{"id":1202,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_34_2_gibcoexpi\/"},"modified":"2023-12-27T17:56:32","modified_gmt":"2023-12-27T08:56:32","slug":"user_voice_34_2_gibcoexpi","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_34_2_gibcoexpi\/","title":{"rendered":"\u52d5\u7269\u7d30\u80de\u767a\u73fe\u7cfb\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u751f\u7523\u652f\u63f4\u304b\u3089\u5275\u85ac\u3092\u30b5\u30dd\u30fc\u30c8\uff08\u5927\u962a\u5927\u5b66\u86cb\u767d\u8cea\u89e3\u6790\u5148\u7aef\u7814\u7a76\u30bb\u30f3\u30bf\u30fc \u4e09\u539f\u6075\u7f8e\u5b50\u6c0f\u3001\u6d77\u6d25\u6b63\u8ce2\u6c0f\u3001\u6709\u68ee\u8cb4\u592b\u6c0f\uff09-\u300cNEXT\u300d2016\u5e743\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2 style=\"text-align: left\"><span style=\"font-size: 12pt\">\u4e00\u904e\u6027\u5927\u91cf\u767a\u73fe\u7cfbExpi293 Expression System\u3067\u9ad8\u96e3\u5ea6\u30bf\u30f3\u30d1\u30af\u8cea\u3082\u52b9\u7387\u3088\u304f\u7cbe\u88fd<\/span><\/h2>\n<p><strong>\u4e09\u539f\u6075\u7f8e\u5b50\u6c0f\uff08\u5927\u962a\u5927\u5b66\u86cb\u767d\u8cea\u7814\u7a76\u6240\u9644\u5c5e\u86cb\u767d\u8cea\u89e3\u6790\u5148\u7aef\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7279\u4efb\u7814\u7a76\u54e1\uff09<br \/>\n\u6d77\u6d25\u6b63\u8ce2\u6c0f\uff08\u540c\u30bb\u30f3\u30bf\u30fc\u7279\u4efb\u52a9\u6559\uff09<br \/>\n\u6709\u68ee\u8cb4\u592b\u6c0f\uff08\u540c\u30bb\u30f3\u30bf\u30fc\u7279\u4efb\u7814\u7a76\u54e1\uff09<\/strong><\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/DSC07442.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3668 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/DSC07442.jpg\" alt=\"\u4e09\u539f\u6075\u7f8e\u5b50\u6c0f\uff08\u5927\u962a\u5927\u5b66\u86cb\u767d\u8cea\u7814\u7a76\u6240\u9644\u5c5e\u86cb\u767d\u8cea\u89e3\u6790\u5148\u7aef\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7279\u4efb\u7814\u7a76\u54e1\uff09\" width=\"250\" height=\"165\" \/><\/a>\u300c\u7121\u8840\u6e05\u3067\u57f9\u990a\u53ef\u80fd\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u7cfb\u3092\u63a2\u3057\u3066\u3044\u307e\u3057\u305f\u3002\u8a66\u3057\u306bGibco\u2122 Expi293\u2122 Expression System\u3092\u4f7f\u3063\u3066\u307f\u305f\u3068\u3053\u308d\u3001\u5f93\u6765\u3088\u308a\u308215\u500d\u3082\u767a\u73fe\u91cf\u304c\u5411\u4e0a\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3082\u3042\u308a\u307e\u3057\u305f\u300d\u3068\u8a9e\u308b\u306e\u306f\u3001\u5927\u962a\u5927\u5b66\u306e\u4e09\u539f\u6075\u7f8e\u5b50\u6c0f\uff08\u5199\u771f\u524d\u5217\uff09\u3002\u4e09\u539f\u6c0f\u304c\u6240\u5c5e\u3059\u308b\u7814\u7a76\u5ba4\u306f\u300113\u5e74\u307b\u3069\u524d\u3088\u308a\u52d5\u7269\u7d30\u80de\u3067\u306e\u767a\u73fe\u7cfb\u3092\u4f7f\u3044\u3053\u306a\u3057\u3066\u304d\u305f\u5b9f\u7e3e\u304b\u3089\u3001\u65e5\u672c\u533b\u7642\u7814\u7a76\u958b\u767a\u6a5f\u69cb\u306b\u3088\u308b\u300c\u5275\u85ac\u652f\u63f4\u6280\u8853\u57fa\u76e4\u30d7\u30e9\u30c3\u30c8\u30d5\u30a9\u30fc\u30e0\u4e8b\u696d\u300d\u306e\u4e00\u74b0\u3068\u3057\u3066\u3001\u5275\u85ac\u306b\u5f79\u7acb\u3064\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u751f\u7523\u306e\u652f\u63f4\u3084\u6280\u8853\u958b\u767a\u3092\u62c5\u5f53\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u96e3\u767a\u73fe\u6027\u30bf\u30f3\u30d1\u30af\u8cea\u3082\u54fa\u4e73\u985e\u7d30\u80de\u767a\u73fe\u7cfb\u3067\u53ef\u80fd\u306b<\/span><\/h2>\n<p>\u5168\u56fd\u306e\u7814\u7a76\u6a5f\u95a2\u3078\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u652f\u63f4\u3092\u884c\u3046\u4e2d\u3067\u3001\u3088\u308a\u9ad8\u53ce\u91cf\u3067\u7cbe\u88fd\u3082\u697d\u306a\u767a\u73fe\u7cfb\u3092\u691c\u8a0e\u3057\u59cb\u3081\u305f\u4e09\u539f\u6c0f\u3002\u300c2\u5e74\u524d\u304b\u3089Expi293 Expression System\u3092\u4f7f\u3044\u59cb\u3081\u307e\u3057\u305f\u3002\u63a5\u7740\u7d30\u80de\u3067\u306f\u9762\u7a4d\u306e\u5236\u7d04\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u6d6e\u904a\u7d30\u80de\u3067\u9ad8\u5bc6\u5ea6\u306b\u57f9\u990a\u3067\u304d\u308b\u305f\u3081\u3001\u52d5\u7269\u7d30\u80de\u306b\u304a\u3051\u308b\u4e00\u904e\u6027\u5927\u91cf\u767a\u73fe\u7cfb\u3068\u3057\u3066\u975e\u5e38\u306b\u6709\u7528\u3067\u3059\u3002\u307e\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3082\u3088\u308a\u307e\u3059\u304c\u3001\u5927\u8178\u83cc\u3084\u6606\u866b\u7d30\u80de\u3067\u306f\u767a\u73fe\u3057\u306a\u304b\u3063\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u306b\u3082\u6210\u529f\u3057\u307e\u3057\u305f\u300d\u3068\u8a9e\u308a\u307e\u3059\u3002\u540c\u3058\u7814\u7a76\u5ba4\u306e\u6d77\u6d25\u6b63\u8ce2\u6c0f\uff08\u5199\u771f\u53f3\uff09\u306f\u3001\u300c\u52d5\u7269\u7d30\u80de\u3092\u4f7f\u3046\u30e1\u30ea\u30c3\u30c8\u306f\u7ffb\u8a33\u5f8c\u4fee\u98fe\u306b\u3082\u3042\u308a\u307e\u3059\u3002\u5927\u8178\u83cc\u3067\u306f\u7ffb\u8a33\u5f8c\u4fee\u98fe\u306f\u306a\u304f\u3001\u6606\u866b\u7d30\u80de\u3067\u3082\u7cd6\u9396\u306e\u4ed8\u304d\u65b9\u304c\u30d2\u30c8\u3068\u306f\u7570\u306a\u308b\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u70b9\u3001\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u306fHEK293\u7d30\u80de\u304c\u30d9\u30fc\u30b9\u306a\u306e\u3067\u3001\u30d2\u30c8\u7531\u6765\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6b63\u78ba\u306b\u3001\u5b89\u5b9a\u3057\u3066\u767a\u73fe\u3059\u308b\u7cfb\u3068\u3057\u3066\u671f\u5f85\u3067\u304d\u307e\u3059\u3002\u5b9f\u969b\u306b\u3001\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u3066\u751f\u7523\u3057\u305f\u3042\u308b\u7d30\u80de\u5897\u6b96\u56e0\u5b50\u304c\u6d3b\u6027\u3092\u6301\u3064\u3053\u3068\u304c\u78ba\u8a8d\u3067\u304d\u3066\u304a\u308a\u3001\u69cb\u9020\u89e3\u6790\u306b\u3082\u554f\u984c\u306a\u304f\u4f7f\u3048\u3066\u3044\u307e\u3059\u300d\u3068\u7d9a\u3051\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u4e00\u904e\u6027\u767a\u73fe\u3067\u6297\u4f53\u3092\u5927\u91cf\u304b\u3064\u77ed\u671f\u9593\u306b\u7cbe\u88fd<\/span><\/h2>\n<p>\u4e3b\u306b\u9ad8\u4ed8\u52a0\u4fa1\u5024\u6297\u4f53\u306e\u4f5c\u88fd\u3084\u30a8\u30f3\u30b8\u30cb\u30a2\u30ea\u30f3\u30b0\u3092\u62c5\u5f53\u3059\u308b\u6709\u68ee\u8cb4\u592b\u6c0f\uff08\u5199\u771f\u5de6\uff09\u306f\u3001\u300c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u6676\u69cb\u9020\u89e3\u6790\u306b\u306f\u3001\u4e00\u822c\u7684\u306b\u3001\u6700\u521d\u306b1 mg\u4ee5\u4e0a\u306e\u8a66\u6599\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u3053\u308c\u307e\u3067\u306f\u5b89\u5b9a\u767a\u73fe\u682a\u3092\u69cb\u7bc9\u3057\u3066\u5927\u91cf\u57f9\u990a\u3057\u3066\u7cbe\u88fd\u3059\u308b\u3053\u3068\u304c\u4e00\u822c\u7684\u3067\u3057\u305f\u304c\u3001\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u3046\u30681 L\u306e\u57f9\u5730\u63db\u7b97\u3067120 mg\u306e\u6297\u4f53\u3092\u56de\u53ce\u3067\u304d\u305f\u4f8b\u3082\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3082\u6297\u4f53\u7cbe\u88fd\u306b\u306f\u3001Protein A\u3084G\u306e\u30ab\u30e9\u30e0\u3092\u4f7f\u3046\u306e\u304c\u4e3b\u6d41\u3067\u3042\u308a\u3001\u8840\u6e05\u304b\u3089\u306e\u6297\u4f53\u6df7\u5165\u306e\u30ea\u30b9\u30af\u304c\u3042\u308a\u307e\u3057\u305f\u304c\u3001\u7121\u8840\u6e05\u57f9\u5730\u306a\u306e\u3067\u7cbe\u88fd\u304c\u697d\u306b\u306a\u308a\u307e\u3057\u305f\u300d\u3068\u8a9e\u308a\u307e\u3059\u3002\u6d77\u6d25\u6c0f\u3082\u300c\u5b89\u5b9a\u767a\u73fe\u7d30\u80de\u682a\u306e\u6a39\u7acb\u306b\u306f\u901a\u5e381,2\u304b\u6708\u304b\u304b\u308a\u307e\u3059\u304c\u3001\u4e00\u904e\u6027\u767a\u73fe\u3067\u3042\u308c\u3070\u3001\u6700\u77ed\u30671\u9031\u9593\u304b\u308910\u65e5\u3067\u7d50\u6676\u5316\u307e\u3067\u9032\u3081\u308b\u3053\u3068\u3082\u53ef\u80fd\u3002\u6606\u866b\u7d30\u80de\u3067\u306e\u767a\u73fe\u3082\u3001\u30a6\u30a4\u30eb\u30b9\u8abf\u88fd\u306b\u624b\u9593\u3084\u6642\u9593\u304c\u304b\u304b\u308a\u307e\u3059\u300d\u3068\u4e00\u904e\u6027\u767a\u73fe\u306e\u30e1\u30ea\u30c3\u30c8\u3092\u6307\u6458\u3057\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u69cb\u9020\u751f\u7269\u5b66\u304b\u3089\u306e\u65b0\u305f\u306a\u30a2\u30d7\u30ed\u30fc\u30c1<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/1-185.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3470\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/1-185.jpg\" alt=\"\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u91cf\u306e\u6bd4\u8f03\" width=\"328\" height=\"266\" \/><\/a>\u53d7\u8a17\u652f\u63f4\u3068\u4e26\u884c\u3057\u3066\u3001\u7814\u7a76\u5ba4\u3067\u306f\u795e\u7d4c\u7cfb\u3084\u514d\u75ab\u7cfb\u306a\u3069\u306e\u69d8\u3005\u306a\u7d30\u80de\u306b\u304a\u3051\u308b\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u5206\u5b50\u3092\u69cb\u9020\u751f\u7269\u5b66\u624b\u6cd5\u304b\u3089\u7814\u7a76\u4e2d\u3002\u6d77\u6d25\u6c0f\u306f\u300c\u3053\u308c\u307e\u3067\u306f\u91cf\u7684\u306a\u5236\u9650\u3082\u3042\u308a\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u90e8\u5206\u7684\u306b\u3057\u304b\u69cb\u9020\u89e3\u6790\u3067\u304d\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u3048\u3070\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u4e38\u3054\u3068\u89e3\u6790\u3059\u308b\u30c1\u30e3\u30f3\u30b9\u3082\u5897\u3048\u305d\u3046\u3067\u3059\u3002\u307e\u305f\u3001\u7cd6\u9396\u3092\u5747\u4e00\u5316\u3059\u308b\u76ee\u7684\u3067\u30ad\u30d5\u30cd\u30b7\u30f3\u3068\u3044\u3046\u85ac\u5264\u3092\u57f9\u5730\u306b\u6dfb\u52a0\u3059\u308b\u65b9\u6cd5\u304cExpi293 Expression System\u306b\u304a\u3044\u3066\u6709\u52b9\u3067\u3042\u308b\u3053\u3068\u3082\u308f\u304b\u308a\u3001\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u6676\u5316\u306b\u5411\u3051\u305f\u30a2\u30d7\u30ed\u30fc\u30c1\u6cd5\u3082\u5897\u3048\u305d\u3046\u3067\u3059\u3002\u5f53\u7814\u7a76\u5ba4\u3067\u306f\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u3092\u7528\u3044\u3066\u3001\u3059\u3067\u306b\u53d7\u7406\u3055\u308c\u305f\u8ad6\u6587\u203b\u3084\u6e96\u5099\u4e2d\u306e\u8ad6\u6587\u3082\u3042\u308a\u3001\u7814\u7a76\u306e\u30d9\u30fc\u30b9\u3068\u3057\u3066\u4eca\u5f8c\u3082\u6d3b\u7528\u3057\u305f\u3044\u300d\u3068\u8a71\u3057\u307e\u3059\u3002<br \/>\n<span style=\"font-size: 8pt\">\u203b&#8221;Active and water-soluble form of lipidated Wnt protein is maintained by a serum glycoprotein afamin\/\u03b1-albumin&#8221; eLife, in press \uff082016\uff09<\/span><\/p>\n<h2><span style=\"font-size: 12pt\">\u5927\u91cf\u767a\u73fe\u3092\u53ef\u80fd\u306b\u3057\u305f\u54fa\u4e73\u985e\u7d30\u80de\u306e\u4e00\u904e\u6027\u767a\u73fe\u30b7\u30b9\u30c6\u30e0<\/span><br \/>\n<span style=\"font-size: 12pt\">Gibco Expi\u30b7\u30ea\u30fc\u30ba \uff083\u88fd\u54c1\uff09<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/p6Expi293A14635-Kit-10in-2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-3669 size-full alignnone\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/p6Expi293A14635-Kit-10in-2.jpg\" alt=\"Gibco Expi\u30b7\u30ea\u30fc\u30ba \" width=\"250\" height=\"185\" \/><\/a><\/p>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-biology\/protein-expression\/mammalian-protein-expression\/transient-mammalian-protein-expression\/expi293-expression-system.html?cid=bid_clb_pex_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pe1n_Social_LAB\" target=\"_blank\" rel=\"noopener\">Expi293 Expression System<\/a>\uff1a HEK293\u7d30\u80de\u30d9\u30fc\u30b9\u3067\u6700\u59271g\/L\u306e\u767a\u73fe<\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-biology\/protein-expression\/mammalian-protein-expression\/transient-mammalian-protein-expression\/expicho-expression-system.html?cid=bid_clb_pex_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pe1n_Social_LAB\" target=\"_blank\" rel=\"noopener\">ExpiCHO Expression System<\/a>\uff1a CHO\u7d30\u80de\u30d9\u30fc\u30b9\u3067\u6700\u59273g\/L\u306e\u767a\u73fe<\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-biology\/protein-expression\/mammalian-protein-expression\/membranepro-functional-protein-expression-systems.html?cid=bid_clb_pex_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pe1n_Social_LAB\" target=\"_blank\" rel=\"noopener\">Expi293 MembranePro Expression System<\/a>\uff1a \u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6a5f\u80fd\u89e3\u6790\u306b\u6700\u9069<\/li>\n<\/ul>\n<h2><span style=\"font-size: 12pt\">\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=bid_clb_pex_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pe1n_Social_LAB\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-3628\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/foregroundimg.img_.full_.high_-2.gif\" alt=\"NEXT2016\u5e743\u6708\u53f7\" width=\"167\" height=\"235\" \/><\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2016\u5e743\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=bid_clb_pex_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_pe1n_Social_LAB\" target=\"_blank\" rel=\"noopener\">NEXT\u306e\u30d0\u30c3\u30af\u30ca\u30f3\u30d0\u30fc\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u4e00\u904e\u6027\u5927\u91cf\u767a\u73fe\u7cfbExpi293 Expression System\u3067\u9ad8\u96e3\u5ea6\u30bf\u30f3\u30d1\u30af\u8cea\u3082\u52b9\u7387\u3088\u304f\u7cbe\u88fd \u4e09\u539f\u6075\u7f8e\u5b50\u6c0f\uff08\u5927\u962a\u5927\u5b66\u86cb\u767d\u8cea\u7814\u7a76\u6240\u9644\u5c5e\u86cb\u767d\u8cea\u89e3\u6790\u5148\u7aef\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7279\u4efb\u7814\u7a76\u54e1\uff09 \u6d77\u6d25\u6b63\u8ce2\u6c0f\uff08\u540c\u30bb\u30f3\u30bf\u30fc\u7279\u4efb\u52a9\u6559\uff09 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