{"id":1223,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_35_1_superscript\/"},"modified":"2023-12-28T09:31:38","modified_gmt":"2023-12-28T00:31:38","slug":"user_voice_35_1_superscript","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_35_1_superscript\/","title":{"rendered":"\u304c\u3093\u6291\u5236\u907a\u4f1d\u5b50p53\u306e\u65b0\u898f\u6a19\u7684\u907a\u4f1d\u5b50IER5\u306f\u304c\u3093\u7d30\u80de\u306e\u30b9\u30c8\u30ec\u30b9\u56de\u907f\u306b\u306f\u305f\u3089\u304f\uff08\u56fd\u7acb\u304c\u3093\u7814\u7a76\u30bb\u30f3\u30bf\u30fc \u5927\u6728\u7406\u6075\u5b50\u6c0f\u3001\u65e9\u7a32\u7530\u5927\u5b66\u5148\u9032\u7406\u5de5\u5b66\u7814\u7a76\u79d1 \u6d45\u91ce\u826f\u5247\u6c0f\uff09-\u300cNEXT\u300d2016\u5e745\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2 style=\"text-align: left\"><span style=\"font-size: 12pt\">\u767a\u73fe\u89e3\u6790\u306fSuperScript Reverse Transcriptase\uff08RT\uff09\u3067\u52b9\u7387\u3088\u304f<\/span><\/h2>\n<p><strong>\u5927\u6728\u7406\u6075\u5b50\u6c0f\uff08 \u56fd\u7acb\u7814\u7a76\u958b\u767a\u6cd5\u4eba\u56fd\u7acb\u304c\u3093\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7814\u7a76\u54e1\uff09<\/strong><br \/>\n<strong>\u6d45\u91ce\u826f\u5247\u6c0f\uff08 \u65e9\u7a32\u7530\u5927\u5b66\u5148\u9032\u7406\u5de5\u5b66\u7814\u7a76\u79d1\u535a\u58eb\u8ab2\u7a0b\uff09<\/strong><\/p>\n<p><span style=\"font-size: 14pt\"><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/P5_image-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3681 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/P5_image-1.jpg\" alt=\"\u5927\u6728\u7406\u6075\u5b50\u6c0f\uff08 \u56fd\u7acb\u7814\u7a76\u958b\u767a\u6cd5\u4eba\u56fd\u7acb\u304c\u3093\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u7814\u7a76\u54e1\uff09\" width=\"250\" height=\"162\" \/><\/a><\/span>\u7d30\u80de\u5468\u671f\u306e\u505c\u6b62\u3084\u30a2\u30dd\u30c8\u30fc\u30b7\u30b9\u306a\u3069\u3092\u5f15\u304d\u8d77\u3053\u3059\u8ee2\u5199\u56e0\u5b50\u3068\u3057\u3066\u77e5\u3089\u308c\u308b\u3001\u304c\u3093\u6291\u5236\u907a\u4f1d\u5b50p53\u3002\u305d\u306e\u6a19\u7684\u3068\u306a\u308b\u907a\u4f1d\u5b50\u306f\u591a\u5c90\u306b\u308f\u305f\u308a\u307e\u3059\u3002\u300c\u79c1\u305f\u3061\u306f10\u5e74\u307b\u3069\u524d\u306b235\u500b\u306ep53\u6a19\u7684\u907a\u4f1d\u5b50\u3092\u540c\u5b9a\u3057\u307e\u3057\u305f\u300d\u3068\u8a9e\u308b\u306e\u306f\u3001\u56fd\u7acb\u304c\u3093\u7814\u7a76\u30bb\u30f3\u30bf\u30fc\u4e3b\u4efb\u7814\u7a76\u54e1\u306e\u5927\u6728\u7406\u6075\u5b50\u6c0f\u3002\u5927\u6728\u6c0f\u306f\u3001p53\u306e\u7814\u7a76\u309220\u5e74\u306b\u4e98\u3063\u3066\u7d9a\u3051\u3001\u4eca\u56de\u65b0\u305f\u306b\u304c\u3093\u5897\u6b96\u306b\u5fc5\u8981\u306a\u907a\u4f1d\u5b50\u3092\u767a\u898b\u3057\u307e\u3057\u305f\u3002\u300cp53\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u306b\u5f37\u304f\u4f9d\u5b58\u3059\u308b\u3001\u6a5f\u80fd\u4e0d\u660e\u306ap53\u6a19\u7684\u907a\u4f1d\u5b50IER5\u306b\u7740\u76ee\u3057\u307e\u3057\u305f\u3002\u305d\u3057\u3066\u3001IER5\u306f\u71b1\u30b7\u30e7\u30c3\u30af\u30bf\u30f3\u30d1\u30af\u8cea\uff08HSP\uff09\u306e\u8ee2\u5199\u56e0\u5b50\u3067\u3042\u308bHSF1\u3068\u7d50\u5408\u3001\u6d3b\u6027\u5316\u3055\u305b\u308b\u3053\u3068\u3067\u3001\u4f4e\u9178\u7d20\u3084\u6804\u990a\u4e0d\u8db3\u3068\u3044\u3063\u305f\u30b9\u30c8\u30ec\u30b9\u304b\u3089\u304c\u3093\u7d30\u80de\u3092\u4fdd\u8b77\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u300d\u3068\u8a9e\u308a\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u672a\u77e5\u306a\u308b\u907a\u4f1d\u5b50IER5\u3068\u304c\u3093\u7d30\u80de\u5897\u6b96\u3068\u306e\u95a2\u308f\u308a\u304c\u660e\u3089\u304b\u306b<\/span><\/h2>\n<p>\u4eca\u56de\u306e\u7814\u7a76\u3067\u3001IER5\u306f\u814e\u304c\u3093\u3001\u5927\u8178\u304c\u3093\u3001\u81b5\u304c\u3093\u306a\u3069\u69d8\u3005\u306a\u304c\u3093\u3067\u767a\u73fe\u304c\u4e0a\u6607\u3057\u3066\u3044\u308b\u307b\u304b\u3001\u767a\u73fe\u3092\u6291\u5236\u3059\u308b\u3068\u304c\u3093\u7d30\u80de\u306e\u5897\u6b96\u304c\u6291\u5236\u3055\u308c\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3057\u305f\u3002\u300c\u3055\u3089\u306b\u8208\u5473\u6df1\u3044\u3053\u3068\u306b\u3001IER5\u306b\u3088\u308bHSF1\u6d3b\u6027\u5316\u30e1\u30ab\u30cb\u30ba\u30e0\u3092\u8a73\u7d30\u306b\u89e3\u6790\u3059\u308b\u3068\u3001\u8131\u30ea\u30f3\u9178\u5316\u3067\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u304c\u308f\u304b\u3063\u305f\u3093\u3067\u3059\u3002\u8cea\u91cf\u5206\u6790\uff08LC-MS\uff09\u304b\u3089\u3001\u30ea\u30f3\u9178\u5316\u90e8\u4f4d\u306e\u540c\u5b9a\u3082\u3067\u304d\u307e\u3057\u305f\u3002\u3053\u308c\u307e\u3067HSF1\u306f\u3001\u30ea\u30f3\u9178\u5316\u30ec\u30d9\u30eb\u306e\u9ad8\u3044\u72b6\u614b\u3067\u6d3b\u6027\u5316\u3059\u308b\u3053\u3068\u304c\u77e5\u3089\u308c\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u4eca\u56de\u306e\u3088\u3046\u306b\u8131\u30ea\u30f3\u9178\u5316\u3067\u30ea\u30f3\u9178\u5316\u30ec\u30d9\u30eb\u304c\u4f4e\u4e0b\u3057\u305f\u72b6\u614b\u3067\u3082\u6d3b\u6027\u5316\u3059\u308b\u3068\u3044\u3046\u5831\u544a\u306f\u521d\u3081\u3066\u3067\u3057\u305f\u300d\u3002\u3053\u308c\u307e\u3067\u8907\u6570\u306ep53\u6a19\u7684\u907a\u4f1d\u5b50\u306e\u6a5f\u80fd\u3092\u660e\u3089\u304b\u306b\u3057\u3066\u304d\u305f\u5927\u6728\u6c0f\u3002\u300c\u6a5f\u80fd\u672a\u77e5\u306e\u907a\u4f1d\u5b50\u306e\u7814\u7a76\u306f\u3001\u7d50\u679c\u304c\u4f34\u3046\u3068\u306f\u9650\u3089\u305a\u3001\u96e3\u3057\u3044\u9762\u3082\u3042\u308a\u307e\u3059\u304c\u3001\u7814\u7a76\u8005\u3068\u3057\u3066\u6311\u6226\u3059\u3079\u304d\u3053\u3068\u300d\u3068\u8a9e\u308a\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u767a\u73fe\u89e3\u6790\u306ecDNA\u5408\u6210\u306fSuperScript RT\u3067<\/span><\/h2>\n<p>\u5404\u81d3\u5668\u3067\u306eIER5\u907a\u4f1d\u5b50\u306e\u767a\u73fe\u91cf\u306e\u8a08\u6e2c\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0\u3067\u884c\u3044\u3001cDNA\u5408\u6210\u306b\u306f\u300115\u5e74\u307b\u3069\u524d\u304b\u3089Invitrogen\u2122 SuperScript\u2122 RT\u3092\u4f7f\u7528\u3057\u3066\u304d\u305f\u3068\u8a9e\u308b\u5927\u6728\u6c0f\u3002\u300c\u3053\u3053\u305e\u3068\u3044\u3046\u6642\u306b\u306f\u4fe1\u983c\u3067\u304d\u308b\u9175\u7d20\u3067\u3059\u306d\u3002\u65b0\u767a\u58f2\u306eSuperScript \u2163 RT\u306f\u3001\u53ce\u91cf\u304c\u4f4e\u3044RNA\u3084\u3001\u5206\u89e3\u3057\u305fRNA\u3067\u3082\u9006\u8ee2\u5199\u3067\u304d\u308b\u70b9\u3092\u671f\u5f85\u3057\u3066\u3044\u307e\u3059\u3002RNA\u304c\u5206\u89e3\u3057\u3084\u3059\u3044\u81e8\u5e8a\u30b5\u30f3\u30d7\u30eb\u3092\u767a\u73fe\u89e3\u6790\u3059\u308b\u6a5f\u4f1a\u304c\u591a\u3044\u3081\u3001cDNA\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u306e\u54c1\u8cea\u306f\u3001\u305d\u306e\u5f8c\u306e\u5b9f\u9a13\u3092\u5de6\u53f3\u3057\u304b\u306d\u307e\u305b\u3093\u3002\u5b9f\u969b\u306b\u304b\u306a\u308a\u5206\u89e3\u304c\u9032\u3093\u3060\u30de\u30a6\u30b9\u81b5\u81d3\u306eRNA\u30b5\u30f3\u30d7\u30eb\u3067\u691c\u8a3c\u3057\u305f\u3068\u3053\u308d\u3001\u4ed6\u793e\u9175\u7d20\u3088\u308a\u3082\u9006\u8ee2\u5199\u53cd\u5fdc\u304c\u9032\u3080\u3053\u3068\u3092\u78ba\u8a8d\u3057\u307e\u3057\u305f\uff08\u8868\uff09\u300d\u3068\u7d9a\u3051\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/1-2-2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3503 size-medium\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/1-2-2-300x131.jpg\" alt=\"\u9006\u8ee2\u5199\u9175\u7d20\u306e\u6bd4\u8f03\" width=\"300\" height=\"131\" \/><\/a><\/p>\n<p><span style=\"font-size: 9pt\"><strong>[\u9006\u8ee2\u5199\u9175\u7d20\u306e\u6bd4\u8f03]<\/strong><\/span><br \/>\n<span style=\"font-size: 9pt\">\u5206\u89e3\u304c\u9032\u3093\u3060\u30de\u30a6\u30b9\u81b5\u81d3\u306eRNA\u3092\u9006\u8ee2\u5199\u5f8c\u3001\u5404\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3051\u308bGAPDH\u907a\u4f1d\u5b50\u767a\u73fe\u3092\u30ea\u30a2\u30eb\u30e9\u30bf\u30a4\u30e0PCR\u3067\u6e2c\u5b9a\u3001CT\u5024\u3092\u6bd4\u8f03\u3057\u307e\u3057\u305f\u3002<\/span><\/p>\n<h2><\/h2>\n<p>\u65b0\u305f\u306a\u5206\u5b50\u6a19\u7684\u85ac\u306e\u958b\u767a\u306b\u3064\u306a\u304c\u308b\u6210\u679c<\/p>\n<p>\u300c\u4eca\u5f8c\u3001IER5\u306b\u95a2\u3059\u308b\u52d5\u7269\u30e2\u30c7\u30eb\u306e\u4f5c\u51fa\u3084HSF1\u306e\u6d3b\u6027\u5316\u306b\u95a2\u4e0e\u3059\u308b\u4f4e\u30ea\u30f3\u9178\u5316\u306e\u4f5c\u7528\u6a5f\u5e8f\u3092\u8a73\u7d30\u306b\u8abf\u3079\u3001IER5\u3084HSF1\u304c\u95a2\u308f\u308bHSP\u767a\u73fe\u7d4c\u8def\u3068\u304c\u3093\u5316\u3068\u306e\u95a2\u308f\u308a\u3092\u89e3\u660e\u3057\u3066\u3044\u304d\u305f\u3044\u300d\u3068\u5927\u6728\u6c0f\u3002\u4eca\u56de\u306e\u7814\u7a76\u3092\u5171\u306b\u62c5\u5f53\u3057\u305f\u6d45\u91ce\u826f\u5247\u6c0f\u3082\u300cIER5\u306e\u6a5f\u80fd\u3092\u963b\u5bb3\u3059\u308b\u5316\u5408\u7269\u3092\u958b\u767a\u3059\u308c\u3070\u3001\u304c\u3093\u6cbb\u7642\u306e\u5206\u5b50\u6a19\u7684\u85ac\u306e\u5275\u51fa\u306b\u3064\u306a\u304c\u308a\u307e\u3059\u3002\u81e8\u5e8a\u7814\u7a76\u306b\u3064\u306a\u3052\u3066\u3044\u304d\u305f\u3044\u300d\u3068\u8a9e\u308a\u307e\u3059\u3002p53\u907a\u4f1d\u5b50\u304c\u95a2\u308f\u308b\u304c\u3093\u5316\u306e\u5206\u5b50\u30e1\u30ab\u30cb\u30ba\u30e0\u89e3\u660e\u306b\u5411\u3051\u305f\u3055\u3089\u306a\u308b\u6311\u6226\u304c\u7d9a\u304d\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">10\u5206\u9593\u3067\u5168\u3066\u3092\u9006\u8ee2\u5199\u3059\u308b<\/span><br \/>\n<span style=\"font-size: 12pt\">SuperScript \u2163 Reverse Transcriptase<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/18080044-650x600-650-2-1.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"size-full wp-image-4702 alignnone\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/18080044-650x600-650-2-1.jpg\" alt=\"SuperScript \u2163 Reverse Transcriptase\" width=\"250\" height=\"175\" \/><\/a><\/p>\n<p>\u9ad8\u6027\u80fd SuperScript \u2162 Reverse Transcriptase\u306e\u54c1\u8cea\u3092\u5168\u3066\u5f15\u304d\u7d99\u304e\u3001\u3055\u3089\u306b\u78ba\u5b9f\u6027\u3092\u9ad8\u3081\u305f\u9006\u8ee2\u5199\u9175\u7d20\u3067\u3059\u3002<\/p>\n<ul>\n<li>\u308f\u305a\u304b10\u5206\u9593\u306e\u53cd\u5fdc\u6642\u9593<\/li>\n<li>\u5206\u89e3\u3057\u305fRNA\u3067\u3082OK<\/li>\n<li>\u53cd\u5fdc\u963b\u5bb3\u5264\u306b\u5bfe\u3059\u308b\u9ad8\u3044\u8010\u6027<\/li>\n<li>\u512a\u308c\u305f\u71b1\u5b89\u5b9a\u6027\uff08\u6700\u9ad855\u2103\uff09<\/li>\n<\/ul>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/reverse-transcription\/reverse-transcriptase-enzymes\/superscript-iv-reverse-transcriptase.html?cid=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb1n_Social_LAB\" target=\"_blank\" rel=\"noopener\">SuperScript \u2163 Reverse Transcriptase\u306e\u8a73\u7d30\u60c5\u5831\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n<h2><span style=\"font-size: 12pt\">\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_mb1n_Social_LAB\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-3633\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/foregroundimg.img_.full_.high_-1-1.gif\" alt=\"NEXT2016\u5e745\u6708\u53f7\" width=\"167\" height=\"235\" \/><\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2016\u5e745\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002<br \/>\n<a 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