluidics Control\u3067Wash and Stain\u3092\u958b\u59cb\u3057\u307e\u3059\u3002
        \nArray\u306eFormat\u306b\u3088\u3063\u3066\u4f7f\u7528\u3059\u308b\u30d7\u30ed\u30c8\u30b3\u30eb\u304c\u7570\u306a\u308a\u307e\u3059\u3002
        \n\"\"
        \n\u307e\u305f\u3001\u3053\u306e\u30bf\u30a4\u30df\u30f3\u30b0\u3067Scanner\u306e\u96fb\u6e90\u3092\u5165\u308c\u3066\u304a\u304d\u307e\u3059\u3002
        \n5. Fluidics Station\u306e\u6db2\u6676\u753b\u9762\u306bEJECT & INSPECT CARTRIDGE\u304c\u8868\u793a\u3055\u308c\u305f\u3089Array\u3092\u53d6\u308a\u51fa\u3057\u3001\u30ac\u30e9\u30b9\u9762\u306b\u6c17\u6ce1\u304c\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3057\u3001Septa\u306b\u30bf\u30d5\u30b9\u30dd\u30c3\u30c8\u3092\u8cbc\u308a\u307e\u3059\u3002\u3082\u3057\u3001\u6c17\u6ce1\u304c\u8a8d\u3081\u3089\u308c\u305f\u3089\u3001\u30de\u30a4\u30af\u30ed\u30d4\u30da\u30c3\u30c8\u3092\u4f7f\u3063\u3066Array Holding Buffer\u3092\u6c17\u6ce1\u304c\u5165\u3089\u306a\u3044\u3088\u3046\u306b\u5165\u308c\u76f4\u3057\u3066\u304b\u3089\u30bf\u30d5\u30b9\u30dd\u30c3\u30c8\u3092\u8cbc\u308a\u307e\u3059\u3002
        \n6.GCC Scan Control\u203b<\/sup>\u3092\u8d77\u52d5\u3057\u3001Array\u306eScan\u3092\u958b\u59cb\u3057\u307e\u3059\u3002
        \n\u203bAGCC Scan Control\u3068\u3082\u547c\u3073\u307e\u3059\u3002
        \n7. Fluidics Station\u3092\u7d9a\u3051\u3066\u4f7f\u7528\u3059\u308b\u4e88\u5b9a\u304c\u306a\u3051\u308c\u3070\u3001Wash A\u3001Wash B\u306e\u30dc\u30c8\u30eb\u3092\u8d85\u7d14\u6c34\u306e\u5165\u3063\u305f\u30dc\u30c8\u30eb\u306b\u4ea4\u63db\u3057\u3001GCC Fluidics Control\u3067Shut Down\u3092\u884c\u3044\u307e\u3059\u3002Shut Down\u7d42\u4e86\u5f8c\u3001GCC Fluidics Control\u3092\u9589\u3058\u3001Fluidics Station\u306e\u96fb\u6e90\u3092\u5207\u308a\u307e\u3059\u3002
        \n8. Array\u306eScan\u304c\u7d42\u4e86\u3057\u305f\u3089GCC Scan Control\u3092\u9589\u3058\u3001Scanner\u306e\u96fb\u6e90\u3092\u5207\u308a\u307e\u3059\u3002<\/p>\n

        \u307e\u3068\u3081<\/h2>\n

        \u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u95a2\u3059\u308b\u3054\u4e0d\u660e\u70b9\u3084\u6c17\u306b\u306a\u308b\u70b9\u306f\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8<\/a>\u307e\u3067\u304a\u554f\u3044\u5408\u308f\u305b\u304f\u3060\u3055\u3044\u3002<\/p>\n

        \u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

        \u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3092\u4f7f\u7528\u3057\u305f\u907a\u4f1d\u5b50\u767a\u73fe\u89e3\u6790\u306f\u73fe\u5728\u3082\u3063\u3068\u3082\u30dd\u30d4\u30e5\u30e9\u30fc\u306a\u65b9\u6cd5\u3067\u3001\u767a\u73fe\u6bd4\u8f03\u7814\u7a76\u3084\u30d0\u30a4\u30aa\u30de\u30fc\u30ab\u30fc\u63a2\u7d22\u306b\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u5b9f\u7e3e\u304c\u3042\u308a\u307e\u3059\u3002\u8fd1\u5e74\u3082\u3001\u9078\u629e\u7684\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u3084IncRNA\u89e3\u6790\u3092\u4e2d\u5fc3\u306b\u30a2\u30ec\u30a4\u306f\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u304a\u308a<\/p>\n","protected":false},"author":975,"featured_media":9832,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[71],"division":[],"class_list":{"0":"post-10024","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-microarray","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"\n3\u2019IVT Plus Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb - Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/3ivtplusreagentkit_protocol_ma_gsd_mkt_1\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"3\u2019IVT Plus Reagent Kit\u3092\u4f7f\u7528\u3057\u305f\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u7528\u30b5\u30f3\u30d7\u30eb\u8abf\u88fd\u65e5\u672c\u8a9e\u7248\u7c21\u6613\u30d7\u30ed\u30c8\u30b3\u30eb\" \/>\n<meta property=\"og:description\" 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