{"id":13260,"date":"2023-01-30T14:10:32","date_gmt":"2023-01-30T05:10:32","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=13260"},"modified":"2023-12-28T14:34:02","modified_gmt":"2023-12-28T05:34:02","slug":"blue-native-page-ts-1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/blue-native-page-ts-1\/","title":{"rendered":"Blue Native PAGE\u306f\u306a\u305c\u9752\u3044\u306e\u304b\uff1f"},"content":{"rendered":"

\"\"<\/p>\n

Blue Native PAGE\u3002\u540d\u524d\u3060\u3051\u306a\u3089\u805e\u3044\u305f\u3053\u3068\u304c\u3042\u308b\u3068\u3044\u3046\u4eba\u3082\u591a\u3044\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002Blue Native PAGE\u306f\u300cPAGE\u300d\u3067\u3059\u306e\u3067\u3001\u300c\u30dd\u30ea\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\uff08P<\/u>oly-A<\/u>crylamide-G<\/u>el-E<\/u>lectrophoresis\u300d\u306e\u624b\u6cd5\u306e\u3072\u3068\u3064\u3067\u3059\u3002<\/p>\n

\u3088\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u30dd\u30ea\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u3068\u3044\u3048\u3070SDS-PAGE\u3067\u3059\u306d\u3002\u8a00\u308f\u305a\u3068\u77e5\u308c\u305f\u3001SDS\u3092\u4f7f\u7528\u3057\u305f\u30dd\u30ea\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u96fb\u6c17\u6cf3\u52d5\u3067\u3059\u3002\u3053\u308c\u306eSDS\uff08\u3084\u9084\u5143\u5264\uff09\u3092\u4f7f\u7528\u3057\u306a\u3044\u30d0\u30fc\u30b8\u30e7\u30f3\u304cNative PAGE\u3002\u3044\u305a\u308c\u3082\u3001\u3069\u3046\u3044\u3046\u624b\u6cd5\u306a\u306e\u304b\u540d\u524d\u304b\u3089\u60f3\u50cf\u304c\u3057\u3084\u3059\u3044\u3067\u3059\u3002<\/p>\n

\u3067\u306f\u3001\u300cBlue\u300dNative PAGE\u3068\u306f\u4f55\u3067\u3057\u3087\u3046\uff1f\u306a\u305c\u300c\u9752\u300d\u306a\u306e\u3067\u3057\u3087\u3046\u304b\u3002Native PAGE\u3068\u3069\u3046\u9055\u3046\u306e\u304b\uff1f
\n\u5b9f\u306f\u3001Blue Native PAGE\u306f\u3001Native PAGE\u306e\u5f31\u70b9\u3092\u514b\u670d\u3057\u305f\u96fb\u6c17\u6cf3\u52d5\u624b\u6cd5\u3067\u3059\u3002\u3067\u306f\u3001Blue Native PAGE\u304c\u3069\u306e\u3088\u3046\u306bNative PAGE\u306e\u5f31\u70b9\u3092\u514b\u670d\u3057\u305f\u306e\u304b\u3001\u306a\u305c\u9752\u3044\u306e\u304b\u3001\u305d\u306e\u79d8\u5bc6\u3092\u66b4\u3044\u3066\u3044\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n

Native PAGE\u306e\u9650\u754c<\/h2>\n

\u307e\u305a\u306f\u3001SDS-PAGE\u306e\u539f\u7406\u306b\u3064\u3044\u3066\u3001\u7c21\u5358\u306b\u5fa9\u7fd2\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002
\nSDS-PAGE\u306f\u3001\u30de\u30a4\u30ca\u30b9\u96fb\u8377\u3092\u6301\u3063\u305f\u30c9\u30c7\u30b7\u30eb\u786b\u9178\u30ca\u30c8\u30ea\u30a6\u30e0\uff08SDS\uff09\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5909\u6027\u3055\u305b\u3001\u3055\u3089\u306b\u9084\u5143\u5264\u306b\u3088\u3063\u3066\u9084\u5143\u3055\u305b\u308b\u3053\u3068\u3067\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5747\u4e00\u306e\u30de\u30a4\u30ca\u30b9\u96fb\u8377\u3092\u6301\u3061\u3001\u76f4\u9396\u72b6\u3068\u306a\u308a\u307e\u3059\u3002\u4e00\u65b9\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30b2\u30eb\u306f\u3001\u5c0f\u3055\u306a\u5206\u5b50\u306f\u7db2\u76ee\u69cb\u9020\u3092\u901a\u308a\u629c\u3051\u3084\u3059\u304f\u3001\u5927\u304d\u306a\u5206\u5b50\u306f\u5f15\u3063\u639b\u304b\u308a\u3084\u3059\u3044\u3068\u3044\u3046\u6027\u8cea\u3092\u6301\u3063\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30de\u30a4\u30ca\u30b9\u96fb\u8377\u3092\u6301\u3061\u76f4\u9396\u72b6\u306b\u306a\u3063\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30b2\u30eb\u306e\u7db2\u76ee\u69cb\u9020\u306e\u901a\u308a\u629c\u3051\u3084\u3059\u3055\u3001\u3064\u307e\u308a\u5206\u5b50\u91cf\u306b\u5f93\u3044\u306a\u304c\u3089\u967d\u6975\u5074\u3078\u5f15\u304d\u5bc4\u305b\u3089\u308c\u3001\u5206\u5b50\u91cf\u306b\u4f9d\u5b58\u3057\u305f\u5206\u96e2\u304c\u3067\u304d\u307e\u3059\u3002
\n\"\"<\/p>\n

\u4e00\u65b9\u3001Native PAGE\u3068\u306f\u3001SDS\u3068\u9084\u5143\u5264\u3092\u4f7f\u7528\u3057\u306a\u3044\u30dd\u30ea\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u3067\u3059\u3002\u3064\u307e\u308a\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5747\u4e00\u306b\u30de\u30a4\u30ca\u30b9\u96fb\u8377\u3067\u8986\u3046\u3053\u3068\u306f\u3057\u307e\u305b\u3093\u306e\u3067\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u81ea\u8eab\u304c\u6301\u3063\u3066\u3044\u308b\u96fb\u8377\u304c\u52dd\u8ca0\u3067\u3059\u3002
\n\u3068\u3053\u308d\u3067\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u96fb\u8377\u306f\u3001pH\u306b\u3088\u3063\u3066\u5909\u5316\u3057\u307e\u3059\u3002\u30a2\u30eb\u30ab\u30ea\u6027\u3067\u306f\u30de\u30a4\u30ca\u30b9\u3001\u9178\u6027\u3067\u306f\u30d7\u30e9\u30b9\u3092\u5448\u3057\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u591a\u304f\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304cNative\u306e\u72b6\u614b\u3092\u4fdd\u3064\u306b\u306f\u4e2d\u6027\u4ed8\u8fd1\u306epH\u3067\u3042\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001pH 8.0\u304f\u3089\u3044\u306e\u5f31\u30a2\u30eb\u30ab\u30ea\u6027\u306e\u30b2\u30eb\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u304c\u3001\u3053\u306epH\u3067\u306f\u3001\u4e00\u90e8\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u30d7\u30e9\u30b9\u96fb\u8377\u3092\u5e2f\u3073\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u305d\u306e\u7d50\u679c\u3001\u30b2\u30eb\u306b\u3059\u3089\u5165\u3089\u305a\u3001\u9006\u306b\u9670\u6975\u5074\u306b\u79fb\u52d5\u3057\u3066\u3057\u307e\u3046\u30bf\u30f3\u30d1\u30af\u8cea\u3059\u3089\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u3053\u306e\u3088\u3046\u306b\u3001\u5f93\u6765\u6cd5\u306eNative PAGE\u3067\u306f\u3001\u967d\u6975\u5074\u306b\u79fb\u52d5\u3057\u3084\u3059\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u3001\u79fb\u52d5\u3057\u3065\u3089\u3044\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u3042\u308b\u3001\u3064\u307e\u308a\u300c\u30bf\u30f3\u30d1\u30af\u8cea\u81ea\u8eab\u306e\u96fb\u8377\u306b\u3088\u3063\u3066\u79fb\u52d5\u5ea6\u304c\u5909\u308f\u3063\u3066\u3057\u307e\u3046\u300d\u3068\u3044\u3046\u5927\u304d\u306a\u58c1\u304c\u7acb\u3061\u306f\u3060\u304b\u3063\u3066\u3044\u308b<\/b>\u306e\u3067\u3059\u3002
\n\"\"<\/p>\n

Native PAGE\u306f\u3042\u308a\u306e\u307e\u307e\u306e\u59ff\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u96fb\u6c17\u6cf3\u52d5\u3067\u304d\u307e\u3059\u3002\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u69cb\u9020\u3084\u9ad8\u6b21\u69cb\u9020\u306e\u5927\u304d\u3055\u3054\u3068\u306b\u5206\u96e2\u304c\u3067\u304d\u307e\u3059\u3057\u3001\u6d3b\u6027\u3092\u4fdd\u3063\u305f\u307e\u307e\u306e\u6cf3\u52d5\u3082\u3082\u3061\u308d\u3093\u53ef\u80fd\u3002SDS-PAGE\u306b\u306f\u306a\u3044\u30e1\u30ea\u30c3\u30c8\u304c\u305f\u304f\u3055\u3093\u3042\u308a\u307e\u3059\u3002
\n\u306a\u306e\u306b\u6cf3\u52d5\u304c\u96e3\u3057\u3044\uff01\u305b\u3081\u3066\u3001\u3069\u3046\u306b\u304bNative\u306e\u72b6\u614b\u3092\u4fdd\u3063\u305f\u307e\u307e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30de\u30a4\u30ca\u30b9\u306b\u30c1\u30e3\u30fc\u30b8\u3067\u304d\u308c\u3070\u3044\u3044\u306e\u306b\u3002\u3053\u3093\u306a\u601d\u60f3\u304b\u3089\u751f\u307e\u308c\u305f\u30a2\u30a4\u30c7\u30a2\u304c\u3001<\/b>Blue Native PAGE<\/b>\u3067\u3059\u3002<\/b><\/p>\n

Blue Native PAGE\u3001\u300c\u9752\u300d\u306e\u6b63\u4f53<\/h2>\n

\u305a\u3070\u308a\u3001\u3053\u306e\u300cBlue\u300d\u306e\u6b63\u4f53\u306fCoomassie Brilliant Blue G-250\uff08\u4ee5\u4e0bCBB G-250\uff09\u3067\u3059\u3002\u305d\u3046\u3001SDS-PAGE\u5f8c\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u30d0\u30f3\u30c9\u3092\u67d3\u3081\u308b\u306e\u306b\u3088\u304f\u4f7f\u308f\u308c\u308b\u3001\u304a\u306a\u3058\u307f\u306e\u8a66\u85ac\u3067\u3059\u3002<\/p>\n

\u3053\u306e\u624b\u6cd5\u3092\u78ba\u7acb\u3055\u305b\u305f\u306e\u306f\u3001Sch\u00e4gger \u304a\u3088\u3073von Jagow\u3089\uff3b1-3\uff3d<\/a>
\n\u3067\u3001\u5f7c\u3089\u306fCBB G-250\u304cSDS\u3068\u540c\u3058\u3088\u3046\u306b\u30de\u30a4\u30ca\u30b9\u30c1\u30e3\u30fc\u30b8\u3092\u6301\u3063\u3066\u3044\u308b\u70b9\u306b\u7740\u76ee\u3057\u307e\u3057\u305f\u3002\u4e00\u65b9\u3001SDS\u3068\u7570\u306a\u308a\u3001CBB G-250\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u8868\u9762\u306b\u5f31\u304f\u7d50\u5408\u3059\u308b\u305f\u3081\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5909\u6027\u3055\u305b\u305a\u3001\u9ad8\u6b21\u69cb\u9020\u3092\u58ca\u3055\u306a\u3044\u3053\u3068\u304c\u308f\u304b\u3063\u3066\u3044\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306eNative\u306e\u72b6\u614b\u3092\u4fdd\u3063\u305f\u307e\u307e\u3001\u30de\u30a4\u30ca\u30b9\u306b\u30c1\u30e3\u30fc\u30b8\u3059\u308b\u3053\u3068\u304c\u3067\u304d\u308b\u306e\u3067\u3059\u3002
\n\"\"
\nBlue Native PAGE\u306e\u5229\u70b9\u306f\u3053\u306e\u70b9\u3060\u3051\u306b\u3068\u3069\u307e\u308a\u307e\u305b\u3093\u3002Blue Native PAGE\u3067\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7b49\u96fb\u70b9\u3092\u6c17\u306b\u3059\u308b\u5fc5\u8981\u304c\u306a\u3044\u305f\u3081\u3001\u4e2d\u6027\u306b\u8fd1\u3044pH\u3067\u5b9f\u65bd\u304c\u3067\u304d\u307e\u3059\u3002\u3055\u3089\u306b\u3001n-\u30c9\u30c7\u30b7\u30eb-\u03b2-D-\u30de\u30eb\u30c8\u30b7\u30c9\uff08DDM\uff09\u3084\u30b8\u30ae\u30c8\u30cb\u30f3\u306a\u3069\u306e\u30de\u30a4\u30eb\u30c9\u306a\u754c\u9762\u6d3b\u6027\u5264\u3092\u7528\u3044\u308c\u3070\u3001CBB G-250\u3068\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7d50\u5408\u3092\u963b\u5bb3\u3059\u308b\u3053\u3068\u306a\u304f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6eb6\u89e3\u6027\u3092\u3042\u3052\u308b\u3053\u3068\u3082\u53ef\u80fd\u3067\u3059\u3002
\n\u3053\u306e\u3088\u3046\u306b\u3001Blue Native PAGE\u306f\u3001Native PAGE\u306e\u5229\u70b9\u3092\u7dad\u6301\u3057\u305f\u307e\u307e\u3001\u305d\u306e\u5f31\u70b9\u3092\u514b\u670d\u3057\u305f\u624b\u6cd5\u3068\u8a00\u3048\u307e\u3059\u3002<\/p>\n

Blue Native PAGE\u3092\u5b9f\u65bd\u3059\u308b\u3068\u304d\u306f\u3001\u967d\u6975\u306e\u6cf3\u52d5\u30d0\u30c3\u30d5\u30a1\u30fc\u3084\u3001\u6761\u4ef6\u306b\u3088\u3063\u3066\u306f\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3082CBB G-250\u3092\u6dfb\u52a0\u3057\u307e\u3059\u3002Blue Native PAGE\u306b\u6b20\u70b9\u304c\u3042\u308b\u3068\u3059\u308c\u3070\u3001\u6cf3\u52d5\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u9752\u3044\u305f\u3081\u306b\u3001\u30a2\u30d7\u30e9\u30a4\u6642\u306b\u30a6\u30a7\u30eb\u304c\u898b\u3065\u3089\u3044\u70b9\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u3057\u304b\u3057\u3001\u30b2\u30eb\u30ab\u30bb\u30c3\u30c8\u306e\u30a6\u30a7\u30eb\u90e8\u5206\u306b\u767d\u3044\u30d3\u30cb\u30fc\u30eb\u30c6\u30fc\u30d7\u3092\u8cbc\u308b\u3060\u3051\u3067\u3001\u3053\u306e\u554f\u984c\u306f\u96e3\u306a\u304f\u89e3\u6c7a\u3067\u304d\u307e\u3059\u3002<\/p>\n

\"\u30b2\u30eb\u30ab\u30bb\u30c3\u30c8\u306e\u30a6\u30a7\u30eb\u90e8\u5206\u306b\u767d\u3044\u30d3\u30cb\u30fc\u30eb\u30c6\u30fc\u30d7\u3092\u8cbc\u3063\u305f\u69d8\u5b50\"

\u30b2\u30eb\u30ab\u30bb\u30c3\u30c8\u306e\u30a6\u30a7\u30eb\u90e8\u5206\u306b\u767d\u3044\u30d3\u30cb\u30fc\u30eb\u30c6\u30fc\u30d7\u3092\u8cbc\u3063\u305f\u69d8\u5b50<\/p><\/div>\n

\u521d\u3081\u3066\u306eBlue Native PAGE\u3001\u5fc5\u8981\u306a\u3082\u306e\u306f\uff1f<\/h2>\n

Blue Native PAGE\u3067\u306fCBB G-250\u3092\u542b\u3093\u3060\u3082\u306e\u3092\u4f7f\u7528\u3059\u308b\u305f\u3081\u3001Native PAGE\u3068\u3082\u3001SDS-PAGE\u3068\u3082\u6d88\u8017\u54c1\u304c\u7570\u306a\u308a\u307e\u3059\u3002Sch\u00e4gger \u304a\u3088\u3073von Jagow\u3089\u306e\u6587\u732e\uff3b1-3\uff3d<\/a>\u3092\u53c2\u8003\u306b\u3057\u3066\u3001\u5fc5\u8981\u306a\u3082\u306e\u3092\u305d\u308d\u3048\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u307e\u305f\u306f\u3001\u5143\u306e\u6587\u732e\u3092\u53c2\u8003\u306b\u3057\u3066\u88fd\u54c1\u5316\u3055\u308c\u305f\u30d7\u30ec\u30ad\u30e3\u30b9\u30c8\u30b2\u30eb\u3084\u30ad\u30c3\u30c8\u304c\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u88681\uff09\u3002<\/p>\n

\u88681. Blue Native PAGE\u3067\u4f7f\u7528\u3059\u308b\u6d88\u8017\u54c1\u203b<\/sup><\/p>\n\n\n\n\n\n\n\n\n
\u6d88\u8017\u54c1<\/td>\n\u88fd\u54c1\u540d<\/td>\n\u5099\u8003<\/td>\n<\/tr>\n
\u30b2\u30eb<\/td>\nInvitrogen\u2122 NativePAGE\u2122 Novex\u2122 4-16%<\/a><\/td>\n\u5206\u5b50\u91cf\u306e\u79fb\u52d5\u5ea6\u30c1\u30e3\u30fc\u30c8\u56f3\u3092\u898b\u308b<\/a><\/td>\n<\/tr>\n
Invitrogen\u2122 NativePAGE\u2122 Novex\u2122 3-12%<\/a><\/td>\n<\/tr>\n
\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\nInvitrogen\u2122 NativePAGE\u2122 Sample Prep Kit<\/a><\/td>\nDDM\u3001\u30b8\u30ae\u30c8\u30cb\u30f3\u304c\u4ed8\u5c5e\u3057\u3066\u3044\u307e\u3059<\/td>\n<\/tr>\n
\u6cf3\u52d5\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\nInvitrogen\u2122 NativePAGE\u2122 Running Buffer Kit<\/a><\/td>\n\u967d\u6975\u3001\u9670\u6975\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u30bb\u30c3\u30c8<\/td>\n<\/tr>\n
\u30de\u30fc\u30ab\u30fc<\/td>\nInvitrogen\u2122 NativeMark\u2122 Unstained Protein Standard<\/a><\/td>\n\u5f93\u6765\u6cd5\u306eNative PAGE\u306b\u3082\u4f7f\u7528\u53ef\u80fd<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b2022\u5e74\u73fe\u5728\u3001Blue Native PAGE\u306e\u30d7\u30ec\u30ad\u30e3\u30b9\u30c8\u30b2\u30eb\u3084\u30d0\u30c3\u30d5\u30a1\u30fc\u30ad\u30c3\u30c8\u306f\u3001\u8868\u5185\u30ea\u30f3\u30af\u5148\u306e\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304b\u3089\u306e\u307f\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n

\u88681\u306e\u30b2\u30eb\u306f\u3001\u3044\u305a\u308c\u3082Invitrogen\u2122 XCell SureLock\u2122<\/a>\u306a\u3069\u306e\u5782\u76f4\u578b\u30df\u30cb\u30bf\u30f3\u30d1\u30af\u8cea\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u30b7\u30b9\u30c6\u30e0\u3067\u6cf3\u52d5\u3067\u304d\u307e\u3059\u3002\u305d\u306e\u307b\u304b\u3001\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\uff08Invitrogen\u2122 PowerEase\u2122 Touch 120W Power Supply<\/a>\u306a\u3069\uff09\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u304c\u3001\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\u3092\u7528\u610f\u3059\u308b\u3068\u304d\u306b\u4e8b\u524d\u306b\u78ba\u8a8d\u3057\u3066\u304a\u304d\u305f\u3044\u6ce8\u610f\u70b9\u304c\u3072\u3068\u3064\u3042\u308a\u307e\u3059\u3002<\/p>\n

Blue Native PAGE\u3067\u306f\u3001\u5b9a\u96fb\u5727\u3067\u96fb\u6c17\u6cf3\u52d5\u3092\u884c\u3044\u307e\u3059\u304c\u3001\u5f90\u3005\u306b\u96fb\u6d41\u5024\u304c\u4f4e\u304f\u306a\u308a\u3001\u6cf3\u52d5\u7d42\u76e4\u306b\u306f2 mA\u4ee5\u4e0b\u306b\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u96fb\u6d41\u306e\u4e0b\u9650\u5024\u304c\u8a2d\u5b9a\u3055\u308c\u3066\u3044\u308b\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\u306e\u5834\u5408\u3001\u6cf3\u52d5\u304c\u7d42\u308f\u308b\u524d\u306b\u300c\u96fb\u6d41\u5024\u304c\u4f4e\u3059\u304e\u308b\u300d\u3068\u3044\u3046\u30a8\u30e9\u30fc\u3067\u6b62\u307e\u3063\u3066\u3057\u307e\u3046\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305a\u306f\u88682\u306e\u4e88\u60f3\u96fb\u6d41\u5024\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u88682. Blue Native PAGE\u5b9f\u884c\u4e2d\u306e\u96fb\u5727\u3001\u96fb\u6d41\u5024<\/p>\n\n\n\n\n\n
\u30b2\u30eb\u306e\u7a2e\u985e<\/b><\/td>\n\u96fb\u5727\u5024<\/b><\/td>\n\u6cf3\u52d5\u6642\u9593<\/b><\/td>\n\u4e88\u60f3\u96fb\u6d41\u5024<\/b> (<\/b>\u30b2\u30eb<\/b>1<\/b>\u679a\u6642<\/b>)<\/b><\/td>\n<\/tr>\n
3-12% NativePAGE\u30b2\u30eb<\/td>\n150 V\u5b9a\u96fb\u5727<\/td>\n90\uff5e115\u5206\u9593<\/td>\n\u958b\u59cb\u6642\uff1a12\uff5e16 mA\u3000\u7d42\u4e86\u6642\uff1a2\uff5e4 mA\u4ee5\u4e0b<\/td>\n<\/tr>\n
4-16% NativePAGE\u30b2\u30eb<\/td>\n150 V\u5b9a\u96fb\u5727<\/td>\n105\uff5e120\u5206\u9593<\/td>\n\u958b\u59cb\u6642\uff1a12\uff5e16 mA\u3000\u7d42\u4e86\u6642\uff1a2\uff5e4 mA\u4ee5\u4e0b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

Blue Native PAGE\u3092\u5b9f\u65bd\u3059\u308b\u524d\u306b\u3001\u3054\u4f7f\u7528\u4e88\u5b9a\u306e\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\u3067\u96fb\u6d41\u5024\u306e\u4e0b\u9650\u5024\u304c\u8a2d\u5b9a\u3055\u308c\u3066\u3044\u306a\u3044\u304b\u3001\u307e\u305f\u3001\u3082\u3057\u8a2d\u5b9a\u3055\u308c\u3066\u3044\u308b\u5834\u5408\u306f\u4e0b\u9650\u5024\u306e\u8a2d\u5b9a\u3092\u89e3\u9664\u3067\u304d\u308b\u304b\u3001\u5fc5\u305a\u78ba\u8a8d\u3092\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n

Blue Native PAGE\u5fdc\u7528\u7de8\u3000\uff5e\u4e8c\u6b21\u5143\u5c55\u958b\u3068\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0<\/h2>\n

Blue Native PAGE\u3067\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8907\u5408\u4f53\u3084\u9ad8\u6b21\u69cb\u9020\u3092\u4fdd\u3063\u305f\u307e\u307e\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u7b49\u96fb\u70b9\u3092\u6c17\u306b\u305b\u305a\u6cf3\u52d5\u3067\u304d\u308b\u512a\u308c\u305f\u624b\u6cd5\u3067\u3059\u3002\u305f\u3068\u3048\u3070\u3001Blue Native PAGE\u3067\u89e3\u6790\u3057\u305f\u8907\u5408\u4f53\u3092\u69cb\u6210\u3057\u3066\u3044\u308b\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf\u304c\u3069\u306e\u304f\u3089\u3044\u304b\u3001\u3055\u3089\u306a\u308b\u89e3\u6790\u3092\u3057\u305f\u3044\u4eba\u3082\u3044\u308b\u304b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n

\u3053\u306e\u3088\u3046\u306a\u3068\u304d\u306b\u304a\u52e7\u3081\u3057\u305f\u3044\u306e\u304c\u3001Blue Native PAGE\u3092\u7528\u3044\u305f\u4e8c\u6b21\u5143\u96fb\u6c17\u6cf3\u52d5\u3067\u3059\u3002\u3064\u307e\u308a\u3001\u4e00\u6b21\u5143\u76ee\u306bBlue Native PAGE\u3092\u5b9f\u65bd\u3057\u3001\u4e8c\u6b21\u5143\u76ee\u306bSDS-PAGE\u3092\u884c\u3044\u307e\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001\u8907\u5408\u4f53\u30fb\u9ad8\u6b21\u69cb\u9020\u3092\u4fdd\u3063\u305f\u307e\u307e\u306e\u5206\u5b50\u91cf\u3068\u3001\u9084\u5143\u3055\u305b\u3066\u9396\u72b6\u306b\u3057\u305f\u5206\u5b50\u91cf\u306e\u4e21\u65b9\u304c\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002
\n\"\"
\n\u4e8c\u6b21\u5143\u5c55\u958b\u3092\u3057\u3066\u691c\u51fa\u3057\u305f\u30b9\u30dd\u30c3\u30c8\u306f\u3001\u8cea\u91cf\u5206\u6790\u3067\u89e3\u6790\u3082\u53ef\u80fd\u3067\u3059\uff3b4\uff3d<\/a>\u3002<\/p>\n

\u307e\u305f\u3001Blue Native PAGE\u3092\u5b9f\u65bd\u3057\u305f\u5f8c\u3001\u7279\u5b9a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3060\u3051\u3092\u691c\u51fa\u3057\u305f\u3044\u7814\u7a76\u8005\u3055\u3093\u3082\u3044\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u306e\u691c\u51fa\u3067\u3059\u3002Blue Native PAGE\u5f8c\u306b\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3092\u884c\u3046\u3053\u3068\u3082\u3001\u3082\u3061\u308d\u3093\u53ef\u80fd\u3067\u3059\u3002
\n\"\"
\nBlue Native PAGE\u5f8c\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u6c17\u3092\u3064\u3051\u308b\u306e\u306f\u3001\u8ee2\u5199\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002\u30e1\u30f3\u30d6\u30ec\u30f3\u306f\u5fc5\u305aPVDF\u3092\u4f7f\u7528\u3057\u3001\u8ee2\u5199\u524d\u306b\u30b2\u30eb\u3092SDS-PAGE\u7528\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a1\u30fc\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6d78\u3059\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u8ee2\u5199\u5f8c\u306f\u30e1\u30f3\u30d6\u30ec\u30f3\u4e0a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u9162\u9178\u3067\u56fa\u5b9a\u3057\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u3067CBB G-250\u3092\u8131\u8272\u3059\u308b\u30b9\u30c6\u30c3\u30d7\u304c\u5165\u308a\u307e\u3059\u3002\u8ffd\u52a0\u306e\u64cd\u4f5c\u306f\u3053\u308c\u304f\u3089\u3044\u3067\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u4ee5\u964d\u306f\u4e00\u822c\u7684\u306a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u6cbf\u3063\u3066\u5b9f\u65bd\u3067\u304d\u307e\u3059\u3002\u8ee2\u5199\u3068\u8ee2\u5199\u5f8c\u306e\u51e6\u7406\u3084\u4e8c\u6b21\u5143\u5c55\u958b\u306b\u3064\u3044\u3066\u306f\u3001Blue Native PAGE\u30e6\u30fc\u30b6\u30fc\u30ac\u30a4\u30c9<\/a>\u3092\u53c2\u8003\u306b\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n

Blue Native PAGE\u304c\u9752\u3044\u306e\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092Native\u306e\u307e\u307e\u30de\u30a4\u30ca\u30b9\u306b\u30c1\u30e3\u30fc\u30b8\u3059\u308b\u305f\u3081<\/h2>\n

Blue Native PAGE\u304c\u9752\u3044\u306e\u306f\u3001CBB G-250\u3092\u7528\u3044\u3066\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u8907\u5408\u4f53\u3084\u9ad8\u6b21\u69cb\u9020\u3092\u7dad\u6301\u3057\u305f\u307e\u307e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30de\u30a4\u30ca\u30b9\u306b\u30c1\u30e3\u30fc\u30b8\u3055\u305b\u308b\u305f\u3081\u3067\u3059\u3002\u3053\u308c\u306b\u3088\u308a\u3001Native PAGE\u306e\u5229\u70b9\u3092\u751f\u304b\u3057\u305f\u307e\u307e\u5f31\u70b9\u3092\u514b\u670d\u3059\u308b\u3053\u3068\u306b\u6210\u529f\u3057\u3001\u5f93\u6765\u6cd5\u3067\u306f\u96e3\u3057\u304b\u3063\u305f\u3055\u307e\u3056\u307e\u306a\u89e3\u6790\u304c\u3067\u304d\u308b\u3088\u3046\u306b\u306a\u308a\u307e\u3057\u305f\uff3b5-8\uff3d<\/a>\u3002\u4e8c\u6b21\u5143\u76ee\u3078\u306e\u5c55\u958b\u3084\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3082\u53ef\u80fd\u3067\u3059\u3002\u5f93\u6765\u6cd5\u306eNative PAGE\u304c\u3046\u307e\u304f\u3044\u304b\u305a\u3001\u3084\u304d\u3082\u304d\u3057\u3066\u3044\u305f\u4eba\u306f\u3001\u305c\u3072Blue Native PAGE\u3092\u8a66\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u3053\u308c\u304b\u3089Blue Native PAGE\u3092\u59cb\u3081\u308b\u65b9\u306b\u3001\u5b9f\u9a13\u306e\u30b3\u30c4\u3068\u30dd\u30a4\u30f3\u30c8\u3092\u3054\u7d39\u4ecb\uff01
\n\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30cf\u30f3\u30ba\u30aa\u30f3\u30c8\u30ec\u30fc\u30cb\u30f3\u30b0III\u3000\uff5eBlue native PAGE\uff5e<\/p>\n

\u8a73\u7d30\u3092\u898b\u308b<\/a><\/p>\n

\u3053\u306e\u30d6\u30ed\u30b0\u3092\u898b\u305f\u4eba\u306f\u3001\u3053\u3093\u306a\u30d6\u30ed\u30b0\u3082\u898b\u3066\u3044\u307e\u3059\u3002
\nBCA\u6cd5\u3001Bradford\u6cd5\u3001Lowry\u6cd5\u306a\u3069\u3001\u201c\u7dcf\u201d\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u6cd5\u306e\u539f\u7406\u307e\u3068\u3081\uff5c\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\uff01\u30bf\u30f3\u30d1\u30af\u8cea\u5b9f\u9a13\u3042\u308c\u3053\u308c \u7b2c4\u56de<\/a>
\n\u3088\u308a\u77ed\u6642\u9593\u3067\u3001\u3088\u308a\u7c21\u5358\u306b\u3002\u9ad8\u901f\u30bf\u30f3\u30d1\u30af\u8cea\u8ee2\u5199\u88c5\u7f6e\u304c\u751f\u307e\u308c\u305f\u30ef\u30b1<\/a><\/p>\n

\u6587\u732e<\/h2>\n

1. Sch\u00e4gger, H., and von Jagow, G. (1991) Blue Native Electrophoresis for Isolation of Membrane Protein Complexes in Enzymatically Active Form. Anal. Biochem. 199:223-231.
\n2. Sch\u00e4gger, H., Cramer, W. A., and von Jagow, G. (1994) Analysis of Molecular Masses and Oligomeric States of Protein Complexes by Blue Native Electrophoresis and Isolation of Membrane Protein Complexes by Two-dimensional Native Electrophoresis. Anal. Biochem. 217:220-230.
\n3. Sch\u00e4gger, H. (2001) Blue Native Gels to Isolate Protein Complexes from Mitochondria. Meth. Cell Biol. 65:231-244.
\n4. Fuqiang Wang, Ling Wang, Zhiyang Xu, Gaolin Liang (2013) Identification and Analysis of Multi-Protein Complexes in Placenta
\n5. Svetlana Konovalova (2019) Analysis of Mitochondrial Respiratory Chain Complexes in Cultured Human Cells using Blue Native Polyacrylamide Gel Electrophoresis and Immunoblotting
\n6. Jichun Ma 1, Di Xia (2008) The use of blue native PAGE in the evaluation of membrane protein aggregation states for crystallization
\n7. Leslie G Nucifora et al. (2012) Identification of novel potentially toxic oligomers formed in vitro from mammalian-derived expanded huntingtin exon-1 protein
\n8. Sebastian Kehrloesser et al. (2016) Intrinsic aggregation propensity of the p63 and p73 TI domains correlates with p53R175H interaction and suggests further significance of aggregation events in the p53 family<\/p>\n

 <\/p>\n

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