{"id":13121,"date":"2022-12-13T09:26:38","date_gmt":"2022-12-13T00:26:38","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=13121"},"modified":"2025-01-21T15:18:37","modified_gmt":"2025-01-21T06:18:37","slug":"western-blot-protein-ts-2","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-2\/","title":{"rendered":"\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c2\u56de\uff1a\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8"},"content":{"rendered":"

\"\"<\/p>\n

\u524d\u56de<\/a>\u306f\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u5411\u3051\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac\u306e\u9078\u3073\u65b9\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u6b21\u306e\u30b9\u30c6\u30c3\u30d7\u3068\u3057\u3066\u3001\u3069\u306e\u304f\u3089\u3044\u62bd\u51fa\u3055\u308c\u305f\u304b\u78ba\u8a8d\u3092\u3059\u308b\u305f\u3081\u306b\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u3082\u5e02\u5834\u306b\u3055\u307e\u3056\u307e\u306a\u3082\u306e\u304c\u3042\u3075\u308c\u3066\u304a\u308a\u3001\u679c\u305f\u3057\u3066\u3069\u308c\u304c\u826f\u3044\u306e\u304b\u8ff7\u3063\u3066\u3057\u307e\u3044\u307e\u3059\u3088\u306d\u3002<\/p>\n

\u305d\u306e\u60a9\u307f\u3001\u3053\u306e\u30d6\u30ed\u30b0\u304c\u89e3\u6c7a\u3057\u307e\u3059\uff01\u7b2c2\u56de\u3067\u306f\u3001\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u306e\u9078\u629e\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\u5404\u62bd\u51fa\u8a66\u85ac\u306b\u9069\u3057\u305f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8<\/h2>\n

\"\"<\/p>\n

\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u306b\u306f\u3055\u307e\u3056\u307e\u306a\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u5168\u3066\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u4f7f\u3048\u308b\u4e07\u80fd\u306a\u30ad\u30c3\u30c8\u306f\u5b58\u5728\u3057\u307e\u305b\u3093\u3002\u3069\u3093\u306a\u30ad\u30c3\u30c8\u306b\u3082\u3001\u6e2c\u5b9a\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u3066\u3057\u307e\u3046\u7269\u8cea\u304c\u5b58\u5728\u3059\u308b\u304b\u3089\u3067\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u7269\u8cea\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u3068\u3001\u5b9f\u969b\u306e\u6fc3\u5ea6\u3088\u308a\u3082\u306f\u308b\u304b\u306b\u4f4e\u304f\u306a\u3063\u305f\u308a\u3001\u9ad8\u304f\u306a\u3063\u305f\u308a\u3057\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u62bd\u51fa\u65b9\u6cd5\u306b\u5408\u308f\u305b\u305f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u3092\u9078\u3076<\/b>\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u524d\u56de\u306f\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u7528\u306e\u62bd\u51fa\u8a66\u85ac\u3068\u3057\u3066SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3057\u305f\u3002\u3082\u3057\u304b\u3059\u308b\u3068\u3001\u7591\u554f\u306b\u601d\u3046\u4eba\u304c\u3044\u308b\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u300cSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6eb6\u304b\u3057\u305f\u3089\u3001\u6fc3\u5ea6\u306e\u6e2c\u5b9a\u304c\u3067\u304d\u306a\u3044\u3093\u3058\u3083\u306a\u3044\u304b\uff1f\u300d<\/p>\n

\u305d\u306e\u3068\u304a\u308a\u3067\u3059\u3002\u5e02\u5834\u306b\u3042\u308b\u307b\u3068\u3093\u3069\u306e\u30ad\u30c3\u30c8\u3067\u306fSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u4e2d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u6e2c\u5b9a\u304c\u3067\u304d\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001SDS<\/b>\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6eb6\u304b\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u3092\u6e2c\u5b9a\u3067\u304d\u308b\u30ad\u30c3\u30c8<\/b>\u306f\u3001\u3061\u3083\u3093\u3068\u5b58\u5728\u3057\u307e\u3059\u3002<\/p>\n

\u307e\u305a\u306f\u524d\u56de\u306e\u30d6\u30ed\u30b0<\/a>\u3067\u3054\u7d39\u4ecb\u3057\u305f\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3059\u3059\u3081\u306e\u62bd\u51fa\u8a66\u85ac\u3054\u3068\u306b\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u8868\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u304a\u3059\u3059\u3081\u306e\u62bd\u51fa\u8a66\u85ac\u306b\u9069\u5408\u3059\u308b\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8<\/p>\n\n\n\n\n\n\n\n
\u62bd\u51fa\u65b9\u6cd5<\/th>\n\u62bd\u51fa\u8a66\u85ac<\/th>\n\u9069\u5408\u3059\u308b\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8<\/th>\n\u30a2\u30c3\u30bb\u30a4\u6642\u306e\u6ce8\u610f\u70b9<\/th>\n<\/tr>\n
\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa
\n(Whole Cell)<\/td>\n		SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc<\/a>
\n\u203bLaemmli\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n		Pierce 660nm Protein Assay Kit<\/a>\u3001
\nIonic Detergent Compatibility Reagent\uff08IDCR\uff09<\/a>\u3092\u7528\u3044\u3066\u300110\u500d\u5e0c\u91c8\u3067\u6e2c\u5b9a<\/td>\n		Bolt<\/a>\uff0fNuPAGE LDS Sample Buffer<\/a>\u3084Invitrogen\u2122 Novex\u2122 Tricine SDS Sample Buffer<\/a>\u306fPierce 660nm Protein Assay Kit\u3067\u6e2c\u5b9a\u3067\u304d\u307e\u305b\u3093\u3002<\/strong><\/td>\n<\/tr>\n
RIPA\u30d0\u30c3\u30d5\u30a1\u30fc<\/a><\/td>\nThermo Scientific\u2122 Pierce\u2122 Rapid Gold BCA Protein Assay Kit<\/a>
\nThermo Scientific\u2122 Pierce\u2122 BCA Protein Assay Kit<\/a>
\nThermo Scientific\u2122 Pierce\u2122 BCA Protein Assay Kit \u2013 RAC<\/a>
\nThermo Scientific\u2122 Micro BCA\u2122 Protein Assay Kit<\/a>
\nPierce 660nm Protein Assay Kit<\/a>
\n\u306a\u3069<\/td>\n		RIPA\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u30e1\u30fc\u30ab\u30fc\u306b\u3088\u3063\u3066\u7d44\u6210\u304c\u7570\u306a\u308b\u305f\u3081\u3001\u30e1\u30fc\u30ab\u30fc\u306b\u3088\u3063\u3066\u306f\u3053\u308c\u3089\u306e\u30ad\u30c3\u30c8\u304c\u9069\u7528\u3067\u304d\u306a\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002
\n\u30ad\u30c3\u30c8\u306b\u3088\u3063\u3066\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u8584\u3081\u3066\u6e2c\u5b9a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/td>\n<\/tr>\n
\u5206\u753b\u62bd\u51fa<\/td>\nNE-PER Nuclear and Cytoplasmic Extraction Reagents<\/a><\/td>\nPierce Rapid Gold BCA Protein Assay Kit<\/a>
\nPierce BCA Protein Assay Kit<\/a>
\nPierce BCA Protein Assay Kit \u2013 RAC<\/a>
\nPierce 660nm Protein Assay Kit<\/a>
\n\u306a\u3069<\/td>\n		\u30ad\u30c3\u30c8\u306b\u3088\u3063\u3066\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u8584\u3081\u3066\u6e2c\u5b9a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/td>\n<\/tr>\n
Mem-PER Plus Membrane Protein Extraction Kit<\/a><\/td>\nPierce Rapid Gold BCA Protein Assay Kit<\/a>
\nPierce BCA Protein Assay Kit<\/a>
\nPierce BCA Protein Assay Kit \u2013 RAC<\/a>
\nMicro BCA Protein Assay Kit<\/a>
\nPierce 660nm Protein Assay Kit<\/a>
\n\u306a\u3069<\/td>\n		\u30ad\u30c3\u30c8\u306b\u3088\u3063\u3066\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u3092\u8584\u3081\u3066\u6e2c\u5b9a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u307e\u305a\u3001SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\uff08Laemmli SDS Sample Buffer\uff09\u306b\u9069\u3057\u3066\u3044\u308b\u306e\u306f\u3001Thermo Scientific\u2122 Pierce\u2122 660nm Protein Assay Kit<\/a>\u3067\u3059\u3002\u539f\u7406\u306f\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002\u3053\u306e\u30ad\u30c3\u30c8\u3068\u3001Thermo Scientific\u2122\u00a0Ionic Detergent Compatibility Reagent\uff08IDCR\uff09<\/a>\u3092\u4f7f\u7528\u3059\u308c\u3070\u3001\u5f93\u6765\u6cd5\u3067\u306f\u6e2c\u5b9a\u304c\u96e3\u3057\u304b\u3063\u305fSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u4e2d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u300110\u500d\u5e0c\u91c8\u3067\u6e2c\u5b9a\u3067\u304d\u307e\u3059\u3002\u53cd\u5fdc\u6642\u9593\u304c5\u5206\u3068\u77ed\u304f\u3001\u64cd\u4f5c\u304c\u7c21\u4fbf\u3067\u3001\u754c\u9762\u6d3b\u6027\u5264\u3084\u9084\u5143\u5264\u3068\u306e\u5171\u5b58\u6027\u304c\u9ad8\u3044\u305f\u3081\u3001SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3068\u3069\u307e\u3089\u305a\u6d3b\u8e8d\u306e\u5834\u304c\u591a\u3044\u30ad\u30c3\u30c8\u3067\u3059\u3002<\/p>\n

\u306a\u304a\u3001\u3053\u3053\u3067\u3054\u7d39\u4ecb\u3057\u3066\u3044\u308bSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001\u6700\u3082\u4e00\u822c\u7684\u306b\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u3001Laemmli\u6cd5\u306b\u3088\u308b\u4e0b\u8a18\u7d44\u6210\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u3059\u3002<\/p>\n

\uff1cLaemmli SDS Sample Buffer\u7d44\u6210\uff1e
\n65 mM Tris-HCl, 10% glycerol, 2% SDS, 0.025% bromophenol blue
\n\u203b\u3053\u308c\u306b\u7d42\u6fc3\u5ea650 mM\u3068\u306a\u308b\u3088\u3046DTT\u3092\u6dfb\u52a0\u3057\u3066\u7528\u3044\u307e\u3059\u3002<\/p>\n

SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u306f\u3055\u307e\u3056\u307e\u306a\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u3067\u8ca9\u58f2\u3057\u3066\u3044\u308bInvitrogen\u2122 Bolt\u2122 LDS Sample Buffer<\/a>\u3084NuPAGE\u2122 LDS Sample Buffer<\/a>\u306f\u3001\u4e2d\u306b\u542b\u307e\u308c\u3066\u3044\u308b\u8272\u7d20\u306f660 nm\u306b\u5438\u53ce\u304c\u3042\u308b\u305f\u3081\u3001Pierce\u2122 660nm Protein Assay<\/u><\/strong>\u304c\u4f7f\u7528\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044<\/strong><\/u>\u3002<\/p>\n

\u6b21\u306b\u3001RIPA\u30d0\u30c3\u30d5\u30a1\u30fc\u3084Thermo Scientific\u2122 NE-PER\u2122 Nuclear and Cytoplasmic Extraction Reagents<\/a>\u3001Mem-PER\u2122 Plus Membrane Protein Extraction Kit<\/a>\u306f\u3001Pierce 660nm Protein Assay Kit<\/a>\u3084BCA\u6cd5\u306e\u8907\u6570\u306e\u30ad\u30c3\u30c8\u3082\u9069\u5408\u3057\u3066\u3044\u307e\u3059\u3002BCA\u6cd5\u306e\u539f\u7406\u3084\u5404\u30ad\u30c3\u30c8\u306e\u7279\u5fb4\u306f\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002BCA\u6cd5\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u3088\u308b\u767a\u8272\u306e\u3070\u3089\u3064\u304d\u304c\u5c11\u306a\u304f\u3001\u754c\u9762\u6d3b\u6027\u5264\u3068\u306e\u5171\u5b58\u6027\u304c\u9ad8\u3044\u70b9\u304c\u7279\u9577\u3067\u3001\u591a\u304f\u306e\u7814\u7a76\u8005\u306b\u975e\u5e38\u306b\u3088\u304f\u4f7f\u7528\u3055\u308c\u3066\u304d\u305f\u6b74\u53f2\u306e\u9577\u3044\u624b\u6cd5\u3067\u3059\u3002<\/p>\n

\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u306e\u63a2\u3057\u65b9<\/h2>\n

\"\"<\/p>\n

\u3067\u306f\u3001\u524d\u7ae0\u3067\u7d39\u4ecb\u3057\u305f\u62bd\u51fa\u8a66\u85ac\u4ee5\u5916<\/strong>\u306e\u65b9\u6cd5\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u62bd\u51fa\u3057\u305f\u5834\u5408\u3001\u3069\u306e\u3088\u3046\u306a\u30ad\u30c3\u30c8\u3067\u6e2c\u5b9a\u3059\u308c\u3070\u3088\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u3084\u307f\u304f\u3082\u306b\u30ad\u30c3\u30c8\u3092\u9078\u3093\u3067\u3057\u307e\u3046\u3068\u3001\u62bd\u51fa\u8a66\u85ac\u306e\u6210\u5206\u304c\u6e2c\u5b9a\u5024\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u3066\u3057\u307e\u3046\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001\u3053\u3061\u3089<\/strong><\/a>\u306e\u8868\u3092\u53c2\u8003\u306b\u3057\u3066\u3001\u62bd\u51fa\u8a66\u85ac\u306b\u9069\u3057\u305f\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u3092\u63a2\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n

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\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u305f\u3081\u306e\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u306e\u9078\u3073\u65b9<\/h2>\n

\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\u306b\u3001\u3069\u3093\u306a\u30b5\u30f3\u30d7\u30eb\u306b\u3082\u9069\u5408\u3067\u304d\u308b\u4e07\u80fd\u306a\u3082\u306e\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u4f8b\u3048\u3070SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3067\u62bd\u51fa\u3057\u305f\u5834\u5408\u306fPierce 660nm Protein Assay Kit\u3092\u7528\u3044\u308b\u3088\u3046\u306b\u3001\u62bd\u51fa\u8a66\u85ac\u306b\u5408\u308f\u305b\u3066\u9078\u629e\u3059\u308b\u3053\u3068\u304c\u5927\u5207\u3067\u3059\u3002
\n\u6b21\u56de<\/a>\u306f\u3001\u6e2c\u5b9a\u3057\u305f\u6fc3\u5ea6\u306b\u5fdc\u3058\u3066\u3001\u3044\u3088\u3044\u3088\u96fb\u6c17\u6cf3\u52d5\u3092\u958b\u59cb\u3057\u307e\u3057\u3087\u3046\u3002\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u6700\u521d\u306e\u5c71\u5834\u3001\u300cSDS-PAGE\u306e\u30b2\u30eb\u3084\u8a66\u85ac\u306e\u9078\u3073\u65b9\u300d\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u30b7\u30ea\u30fc\u30ba\u306f\u3053\u3061\u3089\uff1a
\n\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c1\u56de\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa\u65b9\u6cd5<\/a>
\n\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c2\u56de\uff1a\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8
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\n\u8ee2\u5199\u6761\u4ef6\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c4\u56de<\/a>
\n\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c5\u56de<\/a>
\n\u6297\u4f53\u3092\u9078\u3076\u3068\u304d\u306b\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u3053\u3068\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u7b2c6\u56de<\/a>
\n\u5316\u5b66\u767a\u5149\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306e\u9078\u629e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u3000\u7b2c7\u56de<\/a><\/p>\n

\u3053\u3093\u306a\u30d6\u30ed\u30b0\u3082\u304a\u30b9\u30b9\u30e1\u3067\u3059\u3002
\nBCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de<\/a>
\n\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u307e\u3068\u3081\u7d9a\u304d\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01UV\u6cd5\u3084\u86cd\u5149\u6cd5\u3092\u4f7f\u3046\u306e\u306f\u3069\u3093\u306a\u3068\u304d\uff1f<\/a><\/p>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n

\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

 <\/p>\n

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- Learning at the Bench<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-2\/\" \/>\n<meta property=\"og:locale\" content=\"ja_JP\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c2\u56de\uff1a\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8\" \/>\n<meta property=\"og:description\" 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