{"id":12809,"date":"2022-10-20T09:25:35","date_gmt":"2022-10-20T00:25:35","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=12809"},"modified":"2025-10-21T10:32:18","modified_gmt":"2025-10-21T01:32:18","slug":"western-blot-protein-ts-1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-1\/","title":{"rendered":"\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c\uff11\u56de\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac"},"content":{"rendered":"\n<figure class=\"wp-block-image aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"761\" height=\"476\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/western.jpg\" alt=\"westernblotting_protein\" class=\"wp-image-12829\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/western.jpg 761w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/western-300x188.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/western-320x200.jpg 320w\" sizes=\"auto, (max-width: 761px) 100vw, 761px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306f\u3001\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u767a\u73fe\u3092\u78ba\u8a8d\u3059\u308b\u65b9\u6cd5\u3068\u3057\u3066\u3001\u591a\u304f\u306e\u7814\u7a76\u8005\u306b\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3060\u304b\u3089\u3053\u305d\u3001\u5e02\u5834\u306b\u306f\u305f\u304f\u3055\u3093\u306e\u8a66\u85ac\u3084\u88c5\u7f6e\u3067\u6ea2\u308c\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u88fd\u54c1\u9078\u3073\u306b\u8ff7\u3063\u3066\u3057\u307e\u3046\u4eba\u3082\u591a\u3044\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n\n\n\n<p>\u3054\u81ea\u8eab\u306e\u76ee\u7684\u306b\u5408\u3063\u305f\u8a66\u85ac\u306f\u3069\u308c\u306a\u306e\u304b\uff1f\u672c\u5f53\u306b\u305d\u306e\u9078\u629e\u3067\u3046\u307e\u304f\u3044\u304f\u306e\u304b\uff1f<strong>\u305d\u306e\u4e0d\u5b89\u3001\u3053\u306e\u30d6\u30ed\u30b0\u304c\u89e3\u6c7a\u3057\u307e\u3059\uff01<\/strong><\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac\u306e\u9078\u3073\u65b9\">\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac\u306e\u9078\u3073\u65b9<\/h2>\n\n\n\n<figure class=\"wp-block-image aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"798\" height=\"532\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/westernblot_protein.jpg\" alt=\"westernblot_protein2\" class=\"wp-image-12831\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/westernblot_protein.jpg 798w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/westernblot_protein-300x200.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/westernblot_protein-768x512.jpg 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/westernblot_protein-96x65.jpg 96w\" sizes=\"auto, (max-width: 798px) 100vw, 798px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p>\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac\u306b\u306f\u3001\u7a4f\u3084\u304b\u306a\u6761\u4ef6\u3067\u62bd\u51fa\u3059\u308b\u3082\u306e\u3001\u5f37\u3044\u754c\u9762\u6d3b\u6027\u5264\u3067\u62bd\u51fa\u3059\u308b\u3082\u306e\u3068\u3055\u307e\u3056\u307e\u3067\u3059\u3002\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u5834\u5408\u3001SDS-PAGE\u524d\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5909\u6027\u3055\u305b\u307e\u3059\u306e\u3067\u3001\u7a4f\u3084\u304b\u306a\u6761\u4ef6\u3067\u62bd\u51fa\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u8868\u306b\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3059\u3059\u3081\u306e\u62bd\u51fa\u8a66\u85ac\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<p>&nbsp;<\/p>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><th>\u62bd\u51fa\u65b9\u6cd5<\/th><th>\u8a66\u85ac\u540d<\/th><th>\u30e1\u30ea\u30c3\u30c8<\/th><th>\u6ce8\u610f\u70b9<\/th><\/tr><tr><td rowspan=\"2\">\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa<br>(Whole Cell)<\/td><td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/lc2676?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc<\/a><\/td><td>\u305d\u306e\u307e\u307eSDS-PAGE\u306e\u30b2\u30eb\u306b\u30a2\u30d7\u30e9\u30a4\u3067\u304d\u308b\u305f\u3081\u3001\u975e\u5e38\u306b\u7c21\u4fbf<\/td><td>\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u6e2c\u5b9a\u304c\u3067\u304d\u308b\u30ad\u30c3\u30c8\u304c\u9650\u3089\u308c\u308b<\/td><\/tr><tr><td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/89900?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">RIPA\u30d0\u30c3\u30d5\u30a1\u30fc<\/a><\/td><td>\u591a\u304f\u306e\u6587\u732e\u3067\u4f7f\u7528\u3055\u308c\u3066\u304a\u308a\u3001\u6bd4\u8f03\u7684\u9ad8\u3044\u62bd\u51fa\u52b9\u7387\u304c\u671f\u5f85\u3067\u304d\u308b<\/td><td>\u30e1\u30fc\u30ab\u30fc\u306b\u3088\u3063\u3066\u7d44\u6210\u304c\u7570\u306a\u308b<\/td><\/tr><tr><td rowspan=\"2\">\u5206\u753b\u62bd\u51fa<\/td><td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/78835?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">NE-PER Nuclear and Cytoplasmic Extraction Reagents<\/a><\/td><td>\u7d30\u80de\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u6838\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u3051\u3066\u62bd\u51fa\u3067\u304d\u308b<br>\u591a\u304f\u306e\u6587\u732e\u3067\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b<\/td><td>\u6838\u5185\u3067\u30b2\u30ce\u30e0\u306b\u5f37\u304f\u7d50\u5408\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u62bd\u51fa\u3067\u304d\u306a\u3044<\/td><\/tr><tr><td><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/89842?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Mem-PER Plus Membrane Protein Extraction Kit<\/a><\/td><td>\u7d30\u80de\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u3051\u3066\u62bd\u51fa\u3067\u304d\u308b<\/td><td>\u819c\u8cab\u901a\u56de\u6570\u304c\u591a\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u96e3\u3057\u3044\u3053\u3068\u304c\u3042\u308b<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n<p>\u203b\u305d\u306e\u4ed6\u306e\u5206\u753b\u62bd\u51fa\u30ad\u30c3\u30c8\u306f<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-biology\/protein-purification-isolation\/cell-lysis-fractionation\/subcellular-fractionation.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u3061\u3089<\/a>\u3002<\/p>\n\n\n\n<p><strong>Whole Cell\u304b\u3089\u306e\u62bd\u51fa\u306b\u306f\u3001SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u4fbf\u5229<\/strong><br>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u305f\u3081\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u306b\u306f\u3001<strong>SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u975e\u5e38\u306b\u4fbf\u5229<\/strong>\u3067\u3059\u3002\u3053\u308c\u3092\u4f7f\u3048\u3070\u3001<strong>SDS-PAGE\u524d\u306b\u30b5\u30f3\u30d7\u30eb\u3068SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u6df7\u305c\u308b\u30b9\u30c6\u30c3\u30d7\u304c\u7701\u7565<\/strong>\u3067\u304d\u308b\u305f\u3081\u3067\u3059\u3002<\/p>\n\n\n\n<p>SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u305d\u306e\u7d44\u6210\u304b\u3089\u3055\u307e\u3056\u307e\u306a\u30b5\u30f3\u30d7\u30eb\u306b\u304a\u3044\u3066\u9ad8\u3044\u62bd\u51fa\u52b9\u679c\u304c\u671f\u5f85\u3067\u304d\u307e\u3059\u304c\u3001\u7d44\u7e54\u3084\u7d30\u80de\u58c1\u3092\u6301\u3064\u30b5\u30f3\u30d7\u30eb\u306e\u5834\u5408\u306f\u3001\u6a5f\u68b0\u7684\u306a\u7834\u7815\u3068\u306e\u4f75\u7528\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p>\uff1cSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\uff08Leammli SDS Sample Buffer\uff09\u306e\u4e00\u822c\u7684\u306a\u7d44\u6210\uff1e<br>65 mM Tris-HCl, 10% glycerol, 2% SDS, 0.025% bromophenol blue<br>\u203b\u3053\u308c\u306b\u7d42\u6fc3\u5ea650 mM\u3068\u306a\u308b\u3088\u3046DTT\u3092\u6dfb\u52a0\u3057\u3066\u7528\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u306a\u304a\u3001SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u5185\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u30ad\u30c3\u30c8\u306b\u306f\u9650\u308a\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u4e0a\u8a18\u7d44\u6210\u3067\u3042\u308c\u3070Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/22662?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 660nm Protein Assay Kit<\/a>\u304c\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u203b<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/B0008\" target=\"_blank\" rel=\"noopener\">Invitrogen\u2122 Bolt\u2122 LDS Sample Buffer<\/a>\u3084<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/NP0007\" target=\"_blank\" rel=\"noopener\">NuPAGE\u2122 LDS Sample Buffer<\/a>\u306f\u3001\u4e2d\u306b\u542b\u307e\u308c\u3066\u3044\u308b\u8272\u7d20\u304c660 nm\u306b\u5438\u53ce\u304c\u3042\u308b\u305f\u3081\u3001<b><u>Pierce\u2122 660nm Protein Assay<\/u><\/b><u><b>\u304c\u4f7f\u7528\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044<\/b><\/u>\u3002<\/p>\n\n\n\n<p><strong>\u7d30\u80de\u8cea\u3068\u6838\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u3051\u3066\u62bd\u51fa\u3067\u304d\u308b<\/strong><br><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/78835 ?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">NE-PER Nuclear and Cytoplasmic Extraction Reagents<\/a><br>\u4f8b\u3048\u3070\u8ee2\u5199\u56e0\u5b50\u306a\u3069\u3092\u7814\u7a76\u3057\u3066\u3044\u308b\u5834\u5408\u3001SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u4f7f\u3046\u3068\u3001Whole Cell\u304b\u3089\u62bd\u51fa\u3059\u308b\u305f\u3081\u3001\u7d30\u80de\u8cea\u7531\u6765\u304b\u6838\u7531\u6765\u306a\u306e\u304b\u308f\u304b\u3089\u306a\u304f\u306a\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u3001\u7d30\u80de\u8cea\u3068\u6838\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u3051\u3066\u62bd\u51fa\u3057\u305f\u3044\u5834\u5408\u306f\u3001Thermo Scientific\u2122 NE-PER\u2122 Nuclear and Cytoplasmic Extraction Reagents\uff08\u4ee5\u964dNE-PER Reagents\uff09\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002\u4eca\u307e\u30671000\u5831\u8fd1\u3044\u6587\u732e\u3067\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u6b74\u53f2\u3068\u5b9f\u7e3e\u3092\u517c\u306d\u5099\u3048\u305f\u8a66\u85ac\u3067\u3059\u3002<\/p>\n\n\n\n<p>NE-PER Reagents\u3067\u62bd\u51fa\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u306f\u3001Thermo Scientific\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/23225 ?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 BCA Assay Kit<\/a>\u3084<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/22662 ?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Pierce\u2122 660nm Protein Assay Kit<\/a>\u306a\u3069\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u306e\u6e2c\u5b9a\u304c\u3067\u304d\u307e\u3059\u3002\u62bd\u51fa\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3092\u76f4\u63a5SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u6df7\u305c\u3066SDS-PAGE\u3082\u3067\u304d\u307e\u3059\u304c\u3001\u6838\u753b\u5206\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u5869\u6fc3\u5ea6\u304c\u9ad8\u3044\u305f\u3081\u30014\u500d\u4ee5\u4e0a\u306b\u8584\u3081\u308b\u304b\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u4ea4\u63db\u3092\u3057\u3066\u304b\u3089\u30a2\u30d7\u30e9\u30a4\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<p><strong>\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u89e3\u6790\u306b<\/strong><br><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/89842 ?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Mem-PER Plus Membrane Protein Extraction Kit<\/a><br>\u30b7\u30b0\u30ca\u30eb\u4f1d\u9054\u3084\u7d30\u80de\u5185\u3078\u306e\u8f38\u9001\u30e1\u30ab\u30cb\u30ba\u30e0\u306e\u89e3\u660e\u306a\u3069\u3001\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7814\u7a76\u306f\u4eca\u3067\u3082\u3055\u304b\u3093\u306b\u884c\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306b\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u62bd\u51fa\u3057\u305f\u3044\u5834\u5408\u306b\u6d3b\u8e8d\u3059\u308b\u306e\u304cThermo Scientific\u2122 Mem-PERTM Plus Membrane Protein Extraction Kit\uff08\u4ee5\u964dMem-PER Plus Kit\uff09\u3067\u3059\u3002\u7d30\u80de\u8cea\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3001\u819c\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u3051\u3066\u62bd\u51fa\u304c\u53ef\u80fd\u3067\u3059\u3002\u62bd\u51fa\u3057\u305f\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u6fc3\u5ea6\u6e2c\u5b9a\u5f8c\u3001\u7279\u306b\u8131\u5869\u3084\u900f\u6790\u306a\u3069\u5fc5\u8981\u3068\u305b\u305a\u3001SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3068\u6df7\u305c\u308b\u3060\u3051\u3067SDS-PAGE\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u306a\u304a\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u62bd\u51fa\u3057\u305f\u5f8c\u3001\u7c98\u6027\u304c\u9ad8\u3044\u3068\u304d\u306f\u30b5\u30f3\u30d7\u30eb\u4e2d\u306b\u30b2\u30ce\u30e0DNA\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u53ef\u80fd\u6027\u304c\u9ad8\u3044\u3067\u3059\u3002\u30bd\u30cb\u30b1\u30fc\u30bf\u30fc\u3092\u7528\u3044\u3066\u5206\u89e3\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u963b\u5bb3\u65b9\u6cd5\u306e\u9078\u3073\u65b9\">\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u963b\u5bb3\u65b9\u6cd5\u306e\u9078\u3073\u65b9<\/h2>\n\n\n\n<figure class=\"wp-block-image aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"504\" height=\"235\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/WesternBlot_protein3.jpg\" alt=\"WesternBlot_protein3\" class=\"wp-image-12822\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/WesternBlot_protein3.jpg 504w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2022\/10\/WesternBlot_protein3-300x140.jpg 300w\" sizes=\"auto, (max-width: 504px) 100vw, 504px\" \/><\/figure>\n\n\n\n<p><br>\u62bd\u51fa\u306e\u3068\u304d\u306b\u6c17\u3092\u4ed8\u3051\u306a\u3051\u308c\u3070\u3044\u3051\u306a\u3044\u306e\u304c\u3001\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306e\u5b58\u5728\u3067\u3059\u3002\u76ee\u7684\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u4e00\u7dd2\u306b\u5185\u5728\u6027\u306e\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u3082\u62bd\u51fa\u3055\u308c\u307e\u3059\u306e\u3067\u3001\u5927\u5207\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5206\u89e3\u3055\u308c\u306a\u3044\u3088\u3046\u306b\u5b88\u3063\u3066\u3042\u3052\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<p><strong>\u30fb\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u963b\u5bb3\u5264\u3092\u7528\u3044\u308b<\/strong><br>\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u306e\u3068\u304d\u306f\u3001\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u963b\u5bb3\u5264\u3092\u7528\u3044\u307e\u3057\u3087\u3046\u3002\u3088\u304f\u77e5\u3089\u308c\u305f\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u963b\u5bb3\u5264\u3060\u3051\u3067\u3082AEBSF\u3001Leupeptin\u3001Bestain\u306a\u3069\u3001\u305f\u304f\u3055\u3093\u306e\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3001<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/protein-biology\/protein-purification-isolation\/cell-lysis-fractionation\/protease-phosphatase-inhibition\/protease-inhibitors.html?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30ab\u30af\u30c6\u30eb\u30bf\u30a4\u30d7<\/a>\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u3002<\/p>\n\n\n\n<p><strong>\u30fb\u5909\u6027\u5264\u3092\u7528\u3044\u308b<\/strong><br>\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u81ea\u8eab\u3082\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3059\u306e\u3067\u3001\u62bd\u51fa\u8a66\u85ac\u306bSDS\u3084\u30b0\u30a2\u30cb\u30b8\u30f3\u5869\u9178\u306a\u3069\u306e\u5f37\u529b\u306a\u5909\u6027\u5264\u304c\u5165\u3063\u3066\u3044\u308b\u5834\u5408\u306f\u3001\u3053\u308c\u3089\u304c\u591a\u304f\u306e\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u3092\u5909\u6027\u3055\u305b\u3001\u305d\u306e\u50cd\u304d\u3092\u6291\u3048\u8fbc\u307f\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u62bd\u51fa\u3059\u308b\u969b\u306f\u5fc5\u9808\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u5b8c\u5168\u306b\u5168\u3066\u306e\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u304c\u5931\u6d3b\u3067\u304d\u308b\u308f\u3051\u3067\u306f\u306a\u3044\u306e\u3067\u3001\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u963b\u5bb3\u5264\u3092\u5165\u308c\u305f\u65b9\u304c\u3044\u3044\u3067\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<p><strong>\u30fb4 \u2103\u3067\u62bd\u51fa\u3092\u884c\u3046<\/strong><br>\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u306f37 \u2103\u3067\u6700\u3082\u6d3b\u6027\u5316\u3055\u308c\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u62bd\u51fa\u4f5c\u696d\u306e\u9593\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u4f4e\u6e29\u306b\u4fdd\u3064\u3053\u3068\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5909\u6027\u3084\u5206\u89e3\u3092\u9632\u3050\u306e\u306b\u52b9\u679c\u7684\u3067\u3059\u3002\u3057\u304b\u3057\u4f4e\u6e29\u3067\u6d3b\u6027\u3092\u6301\u3064\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u3082\u5b58\u5728\u3059\u308b\u305f\u3081\u3001\u3053\u308c\u3060\u3051\u3067\u306f\u4e0d\u5341\u5206\u306a\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002\u30d7\u30ed\u30c6\u30a2\u30fc\u30bc\u963b\u5bb3\u5264\u3092\u5fc5\u305a\u4f75\u7528\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u307e\u3068\u3081-\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac\u306e\u9078\u3073\u65b9\">\u307e\u3068\u3081\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac\u306e\u9078\u3073\u65b9<\/h2>\n\n\n\n<p>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u305f\u3081\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u306f\u3001\u5909\u6027\u3092\u6c17\u306b\u3059\u308b\u5fc5\u8981\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001Whole\u3000Cell\u304b\u3089\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u62bd\u51fa\u3059\u308b\u5834\u5408\u306f\u3001\u3059\u3050\u306bSDS-PAGE\u304c\u3067\u304d\u308bSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u4fbf\u5229\u3067\u3059\u3002\u5206\u753b\u62bd\u51fa\u3092\u3059\u308b\u5834\u5408\u306f\u3001NE-PER Reagents\u3084Mem-PER Plus Kit\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-2\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u6b21\u56de<\/a>\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u62bd\u51fa\u3057\u305f\u5f8c\u306e\u3001\u300c\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6fc3\u5ea6\u6e2c\u5b9a\u65b9\u6cd5\u306e\u9078\u3073\u65b9\u300d\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<h4 class=\"wp-block-heading\" id=\"h-\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u30b7\u30ea\u30fc\u30ba\u306f\u3053\u3061\u3089\">\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u30b7\u30ea\u30fc\u30ba\u306f\u3053\u3061\u3089\uff1a<\/h4>\n\n\n\n<ul class=\"wp-block-list\">\n<li>\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c1\u56de\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa\u65b9\u6cd5<\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-2\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c2\u56de\uff1a\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-3\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">SDS-PAGE\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c3\u56de<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-4\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u8ee2\u5199\u6761\u4ef6\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c4\u56de<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-5\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c5\u56de<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-6\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" 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rel=\"noopener\">\u3088\u308a\u77ed\u6642\u9593\u3067\u3001\u3088\u308a\u7c21\u5358\u306b\u3002\u9ad8\u901f\u30bf\u30f3\u30d1\u30af\u8cea\u8ee2\u5199\u88c5\u7f6e\u304c\u751f\u307e\u308c\u305f\u30ef\u30b1<\/a><\/li>\n\n\n\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/westernblotting_ibind_bid_ts_1\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u81ea\u52d5\u5316\u3068\u306e\u95d8\u3044\uff5e\u6bdb\u7d30\u7ba1\u73fe\u8c61\u304c\u8d77\u3053\u3057\u305f\u9769\u547d\u3068\u306f\uff1f<\/a><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/chemiluminescent-westernblotting-guide\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3053\u308c\u304c\u3042\u308c\u3070\u3082\u3046\u30ab\u30f3\u30da\u30ad\uff01\u5316\u5b66\u767a\u5149\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u5b8c\u5168\u30ac\u30a4\u30c9<\/a><\/li>\n<\/ul>\n<\/div>\n\n\n\n<div style=\"height:10px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<div class=\"wp-block-group is-vertical is-layout-flex wp-container-core-group-is-layout-8cf370e7 wp-block-group-is-layout-flex\">\n<h4 class=\"wp-block-heading has-medium-font-size\" 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noreferrer\">\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n<\/div>\n<\/div>\n\n\n\n<div style=\"height:20px\" aria-hidden=\"true\" class=\"wp-block-spacer\"><\/div>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u95a2\u9023-\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089\">\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u95a2\u9023\u3001\u305d\u306e\u4ed6\u306e\u8a18\u4e8b\u306f\u3053\u3061\u3089<\/h3>\n\n\n\n<figure class=\"wp-block-table\"><table class=\"has-fixed-layout\"><tbody><tr><td>\n<ul>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic6\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c6\u56de\u3011\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3051\u308b\u30b9\u30c8\u30ea\u30c3\u30d4\u30f3\u30b0\u6cd5\u3068\u30ea\u30d7\u30ed\u30fc\u30d3\u30f3\u30b0\u6cd5<br><\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic7\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c7\u56de\u3011\u3044\u307e\u3055\u3089\u805e\u3051\u306a\u3044\u300c\u30d5\u30a1\u30fc\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u6cd5\u300d\u8d85\u5165\u9580<br><\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic15\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u7b2c15\u56de\u3011\u521d\u5fc3\u8005\u5fc5\u898b\uff01\u86cd\u5149\u30d7\u30ed\u30fc\u30d6\u306e\u57fa\u790e<br><\/a><\/li>\n<li><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-basic16\/?CID=bid_pca_wwg_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_western-blot-protein-ts-1_Social_LAB\" target=\"_blank\" 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