{"id":11454,"date":"2022-05-25T09:55:31","date_gmt":"2022-05-25T00:55:31","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=11454"},"modified":"2022-06-16T17:07:24","modified_gmt":"2022-06-16T08:07:24","slug":"gene-editing_bid_crispr-ts3","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_crispr-ts3\/","title":{"rendered":"CRISPR\/Cas9\u3092\u7528\u3044\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u3001\u304a\u3088\u3073\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u3088\u308b\u7de8\u96c6\u52b9\u7387\u78ba\u8a8d\u306e\u91cd\u8981\u6027"},"content":{"rendered":"

\u3053\u306e10\u5e74\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u6280\u8853\u306f\u3001\u3081\u307e\u3050\u308b\u3057\u3044\u767a\u5c55\u3092\u9042\u3052\u307e\u3057\u305f\u3002CRISPR (Clustered Regulatory Interspaced Short Palindromic Repeats)\u306e\u3088\u3046\u306a\u6280\u8853\u306f\u3001\u75c5\u7406\u5b66\u7684\u57fa\u76e4\u306e\u89e3\u660e\u3060\u3051\u3067\u306f\u306a\u304f\u3001HIV\u3001SARS-CoV-2\u306a\u3069\u306e\u611f\u67d3\u75c7\u3084\u3001\u5148\u5929\u6027\u75be\u60a3\u306e\u6cbb\u7642\u6cd5\u958b\u767a\u306b\u307e\u3067\u53ef\u80fd\u6027\u3092\u5e83\u3052\u307e\u3057\u305f\u3002DNA\u306e\u4fee\u98fe\u304c\u7d30\u80de\u306e\u6a5f\u80fd\u306b\u53ca\u307c\u3059\u5f71\u97ff\u3092\u8003\u616e\u3059\u308b\u3068\u3001\u6b63\u78ba\u3067\u4fe1\u983c\u6027\u306e\u9ad8\u3044\u30c4\u30fc\u30eb\u3092\u7528\u3044\u3066\u3001\u3053\u306e\u3088\u3046\u306a\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u6027\u8cea\u3092\u691c\u8a3c\u3057\u3001\u307e\u305f\u7406\u89e3\u3059\u308b\u3053\u3068\u304c\u975e\u5e38\u306b\u91cd\u8981\u3067\u3059\u3002<\/p>\n

\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u3088\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u691c\u8a3c\u30c4\u30fc\u30eb Applied Biosystems\u2122 SeqScreener Gene Edit Confirmation App\u3064\u3044\u3066\u3001\u3054\u7d39\u4ecb\u3044\u305f\u3057\u307e\u3059\u3002<\/p>\n

\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01
\n\u30fb\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u5b9f\u65bd\u3055\u308c\u3066\u3044\u308b\u65b9<\/p>\n

CRISPR\u306e\u51fa\u73fe<\/h2>\n

\u3053\u308c\u307e\u3067\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u306fTALENs (Transcription activator-like effector nucleases) \u3084 ZFNs (zinc finger nucleases)\u306a\u3069\u4ed6\u306e\u624b\u6cd5\u304c\u4f7f\u308f\u308c\u3066\u304d\u307e\u3057\u305f\u3002CRISPR\u306f\u305d\u308c\u3088\u308a\u3082\u65b0\u3057\u3044\u3001\u30b7\u30f3\u30d7\u30eb\u306a\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u624b\u6cd5\u3067\u3059\u30022020\u5e74\u306b\u306f\u3001Emmanuelle Charpentier\u3068\u3001Jennifer Doudna\u304c\u3001Cas9\u3092\u4ecb\u3057\u305fDNA\u4fee\u98fe\u30b7\u30b9\u30c6\u30e0\u306e\u751f\u5316\u5b66\u7684\u7279\u6027\u8a55\u4fa1\u3067\u30ce\u30fc\u30d9\u30eb\u5316\u5b66\u8cde\u3092\u53d7\u8cde\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u672c\u6765CRISPR\u306f\u3001\u30d0\u30af\u30c6\u30ea\u30a2\u3084\u4ed6\u306e\u539f\u6838\u751f\u7269\u304c\u30a6\u30a4\u30eb\u30b9\u3084\u30d7\u30e9\u30b9\u30df\u30c9\u304b\u3089\u8eab\u3092\u5b88\u308b\u305f\u3081\u306b\u7528\u3044\u308b\u3001\u9069\u5fdc\u7684\u9632\u885b\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u3053\u3068\u3067\u3059\u3002\u7aef\u7684\u306b\u8a00\u3046\u3068\u3001\u5916\u6765DNA\u306e\u77ed\u3044\u914d\u5217\uff08\u30b9\u30da\u30fc\u30b5\u30fc\uff09\u304c\u5bbf\u4e3b\u306e\u30b2\u30ce\u30e0\u306e\u7e70\u308a\u8fd4\u3057\u914d\u5217\uff08\u30ea\u30d4\u30fc\u30c8\uff09\u306b\u96a3\u63a5\u3057\u3001\u305d\u306e\u77ed\u3044\u914d\u5217\u3092\u5bbf\u4e3b\u30b2\u30ce\u30e0\u306b\u53d6\u308a\u8fbc\u3080\u3068\u3044\u3046\u30e1\u30ab\u30cb\u30ba\u30e0\u3067\u3059\u3002<\/p>\n

\u305d\u306e\u30b9\u30da\u30fc\u30b5\u30fc\u914d\u5217\u306f\u3001\u8ee2\u5199\u3055\u308c\u3066small RNA\u306b\u306a\u308b\u3068\u3001\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\uff08Cas9\uff09\u3068\u6a5f\u80fd\u7684\u306a\u8907\u5408\u4f53\u3092\u5f62\u6210\u3057\u3001\u5916\u6765DNA\u3092\u8a8d\u8b58\u3001\u6d88\u5316\u3057\u307e\u3059\u3002\u3053\u306e\u30e1\u30ab\u30cb\u30ba\u30e0\u306f\u3001\u5f8c\u306b\u771f\u6838\u751f\u7269\u306e\u30b2\u30ce\u30e0\u306b\u3082\u9069\u5fdc\u3055\u308c\u30012013\u5e74\u306b\u521d\u3081\u3066\u3001\u30d2\u30c8\u3068\u30de\u30a6\u30b9\u306e\u7d30\u80de\u3067\u6539\u5909\u304c\u8d77\u304d\u305f\u3053\u3068\u304c\u78ba\u8a8d\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n

\u907a\u4f1d\u5b50\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u3068\u3059\u308b\u306b\u306f\u3001\u305d\u306e\u76ee\u7684\u9818\u57df\u3068\u76f8\u88dc\u6027\u914d\u5217\u3092\u6301\u3064\u3001\u30ac\u30a4\u30c9RNA\uff08gRNA\uff09\u3092\u8a2d\u8a08\u3057\u307e\u3059\u3002\u3053\u306egRNA\u306f\u3001CRISPR\/Cas9\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u8907\u5408\u4f53\u3092\u7279\u5b9a\u306e\u914d\u5217\u306b\u8a98\u5c0e\u3057\u307e\u3059\u3002\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306b\u3088\u3063\u3066\u5207\u65ad\u3055\u308c\u305f\u90e8\u4f4d\u306f\u3001\u975e\u76f8\u540c\u672b\u7aef\u7d50\u5408\u3084\u92f3\u578b\u6307\u5411\u6027\u4fee\u5fa9\u306b\u3088\u3063\u3066\u4fee\u5fa9\u304c\u306a\u3055\u308c\u307e\u3059\u3002TALEN\u304c\u6a19\u7684\u3068\u306a\u308bDNA\u914d\u5217\u306b\u5408\u308f\u305b\u3001\u7279\u7570\u7684\u306a\u8ee2\u5199\u6d3b\u6027\u5316\u56e0\u5b50\u69d8transcription activator-like\uff08TAL\uff09\u30a8\u30d5\u30a7\u30af\u30bf\u30fc\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5fc5\u8981\u3068\u3059\u308b\u306e\u306b\u5bfe\u3057\u3001CRISPR\u306f\u30ac\u30a4\u30c9RNA\u914d\u5217\u3092\u5207\u308a\u66ff\u3048\u308b\u3060\u3051\u306a\u306e\u3067\u3001\u907a\u4f1d\u5b50\u7de8\u96c6\u306e\u5fdc\u7528\u306b\u306fCRISPR\u306e\u65b9\u304c\u306f\u308b\u304b\u306b\u5bb9\u6613\u3067\u3059\u3002<\/p>\n

\u75be\u60a3\u7814\u7a76\u306b\u304a\u3051\u308bCRISPR\u3092\u7528\u3044\u305f\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/h2>\n

\u907a\u4f1d\u5b50\u75be\u60a3\u30fb\u611f\u67d3\u75c7
\n\u7b4b\u30b8\u30b9\u30c8\u30ed\u30d5\u30a3\u30fc<\/strong>
\nCRISPR\u3092\u7528\u3044\u305f\u3044\u304f\u3064\u304b\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306f\u3001\u8eab\u4f53\u306e\u6a5f\u80fd\u3092\u8457\u3057\u304f\u640d\u306a\u3046\u75be\u60a3\u3001\u91cd\u7be4\u3067\u751f\u547d\u3092\u8105\u304b\u3059\u75be\u60a3\u306e\u7814\u7a76\u306b\u304a\u3044\u3066\u5b9f\u8a3c\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4f8b\u3048\u3070\u3001Bengtsson\u3089\u306f\u3001CRISPR\/Cas9\u3092\u7b4b\u8089\u306b\u7279\u7570\u7684\u306b\u767a\u73fe\u3055\u305b\u308b\u3053\u3068\u3067\u3001\u30c7\u30e5\u30b7\u30a7\u30f3\u30cc\u578b\u7b4b\u30b8\u30b9\u30c8\u30ed\u30d5\u30a3\u30fc\uff08DMD\uff09\u306e\u52d5\u7269\u30e2\u30c7\u30eb\u306b\u304a\u3044\u3066\u3001\u907a\u4f1d\u5b50\u5909\u7570\u306e\u4fee\u6b63\u306b\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u304c\u5fdc\u7528\u3067\u304d\u308b\u3053\u3068\u3092\u793a\u3057\u307e\u3057\u305f\u3002[2]
\n\u907a\u4f1d\u5b50\u304c\u6539\u5909\u3055\u308c\u305f\u30de\u30a6\u30b9\u3067\u306f\u3001\u7b4b\u8089\u306b\u30b8\u30b9\u30c8\u30ed\u30d5\u30a3\u30f3\u304c\u3057\u3063\u304b\u308a\u3068\u767a\u73fe\u3057\u3001\u75be\u60a3\u306b\u95a2\u9023\u3057\u305f\u8868\u73fe\u578b\u304c\u6539\u5584\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n

\u3053\u306e\u554f\u984c\u3092\u89e3\u6c7a\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u9ad8\u3044\u611f\u5ea6\u3068\u7279\u7570\u6027\u3092\u6301\u3064\u8a3a\u65ad\u30c6\u30b9\u30c8\u304c\u9375\u3068\u306a\u308a\u307e\u3059\u3002\u611f\u5ea6\u30fb\u7279\u7570\u6027\u3068\u306f\u3001\u3042\u308b\u52d5\u7269\u304c\u75c5\u6c17\u3067\u3042\u308b\u304b\u3001\u75c5\u6c17\u3067\u306a\u3044\u304b\u3092\u6b63\u3057\u304f\u5206\u985e\u3059\u308b\u8a3a\u65ad\u30c6\u30b9\u30c8\u306e\u80fd\u529b\u3092\u793a\u3059\u3082\u306e\u3067\u3059\u3002<\/p>\n

HIV<\/strong>
\n\u307e\u305f\u3001CRISPR\u306f\u907a\u4f1d\u5b50\u75be\u60a3\u3060\u3051\u3067\u306a\u304f\u3001HIV\u611f\u67d3\u75c7\u306e\u6cbb\u7642\u6226\u7565\u3068\u3057\u3066\u3082\u671f\u5f85\u3055\u308c\u3066\u3044\u307e\u3059\u3002HIV\u306e\u6cbb\u7652\u3092\u5b9f\u73fe\u3059\u308b\u306b\u3042\u305f\u308a\u5927\u304d\u306a\u30cd\u30c3\u30af\u3068\u306a\u308b\u306e\u306f\u3001\u30a6\u30a4\u30eb\u30b9\u304c\u5bbf\u4e3b\u306e\u30b2\u30ce\u30e0\u306b\u7d44\u307f\u8fbc\u307e\u308c\u308b\u305f\u3081\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u8907\u88fd\u3092\u6291\u5236\u3059\u308b\u305f\u3081\u306b\u3001\u611f\u67d3\u8005\u306f\u751f\u6daf\u306b\u308f\u305f\u3063\u3066\u6297\u30a6\u30a4\u30eb\u30b9\u6cbb\u7642\u3092\u53d7\u3051\u306a\u3051\u308c\u3070\u306a\u3089\u306a\u3044\u3053\u3068\u3067\u3059\u3002\u6700\u8fd1\u306e\u30b5\u30eb\u3067\u306e\u7814\u7a76\u3067\u306f\u3001Cas9\u304cHIV\u3068\u8fd1\u7e01\u306e\u30a6\u30a4\u30eb\u30b9\u3067\u3042\u308b\u30b7\u30df\u30a2\u30f3\u514d\u75ab\u4e0d\u5168\u30a6\u30a4\u30eb\u30b9\uff08SIV\uff09\u306e\u30d7\u30ed\u30a6\u30a4\u30eb\u30b9DNA\u3092\u3001\u611f\u67d3\u3057\u305f\u8840\u6db2\u7d30\u80de\u3060\u3051\u3067\u306a\u304f\u3001\u30a6\u30a4\u30eb\u30b9\u306e\u7d44\u7e54\u30ea\u30b6\u30fc\u30d0\u30fc\u304b\u3089\u3082\u9664\u53bb\u3057\u3001\u305d\u306e\u8907\u88fd\u3092\u9632\u3052\u308b\u52b9\u679c\u304c\u3042\u308b\u3053\u3068\u304c\u793a\u3055\u308c\u307e\u3057\u305f[3]<\/p>\n

CRISPR\/Cas9\u306e\u7d99\u7d9a\u7684\u306a\u6539\u826f<\/strong>
\nCRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u65b0\u305f\u306a\u554f\u984c\u306b\u5bfe\u3057\u3066\u6539\u826f\u304c\u7d9a\u3051\u3089\u308c\u3066\u3044\u307e\u3059\u3002Cas12a\u3001Cas12b\u306a\u3069\u306e\u30ac\u30a4\u30c9RNA\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u3092\u542b\u3080\u3001\u3044\u304f\u3064\u304b\u306e\u65b0\u3057\u3044\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u304c\u767a\u898b\u30fb\u6700\u9069\u5316\u3055\u308c\u3001\u30b5\u30a4\u30ba\u304c\u5c0f\u3055\u3044\u3053\u3068\u3084\u3001\u30d7\u30ed\u30c8\u30b9\u30da\u30fc\u30b5\u30fc\u96a3\u63a5\u30e2\u30c1\u30fc\u30d5\uff08PAM\uff09\u914d\u5217\u306a\u3069\u306e\u7279\u5fb4\u3068\u3068\u3082\u306b\u3001\u6a19\u7684\u306e\u9078\u629e\u304c\u62e1\u5927\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

CRISPR\u306b\u3088\u308bSARS-CoV-2\u304a\u3088\u3073\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6A\u306e\u30b2\u30ce\u30e0\u6539\u5909\u7814\u7a76<\/strong>
\n\u3053\u308c\u3089\u306e\u30cc\u30af\u30ec\u30a2\u30fc\u30bc\u306e\u5f37\u529b\u306a\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306e1\u3064\u306f\u3001\u3044\u304f\u3064\u304b\u306e\u547c\u5438\u5668\u7cfb\u75c5\u539f\u4f53\u306e\u30b2\u30ce\u30e0\u3092\u542b\u3080RNA\u30a6\u30a4\u30eb\u30b9\u30b2\u30ce\u30e0\u3092\u76f4\u63a5\u6a19\u7684\u3068\u3057\u3001\u6539\u5909\u3059\u308b\u3053\u3068\u3067\u3059\u3002Abbott\u3089\u306f\u3001\u6700\u8fd1\u3053\u306e\u6226\u7565\u3092\u7528\u3044\u3066\u3001SARS-CoV-2\u304a\u3088\u3073\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6A\u30a6\u30a4\u30eb\u30b9\u306eRNA\u30b2\u30ce\u30e0\u3092\u6d88\u5316\u3057\u3001\u30d2\u30c8\u80ba\u4e0a\u76ae\u7d30\u80de\u306b\u304a\u3051\u308b\u30a6\u30a4\u30eb\u30b9\u8907\u88fd\u3092\u963b\u5bb3\u3059\u308b\u3001\u6982\u5ff5\u5b9f\u8a3c\u7814\u7a76\u3092\u767a\u8868\u3057\u307e\u3057\u305f[4]\u3002<\/p>\n

Related: Sanger Sequencing Solutions for SARS-CoV-2 Research<\/a><\/p>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u304a\u3051\u308b\u7de8\u96c6\u52b9\u7387\u306e\u8a55\u4fa1\u306e\u91cd\u8981\u6027<\/h3>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u884c\u3046\u969b\u3001\u7de8\u96c6\u3092\u6210\u529f\u3055\u305b\u308b\u305f\u3081\u306e\u3044\u304f\u3064\u304b\u306e\u8003\u616e\u3059\u3079\u304d\u70b9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002
\n\u30fb\u30ac\u30a4\u30c9RNA\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u3001\u30bf\u30fc\u30b2\u30c6\u30a3\u30f3\u30b0\u306e\u52b9\u7387\u306f\u7570\u306a\u308b
\n\u30fbCRISPR\/Cas9\u8907\u5408\u4f53\u3092\u7d30\u80de\u5185\u306b\u5c0e\u5165\u3059\u308b\u305f\u3081\u306b\u3001\u3055\u307e\u3056\u307e\u306a\u65b9\u6cd5\u304c\u5229\u7528\u53ef\u80fd\u3067\u3042\u308b
\n\uff08\u88fd\u54c1\u306e\u8a73\u7d30\u306f\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\uff09
\n\u30fb\u7d30\u80de\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u3001\u4fee\u5fa9\u52b9\u7387\u306f\u7570\u306a\u308b
\n\u30fb\u6700\u5f8c\u306b\u3001\u4fee\u5fa9\u6a5f\u69cb\u305d\u306e\u3082\u306e\u304c\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u5468\u8fba\u914d\u5217\u306b\u304a\u3044\u3066\u6df7\u5408\u914d\u5217\u3092\u4f5c\u308a\u51fa\u3059<\/p>\n

\u3086\u3048\u306b\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u304a\u3044\u3066\u306f\u3001\u5b9f\u9a13\u6761\u4ef6\u4e0b\u3067\u306e\u7de8\u96c6\u52b9\u7387\u3092\u898b\u6975\u3081\u308b\u3053\u3068\u304c\u975e\u5e38\u306b\u91cd\u8981\u3067\u3059\u3002<\/p>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u306b\u304a\u3051\u308b\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9<\/h3>\n

\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u306b\u304a\u3044\u3066\u7de8\u96c6\u52b9\u7387\u306e\u5224\u5b9a\u306b\u6709\u7528\u306a\u30c4\u30fc\u30eb\u3067\u3059\u3002\u6cbb\u7642\u5f8c\u306e\u521d\u4ee3\u7d30\u80de\u96c6\u56e3\u304b\u3089\u30d0\u30eb\u30afDNA\u3092\u62bd\u51fa\u3057\u3001\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3092\u5b9f\u65bd\u3057\u307e\u3059\u3002
\n\u3053\u306e\u521d\u4ee3\u7d30\u80de\u96c6\u56e3\u306b\u306f\u3001\u7de8\u96c6\u3055\u308c\u3066\u3044\u306a\u3044\u914d\u5217\u3068\u3001\u7de8\u96c6\u3055\u308c\u30d8\u30c6\u30ed\u914d\u5217\u3092\u542b\u3080\u7d30\u80de\u304c\u6df7\u5728\u3057\u3066\u3044\u308b\u72b6\u614b\u3067\u3059\u3002\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u5f97\u3089\u308c\u305f\u3053\u306e\u6df7\u5408\u96c6\u56e3\u306e\u6ce2\u5f62\u3092\u898b\u308b\u3068\u3001\u7de8\u96c6\u3055\u308c\u305f\u30b2\u30ce\u30e0\u3068\u7de8\u96c6\u3055\u308c\u3066\u3044\u306a\u3044\u30b2\u30ce\u30e0\u304c\u591a\u6570\u3042\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3059\u3002\u96c6\u56e3\u5185\u306e\u6b63\u78ba\u306a\u914d\u5217\u306e\u8b58\u5225\u306f\u96e3\u3057\u3044\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u304c\u3001\u3059\u3079\u3066\u306e\u975e\u91ce\u751f\u578b\u914d\u5217\u306e\u76f8\u5bfe\u7684\u306a\u91cf\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u306e\u5224\u5b9a\u306b\u5f79\u7acb\u3064\u60c5\u5831\u3067\u3059\u3002<\/p>\n

\u7de8\u96c6\u52b9\u7387\u304c\u5341\u5206\u306b\u9ad8\u3044\u5834\u5408\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u306e\u6b21\u306e\u30b9\u30c6\u30c3\u30d7\u3068\u3057\u3066\u306f\u3001\u76ee\u7684\u306e\u7de8\u96c6\u304c\u65bd\u3055\u308c\u305f\u7d30\u80de\u3092\u5206\u96e2\u3055\u305b\u308b\u3053\u3068\u3067\u3059\u3002\u65b9\u6cd5\u3068\u3057\u3066\u306f\u3001\u521d\u4ee3\u57f9\u990a\u7d30\u80de\u3092\u9650\u754c\u5e0c\u91c8\u3057\u3066\u5358\u4e00\u7d30\u80de\u306b\u3057\u3001\u305d\u306e\u7d30\u80de\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3067\u5897\u6b96\u3055\u305b\u308b\u306e\u304c\u4e00\u822c\u7684\u3067\u3059\u3002\u305d\u306e\u5f8c\u3001\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3092\u7528\u3044\u3001\u5897\u5e45\u3055\u305b\u305f\u5404\u7d30\u80de\u306b\u304a\u3051\u308b\u7de8\u96c6\u9818\u57df\u5468\u8fba\u306e\u907a\u4f1d\u5b50\u7de8\u96c6\u306e\u6709\u7121\u3092\u78ba\u8a8d\u3057\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u3063\u3066\u3001\u7d30\u80de\u96c6\u56e3\u306e\u6b63\u78ba\u306a\u914d\u5217\u304c\u5f97\u3089\u308c\u3001\u4e0b\u6d41\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u5229\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u95a2\u9023Page: \u30ad\u30e3\u30d4\u30e9\u30ea\u30fc\u96fb\u6c17\u6cf3\u52d5\u3092\u7528\u3044\u305f CRISPR \u304a\u3088\u3073 TALEN \u907a\u4f1d\u5b50\u7de8\u96c6\u306e\u78ba\u8a8d<\/a><\/p>\n

\u65b0\u3057\u3044\u30c4\u30fc\u30eb\u306b\u3088\u308b\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u7c21\u7d20\u5316<\/h3>\n

\u3088\u308a\u591a\u304f\u306e\u60c5\u5831\u3068\u3001\u3088\u308a\u512a\u308c\u305f\u753b\u50cf
\n\u7de8\u96c6\u52b9\u7387\u306f\u3001\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u6ce2\u5f62\u304b\u3089\u76ee\u8996\u3067\u78ba\u8a8d\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u304c\u3001\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u30c4\u30fc\u30eb\u3092\u4f7f\u7528\u3059\u308b\u3053\u3068\u3067\u3001\u3088\u308a\u591a\u304f\u306e\u60c5\u5831\u3068\u3088\u308a\u512a\u308c\u305f\u753b\u50cf\u3092\u53d6\u5f97\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u30c7\u30fc\u30bf\u89e3\u6790\u3092\u8fc5\u901f\u306b\u884c\u3046\u305f\u3081\u306b\u3001\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u306f\u3001\u65b0\u3057\u3044\u907a\u4f1d\u5b50\u7de8\u96c6\u30bd\u30d5\u30c8\u30a6\u30a7\u30a2\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3001\u300cSeqScreener Gene Edit Confirmation App<\/a> \u300d\u3092\u958b\u767a\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u6a5f\u80fd\u304c\u8c4a\u5bcc\u306a\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2<\/h3>\n

\u9818\u57df\u3068\u983b\u5ea6
\nSeqScreener Gene Edit Confirmation App\u3067\u306f\u3001\u521d\u4ee3\u57f9\u990a\u7d30\u80de\u3001\u3082\u3057\u304f\u306f\u4e8c\u6b21\u57f9\u990a\u7d30\u80de\u306b\u304a\u3051\u308b\u3001\u633f\u5165\u30fb\u6b20\u640d\u306e\u7bc4\u56f2\u3068\u983b\u5ea6\u306e\u4e21\u65b9\u306e\u691c\u51fa\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n

\u8996\u899a\u5316
\n\u633f\u5165\u3084\u6b20\u5931\u3092\u4e00\u5ea6\u306b\u53ef\u8996\u5316\u3067\u304d\u308bPlate View\u30aa\u30d7\u30b7\u30e7\u30f3\u3082\u5099\u3048\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u7d30\u80de\u96c6\u56e3\u306b\u304a\u3051\u308b\u7de8\u96c6\u52b9\u7387\u3084\u7de8\u96c6\u30d1\u30bf\u30fc\u30f3\u3082\u8868\u793a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002\u7279\u306b\u3001\u8907\u6570\u306egRNA\u3084\u7d30\u80de\u306e\u7a2e\u985e\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u624b\u6cd5\u306e\u8a55\u4fa1\u3092\u4f34\u3046\u3088\u3046\u306a\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306a\u5b9f\u9a13\u306b\u6709\u52b9\u3067\u3059\u3002<\/p>\n

\u7de8\u96c6\u52b9\u7387
\nSeqScreener Gene Edit Confirmation App\u306f\u3001\u975e\u76f8\u540c\u672b\u7aef\u7d50\u5408\u306b\u3088\u3063\u3066\u751f\u3058\u305f\u3001\u633f\u5165\u30fb\u6b20\u640d\u306e\u52b9\u7387\u3092\u5224\u5b9a\u3059\u308b\u6a5f\u80fd\u3060\u3051\u3067\u306a\u304f\u3001SNP\u306e\u5909\u66f4\u306a\u3069\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u4f9d\u5b58\u3057\u305f\u7de8\u96c6\u306e\u52b9\u7387\u3082\u5224\u5b9a\u3059\u308b\u3053\u3068\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n

\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8
\n\u6700\u5f8c\u306b\u3001SeqScreener Gene Edit Confirmation App\u306f\u6700\u592796\u30b5\u30f3\u30d7\u30eb\u3092\u540c\u6642\u306b\u89e3\u6790\u3067\u304d\u308b\u3088\u3046\u8a2d\u8a08\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u30d0\u30c3\u30c1\u30a2\u30c3\u30d7\u30ed\u30fc\u30c9\u6a5f\u80fd\u306b\u3088\u308a\u3001\u30cf\u30a4\u30b9\u30eb\u30fc\u30d7\u30c3\u30c8\u306a\u30e9\u30dc\u3067\u3082\u89e3\u6790\u304c\u5bb9\u6613\u3067\u3059\u3002<\/p>\n

\"SeqScreener\"SeqScreener<\/p>\n

CRISPR\/Cas9\u3068\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u3088\u308b\u907a\u4f1d\u5b50\u7de8\u96c6\u306e\u78ba\u8a8d<\/h2>\n

CRISPR\u306e\u3088\u3046\u306a\u907a\u4f1d\u5b50\u7de8\u96c6\u6280\u8853\u306f\u3001\u533b\u7642\u306b\u304a\u3044\u3066\u5927\u304d\u306a\u53ef\u80fd\u6027\u3092\u79d8\u3081\u3066\u304a\u308a\u3001\u3044\u304f\u3064\u304b\u306e\u75be\u60a3\u306b\u5bfe\u3057\u5b9f\u65bd\u53ef\u80fd\u306a\u6cbb\u7642\u30aa\u30d7\u30b7\u30e7\u30f3\u3068\u3057\u3066\u691c\u8a0e\u3055\u308c\u59cb\u3081\u305f\u3070\u304b\u308a\u3067\u3059\u3002\u3053\u306e\u5206\u91ce\u3078\u306e\u95a2\u5fc3\u304c\u9ad8\u307e\u308b\u4e2d\u3001\u7814\u7a76\u8005\u304c\u81ea\u4fe1\u3092\u6301\u3063\u3066\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u78ba\u8a8d\u3067\u304d\u308b\u30c4\u30fc\u30eb\u3078\u306e\u8a8d\u8b58\u304c\u9ad8\u307e\u308b\u3053\u3068\u3082\u91cd\u8981\u3067\u3059\u3002\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u306f\u3044\u304f\u3064\u304b\u306e\u5229\u70b9\u304c\u3042\u308a\u3001\u9ad8\u3044\u5206\u96e2\u80fd\u306b\u3088\u3063\u3066\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u5909\u5316\u3092\u691c\u8a3c\u3067\u304d\u308b\u3001\u4fe1\u983c\u3067\u304d\u308b\u65b9\u6cd5\u306e1\u3064\u3067\u3059\u3002\u3055\u3089\u306b\u3001SeqScreener Gene Edit Confirmation App\u3092\u4f7f\u7528\u3059\u308c\u3070\u3001\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u5f97\u3089\u308c\u305f\u30c7\u30fc\u30bf\u306e\u89e3\u91c8\u306f\u3001\u3082\u306f\u3084\u56f0\u96e3\u306a\u4f5c\u696d\u3067\u306f\u306a\u304f\u306a\u308b\u306e\u3067\u3059\u3002<\/p>\n

References<\/strong>
\n1.Cong, L., et al., Multiplex genome engineering using CRISPR\/Cas systems. Science, 2013. 339(6121): p. 819-23.
\n2.Bengtsson, N.E., et al., Muscle-specific CRISPR\/Cas9 dystrophin gene editing ameliorates pathophysiology in a mouse model for Duchenne muscular dystrophy. Nat Commun, 2017. 8: p. 14454.
\n3.Mancuso, P., et al., CRISPR based editing of SIV proviral DNA in ART treated non-human primates. Nat Commun, 2020. 11(1): p. 6065.
\n4.Abbott, T.R., et al., Development of CRISPR as an Antiviral Strategy to Combat SARS-CoV-2 and Influenza. Cell, 2020. 181(4): p. 865-876 e12.
\n5.Vouillot, L., A. Thelie, and N. Pollet, Comparison of T7E1 and surveyor mismatch cleavage assays to detect mutations triggered by engineered nucleases. G3 (Bethesda), 2015. 5(3): p. 407-15.<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u30fbCRISPR\u306f\u30ac\u30a4\u30c9RNA\u3092\u5207\u308a\u66ff\u3048\u308b\u3060\u3051\u3067\u3001\u3055\u307e\u3056\u307e\u306a\u907a\u4f1d\u5b50\u7de8\u96c6\u3078\u306e\u5fdc\u7528\u304c\u53ef\u80fd\u3002
\n\u30fb\u30b2\u30ce\u30e0\u7de8\u96c6\u306f\u3001\u7de8\u96c6\u52b9\u7387\u306e\u78ba\u8a8d\u304c\u975e\u5e38\u306b\u91cd\u8981
\n\u30fbSeqScreener Gene Edit Confirmation App\u3092\u7528\u3044\u308b\u3053\u3068\u306b\u3088\u308a\u3001\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u6ce2\u5f62\u304b\u3089\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u306e\u691c\u8a3c\u304c\u5bb9\u6613\u306b\u5b9f\u65bd\u53ef\u80fd<\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

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