{"id":1288,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_38_2_alexafluor\/"},"modified":"2023-12-28T14:03:48","modified_gmt":"2023-12-28T05:03:48","slug":"user_voice_38_2_alexafluor","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_38_2_alexafluor\/","title":{"rendered":"\u819c\u30bf\u30f3\u30d1\u30af\u8ceaATP1B1\u306e\u5c40\u5728\u3092\u30c1\u30e9\u30df\u30c9\u30b7\u30b0\u30ca\u30eb\u5897\u5e45\u6280\u8853\u3067\u898b\u4e8b\u306b\u30ad\u30e3\u30c3\u30c1\uff08\u5317\u6d77\u9053\u5927\u5b66\u5927\u5b66\u9662\u533b\u5b66\u7814\u7a76\u79d1 \u5927\u5834\u96c4\u4ecb\u6c0f\uff09-\u300cNEXT\u300d2016\u5e7412\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2><span style=\"font-size: 12pt\">\u819c\u30bf\u30f3\u30d1\u30af\u8ceaATP1B1\u306e\u5c40\u5728\u3092\u30c1\u30e9\u30df\u30c9\u30b7\u30b0\u30ca\u30eb\u5897\u5e45\u6280\u8853\u3067\u898b\u4e8b\u306b\u30ad\u30e3\u30c3\u30c1<\/span><\/h2>\n<p><strong>\u5927\u5834\u96c4\u4ecb \u6c0f\uff08\u5317\u6d77\u9053\u5927\u5b66\u5927\u5b66\u9662\u533b\u5b66\u7814\u7a76\u79d1\u7d30\u80de\u751f\u7406\u5b66\u5206\u91ce\u6559\u6388\uff09<\/strong><br \/>\n<strong>\u85e4\u5ca1\u5bb9\u4e00\u6717 \u6c0f\uff08\u52a9\u6559\uff09\uff0f\u4f50\u85e4\u7d62 \u6c0f\uff08\u5927\u5b66\u9662\u751f\uff09<\/strong><\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/DSC08192.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3737 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/DSC08192.jpg\" alt=\"\u5927\u5834\u96c4\u4ecb 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P 1 B 1\uff08ATPase, Na+\/K+ transporting subunit beta 1\uff09\u306e\u6319\u52d5\u3092\u89b3\u5bdf\u3059\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u300c\u3068\u3053\u308d\u304c\u3053\u308c\u307e\u3067\u7814\u7a76\u5ba4\u3067\u554f\u984c\u306a\u304f\u4f7f\u3063\u3066\u304d\u305f\u6a19\u6e96\u7684\u306aAlexa\u4e8c\u6b21\u6297\u4f53\u3067\u306f\u307b\u3068\u3093\u3069\u30b7\u30b0\u30ca\u30eb\u304c\u89b3\u5bdf\u3067\u304d\u305a\u306b\u56f0\u3063\u3066\u3044\u307e\u3057\u305f\u300d\u3068\u52a9\u6559\u306e\u85e4\u5ca1\u5bb9\u4e00\u6717\u6c0f\u306f\u8a9e\u308a\u307e\u3059\u3002\u300c\u3061\u3087\u3046\u3069\u53d7\u3051\u53d6\u3063\u305f\u30e1\u30fc\u30eb\u30de\u30ac\u30b8\u30f3\u3067\u3001\u30c1\u30e9\u30df\u30c9\u30b7\u30b0\u30ca\u30eb\u5897\u5e45\u6cd5\u3092\u57fa\u76e4\u306b\u3057\u305fAlexa Fluor Tyramide SuperBoost 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href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/c09d871e60d59fc9c534a34bd7786799.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3738 size-medium\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/c09d871e60d59fc9c534a34bd7786799-300x98.jpg\" alt=\"[\u5185\u5728\u6027ATP1B1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u86cd\u5149\u67d3\u8272]\" width=\"300\" height=\"98\" \/><\/a><span style=\"font-size: 9pt\"><strong>[\u5185\u5728\u6027ATP1B1\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u86cd\u5149\u67d3\u8272]<\/strong><\/span><br \/>\n<span style=\"font-size: 9pt\">HeLa\u7d30\u80de\u3092\u56fa\u5b9a\u30fb\u900f\u904e\u3057\u3001\u6297ATP1B1\u6297\u4f53\uff08\u30de\u30a6\u30b9\uff09\u3068\u53cd\u5fdc\u5f8c\u3001A\u306f\u6a19\u6e96\u7684\u306aAlexa Fluora\u4e8c\u6b21\u6297\u4f53\u3001B\u306fAlexa Fluor 488 Tyramide SuperBoost \u30ad\u30c3\u30c8\uff08\u6297\u30de\u30a6\u30b9\u6297\u4f53\u3001\u30e4\u30ae\uff09\u3067\u67d3\u8272\u3002C\u306f\u3001\u6838\u67d3\u8272\uff08Hoechst33342,\u9752\uff09\u3068B\u306e\u67d3\u8272\uff08\u7dd1\uff09\u3068\u306e\u91cd\u306d\u5408\u308f\u305b\u753b\u50cf\u3002SuperBoost\u30ad\u30c3\u30c8\u3092\u4f7f\u3046\u3053\u3068\u3067\u3001\u819c\u306b\u5c40\u5728\u3059\u308bATP1B1\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u660e\u77ad\u306b\u89b3\u5bdf\u3067\u304d\u307e\u3057\u305f\u3002<\/span><\/p>\n<h2><span style=\"font-size: 12pt\">\u5c11\u91cf\u306e\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u9ad8\u5206\u89e3\u80fd\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u3092\u5b9f\u73fe!<br \/>\nAlexa Fluor Tyramide SuperBoost Kit<\/span><\/h2>\n<p><a href=\"http:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-analysis\/cellular-imaging\/immunofluorescence\/tyramide-signal-amplification-tsa.html?CID=fl-superboost?CID=bid_pca_aus_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab3n_Social_LAB\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignnone wp-image-3734 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/23275QuantitativeClrmetricPeptide-650x600.jpg\" alt=\"Alexa Fluor Tyramide SuperBoost Kit\" width=\"250\" height=\"204\" \/><\/a><\/p>\n<p>Invitrogen\u2122 Alexa Fluor\u2122 Tyramide SuperBoost\u2122 Kit\u306f\u3001\u30dd\u30ea-HRP\u3092\u4ecb\u3059\u308b\u30c1\u30e9\u30df\u30c9\u30b7\u30b0\u30ca\u30eb\u5897\u5e45\u6cd5\u306b\u3001Alexa Fluor\u8272\u7d20\u306e\u9ad8\u8f1d\u5ea6\u6027\u80fd\u3092\u7d44\u307f\u5408\u308f\u305b\u308b\u3053\u3068\u3067\u3001\u6a19\u6e96\u7684\u306a\u514d\u75ab\u67d3\u8272\u3088\u308a\u308210-200\u500d\u3082\u9ad8\u3044\u611f\u5ea6\u3092\u5b9f\u73fe\u3057\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u8d85\u9ad8\u611f\u5ea6 \uff1a \u691c\u51fa\u56f0\u96e3\u306a\u4f4e\u542b\u91cf\u30bf\u30fc\u30b2\u30c3\u30c8\u3082\u691c\u51fa\u53ef\u80fd<\/li>\n<li>\u30b7\u30f3\u30d7\u30eb\u306a\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3067\u3001\u6a19\u6e96\u30d5\u30a3\u30eb\u30bf\u30fc\u5bfe\u5fdc\u306e\u30b7\u30b0\u30ca\u30eb\u3092\u5897\u5e45\u3002\u7d30\u80de\u67d3\u8272\u3001\u7d44\u7e54\u67d3\u8272\u3001FISH\u6cd5\u306a\u3069\u306e\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u306b\u3001\u5f93\u6765\u6cd5\u3068\u307b\u307c\u540c\u3058\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u3067\u5bfe\u5fdc\u3002<\/li>\n<li>\u30de\u30eb\u30c1\u30ab\u30e9\u30fc\u30a4\u30e1\u30fc\u30b8\u30f3\u30b0\u306b\u5bfe\u5fdc\u3002DAPI\u3084\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\uff08GFP\u3084RFP\u306a\u3069\uff09\u3068\u4f75\u7528\u53ef\u80fd\u3002<\/li>\n<\/ul>\n<p><a href=\"http:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cell-analysis\/cellular-imaging\/immunofluorescence\/tyramide-signal-amplification-tsa.html?cid=fl-superboost?CID=bid_pca_aus_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab3n_Social_LAB\" target=\"_blank\" rel=\"noopener\">Alexa Fluor Tyramide SuperBoost Kit\u306e\u88fd\u54c1\u4e00\u89a7\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n<h2><span style=\"font-size: 12pt\">\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=bid_pca_aus_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab3n_Social_LAB\" target=\"_blank\" rel=\"noopener\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-3647\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/foregroundimg.img_.full_.high_-4-3.gif\" alt=\"NEXT2016\u5e7412\u6708\u53f7\" width=\"167\" height=\"235\" \/><\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2016\u5e7412\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=bid_pca_aus_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_ab3n_Social_LAB\" target=\"_blank\" 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