{"id":10050,"date":"2021-09-22T14:25:29","date_gmt":"2021-09-22T05:25:29","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=10050"},"modified":"2025-04-01T16:22:13","modified_gmt":"2025-04-01T07:22:13","slug":"antibody_characterization_pca_bid_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/antibody_characterization_pca_bid_ts_1\/","title":{"rendered":"\u6297\u4f53\u6cd5\u306b\u3088\u308b\u5e79\u7d30\u80de\u30ad\u30e3\u30e9\u30af\u30bf\u30ea\u30bc\u30fc\u30b7\u30e7\u30f3\u8b1b\u5ea71\u3000\u591a\u80fd\u6027\u5e79\u7d30\u80de"},"content":{"rendered":"

\u591a\u80fd\u6027\u5e79\u7d30\u80de\u3068\u306f\uff1f<\/h2>\n

\u30d2\u30c8\u591a\u80fd\u6027\u5e79\u7d30\u80de\uff08PSC\uff09\u306f\u3001\u81ea\u5df1\u3092\u969b\u9650\u306a\u304f\u518d\u751f\u3057\u3001\u9069\u5207\u306a\u5fae\u5c0f\u74b0\u5883\u306b\u304a\u3044\u3066\u30d2\u30c8\u4f53\u5185\u306b\u5b58\u5728\u3059\u308b\u307b\u307c\u5168\u3066\u306e\u7d30\u80de\u30bf\u30a4\u30d7\u306b\u5206\u5316\u3059\u308b\u80fd\u529b\u3092\u7279\u5fb4\u3068\u3057\u307e\u3059\u3002PSC\u306f\u3001\u80da\u6027\u5e79\u7d30\u80de\uff08ESC\uff09\u3068\u4eba\u5de5\u591a\u80fd\u6027\u5e79\u7d30\u80de\uff08iPSC\uff09\u306e2\u7a2e\u985e\u304c\u4e00\u822c\u7684\u306b\u7814\u7a76\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u56f32.1\uff09\u3002\u30d2\u30c8ESC\u306f\u3001\u767a\u751f\u4e2d\u306e\u80da\u306e\u80da\u76e4\u80de\u671f\u306e\u5185\u90e8\u7d30\u80de\u584a\u304b\u3089\u5358\u96e2\u3055\u308c\u307e\u3059*1<\/sup> \u3002iPSC\u306f\u3001ESC\u3068\u540c\u7b49\u307e\u305f\u306f\u985e\u4f3c\u3059\u308b\u7d30\u80de\u3067\u3059\u304c\u3001\u6210\u4f53\u4f53\u7d30\u80de\u3092\u30ea\u30d7\u30ed\u30b0\u30e9\u30df\u30f3\u30b0\u3059\u308b\u305f\u3081\u306eOCT3\/4\u3001KLF4\u3001SOX2\u3001\u304a\u3088\u3073c-MYC\u306a\u3069\u306e\u30ea\u30d7\u30ed\u30b0\u30e9\u30df\u30f3\u30b0\u56e0\u5b50\u306e\u767a\u73fe\u3092\u7d4c\u3066\u751f\u6210\u3057\u307e\u3059*2,3<\/sup> \u3002iPSC\u306f\u3001\u60a3\u8005\u306e\u5e79\u7d30\u80de\u306e\u8a98\u5c0e\u3092\u7c21\u7d20\u5316\u3059\u308b\u3053\u3068\u306b\u3088\u308a\u5e79\u7d30\u80de\u7814\u7a76\u306b\u9769\u547d\u3092\u3082\u305f\u3089\u3057\u307e\u3057\u305f\u3002\u3053\u308c\u306b\u3088\u308a\u3001iPSC\u3092\u4f7f\u7528\u3057\u3066\u3001\u30b7\u30e3\u30fc\u30ec\u4e2d\u3067\u75be\u60a3\u7279\u7570\u7684\u306a\u7d30\u80de\u30bf\u30a4\u30d7\u306e\u30e2\u30c7\u30eb<\/a>\u3092\u4f5c\u88fd\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u3089\u306e\u30e2\u30c7\u30eb\u306f\u3001\u75be\u60a3\u75c5\u614b\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u5b9a\u7fa9\u3059\u308b\u306e\u306b\u6709\u7528\u3067\u3001\u6cbb\u7642\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u540c\u5b9a\u3084\u5275\u85ac\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u679c\u305f\u3059\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u6a5f\u80fd\u7684\u306a\u6210\u719f\u7d30\u80de\u306b\u5206\u5316\u3059\u308b\u3088\u3046\u65b9\u5411\u4ed8\u3051\u308b\u3053\u3068\u304c\u53ef\u80fd\u306aiPSC\u3092\u7121\u5236\u9650\u306b\u4f9b\u7d66\u3067\u304d\u308b\u3053\u3068\u306f\u3001\u7cd6\u5c3f\u75c5\u3001\u809d\u75be\u60a3\u3001\u304a\u3088\u3073\u30d1\u30fc\u30ad\u30f3\u30bd\u30f3\u75c5\u3084\u30a2\u30eb\u30c4\u30cf\u30a4\u30de\u30fc\u75c5\u306a\u3069\u306e\u69d8\u3005\u306a\u75be\u60a3\u306b\u5bfe\u3059\u308b\u7d30\u80de\u7642\u6cd5<\/a>\u306e\u305f\u3081\u306e\u539f\u6750\u6599\u3068\u3057\u3066\u975e\u5e38\u306b\u6709\u671b\u8996\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\n\"\"<\/p>\n

\u591a\u80fd\u6027\u5e79\u7d30\u80de\u306e\u30ad\u30e3\u30e9\u30af\u30bf\u30ea\u30bc\u30fc\u30b7\u30e7\u30f3<\/h2>\n

iPSC\u304a\u3088\u3073ESC\u3067\u306f\u3001\u672a\u5206\u5316\u5e79\u7d30\u80de\u72b6\u614b\u3092\u7dad\u6301\u3059\u308b\u305f\u3081\u306bNanog\u304a\u3088\u3073OCT4\u306a\u3069\u306e\u30ad\u30fc\u8ee2\u5199\u56e0\u5b50\u306e\u767a\u73fe\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059*4<\/sup> \u3002\u3053\u308c\u3089\u306e\u8ee2\u5199\u56e0\u5b50\u306f\u3001\u7d30\u80de\u306e\u5206\u88c2\u3001\u5206\u5316\u3001\u304a\u3088\u3073\u767a\u9054\u306b\u91cd\u8981\u306a\u907a\u4f1d\u5b50\u3092\u8abf\u7bc0\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u8ee2\u5199\u56e0\u5b50\u306e\u4ed6\u306b\u3001TRA-1-60\u3001TRA-1-81\u3001SSEA3\u3001\u304a\u3088\u3073SSEA4\u306a\u3069\u306e\u7279\u5b9a\u306e\u4e00\u9023\u306e\u7d30\u80de\u8868\u9762\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u901a\u5e38\u3001PSC\u306b\u3088\u3063\u3066\u767a\u73fe\u3057\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u30de\u30fc\u30ab\u30fc\u306e\u5b58\u5728\u306e\u691c\u51fa\u306f\u3001\u65b0\u305f\u306b\u8a98\u5c0e\u3055\u308c\u305fiPSC\u682a\uff08iPSC line\uff09\u304a\u3088\u3073ESC\u682a\uff08ES Cline\uff09\u306e\u30ad\u30e3\u30e9\u30af\u30bf\u30ea\u30bc\u30fc\u30b7\u30e7\u30f3\u306b\u304a\u3051\u308b\u6700\u521d\u306e\u30b9\u30c6\u30c3\u30d7\u306e1\u3064\u3067\u3059\u3002\u7279\u5b9a\u306e\u7d30\u80de\u306e\u67d3\u8272\u306f\u3001\u3053\u308c\u3089\u306e\u78ba\u7acb\u3055\u308c\u305f\u591a\u80fd\u6027\u30de\u30fc\u30ab\u30fc\u306b\u5bfe\u3059\u308b\u6297\u4f53\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u9054\u6210\u3067\u304d\u307e\u3059\uff08\u88682.1\uff09\u3002\"\"<\/p>\n

\u751f\u7d30\u80de\u306e\u514d\u75ab\u67d3\u8272<\/h3>\n

\u7d30\u80de\u8868\u9762\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u5168\u7d30\u80de\u306b\u8272\u7d20\u6a19\u8b58\u6297\u4f53\u3067\u8fc5\u901f\u306b\u67d3\u8272\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u653e\u5c04\u7dda\u7167\u5c04\u3057\u305f\u30de\u30a6\u30b9\u80ce\u4ed4\u7dda\u7dad\u82bd\u7d30\u80de\uff08MEF\uff09\u306e\u30d5\u30a3\u30fc\u30c0\u30fc\u5c64\u4e0a\u3067\u57f9\u990a\u3057\u305fiPSC\u30b3\u30ed\u30cb\u30fc\u4e0a\u306ePSC\u8868\u9762\u30de\u30fc\u30ab\u30fcTRA-1-60\u306e\u751f\u7d30\u80de\u514d\u75ab\u67d3\u8272\u306e\u4f8b\u3092\u56f32.2\u306b\u793a\u3057\u307e\u3059\u3002CD44\u306f\u3001MEF\u3092\u542b\u3080\u591a\u304f\u306e\u5206\u5316\u7d30\u80de\u3067\u767a\u73fe\u3055\u308c\u308b\u8868\u9762\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u304c\u3001PSC\u3067\u306f\u767a\u73fe\u3057\u307e\u305b\u3093\u3002\u65b0\u305f\u306b\u8a98\u5c0e\u3057\u305fiPSC\u30b3\u30ed\u30cb\u30fc\u304c\u5b8c\u5168\u306b\u30ea\u30d7\u30ed\u30b0\u30e9\u30df\u30f3\u30b0\u3055\u308c\u3066\u3044\u308b\u304b\u306e\u78ba\u8a8d\u306b\u306f\u3001TRA-1-60\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u67d3\u8272\u3068CD44\u306e\u30cd\u30ac\u30c6\u30a3\u30d6\u67d3\u8272\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002
\n\"\"<\/p>\n

\u56fa\u5b9a\u7d30\u80de\u306e\u514d\u75ab\u67d3\u8272<\/h3>\n

iPSC\u30af\u30ed\u30fc\u30f3\u306e\u54c1\u8cea\u306e\u4fe1\u983c\u6027\u3092\u5411\u4e0a\u3055\u305b\u308b\u305f\u3081\u306b\u306f\u3001\u308f\u305a\u304b1\u3064\u304b2\u3064\u3067\u306a\u304f\u3001\u8907\u6570\u306ePSC\u30de\u30fc\u30ab\u30fc\u306e\u767a\u73fe\u306b\u3064\u3044\u3066\u78ba\u8a8d\u3059\u308b\u3053\u3068\u304c\u63a8\u5968\u3055\u308c\u307e\u3059\u3002Oct4\u3001Nanog\u3001Lin28\u3001\u304a\u3088\u3073Sox2\u306a\u3069\u306e\u4e00\u90e8\u306e\u30de\u30fc\u30ab\u30fc\u306f\u7d30\u80de\u5185\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308b\u305f\u3081\u3001\u3053\u308c\u3089\u306e\u30de\u30fc\u30ab\u30fc\u306e\u67d3\u8272\u3092\u884c\u3046\u969b\u306f\u56fa\u5b9a\u304a\u3088\u3073\u900f\u904e\u51e6\u7406\u304c\u5fc5\u8981\u3068\u3055\u308c\u307e\u3059\uff08\u56f32.3\uff09\u3002\u3053\u308c\u3089\u306e\u30de\u30fc\u30ab\u30fc\u304a\u3088\u3073\u4ed6\u306ePSC\u30de\u30fc\u30ab\u30fc\u306b\u5bfe\u3059\u308b\u8272\u7d20\u6a19\u8b58\u4e00\u6b21\u6297\u4f53\u3092\u4f7f\u7528\u3059\u308b\u3068\uff08\u88682.1\uff09\u3001\u4e8c\u6b21\u6297\u4f53\u67d3\u8272\u5de5\u7a0b\u3092\u56de\u907f\u3067\u304d\u3001\u5b9f\u9a13\u306b\u304b\u304b\u308b\u6642\u9593\u3068\u30b3\u30b9\u30c8\u3092\u524a\u6e1b\u3057\u3001\u591a\u91cd\u67d3\u8272\u3092\u884c\u3046\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\uff08\u56f32.4\uff09\u3002
\n\"\"<\/p>\n

\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30c8\u30ea\u30fc<\/h3>\n

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\n\"\"
\nPSC\u306e\u89e3\u6790\u304a\u3088\u3073\u81ea\u5df1\u8907\u88fd\u907a\u4f1d\u5b50\u7523\u7269\u306e\u5b58\u5728\u3084\u4f53\u7d30\u80de\u907a\u4f1d\u5b50\u7523\u7269\u306e\u4e0d\u5728\u306b\u3064\u3044\u3066\u78ba\u8a8d\u3059\u308b\u3053\u3068\u306f\u91cd\u8981\u3067\u3059\u304c\u3001PSC\u682a\u306e\u6a5f\u80fd\u7684\u591a\u80fd\u6027\u306b\u3064\u3044\u3066\u306e\u78ba\u8a8d\u306b\u306f\u5341\u5206\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3053\u306e\u4ed6\u306e\u91cd\u8981\u306a\u8a66\u9a13\u306b\u3001PSC\u306e\u4e09\u80da\u8449\uff08\u5916\u80da\u8449\u3001\u4e2d\u80da\u8449\u3001\u5185\u80da\u8449\uff09\u306e\u5404\u7d30\u80de\u3078\u306e\u5206\u5316\u80fd\u306e\u8a55\u4fa1\u304c\u3042\u308a\u307e\u3059\uff08\u56f32.6\uff09\u3002\u3053\u306e\u8a66\u9a13\u306f\u3001 in vitro \u304a\u3088\u3073 in vitro\u306e\u3044\u305a\u308c\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u306b\u3088\u3063\u3066\u3082\u9054\u6210\u3067\u304d\u307e\u3059\u3002
\n\"\"<\/p>\n

\u30c6\u30e9\u30c8\u30fc\u30de\u5f62\u6210<\/h3>\n

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\u80da\u69d8\u4f53\uff08EB\uff09\u5f62\u6210<\/h3>\n

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\n\"\"<\/p>\n

\u53c2\u8003\u6587\u732e\uff1a<\/strong>
\n1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. (1998) Embryonic stem cell lines derived from human blastocysts. Science 282(5391):1145\u20131147.
\n2. Takahashi K, Yamanaka S (2006) Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126(4):663\u2013676.
\n3. Yu J, Vodyanik MA, Smuga-Otto K et al. (2007) Induced pluripotent stem cell lines derived from human somatic cells. Science 318(5858):1917\u20131920.
\n4. Pan GJ, Thomson JA (2007) Nanog and transcriptional networks in embryonic stem cell pluripotency. Cell Res 17(1):42\u201349.<\/p>\n

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\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

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