{"id":13289,"date":"2023-02-06T10:21:17","date_gmt":"2023-02-06T01:21:17","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=13289"},"modified":"2025-01-21T15:17:29","modified_gmt":"2025-01-21T06:17:29","slug":"western-blot-protein-ts-3","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-3\/","title":{"rendered":"SDS-PAGE\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c3\u56de"},"content":{"rendered":"

\"\"
\n\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u5411\u3051\u306e\u88fd\u54c1\u9078\u3073\u306e\u30dd\u30a4\u30f3\u30c8\u7d39\u4ecb\u30b7\u30ea\u30fc\u30ba\u3001\u7b2c3\u56de\u306fSDS-PAGE\u3067\u3059\u3002<\/p>\n

SDS-PAGE\u306f\u30dd\u30ea\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306e\u7565\u3067\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u691c\u51fa\u3059\u308b\u524d\u306b\u3001\u5206\u5b50\u91cf\u306b\u5fdc\u3058\u3066\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u5206\u96e2\u3059\u308b\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002\u3054\u5b9f\u65bd\u3055\u308c\u305f\u7d4c\u9a13\u304c\u3042\u308b\u65b9\u3082\u591a\u3044\u3067\u3057\u3087\u3046\u3002\u3088\u304f\u5b9f\u65bd\u3055\u308c\u3066\u3044\u308b\u65b9\u6cd5\u3060\u304b\u3089\u3053\u305d\u3001\u591a\u7a2e\u591a\u69d8\u306a\u88fd\u54c1\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3067\u306f\u3001\u304a\u624b\u5143\u306e\u30b5\u30f3\u30d7\u30eb\u306b\u6700\u9069\u306a\u30b2\u30eb\u306f\u3069\u308c\u3067\u3057\u3087\u3046\u304b\u3002\u30de\u30fc\u30ab\u30fc\u306f\u3069\u308c\u3092\u4f7f\u3048\u3070\u3088\u3044\u306e\u304b\uff1f\u30a2\u30d7\u30e9\u30a4\u91cf\u306f\u3069\u306e\u304f\u3089\u3044\uff1f\u60a9\u307f\u306f\u5c3d\u304d\u307e\u305b\u3093\u3002<\/p>\n

\u305d\u306e\u60a9\u307f\u3001\u3053\u306e\u30d6\u30ed\u30b0\u304c\u89e3\u6c7a\u3057\u307e\u3059\uff01\u307e\u305a\u306f\u30b2\u30eb\u306e\u9078\u3073\u65b9\u304b\u3089\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n

SDS-PAGE\u30b2\u30eb\u306e\u9078\u3073\u65b9<\/h2>\n

\"\"
\n1970\u5e74\u4ee3\u306bLaemmli\u3089\u304cSDS-PAGE\u306e\u624b\u6cd5\u3092\u78ba\u7acb\u3057\u3066\u304b\u3089\u3057\u3070\u3089\u304f\u305f\u3061\u307e\u3059\u304c\u3001\u73fe\u4ee3\u306f\u76ee\u7684\u3084\u5206\u5b50\u91cf\u306b\u5fdc\u3058\u3066\u3055\u307e\u3056\u307e\u306a\u30d0\u30c3\u30d5\u30a1\u30fc\u7cfb\u306e\u30b2\u30eb\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30d0\u30c3\u30d5\u30a1\u30fc\u7cfb\u304c\u7570\u306a\u308b\u3068\u3001\u540c\u3058\u30b2\u30eb\u6fc3\u5ea6\u3067\u3082\u3001SDS-PAGE\u3092\u5b9f\u65bd\u3057\u305f\u3068\u304d\u306e\u30d1\u30bf\u30fc\u30f3\u304c\u7570\u306a\u308a\u307e\u3059\u306e\u3067\u3001\u307e\u305a\u306f\u3069\u3046\u3044\u3063\u305f\u30d0\u30c3\u30d5\u30a1\u30fc\u7cfb\u306e\u30b2\u30eb\u3092\u4f7f\u3046\u306e\u304b\u3092\u6c7a\u3081\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u3055\u3066\u3001\u30b2\u30eb\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u7cfb\u306e\u306a\u304b\u3067\u306f\u3001Laemmli\u304c\u958b\u767a\u3057\u305fTris-Glycine\u7cfb\u306e\u5b9f\u7e3e\u304c\u5727\u5012\u7684\u306b\u591a\u3044\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u8ad6\u6587\u3067\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u304b\u3089\u3068\u3044\u3046\u7406\u7531\u3067Tris-Glycine\u7cfb\u306b\u3059\u3050\u7d5e\u3063\u3066\u3057\u307e\u3046\u306e\u306f\u3001\u5c11\u3057\u3082\u3063\u305f\u3044\u306a\u3044\u3067\u3059\u3002\u73fe\u5728\u306f\u3001Tris-Glycine\u7cfb\u306e\u30c7\u30e1\u30ea\u30c3\u30c8\u3092\u30ab\u30d0\u30fc\u3057\u305f\u3001Bis-Tris\u7cfb\u306e\u30b2\u30eb\u3068\u3044\u3046\u3082\u306e\u304c\u958b\u767a\u3055\u308c\u3066\u3044\u308b\u304b\u3089\u3067\u3059\u3002<\/p>\n

\u88681\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u7528\u30fbSDS-PAGE\u30b2\u30eb\u3068\u6cf3\u52d5\u30d0\u30c3\u30d5\u30a1\u30fc \u4e00\u89a7<\/p>\n\n\n\n\n\n\n\n\n
\u5185\u5bb9<\/td>\n\u7279\u5fb4<\/td>\n\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf<\/td>\n\u6cf3\u52d5\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n\u5206\u5b50\u91cf\u3054\u3068\u306e\u79fb\u52d5\u5ea6\u30c1\u30e3\u30fc\u30c8<\/td>\n<\/tr>\n
NuPAGE Bis-Tris\u30b2\u30eb<\/a><\/td>\n\u4e2d\u6027\u30b2\u30eb\u306e\u305f\u3081\u3001\u30b9\u30de\u30a4\u30ea\u30f3\u30b0\u304c\u8d77\u304d\u3065\u3089\u3044\u3002\u5e38\u6e29\u4fdd\u5b58\u3067\u6709\u52b9\u671f\u9650\u304c\u9577\u3044<\/td>\n\u5e45\u5e83\u304f\u30ab\u30d0\u30fc<\/td>\nMES\u30d0\u30c3\u30d5\u30a1\u30fc<\/a>
\n\u307e\u305f\u306f
\nMOPS\u30d0\u30c3\u30d5\u30a1\u30fc<\/a><\/td>\n		\"\"<\/a><\/td>\n<\/tr>\n
Bolt Bis-Tris\u30b2\u30eb<\/a><\/td>\n\u4e2d\u6027\u30b2\u30eb\u306e\u305f\u3081\u3001\u30b9\u30de\u30a4\u30ea\u30f3\u30b0\u304c\u8d77\u304d\u3065\u3089\u3044\u3002\u5e38\u6e29\u4fdd\u5b58\u3067\u6709\u52b9\u671f\u9650\u304c\u9577\u3044\u3002\u304f\u3055\u3073\u578b\u30a6\u30a7\u30eb\u3067\u30b5\u30f3\u30d7\u30eb\u304c\u591a\u304f\u30a2\u30d7\u30e9\u30a4\u3067\u304d\u308b<\/td>\n\u5e45\u5e83\u304f\u30ab\u30d0\u30fc<\/td>\nMES\u30d0\u30c3\u30d5\u30a1\u30fc<\/a>
\n\u307e\u305f\u306f
\nMOPS\u30d0\u30c3\u30d5\u30a1\u30fc<\/a><\/td>\n		\"\"<\/a><\/td>\n<\/tr>\n
Novex Wedgewell Tris-Glycine\u30b2\u30eb<\/a><\/td>\n\u53e4\u304f\u304b\u3089\u4f7f\u308f\u308c\u3066\u304d\u305f\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306a\u30b2\u30eb\u7d44\u6210\u3002\u30a6\u30a7\u30eb\u306f\u72ec\u81ea\u306e\u304f\u3055\u3073\u578b\u30a6\u30a7\u30eb\u3067\u3001\u30b5\u30f3\u30d7\u30eb\u304c\u591a\u304f\u30a2\u30d7\u30e9\u30a4\u3067\u304d\u308b<\/td>\n\u5e45\u5e83\u304f\u30ab\u30d0\u30fc<\/td>\nTris-Glycine\u30d0\u30c3\u30d5\u30a1\u30fc<\/a><\/td>\n\"\"<\/a><\/td>\n<\/tr>\n
NuPAGE Tris-Acetate\u30b2\u30eb<\/a><\/td>\n\u9ad8\u5206\u5b50\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u96e2\u306b<\/td>\n\u9ad8\u5206\u5b50<\/td>\nTris-Acetate\u30d0\u30c3\u30d5\u30a1\u30fc<\/a><\/td>\n\"\"<\/a><\/td>\n<\/tr>\n
Novex Tris-Tricine\u30b2\u30eb<\/a>\u203b<\/sup><\/td>\n\u4f4e\u5206\u5b50\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u30da\u30d7\u30c1\u30c9\u306e\u5206\u96e2\u306b<\/td>\n\u4f4e\u5206\u5b50<\/td>\nTricine\u30d0\u30c3\u30d5\u30a1\u30fc<\/a><\/td>\n\"\"<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u30b2\u30eb\u9078\u3073\u306e3\u3064\u306e\u30dd\u30a4\u30f3\u30c8\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\u30fb\u30b2\u30eb\u9078\u629e\u306e\u30dd\u30a4\u30f3\u30c8<\/b>\u2460<\/b>\u3000\u4e2d\u6027\u306e<\/b>Bis-Tris<\/b>\u30b2\u30eb\u306f\u5b89\u5b9a\u3057\u305f\u30c7\u30fc\u30bf\u304c\u51fa\u3084\u3059\u3044<\/b><\/p>\n

\u958b\u767a\u304b\u3089\u6570\u5341\u5e74\u305f\u3063\u305f\u4eca\u3082\u3001Tris-Glycine\u30b2\u30eb\u306f\u591a\u304f\u306e\u7814\u7a76\u8005\u306b\u611b\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3053\u306e\u30b2\u30eb\u306f\u30a2\u30eb\u30ab\u30ea\u6027\u306e\u305f\u3081\u3001\u5b9f\u306f\u6b8b\u5b58\u3059\u308b\u975e\u91cd\u5408\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u304c\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u30b7\u30b9\u30c6\u30a4\u30f3\u6b8b\u57fa\u304a\u3088\u3073\u30ea\u30b8\u30f3\u6b8b\u57fa\u3068\u53cd\u5fdc\u3059\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002
\nBis-Tris\u30b2\u30eb\u306f\u3053\u306e\u5f31\u70b9\u3092\u30ab\u30d0\u30fc\u3059\u308b\u305f\u3081\u306b\u958b\u767a\u3055\u308c\u305f\u4e2d\u6027\u306e\u30b2\u30eb\u3067\u3001\u3053\u306e\u3088\u3046\u306a\u53cd\u5fdc\u304c\u8d77\u3053\u308a\u3065\u3089\u304f\u3001\u3088\u308a\u30b7\u30e3\u30fc\u30d7\u306a\u30d0\u30f3\u30c9\u304c\u5f97\u3089\u308c\u3084\u3059\u3044\u3067\u3059\u3002\u307e\u305f\u3001MES\u3068MOPS\u306e\u6cf3\u52d5\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u3088\u3063\u3066\u79fb\u52d5\u5ea6\u304c\u7570\u306a\u308b\u305f\u3081\u3001\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf\u306b\u5fdc\u3058\u3066\u4f7f\u3044\u5206\u3051\u308b\u3053\u3068\u3082\u53ef\u80fd\u3002\u3055\u3089\u306b\u3001Bis-Tris\u30b2\u30eb\u306f\u5e38\u6e29\u4fdd\u5b58\u3067Tris-Glycine\u3088\u308a\u6709\u52b9\u671f\u9650\u3082\u9577\u3081\u306e\u305f\u3081\u3001\u307e\u3060\u4f7f\u7528\u3057\u305f\u3053\u3068\u304c\u306a\u3044\u4eba\u306f\u3001\u305c\u3072\u8a66\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u30fb\u30b2\u30eb\u9078\u629e\u306e\u30dd\u30a4\u30f3\u30c8<\/b>\u2461<\/b>\u3000\u79fb\u52d5\u5ea6\u30c1\u30e3\u30fc\u30c8\u3092\u898b\u3066\u3001\u76ee\u7684\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u96e2\u304c\u6700\u3082\u826f\u3044\u30b2\u30eb\u3092\u9078\u307c\u3046<\/b><\/p>\n

SDS-PAGE\u3067\u306f\u3001\u30b2\u30eb\u6fc3\u5ea6\u306b\u3088\u3063\u3066\u79fb\u52d5\u5ea6\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u88681\u306e\u300c\u79fb\u52d5\u5ea6\u30c1\u30e3\u30fc\u30c8\u300d\u3092\u53c2\u8003\u306b\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf\u4ed8\u8fd1\u306e\u5206\u96e2\u80fd\u304c\u9ad8\u304f\u3001\u304b\u3064\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u4e2d\u592e\u4ed8\u8fd1\u306b\u6765\u308b\u3088\u3046\u306a\u30b2\u30eb\u3092\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u306a\u304a\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf\u304c\u4e0d\u660e\u306a\u5834\u5408\u306f\u3001\u3088\u308a\u5e83\u304f\u5206\u5b50\u91cf\u3092\u30ab\u30d0\u30fc\u3067\u304d\u308b\u30b0\u30e9\u30b8\u30a8\u30f3\u30c8\u30b2\u30eb\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002<\/p>\n

\u30fb\u30b2\u30eb\u9078\u629e\u306e\u30dd\u30a4\u30f3\u30c8<\/b>\u2462<\/b>\u3000\u30a6\u30a7\u30eb\u6570\u3068\u30b2\u30eb\u539a\u306f\u3001\u30a2\u30d7\u30e9\u30a4\u91cf\u3082\u8003\u3048\u3066\u9078\u629e<\/b><\/p>\n

\u30a6\u30a7\u30eb\u6570\u306f\u5206\u6790\u3057\u305f\u3044\u30b5\u30f3\u30d7\u30eb\u6570\u306b\u5fdc\u3058\u3066\u6c7a\u3081\u307e\u3059\u304c\u3001\u30b5\u30f3\u30d7\u30eb\u6570\u3060\u3051\u3067\u6c7a\u3081\u3066\u3057\u307e\u3046\u3068\u56f0\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u308c\u306f\u3001\u300c\u30a6\u30a7\u30eb\u306b\u30b5\u30f3\u30d7\u30eb\u304c\u5165\u308a\u304d\u3089\u306a\u3044\u5834\u5408\u300d\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6fc3\u5ea6\u304c\u4f4e\u3044\u3068\u3001\u30a2\u30d7\u30e9\u30a4\u3059\u308b\u5bb9\u91cf\u3092\u5897\u3084\u3055\u306a\u3051\u308c\u3070\u306a\u308a\u307e\u305b\u3093\u304c\u3001\u30a6\u30a7\u30eb\u6570\u306b\u3088\u3063\u3066\u306f\u5165\u308a\u304d\u3089\u306a\u3044\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u30a6\u30a7\u30eb\u6570\u304c\u591a\u3044\u307b\u3069\u3001\u30a2\u30d7\u30e9\u30a4\u3067\u304d\u308b\u5bb9\u91cf\u3082\u5c0f\u3055\u304f\u306a\u308a\u307e\u3059\u306e\u3067\u3001\u30a6\u30a7\u30eb\u6570\u306f\u3001\u6b21\u7ae0\u306e\u300c\u30b5\u30f3\u30d7\u30eb\u30a2\u30d7\u30e9\u30a4\u91cf\u306e\u6c7a\u3081\u65b9\u300d\u3082\u53c2\u8003\u306b\u3057\u3066\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u30b5\u30f3\u30d7\u30eb\u30a2\u30d7\u30e9\u30a4\u91cf\u306e\u6c7a\u3081\u65b9<\/h2>\n

\"\"<\/p>\n

\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u30b5\u30f3\u30d7\u30eb\u30a2\u30d7\u30e9\u30a4\u91cf\u306e\u76ee\u5b89\u306f\u3001\u4ee5\u4e0b\u306e\u3068\u304a\u308a\u3067\u3059\u3002<\/p>\n

\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3051\u308b<\/b>SDS-PAGE<\/b>\u30b5\u30f3\u30d7\u30eb\u30a2\u30d7\u30e9\u30a4\u91cf\u306e\u76ee\u5b89\uff1a<\/b>
\n\u7c97\u30b5\u30f3\u30d7\u30eb\uff1a<\/b>20 \u03bcg<\/b>\u7a0b\u5ea6\u3001\u7cbe\u88fd\u30bf\u30f3\u30d1\u30af\u8cea\uff1a<\/b>100 ng\u7a0b\u5ea6<\/b><\/b><\/p>\n

\u3053\u308c\u3089\u306e\u5024\u3068\u3001\u7b2c2\u56de<\/a>\u3067\u6e2c\u5b9a\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u6fc3\u5ea6\u304b\u3089\u3001\u30b5\u30f3\u30d7\u30eb\u306e\u30a2\u30d7\u30e9\u30a4\u306b\u5fc5\u8981\u306a\u5bb9\u91cf\u3092\u6c7a\u5b9a\u3057\u307e\u3057\u3087\u3046\u3002\u3082\u3061\u308d\u3093\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u30d0\u30f3\u30c9\u691c\u51fa\u306b\u306f\u3055\u307e\u3056\u307e\u306a\u8981\u7d20\u304c\u7d61\u307f\u307e\u3059\u306e\u3067\u3001\u6761\u4ef6\u691c\u8a0e\u304c\u5fc5\u8981\u306b\u306a\u308b\u3053\u3068\u3082\u3042\u308a\u307e\u3059\u3002\u307e\u305a\u306f\u4e0a\u8a18\u91cf\u306b\u306a\u308b\u3088\u3046\u30a2\u30d7\u30e9\u30a4\u3057\u3001\u7d50\u679c\u3092\u898b\u3066\u304b\u3089\u6700\u9069\u91cf\u3092\u6c7a\u3081\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u4e00\u65b9\u3001\u30b2\u30eb\u306e\u539a\u3055\u3084\u30a6\u30a7\u30eb\u6570\u3054\u3068\u306b\u6700\u5927\u30b5\u30f3\u30d7\u30eb\u30a2\u30d7\u30e9\u30a4\u91cf\u304c\u6c7a\u307e\u3063\u3066\u3044\u307e\u3059\uff08\u88682\uff09\u3002<\/p>\n

\u88682\uff1a\u6700\u5927\u30b5\u30f3\u30d7\u30eb\u30a2\u30d7\u30e9\u30a4\u91cf\u306e\u76ee\u5b89<\/p>\n\n\n\n\n\n\n
\u30a6\u30a7\u30eb\u306e\u5f62\u72b6<\/b><\/td>\n\u30b2\u30eb\u306e\u539a\u3055<\/b><\/td>\n10<\/b>\u30a6\u30a7\u30eb<\/b><\/td>\n12<\/b>\u30a6\u30a7\u30eb<\/b><\/td>\n15<\/b>\u30a6\u30a7\u30eb<\/b><\/td>\n17<\/b>\u30a6\u30a7\u30eb<\/b><\/td>\n<\/tr>\n
\u4e00\u822c\u30a6\u30a7\u30eb<\/b><\/td>\n\u30001 mm<\/td>\n25 \u03bcL<\/td>\n20 \u03bcL<\/td>\n15 \u03bcL<\/td>\n15 \u03bcL<\/td>\n<\/tr>\n
1.5 mm<\/td>\n37 \u03bcL<\/td>\nn\/a<\/td>\n25 \u03bcL<\/td>\nn\/a<\/td>\n<\/tr>\n
\u304f\u3055\u3073\u578b\u30a6\u30a7\u30eb<\/b><\/td>\n\u30001 mm<\/td>\n60 \u03bcL<\/td>\n45 \u03bcL<\/td>\n35 \u03bcL<\/td>\n30 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b\u4e0a\u8a18\u306fInvitrogen\u2122 Novex\u2122\u3001NuPAGE\u2122\u3001Bolt\u2122\u30b7\u30ea\u30fc\u30ba\u306e\u30b2\u30eb\u306e\u5834\u5408\u3067\u3059\u3002\u30e1\u30fc\u30ab\u30fc\u306b\u3088\u3063\u3066\u7570\u306a\u308b\u53ef\u80fd\u6027\u304c\u3042\u308b\u305f\u3081\u3001\u3054\u4f7f\u7528\u306e\u30b2\u30eb\u306e\u30a2\u30d7\u30e9\u30a4\u91cf\u306f\u5fc5\u305a\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30bf\u30f3\u30d1\u30af\u8cea\u6fc3\u5ea6\u304c\u8584\u3044\u5834\u5408\u3001\u6fc3\u3044\u5834\u5408\u3088\u308a\u3082\u30a2\u30d7\u30e9\u30a4\u306b\u5fc5\u8981\u306a\u5bb9\u91cf\u306f\u3082\u3061\u308d\u3093\u591a\u304f\u306a\u308a\u307e\u3059\u3002\u3082\u3057\u4e00\u822c\u30a6\u30a7\u30eb\u3060\u3068\u5165\u308a\u304d\u3089\u306a\u3044\u5834\u5408\u306f\u3001\u3088\u308a\u591a\u304f\u30a2\u30d7\u30e9\u30a4\u3067\u304d\u308b1.5 mm\u539a\u304b\u3001\u304f\u3055\u3073\u578b\u30a6\u30a7\u30eb<\/b>\u306e\u30b2\u30eb\u304c\u304a\u3059\u3059\u3081\u3067\u3059\uff08\u88682\uff09\u3002\u304f\u3055\u3073\u578b\u30a6\u30a7\u30eb\u306f\u3001\u6fc3\u5ea6\u304c\u8584\u3044\u30b5\u30f3\u30d7\u30eb\u306e\u5f37\u3044\u5473\u65b9\u3067\u3001\u4e00\u822c\u30a6\u30a7\u30eb\u306e\u500d\u4ee5\u4e0a\u306e\u5bb9\u91cf\u3092\u30a2\u30d7\u30e9\u30a4\u3067\u304d\u307e\u3059\u3002\u304f\u3055\u3073\u578b\u30a6\u30a7\u30eb\u306e\u30b2\u30eb\u3092\u4f7f\u7528\u3057\u305f\u3044\u5834\u5408\u306f\u3001Invitrogen\u2122 Bolt\u2122 Bis-Tris\u30b2\u30eb<\/a>\u304b\u3001Novex\u2122 Wedgewell Tris-Glycine\u30b2\u30eb<\/a>\u3092\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/p>\n

\"Bolt

\u56f3\uff1aBolt Bis-Tris\u30b2\u30eb\u307e\u305f\u306fNovex Tris-Glycine\u30b2\u30eb\u306e\u304f\u3055\u3073\u578b\u30a6\u30a7\u30eb<\/p><\/div>\n

SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u6c7a\u3081\u65b9<\/h2>\n

\"\"
\nSDS-PAGE\u524d\u306b\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092SDS-PAGE\u304c\u3067\u304d\u308b\u72b6\u614b\u306b\u6574\u3048\u3066\u3042\u3052\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002\u3059\u306a\u308f\u3061\u3001(1) SDS\u3067\u30b5\u30f3\u30d7\u30eb\u4e2d\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u30de\u30a4\u30ca\u30b9\u306b\u30c1\u30e3\u30fc\u30b8\u3055\u305b\u3001(2) \u9084\u5143\u5264\u3067\u30b8\u30b9\u30eb\u30d5\u30a3\u30c9\u7d50\u5408\u3092\u5207\u65ad\u3057\u305f\u5f8c\u3001(3) \u71b1\u306b\u3088\u308b\u5909\u6027\u3092\u884c\u3044\u307e\u3059\u3002
\n\u7b2c\uff11\u56de<\/a>\u3067\u3054\u7d39\u4ecb\u3057\u305f\u3088\u3046\u306b\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u8a66\u85ac\u3068\u3057\u3066SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u4f7f\u7528\u3057\u3066\u3044\u308b\u5834\u5408\u306f\u3001(1)(2)\u306e\u30b9\u30c6\u30c3\u30d7\u3092\u7701\u7565\u3067\u304d\u307e\u3059\u3002\u305d\u3046\u3067\u306f\u306a\u3044\u5834\u5408\u306f\u3001\u62bd\u51fa\u3057\u305f\u30b5\u30f3\u30d7\u30eb\u3068\u3001SDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u6df7\u305c\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u3053\u3053\u3067\u4f7f\u7528\u3059\u308bSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001\u30b2\u30eb\u306b\u3088\u308a\u7570\u306a\u308a\u307e\u3059\u3002Bis-Tris\u30b2\u30eb\u3001Tris-Glycine\u30b2\u30eb\u3001Tris-Acetate\u30b2\u30eb\u306fSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc<\/a>\u3001\u307e\u305f\u306fLDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc<\/a>\u3001Tris-Tricine\u30b2\u30eb\u306f\u5c02\u7528\u306e\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc<\/a>\u3092\u4f7f\u7528\u3057\u307e\u3059\u3002<\/p>\n

\u307e\u305f\u3001\u5e02\u8ca9\u306eSDS-PAGE\u7528\u306e\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u9084\u5143\u5264\u304c\u542b\u307e\u308c\u3066\u3044\u306a\u3044\u5834\u5408\u306f\u3001\u30a2\u30d7\u30e9\u30a4\u524d\u306b\u6dfb\u52a0\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002SDS-PAGE\u3067\u306f\u3001\u9084\u5143\u5264\u3068\u3057\u3066\u30012-\u30e1\u30eb\u30ab\u30d7\u30c8\u30a8\u30bf\u30ce\u30fc\u30eb\uff082-ME\uff09\u3068DTT\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u3002<\/p>\n

\u88683\u3000SDS-PAGE\u7528\u9084\u5143\u5264<\/p>\n\n\n\n\n\n
\u9084\u5143\u5264<\/td>\n\u4e00\u822c\u7684\u306aSDS\u30b5\u30f3\u30d7\u30eb\u30d0\u30c3\u30d5\u30a1\u30fc\u4e2d\u306e\u6fc3\u5ea6<\/td>\n\u30e1\u30ea\u30c3\u30c8<\/td>\n\u30c7\u30e1\u30ea\u30c3\u30c8<\/td>\n<\/tr>\n
DTT<\/td>\n50\uff5e100 mM\u7a0b\u5ea6<\/td>\n\u9084\u5143\u529b\u304c\u5f37\u304f\u3001\u5c11\u306a\u3044\u91cf\u3067\u9ad8\u3044\u9084\u5143\u529b\u304c\u3042\u308b<\/td>\n\u7c89\u672b\u88fd\u54c1\u306e\u5834\u5408\u3001\u8abf\u6574\u306b\u624b\u9593\u304c\u304b\u304b\u308b\u203b<\/sup><\/td>\n<\/tr>\n
2-\u30e1\u30eb\u30ab\u30d7\u30c8\u30a8\u30bf\u30ce\u30fc\u30eb\uff082-ME\uff09<\/td>\n2.5\uff5e5%\u7a0b\u5ea6<\/td>\n\u6db2\u4f53\u3067\u8ca9\u58f2\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u591a\u3044\u305f\u3081\u3001\u6df7\u5408\u3057\u3084\u3059\u3044<\/td>\n\u6bd2\u7269\u304a\u3088\u3073\u5287\u7269\u53d6\u7de0\u6cd5\u306e\u5bfe\u8c61\u3068\u306a\u3063\u3066\u304a\u308a\u300120 L\u4ee5\u4e0b\u306e\u5bb9\u5668\u3067\u22660.1%\u306f\u975e\u8a72\u5f53\u3067\u3059\u304c\u3001\u226610%\u3067\u5287\u7269\u3001>10%\u3067\u6bd2\u7269\u3068\u306a\u308b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b\u3042\u3089\u304b\u3058\u3081500 mM\u306b\u8abf\u6574\u3055\u308c\u305fDTT\u3082\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\uff08\u3053\u3061\u3089<\/a>\u3068\u3053\u3061\u3089<\/a>\uff09\u3002<\/p>\n

\u30de\u30fc\u30ab\u30fc\u306e\u9078\u3073\u65b9<\/h2>\n

\"\"
\n\u300c\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u7528\u306e\u30de\u30fc\u30ab\u30fc\u300d\u3067\u691c\u7d22\u3092\u3059\u308b\u3068\u3001\u305f\u3044\u3066\u3044\u3001\u7740\u8272\u6e08\u30de\u30fc\u30ab\u30fc\u3068\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u691c\u51fa\u7528\u30de\u30fc\u30ab\u30fc\u306e2\u7a2e\u985e\u304c\u73fe\u308c\u307e\u3059\u3002\u3055\u3066\u3001\u3069\u3061\u3089\u3092\u4f7f\u7528\u3059\u308c\u3070\u3088\u3044\u3067\u3057\u3087\u3046\u304b\u3002<\/p>\n

\u7b54\u3048\u306f\u3001\u3069\u3061\u3089\u306e\u4f7f\u7528\u3082\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002
\n\u7740\u8272\u6e08\u30de\u30fc\u30ab\u30fc\u306f\u8ee2\u5199\u5f8c\u306b\u30e1\u30f3\u30d6\u30ec\u30f3\u4e0a\u3067\u3082\u76ee\u8996\u3067\u304d\u308b\u305f\u3081\u3001\u8ee2\u5199\u3055\u308c\u305f\u304b\u3069\u3046\u304b\u306e\u78ba\u8a8d\u304c\u3067\u304d\u307e\u3059\u3002\u3057\u304b\u3057\u7740\u8272\u6e08\u30de\u30fc\u30ab\u30fc\u306f\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u691c\u51fa\u3067\u304d\u306a\u3044\u305f\u3081\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u691c\u51fa\u3067\u304d\u308b\u30de\u30fc\u30ab\u30fc\u3082\u6cf3\u52d5\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002
\n\"\"
\n\u307e\u305f\u3001\u30de\u30fc\u30ab\u30fc\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf\u304c\u30ab\u30d0\u30fc\u3067\u304d\u308b\u3082\u306e\u3092\u9078\u3076\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u3002
\n\u88684\u306f\u7740\u8272\u6e08\u30de\u30fc\u30ab\u30fc\u4e00\u89a7\u3001\u88685\u306f\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u7528\u30de\u30fc\u30ab\u30fc\u306e\u4e00\u89a7\u3067\u3059\u3002\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf\u304b\u3089\u9078\u629e\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u88684\uff1a\u7740\u8272\u6e08\u30de\u30fc\u30ab\u30fc\u4e00\u89a7<\/p>\n\n\n\n\n\n\n\n\n\n\n\n\n\n
\u30de\u30fc\u30ab\u30fc<\/td>\n\u5206\u5b50\u91cf\uff08kDa\uff09<\/td>\n<\/tr>\n
Invitrogen\u2122 HiMark\u2122 Pre-stained Protein Standard<\/a><\/td>\n500, 290, 240, 160, 116, 97, 66, 55, 40<\/td>\n<\/tr>\n
Thermo Scientific\u2122 Spectra\u2122 Multicolor High Range Protein Ladder<\/a><\/td>\n300, 250, 180, 130, 100, 70, 50, 40<\/td>\n<\/tr>\n
Novex Sharp Pre-stained Protein Standard<\/a><\/td>\n260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3.5<\/td>\n<\/tr>\n
Thermo Scientific\u2122 Spectra Multicolor Broad range protein ladder<\/a><\/td>\n260, 140, 100, 70, 50, 40, 35, 25, 15, 10<\/td>\n<\/tr>\n
Thermo Scientific\u2122 PageRuler\u2122 Plus Prestained Protein Ladder, 10 to 250 kDa<\/a><\/td>\n250, 130, 100, 70, 55, 35, 25, 15, 10<\/td>\n<\/tr>\n
Invitrogen\u2122 iBright\u2122 Prestained Protein Ladder<\/a><\/td>\n250, 130, 95, 80, 70, 55, 43, 34, 30, 26, 15, 11
\n\uff08\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306780\u300130 kDa\u306e\u30d0\u30f3\u30c9\u3082\u691c\u51fa\u53ef\u80fd\uff09<\/td>\n<\/tr>\n
Invitrogen\u2122 SeeBlue\u2122 Plus2 Pre-stained Protein Standard<\/a><\/td>\n198, 98, 62, 49, 38, 28, 17, 14, 6, 3<\/td>\n<\/tr>\n
Thermo Scientific\u2122 PageRuler\u2122 Prestained Protein Ladder, 10 to 180 kDa<\/a><\/td>\n180, 130, 100, 70, 55, 40, 35, 25, 15, 10<\/td>\n<\/tr>\n
Thermo Scientific\u2122 Spectra\u2122 Multicolor Low Range Protein Ladder<\/a><\/td>\n40, 25, 15, 10, 4.6, 1.7<\/td>\n<\/tr>\n
\u305d\u306e\u4ed6\u3001\u5e02\u8ca9\u306e\u7740\u8272\u6e08\u30de\u30fc\u30ab\u30fc<\/td>\n\u5206\u5b50\u91cf\u3092\u5fc5\u305a\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u88685\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u691c\u51fa\u7528\u30de\u30fc\u30ab\u30fc\u3068\u5206\u5b50\u91cf\u4e00\u89a7<\/p>\n\n\n\n\n\n\n
\u30de\u30fc\u30ab\u30fc<\/td>\n\u5206\u5b50\u91cf\uff08kDa\uff09<\/td>\n<\/tr>\n
MagicMark XP Western Protein Standard\u3000<\/a><\/td>\n220, 120, 100, 80, 60, 50, 40, 30, 20<\/td>\n<\/tr>\n
Thermo Scientific\u2122 SuperSignal\u2122 Molecular Weight Protein Ladder<\/a><\/td>\n150, 100, 80, 60, 50, 40, 30, 20<\/td>\n<\/tr>\n
\u305d\u306e\u4ed6\u3001\u5e02\u8ca9\u306e\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u691c\u51fa\u30de\u30fc\u30ab\u30fc<\/td>\n\u5206\u5b50\u91cf\u3092\u5fc5\u305a\u78ba\u8a8d\u3057\u307e\u3057\u3087\u3046<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

 <\/p>\n

\u88685\u306e\u30de\u30fc\u30ab\u30fc\u306f\u3001\u3044\u305a\u308c\u3082\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u691c\u51fa\u3067\u304d\u308b\u30de\u30fc\u30ab\u30fc\u3067\u3059\u304c\u3001\u3069\u3061\u3089\u3082IgG\u7d50\u5408\u30b5\u30a4\u30c8\u3092\u6301\u3063\u3066\u304a\u308a\u3001\u3053\u3053\u306b\u9175\u7d20\u6a19\u8b58\u6297\u4f53\u304c\u7d50\u5408\u3059\u308b\u3053\u3068\u3067\u691c\u51fa\u3055\u308c\u307e\u3059\u3002\u3053\u306e2\u3064\u306e\u30de\u30fc\u30ab\u30fc\u306f\u6cf3\u52d5\u3059\u308b\u3060\u3051\u3067\u691c\u51fa\u304c\u3067\u304d\u308b\u7c21\u5358\u306a\u30bf\u30a4\u30d7\u3067\u3059\u304c\u3001\u5e02\u8ca9\u306e\u30de\u30fc\u30ab\u30fc\u306b\u3088\u3063\u3066\u306f\u3001\u5225\u9014\u8a66\u85ac\u304c\u5fc5\u8981\u306b\u306a\u308b\u30bf\u30a4\u30d7\u3082\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u8cfc\u5165\u524d\u306b\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u7528\u306e\u30de\u30fc\u30ab\u30fc\u306e\u691c\u51fa\u539f\u7406\u3092\u3057\u3063\u304b\u308a\u78ba\u8a8d\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u7740\u8272\u6e08\u30de\u30fc\u30ab\u30fc\u3068\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u691c\u51fa\u7528\u30de\u30fc\u30ab\u30fc\u3092\u305d\u308c\u305e\u308c\u30a2\u30d7\u30e9\u30a4\u3059\u308b\u3068\u30012\u30a6\u30a7\u30eb\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u304c\u3001\u30de\u30fc\u30ab\u30fc\u306b\u3088\u3063\u3066\u306f\u3001\u3042\u3089\u304b\u3058\u3081\u6df7\u305c\u3066\u304a\u304d\u30011\u30a6\u30a7\u30eb\u306b\u30a2\u30d7\u30e9\u30a4\u3059\u308b\u3053\u3068\u3082\u3067\u304d\u307e\u3059\u3002\uff08\u4f8b\uff1aInvitrogen\u2122 MagicMark\u2122 XP Western Protein Standard<\/a> 5 \u03bcL\u3068Novex\u2122 Sharp Pre-Stained Standard<\/a> 5 \u03bcL\u3092\u6df7\u305c\u30661\u30a6\u30a7\u30eb\u306b\u30a2\u30d7\u30e9\u30a4\u3067\u304d\u307e\u3059\u3002\u307e\u305f\u306f\u3001MagicMark XP Western Protein Standard<\/a> 10 \u03bcL\u3068\u3001Invitrogen\u2122 SeeBlue\u2122 Pre-stained Standard<\/a> 5 \u03bcL\u3092\u6df7\u305c\u30661\u30a6\u30a7\u30eb\u306b\u30a2\u30d7\u30e9\u30a4\u3082\u53ef\u80fd\u3067\u3059\uff09<\/p>\n

\u96fb\u6c17\u6cf3\u52d5\u88c5\u7f6e\u3068\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\u306e\u9078\u629e<\/h2>\n

\"\"
\n\u6cf3\u52d5\u306e\u4e3b\u5f79\u306b\u306a\u308b\u306e\u306f\u30b5\u30f3\u30d7\u30eb\u3084\u30b2\u30eb\u3067\u3059\u304c\u3001\u821e\u53f0\u3082\u5fc5\u8981\u3067\u3059\u3002\u3064\u307e\u308a\u3001\u6cf3\u52d5\u88c5\u7f6e\u3068\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\u3082\u7528\u610f\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002
\n\u307e\u305a\u3001\u9078\u3093\u3060\u30b2\u30eb\u304c\u6cf3\u52d5\u3067\u304d\u308b\u88c5\u7f6e\u304c\u5fc5\u8981\u3067\u3059\u3002\u88681\u306e\u30b2\u30eb\u306f\u3059\u3079\u3066Invitrogen\u2122 Bolt\u2122 Mini Gel Tank<\/a>\u307e\u305f\u306fXCell SureLock\u2122 Mini-Cell<\/a>\u3067\u6cf3\u52d5\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u88686\u3000\u30df\u30cb\u30b2\u30eb\u7528\u6cf3\u52d5\u88c5\u7f6e<\/p>\n\n\n\n\n\n
\u96fb\u6c17\u6cf3\u52d5\u88c5\u7f6e<\/td>\n\u5199\u771f<\/td>\n\u6700\u5927\u6cf3\u52d5\u679a\u6570<\/td>\n\u30e1\u30ea\u30c3\u30c8<\/td>\n<\/tr>\n
Bolt Mini Gel Tank<\/a><\/td>\n\"\"<\/td>\n2\u679a<\/td>\n\u30fb2\u679a\u3092\u6a2a\u306b\u4e26\u3079\u3066\u6cf3\u52d5\u3059\u308b\u305f\u3081\u3001\u6cf3\u52d5\u4e2d\u306b\u30b2\u30eb\u304c\u78ba\u8a8d\u3057\u3084\u3059\u3044<\/p>\n

\u30fb\u30d0\u30c3\u30d5\u30a1\u30fc\u30bf\u30f3\u30af\u306f\u8ee2\u5199\u306b\u3082\u5229\u7528\u53ef\u80fd<\/td>\n<\/tr>\n

XCell SureLock Mini-Cell<\/a><\/td>\n\"\"<\/td>\n2\u679a<\/td>\n\u30fbMini Gel Tank\u306b\u6bd4\u3079\u3066\u8ad6\u6587\u306a\u3069\u306e\u5b9f\u7e3e\u304c\u591a\u3044<\/p>\n

\u30fb\u30d0\u30c3\u30d5\u30a1\u30fc\u30bf\u30f3\u30af\u306f\u8ee2\u5199\u306b\u3082\u5229\u7528\u53ef\u80fd<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u6b21\u306b\u3001\u3053\u308c\u3089\u306e\u6cf3\u52d5\u88c5\u7f6e\u3067\u6cf3\u52d5\u3059\u308b\u305f\u3081\u306b\u306f\u3001\u96fb\u6e90\u3001\u3064\u307e\u308a\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\u304c\u5fc5\u8981\u306b\u306a\u308a\u307e\u3059\u3002\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4\u306f\u3001\u4f7f\u7528\u3057\u305f\u3044\u30b2\u30eb\u306e\u6cf3\u52d5\u6761\u4ef6\uff08\u96fb\u5727\u5024\u3084\u96fb\u6d41\u5024\uff09\u304c\u51fa\u529b\u3067\u304d\u308b\u3082\u306e\u3092\u7528\u610f\u3057\u307e\u3057\u3087\u3046\u3002\u6cf3\u52d5\u6761\u4ef6\u306f\u30b2\u30eb\u3054\u3068\u306b\u7570\u306a\u308a\u307e\u3059\u306e\u3067\u3001\u3042\u3089\u304b\u3058\u3081\u30b2\u30eb\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u304b\u3089\u306e\u78ba\u8a8d\u3092\u304a\u3059\u3059\u3081\u3057\u307e\u3059\u3002
\nMini Gel Tank\u3068XCell SureLock Mini-Cell\u63a8\u5968\u306e\u96fb\u6e90\u306f\u3001Invitrogen\u2122 PowerEase\u2122\u30b7\u30ea\u30fc\u30ba\u3067\u3059\u3002\u305d\u308c\u305e\u308c\u6700\u5927\u96fb\u5727\u3001\u96fb\u6d41\u5024\u304c\u7570\u306a\u308b\u305f\u3081\u3001\u4f7f\u7528\u3067\u304d\u308b\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3084\u30b2\u30eb\u679a\u6570\u306b\u9055\u3044\u304c\u3042\u308a\u307e\u3059\uff08\u88686\uff09\u3002<\/p>\n

\u88687\u3000PowerEase\u30b7\u30ea\u30fc\u30ba\u3068\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3054\u3068\u306e\u4f7f\u3044\u5206\u3051<\/p>\n\n\n\n\n\n\n
\u30d1\u30ef\u30fc\u30b5\u30d7\u30e9\u30a4<\/td>\n\u6700\u5927\u51fa\u529b\u5024\uff08\u96fb\u5727\u3001\u96fb\u6d41\u3001\u96fb\u529b\uff09<\/td>\n\u63a8\u5968\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u3068\u30b2\u30eb\u679a\u6570\u306e\u76ee\u5b89<\/td>\n<\/tr>\n
Invitrogen\u2122 PowerEase\u2122 Touch 120W Power Supply<\/a><\/td>\n300 V\u3001500 mA\u3001120 W<\/td>\nSDS-PAGE\uff1a\u30df\u30cb\u30b2\u30eb1~2\u679a
\n\u8ee2\u5199\uff1a\u30df\u30cb\u30b2\u30eb1\u679a\u7a0b\u5ea6<\/td>\n<\/tr>\n
Invitrogen\u2122 PowerEase\u2122 Touch 350W Power Supply<\/a><\/td>\n300 V\u30013A\u3001350 W<\/td>\nSDS-PAGE\uff1a\u30df\u30cb\u30b2\u30eb1\uff5e4\u679a
\n\u8ee2\u5199\uff1a\u30df\u30cb\u30b2\u30eb1~4\u679a<\/td>\n<\/tr>\n
Invitrogen\u2122 PowerEase\u2122 Touch 600W Power Supply<\/a><\/td>\n500 V\u30013A\u3001600 W<\/td>\nSDS-PAGE\uff1a\u30df\u30cb\u30b2\u30eb1\uff5e4\u679a
\n\u7b49\u96fb\u70b9\u96fb\u6c17\u6cf3\u52d5\u203b<\/sup>\uff1a\u30df\u30cb\u30b2\u30eb1\uff5e4\u679a
\n\u8ee2\u5199\uff1a\u30df\u30cb\u30b2\u30eb1~4\u679a<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b\u4e8c\u6b21\u5143\u96fb\u6c17\u6cf3\u52d5\u306e\u4e00\u6b21\u5143\u76ee\u306e\u7b49\u96fb\u70b9\u96fb\u6c17\u6cf3\u52d5\u306b\u306f\u304a\u3059\u3059\u3081\u3067\u304d\u307e\u305b\u3093\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002\u3053\u306e\u5834\u5408\u306f\u3001Invitrogen\u2122 ZOOM\u2122 Dual Power Supply\u304c\u304a\u3059\u3059\u3081\u3067\u3059\u3002<\/p>\n

\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u305f\u3081\u306eSDS-PAGE\u306e\u9078\u3073\u65b9<\/h2>\n

SDS-PAGE\u306e\u30b2\u30eb\u3084\u30d0\u30c3\u30d5\u30a1\u30fc\u3001\u30de\u30fc\u30ab\u30fc\u306b\u306f\u3055\u307e\u3056\u307e\u306a\u7a2e\u985e\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u30dd\u30a4\u30f3\u30c8\u3092\u7d5e\u3063\u3066\u9078\u3093\u3067\u3044\u3051\u3070\u8ff7\u3046\u3053\u3068\u3082\u5c11\u306a\u3044\u306f\u305a\u3067\u3059\u3002\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u5408\u3063\u305f\u3082\u306e\u3092\u3001\u3057\u3063\u304b\u308a\u3068\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u6b21\u56de<\/a>\u306f\u3001SDS-PAGE\u3067\u5206\u96e2\u3057\u305f\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u3001\u30b2\u30eb\u3088\u308a\u3082\u4e08\u592b\u306a\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u79fb\u3059\u4f5c\u696d\u3001\u3064\u307e\u308a\u8ee2\u5199\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u30b7\u30ea\u30fc\u30ba\u306f\u3053\u3061\u3089\uff1a
\n\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c1\u56de\uff1a\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u62bd\u51fa\u65b9\u6cd5<\/a>
\n\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c2\u56de\uff1a\u7dcf\u30bf\u30f3\u30d1\u30af\u8cea\u5b9a\u91cf\u30ad\u30c3\u30c8<\/a>
\nSDS-PAGE\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c3\u56de
\n\u8ee2\u5199\u6761\u4ef6\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c4\u56de<\/a>
\n\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c5\u56de<\/a>
\n\u6297\u4f53\u3092\u9078\u3076\u3068\u304d\u306b\u77e5\u3063\u3066\u304a\u304d\u305f\u3044\u3053\u3068\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u7b2c6\u56de<\/a>
\n\u5316\u5b66\u767a\u5149\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306e\u9078\u629e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u3000\u7b2c7\u56de<\/a><\/p>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n

\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

 <\/p>\n

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