{"id":11347,"date":"2022-04-06T10:25:05","date_gmt":"2022-04-06T01:25:05","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=11347"},"modified":"2025-03-10T11:32:24","modified_gmt":"2025-03-10T02:32:24","slug":"primer_design_ce_gsd_ts_1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/primer_design_ce_gsd_ts_1\/","title":{"rendered":"PCR\u3084\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\u3069\u3046\u3057\u3066\u3044\u307e\u3059\u304b\uff1f\u2013 Seq It Out\uff035"},"content":{"rendered":"<p>PCR\u3084\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u306f\u3001\u3088\u308a\u826f\u3044\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u5897\u5e45\u3084\u305d\u306e\u5f8c\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u6210\u529f\u306b\u6b20\u304b\u305b\u307e\u305b\u3093\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u6642\u306b\u6ce8\u610f\u3059\u3079\u304d\u3053\u3068\u3060\u3051\u3067\u306a\u304f\u3001\u3059\u3067\u306b\u30c7\u30b6\u30a4\u30f3\u6e08\u307f\u306e\u6700\u9069\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u3042\u3063\u305f\u3089\u3044\u3044\u306a\u3001\u3068\u601d\u3063\u305f\u3053\u3068\u306f\u3042\u308a\u307e\u305b\u3093\u304b\uff1f<\/p>\n<p>\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3059\u308b\u969b\u306e\u30dd\u30a4\u30f3\u30c8\u3084\u4fbf\u5229\u306a\u30c4\u30fc\u30eb\u306b\u3064\u3044\u3066\u8aac\u660e\u3057\u305f\u30d3\u30c7\u30aa\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u30d3\u30c7\u30aa\u306e\u5185\u5bb9\u306b\u3064\u3044\u3066\u4e0b\u8a18\u306b\u7c21\u5358\u306b\u307e\u3068\u3081\u3066\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u3053\u3061\u3089\u3082\u305c\u3072\u3054\u53c2\u8003\u306b\u306a\u3055\u3063\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u969b\u306e\u30dd\u30a4\u30f3\u30c8\u304c\u77e5\u308a\u305f\u3044<br \/>\n\u30fb\u4fbf\u5229\u306a\u30c7\u30b6\u30a4\u30f3\u30c4\u30fc\u30eb\u3092\u63a2\u3057\u3066\u3044\u308b<\/p>\n<p><iframe loading=\"lazy\" title=\"Designing PCR and Sanger Sequencing Primers\u2013 Seq It Out #5\" width=\"760\" height=\"428\" src=\"https:\/\/www.youtube.com\/embed\/P6Nm9Vxyzj0?feature=oembed\" frameborder=\"0\" allow=\"accelerometer; autoplay; clipboard-write; encrypted-media; gyroscope; picture-in-picture; web-share\" referrerpolicy=\"strict-origin-when-cross-origin\" allowfullscreen><\/iframe><\/p>\n<h2>\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306e\u30dd\u30a4\u30f3\u30c8<\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/sanger-sequencing-workflow.html?CID=gsd_cap_sbu_r04_jp_cp1487_pjt8684_gsd00000_0so_blg_op_awa_oc_s00_dne20_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/a>\u3067\u306f\u3001\u30b2\u30ce\u30e0DNA\u3092\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u7528\u3044\u308b\u5834\u5408\u306fPCR\u306b\u3088\u3063\u3066\u30bf\u30fc\u30b2\u30c3\u30c8\u9818\u57df\u3092\u5897\u5e45\u3057\u3001\u6b21\u306b\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u5b9f\u884c\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u7cbe\u88fd\u3055\u308c\u305f\u30d7\u30e9\u30b9\u30df\u30c9DNA\u3092\u7528\u3044\u308b\u5834\u5408\u306f\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u5b9f\u884c\u3059\u308b\u3060\u3051\u3067\u6e08\u307f\u307e\u3059\u3002<\/p>\n<p>PCR\u53cd\u5fdc\u306b\u306f\u3001\u5897\u5e45\u3059\u308b\u914d\u5217\u306e\u9818\u57df\u3092\u6c7a\u5b9a\u3059\u308b\u9806\u65b9\u5411\u3068\u9006\u65b9\u5411\u306e2\u3064\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u5fc5\u8981\u3067\u3059\u304c\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3067\u306f1\u672c\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u307f\u304c\u53cd\u5fdc\u306b\u4f7f\u7528\u3055\u308c\u308b\u3068\u3044\u3046\u70b9\u3067PCR\u53cd\u5fdc\u3068\u306f\u7570\u306a\u308a\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u901a\u5e38\u3001\u7279\u5b9a\u306ePCR\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u306b\u5bfe\u3057\u3066\u30012\u3064\u306e\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u304c\u8a2d\u5b9a\u3055\u308c\u307e\u3059\u30021\u3064\u306f\u9806\u65b9\u5411\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u3001\u3082\u30461\u3064\u306f\u9006\u65b9\u5411\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u3067\u3059\u3002<\/p>\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u306f\u3001\u57fa\u672c\u7684\u306aPCR\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u3060\u3051\u3067\u306a\u304f\u3001\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3084\u3001\u5b9a\u91cf\u5206\u6790\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u5b9f\u9a13\u7cfb\u306b\u95a2\u308f\u308b\u91cd\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u3067\u3059\u3002<\/p>\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3059\u308b\u969b\u306b\u8003\u616e\u3059\u308b\u30dd\u30a4\u30f3\u30c8\u304c\u3044\u304f\u3064\u304b\u3042\u308a\u307e\u3059\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u9577\u3055\u306f18\u301c22\u5869\u57fa\u7a0b\u5ea6\u3067\u30c7\u30b6\u30a4\u30f3\u3057\u307e\u3059\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306eGC\u542b\u91cf\u306f50\uff05\u304b\u308955\uff05\u306b\u306a\u308b\u3088\u3046\u306b\u3057\u307e\u3059\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306e3 \u2018\u672b\u7aef\u306e\u6700\u5f8c\u306e1\u5869\u57fa\u3082\u3057\u304f\u306f2\u5869\u57fa\u3092G\u304bC\u306b\u3057\u307e\u3059\uff08GC\u30ed\u30c3\u30af\uff09\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u878d\u89e3\u6e29\u5ea6\u306f\u300150\u2103\u304b\u308955\u2103\u4f4d\u306b\u306a\u308b\u3088\u3046\u306b\u3057\u307e\u3059\u3002<br \/>\n\u30fb\u540c\u4e00\u5869\u57fa\u304c\u9023\u7d9a\u3059\u308b\u9818\u57df\u3092\u907f\u3051\u3066\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u307e\u3059\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u5185\u306b\u30824\u3064\u4ee5\u4e0a\u306e\u9023\u7d9a\u5869\u57fa\u306f\u907f\u3051\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u7121\u6599\u306ePCR\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u691c\u7d22\u30c4\u30fc\u30eb\u306e\u3054\u7d39\u4ecb<\/h2>\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u8a2d\u8a08\u306f\u7c21\u5358\u3067\u306f\u306a\u3044\u305f\u3081\u3001\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u3088\u3063\u3066\u306f\u3001\u5b9f\u9a13\u306b\u4f59\u8a08\u306a\u6642\u9593\u3084\u30b3\u30b9\u30c8\u304c\u304b\u304b\u3063\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u3082\u3063\u3068\u7c21\u5358\u306a\u65b9\u6cd5\u3092\u304a\u63a2\u3057\u306e\u65b9\u3078\u3001\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u306e\u691c\u7d22\u30c4\u30fc\u30eb\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/pre-designed-primers-pcr-sanger-sequencing.html?CID=gsd_cap_sbu_r04_jp_cp1487_pjt8684_gsd00000_0so_blg_op_awa_oc_s00_dne20_Social_LAB\" target=\"_blank\" rel=\"noopener\">Primer Designer\u30c4\u30fc\u30eb<\/a>\u306f\u3001\u7121\u6599\u3067PCR\u7528\u307e\u305f\u306f\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u691c\u7d22\u3067\u304d\u308b\u30c4\u30fc\u30eb\u3067\u3042\u308a\u3001\u30d2\u30c8\u30a8\u30af\u30bd\u30fc\u30e0\u3068\u30d2\u30c8\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u30b2\u30ce\u30e0\u3092\u30ab\u30d0\u30fc\u3059\u308b600,000\u3092\u8d85\u3048\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u30da\u30a2\u304c\u542b\u307e\u308c\u3066\u3044\u307e\u3059\u3002\u30b5\u30f3\u30d7\u30eb\u306e\u30a2\u30f3\u30d7\u30ea\u30b3\u30f3\u306e\u9577\u3055\u3068\u5bfe\u8c61\u907a\u4f1d\u5b50\u540d\u306a\u3069\u3092\u9078\u629e\u3057\u3066\u3001\u691c\u7d22\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\u88c5\u7f6e\u3067\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\uff08NGS\uff09\u306e\u30c7\u30fc\u30bf\u78ba\u8a8d\u3092\u884c\u3046\u5834\u5408\u3001\u3053\u306e\u30c4\u30fc\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3068\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3092\u5927\u5e45\u306b\u7c21\u7d20\u5316\u3067\u304d\u307e\u3059\u3002\u3053\u308c\u306f\u3001NGS\u3067\u5f97\u3089\u308c\u305f.vcf\u30d5\u30a1\u30a4\u30eb\u3092\u30a2\u30c3\u30d7\u30ed\u30fc\u30c9\u3059\u308b\u3053\u3068\u3067\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>Ion Torrent\u30b7\u30fc\u30b1\u30f3\u30b5\u30fc\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u30a4\u30aa\u30f3\u30ec\u30dd\u30fc\u30bf\u30fc\u304b\u3089Primer Designer\u30c4\u30fc\u30eb\u306b.vcf\u30d5\u30a1\u30a4\u30eb\u3092\u30a2\u30c3\u30d7\u30ed\u30fc\u30c9\u3067\u304d\u308b\u305f\u3081\u3055\u3089\u306b\u4fbf\u5229\u306b\u306a\u308a\u3001\u6570\u56de\u30af\u30ea\u30c3\u30af\u3059\u308b\u3060\u3051\u3067\u9069\u5207\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u898b\u3064\u3051\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30fb\u30ab\u30b9\u30bf\u30e0\u3067\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3059\u308b\u5834\u5408\u3001\u591a\u304f\u306e\u9805\u76ee\u3092\u691c\u8a0e\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\n\u30fb\u7121\u6599\u3067\u4f7f\u3048\u308b<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/pre-designed-primers-pcr-sanger-sequencing.html?CID=gsd_cap_sbu_r04_jp_cp1487_pjt8684_gsd00000_0so_blg_op_awa_oc_s00_dne20_Social_LAB\" target=\"_blank\" rel=\"noopener\">Primer Designer\u30c4\u30fc\u30eb<\/a>\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u3067\u304a\u56f0\u308a\u306e\u65b9\u306f\u3001\u305c\u3072<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/pre-designed-primers-pcr-sanger-sequencing.html?CID=gsd_cap_sbu_r04_jp_cp1487_pjt8684_gsd00000_0so_blg_op_awa_oc_s00_dne20_Social_LAB\" target=\"_blank\" rel=\"noopener\">Primer Designer\u30c4\u30fc\u30eb<\/a>\u3092\u691c\u8a0e\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044<\/p>\n<h3>\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b7\u30f3\u30b0\u3000\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/sanger-sequencing-guide-download.html?CID=gsd_cap_sbu_r04_jp_cp1487_pjt8684_gsd00000_0so_blg_op_awa_oc_s00_dne20_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>PCR\u3084\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u306f\u3001\u3088\u308a\u826f\u3044\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u5897\u5e45\u3084\u305d\u306e\u5f8c\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u6210\u529f\u306b\u6b20\u304b\u305b\u307e\u305b\u3093\u3002\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u6642\u306b\u6ce8\u610f\u3059\u3079\u304d\u3053\u3068\u3060\u3051\u3067\u306a\u304f\u3001\u3059\u3067\u306b\u30c7\u30b6\u30a4\u30f3\u6e08\u307f\u306e\u6700\u9069\u306a\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u3042\u3063\u305f\u3089\u3044\u3044\u306a\u3001<\/p>\n","protected":false},"author":975,"featured_media":9657,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[155],"tags":[9,183,10],"division":[],"class_list":{"0":"post-11347","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-else","8":"tag-pcr","9":"tag-sanger-seq","10":"tag-primer","11":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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