{"id":13660,"date":"2023-03-30T09:12:57","date_gmt":"2023-03-30T00:12:57","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=13660"},"modified":"2024-09-18T16:10:49","modified_gmt":"2024-09-18T07:10:49","slug":"ce-workshop-gsd-ts-1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/ce-workshop-gsd-ts-1\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306b\u6642\u9593\u304c\u7d4c\u904e\u3057\u3066\u3082\u5927\u4e08\u592b\uff1f"},"content":{"rendered":"<p>DNA\u306e\u5869\u57fa\u914d\u5217\u3092\u6c7a\u5b9a\u3059\u308b\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u6cd5\u306f\u3001\u4eca\u3067\u3082\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u78ba\u8a8d\u304b\u3089\u907a\u4f1d\u5b50\u8a3a\u65ad\u7814\u7a76\u307e\u3067\u5e45\u5e83\u304f\u4f7f\u7528\u3055\u308c\u3066\u3044\u308b\u65b9\u6cd5\u3067\u3059\u3002\u4eca\u56de\u306e\u30d6\u30ed\u30b0\u3067\u306fPCR\u88c5\u7f6e\u3067\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u304c\u7d42\u308f\u3063\u305f\u3089\u3001\u305d\u306e\u307e\u307ePCR\u88c5\u7f6e\u306b\u7f6e\u3044\u3066\u304a\u3044\u3066\u3082\u5927\u4e08\u592b\u3067\u3059\u304b\uff1f\u3069\u306e\u304f\u3089\u3044\u653e\u7f6e\u3057\u3066\u3082\u5927\u4e08\u592b\u3067\u3059\u304b\uff1f\u3068\u3044\u3046\u304a\u58f0\u3092\u53c2\u8003\u306b\u3001\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u7523\u7269\u3092\u4e00\u5b9a\u671f\u9593\u4fdd\u7ba1\u3057\u305f\u5f71\u97ff\u3092\u78ba\u8a8d\u3057\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<h2>\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n<p><b>\u4f7f\u7528\u8a66\u85ac<\/b><\/p>\n<ul>\n<li>pGEM-3Zf(+) DNA\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\u30d9\u30af\u30bf\u30fc(200ng\/uL)<\/li>\n<li>-21M13\u30d7\u30e9\u30a4\u30de\u30fc(0.8pmol\/uL)<\/li>\n<li>Applied Biosystems\u2122 BigDye\u2122 Terminator v3.1 Cycle Sequencing Kit<\/li>\n<li>Applied Biosystems\u2122 BigDye\u2122 XTerminator\u2122 Purification Kit<\/li>\n<\/ul>\n<p>\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u306f\u4ee5\u4e0b\u306e\u6761\u4ef6\u3067\u8abf\u88fd<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"275\">Ready Reaction Mix<br \/>\nx5 Sequencing Buffer<br \/>\n\u30b3\u30f3\u30c8\u30ed\u30fc\u30ebDNA\u30d9\u30af\u30bf\u30fc<br \/>\n\u30d7\u30e9\u30a4\u30de\u30fc<br \/>\n\u8131\u30a4\u30aa\u30f3\u6c34<\/td>\n<td width=\"275\">2 \u03bcL<br \/>\n3 \u03bcL<br \/>\n1 \u03bcL<br \/>\n4 \u03bcL<br \/>\n10 \u03bcL<\/td>\n<\/tr>\n<tr>\n<td width=\"275\">\u5168\u91cf<\/td>\n<td width=\"275\">20 \u03bcL<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p>Applied Biosystems\u2122 MiniAmp\u2122 Plus Thermal Cycler\u3092\u4f7f\u7528\u3057\u6a19\u6e96\u306e\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u3092\u5b9f\u65bd<\/p>\n<p>\u53cd\u5fdc\u5f8c\u306e\u7523\u7269\u306f\u4ee5\u4e0b\u306e3\u3064\u306e\u6761\u4ef6\u3092\u691c\u8a3c<\/p>\n<ol>\n<li>\u3059\u3050\u306b\u7cbe\u88fd\u30b9\u30c6\u30c3\u30d7\u3078<\/li>\n<li>\u51b7\u8535\u30fb\u906e\u5149\u30671\u65e5\u4fdd\u7ba1\u5f8c\u3001\u7cbe\u88fd\u30b9\u30c6\u30c3\u30d7\u3078<\/li>\n<li>\u51b7\u8535\u30fb\u906e\u5149\u30677\u65e5\u4fdd\u7ba1\u5f8c\u3001\u7cbe\u88fd\u30b9\u30c6\u30c3\u30d7\u3078<\/li>\n<\/ol>\n<p>BigDye XTerminator Purification Kit\u3067\u7cbe\u88fd<br \/>\nApplied Biosystems\u2122 SeqStudio\u2122 Genetic Analyzer\u3092\u4f7f\u7528\u3057LongSeq_BDX\u306e\u6761\u4ef6\u3067\u30e9\u30f3\u3092\u5b9f\u65bd<\/p>\n<h2>\u7d50\u679c<\/h2>\n<p>\u6570\u5024\u306f\u3059\u3079\u3066n=2\u3001\u540c\u4e00\u30b5\u30f3\u30d7\u30eb\u30922\u56de\u30e9\u30f3\u3057\u305f\u5e73\u5747\u5024\u3092\u7b97\u51fa\u3057\u3066\u3044\u307e\u3059\u3002<\/p>\n<ul>\n<li>\u9ad8\u3044\u30af\u30aa\u30ea\u30c6\u30a3\uff08QV\u502420\u4ee5\u4e0a\uff09\u3067\u5869\u57fa\u914d\u5217\u6c7a\u5b9a\u3055\u308c\u305f\u9023\u7d9a\u5869\u57fa\u9577<\/li>\n<\/ul>\n<p style=\"padding-left: 40px\">\u6570\u5024\u306fSequencing Analysis Software Report\u306eLength of Read\u3092\u4f7f\u7528<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"196\">\u3059\u3050\u306b\u7cbe\u88fd\u30b9\u30c6\u30c3\u30d7<\/td>\n<td width=\"129\"><strong>878 bp<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"196\">\u51b7\u8535\u30fb\u906e\u5149\u30671\u65e5\u4fdd\u7ba1<\/td>\n<td width=\"129\"><strong>850 bp<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"196\">\u51b7\u8535\u30fb\u906e\u5149\u30677\u65e5\u4fdd\u7ba1<\/td>\n<td width=\"129\"><strong>882 bp<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"padding-left: 40px\">\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306b7\u65e5\u9593\u4fdd\u7ba1\u3057\u3066\u3082\u9069\u5207\u306b\u5869\u57fa\u914d\u5217\u6c7a\u5b9a\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<ul>\n<li>\u30b5\u30f3\u30d7\u30eb\u30d4\u30fc\u30af\u306e\u9ad8\u3055\u306e\u5e73\u5747\u5024<\/li>\n<\/ul>\n<p style=\"padding-left: 40px\">\u6570\u5024\u306fSequencing Analysis Software Annotation\u306eAve Signal Intensity\u306e\u5e73\u5747\u5024\u3092\u4f7f\u7528<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"196\">\u3059\u3050\u306b\u7cbe\u88fd\u30b9\u30c6\u30c3\u30d7<\/td>\n<td width=\"129\"><strong>718<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"196\">\u51b7\u8535\u30fb\u906e\u5149\u30671\u65e5\u4fdd\u7ba1<\/td>\n<td width=\"129\"><strong>565<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"196\">\u51b7\u8535\u30fb\u906e\u5149\u30677\u65e5\u4fdd\u7ba1<\/td>\n<td width=\"129\"><strong>496<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"padding-left: 40px\">\u30b5\u30f3\u30d7\u30eb\u30d4\u30fc\u30af\u306e\u9ad8\u3055\u306f1\u65e5\u4fdd\u7ba1\u3067\u7d0420\uff05\u30017\u65e5\u4fdd\u7ba1\u3067\u7d0430\uff05\u4f4e\u4e0b\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u4e0b\u56f3\u306fSequencing Analysis Software\u306eRaw\u30c7\u30fc\u30bf\u3000\uff39\u8ef8\u306f3000\u306b\u30bb\u30c3\u30c8<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-13692\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/image-tsma2301-1.jpg\" alt=\"\" width=\"1363\" height=\"284\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/image-tsma2301-1.jpg 1363w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/image-tsma2301-1-300x63.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/image-tsma2301-1-1024x213.jpg 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/image-tsma2301-1-768x160.jpg 768w\" sizes=\"auto, (max-width: 1363px) 100vw, 1363px\" \/><\/p>\n<ul>\n<li>\u30b5\u30f3\u30d7\u30eb\u30d4\u30fc\u30af\u306e\u30b7\u30b0\u30ca\u30eb\/\u30ce\u30a4\u30ba<\/li>\n<\/ul>\n<p style=\"padding-left: 40px\">\u6570\u5024\u306fSequencing Analysis Software Report\u306eAvg S\/N\u3092\u4f7f\u7528<\/p>\n<table>\n<tbody>\n<tr>\n<td width=\"196\">\u3059\u3050\u306b\u7cbe\u88fd\u30b9\u30c6\u30c3\u30d7<\/td>\n<td width=\"129\"><strong>152<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"196\">\u51b7\u8535\u30fb\u906e\u5149\u30671\u65e5\u4fdd\u7ba1<\/td>\n<td width=\"129\"><strong>128<\/strong><\/td>\n<\/tr>\n<tr>\n<td width=\"196\">\u51b7\u8535\u30fb\u906e\u5149\u30677\u65e5\u4fdd\u7ba1<\/td>\n<td width=\"129\"><strong>114<\/strong><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p style=\"padding-left: 40px\">\u30b5\u30f3\u30d7\u30eb\u30d4\u30fc\u30af\u306e\u30b7\u30b0\u30ca\u30eb\/\u30ce\u30a4\u30ba\u306f1\u65e5\u4fdd\u7ba1\u3067\u7d0415 \uff05\u30017\u65e5\u4fdd\u7ba1\u306725 \uff05\u4f4e\u4e0b\u3057\u307e\u3057\u305f\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u3044\u304b\u304c\u3067\u3057\u305f\u3067\u3057\u3087\u3046\u304b\u3002<br \/>\n\u53cd\u5fdc\u5f8c\u306f\u65e9\u3081\u306b\u6b21\u306e\u7cbe\u88fd\u30b9\u30c6\u30c3\u30d7\u306b\u3059\u3059\u3093\u3060\u65b9\u304c\u3088\u3044\u3088\u3046\u3067\u3059\u3002<\/p>\n<p>\u4eca\u56de\u306e\u8a66\u9a13\u3067\u306f\u3001\u5869\u57fa\u914d\u5217\u6c7a\u5b9a\u3055\u308c\u305f\u9577\u3055\u306b\u5f71\u97ff\u306f\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001\u30b5\u30a4\u30af\u30eb\u30b7\u30fc\u30b1\u30f3\u30b9\u53cd\u5fdc\u5f8c\u306e\u4fdd\u7ba1\u671f\u9593\u306e\u9577\u3055\u306b\u3088\u308a\u3001\u30b5\u30f3\u30d7\u30eb\u30d4\u30fc\u30af\u3068\u30b7\u30b0\u30ca\u30eb\/\u30ce\u30a4\u30ba\u306e\u4f4e\u4e0b\u304c\u307f\u3089\u308c\u307e\u3057\u305f\u3002<br \/>\n\u30b5\u30f3\u30d7\u30eb\u30d4\u30fc\u30af\u306e\u9ad8\u3055\u306f\u3001\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u306e\u8cea\u3084\u91cf\u3001\u914d\u5217\u60c5\u5831\u306b\u4f9d\u5b58\u3057\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u4fdd\u7ba1\u671f\u9593\u306e\u9577\u3055\u3060\u3051\u3067\u306a\u304f\u30c6\u30f3\u30d7\u30ec\u30fc\u30c8DNA\u3084\u53cd\u5fdc\u6761\u4ef6\u306b\u3088\u3063\u3066\u306f\u3001\u30b5\u30f3\u30d7\u30eb\u30d4\u30fc\u30af\u306e\u4f4e\u4e0b\u304c\u5869\u57fa\u6c7a\u5b9a\u306b\u5f71\u97ff\u3092\u4e0e\u3048\u308b\u53ef\u80fd\u6027\u3082\u8003\u3048\u3089\u308c\u307e\u3059\u3002<\/p>\n<h3>\u3010\u7121\u6599\u516c\u958b\u4e2d\u3011\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u57fa\u790e<\/h3>\n<p>\u3053\u306e\u30b1\u30df\u30b9\u30c8\u30ea\u30fc\u30ac\u30a4\u30c9\u3067\u306f\u3001\u30ef\u30fc\u30af\u30d5\u30ed\u30fc\u304b\u3089\u30a2\u30d7\u30ea\u30b1\u30fc\u30b7\u30e7\u30f3\u307e\u3067\u30b5\u30f3\u30ac\u30fc\u30b7\u30fc\u30b1\u30f3\u30b9\u306e\u57fa\u672c\u539f\u7406\u3092\u5b66\u3079\u307e\u3059\u3002<\/p>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/global\/forms\/sanger-sequencing-guide-download.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_ce_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener 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