{"id":12005,"date":"2022-06-28T16:56:13","date_gmt":"2022-06-28T07:56:13","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=12005"},"modified":"2024-09-18T16:23:49","modified_gmt":"2024-09-18T07:23:49","slug":"ce_primersdesign_gsd_ts","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ce_primersdesign_gsd_ts\/","title":{"rendered":"\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u305f\u3081\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3"},"content":{"rendered":"<p>\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u6cd5\u3067\u306f\u3001PCR\u5897\u5e45\u306e\u8a2d\u8a08\u3092\u542b\u3081\u3066\u3001\u3088\u308a\u826f\u3044\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\u304c\u76ee\u7684\u9818\u57df\u306e\u5897\u5e45\u3084\u305d\u306e\u5f8c\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u6210\u529f\u306b\u6b20\u304b\u305b\u307e\u305b\u3093\u3002\u305d\u3053\u3067\u4eca\u56de\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3059\u308b\u969b\u306e\u30dd\u30a4\u30f3\u30c8\u3084\u4fbf\u5229\u306a\u30c4\u30fc\u30eb\u306b\u3064\u3044\u3066\u3001\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<br \/>\n\u30fb\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306b\u304a\u56f0\u308a\u306e\u65b9<br \/>\n\u30fb\u3053\u308c\u304b\u3089\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u308b\u65b9<br \/>\n\u30fb\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u3067\u30b7\u30b0\u30ca\u30eb\u5f37\u5ea6\u306b\u304a\u56f0\u308a\u306e\u65b9<\/p>\n<h2>\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u306b\u304a\u3051\u308b\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u4f4d\u7f6e\u3065\u3051<\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/sanger-sequencing-workflow.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7520_gsd00000_0so_blg_op_awa_og_s00_ce_primersdesign_gsd_ts_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc<\/a>\u306f\u5927\u304d\u304f\u4e8c\u3064\u306b\u5206\u985e\u3067\u304d\u307e\u3059\u30021\u3064\u306f\u30b2\u30ce\u30e0DNA\u3092\u92f3\u578b\u3068\u3059\u308b\u5834\u5408\u3067\u3001PCR\u6cd5\u3092\u7528\u3044\u3066\u76ee\u7684\u9818\u57df\u3092\u5897\u5e45\u3057\u3001\u6b21\u306b\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u5b9f\u884c\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3082\u30461\u3064\u306e\u30d7\u30e9\u30b9\u30df\u30c9DNA\u3092\u92f3\u578b\u306b\u7528\u3044\u308b\u5834\u5408\u306f\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u5b9f\u884c\u3059\u308b\u3060\u3051\u3067\u6e08\u307f\u307e\u3059\u3002<\/p>\n<p>\u30b2\u30ce\u30e0DNA\u3092\u92f3\u578b\u3068\u3057\u3066\u30b7\u30fc\u30b1\u30f3\u30b9\u3059\u308b\u5834\u5408\u306b\u3064\u3044\u3066\u6574\u7406\u3059\u308b\u3068\u3001PCR\u53cd\u5fdc\u306b\u306f\u5897\u5e45\u3059\u308b\u914d\u5217\u306e\u9818\u57df\u3092\u6c7a\u5b9a\u3059\u308b\u9806\u65b9\u5411\u3068\u9006\u65b9\u5411\u306e2\u3064\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u5fc5\u8981\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u306f1\u672c\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u307f\u304c\u53cd\u5fdc\u306b\u4f7f\u7528\u3055\u308c\u308b\u3068\u3044\u3046\u70b9\u304cPCR\u53cd\u5fdc\u3068\u306f\u7570\u306a\u308a\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u4e00\u822c\u7684\u306b\u306f\u7279\u5b9a\u306ePCR\u7523\u7269\u306b\u5bfe\u3057\u3066\u30012\u3064\u306e\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u304c\u8a2d\u5b9a\u3055\u308c\u307e\u3059\u30022\u3064\u306e\u53cd\u5fdc\u3068\u306f\u30011\u3064\u306f\u9806\u65b9\u5411\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u3001\u3082\u30461\u3064\u306f\u9006\u65b9\u5411\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u3092\u793a\u3057\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u6cd5\u306f\u3001\u4e21\u65b9\u5411\u304b\u3089\u89e3\u6790\u3092\u3059\u308b\u3053\u3068\u3067\u7cbe\u5ea6\u306e\u9ad8\u3044\u914d\u5217\u6c7a\u5b9a\u304c\u671f\u5f85\u3067\u304d\u307e\u3059\u3002<\/p>\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306f\u3001\u57fa\u672c\u7684\u306aPCR\u3001\u30b7\u30fc\u30b1\u30f3\u30b9\u3060\u3051\u3067\u306a\u304f\u3001\u30d5\u30e9\u30b0\u30e1\u30f3\u30c8\u89e3\u6790\u3084\u3001\u5b9a\u91cf\u5206\u6790\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u5b9f\u9a13\u7cfb\u306b\u95a2\u308f\u308b\u91cd\u8981\u306a\u30dd\u30a4\u30f3\u30c8\u3067\u3059\u3002<br \/>\nApplied Biosystems\u2122 BigDye\u2122 Terminator\u30b7\u30ea\u30fc\u30ba\u306eCycle Sequencing Kit\u3067\u306f\u3001PCR\u53cd\u5fdc\u306b\u7528\u3044\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306b\u3082\u6d41\u7528\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u305f\u3081\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306e\u91cd\u8981\u6027\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n<h2>\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306e\u30dd\u30a4\u30f3\u30c8\u3068\u6709\u7528\u306a\u30c4\u30fc\u30eb\u306e\u3054\u7d39\u4ecb<\/h2>\n<p>\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u8a2d\u8a08\u3059\u308b\u969b\u306b\u8003\u616e\u3059\u308b\u30dd\u30a4\u30f3\u30c8\u306f\u3044\u304f\u3064\u304b\u3042\u308a\u307e\u3059\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u9577\u3055\u306f18\u301c22\u5869\u57fa\u7a0b\u5ea6\u3067\u30c7\u30b6\u30a4\u30f3\u3057\u307e\u3059\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306eGC\u542b\u91cf\u306f50\uff05\u304b\u308955\uff05\u306b\u306a\u308b\u3088\u3046\u306b\u3057\u307e\u3059\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306e3 \u2018\u672b\u7aef\u306e\u6700\u5f8c\u306e1\u5869\u57fa\u3082\u3057\u304f\u306f2\u5869\u57fa\u3092G\u304bC\u306b\u3057\u307e\u3059\uff08GC\u30ed\u30c3\u30af\uff09\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u878d\u89e3\u6e29\u5ea6\u306f\u300150\u2103\u304b\u308955\u2103\u4f4d\u306b\u306a\u308b\u3088\u3046\u306b\u3057\u307e\u3059\u3002<br \/>\n\u30fb\u540c\u4e00\u5869\u57fa\u304c\u9023\u7d9a\u3059\u308b\u9818\u57df\u3092\u907f\u3051\u3066\u30d7\u30e9\u30a4\u30de\u30fc\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u307e\u3059\u3002<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u914d\u5217\u5185\u306b\u30824\u3064\u4ee5\u4e0a\u306e\u9023\u7d9a\u5869\u57fa\u306f\u907f\u3051\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<br \/>\n\u3053\u306e\u3088\u3046\u306b\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30c7\u30b6\u30a4\u30f3\u306f\u8003\u616e\u3059\u3079\u304d\u30dd\u30a4\u30f3\u30c8\u304c\u3044\u304f\u3064\u304b\u3042\u308a\u3001\u7c21\u5358\u3067\u306f\u306a\u3044\u305f\u3081\u3001\u30c7\u30b6\u30a4\u30f3\u3057\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306b\u3088\u3063\u3066\u306f\u3001\u5b9f\u9a13\u304c\u3046\u307e\u304f\u3044\u304b\u305a\u3001\u4f59\u8a08\u306a\u6642\u9593\u3084\u30b3\u30b9\u30c8\u304c\u304b\u304b\u3063\u3066\u3057\u307e\u3046\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u3001\u30d2\u30c8\u30a8\u30af\u30bd\u30fc\u30e0\u3068\u30d2\u30c8\u30df\u30c8\u30b3\u30f3\u30c9\u30ea\u30a2\u30b2\u30ce\u30e0\u3092\u5b9f\u9a13\u5bfe\u8c61\u3068\u3055\u308c\u3066\u3044\u308b\u65b9\u306b\u306f\u3001\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u306e\u691c\u7d22\u30c4\u30fc\u30ebApplied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/sequencing\/sanger-sequencing\/pre-designed-primers-pcr-sanger-sequencing.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7520_gsd00000_0so_blg_op_awa_og_s00_ce_primersdesign_gsd_ts_Social_LAB\" target=\"_blank\" rel=\"noopener\">Primer Designer\u30c4\u30fc\u30eb<\/a>\u306e\u3054\u5229\u7528\u3092\u304a\u52e7\u3081\u3057\u3066\u3044\u307e\u3059\u3002\u30d2\u30c8\u4ee5\u5916\u3092\u5bfe\u8c61\u306b\u5b9f\u9a13\u5bfe\u8c61\u3068\u3055\u308c\u3066\u3044\u308b\u65b9\u306f\u30af\u30e9\u30a6\u30c9\u30b5\u30fc\u30d3\u30b9\u306e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/oligonucleotides-primers-probes-genes\/custom-dna-oligos\/oligo-design-tools\/oligoperfect.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7520_gsd00000_0so_blg_op_awa_og_s00_ce_primersdesign_gsd_ts_Social_LAB\" target=\"_blank\" rel=\"noopener\">OligoPerfect\u30c7\u30b6\u30a4\u30ca\u30fc<\/a>\u3092\u3054\u5229\u7528\u3044\u305f\u3060\u3051\u307e\u3059\u3002<\/p>\n<h2>\u30ab\u30b9\u30bf\u30e0\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u691c\u8a3c<\/h2>\n<p>\u30ab\u30b9\u30bf\u30e0\u30c7\u30b6\u30a4\u30f3\u3055\u308c\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u304c\u6709\u7528\u304b\u3069\u3046\u304b\u691c\u8a0e\u3044\u305f\u3060\u304f\u306b\u306f\u3001\u5f53\u793e\u306e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/thermo-scientific-web-tools\/multiple-primer-analyzer.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7520_gsd00000_0so_blg_op_awa_og_s00_ce_primersdesign_gsd_ts_Social_LAB\" target=\"_blank\" rel=\"noopener\">Multiple Primer Analyzer<\/a>\u3092\u3054\u5229\u7528\u3044\u305f\u3060\u3051\u307e\u3059\u3002Multiple Primer Analyzer\u306f1\u672c\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u304b\u3089\u8907\u6570\u672c\u306e\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u306eTm\u5024\u3092\u7b97\u51fa\u3059\u308b\u4ee5\u5916\u306b\u3082\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u5f62\u6210\u306e\u30b7\u30df\u30e5\u30ec\u30fc\u30b7\u30e7\u30f3\u304c\u53ef\u80fd\u3067\u3059\u3002<\/p>\n<p>Multiple Primer Analyzer\u306e\u4f7f\u3044\u65b9<br \/>\n\u2460\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217\u3092FASTA\u5f62\u5f0f\u3067\u767b\u9332\u3057\u307e\u3059\u3002<br \/>\n&gt;\u30d7\u30e9\u30a4\u30de\u30fc\u540d1<br \/>\n\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217<br \/>\n&gt;\u30d7\u30e9\u30a4\u30de\u30fc\u540d2<br \/>\n\u30d7\u30e9\u30a4\u30de\u30fc\u914d\u5217<br \/>\n\u2026<br \/>\n\u2461\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u6fc3\u5ea6\u3001\u5869\u6fc3\u5ea6\u3001\u30c0\u30a4\u30de\u30fc\u691c\u51fa\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u3092\u8a2d\u5b9a\u3057\u307e\u3059\u3002<br \/>\n\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u3068\u3057\u3066\u78ba\u8a8d\u3059\u308b\u5834\u5408\u306f\u3001\u30d7\u30e9\u30a4\u30de\u30fc\u6fc3\u5ea6\u30920.16 \u00b5M\u3001\u5869\u6fc3\u5ea6\u309250 mM\u3001\u30d1\u30e9\u30e1\u30fc\u30bf\u30fc\u30923\u3067\u8a08\u7b97\u3057\u307e\u3059\u3002<br \/>\n\u2462Tm\u5024\u306a\u3069\u306e\u7b97\u51fa\u7d50\u679c\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002<br \/>\n\u7d50\u679c\u306f\u30c6\u30ad\u30b9\u30c8\u30b3\u30d4\u30fc\uff08Ctrl+A\u3001Ctrl+C\uff09\u3057\u3001\u8868\u8a08\u7b97\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u306a\u3069\u306b\u30da\u30fc\u30b9\u30c8\uff08Ctrl+V\uff09\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br \/>\n\u2463\u30d7\u30e9\u30a4\u30de\u30fc\u30c0\u30a4\u30de\u30fc\u304c\u4e88\u6e2c\u3055\u308c\u305f\u5834\u5408\u3001\u7d50\u679c\u304c\u8868\u793a\u3055\u308c\u307e\u3059\u3002<br \/>\n\u7d50\u679c\u306f\u30c6\u30ad\u30b9\u30c8\u30b3\u30d4\u30fc\uff08Ctrl+A\u3001Ctrl+C\uff09\u3057\u3001\u30c6\u30ad\u30b9\u30c8\u30a8\u30c7\u30a3\u30bf\u30fc\u306a\u3069\u306b\u30da\u30fc\u30b9\u30c8\uff08Ctrl+V\uff09\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-large wp-image-12017\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/06\/Fig1-Multiple-Primer-Analyzer-737x1024.jpg\" alt=\"\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\" width=\"737\" height=\"1024\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/06\/Fig1-Multiple-Primer-Analyzer-737x1024.jpg 737w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/06\/Fig1-Multiple-Primer-Analyzer-216x300.jpg 216w, 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href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/oligonucleotides-primers-probes-genes\/custom-dna-oligos\/oligo-design-tools\/oligoperfect.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7520_gsd00000_0so_blg_op_awa_og_s00_ce_primersdesign_gsd_ts_Social_LAB\" target=\"_blank\" rel=\"noopener\">OligoPerfect\u30c7\u30b6\u30a4\u30ca\u30fc<\/a>\u304c\u3042\u308a\u307e\u3059<br \/>\n\u30fb\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u306e\u691c\u8a3c\u306b\u306f\u7121\u6599\u3067\u4f7f\u3048\u308b<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/brands\/thermo-scientific\/molecular-biology\/molecular-biology-learning-center\/molecular-biology-resource-library\/thermo-scientific-web-tools\/multiple-primer-analyzer.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7520_gsd00000_0so_blg_op_awa_og_s00_ce_primersdesign_gsd_ts_Social_LAB\" target=\"_blank\" rel=\"noopener\">Multiple Primer Analyzer<\/a>\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<p>\u7121\u6599\u30c4\u30fc\u30eb\u3092\u7528\u3044\u305f\u30d7\u30e9\u30a4\u30de\u30fc\u30c7\u30b6\u30a4\u30f3\u3067\u304a\u56f0\u308a\u306e\u65b9\u306f\u3001\u305c\u3072<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/products-and-services\/services\/technical-services\/application-design-service.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7520_gsd00000_0so_blg_op_awa_og_s00_ce_primersdesign_gsd_ts_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u30c7\u30b6\u30a4\u30f3\u30b5\u30fc\u30d3\u30b9<\/a>\u3092\u691c\u8a0e\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n<h2>\u3010\u7121\u6599\u516c\u958b\u4e2d\u3011\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u57fa\u790e<\/h2>\n<p>\u3053\u306e\u30b1\u30df\u30b9\u30c8\u30ea\u30fc\u30ac\u30a4\u30c9\u3067\u306f\u3001\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u304b\u3089\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u307e\u3067\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u57fa\u672c\u539f\u7406\u3092\u5b66\u3079\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/sanger-sequencing-guide-download.html?CID=gsd_cap_lcs_r04_jp_cp1425_pjt7520_gsd00000_0so_blg_op_awa_og_s00_ce_primersdesign_gsd_ts_Social_LAB\" target=\"_blank\" rel=\"noopener 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