{"id":1314,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_39_2_gibcoexpi\/"},"modified":"2023-12-27T17:56:38","modified_gmt":"2023-12-27T08:56:38","slug":"user_voice_39_2_gibcoexpi","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_39_2_gibcoexpi\/","title":{"rendered":"\u5206\u6ccc\u578bIgA\u6297\u4f53\u3092\u8a98\u5c0e\u3059\u308b\u7c98\u819c\u30ef\u30af\u30c1\u30f3\u3084\u65b0\u305f\u306a\u6297\u4f53\u533b\u85ac\u3078\u306e\u5fdc\u7528\u3092\u76ee\u6307\u3057\u3066\uff08\u56fd\u7acb\u611f\u67d3\u75c7\u7814\u7a76\u6240 \u9577\u8c37\u5ddd\u79c0\u6a39 \u6c0f\u3001\u4f50\u91ce\u82b3\u6c0f\uff09-\u300cNEXT\u300d2017\u5e743\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"

\u591a\u7a2e\u985e\u306e\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3092Expi293 Expression System\u3067\u78ba\u5b9f\u306b\u56de\u53ce
\n<\/span><\/h2>\n

\u9577\u8c37\u5ddd\u79c0\u6a39 \u6c0f\uff08\u56fd\u7acb\u611f\u67d3\u75c7\u7814\u7a76\u6240\u611f\u67d3\u75c5\u7406\u90e8\u90e8\u9577\uff09<\/strong>
\n\u4f50\u91ce \u82b3 \u6c0f\uff08\u56fd\u7acb\u611f\u67d3\u75c7\u7814\u7a76\u6240\u7814\u7a76\u751f\u3001\u6771\u5317\u5927\u5b66\u5927\u5b66\u9662\u751f\uff09
\n<\/strong><\/p>\n

\"\u9577\u8c37\u5ddd\u79c0\u6a39<\/a>\u56fd\u7acb\u611f\u67d3\u75c7\u7814\u7a76\u6240\u90e8\u9577\u306e\u9577\u8c37\u5ddd\u79c0\u6a39\u6c0f\u306f\u3001\u30d2\u30c8\u7c98\u819c\u306e\u514d\u75ab\u53cd\u5fdc\u306e\u4e2d\u5fc3\u3068\u306a\u308bIgA\u6297\u4f53\u304c\u3001\u5f93\u6765\u77e5\u3089\u308c\u3066\u3044\u305f2\u91cf\u4f53\u3060\u3051\u3067\u306f\u306a\u304f\u30013\u91cf\u4f53\u30014\u91cf\u4f53\u306a\u3069\u306e\u591a\u91cf\u4f53\u3092\u5f62\u6210\u3059\u308b\u3053\u3068\u3067\u3001\u4e2d\u548c\u6297\u4f53\u4fa1\u3084\u4ea4\u53c9\u9632\u5fa1\u80fd\uff08\u5c11\u3057\u7570\u306a\u308b\u6297\u539f\u306b\u3082\u5bfe\u51e6\u3059\u308b\u80fd\u529b\uff09\u304c\u5411\u4e0a\u3059\u308b\u3053\u3068\u3092\u5831\u544a\u3057\u307e\u3057\u305f\u203b\u3002\u3055\u3089\u306bin vitro<\/i> \u3067\u3082\u78ba\u8a8d\u3057\u3064\u3064\u3001\u3053\u306e\u6210\u679c\u3092\u57fa\u306b\u3001\u3088\u308a\u4e88\u9632\u52b9\u679c\u304c\u9ad8\u304f\u3001\u3088\u308a\u7c21\u4fbf\u306b\u63a5\u7a2e\u3067\u304d\u308b\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30ef\u30af\u30c1\u30f3\u306e\u958b\u767a\u3084\u65b0\u305f\u306a\u6297\u4f53\u533b\u85ac\u54c1\u306e\u958b\u767a\u3092\u76ee\u6307\u3057\u3066\u3044\u307e\u3059\u3002\u9577\u8c37\u5ddd\u6c0f\u306e\u3082\u3068\u3067\u7814\u7a76\u3092\u9032\u3081\u308b\u6771\u5317\u5927\u5b66\u5927\u5b66\u9662\u751f\u306e\u4f50\u91ce\u82b3\u6c0f\u306f\u3001\u6297\u539f\u3084\u6297\u4f53\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u4f5c\u88fd\u306bGibco\u2122 Expi293\u2122 ExpressionSystem\u3092\u4f7f\u3063\u3066\u3044\u307e\u3059\u3002\u300c\u5f93\u6765\u306e\u63a5\u7740\u7d30\u80de\u306e\u7cfb\u306b\u6bd4\u3079\u3066\u3001\u3053\u306e\u30b7\u30b9\u30c6\u30e0\u306f\u6d6e\u904a\u7d30\u80de\u3092\u4f7f\u3046\u306e\u3067\u3001\u6271\u3044\u3084\u3059\u304f\u53ce\u91cf\u3082\u5897\u3048\u307e\u3057\u305f\u300d\u3068\u8a9e\u308a\u307e\u3059\u3002<\/p>\n

\u9ad8\u3044\u53ce\u91cf\u304c\u5f97\u3089\u308c\u308bExpi293 Expression System\u3067\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3082\u30b9\u30e0\u30fc\u30ba\u306b<\/span><\/h2>\n

\u300c\u3053\u308c\u307e\u3067\u306bIgA\u6297\u4f53\u3092\u7d04100\u7a2e\u985e\u3001\u30d8\u30de\u30b0\u30eb\u30c1\u30cb\u30f3\uff08HA\uff09\u6297\u539f\u309210\u6570\u7a2e\u985e\u307b\u3069\u767a\u73fe\u3055\u305b\u3001\u4ea4\u53c9\u6027\u306e\u9ad8\u3044\u6297\u4f53\u3092\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u3057\u3066\u304d\u307e\u3057\u305f\u3002\u3053\u306e\u767a\u73fe\u30ad\u30c3\u30c8\u3092\u4f7f\u3046\u3053\u3068\u3067\u3001\u78ba\u5b9f\u306b\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u56de\u53ce\u3067\u304d\u307e\u3059\u300d\u3068\u4f50\u91ce\u6c0f\u306f\u8a9e\u308a\u307e\u3059\u3002\u300c\u30ad\u30c3\u30c8\u306b\u542b\u307e\u308c\u308bExpi293F\u7d30\u80de\u306f\u3001\u9ad8\u5bc6\u5ea6\u57f9\u990a\u3067\u3082\u584a\u306b\u306a\u3089\u305a\u3001\u304d\u308c\u3044\u306a\u7403\u5f62\u3067\u5897\u3048\u3066\u3044\u304d\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u7d99\u4ee3\u306a\u3069\u306e\u4f5c\u696d\u3082\u697d\u3067\u7d30\u80de\u3082\u6570\u3048\u3084\u3059\u304f\u3001\u5b9f\u9a13\u306b\u5408\u308f\u305b\u3066\u7d30\u80de\u3092\u6e96\u5099\u3067\u304d\u3001\u5b9f\u9a13\u30b9\u30b1\u30b8\u30e5\u30fc\u30eb\u3082\u7acb\u3066\u3084\u3059\u3044\u3067\u3059\u306d\u300d\u3068\u7d9a\u3051\u307e\u3059\u3002\u57f9\u990a\u306f\u3001\u30a4\u30f3\u30ad\u30e5\u30d9\u30fc\u30bf\u30fc\u5185\u306b\u3001\u6e7f\u6c17\u306b\u5f37\u3044\u30b7\u30a7\u30fc\u30ab\u30fc\u3092\u8a2d\u7f6e\u3057\u3066CO2\u6fc3\u5ea68\uff05\u3067\u884c\u3063\u3066\u3044\u307e\u3059\u3002\u300c200mL\u306e\u57f9\u990a\u7cfb\u306a\u3089\u3001HA\u6297\u539f\u3067\u306f4mg\u304b\u30898mg\u306e\u53ce\u91cf\u304c\u5f97\u3089\u308c\u307e\u3059\u3002\u6297\u4f53\u306f\u591a\u3044\u5834\u5408\u306b\u306f30mg\u3050\u3089\u3044\u56de\u53ce\u3067\u304d\u305f\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3088\u300d\u3002IgA\u6297\u4f53\u306e\u7cbe\u88fd\u306fThermo Scientific\u2122 CaptureSelect\u2122 \u3092\u4f7f\u3044\u3001HA\u6297\u539f\u306f\u7d14\u5ea6\u3092\u4e0a\u3052\u308b\u305f\u3081\u306b\u3001His\u30bf\u30b0\u3068Strep\u30bf\u30b0\u306b\u3088\u308b2\u6bb5\u968e\u306e\u30a2\u30d5\u30a3\u30cb\u30c6\u30a3\u7cbe\u88fd\u3092\u884c\u3046\u3068\u304b\u3002\u300c\u30b9\u30af\u30ea\u30fc\u30cb\u30f3\u30b0\u7528\u306b\u767a\u73fe\u3055\u305b\u308b\u5834\u5408\u306b\u306f\u30016\u7a74\u306e\u57f9\u990a\u76bf\u3067\u884c\u3046\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u30022mL\u3068\u3044\u3046\u5c11\u91cf\u306e\u7cfb\u3067\u3082\u3001Expi293Expression System\u306a\u3089\u5341\u5206\u306a\u91cf\u3092\u5f97\u3089\u308c\u307e\u3059\u300d\u3068\u8a71\u3057\u307e\u3059\u3002<\/p>\n

\u5b89\u5168\u3067\u3088\u308a\u52b9\u679c\u306e\u9ad8\u3044\u7d4c\u9f3b\u30ef\u30af\u30c1\u30f3\u3084\u65b0\u3057\u3044\u6297\u4f53\u533b\u85ac\u54c1\u306e\u958b\u767a\u3078<\/span><\/h2>\n

\u300e\u81ea\u7136\u611f\u67d3\u306b\u3088\u3063\u3066\u8a98\u5c0e\u3055\u308c\u305f\u514d\u75ab\u306f\u3001\u30ef\u30af\u30c1\u30f3\u63a5\u7a2e\u306b\u3088\u308b\u514d\u75ab\u3088\u308a\u3082\u3001\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u3078\u306e\u4ea4\u53c9\u9632\u5fa1\u80fd\u304c\u9ad8\u3044\u300f\u3068\u3044\u3046\u4e8b\u5b9f\u306f\u3001\u3059\u3067\u306b1960\u5e74\u4ee3\u304b\u3089\u77e5\u3089\u308c\u3066\u3044\u307e\u3057\u305f\u3002\u9577\u8c37\u5ddd\u6c0f\u306f\u3001\u300c\u305d\u306e\u8981\u56e0\u306e\u4e00\u3064\u306f\u3001\u81ea\u7136\u611f\u67d3\u306b\u3088\u3063\u3066\u8a98\u5c0e\u3055\u308c\u308bIgA\u6297\u4f53\u305d\u306e\u3082\u306e\u3067\u306f\u306a\u3044\u304b\u300d\u3068\u3044\u3046\u30a2\u30a4\u30c7\u30a2\u306e\u3082\u3068\u3001\u9f3b\u306e\u7c98\u819c\u306b\u30ef\u30af\u30c1\u30f3\u3092\u63a5\u7a2e\u3059\u308b\u3001\u7d4c\u9f3b\u30ef\u30af\u30c1\u30f3\u306e\u7814\u7a76\u3092\u7d9a\u3051\u3066\u304d\u307e\u3057\u305f\u3002\u3055\u3089\u306b\u591a\u91cf\u4f53\u5316\u3057\u305fIgA\u6297\u4f53\u304c\u30012\u91cf\u4f53\u3088\u308a\u3082\u9ad8\u3044\u4e2d\u548c\u6d3b\u6027\u3084\u4ea4\u53c9\u80fd\u3092\u793a\u3059\u3053\u3068\u3092\u30d2\u30c8\u306e\u30b5\u30f3\u30d7\u30eb\u3067\u78ba\u8a8d\u203b\u3002\u5f93\u6765\u306e\u76ae\u4e0b\u6ce8\u5c04\u30ef\u30af\u30c1\u30f3\u3067\u306f\u30a6\u30a4\u30eb\u30b9\u611f\u67d3\u5f8c\u306e\u767a\u75c7\u3092\u6291\u3048\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u3059\u304c\u3001\u611f\u67d3\u305d\u306e\u3082\u306e\u3092\u59a8\u3052\u308b\u3053\u3068\u306f\u3067\u304d\u307e\u305b\u3093\u3002\u307e\u305f\u305d\u306e\u52b9\u679c\u306f\u6d41\u884c\u682a\u306b\u9650\u5b9a\u3055\u308c\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u300c\u591a\u91cf\u4f53IgA\u6297\u4f53\u3092\u52b9\u679c\u7684\u306b\u8a98\u5c0e\u3067\u304d\u308b\u3088\u3046\u306a\u30ef\u30af\u30c1\u30f3\u3067\u3042\u308c\u3070\u3001\u9ad8\u3044\u611f\u67d3\u9632\u5fa1\u80fd\u304c\u671f\u5f85\u3067\u304d\u307e\u3059\u300d\u3002\u307e\u305f\u9577\u8c37\u5ddd\u6c0f\u3089\u306f\u8a66\u884c\u932f\u8aa4\u306e\u7d50\u679c\u3001in vitro<\/i> \u3067IgA\u6297\u4f53\u306e\u591a\u91cf\u4f53\u3092\u4f5c\u308b\u3053\u3068\u306b\u3082\u6210\u529f\u3057\u307e\u3057\u305f\u3002\u300cIgA\u6297\u4f53\u4f5c\u88fd\u306b\u306f\u3001H\u9396\u3001L\u9396\u3001J\u9396\u3001SC\uff08\u5206\u6ccc\u30b3\u30f3\u30dd\u30fc\u30cd\u30f3\u30c8\uff09\u306e4\u7a2e\u985e\u3092\u767a\u73fe\u3055\u305b\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u69cb\u7bc9\u3057\u305f\u305d\u308c\u305e\u308c\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u6bd4\u7387\u3092\u5909\u3048\u3066Expi293F \u7d30\u80de\u306b\u540c\u6642\u306b\u611f\u67d3\u3055\u305b\u308b\u3053\u3068\u3067\u3001in vitro<\/i> \u3067\u3082\u591a\u91cf\u4f53\u3092\u5f62\u6210\u3067\u304d\u307e\u3059\u300d\u3002\u7d4c\u9f3b\u30a4\u30f3\u30d5\u30eb\u30a8\u30f3\u30b6\u30ef\u30af\u30c1\u30f3\u306f\u3001\u3059\u3067\u306b\u81e8\u5e8a\u8a66\u9a13\u306e\u6700\u7d42\u6bb5\u968e\u307e\u3067\u9032\u307f\u3001\u6570\u5e74\u5f8c\u306b\u306f\u5b9f\u7528\u5316\u3055\u308c\u308b\u4e88\u5b9a\u3067\u3059\u3002\u300c\u4eca\u5f8c\u306f\u4ed6\u306e\u547c\u5438\u5668\u611f\u67d3\u75c7\u306b\u5bfe\u3059\u308b\u30ef\u30af\u30c1\u30f3\u306e\u958b\u767a\u3082\u624b\u639b\u3051\u305f\u3044\u3002\u307e\u305f\u7c98\u819c\u306f\u547c\u5438\u5668\u3060\u3051\u3067\u306a\u304f\u3001\u8178\u7ba1\u306a\u3069\u4f53\u306e\u4e2d\u3067\u5927\u304d\u306a\u9762\u7a4d\u3092\u5360\u3081\u307e\u3059\u3002\u7c98\u819c\u7279\u6709\u306e\u514d\u75ab\u6a5f\u69cb\u306e\u89e3\u660e\u3084\u3001IgA\u306e\u591a\u91cf\u4f53\u3092\u6297\u4f53\u533b\u85ac\u54c1\u3068\u3057\u3066\u52b9\u679c\u7684\u306b\u5fdc\u7528\u3059\u308b\u7814\u7a76\u7b49\u3082\u88fd\u85ac\u4f01\u696d\u3068\u5354\u529b\u3057\u3064\u3064\u9032\u3081\u305f\u3044\u300d\u3068\u8a71\u3057\u307e\u3059\u3002<\/p>\n

\u203b’Relationship of the quaternary structure of\u00a0human secretory IgA to neutralization of influenza\u00a0virus’ Suzuki T. et al . Proc Natl Acad Sci U S A\u00a0112:7809-7814\uff082015\uff09<\/span><\/p>\n

\u54fa\u4e73\u985e\u7d30\u80de\u3067\u6700\u59271g\/L\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u30b7\u30b9\u30c6\u30e0
\nExpi293 Expression System<\/span><\/h2>\n

\"\"<\/a>\u6d6e\u904a\u7cfb\u306eHEK293\u7d30\u80de\uff08Expi293F\u7d30\u80de\uff09\u3067\u3001\u7d44\u63db\u3048\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5927\u91cf\u306b\u4e00\u904e\u6027\u767a\u73fe\u3055\u305b\u308b\u30c8\u30fc\u30bf\u30eb\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002<\/p>\n

Expi293 Expression System\u306e\u8a73\u7d30\u60c5\u5831\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n

\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n

\"NEXT2017\u5e743\u6708\u53f7\"<\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2017\u5e743\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002
\nNEXT\u306e\u30d0\u30c3\u30af\u30ca\u30f3\u30d0\u30fc\u306f\u3053\u3061\u3089<\/a><\/p>\n

 <\/p>\n

 <\/p>\n

 <\/p>\n

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