{"id":11048,"date":"2022-02-02T13:56:14","date_gmt":"2022-02-02T04:56:14","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=11048"},"modified":"2025-04-23T14:10:19","modified_gmt":"2025-04-23T05:10:19","slug":"str_analysis_ce_ngs_gsd_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/str_analysis_ce_ngs_gsd_ts_1\/","title":{"rendered":"STR\u89e3\u6790\u3092\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\uff08CE\uff09\u3068\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\uff08NGS\uff09\u3067"},"content":{"rendered":"

\"\"\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u5206\u91ce\u306b\u3082\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\uff08CE\uff09\u306e\u5229\u7528\u3060\u3051\u3067\u306a\u304f\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\uff08NGS\uff09\u3092\u7528\u3044\u305f\u89e3\u6790\u304c\u59cb\u307e\u308a\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u4eca\u56de\u306f\u3001STR\u89e3\u6790\u306e\u6b74\u53f2\u3068\u305d\u308c\u305e\u308c\u306e\u89e3\u6790\u65b9\u6cd5\u306e\u7279\u5fb4\u3064\u3044\u3066\u3001\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01
\n\u30fb\u6cd5\u533b\u5b66\u3067DNA\u9451\u5b9a\u3092\u59cb\u3081\u3089\u308c\u305f\u65b9
\n\u30fbNGS\u3092\u7528\u3044\u305fSTR\u89e3\u6790\u3092\u691c\u8a0e\u4e2d\u306e\u65b9
\n\u30fbCE\u3068NGS\u306e\u9055\u3044\u3092\u77e5\u308a\u305f\u3044\u65b9<\/p>\n

DNA\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u306e\u59cb\u307e\u308a<\/h2>\n

DNA\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u306f\u30011985\u5e74\u4ee5\u6765\u3001\u30df\u30cb\u30b5\u30c6\u30e9\u30a4\u30c8\u3068\u30b5\u30b6\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u304c\u79d1\u5b66\u635c\u67fb\u3067\u4f7f\u7528\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002Alec Jeffreys\u6c0f1,2<\/sup>\u306f\u3001DNA\u7279\u6027\u306b\u57fa\u3065\u3044\u3066\u500b\u4eba\u3092\u8b58\u5225\u3059\u308b\u3068\u3044\u3046\u30a2\u30a4\u30c7\u30a2\u3092\u5c0e\u304d\u51fa\u3057\u3001\u3053\u306e\u624b\u6cd5\u3092\u4f7f\u7528\u3057\u3066\u6700\u521d\u306e\u79fb\u6c11\u554f\u984c\u3092\u89e3\u6c7a\u3057\u307e\u3057\u305f\u3002
\n1990\u5e74\u4ee3\u521d\u982d\u306b\u306f\u77ed\u9396\u30ea\u30d4\u30fc\u30c8\uff08short tandem repeat\uff1aSTR\uff09\u304c\u65b0\u3057\u3044\u591a\u578bDNA\u30de\u30fc\u30ab\u30fc\u30bf\u30a4\u30d73,4<\/sup>\u3068\u3057\u3066\u5c0e\u5165\u3055\u308c\u3001\u5211\u4e8b\u4e8b\u4ef6\u3001\u7236\u89aa\u3068\u8840\u65cf\u95a2\u4fc2\u306e\u5206\u6790\u3001\u304a\u3088\u3073\u884c\u65b9\u4e0d\u660e\u8005\u306e\u8b58\u52255,6<\/sup>\u306b\u304a\u3051\u308b\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u30b4\u30fc\u30eb\u30c9\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306b\u306a\u308a\u307e\u3057\u305f\u3002<\/p>\n

STR\u89e3\u6790\u306e\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u5316<\/h2>\n

\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\uff08CE\uff09<\/a>\u3092\u7528\u3044\u305fSTR\u89e3\u6790\u3067\u306f\u3001Applied Biosystems\u2122 AmpFLSTR\u2122 Blue\u3001Green\u3001Yellow PCR Amplification Kit\u306e\u54043\u3064\u306e\u30de\u30fc\u30ab\u30fc\uff08\u8a089\u3064\u306e\u30de\u30fc\u30ab\u30fc\uff09\u304b\u3089\u59cb\u307e\u308a\u3001Applied Biosystems\u2122 GlobalFiler PCR Amplification Kit<\/a>\u306e\u65b0\u3057\u304f\u5c0e\u5165\u3055\u308c\u305f20\u30b3\u30a2CODIS\u907a\u4f1d\u5b50\u5ea77<\/sup>\u3078\u3068\u3001\u30d7\u30ed\u30d5\u30a1\u30a4\u30ea\u30f3\u30b0\u6a5f\u80fd\u304c\u8b58\u5225\u529b\uff08PoD\uff09\u3067\u5411\u4e0a\u3057\u3001\u56fd\u969b\u7684\u306a\u30c7\u30fc\u30bf\u5171\u6709\u306e\u53d6\u308a\u7d44\u307f\u304c\u62e1\u5927\u3057\u307e\u3057\u305f\u3002
\n\u73fe\u5728\u3001\u5bfe\u7acb\u907a\u4f1d\u5b50\u306e\u5206\u985e\u306f\u5358\u7d14\u306a\u547d\u540d\u898f\u52478-10<\/sup>\u306b\u5f93\u3063\u3066\u3001\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\uff08CE\uff09\u306b\u3088\u308bPCR\u30d9\u30fc\u30b9\u306e\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u30b5\u30a4\u30b8\u30f3\u30b0\u306b\u3088\u3063\u3066\u8b58\u5225\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u898f\u5247\u306f\u3001CE\u30b7\u30b9\u30c6\u30e0\u306b\u3088\u3063\u3066\u7b97\u51fa\u3055\u308c\u308b\u30b5\u30a4\u30ba\u306e\u5909\u52d5\u306b\u57fa\u3065\u3044\u3066\u3044\u308b\u305f\u3081\u3001\u3042\u308b\u5bfe\u7acb\u907a\u4f1d\u5b50\u306e\u5869\u57fa\u914d\u5217\u306e\u9055\u3044\u306f\u8003\u616e\u3055\u308c\u3066\u3044\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u3053\u308c\u3089\u306e\u30b5\u30a4\u30ba\u30d9\u30fc\u30b9\u306e\u5bfe\u7acb\u907a\u4f1d\u5b50\u306ePoD\u306f\u3001\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u76ee\u7684\u3067\u9664\u5916\u307e\u305f\u306f\u5305\u542b\u306b\u95a2\u3059\u308b\u60c5\u5831\u3092\u63d0\u4f9b\u3059\u308b\u306e\u306b\u5341\u5206\u3067\u3042\u308b\u3053\u3068\u304c\u8a3c\u660e\u3055\u308c\u3066\u304a\u308a\u3001Applied Biosystems\u2122 GlobalFiler PCR Amplification Kit\u3084Applied Biosystems\u2122 NGMDetect Amplification Kit\u306a\u3069\u306e\u5927\u898f\u6a21\u306a\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b97,11<\/sup>\u306e\u5c0e\u5165\u3068\u3068\u3082\u306b\u6210\u9577\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306e\u767b\u5834\u3068\u8ab2\u984c<\/h2>\n

\"\"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\uff08NGS\uff09<\/a>\u306e\u767b\u5834\u306f\u3001\u6cd5\u533b\u5b66\u7684\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u5206\u91ce\u306b\u65b0\u305f\u306a\u8996\u70b9\u3092\u52a0\u3048\u3001\u30ea\u30d4\u30fc\u30c8\u30e2\u30c1\u30fc\u30d5\u306e\u5869\u57fa\u914d\u5217\u3001\u3055\u3089\u306b\u591a\u304f\u306e\u30de\u30fc\u30ab\u30fc\u3092\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u5316\u3001\u30ea\u30d4\u30fc\u30c8\u30e2\u30c1\u30fc\u30d5\u306e\u5869\u57fa\u914d\u5217\u3068\u96a3\u63a5\u9818\u57df\u306e\u8fd1\u304f\u306e\u30d0\u30ea\u30a8\u30fc\u30b7\u30e7\u30f3\u3092\u5206\u6790\u3001\u304a\u3088\u3073\u52a3\u5316\u3057\u305f\u30b5\u30f3\u30d7\u30eb12\u201314<\/sup> \u306e\u5206\u6790\u304c\u53ef\u80fd\u3068\u306a\u308b\u70b9\u3067\u3001CE\u5206\u6790\u3088\u308a\u3082\u512a\u308c\u3066\u3044\u307e\u3059\u3002NGS\u30d9\u30fc\u30b9\u306eSTR\u30bf\u30a4\u30d4\u30f3\u30b0\u306e\u8a55\u4fa1\u306f\u3001Applied Biosystems\u2122 Precision ID GlobalFiler NGS STR Panel v2<\/a>\u306b\u542b\u307e\u308c\u308b\u3088\u308a\u591a\u304f\u306e\u907a\u4f1d\u5b50\u5ea7\u3068\u8ffd\u52a0\u306e\u914d\u5217\u60c5\u5831\u306e\u4e21\u65b9\u3092\u542b\u3081\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u6df7\u5408\u7269\u306e\u89e3\u50cf\u5ea6\u3092\u5411\u4e0a\u3055\u305b\u3001PoD12,15<\/sup> \u3092\u5411\u4e0a\u3055\u305b\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3001\u30b1\u30fc\u30b9\u30ef\u30fc\u30af\u3067\u5f79\u7acb\u3064\u53ef\u80fd\u6027\u304c\u3042\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002NGS\u306f\u3088\u308a\u5927\u304d\u306a\u898b\u5730\u3078\u306e\u6249\u3092\u958b\u304d\u307e\u3059\u304c\u3001STR\u907a\u4f1d\u5b50\u5ea7\u306b\u3069\u308c\u3060\u3051\u306e\u914d\u5217\u5909\u7570\u304c\u5b58\u5728\u3059\u308b\u304b\u304c\u5927\u304d\u306a\u8ab2\u984c\u3067\u3082\u3042\u308a\u307e\u3059\u3002<\/p>\n

Novroski et al.16<\/sup>\u306b\u3088\u308b\u6700\u8fd1\u306e\u7814\u7a76\u3067\u306f\u30014\u96c6\u56e3\uff08\u30a2\u30d5\u30ea\u30ab\u7cfb\u30a2\u30e1\u30ea\u30ab\u4eba\u3001\u767d\u4eba\u3001\u30d2\u30b9\u30d1\u30cb\u30c3\u30af\u3001\u304a\u3088\u3073\u4e2d\u56fd\u4eba\uff09\u306e700\u4eba\u4ee5\u4e0a\u3092\u89e3\u6790\u3057\u3001\u7279\u306bD21S11\u3001D2S1338\u304a\u3088\u3073D12S391\u306b\u304a\u3044\u30664\u3064\u306e\u96c6\u56e3\u3059\u3079\u3066\u3067\u30ea\u30d4\u30fc\u30c8\u30e2\u30c1\u30fc\u30d5\u914d\u5217\u304b\u3089\u3088\u308a\u9ad8\u3044\u591a\u69d8\u6027\u304c\u89b3\u5bdf\u3055\u308c\u307e\u3057\u305f\u3002\u4e00\u65b9\u3001CSF1PO\u3001D10S1248\u3001TH01\u3001\u304a\u3088\u3073TPOX\u306f\u3001\u5404\u5bfe\u7acb\u907a\u4f1d\u5b50\u306b\u304a\u3051\u308b\u5869\u57fa\u914d\u5217\u3067\u306e\u591a\u69d8\u6027\u3092\u307b\u3068\u3093\u3069\u3001\u307e\u305f\u306f\u307e\u3063\u305f\u304f\u89b3\u5bdf\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3053\u306e\u30c7\u30fc\u30bf\u3092\u5b9f\u969b\u306b\u4f7f\u7528\u3059\u308b\u65b9\u6cd5\u3092\u7406\u89e3\u3059\u308b\u305f\u3081\u306b\u3001\u3055\u3089\u306b\u591a\u304f\u306e\u7814\u7a76\u304c\u9032\u884c\u4e2d\u3067\u3042\u308a\u3001\u3053\u308c\u3089\u306e\u767a\u898b\u3092\u7167\u5408\u3059\u308b\u305f\u3081\u306e\u65b0\u3057\u3044\u30ea\u30dd\u30b8\u30c8\u30eaPOPSEQ\u304c\u30ce\u30fc\u30b9\u30ab\u30ed\u30e9\u30a4\u30ca\u5dde\u7acb\u5927\u5b66\u306b\u3088\u3063\u3066\u7ba1\u7406\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\n\"\"
\n\u5f93\u6765\u306eCE\u30d9\u30fc\u30b9\u306e\u547d\u540d\u898f\u5247\u3067\u306f\u3001\u8ffd\u52a0\u306e\u30d0\u30ea\u30a8\u30fc\u30b7\u30e7\u30f3\u3092\u8003\u616e\u306b\u5165\u308c\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001ISFG\u306e\u7406\u4e8b\u4f1a\u306f\u3001NGS\u30d9\u30fc\u30b9\u306eSTR\u547d\u540d\u898f\u5247\u306b\u5fc5\u8981\u306a\u8003\u616e\u4e8b\u9805\u3092\u8a55\u4fa1\u3059\u308b\u305f\u3081\u306bDNA\u59d4\u54e1\u4f1a\u3092\u7acb\u3061\u4e0a\u3052\u307e\u3057\u305f\u3002\u6cd5\u533b\u5b66\u30b3\u30df\u30e5\u30cb\u30c6\u30a3\u306f\u3001CE\u30d9\u30fc\u30b9\u3068NGS\u30d9\u30fc\u30b9\u306e\u4e21\u65b9\u306eSTR\u30bf\u30a4\u30d4\u30f3\u30b0\u65b9\u6cd5\u304c\u7d99\u7d9a\u3057\u3066\u5171\u5b58\u3059\u308b\u3053\u3068\u3092\u671f\u5f85\u3057\u3066\u3044\u307e\u305917<\/sup>\u3002<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u30fb\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\uff08CE\uff09\u3092\u7528\u3044\u305fSTR\u89e3\u6790\u306f\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u30b4\u30fc\u30eb\u30c9\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u3067\u3059\u3002
\n\u30fb\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\uff08NGS\uff09\u306f\u6cd5\u533b\u5b66\u7684\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u306e\u5206\u91ce\u306b\u304a\u3044\u3066\u3082\u3088\u308a\u591a\u304f\u306e\u60c5\u5831\u3092\u5f97\u308b\u3053\u3068\u304c\u3067\u304d\u4eca\u5f8c\u3082\u671f\u5f85\u3055\u308c\u3066\u3044\u307e\u3059\u3002
\n\u30fb\u6cd5\u533b\u5b66\u30b3\u30df\u30e5\u30cb\u30c6\u30a3\u306f\u3001CE\u30d9\u30fc\u30b9\u3068NGS\u30d9\u30fc\u30b9\u306e\u4e21\u65b9\u306eSTR\u89e3\u6790\u6cd5\u304c\u7d99\u7d9a\u3059\u308b\u3053\u3068\u3092\u671f\u5f85\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u30d2\u30c8\u500b\u4eba\u8b58\u5225\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u306e\u8a73\u7d30\u306f\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u53c2\u8003\u6587\u732e<\/strong>
\n1. P. Gill, A. J. Jeffreys, D. J. Werrett, Forensic application of DNA \u2018fingerprints\u2019, Nature 318 (6046) (1985) 577-9.
\n2. A. J. Jeffreys, V. Wilson, S. L. Thein, Individual-specific \u2018fingerprints\u2019 of human DNA, Nature 316 (6023) (1985) 76-9.
\n3. C. Puers, H.A. Hammond, L. Jin, C.T. Caskey, J.W. Schumm, Identification of repeat sequence heterogeneity at the polymorphic short tandem repeat locus HUMTH01[AATG]n and reassignment of alleles in population analysis by using a locus-specific allelic ladder, Am. J. Hum. Genet. 53 (1993) 953\u2013958.
\n4. P. Gill, C. Kimpton, E. D\u2019Aloja, J.F. Andersen, W. Bar, B. Brinkmann, et al., Report of the European DNA profiling group (EDNAP)\u2014towards standardisation of short tandem repeat (STR) loci, Forensic Sci. Int. 65 (1994) 51\u201359.
\n5. P. Gill, R. Sparkes, C. Kimpton, Development of guidelines to designate alleles using an STR multiplex system, Forensic Sci. Int. 89 (1997) 185\u2013197.
\n6. B. Budowle, T.R. Moretti, A.L. Baumstark, D.A. Defenbaugh, K.M. Keys, Population data on the thirteen CODIS core short tandem repeat loci in African Americans, U.S., Caucasians, Hispanics, Bahamians, Jamaicans, and Trinidadians, J. Forensic Sci. 44 (1999) 1277\u20131286.
\n7. D. R. Hares, Selection and implementation of expanded CODIS core loci in the United States, Forensic Sci Int Genet 17 (2015) 33-4.
\n8. W. B\u00e4r, B. Brinkmann, B. Budowle, A. Carracedo, P. Gill, P. Lincoln, et al., DNA recommendations. Further report of the DNA Commission of the ISFH regarding the use of short tandem repeat systems. International Society for Forensic Haemogenetics, Int. J. Legal Med. 110 (1997) 175\u2013176.
\n9. J.M. Butler, Genetics and genomics of core short tandem repeat loci used in human identity testing, J. Forensic Sci. 51 (2006) 253\u2013265.
\n10. P.M. Schneider, Scientific standards for studies in forensic genetics, Forensic Sci. Int. 165 (2007) 238\u2013243.
\n11. P.M. Schneider, Expansion of the European Standard Set of DNA database loci\u2014 the current situation, Profiles DNA (2009) 6\u20137.
\n12. C. Borsting, N. Morling, Next generation sequencing and its applications in forensic genetics, Forensic Sci. Int. Genet. 18 (2015) 78\u201389.
\n13. W. Parson, G. Huber, L. Moreno, M.B. Madel, M.D. Brandhagen, S. Nagl, et al., Massively parallel sequencing of complete mitochondrial genomes from hair shaft samples, Forensic Sci. Int. Genet. 15 (2015) 8\u201315.
\n14. M. Eduardoff, C. Santos, M. de la Puente, T.E. Gross, M. Fondevila, C. Strobl, et al., Inter-laboratory evaluation of SNP-based forensic identification by massively parallel sequencing using the Ion PGM, Forensic Sci. Int. Genet. 17 (2015) 110\u2013 121.
\n15. K.B. Gettings, R.A. Aponte, P.M. Vallone, J.M. Butler, STR allele sequence variation: current knowledge and future issues, Forensic Sci. Int. Genet. 18 (2015) 118\u2013130.
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\n17. W. Parson et al. Massively parallel sequencing of forensic STRs: Considerations of the DNA commission of the International Society for Forensic Genetics (ISFG) on minimal nomenclature requirements Forensic Science International: Genetics 22 (2016) 54\u201363<\/p>\n

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