{"id":13391,"date":"2023-02-22T09:10:07","date_gmt":"2023-02-22T00:10:07","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=13391"},"modified":"2023-12-27T15:55:28","modified_gmt":"2023-12-27T06:55:28","slug":"elisa-workshop-bid-ts-4","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/elisa-workshop-bid-ts-4\/","title":{"rendered":"\u3010ELISA\u3084\u3063\u3066\u307f\u305f\u3011Detector Antibody\u306e\u6fc3\u5ea6\u3092\u5909\u3048\u305f\u3089\u3069\u3046\u306a\u308b\u306e\uff1f"},"content":{"rendered":"<p>\u30b5\u30f3\u30c9\u30a4\u30c3\u30c1ELISA\u306e\u7cfb\u3092\u81ea\u4f5c\u3067\u69cb\u7bc9\u3059\u308b\u5834\u5408\u306b\u306f\u3001\u6297\u4f53\u30da\u30a2\u306e\u9078\u5b9a\u3001\u691c\u91cf\u7dda\u7528\u306e\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u30bf\u30f3\u30d1\u30af\u306e\u9078\u5b9a\u3001\u6297\u4f53\u6fc3\u5ea6\u3001\u53cd\u5fdc\u6642\u9593\u3001\u30b5\u30f3\u30d7\u30eb\u8abf\u6574\u2026\u3068\u6570\u591a\u304f\u306e\u6761\u4ef6\u691c\u8a0e\u304c\u5fc5\u8981\u3067\u3059\u3002\u30b9\u30c6\u30c3\u30d7\u6570\u3082\u591a\u3044\u306e\u3067\u3001\u4f55\u3092\u3069\u3046\u6761\u4ef6\u691c\u8a0e\u3059\u308c\u3070\u3088\u3044\u304b\u60a9\u3080\u65b9\u3082\u5c11\u306a\u304f\u3042\u308a\u307e\u305b\u3093\u3002<\/p>\n<p>\u3053\u3061\u3089\u306e<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/elisa-workshop-bid-ts-3\/?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_elisa_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010ELISA\u3084\u3063\u3066\u307f\u305f\u3011\u3067\u306fCapture Antibody\u306e\u6fc3\u5ea6\u3092\u691c\u8a0e<\/a>\u3057\u307e\u3057\u305f\u304c\u3001\u4eca\u56de\u306fDetector Antibody\uff08\u691c\u51fa\u6297\u4f53\uff09\u306b\u30d5\u30a9\u30fc\u30ab\u30b9\u3057\u3001\u6297\u4f53\u6fc3\u5ea6\u3092\u5909\u66f4\u3059\u308b\u3068\u7d50\u679c\u304c\u3069\u3046\u5909\u5316\u3059\u308b\u304b\u3092\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<p>\u25bc\u3053\u3093\u306a\u65b9\u306b\u304a\u3059\u3059\u3081\u3067\u3059\uff01<br \/>\n\u30fb\u3053\u308c\u304b\u3089\u81ea\u4f5cELISA\u3092\u69cb\u7bc9\u3057\u305f\u3044<br \/>\n\u30fbELISA\u306e\u6761\u4ef6\u691c\u8a0e\u3067\u60a9\u3093\u3067\u3044\u308b<\/p>\n<h2>\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n<p>\u6750\u6599\uff1a<br \/>\nInvitrogen\u2122<a href=\"https:\/\/www.thermofisher.com\/elisa\/product\/IL-6-Mouse-Uncoated-ELISA-Kit-with-Plates\/88-7064-86?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_elisa_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">IL-6 Mouse Uncoated ELISA Kit with Plates \uff0888-7064-88\uff09<\/a><\/p>\n<p>\u65b9\u6cd5\uff1a<br \/>\n\u30ad\u30c3\u30c8\u306b\u542b\u307e\u308c\u308bDetector Antibody\u306b\u3064\u3044\u3066\u3001\u30de\u30cb\u30e5\u30a2\u30eb\u63a8\u5968\u306e\u5e0c\u91c8\u7387\u304cx250\u306e\u3068\u3053\u308d\u30016\u7a2e\u985e\u306e\u5e0c\u91c8\u7387\u3067\u8abf\u88fd\u3057\u307e\u3057\u305f\u3002<br \/>\nDetector Antibody\u6fc3\u5ea6\u304c\u9ad8\u3044\u65b9\u304b\u3089\u9806\u306bx62.5\u3001x 125\u3001x 250\u3001x 500\u3001x 1000\u3001x 2000<\/p>\n<p>\u30ad\u30c3\u30c8\u4ed8\u5c5e\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u306b\u5f93\u3044\u4f5c\u696d\u3092\u884c\u3044\u307e\u3057\u305f\u3002<br \/>\n\u691c\u91cf\u7dda\u7528\u306e\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u30b5\u30f3\u30d7\u30eb\uff087\u6bb5\u968e\uff09\u3092\u53cd\u5fdc\u3055\u305b\u3001\u6d17\u6d44\u3001\u305d\u306e\u5f8c\u306e\u53cd\u5fdc\u30b9\u30c6\u30c3\u30d7\u3092\u5b9f\u65bd\u3057\u307e\u3057\u305f\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/lab-equipment\/microplate-instruments\/plate-readers\/models\/multiskan-skyhigh.html?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_elisa_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">Thermo Scientific\u2122 Multiskan\u2122 SkyHigh Microplate Spectrophotometer<\/a>\u3067450 nm\u306e\u5438\u5149\u5ea6\u3092\u6e2c\u5b9a\u3057\u3001Thermo Scientific\u2122 SkanIt\u2122\u30bd\u30d5\u30c8\u30a6\u30a8\u30a2\u3067\u89e3\u6790\u3057\u30014PL\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3057\u307e\u3057\u305f\u3002<\/p>\n<h2>\u7d50\u679c<\/h2>\n<p>\u5404\u5e0c\u91c8\u7387\u306b\u304a\u3051\u308b\u6e2c\u5b9a\u5024\u3092\u5229\u7528\u3057\u3001\u4f5c\u6210\u3055\u308c\u305f\u691c\u91cf\u7dda\u306f\u4e0b\u8a18\u306b\u306a\u308a\u307e\u3059\u3002\u6297\u4f53\u6fc3\u5ea6\u4f9d\u5b58\u7684\u306b\u5438\u5149\u5ea6\u304c\u5909\u52d5\u3059\u308b\u7d50\u679c\u3068\u306a\u308a\u307e\u3057\u305f\u3002<br \/>\n<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-13392\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/02\/3164ba3a77b7d843547e214c5dc7d55a.png\" alt=\"\" width=\"643\" height=\"354\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/02\/3164ba3a77b7d843547e214c5dc7d55a.png 643w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/02\/3164ba3a77b7d843547e214c5dc7d55a-300x165.png 300w\" sizes=\"auto, (max-width: 643px) 100vw, 643px\" \/><br \/>\n\u6ce8\u610f\uff1a\u3053\u3061\u3089\u306fDetector Antibody\u6fc3\u5ea6\u4ee5\u5916\u306f\u5341\u5206\u306b\u691c\u8a0e\u3055\u308c\u305f\u6761\u4ef6\u3067\u5f97\u3089\u308c\u305f\u7d50\u679c\u3067\u3059\u3002<br \/>\n\u305f\u3060\u3057\u30a2\u30c3\u30bb\u30a4\u7cfb\u306b\u3088\u3063\u3066\u306f\u3001\u7d50\u679c\u306f\u5909\u308f\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n<h2>\u8003\u5bdf<\/h2>\n<p>Detector Antibody\u6fc3\u5ea6\u3092\u4e0a\u3052\u308c\u3070\u5438\u5149\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u3001\u307e\u305fDetector Antibody\u6fc3\u5ea6\u3092\u4e0b\u3052\u308c\u3070\u5438\u5149\u5ea6\u306f\u4f4e\u304f\u306a\u308b\u3001\u3068\u3044\u3046\u4e88\u60f3\u901a\u308a\u306e\u7d50\u679c\u3067\u3057\u305f\u3002\u305f\u3060x 62.5\u3068x 125\u306e\u691c\u91cf\u7dda\u3067\u306f\u9ad8\u6fc3\u5ea6\u30b5\u30f3\u30d7\u30eb\u306e\u5438\u5149\u5ea6\u304c\u975e\u5e38\u306b\u9ad8\u304f\u3001\u307e\u305fx 2000\u306e\u691c\u91cf\u7dda\u306e\u50be\u304d\u306f\u975e\u5e38\u306b\u5c0f\u3055\u3044\u3053\u3068\u304c\u6c17\u306b\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u5834\u5408\u3001\u6b21\u306e2\u70b9\u306b\u7740\u76ee\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n<p>1\uff09 \u30a2\u30c3\u30bb\u30a4\u30a6\u30a4\u30f3\u30c9\u30a6\u306e\u5e83\u3055\u306f\u5341\u5206\u304b\uff1f<br \/>\nELISA\u306f\u624b\u6280\u306b\u3088\u308b\u7d50\u679c\u306e\u5024\u306e\u3070\u3089\u3064\u304d\u304c\u751f\u3058\u3084\u3059\u3044\u5b9f\u9a13\u7cfb\u3067\u3059\u3002\u30a2\u30c3\u30bb\u30a4\u30a6\u30a4\u30f3\u30c9\u30a6\u304c\u72ed\u3044\u3068\u3001\u4e9b\u7d30\u306a\u5438\u5149\u5ea6\u306e\u3070\u3089\u3064\u304d\u304c\u3001\u691c\u91cf\u7dda\u3088\u308a\u6fc3\u5ea6\u306b\u63db\u7b97\u3057\u305f\u5834\u5408\u306b\u5927\u304d\u306a\u3070\u3089\u3064\u304d\u3068\u306a\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3067\u304d\u308b\u3060\u3051\u5e83\u3044\u30a2\u30c3\u30bb\u30a4\u30a6\u30a4\u30f3\u30c9\u30a6\u3092\u3068\u308c\u308b\u3088\u3046\u3001\u6761\u4ef6\u691c\u8a0e\u3059\u308b\u3053\u3068\u304c\u671b\u307e\u3057\u3044\u3067\u3059\u3002<\/p>\n<p>2\uff09 \u4fe1\u983c\u3067\u304d\u308b\u5438\u5149\u5ea6\u306e\u5024\u306b\u3064\u3044\u3066<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/lab-equipment\/microplate-instruments\/plate-readers\/models\/multiskan-skyhigh.html?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_elisa_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">Multiskan SkyHigh Microplate Spectrophotometer<\/a>\u3067\u306f\u3001Abs 0\uff5e2.5\u304c\u4fe1\u983c\u6027\u306e\u3042\u308b\u5438\u5149\u5ea6\u30ec\u30f3\u30b8\u306b\u306a\u308a\u307e\u3059\u3002\u4fe1\u983c\u6027\u306e\u3042\u308b\u5438\u5149\u5ea6\u30ec\u30f3\u30b8\u306f\u3054\u4f7f\u7528\u306b\u306a\u308b\u30d7\u30ec\u30fc\u30c8\u30ea\u30fc\u30c0\u30fc\u306b\u3088\u3063\u3066\u3082\u7570\u306a\u308a\u307e\u3059\u304c\u3001Abs 2\u4ee5\u4e0a\u3067\u306f\u900f\u904e\u7387\u304c1\uff05\u4ee5\u4e0b\u306b\u306a\u308b\u305f\u3081\u3001\u5fae\u5f31\u306a\u30b7\u30b0\u30ca\u30eb\u3092\u691c\u51fa\u3059\u308b\u3053\u3068\u306b\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u4e00\u822c\u7684\u306b\u306fAbs 2\u307e\u3067\u306e\u7bc4\u56f2\u3067\u6e2c\u5b9a\u3059\u308b\u3053\u3068\u304c\u63a8\u5968\u3055\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3082\u3061\u308d\u3093\u691c\u91cf\u7dda\u3092\u8a55\u4fa1\u3059\u308b\u6307\u6a19\u306f\u4e0a\u8a18\u306b\u9650\u308a\u307e\u305b\u3093\u3002\u305f\u3060\u4eca\u56de\u306e\u5b9f\u9a13\u306b\u304a\u3044\u3066\u306f\u3001x 62.5\u3068x 125\u306e\u691c\u91cf\u7dda\u306b\u304a\u3044\u3066\u3001\u5438\u5149\u5ea6\u304c2.5\u3092\u8d85\u3048\u3066\u3044\u308b\u30dd\u30a4\u30f3\u30c8\u306b\u3064\u3044\u3066\u306f\u691c\u91cf\u7dda\u306b\u63a1\u7528\u3059\u308b\u306e\u306f\u671b\u307e\u3057\u304f\u306a\u3044\u3068\u8a00\u3048\u307e\u3059\u3002<br \/>\n\u307e\u305fx 2000\u306e\u691c\u91cf\u7dda\u306b\u304a\u3044\u3066\u306f\u3001\u6539\u3081\u3066\u30ec\u30d7\u30ea\u30b1\u30fc\u30c8\u3092\u3068\u3063\u3066\u5024\u306e\u3070\u3089\u3064\u304d\u306e\u5177\u5408\u3092\u78ba\u8a8d\u3057\u3001\u4fe1\u983c\u3067\u304d\u308b\u691c\u91cf\u7dda\u304b\u3092\u518d\u8a55\u4fa1\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u3067\u3057\u3087\u3046\u3002<\/p>\n<p>\u307e\u305f\u4eca\u56de\u306e\u7d50\u679c\u3067\u306f\u793a\u3055\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001Detector Antibody\u6fc3\u5ea6\u304c\u9ad8\u3059\u304e\u308b\u3068\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u4e0a\u6607\u306e\u539f\u56e0\u306b\u3082\u306a\u308b\u3068\u8a00\u308f\u308c\u3066\u304a\u308a\u307e\u3059\u3002ELISA\u306e\u5b9f\u9a13\u7cfb\u3092\u69cb\u7bc9\u3059\u308b\u5834\u5408\u306f\u3001\u6297\u4f53\u6fc3\u5ea6\u306e\u691c\u8a0e\u304c\u91cd\u8981\u3067\u3042\u308b\u3053\u3068\u3092\u3054\u7406\u89e3\u3044\u305f\u3060\u3051\u305f\u304b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<ul>\n<li>Detector Antibody\u306e\u6fc3\u5ea6\u3092\u5909\u3048\u308b\u3068\u3001\u6fc3\u5ea6\u4f9d\u5b58\u7684\u306b\u7d50\u679c\u306e\u5438\u5149\u5ea6\u3082\u5909\u52d5\u3059\u308b<\/li>\n<li>\u30a2\u30c3\u30bb\u30a4\u30a6\u30a4\u30f3\u30c9\u30a6\u304c\u5341\u5206\u5e83\u3044\u304b\u3082\u8981\u30c1\u30a7\u30c3\u30af<\/li>\n<li>\u691c\u91cf\u7dda\u3092\u4f5c\u6210\u3059\u308b\u5834\u5408\u3001\u30b9\u30bf\u30f3\u30c0\u30fc\u30c9\u306e\u5438\u5149\u5ea6\u304c\uff5e2.5\u306b\u53ce\u307e\u308b\u3088\u3046\u6761\u4ef6\u691c\u8a0e\u3092\u884c\u3046<\/li>\n<\/ul>\n<p>\u5f0a\u793e\u3067\u306fELISA\u3060\u3051\u306a\u304f\u3001\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3084\u7d30\u80de\u57f9\u990a\u3001\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306a\u3069\u3001\u5b9f\u969b\u306b\u5b9f\u9a13\uff08\u5b9f\u7fd2\uff09\u3092\u884c\u3044\u3064\u3064\u5b66\u3079\u308b<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/lifescience-training.html?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_elisa_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" 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