{"id":1342,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_41_3_cts\/"},"modified":"2023-12-28T14:07:02","modified_gmt":"2023-12-28T05:07:02","slug":"user_voice_41_3_cts","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_41_3_cts\/","title":{"rendered":"\u518d\u751f\u533b\u7642\u306b\u7528\u3044\u3089\u308c\u308b\u9593\u8449\u7cfb\u5e79\u7d30\u80de\u306e\u8cea\u306e\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u306e\u305f\u3081\u306b\uff08\u6771\u4eac\u533b\u79d1\u6b6f\u79d1\u5927\u5b66 \u8edf\u9aa8\u518d\u751f\u5b66\u8b1b\u5ea7 \u8fbb\u90a6\u548c\u6c0f\u3001\u904b\u52d5\u5668\u5916\u79d1\u5b66 \u5c3e\u5cf6\u7f8e\u4ee3\u5b50\u6c0f\uff09-\u300cNEXT\u300d2017\u5e747\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2 style=\"text-align: left\"><span style=\"font-size: 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TrypLE\u2122 Select Enzyme\u306e\u6709\u7528\u6027\u306b\u3064\u3044\u3066\u4f3a\u3044\u307e\u3057\u305f\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u8868\u9762\u30de\u30fc\u30ab\u30fc\u304c\u5206\u89e3\u3055\u308c\u308b?<\/span><\/h2>\n<p>\u7d30\u80de\u5265\u96e2\u5264\u306b\u3064\u3044\u3066\u6bd4\u8f03\u691c\u8a0e\u3057\u3088\u3046\u3068\u8003\u3048\u305f\u304d\u3063\u304b\u3051\u306b\u3064\u3044\u3066\u8fbb\u6c0f\u306f\u3053\u3046\u8a71\u3057\u307e\u3059\u3002\u300ciPS\u7d30\u80de\u306a\u3069\u306e\u5e79\u7d30\u80de\u30d9\u30fc\u30b9\u306e\u518d\u751f\u533b\u7642\u7814\u7a76\u306f\u975e\u5e38\u306b\u76db\u3093\u3067\u3001\u5fdc\u7528\u3092\u76ee\u6307\u3057\u305f\u81a8\u5927\u306a\u77e5\u898b\u304c\u96c6\u7a4d\u3059\u308b\u4e00\u65b9\u3001\u79fb\u690d\u3059\u308b\u7d30\u80de\u306e\u8cea\u306b\u95a2\u308f\u308b\u57fa\u790e\u7684\u77e5\u898b\u306e\u84c4\u7a4d\u306f\u307e\u3060\u5341\u5206\u3067\u3042\u308a\u307e\u305b\u3093\u3002\u7279\u306bMSC\u306f\u9020\u8840\u7cfb\u7d30\u80de\u3068\u9055\u3044\u63a5\u7740\u6027\u3067\u3042\u308b\u305f\u3081\u3001\u79fb\u690d\u6642\u306b\u306f\u3001\u57f9\u990a\u76bf\u304b\u3089\u5265\u96e2\u3059\u308b\u64cd\u4f5c\u304c\u5fc5\u8981\u3068\u306a\u308a\u307e\u3059\u3002\u3053\u306e\u7d30\u80de\u61f8\u6fc1\u6db2\u8abf\u88fd\u64cd\u4f5c\u304c\u3001\u7d30\u80de\u306e\u751f\u5b58\u7387\u3084\u5206\u5316\u80fd\u7b49\u306e&#8221;\u8cea&#8221;\u306b\u5f71\u97ff\u3057\u3046\u308b\u3068\u8003\u3048\u307e\u3057\u305f\u300d\u3002\u307e\u305f\u5b9f\u9a13\u30c7\u30fc\u30bf\u304b\u3089\u3001\u8208\u5473\u6df1\u3044\u6c17\u3065\u304d\u304c\u3042\u3063\u305f\u3068\u3044\u3044\u307e\u3059\u3002\u300c2\u5e74\u534a\u307b\u3069\u524d\u304b\u3089\u54c1\u8cea\u30c1\u30a7\u30c3\u30af\u306e\u305f\u3081\u306b\u8abf\u88fd\u3057\u305f\u6ed1\u819c\u5e79\u7d30\u80de\u306e\u89e3\u6790\u3092\u59cb\u3081\u307e\u3057\u305f\u3002MSC\u306e\u4ee3\u8868\u7684\u8868\u9762\u30de\u30fc\u30ab\u30fc\u306fCD73\u3001CD90\u3001CD105\u3067\u3059\u304c\u3001\u5b9f\u9a13\u8005\u306b\u3088\u3063\u3066\u3053\u306e\u967d\u6027\u7387\u304c\u3070\u3089\u3064\u304f\u5b9f\u611f\u304c\u3042\u308a\u307e\u3057\u305f\u3002\u7279\u306b\u4e0d\u6163\u308c\u306a\u5b9f\u9a13\u8005\u306e\u5834\u5408CD105\u306e\u967d\u6027\u7387\u306b\u5e45\u304c\u751f\u3058\u308b\u306e\u3067\u3059\u300d\u3002\u3053\u306e\u3068\u304d\u30c8\u30ea\u30d7\u30b7\u30f3\u51e6\u7406\u6642\u9593\u306e\u5f71\u97ff\u3092\u76f4\u611f\u3057\u305f\u8fbb\u6c0f\u3002\u60a3\u8005\u7d44\u7e54\u7531\u6765\u306e\u521d\u4ee3\u57f9\u990a\u7d30\u80de\u304b\u3089\u6cbb\u7642\u306b\u5fc5\u8981\u306a\u7d30\u80de\u6570\u3092\u78ba\u4fdd\u3059\u308b\u306b\u306f\u3001\u6570\u5341\u679a\u306e\u30c7\u30a3\u30c3\u30b7\u30e5\u3092\u7528\u3044\u3066\u5927\u91cf\u57f9\u990a\u3059\u308b\u5fc5\u8981\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u51e6\u7406\u3059\u308b\u30c7\u30a3\u30c3\u30b7\u30e5\u306e\u679a\u6570\u304c\u591a\u304f\u306a\u308b\u307b\u3069\u7d30\u80de\u61f8\u6fc1\u6db2\u306e\u8abf\u88fd\u306b\u6642\u9593\u7684\u306a\u4e0d\u78ba\u5b9f\u6027\u304c\u751f\u3058\u307e\u3059\u3002\u3064\u307e\u308a\u5c06\u6765\u81e8\u5e8a\u5fdc\u7528\u3059\u308b\u5834\u5408\u306b\u3082\u3001\u540c\u69d8\u306e\u4e0d\u5b89\u5b9a\u3055\u304c\u751f\u3058\u308b\u3060\u308d\u3046\u3068\u8003\u3048\u307e\u3057\u305f\u3002\u300c\u4eca\u56de\u306f\u7d30\u80de\u5265\u96e2\u306e\u30b9\u30c6\u30c3\u30d7\u304c\u8868\u9762\u6297\u539f\u3084\u751f\u7d30\u80de\u7387\u3001\u5e79\u7d30\u80de\u6027\u306e\u7dad\u6301\u306b\u3069\u306e\u3088\u3046\u306a\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u304b\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u3068TrypLE\u3092\u6bd4\u8f03\u3057\u307e\u3057\u305f\u300d\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u518d\u751f\u533b\u7642\u306e\u54c1\u8cea\u3092\u62c5\u4fdd\u3059\u308b<\/span><\/h2>\n<p>\u6bd4\u8f03\u306e\u7d50\u679c\u306f\u660e\u3089\u304b\u3067\u3057\u305f\u3002\u300c\u51e6\u7406\u6642\u95935\u5206\u300130\u5206\u300160\u5206\u3067CD73\u3001CD90\u3001CD105\u3092\u542b\u308018\u7a2e\u985e\u306e\u9593\u8449\u7cfb\u5e79\u7d30\u80de\u8868\u9762\u767a\u73fe\u306e\u967d\u6027\u7387\u306b\u53ca\u307c\u3059\u5f71\u97ff\u3092\u3001\u30d5\u30ed\u30fc\u30b5\u30a4\u30c8\u30e1\u30fc\u30bf\u30fc\u3067\u89e3\u6790\u3057\u307e\u3057\u305f\u3002\u51e6\u7406\u6642\u959330\u5206\u4ee5\u5185\u306b\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u3067\u306f3\u7a2e\u985e\u306e\u30de\u30fc\u30ab\u30fc\u306e\u967d\u6027\u7387\u304c\u6709\u610f\u306b\u4f4e\u4e0b\u3057\u305f\u4e00\u65b9\u3001TrypLE\u3067\u306f5\u5206\u306830\u5206\u3067\u5927\u304d\u306a\u5dee\u304c\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u7279\u306b\u3001\u6ce8\u76ee\u3057\u3066\u3044\u305fCD105\u306e\u5834\u5408\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u3067\u306f\u7d4c\u6642\u7684\u306b\u967d\u6027\u7387\u304c\u4e0b\u304c\u308a60\u5206\u5f8c\u306b\u306f10%\u672a\u6e80\u307e\u3067\u4e0b\u304c\u308b\u4e00\u65b9\u3067\u3001TrypLE\u3067\u306f60\u5206\u5f8c\u308270%\u4ee5\u4e0a\u306e\u967d\u6027\u7387\u3092\u7dad\u6301\u3057\u307e\u3057\u305f\u3002\u307e\u305f60\u5206\u4ee5\u5185\u306a\u3089\u30d0\u30a4\u30a2\u30d3\u30ea\u30c6\u30a3\u3078\u306e\u5f71\u97ff\u306f\u3069\u3061\u3089\u3082\u5927\u5dee\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u3055\u3089\u306b\u30c8\u30ea\u30d7\u30b7\u30f3\u306f\u30015\u5206\u9593\u306e\u51e6\u7406\u3067\u3042\u3063\u3066\u3082CD140a\u967d\u6027\u7387\u3092\u8457\u3057\u304f\u6e1b\u5c11\u3055\u305b\u307e\u3057\u305f\u3002CD140a\u306fMSC\u306e\u5897\u6b96\u3001\u5206\u5316\u306b\u91cd\u8981\u306a\u6a5f\u80fd\u3092\u6709\u3059\u308bPDGF\uff08\u8840\u5c0f\u677f\u7531\u6765\u5897\u6b96\u56e0\u5b50\uff09\u306e\u53d7\u5bb9\u4f53\u3067\u3042\u308a\u3001\u3053\u306e\u7d50\u679c\u306f\u975e\u5e38\u306b\u91cd\u8981\u3067\u3042\u308b\u3068\u8003\u3048\u3066\u304a\u308a\u307e\u3059\u300d\u3002<span style=\"font-size: 8pt\">\uff08K. Tsuji, M. Ojima et al. Cell Transplantation, Vol. 26, pp. 1089\u20131102, 2017\uff09<\/span><\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-3.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3795 size-medium\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-3-300x125.jpg\" alt=\"\u9593\u8449\u7cfb\u5e79\u7d30\u80de\" width=\"300\" height=\"125\" \/><\/a>TrypLE\u306e\u4f7f\u7528\u611f\u306b\u3064\u3044\u3066\u5c3e\u5cf6\u6c0f\u306f\u300c\u6700\u3082\u30dd\u30d4\u30e5\u30e9\u30fc\u306a\u30c8\u30ea\u30d7\u30b7\u30f3\u306f\u5f37\u529b\u3067\u3059\u304c\u3001MSC\u30de\u30fc\u30ab\u30fc\u967d\u6027\u7387\u306e\u4f4e\u4e0b\u304c\u660e\u3089\u304b\u3068\u306a\u308a\u307e\u3057\u305f\u3002\u6642\u9593\u3068\u5171\u306b\u8868\u9762\u30de\u30fc\u30ab\u30fc\u306f\u30ea\u30ab\u30d0\u30ea\u30fc\u3055\u308c\u308b\u3068\u306f\u3044\u3048\u3001\u7d30\u80de\u306e\u8cea\u7684\u5909\u5316\u3082\u3042\u308a\u5f97\u307e\u3059\u3002TrypLE\u306e\u52b9\u304d\u65b9\u306f\u3088\u308a\u30de\u30a4\u30eb\u30c9\u3067\u3001\u51e6\u7406\u6642\u9593\u304c5\u5206\u3067\u308230\u5206\u3067\u3082\u8868\u9762\u30de\u30fc\u30ab\u30fc\u306b\u5927\u304d\u306a\u5909\u5316\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u3053\u306e\u7279\u9577\u304b\u3089\u3001\u73fe\u5728\u30de\u30fc\u30ab\u30fc\u89e3\u6790\u3092\u524d\u63d0\u3068\u3057\u305f\u5b9f\u9a13\u3067\u306fTrypLE\u3092\u4f7f\u3046\u3088\u3046\u306b\u3057\u3066\u3044\u307e\u3059\u300d\u3002\u7d30\u80de\u306e\u6027\u8cea\u3092\u640d\u306a\u308f\u306a\u3044\u3088\u3046\u30d7\u30ed\u30c8\u30b3\u30eb\u3092\u6d17\u7df4\u3057\u3001\u54c1\u8cea\u3092\u7cbe\u67fb\u3057\u305f\u5148\u306b\u306f\u300c\u3088\u308a\u52b9\u679c\u7684\u306a\u300d\u518d\u751f\u533b\u7642\u304c\u3042\u308b\u3068\u8a71\u3059\u8fbb\u6c0f\u3002\u4eca\u5f8c\u3001\u5897\u52a0\u3057\u3066\u3044\u304f\u3067\u3042\u308d\u3046\u7d30\u80de\u79fb\u690d\u6cbb\u7642\u306e\u8cea\u306e\u5411\u4e0a\u306bTrypLE\u306e\u69d8\u306a\u300c\u3088\u308a\u7d30\u80de\u969c\u5bb3\u6027\u306e\u5c11\u306a\u3044\u300d\u7d30\u80de\u5265\u96e2\u5264\u306e\u958b\u767a\u306f\u975e\u5e38\u306b\u91cd\u8981\u306a\u610f\u5473\u3092\u6301\u3064\u3068\u8003\u3048\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u81e8\u5e8a\u7814\u7a76\u306b\u9069\u3057\u305f\u7d30\u80de\u89e3\u96e2\u5264<br \/>\nCTS TrypLE Select Enzyme<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A1285901?cid=Social_LAB\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3793 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/P11_A1285901.jpg\" alt=\"CTS TrypLE Select Enzyme\" width=\"153\" height=\"250\" \/><\/a><\/p>\n<p>Gibco\u2122 CTS\u2122 TrypLE\u2122 Select Enzyme\u306f\u3001\u52d5\u7269\u6210\u5206\u7531\u6765\u6210\u5206\u3092\u542b\u307e\u306a\u3044\u7d30\u80de\u89e3\u96e2\u7528\u8a66\u85ac\u3067\u3042\u308a\u3001\u30c8\u30ea\u30d7\u30b7\u30f3\u4ee3\u66ff\u54c1\u3068\u3057\u3066\u4f7f\u3048\u307e\u3059\u3002\u3053\u306e\u88fd\u54c1\u3092\u542b\u3080Gibco\u2122 CTS\u2122 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Practice&#8221;\u3092\u610f\u5473\u3057\u3066\u304a\u308a\u3001\u672c\u90a6\u306b\u304a\u3051\u308b\u85ac\u6a5f\u6cd5\u4e0b\u306eGMP\u3068\u306f\u540c\u4e00\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002<\/span><\/p>\n<p>&nbsp;<\/p>\n<h2><span style=\"font-size: 12pt\">\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=Social_LAB\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-3659\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/foregroundimg.img_.full_.high_-3.png\" alt=\"NEXT2017\u5e747\u6708\u53f7\" width=\"167\" height=\"235\" 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rel=\"noopener\">NEXT\u306e\u30d0\u30c3\u30af\u30ca\u30f3\u30d0\u30fc\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u76ee\u7684\u304a\u3088\u3073\u305d\u306e\u624b\u7d9a\u304d\u4e0a\u3067\u306e\u4f7f\u7528\u306f\u3067\u304d\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>TrypLE\u306f\u3001\u6700\u3082\u7d30\u80de\u969c\u5bb3\u6027\u306e\u4f4e\u3044\u7d30\u80de\u5265\u96e2\u5264\u3067\u3042\u308b \u8fbb \u90a6\u548c \u6c0f \uff08\u6771\u4eac\u533b\u79d1\u6b6f\u79d1\u5927\u5b66\u5927\u5b66\u9662\u7dcf\u5408\u7814\u7a76\u79d1\u8edf\u9aa8\u518d\u751f\u5b66\u8b1b\u5ea7\u51c6\u6559\u6388\uff09 \u5c3e\u5cf6\u7f8e\u4ee3\u5b50 \u6c0f\uff08\u540c\u5927\u5b66\u904b\u52d5\u5668\u5916\u79d1\u5b66\uff09 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