{"id":1297,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_38_3_cas9\/"},"modified":"2023-12-27T17:58:24","modified_gmt":"2023-12-27T08:58:24","slug":"user_voice_38_3_cas9","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_38_3_cas9\/","title":{"rendered":"\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30fc\u30e2\u30bb\u30c3\u30c8\u3067\u8133\u306e\u9ad8\u6b21\u6a5f\u80fd\u89e3\u6790\u3092\u52a0\u901f\uff08\u5b9f\u9a13\u52d5\u7269\u4e2d\u592e\u7814\u7a76\u6240 \u30de\u30fc\u30e2\u30bb\u30c3\u30c8\u7814\u7a76\u90e8 \u4f50\u3005\u6728\u3048\u308a\u304b\u6c0f\uff09-\u300cNEXT\u300d2016\u5e7412\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"

Cas9\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5c0e\u5165\u3067\u3001\u30e2\u30b6\u30a4\u30af\u306a\u3057\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u76ee\u6307\u3059
\n<\/span><\/h2>\n

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\n<\/strong><\/p>\n

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1 Generation of transgenic non-human primates with germline transmission \/ Sasaki E. et.al Nature.\u00a0459:523-7\uff082009\uff09<\/span>
\n2 Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient\u00a0Genome Editing \/ Sato K. et. al Cell Stem Cell 19:127-138\uff082016\uff09<\/span><\/p>\n

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\u6570\u5e74\u524d\u306e\u56fd\u969b\u5b66\u4f1a\u3067\u306f\u3042\u307e\u308a\u5927\u304d\u306a\u53cd\u5fdc\u306f\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001\u4eca\u5e74\u306e\u8b1b\u6f14\u5f8c\u306b\u306f\u8cea\u554f\u304c\u6bba\u5230\u3057\u3001\u5171\u540c\u7814\u7a76\u306e\u58f0\u3082\u304b\u304b\u308a\u307e\u3057\u305f\u3002\u4e16\u754c\u4e2d\u304b\u3089\u3001\u30de\u30fc\u30e2\u30bb\u30c3\u30c8\u3092\u4f7f\u3063\u305f\u7814\u7a76\u6210\u679c\u304c\u5831\u544a\u3055\u308c\u308b\u65e5\u306f\u76ee\u524d\u306e\u3088\u3046\u3067\u3059\u3002<\/p>\n

\u5199\u771f\u306f\u5b9f\u9a13\u52d5\u7269\u4e2d\u592e\u7814\u7a76\u6240\u3067\u98fc\u80b2\u4e2d\u306e\u30de\u30fc\u30e2\u30bb\u30c3\u30c8\uff08\u5199\u771f\u63d0\u4f9b\uff1a\u4f50\u3005\u6728\u3048\u308a\u304b\u6c0f\uff09<\/span><\/p>\n

\u6700\u5927\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u3001\u6700\u5c0f\u9650\u306e\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u5207\u65ad\u3092\u5b9f\u73fe
\nGeneArt Platinum Cas9 Nuclease\uff08\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u4ed8\u304d\uff09<\/span><\/h2>\n

\"GeneArt<\/a>Invitrogen\u2122 GeneArt\u2122 Platinum Cas9 Nuclease\u306f\u3001\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u4ed8\u304d\u306e\u91ce\u751f\u578bCas9\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308a\u3001CRISPR-Cas9\u30b7\u30b9\u30c6\u30e0\u3067\u4f7f\u7528\u3059\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u30c4\u30fc\u30eb\u3067\u3059\u3002\u30ac\u30a4\u30c9RNA\uff08gRNA\uff09\u3068\u5b89\u5b9a\u3057\u305f\u30ea\u30dc\u30bf\u30f3\u30d1\u30af\u8cea\uff08RNP\uff09\u8907\u5408\u4f53\u3092\u5f62\u6210\u5f8c\u3001\u7d30\u80de\u3078\u5c0e\u5165\u3057\u307e\u3059\u3002DNA\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u306e CRISPRCas9\u30d9\u30af\u30bf\u30fc\u3084mRNA\u30b7\u30b9\u30c6\u30e0\u3088\u308a\u3082\u306f\u308b\u304b\u306b\u9ad8\u3044\u7de8\u96c6\u52b9\u7387\u3092\u5b9f\u73fe\u3057\u3001\u7d30\u80de\u304b\u3089\u6025\u901f\u306b\u9664\u53bb\u3055\u308c\u308b\u305f\u3081\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u5207\u65ad\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u307e\u3059\u3002<\/p>\n

GeneArt Platinum Cas9 Nuclease\u306e\u8a73\u7d30\u60c5\u5831\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n

\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n

\"NEXT2016\u5e7412\u6708\u53f7\"<\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2016\u5e7412\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002
\nNEXT\u306e\u30d0\u30c3\u30af\u30ca\u30f3\u30d0\u30fc\u306f\u3053\u3061\u3089<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

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