{"id":114,"date":"2015-10-15T00:00:00","date_gmt":"2015-10-15T00:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ecoli-genotype\/"},"modified":"2024-08-27T11:40:28","modified_gmt":"2024-08-27T02:40:28","slug":"ecoli-genotype","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ecoli-genotype\/","title":{"rendered":"endA\u3063\u3066\u3069\u3046\u3044\u3046\u610f\u5473\uff1f\u77e5\u3063\u3066\u304a\u304f\u3068\u5f79\u7acb\u3064\u4e3b\u306a\u5927\u8178\u83cc\u306e\u907a\u4f1d\u5b50\u578b\u307e\u3068\u3081"},"content":{"rendered":"

\u88fd\u54c1\u30ab\u30bf\u30ed\u30b0\u3067\u30b3\u30f3\u30d4\u30c6\u30f3\u30c8\u30bb\u30eb\u3092\u63a2\u3057\u3066\u3044\u308b\u3068\u3001\u6b20\u640d\u3057\u3066\u3044\u308b\u907a\u4f1d\u5b50\u304c\u8868\u8a18\u3055\u308c\u3066\u3044\u307e\u3059\u304c\u3001\u30d1\u30c3\u3068\u898b\u3067\u3069\u3093\u306a\u6a5f\u80fd\u304c\u6b20\u640d\u3057\u3066\u3044\u308b\u306e\u304b\u3088\u304f\u5206\u304b\u308a\u307e\u305b\u3093\u3088\u306d\uff1f<\/p>\n

\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u3088\u304f\u4f7f\u7528\u3055\u308c\u308b\u5927\u8178\u83cc\u306e\u907a\u4f1d\u5b50\u578b\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u306e\u3067\u3054\u7d39\u4ecb\u3057\u307e\u3059\uff01<\/p>\n

\u5927\u8178\u83cc\u30db\u30b9\u30c8\u306e\u907a\u4f1d\u5b50\u578b<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u5927\u8178\u83cc\u540d<\/th>\n<\/th>\n\u907a\u4f1d\u5b50\u578b<\/th>\n<\/tr>\n
BL21-AI<\/a><\/td>\n<\/td>\nF\u2013<\/sup> omp<\/i>T hsd<\/i>SB(rB<\/sub>\u2013<\/sup>, mB<\/sub>\u2013<\/sup>) gal dcm ara<\/i>B::T7RNAP-tetA<\/i><\/td>\n<\/tr>\n
BL21(DE3)<\/a><\/td>\n<\/td>\nF\u2013<\/sup> omp<\/i>T hsd<\/i>SB(rB<\/sub>\u2013<\/sup>, mB<\/sub>\u2013<\/sup>) gal dcm <\/i>(DE3)<\/td>\n<\/tr>\n
BL21(DE3)pLysS<\/a><\/td>\n<\/td>\nF\u2013<\/sup> omp<\/i>T hsd<\/i>SB(rB<\/sub>\u2013<\/sup>, mB<\/sub>\u2013<\/sup>) gal dcm <\/i>(DE3) pLysS (CamR<\/sup>)<\/td>\n<\/tr>\n
BL21(DE3)pLysE<\/a><\/td>\n<\/td>\nF\u2013<\/sup> omp<\/i>T hsd<\/i>SB(rB<\/sub>\u2013<\/sup>, mB<\/sub>\u2013<\/sup>) gal dcm <\/i>(DE3) pLysE (CamR<\/sup>)<\/td>\n<\/tr>\n
BL21 Star\u2122(DE3)<\/a><\/td>\n<\/td>\nF\u2013<\/sup> omp<\/i>T hsd<\/i>SB(rB<\/sub>\u2013<\/sup>, mB<\/sub>\u2013<\/sup>) gal dcm rne<\/i>131 (DE3)<\/td>\n<\/tr>\n
BL21 Star\u2122 (DE3)pLysS<\/a><\/td>\n<\/td>\nF\u2013<\/sup> omp<\/i>T hsd<\/i>SB(rB<\/sub>\u2013<\/sup>, mB<\/sub>\u2013<\/sup>) gal dcm rne<\/i>131 (DE3) pLysS (CamR<\/sup>)<\/td>\n<\/tr>\n
ccd<\/i>B Survival\u21222<\/a><\/td>\n<\/td>\nF- mcr<\/i>A \u0394(mrr<\/i>–hsd<\/i>RMS-mcr<\/i>BC) \u03a680lac<\/i>Z\u0394M15 \u0394lac<\/i>X74 rec<\/i>A1 ara<\/i>\u0394139 \u0394(ara<\/i>–leu<\/i>)7697 gal<\/i>U gal<\/i>K rps<\/i>L (StrR<\/sup>) end<\/i>A1 nup<\/i>G fhuA::IS2<\/i><\/td>\n<\/tr>\n
DB3.1\u2122<\/td>\n<\/td>\nF\u2013<\/sup> gyr<\/i>A462 end<\/i>A1 \u0394(sr1-rec<\/i>A) mcr<\/i>B mrr hsd<\/i>S20(rB<\/sub>\u2013<\/sup>, mB<\/sub>\u2013<\/sup>) sup<\/i>E44 ara<\/i>-14 gal<\/i>K2 lac<\/i>Y1 pro<\/i>A2 rps<\/i>L20(SmR<\/sup>) xyl<\/i>-5 \u03bb\u2013 leu mtl<\/i>1<\/td>\n<\/tr>\n
DH5\u03b1\u2122<\/a><\/td>\n<\/td>\nF\u2013<\/sup> \u03a680lac<\/i>Z\u0394M15 \u0394(lac<\/i>ZYA-arg<\/i>F) U169 rec<\/i>A1 end<\/i>A1 hsd<\/i>R17 (rK<\/sub>\u2013<\/sup>, mK<\/sub>+<\/sup>) pho<\/i>A sup<\/i>E44 \u03bb\u2013 thi<\/i>-1 gyr<\/i>A96 rel<\/i>A1<\/td>\n<\/tr>\n
DH5\u03b1\u2122 T1R<\/sup><\/a><\/td>\n<\/td>\nF\u2013<\/sup> \u03a680lac<\/i>Z\u0394M15 \u0394(lac<\/i>ZYA-arg<\/i>F)U169 rec<\/i>A1 end<\/i>A1 hsd<\/i>R17 (rK<\/sub>\u2013<\/sup>, mK<\/sub>+<\/sup>) pho<\/i>A sup<\/i>E44 thi<\/i>-1 gyr<\/i>A96 rel<\/i>A1 ton<\/i>A<\/td>\n<\/tr>\n
DH10B\u2122<\/a><\/td>\n<\/td>\nF\u2013<\/sup> mcr<\/i>A \u0394(mrr<\/i>–hsd<\/i>RMS-mcr<\/i>BC) \u03a680lac<\/i>Z\u0394M15 \u0394lac<\/i>X74 rec<\/i>A1 end<\/i>A1 ara<\/i>D139 \u0394(ara leu<\/i>) 7697 gal<\/i>U gal<\/i>K rps<\/i>L nup<\/i>G \u03bb\u2013<\/td>\n<\/tr>\n
DH10B\u2122 T1R<\/sup><\/a><\/td>\n<\/td>\nF\u2013<\/sup> mcr<\/i>A \u0394(mrr<\/i>–hsd<\/i>RMS-mcr<\/i>BC) \u03a680lac<\/i>Z\u0394M15 \u0394lac<\/i>X74 rec<\/i>A1 end<\/i>A1 ara<\/i>D139 \u0394(ara leu<\/i>) 7697 gal<\/i>U gal<\/i>K rps<\/i>L nup<\/i>G \u03bb\u2013 ton<\/i>A<\/td>\n<\/tr>\n
DH10Bac\u2122<\/a><\/td>\n<\/td>\nF\u2013<\/sup> mcr<\/i>A \u0394(mrr<\/i>–hsd<\/i>RMS-mcr<\/i>BC) \u03a680lac<\/i>Z\u0394M15 \u0394lac<\/i>X74 rec<\/i>A1 end<\/i>A1 ara<\/i>D139 \u0394(ara leu<\/i>) 7697 gal<\/i>U gal<\/i>K \u03bb\u2013 rps<\/i>L nup<\/i>G\/ pMON14272\/pMON7124<\/td>\n<\/tr>\n
DH12S\u2122<\/a><\/td>\n<\/td>\n\u03a680d lac<\/i>Z \u0394M15 mcrA<\/i> \u0394(mrr-hsd<\/i>RMS-mcrBC) ara<\/i>D139 \u0394(ara, leu<\/i>)7697 \u0394lac<\/i>X74 gal<\/i>U gal<\/i>K rps<\/i>L(StrR<\/sup>) nup<\/i>G rec<\/i>A1 \/ F\u2019 {pro<\/i>AB+<\/sup> lac<\/i>Iq<\/sup> lac<\/i>ZDM15}<\/td>\n<\/tr>\n
INV110<\/a><\/td>\n<\/td>\nF\u00b4 {tra<\/i>\u039436 pro<\/i>AB lac<\/i>Iq lac<\/i>Z\u0394M15} rps<\/i>L (StrR<\/sup>) thr leu end<\/i>A thi<\/i>-1 lac<\/i>Y gal<\/i>K gal<\/i>T ara ton<\/i>A tsx dam dcm sup<\/i>E44 \u0394(lac<\/i>–pro<\/i>AB) \u0394(mcr<\/i>C-mrr<\/i>)102::Tn10 (TetR<\/sup>)<\/td>\n<\/tr>\n
INV\u03b1F\u00b4<\/a><\/td>\n<\/td>\nF\u00b4 end<\/i>A1 rec<\/i>A1 hsd<\/i>R17 (rK<\/sub>\u2013<\/sup>, mK<\/sub>+<\/sup>) sup<\/i>E44 thi<\/i>-1 gyr<\/i>A96 rel<\/i>A1 \u03a680lac<\/i>Z \u0394M15 \u0394lac<\/i>ZYA-arg<\/i>F)U169<\/i><\/td>\n<\/tr>\n
Mach1\u2122 T1R<\/sup><\/a><\/td>\n<\/td>\nF\u2013<\/sup> \u03a680lac<\/i>Z\u0394M15 \u0394lac<\/i>X74 hsd<\/i>R(rK<\/sub>\u2013<\/sup>, mK<\/sub>+<\/sup>) \u0394rec<\/i>A1398 end<\/i>A1 ton<\/i>A<\/td>\n<\/tr>\n
MegaX DH10B\u2122 T1R<\/sup><\/a><\/td>\n<\/td>\nF\u2013<\/sup> mcr<\/i>A \u0394(mrr<\/i>–hsd<\/i>RMS-mcr<\/i>BC) \u03a680lac<\/i>Z\u0394M15 \u0394lac<\/i>X74 rec<\/i>A1 end<\/i>A1 ara<\/i>D139 \u0394(ara leu<\/i>)7697 gal<\/i>U gal<\/i>K \u03bb\u2013 rps<\/i>L nup<\/i>G ton<\/i>A<\/td>\n<\/tr>\n
OmniMAX\u2122 2 T1R<\/sup><\/a><\/td>\n<\/td>\nF\u00b4 {pro<\/i>AB+ lac<\/i>Iq lac<\/i>Z\u0394M15 Tn10(TetR<\/sup>) \u0394(ccd<\/i>AB)} mcr<\/i>A \u0394(mrr<\/i>–hsd<\/i>RMS-mcr<\/i>BC) \u03a680lac<\/i>Z\u0394M15 \u0394(lac<\/i>ZYA-arg<\/i>F) U169 end<\/i>A1 rec<\/i>A1 sup<\/i>E44 thi<\/i>-1 gyr<\/i>A96 rel<\/i>A1 ton<\/i>A pan<\/i>D<\/td>\n<\/tr>\n
PIR1<\/a><\/td>\n<\/td>\nF\u2013<\/sup> \u2206lac<\/i>169 rpo<\/i>S(am) rob<\/i>A1 cre<\/i>C510 hsd<\/i>R514 end<\/i>A rec<\/i>A1 uidA(\u2206Mlu I)::pir-116<\/i><\/td>\n<\/tr>\n
PIR2<\/a><\/td>\n<\/td>\nF\u2013<\/sup> \u2206lac<\/i>169 rpo<\/i>S(am) rob<\/i>A1 cre<\/i>C510 hsd<\/i>R514 end<\/i>A rec<\/i>A1 uidA(\u2206Mlu I)::pir<\/i><\/td>\n<\/tr>\n
Stbl2\u2122<\/a><\/td>\n<\/td>\nF\u2013<\/sup> mcr<\/i>A \u0394(mcr<\/i>BC-hsd<\/i>RMS-mrr<\/i>) rec<\/i>A1 end<\/i>A1 lon gyr<\/i>A96 thi sup<\/i>E44 rel<\/i>A1 \u03bb\u2013 \u0394(lac<\/i>–pro<\/i>AB)<\/td>\n<\/tr>\n
Stbl3\u2122<\/a><\/td>\n<\/td>\nF\u2013<\/sup> mcr<\/i>B mrr hsd<\/i>S20 (rB<\/sub>\u2013<\/sup>, mB<\/sub>\u2013<\/sup>) rec<\/i>A13 sup<\/i>E44 ara<\/i>-14 gal<\/i>K2 lac<\/i>Y1 pro<\/i>A2 rps<\/i>L20 (StrR ) xyl<\/i>-5 \u03bb\u2013 leu mtl<\/i>-1<\/td>\n<\/tr>\n
Stbl4\u2122<\/a><\/td>\n<\/td>\nmcr<\/i>A \u0394(mcr<\/i>BC-hsd<\/i>RMS-mrr<\/i>) rec<\/i>A1 end<\/i>A1 gyr<\/i>A96 gal<\/i>– thi<\/i>-1 sup<\/i>E44 \u03bb\u2013 rel<\/i>A1 \u0394(lac<\/i>–pro<\/i>AB)\/F\u00b4 pro<\/i>AB+ lac<\/i>IqZ\u0394M15 Tn10 (TetR<\/sup>)<\/td>\n<\/tr>\n
TOP10<\/a><\/td>\n<\/td>\nF\u2013<\/sup> mcr<\/i>A \u0394(mrr<\/i>–hsd<\/i>RMS-mcr<\/i>BC) \u03a680lac<\/i>Z\u0394M15 \u0394lac<\/i>X74 rec<\/i>A1 ara<\/i>D139 \u0394(ara leu<\/i>) 7697 gal<\/i>U gal<\/i>K rps<\/i>L (StrR<\/sup>) end<\/i>A1 nup<\/i>G<\/td>\n<\/tr>\n
TOP10F\u2019<\/a><\/td>\n<\/td>\nF\u00b4{lac<\/i>Iq, Tn10<\/i>(TetR<\/sup>)} mcr<\/i>A \u0394(mrr<\/i>–hsd<\/i>RMS-mcr<\/i>BC) \u03a680lac<\/i>Z\u0394M15 \u0394lac<\/i>X74 rec<\/i>A1 ara<\/i>D139 \u0394(ara leu<\/i>) 7697 gal<\/i>U gal<\/i>K rps<\/i>L (StrR<\/sup>) end<\/i>A1 nup<\/i>G<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

P3\u30d7\u30e9\u30b9\u30df\u30c9\u3092\u4fdd\u6301\u3057\u3066\u3044\u308b\u5927\u8178\u83cc\u306e\u5897\u6b96\u306b\u3064\u3044\u3066<\/h2>\n

P3 \u30d7\u30e9\u30b9\u30df\u30c9\u3092\u6301\u3063\u3066\u3044\u308b\u5927\u8178\u83cc\u3067\u306f\u3001tRNA\u30b5\u30d7\u30ec\u30c3\u30b5\u30fc F\u907a\u4f1d\u5b50(sup<\/i>F)\u3092\u30b3\u30fc\u30c9\u3057\u3066\u3044\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u7dad\u6301\u3084\u9078\u629e\u304c\u53ef\u80fd\u3067\u3059\u3002P3\u306f\u4f4e\u30b3\u30d4\u30fc\u306e60 kb\u306e\u30a8\u30d4\u30bd\u30fc\u30e0\u30d7\u30e9\u30b9\u30df\u30c9\u3067\u3001\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\u3068\u3001\u30c6\u30c8\u30e9\u30b5\u30a4\u30af\u30ea\u30f3\u3068\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u305d\u308c\u305e\u308c\u306e\u8010\u6027\u907a\u4f1d\u5b50\u306e\u30a2\u30f3\u30d0\u30fc\u5909\u7570\u30df\u30e5\u30fc\u30bf\u30f3\u30c8\u3092\u6709\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001P3\u306e\u307f\u3092\u4fdd\u6301\u3057\u305f\u5927\u8178\u83cc\u306f\u3001\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u306b\u306f\u8010\u6027\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u30c6\u30c8\u30e9\u30b5\u30a4\u30af\u30ea\u30f3\u3001\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u306b\u306f\u611f\u53d7\u6027\u304c\u3042\u308a\u307e\u3059\u3002P3 \u30d7\u30e9\u30b9\u30df\u30c9\u3092\u6301\u3063\u305f\u5927\u8178\u83cc\u306bsup<\/i>F\u30d7\u30e9\u30b9\u30df\u30c9(\u4f8b\u3048\u3070pcDNA1.1\u3084pCM8\u306a\u3069)\u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a9\u30fc\u30e0\u3059\u308b\u3068\u3001\u305d\u308c\u3089\u306f\u30c6\u30c8\u30e9\u30b5\u30a4\u30af\u30ea\u30f3\u3001\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u3078\u306e\u8010\u6027\u3092\u7372\u5f97\u3057\u307e\u3059\u3002\u307e\u305f\u30a2\u30f3\u30d0\u30fc\u5909\u7570\u304c\u3082\u3068\u306b\u623b\u308b\u7387\u306f\u304b\u306a\u308a\u9ad8\u3044\u306e\u3067\u3001\u30c6\u30c8\u30e9\u30b5\u30a4\u30af\u30ea\u30f3\u3068\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u306e\u4e21\u65b9\u3067\u30bb\u30ec\u30af\u30b7\u30e7\u30f3\u3059\u308b\u3053\u3068\u304c\u91cd\u8981\u3067\u3059\u3002\u4fee\u5fa9\u3055\u308c\u305f\u5927\u8178\u83cc\u682a\u304c\u5897\u3048\u308b\u306e\u3092\u3055\u3051\u308b\u305f\u3081\u306b\u3001\u57f9\u5730\u306b\u306f\u3001\u6bd4\u8f03\u7684\u4f4e\u6fc3\u5ea6\u306e\u30c6\u30c8\u30e9\u30b5\u30a4\u30af\u30ea\u30f3(7.5 -10 \u03bcg\/ml)\u3068\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3(25-40 \u03bcg\/ml)\u3092\u52a0\u3048\u308b\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/p>\n

\u4e3b\u306a\u5927\u8178\u83cc\u306e\u907a\u4f1d\u5b50\u578b<\/h2>\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u907a\u4f1d\u5b50\u540d<\/th>\n<\/th>\n\u5185\u5bb9<\/th>\n<\/tr>\n
ara<\/i>-14<\/td>\n<\/td>\n\u30a2\u30e9\u30d3\u30ce\u30fc\u30b9\u306e\u4ee3\u8b1d\u304c\u3067\u304d\u306a\u3044\u3002<\/td>\n<\/tr>\n
arg<\/i>F<\/td>\n<\/td>\n\u30aa\u30eb\u30cb\u30c1\u30f3\u30ab\u30eb\u30d0\u30e2\u30a4\u30eb\u8ee2\u79fb\u9175\u7d20\u306e\u5909\u7570\u3002\u30a2\u30eb\u30ae\u30cb\u30f3\u306e\u4ee3\u8b1d\u3092\u963b\u5bb3\u3002<\/td>\n<\/tr>\n
dam\/dcm<\/i><\/td>\n<\/td>\nGATC\u914d\u5217\u4e2d\u306e\u30a2\u30c7\u30cb\u30f3\u306e\u30e1\u30c1\u30eb\u5316(dam<\/i>) \u307e\u305f\u306fCCWGG\u914d\u5217\u4e2d\u306e\u30b7\u30c8\u30b7\u30f3\u306e\u30e1\u30c1\u30eb\u5316(dcm<\/i>) \u304c\u8d77\u3053\u3089\u306a\u3044\u3088\u3046\u306a\u5909\u7570\u3002\u30e1\u30c1\u30eb\u5316\u306b\u3088\u308a\u6d3b\u6027\u306b\u5f71\u97ff\u3092\u53d7\u3051\u308b\u5236\u9650\u9175\u7d20(Ava<\/i>II\u3001Bcl<\/i>II\u306a\u3069)\u3067\u5207\u65ad\u3059\u308b\u305f\u3081\u306eDNA\u306e\u5897\u6b96\u306b\u5229\u7528\u3055\u308c\u308b\u3002<\/td>\n<\/tr>\n
DE3<\/td>\n<\/td>\nT7 RNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u3092\u30b3\u30fc\u30c9\u3059\u308b\u6eb6\u539f\u83cc\u3002T7-\u767a\u73fe\u7cfb\u3067\u306e\u8a98\u5c0e\u767a\u73fe\u306b\u7528\u3044\u3089\u308c\u308b\u3002<\/td>\n<\/tr>\n
deo<\/i>R<\/td>\n<\/td>\n\u30c7\u30aa\u30ad\u30b7\u30ea\u30dc\u30fc\u30b9\u5408\u6210\u306b\u95a2\u9023\u3057\u305f\u907a\u4f1d\u5b50\u306e\u6052\u5e38\u7684\u767a\u73fe\u3092\u53ef\u80fd\u306b\u3059\u308b\u5236\u5fa1\u907a\u4f1d\u5b50\u3002\u5927\u304d\u306aDNA\u3092\u52b9\u7387\u7684\u306b\u53d6\u308a\u8fbc\u307f\u306b\u304f\u304f\u3059\u308b\u3002<\/td>\n<\/tr>\n
end<\/i>A (end<\/i>A1)<\/td>\n<\/td>\n\u975e\u7279\u7570\u7684\u30a8\u30f3\u30c9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u3001\u30a8\u30f3\u30c9\u30cc\u30af\u30ec\u30a2\u30fc\u30bcI \u306e\u5909\u7570\u3002\u975e\u7279\u7570\u7684\u30a8\u30f3\u30c9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u6d3b\u6027\u3092\u9664\u3044\u305f\u306e\u3067\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u8abf\u88fd\u304c\u6539\u5584\u3055\u308c\u308b\u3002<\/td>\n<\/tr>\n
F’<\/td>\n<\/td>\nM13\u30d5\u30a1\u30fc\u30b8\u3067\u611f\u67d3\u3055\u305b\u305f\u969b\u306e\u4e00\u672c\u9396DNA\u306e\u751f\u6210\u306b\u7528\u3044\u3089\u308c\u308b\u3001\u81ea\u5df1\u4f1d\u64ad\u6027\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u3002F’\u306b\u306f\u305d\u306e\u7dad\u6301\u3092\u53ef\u80fd\u306b\u3059\u308b\u8010\u6027\u30de\u30fc\u30ab\u30fc\u3092\u542b\u3080\u3053\u3068\u304c\u591a\u304f\u3001lac<\/i>I\u304a\u3088\u3073lac<\/i>Z\u0394M15\u907a\u4f1d\u5b50\u578b\u3092\u4fdd\u6709\u3059\u308b\u3053\u3068\u304c\u591a\u3044\u3002<\/td>\n<\/tr>\n
gal<\/i>K<\/td>\n<\/td>\n\u30ac\u30e9\u30af\u30c8\u30ad\u30ca\u30fc\u30bc\u306e\u5909\u7570\u306b\u3088\u308a\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u306e\u4ee3\u8b1d\u304c\u3067\u304d\u306a\u3044\u3002gal<\/i>K-\u306e\u5927\u8178\u83cc\u306f\u30e9\u30af\u30c8\u30fc\u30b9\u3092\u552f\u4e00\u306e\u70ad\u7d20\u6e90\u3068\u3059\u308b\u74b0\u5883\u4e0b\u3067\u5897\u6b96\u3067\u304d\u308b\u3002<\/td>\n<\/tr>\n
gal<\/i>U<\/td>\n<\/td>\n\u30b0\u30eb\u30b3\u30fc\u30b9-1-\u30ea\u30f3\u9178\u30a6\u30ea\u30b8\u30ea\u30eb\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30e9\u30fc\u30bc\u306e\u7a81\u7136\u5909\u7570\u306b\u3088\u308a\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u304c\u5229\u7528\u3067\u304d\u306a\u3044\u3002gal<\/i>U-\u306e\u5927\u8178\u83cc\u306f\u30ac\u30e9\u30af\u30c8\u30fc\u30b9\u304c\u552f\u4e00\u306e\u70ad\u7d20\u6e90\u3067\u3042\u308b\u57f9\u990a\u6db2\u3067\u5897\u6b96\u3067\u304d\u308b\u3002<\/td>\n<\/tr>\n
gyr<\/i>A96<\/td>\n<\/td>\nDNA\u30b8\u30e3\u30a4\u30ec\u30fc\u30b9\u306e\u5909\u7570\u3002\u30ca\u30ea\u30b8\u30af\u30b9\u9178\u306b\u5bfe\u3057\u3066\u8010\u6027\u3092\u793a\u3059\u3002<\/td>\n<\/tr>\n
hsd<\/i><\/td>\n<\/td>\n\u5927\u8178\u83cc\u304c\u5916\u6765DNA\u3092\u8a8d\u8b58\u3059\u308b\u3053\u3068\u3092\u53ef\u80fd\u306b\u3059\u308b\u30e1\u30c1\u30eb\u5316\u304a\u3088\u3073\u5236\u9650\u5207\u65ad\u306e\u30b7\u30b9\u30c6\u30e0\u306e\u5909\u7570\u3002hsd<\/i>\u907a\u4f1d\u5b50\u578b\u306fPCR\u53cd\u5fdc\u306b\u3088\u3063\u3066\u751f\u6210\u3055\u308c\u305fDNA\u306e\u52b9\u7387\u7684\u306a\u30c8\u30e9\u30f3\u30b9\u30d5\u30a9\u30fc\u30e1\u30fc\u30b7\u30e7\u30f3\u3092\u53ef\u80fd\u306b\u3059\u308b\u3002
\n*hsd<\/i>R-\u975e\u30e1\u30c1\u30eb\u5316Eco<\/i>KI\u90e8\u4f4d\u306e\u5236\u9650\u5207\u65ad\u3092\u53d6\u308a\u9664\u304f(1)\u3002
\n**hsd<\/i>RMS-\u975e\u30e1\u30c1\u30eb\u5316Eco<\/i>KI\u90e8\u4f4d\u306e\u5236\u9650\u5207\u65ad\u3068Eco<\/i>KI\u90e8\u4f4d\u306e\u30e1\u30c1\u30eb\u5316\u3092\u540c\u6642\u306b\u53d6\u308a\u9664\u304f(1)\u3002
\n\u2020hsd<\/i>S-Eco<\/i>B\u90e8\u4f4d\u306e\u5236\u9650\u3068\u30e1\u30c1\u30eb\u5316\u3092\u540c\u6642\u306b\u53d6\u308a\u9664\u304f(2)\u3002<\/td>\n<\/tr>\n
lac<\/i>I(lac<\/i>Iq<\/sub>)<\/td>\n<\/td>\nlac<\/i>\u30aa\u30da\u30ec\u30fc\u30bf\u3092\u6709\u3059\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u304b\u3089\u306e\u767a\u73fe\u3092\u5236\u5fa1\u3059\u308blac<\/i>\u30ea\u30d7\u30ec\u30c3\u30b5\u30fc\u3092\u30b3\u30fc\u30c9\u3059\u308b\u3002IPTG\u304clac<\/i>\u30ea\u30d7\u30ec\u30c3\u30b5\u30fc\u3068\u7d50\u5408\u3057\u3066\u305d\u306e\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u6291\u5236\u3092\u89e3\u9664\u3059\u308b\u3002blue\/white\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3092\u884c\u3063\u305f\u308a\u3001\u7d44\u63db\u3048\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u3092\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u3059\u308b\u305f\u3081\u306b\u7528\u3044\u3089\u308c\u308b\u3053\u3068\u304c\u591a\u3044\u3002<\/td>\n<\/tr>\n
lac<\/i>y1<\/td>\n<\/td>\n\u03b2<\/i>-D-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u306e\u5909\u7570\u3002\u3053\u308c\u306b\u3088\u308a\u30e9\u30af\u30c8\u30fc\u30b9\u306e\u4ee3\u8b1d\u3092\u963b\u5bb3\u3002<\/td>\n<\/tr>\n
lac<\/i>Z<\/td>\n<\/td>\n\u03b2<\/i>-D-\u30ac\u30e9\u30af\u30c8\u30b7\u30c0\u30fc\u30bc\u907a\u4f1d\u5b50\u3002\u3053\u308c\u306b\u5909\u7570\u304c\u751f\u3058\u308b\u3068X-gal\u306e\u5b58\u5728\u4e0b\u3067\u7121\u8272(\u5909\u7570\u304c\u306a\u3044\u5834\u5408\u306f\u9752)\u306e\u30b3\u30ed\u30cb\u30fc\u304c\u751f\u6210\u3059\u308b<\/td>\n<\/tr>\n
lac<\/i>Z\u2206M15<\/td>\n<\/td>\nlac<\/i>Z\u907a\u4f1d\u5b50\u306e\u03b1-\u76f8\u88dc\u6027\u3092\u6709\u3057\u305f\u90e8\u5206\u6b20\u5931\u907a\u4f1d\u5b50\u3002\u7d44\u63db\u3048\u4f53\u306eblue\/white\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u306b\u7528\u3044\u3089\u308c\u308b\u3002\u901a\u5e38\u3001\u30e9\u30e0\u30c0\u72b6\u30d7\u30ed\u30d5\u30a1\u30fc\u30b8\u03c680\u307e\u305f\u306fF’\u4e0a\u306b\u5b58\u5728\u3059\u308b\u3002<\/td>\n<\/tr>\n
leu<\/i>B<\/td>\n<\/td>\n\u03b2<\/i>-\u30a4\u30bd\u30d7\u30ed\u30d4\u30eb\u30ea\u30f3\u30b4\u9178\u30c7\u30d2\u30c9\u30ed\u30b2\u30ca\u30fc\u30bc\u306e\u5909\u7570\u3002\u5897\u6b96\u306b\u306f\u6700\u5c11\u57f9\u5730\u306b\u30ed\u30a4\u30b7\u30f3\u306e\u6dfb\u52a0\u3092\u5fc5\u8981\u3068\u3059\u308b\u3002<\/td>\n<\/tr>\n
lon<\/i><\/td>\n<\/td>\nLon ATPase-\u4f9d\u5b58\u6027\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306e\u6b20\u640d\u3002\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u89e3\u3092\u6291\u5236\u3002B\u682a\u306f\u3059\u3079\u3066\u3053\u306e\u5909\u7570\u3092\u4fdd\u6709\u3059\u308b\u3002<\/td>\n<\/tr>\n
mcr<\/i>A, mcr<\/i>BC, mrr<\/i><\/td>\n<\/td>\n\u30e1\u30c1\u30eb\u5316DNA\u3092\u5916\u6765DNA\u3068\u3057\u3066\u8a8d\u8b58\u3067\u304d\u306a\u304f\u3059\u308b\u5909\u7570\u3002\u30b2\u30ce\u30e0DNA\u3084\u30e1\u30c1\u30eb\u5316cDNA\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u969b\u306b\u3053\u306e\u907a\u4f1d\u5b50\u578b\u304c\u5fc5\u8981\u3002<\/td>\n<\/tr>\n
nup<\/i>G<\/td>\n<\/td>\n\u30cc\u30af\u30ec\u30aa\u30b7\u30c9\u8f38\u9001\u7cfb\u306e\u5909\u7570\u3002<\/td>\n<\/tr>\n
omp<\/i>T<\/td>\n<\/td>\n\u5916\u90e8\u7d30\u80de\u819c\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u3092\u6b20\u3044\u305f\u5927\u8178\u83cc\u3002\u83cc\u682a\u5185\u3067\u767a\u73fe\u3055\u308c\u305f\u7570\u7a2e\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u89e3\u3092\u6291\u5236\u3059\u308b\u3002Omp<\/i>T–<\/sup>\u682a\u3067\u306f\u5206\u89e3\u3092\u53d7\u3051\u3066\u3044\u306a\u3044\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u56de\u53ce\u7387\u304c\u5411\u4e0a\u3059\u308b\u3002<\/td>\n<\/tr>\n
P3<\/td>\n<\/td>\n\u30a2\u30f3\u30d0\u30fc\u5909\u7570\u3092\u6709\u3057\u305f\u30a2\u30f3\u30d4\u30b7\u30ea\u30f3\u304a\u3088\u3073\u30c6\u30c8\u30e9\u30b5\u30a4\u30af\u30ea\u30f3\u8010\u6027\u907a\u4f1d\u5b50\u3092\u4fdd\u6709\u3059\u308b60 kb\u306e\u4f4e\u30b3\u30d4\u30fc\u30d7\u30e9\u30b9\u30df\u30c9\u3002\u4e3b\u306bsup<\/i>F-\u542b\u6709\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u9078\u629e\u306b\u7528\u3044\u3089\u308c\u308b\u3002\u30bb\u30ec\u30af\u30b7\u30e7\u30f3\u7528\u306e\u30ab\u30ca\u30de\u30a4\u30b7\u30f3\u8010\u6027\u907a\u4f1d\u5b50\u304c\u3042\u308b\u3002<\/td>\n<\/tr>\n
pLys<\/td>\n<\/td>\nT7\u30ea\u30be\u30c1\u30fc\u30e0\u3092\u30b3\u30fc\u30c9\u3059\u308b\u30d7\u30e9\u30b9\u30df\u30c9\u3002T7 RNA\u30dd\u30ea\u30e1\u30e9\u30fc\u30bc\u306e\u57fa\u5e95\u30ec\u30d9\u30eb\u3092\u6291\u5236\u3059\u308b\u3053\u3068\u306b\u3088\u308aT7-\u8a98\u5c0e\u767a\u73fe\u7cfb\u306b\u304a\u3051\u308b\u57fa\u5e95\u767a\u73fe\u3092\u6291\u5236\u3059\u308b\u305f\u3081\u306b\u7528\u3044\u3089\u308c\u308b\u3002<\/td>\n<\/tr>\n
pro<\/i>AB<\/td>\n<\/td>\n\u6700\u5c11\u57f9\u5730\u306b\u30d7\u30ed\u30ea\u30f3\u306e\u6dfb\u52a0\u304c\u5fc5\u8981\u3002<\/td>\n<\/tr>\n
rec<\/i>A<\/td>\n<\/td>\nDNA\u306e\u4e00\u822c\u7684\u306a\u7d44\u63db\u3048\u306b\u95a2\u4e0e\u3059\u308b\u907a\u4f1d\u5b50\u306e\u5909\u7570\u3002\u5c0e\u5165DNA\u3068\u5bbf\u4e3bDNA\u3068\u306e\u7d44\u63db\u3048\u3092\u9632\u3050\u3002\u7279\u306b\u30c0\u30a4\u30ec\u30af\u30c8\u30ea\u30d4\u30fc\u30c8\u3092\u6709\u3059\u308b\u907a\u4f1d\u5b50\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306b\u6709\u7528\u3002<\/td>\n<\/tr>\n
rel<\/i>A<\/td>\n<\/td>\n\u30bf\u30f3\u30d1\u30af\u8cea\u5408\u6210\u304c\u884c\u308f\u308c\u305a\u306bRNA\u304c\u5408\u6210\u3055\u308c\u308b(\u5f1b\u7de9\u8868\u73fe\u578b)\u3002rel<\/i>A\u907a\u4f1d\u5b50\u5ea7\u306f\u8ee2\u5199\u3068\u7ffb\u8a33\u9593\u306e\u30ab\u30c3\u30d7\u30ea\u30f3\u30b0\u3092\u8abf\u7bc0\u3059\u308b\u3002\u91ce\u751f\u578b\u3067\u306f\u30a2\u30df\u30ce\u9178\u6fc3\u5ea6\u304c\u5236\u9650\u3055\u308c\u308b\u3068RNA\u5408\u6210\u304c\u505c\u6b62\u3059\u308b(\u7dca\u7e2e\u5fdc\u7b54\u3068\u3057\u3066\u3082\u77e5\u3089\u308c\u308b)\u3002rel<\/i>A\u682a(\u5f1b\u7de9\u5fdc\u7b54)\u3067\u306f\u30a2\u30df\u30ce\u9178\u6fc3\u5ea6\u304c\u5236\u9650\u3055\u308c\u3066\u3044\u3066\u3082RNA\u306e\u5408\u6210\u304c\u7d99\u7d9a\u3059\u308b\u3002<\/td>\n<\/tr>\n
rsp<\/i>L<\/td>\n<\/td>\n30S \u30ea\u30dc\u30bd\u30fc\u30e0\u306eS12 \u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u5909\u7570\u3002\u30b9\u30c8\u30ec\u30d7\u30c8\u30de\u30a4\u30b7\u30f3\u306b\u5bfe\u3059\u308b\u8010\u6027\u304c\u5f97\u3089\u308c\u308b\u3002<\/td>\n<\/tr>\n
sup<\/i>E,F<\/td>\n<\/td>\n\u30a2\u30f3\u30d0\u30fc(sup<\/i>E)(UAG)\u307e\u305f\u306f\u30c1\u30ed\u30b7\u30f3(sup<\/i>F)\u306etRNA\u30b0\u30eb\u30bf\u30df\u30f3\u30b5\u30d7\u30ec\u30c3\u30b5\u30fc\u3002<\/td>\n<\/tr>\n
thi<\/i>-1<\/td>\n<\/td>\n\u6700\u5c11\u57f9\u5730\u306b\u30c1\u30a2\u30df\u30f3\u306e\u6dfb\u52a0\u3092\u8981\u6c42\u3059\u308b\u3002<\/td>\n<\/tr>\n
Tn10<\/td>\n<\/td>\n\u30c8\u30e9\u30f3\u30b9\u30dd\u30be\u30f3\u3092\u4ecb\u3057\u305f\u30c6\u30c8\u30e9\u30b5\u30a4\u30af\u30ea\u30f3\u8010\u6027<\/td>\n<\/tr>\n
ton<\/i>A<\/td>\n<\/td>\n\u6eb6\u83cc\u6027\u30d0\u30af\u30c6\u30ea\u30aa\u30d5\u30a1\u30fc\u30b8\u306eT1\u3001T5\u304a\u3088\u3073\u03c680\u306b\u5bfe\u3059\u308b\u8010\u6027\u3092\u4ed8\u4e0e\u3059\u308b\u3002<\/td>\n<\/tr>\n
tra<\/i>D, D36<\/td>\n<\/td>\n\u8ee2\u79fb\u56e0\u5b50\u306e\u5909\u7570\u306b\u3088\u308aF’\u30a8\u30d4\u30bd\u30fc\u30e0\u306e\u8ee2\u79fb\u3092\u59a8\u3052\u308b<\/td>\n<\/tr>\n
tsx<\/i><\/td>\n<\/td>\nT6\u30d5\u30a1\u30fc\u30b8\u304a\u3088\u3073\u30b3\u30ea\u30b7\u30f3K\u306b\u5bfe\u3059\u308b\u8010\u6027\u3092\u4ed8\u4e0e\u3059\u308b\u3002<\/td>\n<\/tr>\n
xyl<\/i>-5<\/td>\n<\/td>\n\u30ad\u30b7\u30ed\u30fc\u30b9\u306e\u4ee3\u8b1d\u306e\u963b\u5bb3\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

(1) Eco<\/i>KI\u8a8d\u8b58\u90e8\u4f4d: CC(N6<\/sub>)GTGC \u3082\u3057\u304f\u306f GCAC(N6<\/sub>)GTT<\/span>
\n(2) Eco<\/i>B\u8a8d\u8b58\u90e8\u4f4d: TGA(N8<\/sub>)TGCT<\/span><\/p>\n

\u304a\u308f\u308a\u306b<\/h2>\n

\u3044\u304b\u304c\u3067\u3057\u305f\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

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