{"id":1311,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_39_1_cas9\/"},"modified":"2023-12-27T17:58:26","modified_gmt":"2023-12-27T08:58:26","slug":"user_voice_39_1_cas9","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_39_1_cas9\/","title":{"rendered":"CRISPR\u3067\u9577\u3044DNA\u65ad\u7247\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u500b\u4f53\u3082\u78ba\u5b9f\u306b\u4f5c\u88fd\uff08\u4eac\u90fd\u5927\u5b66\u30a6\u30a4\u30eb\u30b9\u30fb\u518d\u751f\u533b\u79d1\u5b66\u7814\u7a76\u6240 \u8fd1\u85e4\u7384\u6c0f\u3001\u6e21\u908a\u4ec1\u7f8e\u6c0f\uff09\u3000-\u300cNEXT\u300d2017\u5e743\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"

\u6bd2\u6027\u306e\u4f4e\u3044GeneArt Platinum Cas9 Nuclease\u3067\u52b9\u7387\u3092\u5411\u4e0a
\n<\/span><\/h2>\n

\u8fd1\u85e4 \u7384 \u6c0f\uff08\u4eac\u90fd\u5927\u5b66\u30a6\u30a4\u30eb\u30b9\u30fb\u518d\u751f\u533b\u79d1\u5b66\u7814\u7a76\u6240 \u7d71\u5408\u751f\u4f53\u30d7\u30ed\u30bb\u30b9\u5206\u91ce\u30fb\u6559\u6388\uff0f\u9644\u5c5e\u518d\u751f\u5b9f\u9a13\u52d5\u7269\u65bd\u8a2d\u30fb\u65bd\u8a2d\u9577\uff0f\u4eac\u90fd\u5927\u5b66\u5927\u5b66\u9662\u533b\u5b66\u7814\u7a76\u79d1\u30fb\u907a\u4f1d\u5b50\u6539\u5909\u52d5\u7269\u5b66\u30fb\u6559\u6388\uff09\u3000<\/strong>
\n\u6e21\u908a\u4ec1\u7f8e \u6c0f\uff08\u4eac\u90fd\u5927\u5b66\u30a6\u30a4\u30eb\u30b9\u30fb\u518d\u751f\u533b\u79d1\u5b66\u7814\u7a76\u6240 \u9644\u5c5e\u518d\u751f\u5b9f\u9a13\u52d5\u7269\u65bd\u8a2d\u30fb\u52a9\u6559\uff09<\/strong><\/p>\n

\"\u8fd1\u85e4<\/a>\u3053\u306e1\u5e74\u3067\u6210\u529f\u3057\u305f\u30de\u30a6\u30b9\u500b\u4f53\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u306f20\u4f8b\u306b\u4e0a\u308b\u3068\u3044\u3046\u8fd1\u85e4\u7384\u6c0f\u3002\u4eac\u90fd\u5927\u5b66\u30a6\u30a4\u30eb\u30b9\u30fb\u518d\u751f\u533b\u79d1\u5b66\u7814\u7a76\u6240\u9644\u5c5e\u518d\u751f\u5b9f\u9a13\u52d5\u7269\u65bd\u8a2d\u306e\u65bd\u8a2d\u9577\u3068\u3057\u3066\u3001\u5b66\u5916\u304b\u3089\u3082\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u4f5c\u88fd\u306e\u76f8\u8ac7\u3092\u53d7\u3051\u3066\u3044\u307e\u3059\u3002\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u4f5c\u88fd\u6b7430\u5e74\u3067\u84c4\u3048\u305f\u57fa\u76e4\u6280\u8853\u3092\u652f\u3048\u306b\u3001CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u3092\u52b9\u7387\u7684\u306b\u4f7f\u3044\u3053\u306a\u3057\u307e\u3059\u3002\u8fd1\u85e4\u6c0f\u3068\u5171\u306b\u5b9f\u9a13\u3092\u9032\u3081\u308b\u6e21\u908a\u4ec1\u7f8e\u6c0f\u306b\u3082\u6280\u8853\u6539\u5584\u306e\u30dd\u30a4\u30f3\u30c8\u3092\u4f3a\u3044\u307e\u3057\u305f\u3002<\/p>\n

\u30ce\u30c3\u30af\u30a4\u30f3\u52b9\u7387\u3092\u7740\u5b9f\u306b\u5411\u4e0a<\/span><\/h2>\n

2\u5e74\u524d\u306bCRISPR\/Cas9\u6280\u8853\u306e\u5c0e\u5165\u306b\u8e0f\u307f\u5207\u3063\u305f\u3068\u3044\u3046\u8fd1\u85e4\u6c0f\u3002\u300c\u7acb\u3061\u4e0a\u3052\u306b\u82e6\u52b4\u3059\u308b\u4f8b\u3082\u805e\u304d\u307e\u3059\u304c\u3001\u53d7\u7cbe\u5375\u3078\u306e\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u3084\u5b50\u5bae\u3078\u306e\u79fb\u690d\u3001\u30de\u30a6\u30b9\u306e\u98fc\u80b2\u3084\u30e2\u30b6\u30a4\u30af\u500b\u4f53\u304b\u3089\u4f53\u5916\u53d7\u7cbe\u3092\u7d4c\u3066\u30db\u30e2\u306e\u500b\u4f53\u3092\u9078\u629c\u3059\u308b\u307e\u3067\u3001\u305d\u308c\u307e\u3067\u306b\u7a4d\u307f\u4e0a\u3052\u3066\u304d\u305f\u30c8\u30e9\u30f3\u30b9\u30b8\u30a7\u30cb\u30c3\u30af\u30de\u30a6\u30b9\u4f5c\u88fd\u306e\u30ce\u30a6\u30cf\u30a6\u304c\u5f79\u7acb\u3061\u3001\u30b9\u30e0\u30fc\u30ba\u306b\u8ecc\u9053\u306b\u4e57\u308a\u307e\u3057\u305f\u300d\u3068\u57fa\u76e4\u6280\u8853\u306e\u91cd\u8981\u6027\u3092\u6307\u6458\u3057\u307e\u3059\u3002\u305f\u3060\u3057Cas9 DNA\u30d9\u30af\u30bf\u30fc\u5c0e\u5165\u3060\u3051\u3067\u306f\u30ce\u30c3\u30af\u30a4\u30f3\u500b\u4f53\u304c\u601d\u3046\u3088\u3046\u306b\u5f97\u3089\u308c\u305a\u3001\u540c\u6642\u306bmRNA\u3092\u6253\u3061\u8fbc\u3080\u306a\u3069\u8a66\u884c\u932f\u8aa4\u306e\u672b\u3001\u30ac\u30a4\u30c9RNA\u3068\u3068\u3082\u306bCas9\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6253\u3061\u8fbc\u3080\u73fe\u624b\u6cd5\u306b\u843d\u3061\u7740\u304d\u307e\u3057\u305f\u3002<\/p>\n

\u6e21\u908a\u6c0f\u306f\u52b9\u7387\u7684\u306a\u5b9f\u9a13\u306e\u6d41\u308c\u3092\u6b21\u306e\u3088\u3046\u306b\u8aac\u660e\u3057\u307e\u3059\u3002\u300c1\u907a\u4f1d\u5b50\u306e\u6539\u5909\u306b\u306f\u3001200\u500b\u307b\u3069\u306e\u5375\u306b\u30a4\u30f3\u30b8\u30a7\u30af\u30b7\u30e7\u30f3\u3092\u884c\u3044\u3001\u7fcc\u65e5\u3001\u9806\u8abf\u306b\u767a\u751f\u304c\u9032\u30809\u5272\u307b\u3069\u306e2\u7d30\u80de\u671f\u306e\u5375\u3092\u5b50\u5bae\u3078\u79fb\u690d\u3002\u5de6\u53f3\u306e\u5b50\u5bae\u306b10\u500b\u305a\u3064\u79fb\u690d\u3057\u3001\u8a0810\u5339\u7a0b\u5ea6\u306e\u30de\u30a6\u30b9\u3092\u80b2\u3066\u3001\u751f\u307e\u308c\u305f40\u5339\u307b\u3069\u306e\u4ed4\u306e\u4e2d\u304b\u3089\u76ee\u7684\u306e\u907a\u4f1d\u5b50\u578b\u3092\u6301\u3064\u500b\u4f53\u3092\u63a2\u3057\u3001\u30d8\u30c6\u30ed\u306e\u5834\u5408\u306b\u306f\u4f53\u5916\u53d7\u7cbe\u3067\u30db\u30e2\u7cfb\u7d71\u3092\u6a39\u7acb\u3057\u307e\u3059\u3002\u5171\u540c\u7814\u7a76\u8005\u304b\u3089\u4f9d\u983c\u3092\u53d7\u3051\u3001\u907a\u4f1d\u5b50\u6539\u5909\u500b\u4f53\u306e\u5019\u88dc\u3092\u624b\u5143\u306b\u5c4a\u3051\u308b\u307e\u3067\u3001\u304a\u3088\u305d1\u304b\u6708\u307b\u3069\u3067\u3059\u300d\u3002<\/p>\n

\u300c\u907a\u4f1d\u5b50\u7834\u58ca\u3084\u30aa\u30ea\u30b4\u30cc\u30af\u30ec\u30aa\u30c1\u30c9\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u6210\u529f\u7387\u306f8-9\u5272\u3068\u9ad8\u7387\u3067\u3059\u3002\u305f\u3060\u3057\u9577\u3044\u30ec\u30dd\u30fc\u30bf\u30fc\u907a\u4f1d\u5b50\u306a\u3069\u306e\u30ce\u30c3\u30af\u30a4\u30f3\u306b\u306f\u82e6\u52b4\u3057\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u52b9\u7387\u306e\u5411\u4e0a\u3092\u8ffd\u6c42\u3057\u3001\u4ed6\u88fd\u54c1\u306b\u6bd4\u3079\u6bd2\u6027\u304c\u4f4e\u3044\u305f\u3081\u304b\u30de\u30a6\u30b9\u306e\u7740\u5e8a\u7387\u304c\u9ad8\u3044Invitrogen\u2122 GeneArt\u2122 Platinum Cas9\u00a0 Nuclease\u3092\u9078\u3073\u307e\u3057\u305f\u3002\u73fe\u5728\u3001\u30ce\u30c3\u30af\u30a4\u30f3\u306e\u52b9\u7387\u306f1\u5272\u307b\u3069\u306b\u307e\u3067\u4e0a\u304c\u308a\u3001\u307b\u307c\u78ba\u5b9f\u306b\u7cfb\u7d71\u3092\u6a39\u7acb\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u300d\u3068\u8fd1\u85e4\u6c0f\u3068\u6e21\u908a\u6c0f\u306f\u691c\u8a0e\u7d50\u679c\u3092\u8a71\u3057\u307e\u3059\u3002<\/p>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u6280\u8853\u306f\u57fa\u76e4\u6280\u8853\u306e1\u3064<\/span><\/h2>\n

\u5f93\u6765\u306eES\u7d30\u80de\u3092\u4ecb\u3057\u305f\u30ce\u30c3\u30af\u30a4\u30f3\u6280\u8853\u3067\u306f\u3001\u5de8\u5927\u306a\u30d9\u30af\u30bf\u30fc\u3092\u5b9f\u9a13\u3054\u3068\u306b\u4f5c\u3063\u305f\u308a\u3001\u6a39\u7acb\u3055\u308c\u305f\u7279\u5b9a\u306e\u7d30\u80de\u682a\u3057\u304b\u4f7f\u3048\u306a\u304b\u3063\u305f\u308a\u3068\u5236\u9650\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u300c\u4ee5\u524d\u306f\u5e74\u95932\u7cfb\u7d71\u306e\u6a39\u7acb\u304c\u3084\u3063\u3068\u3067\u3057\u305f\u304c\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u6d3b\u7528\u3059\u308b\u3088\u3046\u306b\u306a\u308a\u3001\u5e74\u959320\u7cfb\u7d71\u3092\u6a39\u7acb\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\u3002\u591a\u91cd\u5909\u7570\u4f53\u3092\u4f5c\u88fd\u3067\u304d\u308b\u70b9\u3082\u826f\u3044\u3067\u3059\u306d\u300d\u3068\u8fd1\u85e4\u6c0f\u3002\u81ea\u8eab\u306e\u7814\u7a76\u306b\u3082\u3001\u7cbe\u5b50\u306e\u53d7\u7cbe\u80fd\u306e\u7372\u5f97\u306b\u5fc5\u8981\u306a\u30aa\u30b9\u5074\u30fb\u30e1\u30b9\u5074\u306e\u56e0\u5b50\u3092\u6f70\u3057\u305f\u7cfb\u7d71\u3092\u8907\u6570\u4f5c\u308a\u3001\u89e3\u6790\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002\u300c\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u7814\u7a76\u3059\u308b\u6642\u4ee3\u306f\u7d42\u308f\u308a\u307e\u3057\u305f\u3002\u3053\u308c\u304b\u3089\u306f\u3001\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u304c1\u3064\u306e\u30d4\u30fc\u30b9\u3068\u306a\u308a\u3001\u5168\u4f53\u306e\u30b9\u30c8\u30fc\u30ea\u30fc\u304c\u3067\u304d\u3042\u304c\u308b\u6642\u4ee3\u3067\u3059\u300d\u3002\u8fd1\u85e4\u6c0f\u304c\u6a39\u7acb\u3057\u305f\u907a\u4f1d\u5b50\u6539\u5909\u30de\u30a6\u30b9\u306f\u3001\u56fd\u5185\u5916\u306e\u6570\u3005\u306e\u30d3\u30c3\u30b0\u30d7\u30ed\u30b8\u30a7\u30af\u30c8\u3092\u652f\u3048\u3001\u4eca\u5f8c\u5927\u304d\u306a\u6210\u679c\u306b\u3064\u306a\u304c\u3063\u3066\u3044\u304f\u3088\u3046\u3067\u3059\u3002<\/p>\n

\u6700\u5927\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u3001\u6700\u5c0f\u9650\u306e\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u5207\u65ad\u3092\u5b9f\u73fe!
\nGeneArt Platinum Cas9 Nuclease\uff08\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u4ed8\u304d\uff09<\/span><\/h2>\n

\"GeneArt<\/a>GeneArt Platinum Cas9 Nuclease\u306f\u3001\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u4ed8\u304d\u306e\u91ce\u751f\u578bCas9\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3042\u308a\u3001CRISPR-Cas9\u30b7\u30b9\u30c6\u30e0\u3067\u4f7f\u7528\u3059\u308b\u30b2\u30ce\u30e0\u7de8\u96c6\u30c4\u30fc\u30eb\u3067\u3059\u3002\u30ac\u30a4\u30c9RNA\uff08gRNA\uff09\u3068\u5b89\u5b9a\u3057\u305f\u30ea\u30dc\u30bf\u30f3\u30d1\u30af\u8cea\uff08RNP\uff09\u8907\u5408\u4f53\u3092\u5f62\u6210\u3055\u305b\u3066\u7d30\u80de\u3078\u5c0e\u5165\u3002DNA\u30d5\u30a9\u30fc\u30de\u30c3\u30c8\u3084mRNA\u30b7\u30b9\u30c6\u30e0\u3088\u308a\u3082\u306f\u308b\u304b\u306b\u9ad8\u3044\u7de8\u96c6\u52b9\u7387\u3092\u5b9f\u73fe\u3057\u3001\u7d30\u80de\u304b\u3089\u6025\u901f\u306b\u9664\u53bb\u3055\u308c\u308b\u305f\u3081\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u5207\u65ad\u3092\u6700\u5c0f\u9650\u306b\u6291\u3048\u307e\u3059\u3002<\/p>\n

GeneArt Platinum Cas9 Nuclease\u306e\u8a73\u7d30\u60c5\u5831\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n

\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n

\"NEXT2017\u5e743\u6708\u53f7\"<\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2017\u5e743\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002
\nNEXT\u306e\u30d0\u30c3\u30af\u30ca\u30f3\u30d0\u30fc\u306f\u3053\u3061\u3089<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

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