{"id":11467,"date":"2022-05-17T12:34:10","date_gmt":"2022-05-17T03:34:10","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=11467"},"modified":"2025-03-10T11:39:34","modified_gmt":"2025-03-10T02:39:34","slug":"superscript-comparison-bid-ts","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/superscript-comparison-bid-ts\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u9006\u8ee2\u5199\u9175\u7d20SuperScript\u306eII\u3068III\u3068IV\u306f\u9055\u3046\u306e\u304b\uff1f\u5b9f\u969b\u306b\u9006\u8ee2\u5199\u3057\u3066\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7d50\u679c\u3092\u6bd4\u8f03\u3057\u3066\u307f\u305f"},"content":{"rendered":"

\u9006\u8ee2\u5199\u3068\u306f\u3001RNA\u3092cDNA\u306b\u5909\u63db\u3059\u308b\u3053\u3068\u3067\u3042\u308a\u3001RNA\u3092\u30b5\u30f3\u30d7\u30eb\u3068\u3059\u308b\u5206\u5b50\u751f\u7269\u5b66\u5b9f\u9a13\u3067\u306f\u975e\u5e38\u306b\u3088\u304f\u5b9f\u65bd\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306e\u4f8b<\/a>\u3068\u3057\u3066\u306f\u3001\u9006\u8ee2\u5199PCR\uff08RT-PCR\uff09\u3001\u9006\u8ee2\u5199\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08RT-qPCR\uff09\u3001\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3001\u30de\u30a4\u30af\u30ed\u30a2\u30ec\u30a4\u3001RNA-seq\u306a\u3069\u3068\u591a\u5c90\u306b\u4e98\u308a\u307e\u3059\u3002\u305d\u3082\u305d\u3082\u9006\u8ee2\u5199\u9175\u7d20\u306f\u3001\u30ec\u30c8\u30ed\u30a6\u30a4\u30eb\u30b9\u306e\u5fc5\u9808\u5897\u6b96\u56e0\u5b50\u3068\u3057\u3066\u767a\u898b\u3055\u308c\u307e\u3057\u305f\u3002\u751f\u547d\u4f53\u306b\u304a\u3051\u308b\u9006\u8ee2\u5199\u9175\u7d20\u306e\u4e00\u822c\u7684\u306a\u5f79\u5272\u306e1\u3064\u3068\u3057\u3066\u3001RNA\u914d\u5217\u3092cDNA\u306b\u5909\u63db\u3057\u3066\u30b2\u30ce\u30e0\u306e\u5225\u306e\u9818\u57df\u306b\u633f\u5165\u3067\u304d\u308b\u3088\u3046\u306b\u3059\u308b\u3053\u3068\u304c\u6319\u3052\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u306f\u3058\u3081\u306b<\/h2>\n

Invitrogen\u2122 SuperScript\u2122 \u9006\u8ee2\u5199\u9175\u7d20\u30b7\u30ea\u30fc\u30ba\u306f\u30011990\u5e74\u4ee3\u304b\u3089\u304a\u5ba2\u3055\u307e\u306b\u3054\u4f7f\u7528\u3044\u305f\u3060\u3044\u3066\u304a\u308a\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u306b\u6539\u826f\u3092\u91cd\u306d\u7d9a\u3051\u3066\u304a\u308a\u3001\u73fe\u5728SuperScript \u306f\u4e0b\u8a18\u306e3\u7a2e\u985e\u3092\u63d0\u4f9b\u3057\u3066\u3044\u307e\u3059\u3002
\nInvitrogen\u2122 SuperScript\u2122 II Reverse Transcriptase\uff08\u4ee5\u964d\u3001SS2\u3068\u6a19\u8a18\uff09<\/a>
\nInvitrogen\u2122 SuperScript\u2122 III Reverse Transcriptase\uff08\u4ee5\u964d\u3001SS3\u3068\u6a19\u8a18\uff09<\/a>
\nInvitrogen\u2122 SuperScript\u2122 IV Reverse Transcriptase\uff08\u4ee5\u964d\u3001SS4\u3068\u6a19\u8a18\uff09<\/a><\/p>\n

SS2\u2192SS3\u2192SS4\u3068\u3044\u3046\u3088\u3046\u306b\u9806\u6b21\u958b\u767a\u3057\u3001\u30a2\u30c3\u30d7\u30b0\u30ec\u30fc\u30c9\u3057\u3066\u304d\u307e\u3057\u305f\u3002\u3067\u306f\u3001\u305d\u308c\u305e\u308c\u306e\u9006\u8ee2\u5199\u9175\u7d20\u306f\u3069\u3053\u304c\u6539\u826f\u3055\u308c\u3066\u304d\u305f\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\uff1f<\/p>\n

SS2\u304b\u3089SS3\u3078\u306e\u6539\u826f<\/a>
\n\u30fb\u9ad8\u6e29\u3067\u306e\u71b1\u5b89\u5b9a\u6027\u3092\u4ed8\u4e0e\u3057\uff0850 \u2103\uff09\u30012\u6b21\u69cb\u9020\u3092\u6301\u3064RNA\u306b\u5bfe\u5fdc<\/p>\n

SS3\u304b\u3089SS4\u3078\u306e\u6539\u826f<\/a>
\n\u30fb\u593e\u96d1\u963b\u5bb3\u7269\u8cea\u3078\u306e\u8010\u6027\u3092\u4ed8\u4e0e\u3057\u3001\u5b89\u5b9a\u3057\u305f\u9006\u8ee2\u5199\u3092\u5b9f\u73fe
\n\u30fb\u3055\u3089\u306a\u308b\u71b1\u5b89\u5b9a\u6027\u3092\u4ed8\u4e0e\u3057\uff0855 \u2103\u307e\u3067\uff09\u3001\u3088\u308a\u9ad8\u5ea6\u306a2\u6b21\u69cb\u9020\u3092\u6301\u3064RNA\u306b\u3082\u5bfe\u5fdc
\n\u30fb\u9006\u8ee2\u5199\u53cd\u5fdc\u3092\u52a0\u901f\u3057\u3001\u308f\u305a\u304b10 min\u3067\u9ad8\u53ce\u91cf\u306ecDNA\u3092\u56de\u53ce\u53ef\u80fd<\/p>\n

SS2\u2192SS3\u2192SS4\u3068\u6a5f\u80fd\u304c\u5411\u4e0a\u3055\u308c\u3066\u304d\u305f\u306e\u3067\u3059\u304c\u3001\u9006\u8ee2\u5199\u306e\u52b9\u7387\u306f\u7570\u306a\u308b\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u306a\u3093\u3068\u306a\u304f\u3067\u3059\u304c\u3001\u6700\u65b0\u306e\u9175\u7d20\u3067\u3042\u308bSS4\u306f\u3001\u9006\u8ee2\u5199\u52b9\u7387\u3082\u9ad8\u305d\u3046\u306a\u30a4\u30e1\u30fc\u30b8\u3067\u3059\u3002\u305d\u3053\u3067\u4eca\u56de\u306f\u3001SS2\u3001SS3\u3001SS4\u3092\u4f7f\u7528\u3057\u3066\u540c\u3058\u6761\u4ef6\u3067\u9006\u8ee2\u5199\u3057\u3001\u305d\u306ecDNA\u3092\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306b\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u5b9f\u65bd\u3059\u308b\u3053\u3068\u3067SuperScript \u9006\u8ee2\u5199\u9175\u7d20\u30b7\u30ea\u30fc\u30ba\u3092\u6bd4\u8f03\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n

\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n

\u6750\u6599\uff1a
\nHeLa\u7d30\u80de\u304b\u3089Invitrogen\u2122 PureLink\u2122 RNA Mini Kit<\/a>\u3092\u7528\u3044\u3066Total RNA\u3092\u62bd\u51fa\u3057\u307e\u3057\u305f\u3002\u62bd\u51fa\u3057\u305fRNA\u306e\u6fc3\u5ea6\u30fb\u54c1\u8cea\u306f\u3001Thermo Scientific\u2122 NanoDrop\u2122 OneC<\/a>\u3092\u7528\u3044\u3066\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002\u6fc3\u5ea6\u306f252.6 ng\/\u00b5L\u3001A260\/A280\u306f2.05\u3001A260\/A230\u306f2.21\u3067\u3057\u305f\u3002\u307e\u305f\u3001Agilent\u2122 2100 \u30d0\u30a4\u30aa\u30a2\u30ca\u30e9\u30a4\u30b6\uff08RNA6000\u30d4\u30b3\u30ad\u30c3\u30c8\uff09\u3067\u7b97\u51fa\u3057\u305fRIN\u5024\u306f9.70\u3067\u3057\u305f\u3002<\/p>\n

\u9006\u8ee2\u5199\u9175\u7d20\u306f\u4ee5\u4e0b\u306e3\u7a2e\u985e\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002
\nInvitrogen\u2122 SuperScript\u2122 II Reverse Transcriptase\uff08\u88fd\u54c1\u756a\u53f7\uff1a18064022\uff09<\/a>
\nInvitrogen\u2122 SuperScript\u2122 III Reverse Transcriptase\uff08\u88fd\u54c1\u756a\u53f7\uff1a18080093\uff09<\/a>
\nInvitrogen\u2122 SuperScript\u2122 IV Reverse Transcriptase\uff08\u88fd\u54c1\u756a\u53f7\uff1a18090010\uff09<\/a><\/p>\n

\u65b9\u6cd5\uff1a
\n\u9006\u8ee2\u5199\u53cd\u5fdc\u306f\u9175\u7d20\u306b\u3088\u3063\u3066\u82e5\u5e72\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u304c\u7570\u306a\u3063\u3066\u3044\u307e\u3059\uff08\u88681\uff09\u3002<\/p>\n

\"\"<\/p>\n

\u4eca\u56de\u306f\u3001\u4e0a\u8a18SS2\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3067\u3042\u308b42 \u2103\u306750 min\u306e\u6761\u4ef6\u3068\u3001SS4\u306e55 \u2103\u306710 min\u3068\u3044\u3046\u6761\u4ef6\u3067\u9006\u8ee2\u5199\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002SS2\u3068SS4\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u3067\u306f\u3001\u9006\u8ee2\u5199\u6e29\u5ea6\u3068\u6642\u9593\u306e\u4ed6\u306b\u3001DTT\u306e\u7d42\u6fc3\u5ea6\u304c\u7570\u306a\u3063\u3066\u3044\u307e\u3059\u30025\u00d7Buffer\u306f\u305d\u308c\u305e\u308c\u306e\u9006\u8ee2\u5199\u9175\u7d20\u306b\u6dfb\u4ed8\u306e\u3082\u306e\u3092\u4f7f\u7528\u3057\u3001\u305d\u308c\u4ee5\u5916\u306eOligo d(T)20 <\/sub>primer\u3001Total RNA\u3001dNTP\u3001Nuclease Free Water\u3001DTT\u3001RNaseOUT RNase Inhibitor\u306f\u5225\u9014\u6e96\u5099\u3057\u3066\u5171\u901a\u306e\u3082\u306e\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u3002RNA\u306e\u4f7f\u7528\u91cf\u306fSS2\u306e\u4e0b\u9650\u3067\u3042\u308b1 ng\/20 \u00b5L\u3068\u3001100 ng\/20 \u00b5L\u306e2\u30d1\u30bf\u30fc\u30f3\u3067\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u9006\u8ee2\u5199\u3057\u305fcDNA\u306f\u3001\u6b21\u306e\u5b9f\u9a13\u306b\u4f7f\u7528\u3059\u308b\u307e\u3067-20\u2103\u3067\u4fdd\u5b58\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u9006\u8ee2\u5199\u9175\u7d20\u306e\u9055\u3044\u306b\u3088\u308b\u5f71\u97ff\u306f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3067\u8a55\u4fa1\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n\n\n\n\n
\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u53cd\u5fdc\u6db2<\/strong><\/td>\n(\u03bcL)<\/strong><\/td>\n<\/tr>\n
Applied Biosystems\u2122 TaqMan\u2122 Fast Advanced Master Mix<\/a><\/td>\n5<\/td>\n<\/tr>\n
Applied Biosystems\u2122 TaqMan Gene Expression Assay<\/a> (20x) GAPDH<\/td>\n0.5<\/td>\n<\/tr>\n
Nuclease-Free Water<\/a><\/td>\n2.5<\/td>\n<\/td>\n<\/tr>\n
Subtotal<\/td>\n8<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b\u30a6\u30a7\u30eb\u9593\u3067\u8abf\u88fd\u8aa4\u5dee\u304c\u751f\u3058\u306a\u3044\u3088\u3046\u306b\u3059\u308b\u305f\u3081\u3001\u5fc5\u8981\u672c\u6570\u5206\u307e\u3068\u3081\u3066\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002<\/p>\n\n\n\n\n
cDNA\uff0810 ng\/well \u3082\u3057\u304f\u306f 0.1 ng\/well\uff09<\/td>\n2<\/td>\n<\/tr>\n
Total<\/td>\n10<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u3053\u3053\u3067RNA\u3068cDNA\u306e\u6fc3\u5ea6\u3092\u78ba\u8a8d\u3057\u3066\u304a\u304d\u305f\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u9006\u8ee2\u5199\u5f8c\u306ecDNA\u6eb6\u6db2\u306b\u306f\u3001\u9006\u8ee2\u5199\u9175\u7d20\u3084RNA\u6b8b\u6e23\u3001dNTP\u306a\u3069\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u306e\u3067\u3001\u6b63\u78ba\u306a\u6fc3\u5ea6\u6e2c\u5b9a\u304c\u56f0\u96e3\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u4f7f\u7528\u3057\u305fRNA\u304c100% cDNA\u306b\u5909\u63db\u3055\u308c\u305f\u3068\u4eee\u5b9a\u3057\u3066\u3001\u7b49\u4fa1\u63db\u7b97\u3067cDNA\u6fc3\u5ea6\u3092\u7b97\u51fa\u3059\u308b\u306e\u304c\u4e00\u822c\u7684\u3067\u3059\u3002
\nRNA 100 ng\/20 \u00b5L\u2192cDNA 100 ng\/20 \u00b5L\uff085 ng\/\u00b5L\uff09
\nRNA 1 ng\/20 \u00b5L\u2192cDNA 1 ng\/20 \u00b5L\uff080.05 ng\/\u00b5L\uff09
\n\u3053\u308c\u3089\u305d\u308c\u305e\u308c\u30922 \u00b5L\/well\u3067\u4f7f\u7528\u3059\u308b\u306e\u3067\u300110 ng\/well\u3082\u3057\u304f\u306f0.1 ng\/well\u306ecDNA\u3092\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u3068\u3057\u3066\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0\u306f\u3001Applied Biosystems\u2122 QuantStudio\u2122 5 Real-Time PCR System, 384-well<\/a>\u3092\u4f7f\u7528\u3057\u3001Fast \u30e2\u30fc\u30c9\u306b\u8a2d\u5b9a\u3057\u3066\u4e0b\u8a18\u6761\u4ef6\u3067\u30e9\u30f3\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\uff08n = 12\uff09\u3002<\/p>\n

1. 95 \u2103 20 sec
\n2. 95 \u2103 1 sec
\n3. 60 \u2103 20 sec
\n\u203b2\u30683\u309240 cycle \u53cd\u5fa9<\/p>\n

\u7d50\u679c\u3068\u8003\u5bdf<\/h2>\n

\u305d\u308c\u3067\u306f\u3001\u7d50\u679c\u3092\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

SS2\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306b\u5f93\u304442 \u2103\u306750 min\u9006\u8ee2\u5199\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u5897\u5e45\u66f2\u7dda\u3092\u78ba\u8a8d\u3059\u308b\u3068\u300110 ng\/well\u30680.1 ng\/well\u306e2\u3064\u306e\u5897\u5e45\u66f2\u7dda\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\uff08\u56f31\uff09\u3002\u305d\u308c\u305e\u308c\u306e\u6761\u4ef6\u306e\u30c6\u30af\u30cb\u30ab\u30eb\u30ec\u30d7\u30ea\u30b1\u30fc\u30c8\u306f12\u53cd\u5fa9\u3067\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002cDNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u91cf\u304c\u591a\u304410 ng\/well\u306f\u5de6\u5074\u306e36\u672c\u306e\u66f2\u7dda\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u91cf\u304c\u5c11\u306a\u30440.1 ng\/well\u306f\u53f3\u5074\u306e36\u672c\u306e\u66f2\u7dda\u3067\u3059\u3002\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u91cf\u306e\u9055\u3044\u306f\u306f\u3063\u304d\u308a\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u9006\u8ee2\u5199\u9175\u7d20\u306e\u9055\u3044\u306f\u5897\u5e45\u66f2\u7dda\u304b\u3089\u306f\u8aad\u307f\u53d6\u308c\u307e\u305b\u3093\u3002
\n\u6b21\u306b\u3001SS4\u306e\u30d7\u30ed\u30c8\u30b3\u30fc\u30eb\u306b\u5f93\u304455 \u2103\u306710 min\u9006\u8ee2\u5199\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306e\u5897\u5e45\u66f2\u7dda\u3092\u78ba\u8a8d\u3059\u308b\u3068\u30013\u3064\u306e\u5897\u5e45\u66f2\u7dda\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\uff08\u56f32\uff09\u3002\u3053\u3061\u3089\u3082\u30c6\u30af\u30cb\u30ab\u30eb\u30ec\u30d7\u30ea\u30b1\u30fc\u30c8\u306f12\u53cd\u5fa9\u3067\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002\u4e00\u756a\u53f3\u306e\u30d4\u30f3\u30af\u306e\u5897\u5e45\u66f2\u7dda\u306f\u30011 ng\u3068\u3044\u3046\u6bd4\u8f03\u7684\u5c11\u91cf\u306eRNA\u3092SS2\u306b\u306655 \u2103\u306710 min\u9006\u8ee2\u5199\u3057\u305fcDNA\u3092\u7528\u3044\u305f\u7d50\u679c\u3067\u3059\u3002SS2\u306e\u901a\u5e38\u306e\u9006\u8ee2\u5199\u6761\u4ef6\u306f42\u2103\u300150 min\u3067\u3059\u306e\u3067\uff08\u88681\uff09\u300155 \u2103\u300110 min\u306f\u9ad8\u6e29\u3067\u77ed\u6642\u9593\u3068\u3044\u3046\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u307e\u305fSS2\u306f\u3001SS3\u3084SS4\u3068\u6bd4\u8f03\u3057\u3066\u71b1\u5b89\u5b9a\u6027\u304c\u4f4e\u3044\u3067\u3059\uff08SuperScript II \u3068 III \u306e\u6bd4\u8f03<\/a>\uff09\u3002\u3053\u306e\u305f\u3081\u9006\u8ee2\u5199\u52b9\u7387\u304c\u4f4e\u4e0b\u3057\u3066\u3057\u307e\u3044\u3001\u5897\u5e45\u66f2\u7dda\u306eCT<\/sub>\u5024\u304c\u9ad8\u304f\u306a\u3063\u305f\uff08\u5897\u5e45\u66f2\u7dda\u304c\u53f3\u306b\u30b7\u30d5\u30c8\uff09\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n

\"\"<\/p>\n

\"\"<\/p>\n

\u5b9a\u91cf\u7d50\u679c\u3092\u3082\u3046\u5c11\u3057\u8a73\u3057\u304f\u6bd4\u8f03\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002
\n\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u5b9a\u91cf\u5024\u3067\u3042\u308bCT<\/sub>\u5024\u3092\u56f3\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u300242 \u2103\u306750 min\u9006\u8ee2\u5199\u3057\u305f\u7d50\u679c\u304c\u56f33\u5de6\u300155 \u2103\u306710 min\u9006\u8ee2\u5199\u3057\u305f\u7d50\u679c\u304c\u56f33\u53f3\u3067\u3059\u3002\u307e\u305f\u3001\u56f33\u306eCT<\/sub>\u5024\u306e\u6570\u5024\u3092\u88682\u306b\u307e\u3068\u3081\u307e\u3057\u305f\u3002
\n\u5148\u307b\u3069\u5897\u5e45\u66f2\u7dda\u3067\u78ba\u8a8d\u3057\u305f\u901a\u308a\u300142 \u2103\u306750 min\u3067\u9006\u8ee2\u5199\u3057\u305f\u7d50\u679c\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8cDNA\u306e\u4f7f\u7528\u91cf\u306b\u8d77\u56e0\u3059\u308b\u5dee\u306f\u898b\u3089\u308c\u307e\u3059\u304c\u3001\u9006\u8ee2\u5199\u9175\u7d20\u306e\u9055\u3044\u306b\u3088\u308b\u5dee\u306f\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\uff08\u56f33\u5de6\uff09\u3002\u307e\u305f\u300155 \u2103\u306710 min \u9006\u8ee2\u5199\u3057\u305f\u7d50\u679c\u3001\u5c11\u91cf\u306eRNA\u3092SS2\u3067\u9006\u8ee2\u5199\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306eCT<\/sub>\u5024\u304cSS3\u3068SS4\u3068\u6bd4\u8f03\u3057\u3066\u76ee\u7acb\u3063\u3066\u9ad8\u304f\u306a\u3063\u3066\u3044\u307e\u3057\u305f\uff08\u56f33\u53f3\uff09\u3002CT<\/sub>\u5024\u306e\u5e73\u5747\u306f\u3001SS2\u304c28.1\u3001SS4\u304c25.4\u306a\u306e\u3067\u3001\u305d\u306e\u5dee\u306f2.7\u30b5\u30a4\u30af\u30eb\u3067\u3057\u305f\u3002PCR\u306e\u539f\u7406\u304b\u30891\u30b5\u30a4\u30af\u30eb\u30672\u500d\u306e\u5dee\u306a\u306e\u3067\u3001\u7d046.7\u500d\u306e\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u91cf\u306e\u5dee\u3067\u3042\u308b\u3068\u8a08\u7b97\u3067\u304d\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306aSS2\u306e\u9006\u8ee2\u5199\u52b9\u7387\u306e\u4f4e\u4e0b\u306f\u3001\u672c\u6765\u306e\u9006\u8ee2\u5199\u6e29\u5ea6\u3067\u3042\u308b42 \u2103\u304b\u308913 \u2103\u3082\u9ad8\u3044\u300155 \u2103\u3067\u9006\u8ee2\u5199\u3057\u305f\u305f\u3081\u306b\u9006\u8ee2\u5199\u9175\u7d20\u6d3b\u6027\u304c\u4f4e\u4e0b\u3057\u305f\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u3088\u308a\u591a\u3044\u91cf\u306eRNA\u3092\u9006\u8ee2\u5199\u3057\u305f\u7d50\u679c\u3067\u306f\u3001SS2\u306e\u9ad8\u6e29\u306b\u304a\u3051\u308b\u9006\u8ee2\u5199\u6d3b\u6027\u306e\u4f4e\u4e0b\u306f\u9855\u8457\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\uff08\u56f33\u53f3\uff09\u3002\u3057\u304b\u3057\u3001CT<\/sub>\u5024\u306e\u5e73\u5747\u5024\u3068\u3057\u3066\u306f\u3001SS2\u306f19.0\u3001SS3\u306f18.8\u3001SS4\u306f18.5\u3060\u3063\u305f\u306e\u3067\u3001\u591a\u5c11\u306f\u9ad8\u6e29\u306b\u3088\u308b\u5f71\u97ff\u304c\u8868\u308c\u305f\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002<\/p>\n

\"\" \"\"<\/p>\n

 <\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u3044\u304b\u304c\u3067\u3057\u305f\u3067\u3057\u3087\u3046\u304b\u3002
\n\u4eca\u56de\u306f SuperScript \u9006\u8ee2\u5199\u9175\u7d20\u3092\u6bd4\u8f03\u3057\u3066\u307f\u307e\u3057\u305f\u3002\u611f\u60f3\u3068\u3057\u3066\u306f\u3001SS2\u3068SS3\u3068SS4\u3067\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7d50\u679c\u306b\u601d\u3044\u306e\u307b\u304b\u5927\u304d\u306a\u5dee\u306f\u51fa\u306a\u3044\u306e\u3060\u306a\u3068\u601d\u3044\u307e\u3057\u305f\u3002\u7570\u306a\u308bSuperScript \u9006\u8ee2\u5199\u9175\u7d20\u3092\u4f7f\u7528\u3057\u3066\u3082\u5b89\u5b9a\u3057\u305f\u5b9a\u91cf\u5024\u3092\u5f97\u3089\u308c\u308b1\u3064\u306e\u826f\u3044\u4f8b\u306b\u306a\u3063\u305f\u304b\u3068\u601d\u3044\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u4eca\u56de\u306e\u5b9f\u9a13\u6761\u4ef6\u3067\u306f\u3001RNA\u306e2\u6b21\u69cb\u9020\u3084\u593e\u96d1\u7269\u306e\u6df7\u5165\u306e\u5f71\u97ff\u3001\u9006\u8ee2\u5199\u53ef\u80fd\u306aRNA\u306e\u9577\u3055\u306a\u3069\u306b\u3064\u3044\u3066\u306f\u691c\u8a0e\u3057\u307e\u305b\u3093\u3067\u3057\u305f\u3002SS4\u3067\u306f\u308f\u305a\u304b10 min\u3068\u3044\u3046\u77ed\u6642\u9593\u306e\u9006\u8ee2\u5199\u3067\u30829 kb\u307e\u3067\u306eRNA\u3092\u9006\u8ee2\u5199\u53ef\u80fd\u3067\u3059\u304c\u3001SS3\u3067\u306f\u77ed\u6642\u9593\u3067\u306f\u305d\u3053\u307e\u3067\u306e\u9577\u9396\u3092\u9006\u8ee2\u5199\u3067\u304d\u307e\u305b\u3093\uff08SuperScript III \u3068 IV \u306e\u6bd4\u8f03<\/a>\u306e\u30da\u30fc\u30b8\u306e\u56f34\uff09\u3002<\/p>\n

SS4\u306f\u3001RNA\u306e\u9ad8\u5ea6\u306a2\u6b21\u69cb\u9020\u306b\u3082\u5bfe\u5fdc\u3067\u304d\u308b\u9ad8\u6e29\u5b89\u5b9a\u6027\u3092\u6709\u3057\u3001\u593e\u96d1\u963b\u5bb3\u7269\u8cea\u3078\u306e\u8010\u6027\u304c\u5927\u5e45\u306b\u5411\u4e0a\u3057\u3066\u304a\u308a\u3001\u8ee2\u5199\u53cd\u5fdc\u6642\u9593\u309250 min\u304b\u308910 min\u306b\u77ed\u7e2e\u3067\u304d\u308b\u3001\u9ad8\u6a5f\u80fd\u306a\u9006\u8ee2\u5199\u9175\u7d20\u3067\u3059\u3002\u3053\u308c\u3092\u6a5f\u4f1a\u306b\u3001\u6700\u65b0\u306eSuperScript \u9006\u8ee2\u5199\u9175\u7d20\u3067\u3042\u308bSS4\u3078\u306e\u30a2\u30c3\u30d7\u30b0\u30ec\u30fc\u30c9\u306f\u3044\u304b\u304c\u3067\u3057\u3087\u3046\u304b\uff1fSuperScript \u9006\u8ee2\u5199\u9175\u7d20\u3092\u304a\u4f7f\u3044\u3044\u305f\u3060\u304f\u969b\u306b\u306f\u3001\u5fc5\u305a\u304a\u4f7f\u3044\u306e\u9175\u7d20\u306e\u53d6\u6271\u8aac\u660e\u66f8\u306b\u5f93\u3063\u3066\u5b9f\u9a13\u3092\u9032\u3081\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u5f53\u793e\u3067\u306fRNA\u62bd\u51fa\u3084\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3001\u4ed6\u306b\u3082\u7d30\u80de\u57f9\u990a\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306a\u3069\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\uff08\u5b9f\u7fd2\uff09\u3092\u884c\u3044\u3064\u3064\u5b66\u3079\u308b\u5404\u7a2e\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/a>\u3092\u958b\u50ac\u3057\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u4e2d\u3067\u4eca\u56de\u306e\u3088\u3046\u306a\u5b9f\u9a13\u7d50\u679c\u3082\u3054\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u65b0\u3057\u3044\u5b9f\u9a13\u3092\u59cb\u3081\u3089\u308c\u308b\u65b9\u3001\u3088\u308a\u7406\u89e3\u3092\u6df1\u3081\u305f\u3044\u65b9\u306f\u305c\u3072\u3054\u53c2\u52a0\u304f\u3060\u3055\u3044\uff01<\/p>\n

\u5206\u5b50\u751f\u7269\u5b66\u6559\u5ba4PDF\u7248 \u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9<\/h3>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u306f\u3053\u3061\u3089<\/a><\/p>\n

\u4eca\u56de\u306e\u8a18\u4e8b\u306b\u95a2\u9023\u3059\u308b\u904e\u53bb\u306e\u8a18\u4e8b\uff1a
\n\u9006\u8ee2\u5199\u6559\u5ba4\uff1a\u6982\u8aac<\/a>
\n\u9006\u8ee2\u5199\u6559\u5ba4\uff1a\u6700\u3082\u4e00\u822c\u7684\u306a6\u3064\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3<\/a>
\n\u9006\u8ee2\u5199\u53cd\u5fdc\u306e\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u30ac\u30a4\u30c9<\/a>
\nSuperScript IV \u9006\u8ee2\u5199\u9175\u7d20<\/a>
\nSuperScript II \u3068 III \u306e\u6bd4\u8f03<\/a>
\nSuperScript III \u3068 IV \u306e\u6bd4\u8f03<\/a><\/p>\n

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