{"id":111,"date":"2015-10-13T00:00:00","date_gmt":"2015-10-13T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic9\/"},"modified":"2025-04-17T15:15:29","modified_gmt":"2025-04-17T06:15:29","slug":"qpcr-basic9","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/qpcr-basic9\/","title":{"rendered":"PCR\u5897\u5e45\u7523\u7269\u3092\u201c\u304d\u3063\u3061\u308a\u201d\u691c\u51fa\u3059\u308b\uff5c\u4eca\u3053\u305d\u672c\u6c17\u3067\u5fb9\u5e95\u7406\u89e3! \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u8b1b\u5ea7  \u7b2c9\u56de"},"content":{"rendered":"<p>\u4eca\u56de\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u7523\u7269\u306e\u691c\u51fa\u306b\u3064\u304b\u3046\u30b1\u30df\u30b9\u30c8\u30ea\u306b\u306f\u3069\u3093\u306a\u3082\u306e\u304c\u3042\u308b\u306e\u304b\u3001\u898b\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n<p>\u5f0a\u793e\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3067\u306f\u3001\u300c<strong>SYBR\u00ae Green I<\/strong>\u300d \u3001\u300c<strong>Taqman\u00ae\u30d7\u30ed\u30fc\u30d6<\/strong>\u300d\u3068\u3044\u30462\u7a2e\u985e\u306e\u30b1\u30df\u30b9\u30c8\u30ea\u3092\u7528\u3044\u305f\u691c\u51fa\u65b9\u6cd5\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2>SYBR\u00ae Green I<\/h2>\n<p>SYBR\u00ae Green I\u306f\u3001<strong>2\u672c\u9396DNA\u306b\u5165\u308a\u8fbc\u3080\u3068\u86cd\u5149\u3092\u767a\u3059\u308b\u86cd\u5149\u8272\u7d20<\/strong>\u3067\u3059\u3002\u3053\u306e\u86cd\u5149\u8272\u7d20\u306b\u306f\u30012\u672c\u9396DNA\u914d\u5217\u306e\u307f\u3092\u6a19\u8b58\u3059\u308b\u7279\u7570\u6027\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u3084\u975e\u7279\u7570\u7684\u306aPCR\u7523\u7269\u3068\u3044\u3063\u305f\u526f\u7523\u7269\u306b\u3082\u7d50\u5408\u3057\u3001\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u3092\u793a\u3057\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\uff08\u56f3\uff09\u3002\u3053\u306e\u554f\u984c\u3092\u9632\u3050\u305f\u3081\u306b\u306f\u3001<strong>\u76ee\u7684\u306ePCR\u7523\u7269\u306e\u307f\u3092\u7279\u7570\u7684\u306b\u5897\u5e45\u3059\u308b\u3088\u3046<\/strong>\u3001<strong>\u30d7\u30e9\u30a4\u30de\u30fc\u8a2d\u8a08\u3068\u6fc3\u5ea6\u306e\u6761\u4ef6\u691c\u8a0e\u3092\u304a\u3053\u306a\u3046\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059<\/strong>\u3002<\/p>\n<div id=\"attachment_319\" style=\"width: 580px\" class=\"wp-caption aligncenter\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic9-fig.jpg\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-319\" class=\"size-full wp-image-319\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/qpcr-basic9-fig.jpg\" alt=\"qpcr-basic9-fig\" width=\"570\" height=\"283\" \/><\/a><p id=\"caption-attachment-319\" class=\"wp-caption-text\">\u56f3 SYBR\u00ae Green I\u306e\u7279\u5fb4\u3002\u7279\u7570\u7684\u306a\u30b1\u30df\u30b9\u30c8\u30ea\u3067\u306f\u306a\u3044\u306e\u3067\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306f\u614e\u91cd\u306b\u8a2d\u8a08\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3002<\/p><\/div>\n<h2>Taqman\u00ae\u30d7\u30ed\u30fc\u30d6<\/h2>\n<p>Taqman\u00ae\u30d7\u30ed\u30fc\u30d6\u306f20\uff5e30mer\u306e\u9577\u3055\u306e\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u3067\u3001<strong>\u76f8\u88dc\u7684\u306a\u914d\u5217\u3092\u7279\u7570\u7684\u306b\u8a8d\u8b58\u3057\u307e\u3059<\/strong>\uff085&#8217;\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u30a2\u30c3\u30bb\u30a4\uff09\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u3084\u975e\u7279\u7570\u7684\u306aPCR\u7523\u7269\u3068\u3044\u3063\u305f\u526f\u7523\u7269\u304c\u3067\u304d\u3066\u3057\u307e\u3063\u3066\u3082\u3001\u30d7\u30ed\u30fc\u30d6\u306e\u914d\u5217\u7279\u7570\u6027\u304c\u526f\u7523\u7269\u306e\u5f71\u97ff\u3092\u6700\u5c0f\u9650\u306b\u3057\u3066\u304f\u308c\u307e\u3059\u3002<\/p>\n\n\t<blockquote>\n\t\t\u4e21\u8005\u306e\u9055\u3044\u3092\u307e\u3068\u3081\u307e\u3057\u3087\u3046\u3002SYBR\u00ae Green \u306f\u30d7\u30e9\u30a4\u30de\u30fc\u3060\u3051\u3067\u914d\u5217\u3092\u8b58\u5225\u3059\u308b\u306e\u306b\u5bfe\u3057\u3001Taqman\u00ae\u30d7\u30ed\u30fc\u30d6\u6cd5\u306f\u30d7\u30e9\u30a4\u30de\u30fc\u3068Taqman\u00ae\u30d7\u30ed\u30fc\u30d6\u306e\u4e21\u65b9\u3067\u914d\u5217\u3092\u8a8d\u8b58\u3057\u307e\u3059\u3002Taqman\u00ae\u30d7\u30ed\u30fc\u30d6\u306f\u3001\u3088\u308a\u9ad8\u3044\u7279\u7570\u6027\u3067PCR\u7523\u7269\u3092\u691c\u51fa\u3067\u304d\u308b\u88fd\u54c1\u3068\u8a00\u3048\u308b\u306e\u3067\u3059\u3002\t<\/blockquote>\n\n\t<p style=\"text-align:center;font-weight:bold\"><\/p>\n\t\n\t\n<p>\u3068\u306f\u3044\u3048\u3001\u5b9f\u9a13\u306b\u3088\u3063\u3066\u306fSYBR\u00ae Green I\u3092\u3064\u304b\u3044\u305f\u3044\u3068\u3044\u3046\u65b9\u3082\u3044\u3089\u3063\u3057\u3083\u3044\u307e\u3059\u3002SYBR\u00ae Green I\u3092\u5229\u7528\u3059\u308b\u4e0a\u3067\u306e\u30a2\u30c9\u30d0\u30a4\u30b9\u306f\u3042\u308a\u307e\u3059\u304b\uff1f<\/p>\n<p>\n\t<blockquote>\n\t\tSYBR\u00ae Green I\u3092\u4f7f\u3046\u5834\u5408\u306b\u306f\u3001\u878d\u89e3\u66f2\u7dda\u89e3\u6790\u30d7\u30ed\u30b0\u30e9\u30e0\u3092\u5229\u7528\u3044\u305f\u3060\u304f\u3068\u5b89\u5fc3\u3067\u3059\u3002\u3053\u306e\u30d7\u30ed\u30af\u30e9\u30b0\u30e0\u306f\u5f0a\u793e\u306e\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u88c5\u7f6e\u306b\u4ed8\u5c5e\u3057\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u7279\u7570\u6027\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u3082\u3061\u308d\u3093\u3001PCR\u7523\u7269\u3092\u30b2\u30eb\u4e0a\u3067\u6cf3\u52d5\u3059\u308b\u3053\u3068\u3082\u3001\u78ba\u5b9f\u306b\u78ba\u8a8d\u3067\u304d\u308b\u65b9\u6cd5\u3067\u3059\u3002\t<\/blockquote>\n\n\t<p style=\"text-align:center;font-weight:bold\"><\/p>\n\t\n\t<br \/>\n\n\t<blockquote>\n\t\t\u30b5\u30f3\u30d7\u30eb\u5185\u306b\u975e\u5e38\u306b\u4f3c\u305f\u914d\u5217\u304c\u5b58\u5728\u3059\u308b\u5834\u5408\u3084\u3001\u30b9\u30d7\u30e9\u30a4\u30b7\u30f3\u30b0\u30d0\u30ea\u30a2\u30f3\u30c8\u3092\u533a\u5225\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u5834\u5408\u306b\u306f\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/en\/home\/life-science\/oligonucleotides-primers-probes-genes\/applied-biosystems-custom-primers-probes\/taqman-mgb-probe.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr9_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">Taqman\u00ae MGB\u30d7\u30ed\u30fc\u30d6<\/a>\u3092\u7528\u3044\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002\u3068\u304f\u306b\u3001SYBR\u00ae Green I\u3067\u826f\u3044\u7cfb\u304c\u69cb\u7bc9\u3067\u304d\u305a\u306b\u82e6\u52b4\u3055\u308c\u3066\u3044\u308b\u65b9\u306f\u3001\u305c\u3072\u4e00\u5ea6\u3001Taqman\u00ae\u30d7\u30ed\u30fc\u30d6\u3092\u8a66\u3057\u3066\u307f\u3066\u4e0b\u3055\u3044\u3002SYBR\u00ae Green I\u304b\u3089Taqman\u00ae MGB\u30d7\u30ed\u30fc\u30d6\u306b\u4e57\u308a\u63db\u3048\u308b\u3053\u3068\u3067\u3001\u7d50\u679c\u7684\u306b\u30b3\u30b9\u30c8\u3092\u5b89\u304f\u3067\u304d\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\t<\/blockquote>\n\n\t<p style=\"text-align:center;font-weight:bold\"><\/p>\n\t\n\t<\/p>\n<p>\u4ee5\u524d\u3001\u3053\u306e\u8b1b\u5ea7\u3067\u3054\u7d39\u4ecb\u3057\u305f<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/taqman-gene-expression.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr9_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">TaqMan\u00ae Gene Expression Assays<\/a>\u3067\u3082\u3001Taqman\u00ae MGB\u30d7\u30ed\u30fc\u30d6\u3092\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u30d7\u30ed\u30fc\u30d6\u306e\u9078\u629e\u306b\u3088\u3063\u3066\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u3084PCR\u306e\u6761\u4ef6\u691c\u8a0e\u3092\u3057\u306a\u304f\u3066\u3082\u78ba\u5b9f\u306a\u7d50\u679c\u3092\u5f97\u3089\u308c\u308b\u3068\u3044\u3046\u308f\u3051\u3067\u3059\u3002<\/p>\n<p>\u6b21\u56de\u306f\u3001\u5185\u5728\u6027\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306b\u3064\u3044\u3066\u8003\u3048\u307e\u3059\u3002<\/p>\n<h2>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30cf\u30f3\u30c9\u30d6\u30c3\u30af \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h2>\n<p>\u3053\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7406\u8ad6\u3084\u5b9f\u9a13\u30c7\u30b6\u30a4\u30f3\u306e\u8a2d\u8a08\u306a\u3069\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u57fa\u790e\u77e5\u8b58\u304c\u63b2\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u59cb\u3081\u305f\u3070\u304b\u308a\u306e\u65b9\u3084\u3053\u308c\u304b\u3089\u5b9f\u9a13\u3092\u8003\u3048\u3066\u3044\u308b\u65b9\u306b\u3046\u3063\u3066\u3064\u3051\u306e\u30cf\u30f3\u30c9\u30d6\u30c3\u30af\u3067\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u3057\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/real-time-pcr-handbook-form.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr9_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<h2>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0 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rel=\"noopener\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u7523\u7269\u306e\u691c\u51fa\u306b\u3064\u304b\u3046\u30b1\u30df\u30b9\u30c8\u30ea\u306b\u306f\u3069\u3093\u306a\u3082\u306e\u304c\u3042\u308b\u306e\u3067\u3057\u3087\u3046\u3002\u305d\u306e\u9078\u629e\u306b\u3088\u3063\u3066\u3001\u691c\u51fa\u306e\u7cbe\u5ea6\u3084\u52b9\u7387\u304c\u7570\u306a\u3063\u3066\u304d\u307e\u3059\u3002\u5f0a\u793e\u304a\u3059\u3059\u3081\u306e\u30d7\u30ed\u30fc\u30d6\u3082\u308f\u304b\u308a\u3084\u3059\u304f\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n","protected":false},"author":988,"featured_media":2153,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[7],"division":[],"class_list":{"0":"post-111","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-qpcr","9":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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