{"id":11349,"date":"2022-04-11T11:03:05","date_gmt":"2022-04-11T02:03:05","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=11349"},"modified":"2025-01-23T12:08:15","modified_gmt":"2025-01-23T03:08:15","slug":"imaging_evos_bid_ts_2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/imaging_evos_bid_ts_2\/","title":{"rendered":"\u7b2c2\u56de\u3000\u86cd\u5149\u89b3\u5bdf\u306b\u304a\u3051\u308b\u6f0f\u308c\u8fbc\u307f\u3068\u306f\uff1f\u5177\u4f53\u4f8b\u304b\u3089\u3088\u308a\u8a73\u3057\u304f\uff01"},"content":{"rendered":"

\u524d\u56de<\/a>\u306b\u5f15\u304d\u7d9a\u304d\u4eca\u56de\u306e\u30c6\u30fc\u30de\u3082\u300c\u86cd\u5149\u89b3\u5bdf\u306b\u304a\u3051\u308b\u6f0f\u308c\u8fbc\u307f\u300d\u3067\u3059\u3002\u524d\u56de\u306f\u3001\u86cd\u5149\u89b3\u5bdf\u306b\u304a\u3051\u308b\u6f0f\u308c\u8fbc\u307f\u306b\u3064\u3044\u3066\u6982\u5ff5\u7684\u306a\u8aac\u660e\u3092\u3057\u307e\u3057\u305f\u3002\u4eca\u56de\u306f\u5177\u4f53\u7684\u306a\u4f8b\u3092\u6319\u3052\u3064\u3064\u3001\u3088\u308a\u8a73\u3057\u304f\u7d39\u4ecb\u3057\u3066\u3044\u304d\u307e\u3059\u3002<\/p>\n

\u86cd\u5149\u89b3\u5bdf\u306b\u304a\u3051\u308b\u6f0f\u308c\u8fbc\u307f\u306e\u5b9f\u4f8b<\/h2>\n

Invitrogen\u2122 Alexa Fluor\u2122 594 Phalloidin\u3067\u67d3\u8272\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u5f0a\u793e\u306e\u86cd\u5149\u9855\u5fae\u93e1Invitrogen\u2122 EVOS\u2122 M5000 Imaging System\u3067\u64ae\u5f71\u3057\u305f\u3082\u306e\u3092\u4f8b\u306b\u898b\u3066\u307f\u307e\u3059\u3002
\nInvitrogen\u2122 Alexa Fluor\u2122 594 Dye\u306f\u6700\u5927\u52b1\u8d77\u6ce2\u9577581 nm\u3001\u6700\u5927\u86cd\u5149\u6ce2\u9577609 nm\u306e\u86cd\u5149\u8272\u7d20\u3067\u3001Texas Red\u2122\u30d5\u30a3\u30eb\u30bf\u30fc\u3067\u306e\u89b3\u5bdf\u304c\u9069\u3057\u3066\u3044\u308b\u86cd\u5149\u8272\u7d20\u3068\u306a\u308a\u307e\u3059\u3002
\n\"\"
\n\u3068\u3053\u308d\u304c\u3001Invitrogen\u2122 Alexa Fluor\u2122 647 Dye\u306e\u89b3\u5bdf\u306b\u4f7f\u7528\u3055\u308c\u308bCy5\u00ae\u30d5\u30a3\u30eb\u30bf\u30fc\u30bb\u30c3\u30c8\u3067\u3082Alexa Fluor 594 Dye\u306f\u308f\u305a\u304b\u3067\u3059\u304c\u52b1\u8d77\u3055\u308c\u3001\u86cd\u5149\u3092\u691c\u51fa\u3067\u304d\u3066\u3057\u307e\u3044\u307e\u3059\u3002
\n\"\"
\n\u3053\u308c\u306fAlexa Fluor 594 Dye\u3068Alexa Fluor 647 Dye\u3092\u540c\u6642\u306b\u67d3\u8272\u3059\u308b\u3068Alexa Fluor 647 Dye\u3092\u691c\u51fa\u3059\u308b\u969b\u306bAlexa Fluor 594 Dye\u306e\u86cd\u5149\u3082\u691c\u51fa\u3057\u3066\u3057\u307e\u3063\u3066\u3044\u308b\u53ef\u80fd\u6027\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002
\n\u305d\u308c\u3067\u306f\u3053\u306e\u86cd\u5149\u306e\u6f0f\u308c\u8fbc\u307f\u3092\u9632\u3050\u306b\u306f\u3069\u3046\u3059\u308c\u3070\u826f\u3044\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

\u89e3\u6c7a\u7b561\u3000\u4f7f\u7528\u3059\u308b\u86cd\u5149\u8272\u7d20\u306e\u6ce2\u9577\u3092\u96e2\u3059<\/h3>\n

\u6709\u52b9\u306a\u65b9\u6cd5\u306f\u3001\u4f7f\u7528\u3059\u308b\u86cd\u5149\u8272\u7d20\u540c\u58eb\u306e\u6ce2\u9577\u3092\u96e2\u3059\u65b9\u6cd5\u3067\u3059\u3002\u524d\u56de\u306e\u30d6\u30ed\u30b0\u3067\u3054\u7d39\u4ecb\u3057\u305f\u304a\u3059\u3059\u3081\u306e\u86cd\u5149\u8272\u7d20\u306e\u7d44\u307f\u5408\u308f\u305b\u3067\u3042\u308bInvitrogen\u2122 Alexa Fluor\u2122 488 Dye\u3068Alexa Fluor 594 Dye\u3067\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002Alexa Fluor 488 Dye\u306e\u86cd\u5149\u6ce2\u9577\u30b9\u30da\u30af\u30c8\u30eb\u306f\u3001Alexa Fluor 594 Dye\u306e\u691c\u51fa\u3067\u4f7f\u7528\u3059\u308bTexas Red\u30d5\u30a3\u30eb\u30bf\u30fc\u30bb\u30c3\u30c8\u3067\u306f\u52b1\u8d77\u3055\u308c\u306a\u3044\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u3088\u3063\u3066\u52b1\u8d77\u6ce2\u9577\u306b\u95a2\u3057\u3066\u306f\u308f\u305a\u304b\u306a\u304c\u3089\u691c\u51fa\u53ef\u80fd\u3068\u306a\u3063\u3066\u304a\u308a\u307e\u3059\u304c\u3001\u52b1\u8d77\u81ea\u4f53\u3055\u308c\u306a\u3044\u305f\u3081\u306bAlexa Fluor 594 Dye\u306e\u89b3\u5bdf\u306e\u969b\u306b\u6f0f\u308c\u8fbc\u307e\u306a\u3044\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002
\n\"\"
\nAlexa Fluor 594 Dye\u306e\u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u3082\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002Alexa Fluor 488 Dye\u306e\u691c\u51fa\u3067\u4f7f\u7528\u3059\u308bGFP\u306e\u30d5\u30a3\u30eb\u30bf\u30fc\u30bb\u30c3\u30c8\u3067\u306f\u308f\u305a\u304b\u306b\u52b1\u8d77\u3055\u308c\u3066\u3044\u308b\u3082\u306e\u306e\u3001\u86cd\u5149\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u306a\u3044\u305f\u3081\u306b\u691c\u51fa\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002
\n\"\"
\n\u3053\u306e\u3088\u3046\u306b\u4f7f\u7528\u3059\u308b\u86cd\u5149\u8272\u7d20\u540c\u58eb\u306e\u6ce2\u9577\u3092\u96e2\u3059\u3053\u3068\u3067\u86cd\u5149\u306e\u6f0f\u308c\u8fbc\u307f\u3092\u9632\u3050\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u89e3\u6c7a\u7b562\u3000\u6f0f\u308c\u8fbc\u3080\u53ef\u80fd\u6027\u306e\u3042\u308b\u86cd\u5149\u8272\u7d20\u306f\u86cd\u5149\u5f37\u5ea6\u306e\u5c0f\u3055\u3044\u3082\u306e\u306b\u3059\u308b<\/h3>\n

\u5b9f\u9a13\u306e\u90fd\u5408\u4e0a\u3001\u6f0f\u308c\u8fbc\u307f\u304c\u751f\u3058\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u7d44\u307f\u5408\u308f\u305b\u3092\u4f7f\u7528\u3059\u308b\u5834\u5408\u3001\u6f0f\u308c\u8fbc\u3080\u53ef\u80fd\u6027\u306e\u3042\u308b\u86cd\u5149\u8272\u7d20\u306f\u86cd\u5149\u5f37\u5ea6\u306e\u5c0f\u3055\u3044\u3082\u306e\u306b\u3057\u307e\u3059\u3002
\n2\u3064\u306e\u86cd\u5149\u8272\u7d20\u3092\u691c\u51fa\u3059\u308b\u5834\u5408\u30012\u3064\u306e\u86cd\u5149\u8272\u7d20\u306e\u6ce2\u9577\u304c\u96e2\u308c\u3066\u304a\u308a\u3001\u540c\u7a0b\u5ea6\u306e\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u3092\u793a\u3057\u3066\u3044\u308b\u306e\u3067\u3042\u308c\u3070\u3001\u6f0f\u308c\u8fbc\u307f\u91cf\u306f\u308f\u305a\u304b\u3067\u3059\u306e\u3067\u3001\u86cd\u5149\u306e\u6f0f\u308c\u8fbc\u307f\u304c\u6c17\u306b\u306a\u308b\u3053\u3068\u306f\u5c11\u306a\u3044\u3067\u3059\u3002
\n\u4e00\u65b9\u3067\u767a\u73fe\u91cf\u3084\u6297\u4f53\u306e\u6027\u80fd\u304c\u7570\u306a\u308b\u306a\u3069\u306e\u7406\u7531\u306b\u3088\u308a\u3001\u86cd\u5149\u30b7\u30b0\u30ca\u30eb\u306e\u5dee\u304c\u5927\u304d\u3044\u5834\u5408\u3001\u5404\u86cd\u5149\u306e\u691c\u51fa\u306e\u969b\u306e\u9732\u5149\u6642\u9593\u304c\u5909\u308f\u308a\u3001\u6f0f\u308c\u8fbc\u307f\u304c\u751f\u3058\u308b\u30ea\u30b9\u30af\u304c\u9ad8\u307e\u308a\u307e\u3059\u3002\u6838\u67d3\u8272\u8a66\u85ac\u3001\u7d30\u80de\u306e\u30c8\u30ec\u30fc\u30b7\u30f3\u30b0\u306a\u3069\u306b\u4f7f\u7528\u3055\u308c\u308bCFSE\u3001F-\u30a2\u30af\u30c1\u30f3\u306e\u67d3\u8272\u306b\u4f7f\u7528\u3055\u308c\u308bPhalloidin\u3001\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u306e\u67d3\u8272\u306b\u4f7f\u7528\u3055\u308c\u308bInvitrogen\u2122 MitoTracker\u2122 Dye\u306a\u3069\u306f\u6297\u4f53\u3088\u308a\u3082\u5f37\u3044\u30b7\u30b0\u30ca\u30eb\u3092\u793a\u3059\u306e\u3067\u540c\u6642\u306b\u4f7f\u7528\u3059\u308b\u969b\u306b\u306f\u7279\u306b\u6ce8\u610f\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u86cd\u5149\u306e\u6f0f\u308c\u8fbc\u307f\u304c\u751f\u3058\u3066\u3057\u307e\u3063\u305f\u5834\u5408\u3001\u672c\u6765\u86cd\u5149\u304c\u767a\u751f\u3057\u3066\u3044\u306a\u3044\u9818\u57df\u3067\u86cd\u5149\u304c\u89b3\u5bdf\u3055\u308c\u3066\u3057\u307e\u3044\u3001\u30c7\u30fc\u30bf\u306e\u89e3\u91c8\u3092\u9593\u9055\u3063\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002
\n\u591a\u91cd\u67d3\u8272\u3092\u884c\u3046\u5834\u5408\u306b\u306f\u3001\u86cd\u5149\u8272\u7d20\u306e\u6ce2\u9577\u3001\u86cd\u5149\u5f37\u5ea6\u3092\u610f\u8b58\u3057\u3066\u3001\u4f7f\u7528\u3059\u308b\u86cd\u5149\u8272\u7d20\u3092\u9078\u629e\u3057\u307e\u3057\u3087\u3046\u3002
\n\u5408\u308f\u305b\u3066\u3001\uff11\u8272\u306e\u307f\u3067\u67d3\u8272\u3092\u884c\u3063\u305f\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30b5\u30f3\u30d7\u30eb\u3092\u4f5c\u6210\u3059\u308b\u3053\u3068\u3067\u6f0f\u308c\u8fbc\u307f\u304c\u751f\u3058\u3066\u3044\u308b\u304b\u5224\u65ad\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u306e\u3067\u3054\u53c2\u8003\u306b\u306a\u3055\u3063\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u3054\u81ea\u8eab\u3067\u86cd\u5149\u8272\u7d20\u3092\u9078\u629e\u3055\u308c\u308b\u969b\u306b\u306f\u3001\u5f53\u793e\u304c\u63d0\u4f9b\u3059\u308b\u30a6\u30a7\u30d6\u30a2\u30d7\u30ea\u306b\u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u30d3\u30e5\u30fc\u30a2\u30fc\u304c\u3042\u308a\u307e\u3059\u3002
\n\u6ce2\u5f62\u30c7\u30fc\u30bf\u3092\u78ba\u8a8d\u3057\u305f\u3044\u86cd\u5149\u8272\u7d20\u3092\u9078\u629e\u3059\u308b\u3068\u3001\u52b1\u8d77\u6ce2\u9577\u3068\u86cd\u5149\u6ce2\u9577\u304c\u30b0\u30e9\u30d5\u8868\u793a\u3055\u308c\u3001\u52b1\u8d77\u86cd\u5149\u30b9\u30da\u30af\u30c8\u30eb\u3068\u5404\u7a2e\u30d5\u30a3\u30eb\u30bf\u30fc\u306e\u9069\u5408\u6027\u3092\u7c21\u5358\u306b\u6bd4\u8f03\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u305c\u3072\u3054\u5229\u7528\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u3010\u7121\u6599\u516c\u958b\u4e2d\u3011Molecular Probes\u86cd\u5149\u6559\u5ba4PDF\u7248<\/h2>\n

Invitrogen\u2122 Molecular Probes\u2122 \u86cd\u5149\u6559\u5ba4\u3067\u306f\u3001\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea \uff08FP\uff09 \u3092\u5229\u7528\u3057\u305f\u7d30\u80de\u89b3\u5bdf\u3084 FP \u878d\u5408\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30c7\u30b6\u30a4\u30f3\u3001\u307e\u305f\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u7d30\u80de\u306b\u5c0e\u5165\u3059\u308b\u305f\u3081\u306e\u30b3\u30c4\u3092\u3054\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u524d\u56de\u306b\u5f15\u304d\u7d9a\u304d\u4eca\u56de\u306e\u30c6\u30fc\u30de\u3082\u300c\u86cd\u5149\u89b3\u5bdf\u306b\u304a\u3051\u308b\u6f0f\u308c\u8fbc\u307f\u300d\u3067\u3059\u3002\u524d\u56de\u306f\u3001\u86cd\u5149\u89b3\u5bdf\u306b\u304a\u3051\u308b\u6f0f\u308c\u8fbc\u307f\u306b\u3064\u3044\u3066\u6982\u5ff5\u7684\u306a\u8aac\u660e\u3092\u3057\u307e\u3057\u305f\u3002\u4eca\u56de\u306f\u5177\u4f53\u7684\u306a\u4f8b\u3092\u6319\u3052\u3064\u3064\u3001\u3088\u308a\u8a73\u3057\u304f\u7d39\u4ecb\u3057\u3066\u3044\u304d\u307e\u3059\u3002 \u86cd\u5149\u89b3\u5bdf\u306b\u304a\u3051\u308b\u6f0f\u308c\u8fbc\u307f\u306e\u5b9f\u4f8b 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