{"id":12461,"date":"2022-08-24T17:24:42","date_gmt":"2022-08-24T08:24:42","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=12461"},"modified":"2023-12-27T15:56:09","modified_gmt":"2023-12-27T06:56:09","slug":"basic_ngs_workshop_gsd_ts","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic_ngs_workshop_gsd_ts\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u6295\u5165\u91cf\u3092\u904e\u5270\u306b\u3057\u3066\u307f\u305f"},"content":{"rendered":"

\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306f\u907a\u4f1d\u5b50\u3092\u7db2\u7f85\u7684\u304b\u3064\u591a\u69d8\u306a\u7528\u9014\uff08\u5909\u7570\u89e3\u6790\u3084\u767a\u73fe\u89e3\u6790\u306a\u3069\uff09\u3067\u89e3\u6790\u3067\u304d\u308b\u975e\u5e38\u306b\u5f37\u529b\u306a\u5206\u6790\u88c5\u7f6e\u3067\u3059\u3002\u4e00\u6614\u524d\u307e\u3067NGS\uff08Next Generation Sequencing\uff09\u306f\u6577\u5c45\u304c\u9ad8\u304f\u3001\u8ab0\u3067\u3082\u7c21\u5358\u306b\u624b\u304c\u51fa\u305b\u308b\u88c5\u7f6e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3057\u304b\u3057\u3001\u8fd1\u5e74\u306f\u5b9f\u9a13\u65b9\u6cd5\u304c\u5b9a\u7740\u3057\u3066\u304d\u305f\u3053\u3068\u3084\u65b0\u305f\u306a\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306e\u958b\u767a\u306a\u3069\u306b\u3088\u308a\u3001\u4ee5\u524d\u3088\u308a\u6271\u3044\u3084\u3059\u3044\u5206\u6790\u6a5f\u5668\u306b\u306a\u3063\u3066\u304d\u305f\u3068\u3044\u3048\u307e\u3059\u3002<\/p>\n

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\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<\/b>
\n\u30fbNGS\u89e3\u6790\u306b\u8208\u5473\u304c\u3042\u308b
\n\u30fbNGS\u306e\u5b9f\u9a13\u65b9\u6cd5\u306b\u3064\u3044\u3066\u77e5\u308a\u305f\u3044
\n\u30fb\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u3067\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u6295\u5165\u91cf\u306b\u60a9\u3093\u3067\u3044\u308b<\/p>\n

NGS\u89e3\u6790\u306e\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3068\u4eca\u56de\u306e\u691c\u8a3c\u5185\u5bb9<\/h2>\n

Ion Torrent\u2122\u30b7\u30b9\u30c6\u30e0\u3092\u306f\u3058\u3081\u3068\u3059\u308b\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u3067\u306e\u5b9f\u9a13\u306f\u3001\u4e00\u822c\u7684\u306b\u306f\u2460\u6838\u9178\u7cbe\u88fd\u3001\u2461\u76ee\u7684\u306eDNA\u3042\u308b\u3044\u306fRNA\u3092\u89e3\u6790\u53ef\u80fd\u306a\u72b6\u614b\u306b\u52a0\u5de5\u3059\u308b\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u8abf\u88fd\u3001\u2462\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u524d\u51e6\u7406\u306b\u3042\u305f\u308b\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u3001\u2463\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u3001\u3068\u3044\u30464\u3064\u306e\u30b9\u30c6\u30c3\u30d7\u306b\u5206\u304b\u308c\u3066\u3044\u307e\u3059\uff08\u56f31\uff09\u3002<\/p>\n

\"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\"<\/p>\n

\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u306b\u3064\u3044\u3066\u306f\u5404\u793e\u306e\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u3054\u3068\u306b\u624b\u6cd5\u304c\u7570\u306a\u308a\u3001Ion Torrent\u3067\u306f\u30a8\u30de\u30eb\u30b8\u30e7\u30f3PCR\u3068\u3044\u3046\u65b9\u6cd5\u3092\u63a1\u7528\u3057\u3066\u3044\u307e\u3059\u3002\u30a8\u30de\u30eb\u30b8\u30e7\u30f3PCR\u306f\u30aa\u30a4\u30eb\u4e2d\u306b\u5b58\u5728\u3059\u308b\u5404\u6c34\u6ef4\u5185\u306b1\u5206\u5b50\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30681\u500b\u306e\u30a2\u30c0\u30d7\u30bf\u30fc\u3068\u76f8\u88dc\u7684\u306aDNA\u304c\u7d50\u5408\u3057\u305f\u30d3\u30fc\u30ba\uff08ISP\uff1aIon Sphere Particle\uff09\u304c\u542b\u307e\u308c\u308b\u72b6\u614b\u3067PCR\u53cd\u5fdc\u3092\u884c\u3046\u3053\u3068\u306b\u3088\u308a\u3001ISP\u4e0a\u306b\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5897\u5e45\u3057\u307e\u3059\uff08\u56f32\uff09\u3002\u3053\u306e\u5de5\u7a0b\u306fIon Torrent\u88fd\u54c1\u3067\u306fIon OneTouch\u2122 2 System<\/a>\u3084Ion Chef\u2122 Instrument<\/a>\u3067\u884c\u308f\u308c\u307e\u3059\u3002\u30a8\u30de\u30eb\u30b8\u30e7\u30f3PCR\u306f\u30aa\u30a4\u30eb\u4e2d\u306b\u5b58\u5728\u3059\u308b\u5404\u6c34\u6ef4\u5185\u306b1\u5206\u5b50\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30681\u500b\u306eISP\uff08Ion Sphere Particle\uff09\u304c\u542b\u307e\u308c\u308b\u72b6\u614b\u3067PCR\u53cd\u5fdc\u3092\u884c\u3046\u3053\u3068\u306b\u3088\u308a\u3001ISP\u4e0a\u306b\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5897\u5e45\u3057\u307e\u3059\uff08\u56f32\uff09\u3002\u3053\u306e\u30a8\u30de\u30eb\u30b8\u30e7\u30f3PCR\u306b\u3088\u3063\u3066\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u5897\u5e45\u3055\u305b\u305fISP\u3092\u6700\u7d42\u7684\u306b\u534a\u5c0e\u4f53\u30c1\u30c3\u30d7\u306b\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u3057\u3001\u5404ISP\u306e\u5897\u5e45\u3057\u305fDNA\u914d\u5217\u3092\u89e3\u6790\u3059\u308b\u3053\u3068\u3067\u30b5\u30f3\u30d7\u30eb\u306e\u914d\u5217\u60c5\u5831\u3092\u53d6\u5f97\u3057\u307e\u3059\u3002<\/p>\n

\"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\"<\/p>\n

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\uff1c\u5408\u308f\u305b\u3066\u8aad\u307f\u305f\u3044\uff1a\u30b2\u30ce\u30e0\u89e3\u6790\u65b0\u6642\u4ee3\uff01 \u4eca\u3060\u304b\u3089\u3053\u305d\u805e\u304d\u305f\u3044\uff01Ion Torrent\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306e\u539f\u7406<\/a>\uff1e<\/p>\n

\u6750\u6599\u3068\u65b9\u6cd5 -Ion GeneStudio S5\u306b\u3088\u308b\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790-<\/h2>\n

\u4f7f\u7528\u3057\u305f\u6a5f\u5668<\/b>
\n\u30fb\u3000Ion S5\u2122 XL System<\/a> (A27214)
\n\u30fb\u3000Ion Chef\u2122 Instrument<\/a> (4484177)<\/p>\n

\u4f7f\u7528\u3057\u305f\u8a66\u85ac<\/b>
\n\u30fb\u3000Ion 540\u2122 Kit-Chef<\/a> (A30011)
\n\u30fb\u3000Ion 540\u2122 Chip Kit<\/a> (A27765)
\n\u30fb\u3000Ion S5\u2122 Controls Kit Plus<\/a> (A30729) (Human CEPH Control 200 Library\u3092\u4f7f\u7528)<\/p>\n

\u624b\u9806<\/b>
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\n\u30fb\u300050 pM\uff08\u63a8\u5968\u6fc3\u5ea6\uff09
\n\u30fb\u3000200 pM\uff08\u904e\u5270\u6fc3\u5ea6\uff09
\n\u30fb\u3000400 pM\uff08\u904e\u5270\u6fc3\u5ea6\uff09<\/p>\n

\u305d\u306e\u4ed6\u306e\u6761\u4ef6\u306b\u3064\u3044\u3066\u306fIon GeneStudio\u2122 S5\u3001Ion Chef\u2122\u304a\u3088\u3073Ion 540\u2122 Kit \u2013 Chef\u306e\u30e6\u30fc\u30b6\u30fc\u30ac\u30a4\u30c9\u306b\u5f93\u3044\u307e\u3057\u305f\u3002<\/p>\n

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\u65e9\u901f\u30b7\u30fc\u30b1\u30f3\u30b9\u89e3\u6790\u306e\u7d50\u679c\u3092\u898b\u3066\u3044\u304d\u307e\u3057\u3087\u3046\u3002\u307e\u305a\u3001\u534a\u5c0e\u4f53\u30c1\u30c3\u30d7\u306e\u30d2\u30fc\u30c8\u30de\u30c3\u30d7\uff08\u56f33\uff09\u306b\u7740\u76ee\u3059\u308b\u3068\u3001\u3044\u305a\u308c\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u6295\u5165\u91cf\u3067\u3082\u5168\u4f53\u304c\u8d64\u304f\u8868\u793a\u3055\u308c\u3066\u3044\u308b\u306e\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u3053\u308c\u306fISP\u304c\u534a\u5c0e\u4f53\u30c1\u30c3\u30d7\u5168\u4f53\u306b\u304f\u307e\u306a\u304f\u5145\u586b\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u3092\u793a\u3057\u3066\u304a\u308a\u3001\u30d2\u30fc\u30c8\u30de\u30c3\u30d7\u306e\u7d50\u679c\u3092\u898b\u308b\u9650\u308a\u306f\u3044\u305a\u308c\u306e\u6761\u4ef6\u3067\u3082\u554f\u984c\u306a\u304f\u30ed\u30fc\u30c7\u30a3\u30f3\u30b0\u3067\u304d\u3066\u3044\u308b\u3088\u3046\u306b\u898b\u3048\u307e\u3059\u3002<\/p>\n

\"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\"<\/p>\n

\u6b21\u306b\u305d\u308c\u305e\u308c\u306e\u89e3\u6790\u7d50\u679c\u306b\u3064\u3044\u3066\u30e9\u30f3\u30ec\u30dd\u30fc\u30c8\u306e\u8a73\u7d30\u3092\u898b\u3066\u3044\u304d\u307e\u3057\u3087\u3046\uff08\u56f34\uff09\u3002\u63a8\u5968\u6fc3\u5ea6\u3067\u3042\u308b50 pM\u3067\u306f\u89e3\u6790\u53ef\u80fd\u306a\u30ea\u30fc\u30c9\u6570\u3092\u793a\u3059Total Reads\u304c\u7d047,700\u4e07\u30ea\u30fc\u30c9\u3068\u306a\u3063\u3066\u304a\u308a\u30b9\u30da\u30c3\u30af\u3092\u5341\u5206\u306b\u6e80\u305f\u3057\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3067200 pM\u3067\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u3092\u884c\u3063\u305f\u7d50\u679c\u306b\u6ce8\u76ee\u3059\u308b\u3068\u3001\u53d6\u5f97\u30ea\u30fc\u30c9\u6570\u306f6,400\u4e07\u30ea\u30fc\u30c9\u3067\u3059\u306e\u3067Ion 540\u2122 Chip\u306e\u30b9\u30da\u30c3\u30af\u306f\u6e80\u305f\u3057\u3066\u3044\u307e\u3059\u304c\u300150 pM\u306e\u89e3\u6790\u7d50\u679c\u3068\u6bd4\u3079\u30661,000 \u4e07\u30ea\u30fc\u30c9\u4ee5\u4e0a\u3082Total Reads\u304c\u6e1b\u5c11\u3057\u3066\u3057\u307e\u3044\u307e\u3057\u305f\u3002\u5404\u6761\u4ef6\u3067\u306e\u7d50\u679c\u3092\u6bd4\u8f03\u3059\u308b\u3068\u3001200 pM\u3067\u306fISP Summary\u306eClonal\u5272\u5408\u304c68%\u304b\u308957%\u306b\u6e1b\u5c11\u3057\u3066\u3044\u308b\u306e\u304c\u308f\u304b\u308a\u307e\u3059\u3002\u53cd\u5bfe\u306bPolyclonal\u306e\u5272\u5408\u306f32%\u304b\u308943%\u306b\u5897\u52a0\u3057\u3066\u3044\u307e\u3059\u3002400 pM\u3067\u306fTotal Reads\u304c\u7d044,800\u4e07\u30ea\u30fc\u30c9\u3057\u304b\u53d6\u5f97\u3067\u304d\u3066\u304a\u3089\u305a\u3001Ion 540\u2122 Chip\u3067\u671f\u5f85\u3055\u308c\u308b\u30b9\u30da\u30c3\u30af\u3092\u6e80\u305f\u3057\u3066\u3044\u307e\u305b\u3093\u3067\u3057\u305f\u3002Clonal\u3068Polyclonal\u306e\u5272\u5408\u306b\u3064\u3044\u3066\u3082\u3001\u305d\u308c\u305e\u308c49%\u306851%\u3068\u306a\u3063\u3066\u304a\u308a\u3001200 pM\u3088\u308a\u3082\u3088\u308aClonal\u306e\u5272\u5408\u304c\u6e1b\u5c11\u3057\u3001Polyclonal\u304c\u5897\u52a0\u3057\u3066\u3044\u308b\u306e\u304c\u308f\u304b\u308a\u307e\u3059\u3002<\/p>\n

\"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\"<\/p>\n

\u3053\u3053\u3067\u51fa\u3066\u304f\u308bClonal\u3068\u3044\u3046\u306e\u306f1\u7a2e\u985e\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u7531\u6765\u306eDNA\u914d\u5217\u304c\u5897\u5e45\u3057\u305fISP\u306e\u5272\u5408\u3092\u793a\u3057\u307e\u3059\u3002\u53cd\u5bfe\u306bPolyclonal\u306f\u8907\u6570\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306eDNA\u914d\u5217\u304c\u5897\u5e45\u3057\u3066\u3044\u308bISP\u306e\u5272\u5408\u3092\u8868\u3057\u3066\u3044\u307e\u3059\u3002\u3059\u306a\u308f\u3061\u4eca\u56de\u306e\u89e3\u6790\u7d50\u679c\u3067\u306f\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u6642\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u6295\u5165\u91cf\u3092\u5897\u3084\u3059\u3053\u3068\u3067\u8907\u6570\u306eDNA\u914d\u5217\u304c\u5897\u5e45\u3057\u305fISP\uff08Polyclonal\uff09\u304c\u5897\u52a0\u3057\u3001\u305d\u308c\u304c\u539f\u56e0\u3068\u306a\u3063\u3066\u53d6\u5f97\u30ea\u30fc\u30c9\u6570\u304c\u6e1b\u5c11\u3057\u305f\u3053\u3068\u3092\u793a\u5506\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u306a\u305c\u3053\u306e\u69d8\u306a\u73fe\u8c61\u304c\u8d77\u3053\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002
\n\u5b9f\u306f\u30a8\u30de\u30eb\u30b8\u30e7\u30f3PCR\u306e\u539f\u7406\u3068\u5bc6\u63a5\u306a\u95a2\u4fc2\u304c\u3042\u308a\u307e\u3059\u3002\u5192\u982d\u3067\u7d39\u4ecb\u3057\u305f\u3088\u3046\u306b\u30a8\u30de\u30eb\u30b8\u30e7\u30f3PCR\u306f\u30aa\u30a4\u30eb\u4e2d\u306e\u6c34\u6ef4\u5185\u306b1\u5206\u5b50\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u30681\u500b\u306eISP\u3092\u542b\u3080\u72b6\u614b\u3067PCR\u53cd\u5fdc\u3092\u884c\u3044\u3001ISP\u4e0a\u306bDNA\u3092\u5897\u5e45\u3059\u308b\u624b\u6cd5\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u3053\u306e\u6642\u306b2\u672c\u4ee5\u4e0a\u306eDNA\u65ad\u7247\u304c1\u3064\u306e\u6c34\u6ef4\u5185\u306b\u5165\u3063\u3066\u3057\u307e\u3046\u3068\u8907\u6570\u7a2e\u985e\u306e\u5869\u57fa\u914d\u5217\u304c\u540c\u4e00\u306eISP\u4e0a\u306b\u5897\u5e45\uff08Polyclonal\u5897\u5e45\uff09\u3057\u307e\u3059\uff08\u56f35\uff09\u3002<\/p>\n

\"\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\"<\/p>\n

\u3064\u307e\u308a\u3001\u4eca\u56de\u306e\u691c\u8a3c\u5b9f\u9a13\u306b\u304a\u3044\u3066\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u6295\u5165\u91cf\u3092\u904e\u5270\u306b\u3057\u305f\u6761\u4ef6\u3067\u306f\u6c34\u6ef4\u5185\u306b2\u672c\u4ee5\u4e0a\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u304c\u6df7\u5728\u3059\u308b\u78ba\u7387\u304c\u9ad8\u307e\u308a\u3001Polyclonal\u306e\u5272\u5408\u304c\u5897\u52a0\u3057\u3066\u3057\u307e\u3063\u305f\u3068\u8003\u3048\u3089\u308c\u307e\u3059\u3002Polyclonal\u3068\u306a\u3063\u305fISP\u304b\u3089\u306f\u8907\u6570\u306eDNA\u914d\u5217\u306e\u30b7\u30b0\u30ca\u30eb\u304c\u691c\u51fa\u3055\u308c\u3066\u3057\u307e\u3046\u305f\u3081\u6b63\u5e38\u306a\u89e3\u6790\u30c7\u30fc\u30bf\u3092\u53d6\u5f97\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u3053\u306e\u3088\u3046\u306a\u30ea\u30fc\u30c9\u306f\u89e3\u6790\u6642\u306b\u30d5\u30a3\u30eb\u30bf\u30fc\u306b\u3088\u3063\u3066\u9664\u5916\u3055\u308c\u308b\u305f\u3081\u3001\u53d6\u5f97\u30ea\u30fc\u30c9\u6570\u3092\u5897\u3084\u305d\u3046\u3068\u3057\u3066\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u6295\u5165\u91cf\u3092\u904e\u5270\u306b\u3057\u3066\u3082\u3001\u6b8b\u5ff5\u306a\u304c\u3089\u7dcf\u30ea\u30fc\u30c9\u6570\u306f\u5897\u3048\u308b\u3069\u3053\u308d\u304b\u6e1b\u5c11\u3057\u3066\u3057\u307e\u3046\u306e\u3067\u3059\u3002<\/p>\n

\u3044\u304b\u304c\u3067\u3057\u305f\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n

\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u3067\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u6295\u5165\u91cf\u306f\u5358\u306b\u591a\u304f\u3059\u308c\u3070\u826f\u3044\u3068\u3044\u3046\u8a33\u3067\u306f\u306a\u304f\u6700\u9069\u306a\u6fc3\u5ea6\u304c\u5b58\u5728\u3057\u307e\u3059\u3002\u3088\u3063\u3066\u6700\u3082\u52b9\u7387\u3088\u304f\u89e3\u6790\u7d50\u679c\u3092\u5f97\u308b\u305f\u3081\u306b\u306f\u30e6\u30fc\u30b6\u30fc\u30ac\u30a4\u30c9\u306b\u57fa\u3065\u3044\u305f\u9069\u5207\u306a\u6761\u4ef6\u3067\u5b9f\u9a13\u3092\u884c\u3046\u3053\u3068\u304c\u975e\u5e38\u306b\u91cd\u8981\u306b\u306a\u3063\u3066\u304d\u307e\u3059\u3002<\/p>\n

\u5b9f\u969b\u306e\u5b9f\u9a13\u3067\u306f\u3042\u3048\u3066\u63a8\u5968\u6fc3\u5ea6\u3092\u9038\u8131\u3057\u3066\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u3092\u884c\u3046\u3053\u3068\u306f\u5c11\u306a\u3044\u304b\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u4f5c\u88fd\u3057\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u5b9a\u91cf\u3092\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u524d\u306b\u6b63\u78ba\u306b\u884c\u3048\u3066\u3044\u306a\u304b\u3063\u305f\u5834\u5408\u3001\u4eca\u56de\u306e\u3088\u3046\u306a\u904e\u5270\u91cf\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3092\u6295\u5165\u3057\u305f\u5b9f\u9a13\u6761\u4ef6\u304c\u518d\u73fe\u3055\u308c\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u5f53\u793e\u3067\u306fIonTaqMan\u2122 Library quantification kit<\/a>\u3092\u4f7f\u7528\u3057\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u5b9a\u91cf\u3092\u63a8\u5968\u3057\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u88fd\u54c1\u3092\u4f7f\u7528\u3059\u308c\u3070\u3001\u6b63\u78ba\u306a\u5b9a\u91cf\u5024\u3092\u3082\u3068\u306b\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u3067\u306e\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u306e\u6295\u5165\u91cf\u3092\u6c7a\u5b9a\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08Ion Xpress\u2122 Barcode Adapters 1-16 Kit (4471250\uff09\u304a\u3088\u3073IonCode\u2122 Barcode Adapters 1-384 Kit (A29751)\u306e\u30a2\u30c0\u30d7\u30bf\u30fc\u3092\u4f7f\u7528\u3057\u305f\u30e9\u30a4\u30d6\u30e9\u30ea\u30fc\u3067\u4f7f\u7528\u53ef\u80fd\u3001Ion Xpress\u2122 RNA-Seq Barcode (4475485) \u306f\u975e\u5bfe\u5fdc\u3067\u3059\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044) \u3002<\/p>\n

\u307e\u305f\u4eca\u56de\u306f\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8\u8abf\u88fd\u306b\u3064\u3044\u3066\u306e\u3054\u7d39\u4ecb\u3060\u3051\u3067\u3057\u305f\u304c\u3001\u5b9f\u306fIon Torrent\u2122\u306e\u89e3\u6790\u306b\u306f\u5b9f\u9a13\u3092\u5b89\u5b9a\u7684\u306b\u6210\u529f\u3055\u305b\u308b\u305f\u3081\u306e\u30b3\u30c4\u304c\u4ed6\u306b\u3082\u3042\u308a\u307e\u3059\u3002\u305d\u3053\u3067\u5f53\u793e\u3067\u306f\u5b9f\u9a13\u306e\u624b\u6280\u3092\u8a73\u7d30\u306b\u304a\u4f1d\u3048\u3059\u308b\u6a5f\u4f1a\u3068\u3057\u3066\u3001\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0<\/a>\u3092\u958b\u50ac\u3057\u3066\u3044\u307e\u3059\u3002\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0\u306e\u4e2d\u3067\u306f\u4eca\u56de\u306e\u3088\u3046\u306a\u5b9f\u9a13\u306e\u30dd\u30a4\u30f3\u30c8\u3092\u5404\u5de5\u7a0b\u3067\u3054\u7d39\u4ecb\u3057\u3066\u3044\u304d\u307e\u3059\u306e\u3067\u3001\u3053\u308c\u304b\u3089\u65b0\u3057\u304fIon Torrent\u3092\u7528\u3044\u305f\u5b9f\u9a13\u3092\u59cb\u3081\u3089\u308c\u308b\u65b9\u3001\u3042\u308b\u3044\u306f\u666e\u6bb5Ion Torrent\u2122\u3092\u7d99\u7d9a\u3057\u3066\u4f7f\u7528\u3057\u3066\u3044\u308b\u3051\u308c\u3069\u3082\u6539\u3081\u3066\u624b\u6280\u3092\u78ba\u8a8d\u3057\u305f\u3044\u65b9\u306a\u3069\u3001\u3054\u8208\u5473\u3042\u308b\u65b9\u306f\u304a\u6c17\u8efd\u306b
\n\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8<\/a>\u307e\u3067\u3054\u76f8\u8ac7\u304f\u3060\u3055\u3044\uff01<\/p>\n

 <\/p>\n

\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b9 \u30c7\u30fc\u30bf\u89e3\u6790\u30aa\u30f3\u30e9\u30a4\u30f3\u500b\u5225\u76f8\u8ac7<\/h2>\n

IonTorrent\u30e6\u30fc\u30b6\u3067\u30c7\u30fc\u30bf\u89e3\u6790\u306b\u95a2\u3059\u308b\u5177\u4f53\u7684\u306a\u8ab2\u984c\u3092\u304a\u6301\u3061\u306e\u65b9\u306b\u306f\u3001\u7121\u6599\u306e\u500b\u5225\u76f8\u8ac7\u306e\u6642\u9593\u3092\u958b\u8a2d\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u8a73\u7d30\u3092\u898b\u308b<\/a><\/p>\n

 <\/p>\n

\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"

\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b5\u306f\u907a\u4f1d\u5b50\u3092\u7db2\u7f85\u7684\u304b\u3064\u591a\u69d8\u306a\u7528\u9014\uff08\u5909\u7570\u89e3\u6790\u3084\u767a\u73fe\u89e3\u6790\u306a\u3069\uff09\u3067\u89e3\u6790\u3067\u304d\u308b\u975e\u5e38\u306b\u5f37\u529b\u306a\u5206\u6790\u88c5\u7f6e\u3067\u3059\u3002\u4e00\u6614\u524d\u307e\u3067NGS\uff08Next Generation 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