{"id":11335,"date":"2022-04-04T11:55:27","date_gmt":"2022-04-04T02:55:27","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=11335"},"modified":"2024-09-18T14:12:05","modified_gmt":"2024-09-18T05:12:05","slug":"gene-editing_bid_ts_2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_2\/","title":{"rendered":"\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c2\u56de\u3000\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\uff5e"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_1\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de<\/a>\u306e\u30d6\u30ed\u30b0\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u539f\u7406\u3092\u5b66\u3093\u3060A\u3055\u3093\u306f\u3001\u6b21\u306b\u5148\u8f29\u304b\u3089\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3\u306b\u304a\u3051\u308b\u6ce8\u610f\u70b9\u3084\u65b9\u6cd5\u3092\u5b66\u3076\u3053\u3068\u306b\u306a\u308a\u307e\u3057\u305f\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-13986\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-300x160.png\" alt=\"\" width=\"500\" height=\"267\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-300x160.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-1024x546.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-768x410.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-1536x819.png 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01.png 1787w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/p>\n<h2>\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u65b9\u6cd5<\/h2>\n<p>\u307e\u305a\u3001\u30ac\u30a4\u30c9RNA\u306e\u3046\u3061\u3001\u6a19\u8b58\u907a\u4f1d\u5b50\u306b\u5bfe\u3057\u3066\u30c7\u30b6\u30a4\u30f3\u304c\u5fc5\u8981\u306a\u306e\u306fcrRNA\u90e8\u5206\u306e\u307f\u3067\u3059\u3002\u4e0b\u6d41\u306etracrRNA\u90e8\u5206\u306f\u5171\u901a\u3067\u3059\u306e\u3067\u3001PAM\u914d\u5217\u306e\u4e0a\u6d41\u306b\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u907a\u4f1d\u5b50\u306b\u76f8\u88dc\u7684\u306acrRNA\u914d\u5217\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002A\u3055\u3093\u306f\u3001\u65e9\u901f\u30ac\u30a4\u30c9RNA\u306ecrRNA\u90e8\u5206\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u3088\u3046\u3068\u8a66\u307f\u3066\u3044\u307e\u3059\u3002<\/p>\n<div id=\"attachment_14406\" style=\"width: 825px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14406\" class=\"size-full wp-image-14406\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-02.png\" alt=\"\u30ac\u30a4\u30c9RNA\u6a21\u5f0f\u56f3\" width=\"815\" height=\"377\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-02.png 815w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-02-300x139.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-02-768x355.png 768w\" sizes=\"auto, (max-width: 815px) 100vw, 815px\" \/><p id=\"caption-attachment-14406\" class=\"wp-caption-text\">\u56f31\uff1a\u30ac\u30a4\u30c9RNA\u6a21\u5f0f\u56f3<\/p><\/div>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\nSpCas9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u3092\u4f7f\u3046\u5834\u5408\u3001PAM\u914d\u5217\u306fNGG\u3060\u304b\u3089\u3001\u3053\u306e\u4e0a\u6d4119\uff5e20\u5869\u57fa\u3067crRNA\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u3093\u3067\u3059\u3088\u306d\u3002\u3067\u3001\u3053\u306ecrRNA\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u4ee5\u5916\u306e\u90e8\u5206\u306b\u7d50\u5408\u3057\u3061\u3083\u3046\u3068\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u306b\u306a\u308b\u304b\u3089\u2026<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u60a9\u3093\u3067\u308b\u306d\u3002crRNA\u3092\u81ea\u5206\u3067\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u306e\u306f\u7d50\u69cb\u5927\u5909\u3060\u3088\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u3042\u3001\u5148\u8f29\u3002crRNA\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u4ee5\u5916\u306e\u90e8\u5206\u306b\u7d50\u5408\u3057\u3061\u3083\u3046\u3068\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u304c\u51fa\u3061\u3083\u3044\u307e\u3059\u3088\u306d\u3002\u7279\u7570\u6027\u306e\u9ad8\u3044crRNA\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u306b\u306f\u3001\u3069\u3046\u3059\u308c\u3070\u3044\u3044\u3093\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n<p>\u307e\u305a\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3068\u306f\u3001\u76ee\u7684\u306e\u90e8\u4f4d\u4ee5\u5916\u306b\u5909\u7570\u304c\u633f\u5165\u3055\u308c\u3066\u3057\u307e\u3046\u3053\u3068\u3067\u3059\u3002\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3067\u5909\u7570\u304c\u5165\u3063\u305f\u5834\u5408\u3001\u78ba\u8a8d\u3055\u308c\u305f\u8868\u73fe\u578b\u304c\u3001\u76ee\u7684\u306e\u5909\u7570\u7531\u6765\u306e\u3082\u306e\u306a\u306e\u304b\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u7531\u6765\u306e\u3082\u306e\u306a\u306e\u304b\u3001\u5224\u65ad\u304c\u96e3\u3057\u304f\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u6975\u529b\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u9632\u3050\u5de5\u592b\u304c\u5fc5\u8981\u3067\u3059\u3002\u305f\u3068\u3048\u3070\u3001Cas9\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7528\u3044\u305f\u5b9f\u9a13\u7cfb\u3092\u69cb\u7bc9\u3059\u308b\u3053\u3068\u3082\u52b9\u679c\u7684\u3067\u3059\u304c\uff08<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_3\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c3\u56de<\/a>\u3067\u8a73\u3057\u304f\u8aac\u660e\u3057\u3066\u3044\u307e\u3059\uff09\u3001\u305d\u3082\u305d\u3082crRNA\u306e\u7279\u7570\u6027\u304c\u9ad8\u304f\u306a\u3044\u3068\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u4ee5\u5916\u306e\u3068\u3053\u308d\u306b\u7d50\u5408\u3057\u3066\u3057\u307e\u3044\u307e\u3059\u3002<\/p>\n<p>\u3067\u306f\u3001\u7279\u7570\u6027\u306e\u9ad8\u3044crRNA\u3068\u306f\u3069\u306e\u3088\u3046\u306a\u914d\u5217\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u7279\u7570\u6027\u306e\u9ad8\u3044crRNA\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u30dd\u30a4\u30f3\u30c8\u306f\u30012\u3064\u3042\u308b\u3088<\/p>\n<p><b>\uff1c\u7279\u7570\u6027\u306e\u9ad8\u3044crRNA\u306e\u6761\u4ef6\uff1e<\/b><\/p>\n<ul>\n<li><b>Seed\u914d\u5217\uff08PAM\u914d\u5217\u306e\u4e0a\u6d4112\u5869\u57fa\uff09\u306e\u7279\u7570\u6027\u3092\u9ad8\u304f\u3059\u308b\u3053\u3068<\/b><\/li>\n<li><b>crRNA\u306e\u914d\u5217\u304c\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u4ee5\u5916\u306e\u914d\u5217\u30683\u3064\u4ee5\u4e0a\u7570\u306a\u308b\u5869\u57fa\u3092\u6301\u3064\u3053\u3068<\/b><\/li>\n<\/ul>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-11338\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/03\/Image1-2-1024x371.jpg\" alt=\"\" width=\"760\" height=\"275\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/03\/Image1-2-1024x371.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/03\/Image1-2-300x109.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/03\/Image1-2-768x278.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/03\/Image1-2-1536x557.jpg 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/03\/Image1-2.jpg 1959w\" sizes=\"auto, (max-width: 760px) 100vw, 760px\" \/>\u3064\u307e\u308a\u3001crRNA\u306e\u3046\u3061\u7279\u306bSeed\u914d\u5217\u306e\u7279\u7570\u6027\u3092\u9ad8\u304f\u3057\u3066\u3001\u304b\u3064\u3001crRNA\u304c\u9593\u9055\u3048\u3066\u5225\u306e\u5834\u6240\u306b\u7d50\u5408\u3057\u306a\u3044\u3088\u3046\u306b\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3068\u306a\u308a\u3046\u308b\u914d\u5217\u3068crRNA\u914d\u5217\u306e\u9593\u306b\u306f\u30013\u3064\u4ee5\u4e0a\u306e\u7570\u306a\u308b\u5869\u57fa\u304c\u3042\u308b\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u3053\u3046\u3044\u3063\u305f\u914d\u5217\u3092\u81ea\u5206\u3067\u63a2\u3059\u306e\u306f\u5927\u5909\u3067\u3059\u3088\u306d\u3002\u305d\u306e\u305f\u3081\u3001\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u30c4\u30fc\u30eb\u306b\u3001\u30c7\u30b6\u30a4\u30f3\u3092\u4efb\u305b\u3066\u3057\u307e\u3044\u307e\u3057\u3087\u3046\u3002<\/p>\n<h2>\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7528\u306e\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3\u30c4\u30fc\u30eb\u306e\u4f7f\u3044\u65b9<\/h2>\n<p>\u3055\u3066\u3001A\u3055\u3093\u306e\u5b9f\u9a13\u3067\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u306b\u3088\u308b\u30e9\u30f3\u30c0\u30e0\u5909\u7570\u3092\u8d77\u3053\u3057\u3001\u300c\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u7834\u58ca\u300d\u3059\u308b\u3053\u3068\u304c\u76ee\u7684\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u3053\u3053\u3067\u306f\u30b2\u30ce\u30e0\u7de8\u96c6\u306e<b>\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u5b9f\u9a13\u7528\u306e\u30ac\u30a4RNA\u30c7\u30b6\u30a4\u30f3\u65b9\u6cd5<\/b>\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\u30ce\u30c3\u30af\u30a4\u30f3\u7528\u306e\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3\u65b9\u6cd5\u306b\u3064\u3044\u3066\u306f\u3001\u7b2c7\u56de\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u3068\u6ce8\u6587\u306f\u3001\u30aa\u30f3\u30e9\u30a4\u30f3\u3067\u884c\u3046\u3088<\/p>\n<p><b>\uff1c\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7528\u306e\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3\u3068\u6ce8\u6587\u65b9\u6cd5\uff1e<\/b><br \/>\n\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\uff06\u6ce8\u6587\u306b\u306f\u3001TrueDesign Genome Editor\u3092\u7528\u3044\u307e\u3059\u3002\u3053\u306e\u30c7\u30b6\u30a4\u30f3\u30c4\u30fc\u30eb\u306f\u3001PAM\u914d\u5217\u306a\u3069crRNA\u30c7\u30b6\u30a4\u30f3\u306e\u57fa\u672c\u306b\u6cbf\u3044\u306a\u304c\u3089\u3001\u4e0a\u8ff0\u306e\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u9632\u30502\u3064\u306e\u30dd\u30a4\u30f3\u30c8\u304c\u7df4\u308a\u8fbc\u307e\u308c\u305f\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u3067\u3001\u7c21\u5358\u306b\u30c7\u30b6\u30a4\u30f3\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u307e\u305a\u5f53\u793e\u306e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home.html?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30db\u30fc\u30e0\u30da\u30fc\u30b8<\/a>\u306b\u30a2\u30af\u30bb\u30b9\u3057\u3001\u53f3\u4e0a\u306e\u300c\u30b5\u30a4\u30f3\u30a4\u30f3\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3001\u300cConnect Your Lab\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14407\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-04.png\" alt=\"\" width=\"537\" height=\"466\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-04.png 537w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-04-300x260.png 300w\" sizes=\"auto, (max-width: 537px) 100vw, 537px\" \/>\u30b5\u30a4\u30f3\u30a4\u30f3\u304c\u6c42\u3081\u3089\u308c\u307e\u3059\u306e\u3067\u3001\u3059\u3067\u306b\u767b\u9332\u6e08\u307f\u306e\u5834\u5408\u306f\u3001\u30ed\u30b0\u30a4\u30f3\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u30a2\u30ab\u30a6\u30f3\u30c8\u3092\u304a\u6301\u3061\u3067\u306a\u3044\u5834\u5408\u306f\u3001\u53f3\u5074\u306e\u300c\u30a2\u30ab\u30a6\u30f3\u30c8\u3092\u767b\u9332\u3059\u308b\u300d\u304b\u3089\u767b\u9332\u3057\u3066\u304f\u3060\u3055\u3044\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14408\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-05.png\" alt=\"\" width=\"1312\" height=\"570\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-05.png 1312w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-05-300x130.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-05-1024x445.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-05-768x334.png 768w\" sizes=\"auto, (max-width: 1312px) 100vw, 1312px\" \/>Thermo Fisher Connect\u2122 Platform\u306e\u30da\u30fc\u30b8\u304c\u958b\u304d\u307e\u3059\u306e\u3067\u3001\u5de6\u4e0b\u306e\u300cAll apps\u300d\u304b\u3089\u300cTrueDesign Genome Editor\u300d\u3092\u63a2\u3057\u307e\u3057\u3087\u3046\u3002\u3082\u3057\u898b\u5f53\u305f\u3089\u306a\u3044\u3068\u304d\u306f\u3001\u300cAll apps\u300d\u3068\u66f8\u304b\u308c\u3066\u3044\u308b\u53f3\u6a2a\u306e\u300cFilter by\u300d\u3067\u300cSynthetic Biology\u300d\u3092\u9078\u3076\u3068\u51fa\u3066\u304d\u307e\u3059\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14409\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-06.png\" alt=\"\" width=\"547\" height=\"399\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-06.png 547w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-06-300x219.png 300w\" sizes=\"auto, (max-width: 547px) 100vw, 547px\" \/><\/p>\n<p>TrueDesign Genome Editor\u3092\u958b\u304f\u3068\u3001\u4e0b\u56f3\u306e\u3088\u3046\u306a\u30da\u30fc\u30b8\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14413\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-07.png\" alt=\"\" width=\"708\" height=\"316\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-07.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-07-300x134.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>\u307e\u305a\u306f\u30011. Select a gene editing experiment\u3067\u3001\u5b9f\u65bd\u4e88\u5b9a\u306e\u5b9f\u9a13\u3092\u9078\u3073\u307e\u3059\u3002<br \/>\n\u4eca\u56de\u3001A\u3055\u3093\u306f\u300c\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u5b9f\u9a13\u300d\u3092\u884c\u3046\u4e88\u5b9a\u306a\u306e\u3067\u3001\u3053\u306e\u30d6\u30ed\u30b0\u3067\u3082\u4e00\u756a\u5de6\u306e\u300cGene Knockout\u300d\u3092\u9078\u3073\u307e\u3059\u3002\u3053\u306e\u9078\u629e\u80a2\u4ee5\u5916\u306e\u9805\u76ee\u306f\u3001\u3059\u3079\u3066\u300c\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u300d\u3067\u9078\u3076\u9805\u76ee\u3067\u3059\u3002\u30ce\u30c3\u30af\u30a4\u30f3\u5b9f\u9a13\u306b\u3064\u3044\u3066\u306f\u3001\u7b2c7\u56de\u3067\u6539\u3081\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>Gene Knockout\u3092\u9078\u3076\u3068\u3001\u5de6\u5074\u306e\u300c1\u300d\u306e\u3068\u3053\u308d\u306b\u300cGene Knockout\u300d\u3068\u5165\u308a\u3001\u300cNext\u300d\u30dc\u30bf\u30f3\u304c\u30a2\u30af\u30c6\u30a3\u30d6\u306b\u306a\u308a\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14410\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-08.png\" alt=\"\" width=\"708\" height=\"246\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-08.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-08-300x104.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>\u300cNext\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u300c2. Select Species\u300d\u3067\u306f\u751f\u7269\u7a2e\u3092\u9078\u629e\u3057\u307e\u3059\u3002\u3053\u306e\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3\u30c4\u30fc\u30eb\u3067\u306f\u3001\u3053\u3053\u306b\u8868\u793a\u3055\u308c\u3066\u3044\u308b\u4ee5\u5916\u306e\u751f\u7269\u7a2e\u306f\u30c7\u30b6\u30a4\u30f3\u304c\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14411\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-09.png\" alt=\"\" width=\"708\" height=\"188\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-09.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-09-300x80.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>A\u3055\u3093\u306f\u300cHuman\u300d\u3067\u884c\u3046\u4e88\u5b9a\u306a\u306e\u3067\u3001\u3053\u3053\u3067\u306f\u300cHuman\u300d\u3092\u9078\u629e\u3057\u3001\u300cNext\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u307e\u3059\u3002<\/p>\n<p>\u300c3. Select Input Type\u300d\u3067\u306f\u3001\u907a\u4f1d\u5b50\u306e\u691c\u7d22\u306e\u4ed5\u65b9\u3092\u9078\u3073\u307e\u3059\u3002\u907a\u4f1d\u5b50\u540d\u304b\u3089\u691c\u7d22\u3057\u305f\u3044\u3068\u304d\u306f\u300cGene Symbol\u300d\u3001\u914d\u5217\u306e\u3068\u304d\u306f\u300cDNA Sequence\u300d\u3001\u67d3\u8272\u4f53\u306e\u30ed\u30b1\u30fc\u30b7\u30e7\u30f3\u304b\u3089\u691c\u7d22\u3059\u308b\u3068\u304d\u306f\u300cChromosome Locus\u300d\u3092\u9078\u3093\u3067\u304f\u3060\u3055\u3044\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14412\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-10.png\" alt=\"\" width=\"408\" height=\"176\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-10.png 408w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-10-300x129.png 300w\" sizes=\"auto, (max-width: 408px) 100vw, 408px\" \/><\/p>\n<p>\u3053\u3053\u3067\u306f\u3001\u591a\u304f\u306e\u4eba\u304c\u9078\u3070\u308c\u308b\u3067\u3042\u308d\u3046\u300cGene Symbol\u300d\u3092\u9078\u629e\u3057\u307e\u3059\u3002<\/p>\n<p>Gene Symbol\u3068\u3057\u3066\u3001\u8a66\u3057\u306b\u300cKLF4\u300d\u3068\u5165\u308c\u3066\u307f\u308b\u3068\u3001\u9078\u629e\u80a2\u304c\u8868\u793a\u3055\u308c\u307e\u3057\u305f\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14418\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-11.png\" alt=\"\" width=\"708\" height=\"176\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-11.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-11-300x75.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>\u8fd1\u3044\u907a\u4f1d\u5b50\u540d\u304c\u3042\u308b\u5834\u5408\u3001\u3053\u306e\u3088\u3046\u306b\u9078\u629e\u80a2\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u3057\u305f\u3044\u907a\u4f1d\u5b50\u3092\u9078\u3073\u3001\u300cSearch Gene\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u307e\u3057\u3087\u3046\u3002\u3053\u3053\u3067\u306f\u4e0a\u306e\u9078\u629e\u80a2\u3092\u9078\u3073\u307e\u3059\u3002<\/p>\n<p>\u300c5. Select one of the following xx transcript(s)\u300d\u3067\u306f\u3001\u305d\u306e\u907a\u4f1d\u5b50\u306b\u30d0\u30ea\u30a2\u30f3\u30c8\u304c\u3042\u308b\u5834\u5408\u3001\u3044\u305a\u308c\u304b\u3092\u9078\u3070\u306a\u304f\u3066\u306f\u306a\u308a\u307e\u305b\u3093\u3002\u300cTranscript ID\u300d\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001NCBI\u306e\u30da\u30fc\u30b8\u306b\u98db\u3093\u3067\u3001\u8a73\u7d30\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14414\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-12.png\" alt=\"\" width=\"708\" height=\"156\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-12.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-12-300x66.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>\u3053\u3053\u3067\u306f\u4e00\u4f8b\u3068\u3057\u3066\u3001\u4e0a\u306e\u907a\u4f1d\u5b50\u3092\u9078\u3073\u307e\u3059\u3002Select\u306b\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u3001\u300cNext\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u4e0b\u56f3\u306e\u3088\u3046\u306a\u753b\u9762\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002\u307e\u305a\u306f\u4e0a\u534a\u5206\u304b\u3089\u3054\u8aac\u660e\u3057\u307e\u3057\u3087\u3046\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14415\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-13.png\" alt=\"\" width=\"708\" height=\"177\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-13.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-13-300x75.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>\u4e00\u90e8\u62e1\u5927\u3059\u308b\u3068\u3001\u4e0b\u56f3\u306e\u3088\u3046\u306b\u306a\u308a\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14416\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-14.png\" alt=\"\" width=\"708\" height=\"277\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-14.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-14-300x117.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>\u6700\u4e0a\u6bb5\u306e\u56f3\u306f\u907a\u4f1d\u5b50\u30de\u30c3\u30d7\u3067\u3001Intron\u3001Exon\u3001UTR\u3001Flanking sequence\u306b\u5206\u304b\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u308c\u305e\u308c\u306e\u8272\u5206\u3051\u306f\u4e0b\u8a18\u306e\u3068\u304a\u308a\u3067\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14417\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-15.png\" alt=\"\" width=\"401\" height=\"25\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-15.png 401w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-15-300x19.png 300w\" sizes=\"auto, (max-width: 401px) 100vw, 401px\" \/><\/p>\n<p>\u4e0a\u304b\u30892\u6bb5\u76ee\u306e\u56f3\u300cSequence Editor\u300d\u306f\u3001\u907a\u4f1d\u5b50\u30de\u30c3\u30d7\u306e\u9752\u3044\u56db\u89d2\u5185\u3092\u62e1\u5927\u3057\u305f\u56f3\u3067\u3001\u907a\u4f1d\u5b50\u914d\u5217\u3001\u30a2\u30df\u30ce\u9178\u914d\u5217\u304c\u66f8\u304b\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3067\u306f\u3001\u3053\u306e\u907a\u4f1d\u5b50\u306b\u5bfe\u3057\u3066\u30ac\u30a4\u30c9RNA\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<br \/>\n\u753b\u9762\u4e0b\u306e\u300cSelect a Knockout Strategy\u300d\u3092\u9078\u3093\u3067\u304f\u3060\u3055\u3044\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14419\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-16.png\" alt=\"\" width=\"708\" height=\"216\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-16.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-16-300x92.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>\u3053\u3053\u3067\u306f\u3001\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u306e\u65b9\u6cd5\u3092\u9078\u3079\u307e\u3059\u3002\u5404\u9078\u629e\u80a2\u306e\u610f\u5473\u306f\u4e0b\u8a18\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n<ul>\n<li>Frameshift indel\uff1a\u4f55\u3089\u304b\u306e\u5909\u7570\u3092\u5165\u308c\u3066\u3001\u30d5\u30ec\u30fc\u30e0\u30b7\u30d5\u30c8\u3092\u8d77\u3053\u3059\u65b9\u6cd5<\/li>\n<li>Insert stop codon\uff1a\u30b9\u30c8\u30c3\u30d7\u30b3\u30c9\u30f3\u3092\u5165\u308c\u3066\u3001\u5909\u7570\u4f53\u3092\u4f5c\u308b\u65b9\u6cd5<\/li>\n<li>Knockout enrichment\uff1a\u30bf\u30b0\u3092\u5165\u308c\u3066\u3001\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u7834\u58ca\u3059\u308b\u65b9\u6cd5<\/li>\n<\/ul>\n<p>\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u65b9\u6cd5\u304c\u7279\u306b\u6c7a\u307e\u3063\u3066\u3044\u306a\u3044\u5834\u5408\u306f\u3001\u300cFrameshift indel\u300d\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002\u3053\u306e\u65b9\u6cd5\u304c\u6700\u3082\u3088\u304f\u9078\u3070\u308c\u3066\u3044\u308b\u305f\u3081\u3067\u3059\u3002\u65b9\u6cd5\u3092\u9078\u629e\u3057\u305f\u3089\u3001\u300cNext\u300d\u3067\u6b21\u306b\u9032\u3093\u3067\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u6b21\u306b\u300cSelect a gRNA option\u300d\u3092\u9078\u629e\u3057\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14420\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-17.png\" alt=\"\" width=\"708\" height=\"294\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-17.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-17-300x125.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<ul>\n<li style=\"text-align: left\">Predesigned sgRNA\uff1a\u30d7\u30ec\u30c7\u30b6\u30a4\u30f3\u306e\u30ac\u30a4\u30c9RNA\u3092\u767a\u6ce8\u3057\u305f\u3044\u5834\u5408<\/li>\n<li style=\"text-align: left\">Custom gRNA \uff06 TALEN Design\uff1a\u30ab\u30b9\u30bf\u30e0\u3067\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u3044\u5834\u5408<\/li>\n<\/ul>\n<p>\u306a\u304a\u3001\u30ab\u30b9\u30bf\u30e0\u3068\u8a00\u3063\u3066\u3082\u3001\u3054\u81ea\u8eab\u3067\u72ec\u81ea\u306b\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u308f\u3051\u3067\u306f\u306a\u304f\u3001\u914d\u5217\u4e2d\u306e\u3069\u306e\u7b87\u6240\u306b\u5909\u7570\u3092\u5165\u308c\u305f\u3044\u304b\u3092\u6307\u5b9a\u3057\u3066\u3001\u305d\u306e\u4ed8\u8fd1\u3067\u30c7\u30b6\u30a4\u30f3\u3092\u3059\u308b\u9078\u629e\u80a2\u306b\u306a\u308a\u307e\u3059\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u3053\u3053\u3067\u306f\u3001\u591a\u304f\u306e\u65b9\u304c\u9078\u629e\u3059\u308b\u30d7\u30ec\u30c7\u30b6\u30a4\u30f3\u914d\u5217\u3001\u3064\u307e\u308a\u300cPredesigned sgRNA\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u9032\u3081\u307e\u3059\u3002<\/p>\n<p>\u30c7\u30b6\u30a4\u30f3\u4e2d\u306f\u3001\u4e0b\u56f3\u306e\u3088\u3046\u306a\u8868\u793a\u304c\u73fe\u308c\u307e\u3059\u3002\u305f\u3044\u3066\u3044\u306e\u5834\u5408\u30011\u5206\u4ee5\u5185\u306b\u30c7\u30b6\u30a4\u30f3\u304c\u5b8c\u4e86\u3057\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14421\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-18.png\" alt=\"\" width=\"550\" height=\"243\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-18.png 550w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-18-300x133.png 300w\" sizes=\"auto, (max-width: 550px) 100vw, 550px\" \/><\/p>\n<div style=\"background: #ffffff;border: 1px solid #A1A1A1;padding: 10px\"><span style=\"color: #ff00ff\">\uff5e\u30ef\u30f3\u30dd\u30a4\u30f3\u30c8\u30a2\u30c9\u30d0\u30a4\u30b9\uff5e<\/span><br \/>\nPredesigned sgRNA\u3067\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u3068\u304d\u3001\u300cNo Design\u300d\u3068\u8868\u793a\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\n\u3053\u308c\u306f\u3001PAM\u914d\u5217\u304c\u898b\u3064\u304b\u3089\u306a\u3044\u5834\u5408\u3084\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u591a\u3059\u304e\u308b\u306a\u3069\u306e\u7406\u7531\u3067\u3001\u8a2d\u8a08\u3067\u304d\u306a\u3044\u3068\u304d\u306b\u8868\u793a\u3055\u308c\u308b\u30e1\u30c3\u30bb\u30fc\u30b8\u3067\u3059\u3002<br \/>\n\u3053\u3046\u3044\u3063\u305f\u30b1\u30fc\u30b9\u3067\u306f\u3001\u300cCustom gRNA\u300d\u3092\u9078\u629e\u3057\u3066\u3001\u5909\u7570\u3092\u5165\u308c\u305f\u3044\u7b87\u6240\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u307f\u308b\u3068\u3001\u30c7\u30b6\u30a4\u30f3\u3067\u304d\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/div>\n<p>&nbsp;<\/p>\n<p>\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305fgRNA\u306f\u30014\u7a2e\u985e\u8868\u793a\u3055\u308c\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-14424 aligncenter\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-20.png\" alt=\"\" width=\"708\" height=\"311\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-20.png 708w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-20-300x132.png 300w\" sizes=\"auto, (max-width: 708px) 100vw, 708px\" \/><\/p>\n<p>\u305d\u308c\u305e\u308c\u306e\u9805\u76ee\u306b\u3064\u3044\u3066\u3001\u8aac\u660e\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"179\">CRISPR Target Sequence<\/td>\n<td width=\"378\">\u30ac\u30a4\u30c9RNA\u306e\u914d\u5217\u3067\u3059\u3002<br \/>\n\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u30da\u30fc\u30b8\u4e0a\u306eSequence Editor\u5185\u3067\u8a72\u5f53\u914d\u5217\u304c\u30cf\u30a4\u30e9\u30a4\u30c8\u3055\u308c\u307e\u3059\u3002<\/td>\n<\/tr>\n<tr>\n<td width=\"179\">PAM Site<\/td>\n<td width=\"378\">SqCas9\u306ePAM\u914d\u5217\u3067\u3059\u3002<br \/>\n\u203b\u3053\u306e\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u306fSqCas9\u306e\u914d\u5217\u3057\u304b\u30c7\u30b6\u30a4\u30f3\u304c\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n<tr>\n<td width=\"179\">Score\uff08\uff05\uff09<\/td>\n<td width=\"378\">\u30b9\u30b3\u30a2\u3067\u3059\u3002<br \/>\n\u30a2\u30eb\u30b4\u30ea\u30ba\u30e0\u306e\u5408\u81f4\u7387\u304c\u9ad8\u3044\u307b\u3069\u30b9\u30b3\u30a2\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u304c\u3001\u30b9\u30b3\u30a2\u304c\u9ad8\u3044\u304b\u3089\u3068\u8a00\u3063\u3066\u6d3b\u6027\u304c\u9ad8\u3044\u3068\u306f\u9650\u308a\u307e\u305b\u3093\u3002\u76ee\u5b89\u306e\u3072\u3068\u3064\u3068\u304a\u8003\u3048\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n<tr>\n<td width=\"179\">Off-Targets<\/td>\n<td width=\"378\">\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5f15\u304d\u8d77\u3053\u3059\u53ef\u80fd\u6027\u306e\u3042\u308b\u914d\u5217\u306e\u6570\u3067\u3059\u3002\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u5404\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u914d\u5217\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"size-medium wp-image-14425 aligncenter\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-21-300x185.png\" alt=\"\" width=\"300\" height=\"185\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-21-300x185.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-21.png 446w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/td>\n<\/tr>\n<tr>\n<td width=\"179\">Target Locus<\/td>\n<td width=\"378\">\u30ac\u30a4\u30c9RNA\u306e\u30b2\u30ce\u30e0\u4e0a\u306e\u5834\u6240\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Target Sequence\u306e\u53f3\u6a2a\u306b\u3064\u3044\u3066\u3044\u308b\u7dd1\u8272\u306e\u30c1\u30a7\u30c3\u30af\u306f\u3001\u8868\u793a\u3055\u308c\u305f4\u7a2e\u985e\u306e\u4e2d\u3067\u63a8\u5968\u3055\u308c\u308b\u914d\u5217\u3067\u3059\u3002\u7dd1\u8272\u306e\u30c1\u30a7\u30c3\u30af\u304c\u3064\u3044\u3066\u3044\u308b\u914d\u5217\u306e\u4e2d\u306b\u512a\u52a3\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3053\u306e\u4f8b\u3067\u306f3\u3064\u306e\u914d\u5217\u306b\u30c1\u30a7\u30c3\u30af\u304c\u3064\u3044\u3066\u3044\u307e\u3059\u304c\u30013\u3064\u306e\u914d\u5217\u304c\u540c\u3058\u304f\u3089\u3044\u63a8\u5968\u3055\u308c\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<div style=\"background: #ffffff;border: 1px solid #A1A1A1;padding: 10px\"><span style=\"color: #ff00ff\">\uff5e\u30ef\u30f3\u30dd\u30a4\u30f3\u30c8\u30a2\u30c9\u30d0\u30a4\u30b9\uff5e<\/span><br \/>\n\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u5b9f\u9a13\u306e\u5834\u5408\u3001\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305f\u8907\u6570\u306e\u30ac\u30a4\u30c9RNA\u306e\u4e2d\u3067\u304a\u3059\u3059\u3081\u306a\u306e\u306f\u3001\u300c\u306a\u308b\u3079\u304f\u907a\u4f1d\u5b50\u306e\u4e0a\u6d41\u300d\u3067\u3001\u300c\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u304c\u5c11\u306a\u304f\u300d\u3001\u300c\u30b9\u30b3\u30a2\u304c\u9ad8\u3044\u3082\u306e\u300d\u3067\u3059\u3002\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u5b9f\u9a13\u306e\u5834\u5408\u3001\u7279\u306b\u907a\u4f1d\u5b50\u306e\u4e0a\u6d41\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u306f\u91cd\u8981\u3067\u3059\u3002<br \/>\n\u907a\u4f1d\u5b50\u306e\u4e0b\u6d41\u306b\u30ac\u30a4\u30c9RNA\u3092\u8a2d\u8a08\u3057\u3066\u3001\u30e9\u30f3\u30c0\u30e0\u5909\u7570\u3092\u633f\u5165\u3057\u305f\u5834\u5408\u3001\u305d\u306e\u5909\u7570\u90e8\u4f4d\u307e\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u6d3b\u6027\u3092\u6301\u3063\u3066\u3057\u307e\u3044\u3001\u91ce\u751f\u578b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u540c\u3058\u6a5f\u80fd\u3092\u6301\u3064\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3067\u3059\u3002<br \/>\n\u3053\u306e\u30c4\u30fc\u30eb\u3067\u306f\u30011<sup>st<\/sup>\uff5e3<sup>rd<\/sup>\u30a8\u30af\u30bd\u30f3\u5185\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u308b\u3088\u3046\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u304c\u3001\u30ac\u30a4\u30c9RNA\u306e\u914d\u5217\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u5834\u6240\u3092\u78ba\u8a8d\u3057\u3001\u306a\u308b\u3079\u304f\u4e0a\u6d41\u306e\u914d\u5217\u3092\u9078\u629e\u3057\u307e\u3057\u3087\u3046\u3002<\/div>\n<p>&nbsp;<\/p>\n<p>\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u306f\u3001\u30ac\u30a4\u30c9RNA\u306f\u307e\u305a1\u7a2e\u985e\u3092\u8cfc\u5165\u3057\u3066\u8a66\u3059\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002\u6ce8\u6587\u3057\u305f\u3044\u30ac\u30a4\u30c9RNA\u306e\u3044\u305a\u308c\u304b\u3072\u3068\u3064\u306b\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u3066\u3001Next\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p><a id=\"jump\"><\/a>\u6ce8\u6587\u30da\u30fc\u30b8\u306b\u98db\u3073\u307e\u3059\u3002<a href=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-22-1.png\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-14468 size-full\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-22-1.png\" alt=\"\" width=\"1556\" height=\"725\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-22-1.png 1556w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-22-1-300x140.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-22-1-1024x477.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-22-1-768x358.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-22-1-1536x716.png 1536w\" sizes=\"auto, (max-width: 1556px) 100vw, 1556px\" \/><\/a><\/p>\n<p>\u3053\u306e\u30da\u30fc\u30b8\u306e\u9805\u76ee\u3067\u306f\u3001\u6ce8\u6587\u3059\u308b\u3082\u306e\u306b\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u5404\u9805\u76ee\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\uff1c\u5de6\u5217\uff1e<br \/>\n<b>\u30fb<\/b>CRISPR gRNA Format\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u3066\u304f\u3060\u3055\u3044\u3002\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30ac\u30a4\u30c9RNA\u3092\u30aa\u30fc\u30c0\u30fc\u3067\u304d\u307e\u3059\u3002\u5bb9\u91cf\u306f1.5 nmol\u306e\u307f\u3067\u3059\u3002<br \/>\n<b>\u30fb<\/b>Cas9 Format\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5916\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4ee3\u7406\u5e97\u304b\u3089\u3054\u6ce8\u6587\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002<br \/>\n<b>\u30fb<\/b>Cas9 Reagent\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5916\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4ee3\u7406\u5e97\u304b\u3089\u3054\u6ce8\u6587\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002<br \/>\n<b>\u30fb<\/b>Transfection Reagent\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5916\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u4ee3\u7406\u5e97\u304b\u3089\u3054\u6ce8\u6587\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002<br \/>\n\u203b\u300cCas9 Format\u300d\u300cCas9 Reagent\u300d\u300cTransfection Reagent\u300d\u306f\u3001\u65e5\u672c\u3067\u306f\u3053\u3053\u304b\u3089\u6ce8\u6587\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u3082\u3057\u30c1\u30a7\u30c3\u30af\u3092\u3057\u305f\u307e\u307eCart\u306b\u5165\u308c\u308b\u3068\u3001\u300c\u6ce8\u6587\u3067\u304d\u307e\u305b\u3093\u300d\u3068\u3044\u3046\u30a2\u30e9\u30fc\u30c8\u304c\u51fa\u307e\u3059\u306e\u3067\u3001\u3053\u3053\u3067\u5916\u3057\u3066\u304a\u3044\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\uff1c\u53f3\u5217\uff1e<br \/>\n<b>\u30fb<\/b>Primers\uff1a\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u308b\u3068\u3001\u30df\u30b9\u30de\u30c3\u30c1\u7279\u7570\u7684\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u5207\u65ad\u89e3\u6790\uff08<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_5\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\">\u7b2c5\u56de<\/a>\u53c2\u7167\uff09\u3092\u5b9f\u65bd\u3059\u308b\u3068\u304d\u306ePCR\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u6ce8\u6587\u3067\u304d\u307e\u3059\u3002\u5bb9\u91cf\u306f25 nmol\u306e\u307f\u3067\u3059\u3002<br \/>\n\u300cView\u300d\u3092\u30af\u30ea\u30c3\u30af\u3059\u308b\u3068\u3001\u30ac\u30a4\u30c9RNA\u306b\u5bfe\u3057\u3066\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305fForward Primer\u3068Reverse Primer\u306e\u914d\u5217\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002\u30c7\u30d5\u30a9\u30eb\u30c8\u3060\u30683\u3064\u3059\u3079\u3066\u306b\u30c1\u30a7\u30c3\u30af\u304c\u5165\u3063\u3066\u3044\u308b\u306e\u3067\u30011\u7a2e\u985e\u3067\u3088\u3044\u5834\u5408\u306f\u3001\u3069\u308c\u304b1\u3064\u3060\u3051\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n<p><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-14427\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-23.png\" alt=\"\" width=\"709\" height=\"160\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-23.png 709w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/04\/gene-editing_bid_ts_2-23-300x68.png 300w\" sizes=\"auto, (max-width: 709px) 100vw, 709px\" \/><\/p>\n<p>\u203b\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306b\u5bfe\u3059\u308bPrimer\u306f\u3053\u306e\u30b5\u30a4\u30c8\u3067\u306f\u6ce8\u6587\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n<p><b>\u30fb<\/b>Other products You May Need\uff1a\u3059\u3079\u3066\u30c1\u30a7\u30c3\u30af\u3092\u5916\u3057\u3066\u304f\u3060\u3055\u3044\u3002\u5fc5\u8981\u306a\u5834\u5408\u306f\u4ee3\u7406\u5e97\u304b\u3089\u3054\u6ce8\u6587\u3092\u304a\u9858\u3044\u3044\u305f\u3057\u307e\u3059\u3002<br \/>\n<b>\u30fb<\/b>Download Designs and Protocol\uff1a\u30af\u30ea\u30c3\u30af\u3057\u3001\u3054\u6ce8\u6587\u5185\u5bb9\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3068\u4fdd\u5b58\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002\u30b5\u30a4\u30c8\u3067\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u306a\u3069\u3082\u4fdd\u5b58\u3057\u3066\u304a\u3051\u308b\u305f\u3081\u3067\u3059\u3002<\/p>\n<p>\u30c1\u30a7\u30c3\u30af\u3092\u5165\u308c\u7d42\u3048\u305f\u3089\u3001\u300cAdd to Cart\u300d\u3092\u30af\u30ea\u30c3\u30af\u3057\u3066\u30ab\u30fc\u30c8\u306b\u5165\u308c\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>\u3053\u306e\u3088\u3046\u306b\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305fcrRNA\u3092\u7528\u3044\u3066\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u884c\u3046\u3053\u3068\u3067\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3092\u53ef\u80fd\u306a\u9650\u308a\u9632\u3050\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u304c\u30bc\u30ed\u306b\u306a\u308b\u308f\u3051\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u682a\u3092\u6a39\u7acb\u3057\u305f\u969b\u306f\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u3088\u308b\u5909\u7570\u3092\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046\u3002\u3053\u306e\u30c4\u30fc\u30eb\u306e\u300cOff-Targets\u300d\u3067\u8868\u793a\u3055\u308c\u305f\u914d\u5217\u4ed8\u8fd1\u304c\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u3092\u5f15\u304d\u8d77\u3053\u3059\u53ef\u80fd\u6027\u306e\u9ad8\u3044\u914d\u5217\u3067\u3059\u306e\u3067\u3001\u305d\u306e\u4ed8\u8fd1\u306b\u3064\u3044\u3066\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u306a\u3069\u3067\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5909\u7570\u304c\u5c0e\u5165\u3055\u308c\u3066\u3044\u306a\u3044\u3053\u3068\u3092\u78ba\u8a8d\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u7528\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u306e\u307e\u3068\u3081<\/h2>\n<p>\u624b\u4f5c\u696d\u3067\u30ac\u30a4\u30c9RNA\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u306e\u306f\u5927\u5909\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u3001TrueDesign Genome Editor\u306e\u3088\u3046\u306a\u30aa\u30f3\u30e9\u30a4\u30f3\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3092\u4f7f\u7528\u3057\u307e\u3057\u3087\u3046\u3002\u3053\u3046\u3044\u3063\u305f\u30c4\u30fc\u30eb\u306e\u4f7f\u7528\u306b\u3088\u308a\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u304c\u4f4e\u304f\u3001\u7279\u7570\u6027\u306e\u9ad8\u3044\u30ac\u30a4\u30c9RNA\u304c\u30c7\u30b6\u30a4\u30f3\u3067\u304d\u307e\u3059\u3002<\/p>\n<p><b>TrueDesign Genome Editor\u306e\u7279\u5fb4<\/b><\/p>\n<ul>\n<li>\u30d2\u30c8\u3001\u30de\u30a6\u30b9\u3001\u30e9\u30c3\u30c8\u3001\u30bc\u30d6\u30e9\u30d5\u30a3\u30c3\u30b7\u30e5\u3001\u7dda\u866b\u306b\u5bfe\u5fdc<\/li>\n<li>1st~3rd\u30a8\u30af\u30bd\u30f3\u5185\u306b\u30c7\u30b6\u30a4\u30f3\uff08\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u306e\u5834\u5408\uff09<\/li>\n<li>SpCas9\uff08\u5316\u81bf\u30ec\u30f3\u30b5\u7403\u83cc\u7531\u6765\uff09\u306e\u307f\u5bfe\u5fdc<\/li>\n<li>4\u7a2e\u985e\u306e\u30d7\u30ec\u30c7\u30b6\u30a4\u30f3\u30ac\u30a4\u30c9RNA\u914d\u5217\u304c\u8868\u793a\u3055\u308c\u3001\u30b9\u30b3\u30a2\u3084\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u6570\u304b\u3089\u9078\u629e\u53ef\u80fd<\/li>\n<li>\u305d\u308c\u305e\u308c\u306e\u30d7\u30ec\u30c7\u30b6\u30a4\u30f3\u30ac\u30a4\u30c9RNA\u306e\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u914d\u5217\u304c\u78ba\u8a8d\u53ef\u80fd<\/li>\n<li>\u30df\u30b9\u30de\u30c3\u30c1\u7279\u7570\u7684\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u5207\u65ad<\/li>\n<\/ul>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u30ac\u30a4\u30c9RNA\u304c\u30c7\u30b6\u30a4\u30f3\u3067\u304d\u307e\u3057\u305f\uff01\u3053\u308c\u3067\u57f9\u990a\u7d30\u80de\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u5b9f\u9a13\u304c\u3067\u304d\u307e\u3059\u306d<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u5f85\u3063\u3066\u3002\u305d\u306e\u524d\u306b<b>CIRSPR\/Cas9<\/b><b>\u306e<\/b><b>3<\/b><b>\u3064\u306e\u5b9f\u9a13\u7cfb<\/b>\u304b\u3089\u3061\u3083\u3093\u3068\u9078\u3093\u3060\uff1f<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n3\u3064\u306e\u5b9f\u9a13\u7cfb\u3063\u3066\uff1f<\/p>\n<p>\u3055\u3066\u3001\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u3068\u6ce8\u6587\u304c\u5b8c\u4e86\u3057\u305fA\u3055\u3093\u3067\u3059\u304c\u3001\u6b21\u306b3\u3064\u306e\u5b9f\u9a13\u7cfb\u3068\u3084\u3089\u3092\u8003\u3048\u306a\u304f\u3066\u306f\u3044\u3051\u306a\u3044\u3088\u3046\u3067\u3059\u3002\u6b21\u56de\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_3?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c3\u56de<\/a>\u3067\u306f\u3001\u3053\u306e3\u3064\u306e\u5b9f\u9a13\u7cfb\u306b\u3064\u3044\u3066\u8a73\u3057\u304f\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p><b>\u7565\u8a9e\u89e3\u8aac<\/b><br \/>\nCRISPR; Clusterd regularly interspaced short palindromic repeats<br \/>\nSpCas9R; Streptococcus pyogenes\u7531\u6765\u306eCas9<br \/>\nCas; CRISPR-associated protein<br \/>\nPAM; Protospacer adjacent motif<\/p>\n<p>\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u30b7\u30ea\u30fc\u30ba\uff1e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_1\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c1\u56de\u3000CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306e\u539f\u7406\uff5e<\/a><br \/>\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c2\u56de\u3000\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\uff5e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_3\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c3\u56de\u30003\u3064\u306eCRIPSR\/Cas9\u5b9f\u9a13\u7cfb\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_4\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c4\u56de\u3000\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_5\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c5\u56de\u3000\u5909\u7570\u306e\u78ba\u8a8d\u65b9\u6cd5\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_6\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c6\u56de\u3000\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_7\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c7\u56de\u3000\u30ce\u30c3\u30af\u30a4\u30f3\uff5e<\/a><\/p>\n<p>\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u57fa\u790e\u7684\u306a\u5185\u5bb9\uff1e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/crispr-cas9_basic_bid_ts_1\/?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u539f\u7406\u3068\u624b\u5f15\u304d<\/a><\/p>\n<h3>CRISPR\/Cas9\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u59cb\u3081\u308b\u65b9\u306b\u304a\u3059\u3059\u3081\uff01\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3068\u8a66\u85ac\u304c\u30bb\u30c3\u30c8\u306b\u306a\u3063\u305f\u30b9\u30bf\u30fc\u30bf\u30fc\u30d1\u30c3\u30af<\/h3>\n<p>\u6280\u8853\u9769\u65b0\u304c\u8457\u3057\u3044CRISPER\/Cas\u30b2\u30ce\u30e0\u7de8\u96c6\u5206\u91ce\u306b\u304a\u3044\u3066\u3001\u8a66\u85ac\u306e\u9078\u5b9a\u306b\u6238\u60d1\u3046\u65b9\u3082\u5c11\u306a\u304f\u3042\u308a\u307e\u305b\u3093\u3002\u307e\u305fgRNA\uff08\u30ac\u30a4\u30c9RNA\uff09\u306e\u30c7\u30b6\u30a4\u30f3\u65b9\u6cd5\u3084\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u30ce\u30a6\u30cf\u30a6\u306f\u5b9f\u9a13\u7d50\u679c\u3092\u5de6\u53f3\u3059\u308b\u91cd\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u3067\u3059\u3002<br \/>\n\u305d\u3053\u3067\u8b1b\u7fa9\u3001\u5b9f\u6280\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u3001\u5fc5\u8981\u306a\u8a66\u85ac\u3092\u30bb\u30c3\u30c8\u306b\u3057\u305f\u3068\u3066\u3082\u304a\u5f97\u306a\u30d1\u30c3\u30b1\u30fc\u30b8\u3092\u6e96\u5099\u3057\u307e\u3057\u305f\u3002\u8a66\u85ac\u306e\u9078\u5b9a\u304b\u3089\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c8\u307e\u3067\u3001\u30a8\u30ad\u30b9\u30d1\u30fc\u30c8\u304c\u3042\u306a\u305f\u306e\u5b9f\u9a13\u7cfb\u306e\u7acb\u3061\u4e0a\u3052\u3092\u5168\u529b\u3067\u30b5\u30dd\u30fc\u30c8\u3057\u307e\u3059\u3002<\/p>\n<p><b><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/products-and-services\/services\/technical-services\/starter-pack\/genome-editing.html?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u8a73\u7d30\u3092\u898b\u308b<\/a><\/b><\/p>\n<h3>\uff3b\u7121\u6599\u516c\u958b\u4e2d\uff3dCRISPR\u30b2\u30ce\u30e0\u7de8\u96c6\u30ea\u30bd\u30fc\u30b9\u30ac\u30a4\u30c9<\/h3>\n<p>\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u57fa\u790e\u306e\u5fa9\u7fd2\u3001\u3088\u308a\u6df1\u3044\u77e5\u8b58\u306e\u7372\u5f97\u3001\u307e\u305f\u306f\u6700\u65b0\u306e\u7814\u7a76\u30c4\u30fc\u30eb\u306e\u691c\u7d22\u304c\u3067\u304d\u307e\u3059\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u4e2d\u6838\u3068\u3057\u3066\u3001\u521d\u5fc3\u8005\u304b\u3089\u7d4c\u9a13\u8005\u307e\u3067\u3001\u8c4a\u5bcc\u3067\u4fe1\u983c\u3067\u304d\u308b\u6280\u8853\u7684\u306a\u5185\u5bb9\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/genome-editing-resource-guide-form-download.html?CID=bid_clb_gme_r04_jp_cp1497_pjt8452_bid00000_0so_blg_op_awa_oc_s00_ge2_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<p><strong>\u53c2\u8003\u6587\u732e<\/strong><br \/>\n1.Enhanced CRISPR\/Cas-mediated precise genome editing by improved design and delivery of gRNA, Cas9 nuclease, and donor DNA. J.Biotechnology. 2017;241:136-46<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u306f\u3058\u3081\u306b \u524d\u56de\u306e\u30d6\u30ed\u30b0\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u539f\u7406\u3092\u5b66\u3093\u3060A\u3055\u3093\u306f\u3001\u6b21\u306b\u5148\u8f29\u304b\u3089\u30ac\u30a4\u30c9RNA\u30c7\u30b6\u30a4\u30f3\u306b\u304a\u3051\u308b\u6ce8\u610f\u70b9\u3084\u65b9\u6cd5\u3092\u5b66\u3076\u3053\u3068\u306b\u306a\u308a\u307e\u3057\u305f\u3002 \u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\u65b9\u6cd5 \u307e\u305a\u3001\u30ac\u30a4\u30c9RNA\u306e\u3046\u3061\u3001\u6a19\u8b58\u907a\u4f1d\u5b50\u306b\u5bfe\u3057\u3066\u30c7\u30b6\u30a4\u30f3\u304c\u5fc5<\/p>\n","protected":false},"author":975,"featured_media":10134,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[190,175],"division":[],"class_list":{"0":"post-11335","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-crispr-cas9","9":"tag-genome-editing","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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