{"id":13439,"date":"2023-03-09T18:15:12","date_gmt":"2023-03-09T09:15:12","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=13439"},"modified":"2025-01-21T15:16:36","modified_gmt":"2025-01-21T06:16:36","slug":"western-blot-protein-ts-4","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/western-blot-protein-ts-4\/","title":{"rendered":"\u8ee2\u5199\u6761\u4ef6\u306e\u9078\u3073\u65b9\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c4\u56de"},"content":{"rendered":"

\"\"
\n\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u5411\u3051\u306e\u88fd\u54c1\u9078\u3073\u306e\u30dd\u30a4\u30f3\u30c8\u30014\u56de\u76ee\u306f\u8ee2\u5199\u306b\u3064\u3044\u3066\u3067\u3059\u3002<\/p>\n

SDS-PAGE\u304c\u7d42\u308f\u3063\u305f\u5f8c\u306f\u3001\u30b2\u30eb\u5185\u3067\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5206\u96e2\u3057\u305f\u72b6\u614b\u3067\u3059\u3002\u3053\u306e\u307e\u307e\u6297\u4f53\u53cd\u5fdc\u304c\u3067\u304d\u308c\u3070\u3088\u3044\u306e\u3067\u3059\u304c\u3001\u30b2\u30eb\u5185\u3067\u306f\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u62e1\u6563\u3057\u307e\u3059\u3057\u3001\u6297\u4f53\u304c\u30b2\u30eb\u306e\u4e2d\u306b\u5165\u308b\u306e\u306b\u6642\u9593\u3092\u8981\u3057\u307e\u3059\u3057\u3001\u6271\u3044\u3065\u3089\u3044\u3067\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u5225\u306e\u652f\u6301\u4f53\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u79fb\u3059\u4f5c\u696d\u3092\u884c\u3044\u307e\u3057\u3087\u3046\u3002\u3064\u307e\u308a\u3001\u30b2\u30eb\u304b\u3089\u30e1\u30f3\u30d6\u30ec\u30f3\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u79fb\u52d5\u3055\u305b\u308b\u3001\u8ee2\u5199\u306e\u30b9\u30c6\u30c3\u30d7\u3067\u3059\u3002<\/p>\n

\u8ee2\u5199\u65b9\u6cd5\u306f\u3001Towbin\u3089\u304c1979\u5e74\u306b\u30bf\u30f3\u30af\u5f0f\u3068\u547c\u3070\u308c\u308b\u88c5\u7f6e\u3092\u6587\u732e\u3067\u767a\u8868\u3057\u3066\u4ee5\u6765\u3001\u8907\u6570\u306e\u65b9\u6cd5\u304c\u958b\u767a\u3055\u308c\u3001\u8a66\u3055\u308c\u3066\u304d\u307e\u3057\u305f\u3002\u8ee2\u5199\u65b9\u6cd5\u3060\u3051\u3067\u306a\u304f\u3001\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc\u3084\u30e1\u30f3\u30d6\u30ec\u30f3\u306a\u3069\u3001\u9078\u629e\u80a2\u306f\u591a\u7a2e\u591a\u69d8\u306b\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u307e\u305a\u306f\u3001\u8ee2\u5199\u65b9\u6cd5\u306e\u9078\u3073\u65b9\u304b\u3089\u78ba\u8a8d\u3057\u3066\u3044\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u8ee2\u5199\u65b9\u6cd5\u306e\u9078\u3073\u65b9<\/h2>\n

\"\"<\/p>\n

\u73fe\u5728\u3001\u3088\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u8ee2\u5199\u65b9\u6cd5\u3068\u3057\u3066\u3001\u30bf\u30f3\u30af\u5f0f\u3001\u30bb\u30df\u30c9\u30e9\u30a4\u5f0f\u3001\u30c9\u30e9\u30a4\u5f0f\u304c\u3042\u308a\u307e\u3059\u3002\u88681\u306b\u3001\u3053\u308c\u3089\u306e3\u3064\u306e\u8ee2\u5199\u65b9\u6cd5\u306e\u30e1\u30ea\u30c3\u30c8\u3084\u30c7\u30e1\u30ea\u30c3\u30c8\u306a\u3069\u3092\u793a\u3057\u307e\u3057\u305f\u3002<\/p>\n

\u88681\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3000\u8ee2\u5199\u65b9\u6cd5\u3068\u88c5\u7f6e\u306e\u7a2e\u985e<\/p>\n\n\n\n\n\n\n
<\/td>\n\u8ee2\u5199\u65b9\u6cd5<\/td>\n\u8ee2\u5199\u6642\u9593
\n\uff0f\u30d0\u30c3\u30d5\u30a1\u30fc\u91cf<\/td>\n		\u30e1\u30ea\u30c3\u30c8<\/td>\n\u30c7\u30e1\u30ea\u30c3\u30c8<\/td>\n\u88c5\u7f6e<\/td>\n<\/tr>\n
\u30bf\u30f3\u30af\u5f0f\u203b<\/sup>1<\/sup><\/td>\n\"\"<\/td>\n60\u5206\u7a0b\u5ea6
\n\uff0f300 mL\uff5e1 L\u7a0b\u5ea6
\n\uff08\u88c5\u7f6e\u306b\u3088\u308b\uff09<\/td>\n		\u30fb\u8ee2\u5199\u306e\u30e0\u30e9\u304c\u6bd4\u8f03\u7684\u8d77\u3053\u308a\u3065\u3089\u3044
\n\u30fb\u9ad8\u5206\u5b50\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u3082\u8ee2\u5199\u52b9\u7387\u304c\u9ad8\u304f\u306a\u308a\u3084\u3059\u3044
\n\u30fb\u51b7\u5374\u304c\u3067\u304d\u308b<\/td>\n		\u30fb\u30d0\u30c3\u30d5\u30a1\u30fc\u91cf\u304c\u591a\u304f\u5fc5\u8981\u3067\u3001\u6e96\u5099\u306b\u624b\u9593\u304c\u304b\u304b\u308b
\n\u30fb\u9ad8\u3044\u96fb\u6d41\u5024\u3068\u9577\u3044\u6642\u9593\u304c\u5fc5\u8981<\/td>\n		Invitrogen\u2122 Mini Blot Module<\/a>\u203b<\/sup>2<\/sup>\u3084Invitrogen\u2122 XCell II\u2122 Blot Module<\/a>\u203b<\/sup>2<\/sup><\/td>\n<\/tr>\n
\u30bb\u30df\u30c9\u30e9\u30a4\u5f0f<\/td>\n\"\"<\/td>\n30\u5206\uff5e60\u5206
\n\uff0f100 mL\u7a0b\u5ea6
\n\uff08\u88c5\u7f6e\u306b\u3088\u308b\uff09<\/td>\n		\u30fb\u30d0\u30c3\u30d5\u30a1\u30fc\u91cf\u304c\u5c11\u306a\u304f\u3066\u6e08\u3080<\/td>\n\u30fb\u9ad8\u5206\u5b50\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u306f\u3001\u30bf\u30f3\u30af\u5f0f\u3088\u308a\u8ee2\u5199\u52b9\u7387\u304c\u4f4e\u3044\u3053\u3068\u304c\u3042\u308b
\n\u30fb\u51b7\u5374\u304c\u3067\u304d\u306a\u3044<\/td>\n		Invitrogen\u2122 Power Blotter System<\/a><\/td>\n<\/tr>\n
\u30c9\u30e9\u30a4\u5f0f<\/td>\n\"\"<\/td>\n7\u5206\u7a0b\u5ea6
\n\uff08\u88c5\u7f6e\u306b\u3088\u308b\uff09<\/td>\n		\u30fb\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u4e0d\u8981\u3067\u3001\u6e96\u5099\u304c\u7c21\u5358
\n\u30fb\u77ed\u6642\u9593\u3067\u8ee2\u5199\u304c\u3067\u304d\u308b
\n\u30fb\u8ab0\u3067\u3082\u540c\u3058\u7d50\u679c\u304c\u5f97\u3089\u308c\u3084\u3059\u3044<\/td>\n		\u30fb\u9ad8\u5206\u5b50\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u306f\u3001\u30bf\u30f3\u30af\u5f0f\u3088\u308a\u8ee2\u5199\u52b9\u7387\u304c\u4f4e\u3044\u3053\u3068\u304c\u3042\u308b<\/td>\n\n

Invitrogen\u2122 iBlot2\u2122 System<\/a><\/p>\n

Invitrogen\u2122 Power Blotter System<\/a>\u203b<\/sup>3<\/sup><\/p>\n<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b1\uff1a\u30bf\u30f3\u30af\u5f0f\u306f\u3001\u30e1\u30fc\u30ab\u30fc\u3084\u4f01\u696d\u306b\u3088\u3063\u3066\u30a6\u30a7\u30c3\u30c8\u5f0f\uff08\u30e1\u30f3\u30d6\u30ec\u30f3\u3084\u30b2\u30eb\u3092\u30bf\u30f3\u30af\u5185\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6c88\u3081\u308b\u8ee2\u5199\u65b9\u6cd5\uff09\u3092\u6307\u3059\u5834\u5408\u3082\u3042\u308c\u3070\u3001\u30bb\u30df\u30a6\u30a7\u30c3\u30c8\u5f0f\uff08\u30e2\u30b8\u30e5\u30fc\u30eb\u3092\u4f7f\u7528\u3057\u3066\u3001\u30e2\u30b8\u30e5\u30fc\u30eb\u5185\u306b\u30e1\u30f3\u30d6\u30ec\u30f3\u3084\u30b2\u30eb\u3092\u30bb\u30c3\u30c8\u3059\u308b\u8ee2\u5199\u65b9\u6cd5\uff09\u3092\u6307\u3059\u5834\u5408\u3082\u3042\u308a\u307e\u3059\u3002\u3053\u306e\u8868\u3067\u306f\u3001\u3088\u308a\u591a\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u308b\u30bb\u30df\u30a6\u30a7\u30c3\u30c8\u5f0f\u3092\u7d39\u4ecb\u3057\u3066\u3044\u307e\u3059\u3002
\n\u203b2\uff1aMini Blot Module\u3092\u7528\u3044\u305f\u8ee2\u5199\u306b\u306fInvitrogen\u2122 Mini Gel Tank<\/a>\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u30bf\u30f3\u30af\u3001XCell II Blot Module\u3092\u7528\u3044\u305f\u8ee2\u5199\u306b\u306fInvitrogen\u2122 XCell SureLock\u2122 Mini-Cell<\/a>\u306e\u30d0\u30c3\u30d5\u30a1\u30fc\u30bf\u30f3\u30af\u304c\u5225\u9014\u5fc5\u8981\u3067\u3059\u3002\u6cf3\u52d5\u6642\u306b\u3082\u540c\u3058\u30d0\u30c3\u30d5\u30a1\u30fc\u30bf\u30f3\u30af\u3092\u4f7f\u7528\u3067\u304d\u308b\u305f\u3081\u3001\u3053\u308c\u3089\u306e\u88c5\u7f6e\u3092\u6cf3\u52d5\u88c5\u7f6e\u304b\u3064\u8ee2\u5199\u88c5\u7f6e\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u3068\u4fbf\u5229\u3067\u3059\u3002
\n\u203b3\uff1aPower Blotter System\u306f\u3001\u5c02\u7528\u306e\u30b9\u30bf\u30c3\u30af\u4f7f\u7528\u6642\u306e\u307f\u3001\u30c9\u30e9\u30a4\u5f0f\u3068\u3057\u3066\u8ee2\u5199\u304c\u53ef\u80fd\u3067\u3059\u3002<\/small><\/small><\/p>\n

\u88681\u306b\u793a\u3057\u305f3\u3064\u306e\u65b9\u6cd5\u306e\u3046\u3061\u3001\u7279\u306b\u9ad8\u5206\u5b50\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u51b7\u5374\u304c\u3067\u304d\u3066\u6642\u9593\u306e\u5ef6\u9577\u304c\u53ef\u80fd\u306a\u30bf\u30f3\u30af\u5f0f\u304c\u3001\u6700\u3082\u8ee2\u5199\u52b9\u7387\u304c\u9ad8\u304f\u306a\u308a\u3084\u3059\u3044\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u30bf\u30f3\u30af\u5f0f\u306f\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u591a\u304f\u4f7f\u7528\u3057\u3001\u6e96\u5099\u306b\u624b\u9593\u3082\u304b\u304b\u308a\u307e\u3059\u3057\u3001\u9ad8\u3044\u96fb\u6d41\u5024\u304c\u5fc5\u8981\u3067\u3059\u3002\u3053\u308c\u3089\u306e\u30c7\u30e1\u30ea\u30c3\u30c8\u3092\u30ab\u30d0\u30fc\u3057\u305f\u306e\u304cPower Blotter System<\/a>\u306a\u3069\u306e\u30bb\u30df\u30c9\u30e9\u30a4\u5f0f\u8ee2\u5199\u88c5\u7f6e\u3067\u3059\u3002\u30bf\u30f3\u30af\u5f0f\u307b\u3069\u30d0\u30c3\u30d5\u30a1\u30fc\u91cf\u3092\u5fc5\u8981\u3068\u305b\u305a\u3001\u307e\u305f\u3001\u30b2\u30eb\u306e\u9762\u4ee5\u5916\u306b\u306f\u96fb\u6d41\u304c\u6d41\u308c\u305a\u96fb\u6975\u9593\u306e\u8ddd\u96e2\u304c\u77ed\u3044\u305f\u3081\u3001\u767a\u71b1\u91cf\u3082\u6291\u3048\u3084\u3059\u3044\u3067\u3059\u3002\u3057\u304b\u3057\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u7528\u610f\u3059\u308b\u624b\u9593\u306f\u5909\u308f\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002<\/p>\n

\u3053\u306e\u30bb\u30df\u30c9\u30e9\u30a4\u5f0f\u304b\u3089\u3055\u3089\u306b\u9032\u5316\u3057\u305f\u306e\u304ciBlot 2 System<\/a>\u306a\u3069\u306e\u30c9\u30e9\u30a4\u5f0f\u3067\u3059\u3002\u30c9\u30e9\u30a4\u5f0f\u306f\u3001\u30e1\u30fc\u30ab\u30fc\u306b\u3088\u3063\u3066\u306f\u300c\u9ad8\u901f\u30bb\u30df\u30c9\u30e9\u30a4\u5f0f\u300d\u3068\u3082\u547c\u3070\u308c\u307e\u3059\u3002\u5c02\u7528\u306e\u56fa\u5f62\u5316\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001\u8ee2\u5199\u6642\u306e\u71b1\u3067\u6eb6\u89e3\u3057\u3066\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u5f79\u5272\u3092\u679c\u305f\u3059\u306e\u3067\u3001\u30d0\u30c3\u30d5\u30a1\u30fc\u3092\u7528\u610f\u3059\u308b\u5fc5\u8981\u304c\u306a\u304f\u3001\u304b\u3064\u77ed\u6642\u9593\u3067\u8ee2\u5199\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\u3053\u306e\u3088\u3046\u306b\u3001\u8ee2\u5199\u65b9\u6cd5\u306b\u306f\u305d\u308c\u305e\u308c\u4e00\u9577\u4e00\u77ed\u304c\u3042\u308a\u307e\u3059\u3002\u8ee2\u5199\u52b9\u7387\u3092\u512a\u5148\u3055\u305b\u3066\u30bf\u30f3\u30af\u5f0f\u306b\u3059\u308b\u5834\u5408\u3082\u3042\u308b\u3057\u3001\u6642\u9593\u77ed\u7e2e\u3092\u512a\u5148\u3055\u305b\u3066\u30c9\u30e9\u30a4\u5f0f\u3092\u9078\u3076\u5834\u5408\u3082\u3042\u308b\u3067\u3057\u3087\u3046\u3002\u7814\u7a76\u306e\u512a\u5148\u9806\u4f4d\u306b\u6700\u3082\u5408\u3063\u305f\u65b9\u6cd5\u3092\u9078\u3076\u3053\u3068\u3092\u304a\u52e7\u3081\u3057\u307e\u3059\u3002<\/p>\n

\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc\u306e\u9078\u3073\u65b9<\/h2>\n

\"\"
\n\u30bf\u30f3\u30af\u5f0f\u3068\u30bb\u30df\u30c9\u30e9\u30a4\u5f0f\u3067\u8ee2\u5199\u3059\u308b\u5834\u5408\u3001\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc\u304c\u5fc5\u9808\u3067\u3059\u3002\u30c9\u30e9\u30a4\u5f0f\u3067\u3082\u3001\u88c5\u7f6e\u3084\u6761\u4ef6\u306b\u3088\u3063\u3066\u306f\u3001\u8ee2\u5199\u524d\u306b\u96fb\u6c17\u6cf3\u52d5\u7d42\u4e86\u5f8c\u306e\u30b2\u30eb\u3092\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc\u306b\u6d78\u3059\u3053\u3068\u3067\u3001\u8ee2\u5199\u52b9\u7387\u304c\u5411\u4e0a\u3057\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002
\n\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc\u306f\u3001SDS-PAGE\u30b2\u30eb\u306b\u9069\u7528\u3059\u308b\u3082\u306e\u3092\u88682\u304b\u3089\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u88682\uff1a\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc<\/p>\n\n\n\n\n\n\n
\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n\u7d44\u6210<\/td>\n\u9069\u7528\u30b2\u30eb<\/td>\n<\/tr>\n
Invitrogen\u2122 NuPAGE\u2122 Transfer Buffer<\/a>
\n\uff0b10\uff5e20%\u30e1\u30bf\u30ce\u30fc\u30eb<\/td>\n		25 mM Bicine\u300125 mM Bis-Tris (free base)\u30011 mM EDTA pH 7.2\u300110\uff5e20%\u30e1\u30bf\u30ce\u30fc\u30eb\u203b<\/sup>1<\/sup><\/td>\nBis-Tris\u30b2\u30eb
\nTris-Acetate\u30b2\u30eb<\/td>\n<\/tr>\n
Invitrogen\u2122 Novex\u2122 Tris-Glycine Transfer Buffer<\/a>
\n\uff0b10\uff5e20%\u30e1\u30bf\u30ce\u30fc\u30eb<\/td>\n		12 mM Tris Base\u300196 mM Glycine pH 8.3\u300110\uff5e20%\u30e1\u30bf\u30ce\u30fc\u30eb\u203b<\/sup>1<\/sup><\/td>\nTris-Glycine\u30b2\u30eb
\nTris-Tricine\u30b2\u30eb<\/td>\n<\/tr>\n
Towbin\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc<\/td>\n25 mM Tris\u3001192 mM Glycine\u300110% \u30e1\u30bf\u30ce\u30fc\u30eb\u203b<\/sup>1<\/sup><\/td>\nTris-Glycine\u30b2\u30eb<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b1\uff1a\u30e1\u30bf\u30ce\u30fc\u30eb\u306f\u3001\u30a8\u30bf\u30ce\u30fc\u30eb\u3067\u4ee3\u7528\u3067\u304d\u307e\u3059\u3002<\/small><\/small><\/p>\n

\uff1c\u30e1\u30bf\u30ce\u30fc\u30eb\u6fc3\u5ea6\u3068SDS\u6fc3\u5ea6\u306b\u3064\u3044\u3066\uff1e<\/b>
\n\u30e1\u30bf\u30ce\u30fc\u30eb\u306f\u30e1\u30f3\u30d6\u30ec\u30f3\u3078\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u7d50\u5408\u529b\u3092\u9ad8\u3081\u308b\u305f\u3081\u3001\u6dfb\u52a0\u306b\u3088\u308a\u8ee2\u5199\u52b9\u7387\u3092\u4e0a\u3052\u3089\u308c\u308b\u53ef\u80fd\u6027\u304c\u3042\u308a\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u30b2\u30eb\u3092\u30b7\u30e5\u30ea\u30f3\u30af\u3055\u305b\u308b\u52b9\u679c\u3082\u3042\u308a\u307e\u3059\u306e\u3067\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u6fc3\u5ea6\u304c\u9ad8\u3044\u3068\u9006\u306b\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30b2\u30eb\u304b\u3089\u51fa\u3065\u3089\u304f\u306a\u308a\u3001\u8ee2\u5199\u52b9\u7387\u304c\u4e0b\u304c\u308b\u3053\u3068\u3082\u3002\u307e\u305f\u30011\u30e2\u30b8\u30e5\u30fc\u30eb\u5185\uff08\u540c\u3058\u30b5\u30f3\u30c9\u30a4\u30c3\u30c1\u5185\uff09\u30672\u679a\u540c\u6642\u306b\u8ee2\u5199\u3059\u308b\u3068\u304d\u306f\u30011\u679a\u76ee\u306e\u30b2\u30eb\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c2\u679a\u76ee\u306e\u30e1\u30f3\u30d6\u30ec\u30f3\u307e\u3067\u8ee2\u5199\u3055\u308c\u3066\u3057\u307e\u308f\u306a\u3044\u3088\u3046\u306b\u300120%\u304c\u63a8\u5968\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u88682\u3067\u306f\u30d5\u30a1\u30fc\u30b9\u30c8\u30c1\u30e7\u30a4\u30b9\u306e\u30e1\u30bf\u30ce\u30fc\u30eb\u6fc3\u5ea6\u3092\u63b2\u8f09\u3057\u3066\u3044\u307e\u3059\u304c\u3001\u8ee2\u5199\u52b9\u7387\u304c\u4f4e\u3044\u3068\u304d\u306f\u3001\u30e1\u30bf\u30ce\u30fc\u30eb\u6fc3\u5ea6\u3092\u5909\u66f4\u3059\u308b\u3068\u6539\u5584\u3059\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u307e\u305f\u3001\u88682\u306b\u8a18\u8f09\u306e\u8ee2\u5199\u30d0\u30c3\u30d5\u30a1\u30fc\u306bSDS\u3092\u52a0\u3048\u308b\u3053\u3068\u3067\u3082\u3001\u8ee2\u5199\u52b9\u7387\u3092\u6539\u5584\u3067\u304d\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u8ee2\u5199\u52b9\u7387\u304c\u4f4e\u3044\u3068\u304d\u306f\u3001\u307e\u305a\u7d42\u6fc3\u5ea60.01%\uff5e0.05%\u3067\u8a66\u3057\u3066\u307f\u308b\u306e\u304c\u304a\u52e7\u3081\u3067\u3059\u3002SDS\u306f\u3001\u30b2\u30eb\u5185\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u3088\u308a\u5747\u4e00\u306b\u30de\u30a4\u30ca\u30b9\u96fb\u8377\u306b\u3057\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u6eb6\u51fa\u3055\u305b\u3084\u3059\u304f\u3057\u307e\u3059\u3002\u4e00\u65b9\u3067\u3001\u30e1\u30f3\u30d6\u30ec\u30f3\u3078\u306e\u7d50\u5408\u52b9\u7387\u3092\u4f4e\u4e0b\u3055\u305b\u3001\u30a4\u30aa\u30f3\u5f37\u5ea6\u304c\u9ad8\u304f\u306a\u308b\u3053\u3068\u3067\u71b1\u3082\u767a\u751f\u3057\u3084\u3059\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n

\u30e1\u30bf\u30ce\u30fc\u30eb\u3082SDS\u3082\u3001\u300c\u4f55%\u304c\u30d9\u30b9\u30c8\u300d\u3068\u306f\u8a00\u3048\u305a\u3001\u30b2\u30eb\u6fc3\u5ea6\u3084\u8ee2\u5199\u65b9\u6cd5\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u306e\u5206\u5b50\u91cf\u306a\u3069\u306b\u3088\u3063\u3066\u3082\u7570\u306a\u308a\u307e\u3059\u306e\u3067\u3001\u7d50\u679c\u3092\u898b\u306a\u304c\u3089\u6fc3\u5ea6\u3092\u691c\u8a0e\u3057\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u3053\u306e\u30d6\u30ed\u30b0\u3082\u53c2\u8003\u306b\u306a\u308a\u307e\u3059\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306b\u304a\u3051\u308b\u8ee2\u5199\u52b9\u7387\u306e\u6700\u9069\u5316<\/a><\/p>\n

\u30e1\u30f3\u30d6\u30ec\u30f3\u306e\u9078\u3073\u65b9<\/h2>\n

\"\"
\n\u6700\u5f8c\u306b\u3001\u691c\u51fa\u307e\u3067\u9577\u3044\u4ed8\u304d\u5408\u3044\u306b\u306a\u308b\u30e1\u30f3\u30d6\u30ec\u30f3\u306e\u9078\u3073\u65b9\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3057\u3087\u3046\u3002\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u4e3b\u306b\u4f7f\u308f\u308c\u308b\u306e\u306f\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u30e1\u30f3\u30d6\u30ec\u30f3\u3068PVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u3067\u3059\u3002<\/p>\n

\u88683\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u3067\u4f7f\u7528\u3055\u308c\u308b\u30e1\u30f3\u30d6\u30ec\u30f3<\/p>\n\n\n\n\n\n
\u30e1\u30f3\u30d6\u30ec\u30f3<\/td>\n\u30bf\u30f3\u30d1\u30af\u8cea\u7d50\u5408\u529b<\/td>\n\u30e1\u30ea\u30c3\u30c8<\/td>\n\u30c7\u30e1\u30ea\u30c3\u30c8<\/td>\n<\/tr>\n
PVDF<\/td>\n\u9ad8<\/td>\n\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u7d50\u5408\u529b\u304c\u9ad8\u3044
\n\u30fb\u7834\u308c\u306b\u304f\u3044<\/td>\n		\u30fb\u4f7f\u7528\u524d\u306b\u30e1\u30bf\u30ce\u30fc\u30eb\uff08\u30a8\u30bf\u30ce\u30fc\u30eb\uff09\u3067\u89aa\u6c34\u5316\u3059\u308b\u5fc5\u8981\u304c\u3042\u308b\u203b<\/sup>
\n\u30fb\u81ea\u5bb6\u86cd\u5149\u304c\u3042\u308b\u305f\u3081\u3001\u86cd\u5149\u691c\u51fa\u6642\u306f\u4f4e\u86cd\u5149PVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u304c\u63a8\u5968<\/td>\n<\/tr>\n
\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9<\/td>\n\u4e2d<\/td>\n\u30fb\u4f7f\u7528\u524d\u306e\u89aa\u6c34\u5316\u51e6\u7406\u304c\u4e0d\u8981
\n\u30fb\u5b89\u4fa1<\/td>\n		\u30fbPVDF\u3088\u308a\u3082\u30bf\u30f3\u30d1\u30af\u8cea\u7d50\u5408\u529b\u304c\u4f4e\u3044
\n\u30fb\u7269\u7406\u7684\u306a\u5f37\u5ea6\u304c\u4f4e\u304f\u3001\u30ea\u30d7\u30ed\u30fc\u30d3\u30f3\u30b0\u306b\u306f\u5411\u304b\u306a\u3044\u5834\u5408\u304c\u3042\u308b<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

\u203b\u9ad8\u901f\u30bb\u30df\u30c9\u30e9\u30a4\u5f0f\uff08\u30c9\u30e9\u30a4\u5f0f\uff09\u8ee2\u5199\u88c5\u7f6e\u7528\u306e\u30b9\u30bf\u30c3\u30af\u3067\u306f\u3001\u3042\u3089\u304b\u3058\u3081PVDF\u304c\u89aa\u6c34\u5316\u51e6\u7406\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u307e\u305f\u3001\u3042\u3089\u304b\u3058\u3081\u89aa\u6c34\u5316\u3055\u308c\u305fPVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u3082\u5e02\u8ca9\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u88c5\u7f6e\u3084\u88fd\u54c1\u306b\u3088\u308a\u307e\u3059\u306e\u3067\u3001\u4e8b\u524d\u306b\u78ba\u8a8d\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/small><\/small><\/p>\n

PVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u306f\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u3088\u308a\u3082\u30bf\u30f3\u30d1\u30af\u8cea\u7d50\u5408\u529b\u304c\u9ad8\u304f\u3001\u7269\u7406\u7684\u5f37\u5ea6\u3082\u9ad8\u304f\u3066\u4e08\u592b\u3067\u3059\u3002\u691c\u51fa\u5f8c\u306b\u30ea\u30d7\u30ed\u30fc\u30d3\u30f3\u30b0\u3092\u3057\u305f\u3044\u5834\u5408\u306fPVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u3092\u9078\u3076\u3068\u826f\u3044\u3067\u3057\u3087\u3046\u3002
\n\u4e00\u65b9\u3067\u3001PVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u306f\u4f7f\u7528\u524d\u306b\u30e1\u30bf\u30ce\u30fc\u30eb\u3084\u30a8\u30bf\u30ce\u30fc\u30eb\u3067\u306e\u89aa\u6c34\u5316\u304c\u5fc5\u8981\u3067\u3059\uff08\u3042\u3089\u304b\u3058\u3081\u89aa\u6c34\u5316\u3055\u308c\u305fPVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u3082\u5e02\u8ca9\u3055\u308c\u3066\u3044\u307e\u3059\uff09\u3002\u89aa\u6c34\u5316\u304c\u5fc5\u8981\u306aPVDF\u30e1\u30f3\u30d6\u30ec\u30f3\u3067\u306f\u3001\u53cd\u5fdc\u4e2d\u306b\u4e00\u5ea6\u4e7e\u3044\u3066\u3057\u307e\u3046\u3068\u518d\u5ea6\u89aa\u6c34\u5316\u304c\u5fc5\u8981\u306b\u306a\u308b\u305f\u3081\u3001\u3053\u308c\u3092\u907f\u3051\u305f\u3044\u5834\u5408\u306f\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u30e1\u30f3\u30d6\u30ec\u30f3\u3092\u9078\u3073\u307e\u3057\u3087\u3046\u3002\u305f\u3060\u3001\u30cb\u30c8\u30ed\u30bb\u30eb\u30ed\u30fc\u30b9\u30e1\u30f3\u30d6\u30ec\u30f3\u306f\u7269\u7406\u7684\u306a\u5f37\u5ea6\u304c\u4f4e\u3044\u305f\u3081\u3001\u30ea\u30d7\u30ed\u30fc\u30d3\u30f3\u30b0\u306b\u306f\u5411\u304b\u306a\u3044\u5834\u5408\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u307e\u305f\u3001\u30e1\u30f3\u30d6\u30ec\u30f3\u306e\u5b54\u5f84\u306f\u30010.45 \u03bcm\u30840.2 \u03bcm\u306a\u3069\u4f55\u7a2e\u985e\u304b\u8ca9\u58f2\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u4f4e\u5206\u5b50\u30bf\u30f3\u30d1\u30af\u8cea\u306f\u3001\u5b54\u5f84\u304c\u5927\u304d\u3044\u3068\u30e1\u30f3\u30d6\u30ec\u30f3\u3092\u901a\u308a\u629c\u3051\u3066\u3057\u307e\u3044\u307e\u3059\u3002\u76ee\u5b89\u3068\u3057\u306620 kDa\u4ee5\u4e0b\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30bf\u30fc\u30b2\u30c3\u30c8\u306e\u5834\u5408\u306f\u3001\u5b54\u5f840.2 \u03bcm\u306e\u30e1\u30f3\u30d6\u30ec\u30f3\u3092\u9078\u3073\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u304a\u307e\u3051\uff1a\u8ee2\u5199\u52b9\u7387\u306e\u78ba\u8a8d\u65b9\u6cd5<\/h2>\n

\u3068\u3053\u308d\u3067\u3001\u8ee2\u5199\u304c\u3046\u307e\u304f\u3044\u3063\u305f\u304b\u3069\u3046\u304b\u3001\u3069\u306e\u3088\u3046\u306b\u78ba\u8a8d\u3059\u308c\u3070\u3088\u3044\u306e\u3067\u3057\u3087\u3046\u304b\u3002
\n\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306f\u30b9\u30c6\u30c3\u30d7\u6570\u304c\u591a\u3044\u624b\u6cd5\u3067\u3059\u3002\u6297\u539f\u6297\u4f53\u53cd\u5fdc\u3092\u7d42\u3048\u3066\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u691c\u51fa\u3067\u304d\u306a\u304b\u3063\u305f\u3068\u304d\u3001\u8ee2\u5199\u52b9\u7387\u306e\u4f4e\u3055\u3092\u7591\u3046\u306e\u306f\u5c11\u3057\u65e9\u8a08\u3067\u3059\u3002\u306a\u305c\u306a\u3089\u3001\u691c\u51fa\u306b\u306f\u6297\u4f53\u53cd\u5fdc\u3084\u691c\u51fa\u8a66\u85ac\u3068\u306e\u53cd\u5fdc\u306a\u3069\u3001\u3055\u307e\u3056\u307e\u306a\u8981\u7d20\u304c\u7d61\u3080\u305f\u3081\u3001\u8ee2\u5199\u52b9\u7387\u304c\u4f4e\u304b\u3063\u305f\u3068\u306f\u9650\u3089\u306a\u3044\u305f\u3081\u3067\u3059\u3002<\/p>\n

\u8ee2\u5199\u306e\u78ba\u8a8d\u306b\u306f\u3001\u30e1\u30f3\u30d6\u30ec\u30f3\u4e0a\u306e\u7740\u8272\u6e08\u307f\u30de\u30fc\u30ab\u30fc\u306e\u76ee\u8996\u78ba\u8a8d<\/b>\u3001\u3053\u308c\u304c\u6700\u3082\u7c21\u4fbf\u306a\u65b9\u6cd5\u3067\u3059\u3002\u8ee2\u5199\u5f8c\u306f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u30bf\u30f3\u30d1\u30af\u8cea\u3068\u540c\u3058\u5206\u5b50\u91cf\u306e\u30de\u30fc\u30ab\u30fc\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30e1\u30f3\u30d6\u30ec\u30f3\u4e0a\u306b\u898b\u3048\u3066\u3044\u308b\u304b\u3001\u3057\u3063\u304b\u308a\u78ba\u8a8d\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n

\"\"<\/p>\n

\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u305f\u3081\u306e\u8ee2\u5199\u65b9\u6cd5\u306e\u9078\u3073\u65b9<\/h2>\n

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\u6b21\u56de<\/a>\u306f\u3001\u30e1\u30f3\u30d6\u30ec\u30f3\u4e0a\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u306a\u3044\u90e8\u5206\u3092\u5927\u91cf\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u3067\u8986\u3044\u307e\u3057\u3087\u3046\u3002\u3064\u307e\u308a\u3001\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u306e\u30dd\u30a4\u30f3\u30c8\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u30b7\u30ea\u30fc\u30ba\u306f\u3053\u3061\u3089\uff1a
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\n\u30d6\u30ed\u30c3\u30ad\u30f3\u30b0\u8a66\u85ac\u306e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u88fd\u54c1\u9078\u3073\u306b\u3082\u3046\u56f0\u3089\u306a\u3044\uff01\u7b2c5\u56de<\/a>
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\n\u5316\u5b66\u767a\u5149\u3068\u30de\u30eb\u30c1\u30d7\u30ec\u30c3\u30af\u30b9\u691c\u51fa\u306e\u9078\u629e\u30dd\u30a4\u30f3\u30c8\uff1a\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u9078\u3073\u65b9\u3000\u7b2c7\u56de<\/a><\/p>\n

\u3010\u7121\u6599\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3011\u30bf\u30f3\u30d1\u30af\u8cea\u89e3\u6790\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u30cf\u30f3\u30c9\u30d6\u30c3\u30af<\/h3>\n

\u52b9\u7387\u7684\u306a\u30bf\u30f3\u30d1\u30af\u8cea\u62bd\u51fa\u304b\u3089\u30a6\u30a7\u30b9\u30bf\u30f3\u30d6\u30ed\u30c3\u30c6\u30a3\u30f3\u30b0\u306e\u89e3\u6790\u30c4\u30fc\u30eb\u307e\u3067\u3001\u5305\u62ec\u7684\u306b\u30bd\u30ea\u30e5\u30fc\u30b7\u30e7\u30f3\u3092\u7d39\u4ecb\u3057\u3066\u304a\u308a\u307e\u3059\u3002PDF\u30d5\u30a1\u30a4\u30eb\u306e\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3092\u3054\u5e0c\u671b\u306e\u65b9\u306f\u3001\u4e0b\u8a18\u30dc\u30bf\u30f3\u3088\u308a\u304a\u7533\u8fbc\u307f\u304f\u3060\u3055\u3044\u3002<\/p>\n

\u30c0\u30a6\u30f3\u30ed\u30fc\u30c9\u3059\u308b<\/a><\/p>\n

 <\/p>\n

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