{"id":10084,"date":"2021-09-29T16:18:45","date_gmt":"2021-09-29T07:18:45","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=10084"},"modified":"2025-04-01T16:22:50","modified_gmt":"2025-04-01T07:22:50","slug":"antibody_characterization_pca_bid_ts_3","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/antibody_characterization_pca_bid_ts_3\/","title":{"rendered":"\u6297\u4f53\u6cd5\u306b\u3088\u308b\u5e79\u7d30\u80de\u30ad\u30e3\u30e9\u30af\u30bf\u30ea\u30bc\u30fc\u30b7\u30e7\u30f3\u8b1b\u5ea73\u3000\u591a\u80fd\u6027\u5e79\u7d30\u80de\u306e\u5206\u5316"},"content":{"rendered":"

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\n\u54fa\u4e73\u985e\u306e\u30cb\u30e5\u30fc\u30ed\u30f3\u65b0\u751f\u306f\u3001\u795e\u7d4c\u5916\u80da\u8449\u306e\u8a98\u5c0e\u306b\u59cb\u307e\u308a\u3001\u795e\u7d4c\u677f\u304c\u5f62\u6210\u3055\u308c\u305f\u5f8c\u3001\u305d\u308c\u304c\u9686\u8d77\u3057\u3066\u30d2\u30c0\u3068\u306a\u308a\u795e\u7d4c\u7ba1\u304c\u5f62\u6210\u3055\u308c\u307e\u3059\u3002\u3053\u308c\u3089\u306e\u69cb\u9020\u306f\u3001\u795e\u7d4c\u4e0a\u76ae\u524d\u99c6\u7d30\u80de\uff08NEP\uff09\u306e\u5c64\u304b\u3089\u5f62\u6210\u3055\u308c\u307e\u3059\u3002NEP\u306f\u8fc5\u901f\u306b\u539f\u59cb\u795e\u7d4c\u5e79\u7d30\u80de\uff08NSC\uff09\u306b\u5909\u63db\u3055\u308c\u307e\u3059*2<\/sup>\u3002NSC\u306f\u81ea\u5df1\u8907\u88fd\u80fd\u3092\u6301\u3064\u591a\u5206\u5316\u6027\u524d\u99c6\u7d30\u80de\u3067\u3001\u6210\u4f53\u306a\u3069\u306e\u4e2d\u67a2\u795e\u7d4c\u7cfb\u306b\u5b58\u5728\u3057\u307e\u3059\u3002NSC\u304a\u3088\u3073\u795e\u7d4c\u524d\u99c6\u7d30\u80de\u306f\u3001\u767a\u751f\u4e2d\u306b\u308f\u305f\u3063\u3066\u5b58\u5728\u3057\u3001\u6210\u4f53\u795e\u7d4c\u7cfb\u306b\u3082\u5b58\u5728\u3057\u7d9a\u3051\u307e\u3059\u3002\u8907\u6570\u306e\u30af\u30e9\u30b9\u306eNSC\u306f\u3001\u4e92\u3044\u306b\u5206\u5316\u80fd\u529b\u3001\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u53cd\u5fdc\u6027\u3001\u304a\u3088\u3073\u8868\u9762\u6297\u539f\u7279\u6027\u304c\u7570\u306a\u308b\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u3066\u3044\u307e\u3059\u3002in vitro \u3067\u3001\u591a\u80fd\u6027\u5e79\u7d30\u80de\uff08PSC\uff09\u304b\u3089\u8a98\u5c0e\u3055\u308c\u305fNSC\u306f\u3001\u8ee2\u5199\u56e0\u5b50SOX1\u3001SOX2\u3001PAX6\u3001\u304a\u3088\u3073VI\u578b\u4e2d\u9593\u7cfb\u30d5\u30a3\u30e9\u30e1\u30f3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u30cd\u30b9\u30c1\u30f3\u306a\u3069\u306e\u7279\u5b9a\u306e\u4e00\u9023\u306e\u30de\u30fc\u30ab\u30fc\u3092\u767a\u73fe\u3059\u308b\u3053\u3068\u3067\u7279\u5fb4\u4ed8\u3051\u3089\u308c\u307e\u3059\uff08\u56f35.1\uff09\u3002SOX1\u306f\u3001\u521d\u671f\u306e\u30cb\u30e5\u30fc\u30ed\u30f3\u65b0\u751f\u306e\u5f15\u304d\u91d1\u3068\u306a\u308a\u3001NSC\u3092\u672a\u5206\u5316\u72b6\u614b\u306b\u7dad\u6301\u3059\u308b\u6a5f\u80fd\u304c\u3042\u308a\u307e\u3059\u3002SOX2\u306f\u3001\u30ad\u30fc\u8ee2\u5199\u56e0\u5b50\u3067\u3001PSC\u304a\u3088\u3073NSC\u306e\u81ea\u5df1\u8907\u88fd\u7279\u6027\u3092\u7dad\u6301\u3057\u307e\u3059\u3002PAX6\u306f\u3001\u30cb\u30e5\u30fc\u30ed\u30f3\u65b0\u751f\u521d\u671f\u306b\u795e\u7d4c\u5916\u80da\u8449\u30de\u30fc\u30ab\u30fc\u3068\u3057\u3066\u6a5f\u80fd\u3057\u307e\u3059\u3002NSC\u306f\u4e00\u5ea6\u6a39\u7acb\u3055\u308c\u308b\u3068\u3001PAX6\u3092\u767a\u73fe\u3057\u7d9a\u3051\u3066\u3082\u3001\u305d\u306e\u7d30\u80de\u904b\u547d\u306f\u7279\u5b9a\u306e\u795e\u7d4c\u30b5\u30d6\u30bf\u30a4\u30d7\u306b\u9650\u5b9a\u3055\u308c\u307e\u3059*3<\/sup>\u3002\u30cd\u30b9\u30c1\u30f3\u3082\u3001CNS\u306e\u767a\u9054\u521d\u671f\u306e\u9593\u306b\u767a\u73fe\u3057\u307e\u3059\u304c\u3001NSC\u304c\u5206\u5316\u3057\u3001\u6210\u719f\u30cb\u30e5\u30fc\u30ed\u30f3\u3084\u30b0\u30ea\u30a2\u7d30\u80de\u306b\u306a\u308b\u3068\u6d88\u5931\u3057\u307e\u3059\u3002
\n\u30cb\u30e5\u30fc\u30ed\u30f3\u306e\u5206\u5316\u4e2d\u3001NSC\u306f\u9032\u884c\u6027\u306e\u7d30\u80de\u7cfb\u8b5c\u306e\u5236\u9650\u3092\u53d7\u3051\uff08\u56f35.2\uff09\u3001\u30b0\u30ea\u30a2\u524d\u99c6\u7d30\u80de\uff08CD44+\/A2B5+\uff09\u304c\u5f62\u6210\u3055\u308c\u3001\u30a2\u30b9\u30c8\u30ed\u30b5\u30a4\u30c8\uff08GFAP+\uff09\uff08\u56f35.3\uff09\u304a\u3088\u3073\u30aa\u30ea\u30b4\u30c7\u30f3\u30c9\u30ed\u30b5\u30a4\u30c8\uff08Galc+\u304a\u3088\u3073O4+\uff09\u3068\u306a\u308a\u307e\u3059\u3002\u7d30\u80de\u7cfb\u8b5c\u306e\u5236\u9650\u306b\u304a\u3051\u308b\u4ed6\u306e\u5206\u5c90\u306b\u306f\u3001\u30c9\u30fc\u30d1\u30df\u30f3\u4f5c\u52d5\u6027\uff08DA\uff09\u30cb\u30e5\u30fc\u30ed\u30f3\uff08\u56f35.5\uff09\u3001GABA\u4f5c\u52d5\u6027\uff08GABA\uff09\u30cb\u30e5\u30fc\u30ed\u30f3\uff08\u56f35.6\uff09\u3001\u304a\u3088\u3073\u904b\u52d5\u30cb\u30e5\u30fc\u30ed\u30f3\u306a\u3069\u306e\u69d8\u3005\u306a\u30bf\u30a4\u30d7\u306e\u30cb\u30e5\u30fc\u30ed\u30f3\uff08\u56f35.4\uff09\u306e\u5f62\u6210\u306b\u3064\u306a\u304c\u308b\u795e\u7d4c\u7cfb\u7d71\u306e\u7d4c\u8def\u304c\u3042\u308a\u307e\u3059\u3002NSC\u306e\u5358\u96e2\u6cd5\u3001PSC\u304b\u3089\u306eNSC\u306e\u8a98\u5c0e\u6cd5\u3001\u304a\u3088\u3073 in vitro \u3067\u306e\u5897\u6b96\u6cd5\u306e\u958b\u767a\u306b\u304a\u3051\u308b\u9032\u6b69\u306f\u3001NSC\u3092\u57fa\u790e\u7684\u306a\u767a\u9054\u7814\u7a76\u3084\u7d30\u80de\u7642\u6cd5\u306e\u305f\u3081\u306e\u6709\u671b\u306a\u30c4\u30fc\u30eb\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u3068\u3057\u307e\u3057\u305f*2<\/sup>\u3002
\nPSC\u304b\u3089DA\u30cb\u30e5\u30fc\u30ed\u30f3\u306a\u3069\u306e\u7279\u5b9a\u306e\u795e\u7d4c\u7d30\u80de\u30b5\u30d6\u30bf\u30a4\u30d7\u306b\u76f4\u63a5\u8a98\u5c0e\u3059\u308b\u305f\u3081\u306e\u69d8\u3005\u306a\u5206\u5316\u8a98\u5c0e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3082\u958b\u767a\u3055\u308c\u3066\u304d\u3066\u3044\u307e\u3059*4<\/sup>\u3002 \u3053\u308c\u3089\u306e\u30a2\u30d7\u30ed\u30fc\u30c1\u306f\u3001\u7814\u7a76\u5bfe\u8c61\u306e\u75be\u60a3\u306b\u95a2\u9023\u3059\u308b\u7279\u5b9a\u306e\u30bf\u30a4\u30d7\u306e\u30cb\u30e5\u30fc\u30ed\u30f3\u306e\u751f\u6210\u3092\u53ef\u80fd\u3068\u3057\u307e\u3059\u3002<\/p>\n

\"\"
\n\"\"
\n\"\"
\n\"\"
\n\"\"
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\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u5e79\u7d30\u80de\u7814\u7a76\u7528\u6297\u4f53\u30ac\u30a4\u30c9\u30d6\u30c3\u30af<\/h2>\n

\u672c\u30ac\u30a4\u30c9\u30d6\u30c3\u30af\u3067\u306f\u3001\u5e79\u7d30\u80de\u7814\u7a76\u306b\u95a2\u3059\u308b\u6df1\u3044\u77e5\u8b58\u306e\u7fd2\u5f97\u3001\u304a\u3088\u3073\u6700\u65b0\u306e\u7814\u7a76\u306b\u5f79\u7acb\u3064\u6297\u4f53\u95a2\u9023\u88fd\u54c1\u306e\u60c5\u5831\u3092\u63d0\u4f9b\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

\u53c2\u8003\u6587\u732e:<\/strong>
\n1. Ichida JK, Kiskinis E (2015) Probing disorders of the nervous system using reprogramming approaches. EMBOJ 34(11):1456\u20131477.
\n2. Gage FH, Temple S (2013) Neural stem cells: generating and regenerating the brain. Neuron 80(3):588\u2013601.
\n3. Zhang X, Huang CT, Chen J et al. (2010) Pax6 is a human neuroectoderm cell fate determinant. Cell Stem Cell 7(1):90\u2013100.
\n4. Kriks S, Shim JW, Piao J et al. (2011) Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson\u2019s disease. Nature 480(7378):547\u2013551.<\/p>\n

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