{"id":1179,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_44_3_attune\/"},"modified":"2025-02-28T10:40:16","modified_gmt":"2025-02-28T01:40:16","slug":"user_voice_44_3_attune","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_44_3_attune\/","title":{"rendered":"A trip to the small world of fetal lymph node\uff08\u5317\u6d77\u9053\u5927\u5b66\u907a\u4f1d\u5b50\u75c5\u5236\u5fa1\u7814\u7a76\u6240 \u6fa4\u65b0\u4e00\u90ce\u6c0f\uff09-\u300cNEXT\u300d2018\u5e743\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2><span style=\"font-size: 12pt\">A trip to the small world of fetal lymph node<\/span><\/h2>\n<p><strong>\u6fa4\u65b0\u4e00\u90ce \u6c0f \uff08\u5317\u6d77\u9053\u5927\u5b66\u907a\u4f1d\u5b50\u75c5\u5236\u5fa1\u7814\u7a76\u6240\u611f\u67d3\u75c5\u614b\u5206\u91ce \u51c6\u6559\u6388\uff09<\/strong><br \/>\n\u203b\u5f53\u8a18\u4e8b\u306f\u7b2c46\u56de\u65e5\u672c\u514d\u75ab\u5b66\u4f1a\u5b66\u8853\u96c6\u4f1a\u3000\u30c6\u30af\u30cb\u30ab\u30eb\u30bb\u30df\u30ca\u30fc \uff082017\u5e7412\u670813\u65e5 \u958b\u50ac\uff09\u306e\u767a\u8868\u5185\u5bb9\u3067\u3059<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-12.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-4521\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-12.jpg\" alt=\"\u6fa4\u65b0\u4e00\u90ce \u6c0f\uff08\u5317\u6d77\u9053\u5927\u5b66\u907a\u4f1d\u5b50\u75c5\u5236\u5fa1\u7814\u7a76\u6240\u611f\u67d3\u75c5\u614b\u5206\u91ce \u51c6\u6559\u6388\uff09\" width=\"250\" height=\"250\" \/><\/a>\u300c\u3053\u306e\u30bb\u30df\u30ca\u30fc\u3067\u306f\u3001\u300e\u514d\u75ab\u306e\u5834\u300f\u3068\u3057\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u62c5\u3046\u30ea\u30f3\u30d1\u7bc0\u304c\u3069\u3046\u3084\u3063\u3066\u3067\u304d\u308b\u306e\u304b\u3001\u30ea\u30f3\u30d1\u7bc0\u767a\u751f\u306b\u95a2\u308f\u308b\u7279\u6b8a\u306a\u7d30\u80de\u305f\u3061\u306e\u6319\u52d5\u3068\u305d\u306e\u5206\u5b50\u6a5f\u69cb\u3092\u7d39\u4ecb\u3057\u307e\u3059\u3002\u307e\u305f\u6700\u5f8c\u306b\u3001\u3053\u306e\u5c0f\u3055\u306a\u7d30\u80de\u306e\u4e16\u754c\u3092\u89e3\u6790\u3059\u308b\u306b\u3042\u305f\u308a\u3001\u30de\u30a4\u30ca\u30fc\u30dd\u30d4\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u7d30\u80de\u89e3\u6790\u306b\u304a\u3051\u308bAttune NxT\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30fc\u30bf\u30fc\u306e\u6d3b\u7528\u306b\u3064\u3044\u3066\u8a71\u3057\u307e\u3059\u300d\u3068\u5317\u6d77\u9053\u5927\u5b66\u306e\u6fa4\u65b0\u4e00\u90ce\u6c0f\u306f\u30bb\u30df\u30ca\u30fc\u3092\u958b\u59cb\u3057\u307e\u3057\u305f\u3002\u611f\u67d3\u9632\u5fa1\u306e\u6700\u524d\u7dda\u3067\u3042\u308a\u3001\u6297\u539f\u6297\u4f53\u53cd\u5fdc\u304c\u8d77\u304d\u308b\u300c\u5834\u300d\u3068\u3057\u3066\u79c1\u305f\u3061\u306e\u4f53\u4e2d\u306b\u6563\u5728\u3059\u308b\u6570\u767e\u306e\u30ea\u30f3\u30d1\u7bc0\u3002\u30d7\u30ed\u30b0\u30e9\u30e0\u3055\u308c\u305f\u30ea\u30f3\u30d1\u7bc0\u306e\u767a\u751f\u6a5f\u5e8f\u306e\u89e3\u660e\u3092\u76ee\u6307\u3059\u6fa4\u6c0f\u306e\u30bb\u30df\u30ca\u30fc\u3092\u5831\u544a\u3057\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u30ea\u30f3\u30d1\u7bc0\u767a\u751f\u306f2\u7a2e\u985e\u306e\u7d30\u80de\u306e\u51fa\u4f1a\u3044\u304b\u3089<\/span><\/h2>\n<p>\u30ea\u30f3\u30d1\u7bc0\u306f\u3001\u5468\u8fba\u7d44\u7e54\u304b\u3089\u904b\u3073\u8fbc\u307e\u308c\u305f\u6297\u539f\u3092\u30ea\u30f3\u30d1\u7403\u306b\u63d0\u793a\u3057\u305f\u308a\u3001\u52b9\u679c\u7684\u306a\u514d\u75ab\u5fdc\u7b54\u3092\u8a98\u5c0e\u3057\u305f\u308a\u3001\u8a18\u61b6\u514d\u75ab\u3092\u6210\u7acb\u3055\u305b\u305f\u308a\u3059\u308b\u305f\u3081\u306b\u91cd\u8981\u306a\u300c\u514d\u75ab\u306e\u5834\u300d\u3068\u306a\u308a\u307e\u3059\u3002\u6210\u719f\u3057\u305f\u30ea\u30f3\u30d1\u7bc0\u306fB\u7d30\u80de\u3084T\u7d30\u80de\u304c\u5bcc\u3080\u9818\u57df\u304c\u3042\u308a\u3001\u305d\u306e\u5f62\u6210\u3084\u7dad\u6301\u306b\u306f\u9818\u57df\u3054\u3068\u306b\u7279\u6b8a\u306a\u9593\u8449\u7cfb\uff08\u30b9\u30c8\u30ed\u30fc\u30de\uff09\u7d30\u80de\u304c\u95a2\u4e0e\u3057\u3066\u3044\u307e\u3059\u3002\u30de\u30a6\u30b9\u306b\u304a\u3051\u308b\u30ea\u30f3\u30d1\u7bc0\u306e\u5f62\u6210\u306f\u3001\u80ce\u751f15\u65e5\u306b\u4f53\u4e2d\u3092\u5faa\u74b0\u3059\u308b\u8840\u6db2\u7cfb\u7d30\u80de\u306e\u4e00\u7a2e\u3067\u3042\u308bLymphoid Tissue inducer (LTi\uff09\u7d30\u80de\u304c\u3001\u30ea\u30f3\u30d1\u7bc0\u306e\u57fa\u306b\u306a\u308b\u9818\u57df\uff08\u30ea\u30f3\u30d1\u7d44\u7e54\u539f\u57fa\uff09\u306b\u304a\u3044\u3066Lymphoid Tissue organizer (LTo)\u7d30\u80de\u3068\u547c\u3070\u308c\u308b\u30b9\u30c8\u30ed\u30fc\u30de\u7d30\u80de\u3068\u300c\u51fa\u4f1a\u3046\u300d\u3053\u3068\u3067\u958b\u59cb\u3055\u308c\u307e\u3059\u3002\u307e\u305f\u30ea\u30f3\u30d1\u7bc0\u306e\u767a\u751f\u306b\u306f\u30012\u985e\u306e\u30b1\u30e2\u30ab\u30a4\u30f3\u3067\u3042\u308bRANKL*1\u3068LT\u03b11\u03b22*2\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002\u79c1\u305f\u3061\u306fRANKL\u3068\u305d\u306e\u53d7\u5bb9\u4f53\u3067\u3042\u308bRANK\u3092\u7d30\u80de\u7279\u7570\u7684\u306b\u6b20\u640d\u3059\u308b\u30de\u30a6\u30b9\u306e\u89e3\u6790\u304b\u3089\u3001\u30ea\u30f3\u30d1\u7bc0\u306e\u767a\u751f\u306b\u306fLTi\u7d30\u80de\u306b\u304a\u3051\u308bRANK\u3068LTo\u7d30\u80de\u306b\u304a\u3051\u308bRANKL\u304c\u5fc5\u8981\u3067\u3042\u308b\u3053\u3068\u3001\u3055\u3089\u306bLTi\u7d30\u80de\u306bRANKL\u304b\u3089\u306e\u523a\u6fc0\u304c\u5165\u308b\u3053\u3068\u3067\u30ea\u30f3\u30d1\u7bc0\u5f62\u6210\u306e\u305f\u3081\u306e\u6700\u7d42\u5206\u5316\u3078\u9032\u3080\u3053\u3068\u3092\u7a81\u304d\u6b62\u3081\u307e\u3057\u305f\u3002<br \/>\n<span style=\"font-size: 8pt\">*1 RANKL: Receptor Activator of NF-B Ligand<\/span><br \/>\n<span style=\"font-size: 8pt\">*2 LT\u03b11\u03b22\u708e\u75c7\u6027\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u306e\u4e00\u3064\u3067\u3042\u308b\u30ea\u30f3\u30d5\u30a9\u30c8\u30ad\u30b7\u30f3<\/span><\/p>\n<h2><span style=\"font-size: 12pt\">\u30ea\u30f3\u30d1\u7bc0\u767a\u751f\u306b\u304a\u3051\u308bRANKL\u306e\u5f79\u5272\u3068\u306f?<\/span><\/h2>\n<p>RANKL\u306fTNF\u30ea\u30ac\u30f3\u30c9\u30d5\u30a1\u30df\u30ea\u30fc\u306b\u5c5e\u3059\u308b\u819c\u7d50\u5408\u578b\u30b5\u30a4\u30c8\u30ab\u30a4\u30f3\u3067\u3042\u308a\u3001LTi\u7d30\u80de\u3068LTo\u7d30\u80de\u306e\u4e21\u65b9\u306b\u767a\u73fe\u3057\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u30ea\u30f3\u30d1\u7bc0\u306e\u307f\u306a\u3089\u305a\u3001\u9aa8\u3084\u80f8\u817a\u306e\u767a\u751f\u306b\u304a\u3044\u3066\u3082\u91cd\u8981\u306a\u50cd\u304d\u3092\u62c5\u3063\u3066\u3044\u307e\u3059\u3002\u305d\u3053\u3067\u591a\u69d8\u306a\u6a5f\u80fd\u3092\u6709\u3059\u308bRANKL\u306e\u30ea\u30f3\u30d1\u7bc0\u5f62\u6210\u306b\u304a\u3051\u308b\u50cd\u304d\u3092\u53d7\u5bb9\u4f53RANK\u3068\u306e\u95a2\u4fc2\u304b\u3089\u8abf\u3079\u308b\u3053\u3068\u306b\u3057\u307e\u3057\u305f\u3002\u307e\u305aRANK\u30ec\u30dd\u30fc\u30bf\u30fc\u30de\u30a6\u30b9\u3092\u89b3\u5bdf\u3057\u305f\u7d50\u679c\u3001\u30ea\u30f3\u30d1\u7ba1\u306bRANK\u304c\u767a\u73fe\u3059\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u30ea\u30f3\u30d1\u7ba1\u7279\u7570\u7684\u306bRANK\u3092\u6b20\u640d\u3059\u308b\u30de\u30a6\u30b9\u3092\u4f5c\u88fd\u3057\u305f\u3068\u3053\u308d\u3001\u90e8\u5206\u7684\u306b\u30ea\u30f3\u30d1\u7bc0\u5f62\u6210\u304c\u963b\u5bb3\u3055\u308c\u3001LTi\u7d30\u80de\u306e\u751f\u5b58\u7dad\u6301\u306b\u5fc5\u8981\u306a\u30b1\u30e2\u30ab\u30a4\u30f3\u3084\u8840\u7ba1\u65b0\u751f\u56e0\u5b50\u306e\u91cf\u304c\u6e1b\u5c11\u3059\u308b\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u4ee5\u4e0a\u306e\u3053\u3068\u306f\u3001LTo\u7d30\u80de\u4e0a\u306eRANKL\u306f\u3001LTi\u7d30\u80de\u3060\u3051\u3067\u306a\u304f\u30ea\u30f3\u30d1\u7ba1\u306b\u767a\u73fe\u3059\u308bRNAK\u3082\u50cd\u304d\u304b\u3051\u3001LTi\u7d30\u80de\u304c\u5fdc\u7b54\u3059\u308b\u30b1\u30e2\u30ab\u30a4\u30f3\u3084\u8840\u7ba1\u65b0\u751f\u56e0\u5b50\u3092\u5206\u6ccc\u3055\u305b\u308b\u3053\u3068\u3067\u3001\u8840\u6db2\u4e2d\u306eLTi\u7d30\u80de\u3092\u30ea\u30f3\u30d1\u7bc0\u539f\u57fa\u3078\u547c\u3073\u5bc4\u305b\u3001\u84c4\u7a4d\u3055\u305b\u308b\u3053\u3068\u306b\u95a2\u4e0e\u3059\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u30de\u30a4\u30ca\u30fc\u30dd\u30d4\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u306e\u7d30\u80de\u306e\u5c65\u6b74\u3092\u8abf\u3079\u308b<\/span><\/h2>\n<p>\u6700\u5f8c\u306b\u30ea\u30f3\u30d1\u7bc0\u767a\u751f\u306b\u304a\u3044\u3066\u91cd\u8981\u306a\u5f79\u5272\u3092\u62c5\u3046RANKL\u967d\u6027LTo\u7d30\u80de\u3068\u3001\u6210\u719f\u3057\u305f\u30ea\u30f3\u30d1\u7bc0\u306b\u5b58\u5728\u3059\u308b\u9818\u57df\u7279\u7570\u7684\u306a\u30b9\u30c8\u30ed\u30fc\u30de\u7d30\u80de\u306e\u95a2\u4fc2\u6027\u3092\u8abf\u3079\u307e\u3057\u305f\u3002\u6700\u521d\u306b\u7d44\u7e54\u67d3\u8272\u3067DNA\u30ea\u30b3\u30f3\u30d3\u30ca\u30fc\u30bcCre\u3092RANKL\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u4e0b\u6d41\u3067\u767a\u73fe\u3059\u308bRANKL-Cre\u30de\u30a6\u30b9\u306e\u30ea\u30f3\u30d1\u7bc0\u3092\u89b3\u5bdf\u3059\u308b\u3068\u3001\u591a\u304f\u306e\u30b9\u30c8\u30ed\u30fc\u30de\u7d30\u80de\u3067RANKL\u304c\u767a\u73fe\u3057\u305f\u5c65\u6b74\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u8a73\u7d30\u306b\u8abf\u3079\u308b\u305f\u3081\u306b\u30014\u8272\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u907a\u4f1d\u5b50\u3092\u7d44\u307f\u8fbc\u3093\u3060Confetti\u30de\u30a6\u30b9\u3092\u6d3b\u7528\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u30de\u30a6\u30b9\u3092RANKL-Cre\u30de\u30a6\u30b9\u3068\u304b\u3051\u5408\u305b\u308b\u30681\u56de\u306e\u76f8\u540c\u7d44\u63db\u3048\u30671\u7a2e\u985e\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3060\u3051\u304c\u30e9\u30f3\u30c0\u30e0\u306b\u767a\u73fe\u3057\u307e\u3059\u3002\u3053\u306e\u767a\u8272\u306e\u69d8\u5b50\u3067\u76ee\u7684\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u5c65\u6b74\u3084\u305d\u306e\u72b6\u6cc1\u3092\u63a8\u5bdf\u3067\u304d\u307e\u3059\u3002\u305d\u3053\u3067Confetti\u30de\u30a6\u30b9\u3068Cre-RANKL\u30de\u30a6\u30b9\u3092\u304b\u3051\u3042\u308f\u305b\u3001\u30ea\u30f3\u30d1\u7bc0\u306e\u30b9\u30c8\u30ed\u30fc\u30de\u7d30\u80de\u3092\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30fc\u30bf\u30fc\u3067\u89e3\u6790\u3057\u307e\u3057\u305f\u3002\u3053\u306e\u89e3\u6790\u3067\u306f\u30014\u8272\u306e\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u4ee5\u5916\u306b\u3082\u8840\u7403\u7cfb\u3084\u30b9\u30c8\u30ed\u30fc\u30de\u7d30\u80de\u306e\u30de\u30fc\u30ab\u30fc\u7b49\u5408\u8a089\u8272\u3092\u8b58\u5225\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3082\u30b9\u30c8\u30ed\u30fc\u30de\u7d30\u80de\u306e\u5272\u5408\u306f\u975e\u5e38\u306b\u5c0f\u3055\u3044\u305f\u3081\u3001\u9ad8\u6d41\u901f\u3067\u7cbe\u5ea6\u3088\u304f\u89e3\u6790\u3067\u304d\u6700\u59274\u30ec\u30fc\u30b6\u30fc\u306714\u8272\u691c\u51fa\u53ef\u80fd\u306aAttune NxT Acoustic Focusing 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style=\"font-size: 12pt\">\u30b9\u30c8\u30ec\u30b9\u306e\u306a\u3044\u30de\u30eb\u30c1\u30ab\u30e9\u30fc\u89e3\u6790\u3092\u5b9f\u73fe<\/span><br \/>\n<span style=\"font-size: 12pt\">Attune NxT Flow Cytometer<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-analysis\/flow-cytometry\/flow-cytometers\/attune-acoustic-focusing-flow-cytometer.html?CID=Social_LAB\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-4097 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/CO018483-kikai-1-e1537945174778-2.jpg\" alt=\"Attune\" width=\"250\" height=\"182\" \/><\/a>Invitrogen\u2122 Attune\u2122 NxT Flow 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\/><\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2018\u5e743\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/next.html?CID=bid_pca_fin_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_fpl5n_Social_LAB\" target=\"_blank\" rel=\"noopener\">NEXT\u306e\u30d0\u30c3\u30af\u30ca\u30f3\u30d0\u30fc\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>A trip to the small world of fetal lymph node \u6fa4\u65b0\u4e00\u90ce \u6c0f \uff08\u5317\u6d77\u9053\u5927\u5b66\u907a\u4f1d\u5b50\u75c5\u5236\u5fa1\u7814\u7a76\u6240\u611f\u67d3\u75c5\u614b\u5206\u91ce \u51c6\u6559\u6388\uff09 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content=\"2025-02-28T01:40:16+00:00\" \/>\n<meta property=\"og:image\" content=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2019\/07\/0fcf4e3bfc69e4e121a54b1bc221c21d-2.png\" \/>\n\t<meta property=\"og:image:width\" content=\"1200\" \/>\n\t<meta property=\"og:image:height\" content=\"630\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"author\" content=\"LATB Staff\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:creator\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:site\" content=\"@ThermoFisherJP\" \/>\n<meta name=\"twitter:label1\" content=\"\u57f7\u7b46\u8005\" \/>\n\t<meta name=\"twitter:data1\" content=\"LATB Staff\" \/>\n\t<meta name=\"twitter:label2\" content=\"\u63a8\u5b9a\u8aad\u307f\u53d6\u308a\u6642\u9593\" \/>\n\t<meta name=\"twitter:data2\" content=\"8\u5206\" \/>\n<script type=\"application\/ld+json\" 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