{"id":13708,"date":"2023-04-10T11:17:03","date_gmt":"2023-04-10T02:17:03","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=13708"},"modified":"2023-12-27T17:27:49","modified_gmt":"2023-12-27T08:27:49","slug":"basic-workshop-gsd-ts-1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/basic-workshop-gsd-ts-1\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011\u5bd2\u5929\u3067DNA\u3092\u96fb\u6c17\u6cf3\u52d5\uff1fAgarose\u3067\u306f\u306a\u304fAgar\u3092\u4f7f\u3063\u3066\u307f\u305f"},"content":{"rendered":"<p>\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306f\u3001DNA\u306a\u3069\u306e\u6838\u9178\u3092\u691c\u51fa\u3059\u308b\u4e00\u822c\u7684\u306a\u65b9\u6cd5\u3067\u3059\u3002\u305d\u306e\u6b74\u53f2\u306f1960\u5e74\u4ee3\u5f8c\u534a\u306b\u307e\u3067\u3055\u304b\u306e\u307c\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/cloning-learning-center\/invitrogen-school-of-molecular-biology\/na-electrophoresis-education\/na-separation-overview.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_basic_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u6838\u9178\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306e\u6b74\u53f2<\/a>\uff09\u30021960\u5e74\u4ee3\u521d\u982d\u306e\u96fb\u6c17\u6cf3\u52d5\u3067\u306f\u6eb6\u6db2\u4e2d\u3067DNA\u306e\u79fb\u52d5\u5ea6\u3092\u8abf\u3079\u3066\u3044\u305f\u3088\u3046\u3067\u3059\u3002\u305d\u306e\u5f8c\u3001\u6570\u5e74\u306e\u3046\u3061\u306b\u30dd\u30ea\u30a2\u30af\u30ea\u30eb\u30a2\u30df\u30c9\u30fb\u5bd2\u5929\uff08Agar\uff09\u30fb\u30a2\u30ac\u30ed\u30fc\u30b9\uff08Agarose\uff09\u306a\u3069\u306e\u30b2\u30eb\u30de\u30c8\u30ea\u30c3\u30af\u30b9\u304c\u6709\u52b9\u3067\u3042\u308b\u3053\u3068\u304c\u898b\u3044\u3060\u3055\u308c\u307e\u3057\u305f\u3002<\/p>\n<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>\u73fe\u5728\u3067\u306f\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u3092\u4f7f\u7528\u3057\u3066\u96fb\u6c17\u6cf3\u52d5\u3057DNA\u3092\u30b5\u30a4\u30ba\u3054\u3068\u306b\u5206\u96e2\u3059\u308b\u3053\u3068\u304c\u4e00\u822c\u7684\u3067\u3059\u3002\u30a2\u30eb\u30d5\u30a1\u30d9\u30c3\u30c8\u9806\u306b\u8a66\u85ac\u3092\u6574\u7406\u3057\u3066\u3044\u308b\u30e9\u30dc\u3067\u306f\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\uff08Agarose\uff09\u306e\u8fd1\u304f\u306b\u5bd2\u5929\uff08Agar\uff09\u304c\u3042\u308b\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u30a2\u30ac\u30ed\u30fc\u30b9\u306f\u96fb\u6c17\u6cf3\u52d5\u306b\u3001\u5bd2\u5929\u306f\u5927\u8178\u83cc\u306e\u57f9\u990a\u306b\u4f7f\u7528\u3055\u308c\u308b\u306e\u304c\u4e00\u822c\u7684\u304b\u3068\u601d\u3044\u307e\u3059\u3002\u3053\u306e\u5bd2\u5929\u306f\u30a2\u30ac\u30ed\u30fc\u30b9\u3068\u30a2\u30ac\u30ed\u30da\u30af\u30c1\u30f3\u304b\u3089\u69cb\u6210\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u96fb\u6c17\u6cf3\u52d5\u304c\u5229\u7528\u3055\u308c\u59cb\u3081\u305f\u5f53\u521d\u306f\u3001\u5bd2\u5929\u3092\u4f7f\u3063\u305f\u96fb\u6c17\u6cf3\u52d5\u3082\u884c\u308f\u308c\u3066\u3044\u305f\u3088\u3046\u3067\u3059\u304c\u3001\u30a2\u30ac\u30ed\u30da\u30af\u30c1\u30f3\u306b\u786b\u9178\u57fa\u306a\u3069\u304c\u542b\u307e\u308c\u3066\u3044\u308b\u305f\u3081<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/cloning\/cloning-learning-center\/invitrogen-school-of-molecular-biology\/na-electrophoresis-education\/na-electrophoresis-considerations.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_basic_workshop_gsd_ts_1_Social_LAB\ufffd\" target=\"_blank\" rel=\"noopener\">\u96fb\u6c17\u6d78\u900f\u73fe\u8c61<\/a>\u3092\u8d77\u3053\u3057\u3066\u6cf3\u52d5\u3092\u59a8\u3052\u3066\u3044\u307e\u3057\u305f\u3002\u305d\u3053\u3067\u3001\u5bd2\u5929\u304b\u3089\u30a2\u30ac\u30ed\u30da\u30af\u30c1\u30f3\u3092\u9664\u3044\u3066\u30a2\u30ac\u30ed\u30fc\u30b9\u3092\u7cbe\u88fd\u3057\u3066\u96fb\u6c17\u6cf3\u52d5\u306b\u4f7f\u7528\u3057\u305f\u3068\u3053\u308d\u3001\u826f\u597d\u306a\u7d50\u679c\u3092\u5f97\u3089\u308c\u308b\u3088\u3046\u306b\u306a\u308a\u4eca\u65e5\u306e\u96fb\u6c17\u6cf3\u52d5\u306b\u307e\u3067\u3064\u306a\u304c\u3063\u3066\u3044\u307e\u3059\u3002<\/p>\n<p>\u3067\u306f\u3001\u5f53\u521d\u306e\u5bd2\u5929\u3092\u4f7f\u3063\u305f\u96fb\u6c17\u6cf3\u52d5\u50cf\u306f\u3001\u3069\u306e\u3088\u3046\u306a\u611f\u3058\u3060\u3063\u305f\u306e\u3067\u3057\u3087\u3046\u304b\uff1f\u307e\u305f\u3001\u30b2\u30eb\u306e\u6e96\u5099\u3092\u3059\u308b\u6bb5\u968e\u3067\u30a2\u30ac\u30ed\u30fc\u30b9\u306e\u5728\u5eab\u304c\u7121\u3044\u3053\u3068\u306b\u6c17\u4ed8\u3044\u3066\u3057\u307e\u3063\u305f\u975e\u5e38\u4e8b\u614b\u306e\u6642\u3067\u3082\u3001\u5927\u8178\u83cc\u57f9\u990a\u7528\u306e\u5bd2\u5929\u304c\u3042\u308c\u3070\u5b9f\u9a13\u3092\u7d99\u7d9a\u3067\u304d\u308b\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u3084\u3063\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n<h2>\u7d50\u679c<\/h2>\n<p>1%\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u30681%\u5bd2\u5929\uff08Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/30391023\/?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_basic_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Select Agar\u2122<\/a>\uff09\u3092\u305d\u308c\u305e\u308c\u7528\u610f\u3057\u307e\u3057\u305f\u3002\u540c\u3058\u6761\u4ef6\u306b\u3066\u3001PCR\u7523\u7269\uff08856 bp\uff09\u3068DNA\u30b5\u30a4\u30ba\u30de\u30fc\u30ab\u30fc\uff08Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/10787018\/?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_basic_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">1 Kb Plus DNA Ladder<\/a>\uff09\u3092\u6cf3\u52d5\u3057\u305f\u7d50\u679c\u304c\u3053\u3061\u3089\u3067\u3059\uff08\u56f31\uff09\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-13711\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-01.png\" alt=\"\u30a2\u30ac\u30ed\u30fc\u30b9\uff08\u5de6\uff09\u3068\u5bd2\u5929\uff08\u53f3\uff09\u3092\u4f7f\u7528\u3057\u305fDNA\u306e\u96fb\u6c17\u6cf3\u52d5\" width=\"3163\" height=\"1688\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-01.png 3163w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-01-300x160.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-01-1024x546.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-01-768x410.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-01-1536x820.png 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-01-2048x1093.png 2048w\" sizes=\"auto, (max-width: 3163px) 100vw, 3163px\" \/><\/p>\n<p>\u5de6\u5074\u304c\u901a\u5e38\u306e1%\u30a2\u30ac\u30ed\u30fc\u30b9\u3067\u3059\u304c\u3001\u30d0\u30c3\u30af\u30b0\u30e9\u30a6\u30f3\u30c9\u3082\u4f4e\u304f\u30de\u30fc\u30ab\u30fc\u306e\u30d0\u30f3\u30c9\u3082\u3084\u3084\u30b7\u30e3\u30fc\u30d7\u306a\u3088\u3046\u306b\u601d\u3044\u307e\u3059\u3002\u4e00\u65b9\u3067\u53f3\u5074\u306e1%\u5bd2\u5929\u3067\u306f\u30d0\u30f3\u30c9\u304c\u3084\u3084\u307c\u3084\u3051\u3066\u304a\u308a\u3001PCR\u7523\u7269\u306e\u6cf3\u52d5\u50cf\u304c\u4e71\u308c\u3066\u3057\u307e\u3044\u307e\u3057\u305f\u3002\u307e\u305f\u3001\u540c\u4e00\u6cf3\u52d5\u69fd\u306b\u3066\u540c\u6642\u306b\u540c\u6761\u4ef6\u3067\u6cf3\u52d5\u3057\u305f\u306b\u3082\u304b\u304b\u308f\u3089\u305a\u3001DNA\u306e\u30d0\u30f3\u30c9\u306e\u79fb\u52d5\u5ea6\u304c\u7570\u306a\u3063\u3066\u3044\u307e\u3057\u305f\u3002\u5bd2\u5929\u306e\u65b9\u304c\u79fb\u52d5\u5ea6\u304c\u4f4e\u4e0b\u3057\u305f\u306e\u306f\u3001\u30a2\u30ac\u30ed\u30da\u30af\u30c1\u30f3\u306e\u786b\u9178\u57fa\u306a\u3069\u306b\u3088\u308a\u96fb\u6c17\u6d78\u900f\u306e\u5f71\u97ff\u304c\u3042\u3063\u305f\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u305f\u3060\u3001\u79fb\u52d5\u5ea6\u306e\u9055\u3044\u306f\u3042\u308a\u307e\u3057\u305f\u304c\u3001\u5bd2\u5929\u30b2\u30eb\u3067\u3082\u30d0\u30f3\u30c9\u306e\u691c\u51fa\u306f\u3067\u304d\u30011960\u5e74\u4ee3\u306b\u601d\u3044\u3092\u306f\u305b\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3057\u305f\u3002<\/p>\n<p>\u5b9f\u306f\u3001\u56f31\u306e\u7d50\u679c\u306f2\u56de\u76ee\u306e\u30c1\u30e3\u30ec\u30f3\u30b8\u3067\u3057\u305f\u30021\u56de\u76ee\u3067\u306f\u5bd2\u5929\u30b2\u30eb\u3092\u5272\u3063\u3066\u3057\u307e\u3063\u305f\u306e\u3067\u3057\u305f\u3002\u30a2\u30ac\u30ed\u30fc\u30b9\u3082\u5bd2\u5929\u3082\u3001\u6eb6\u304b\u3059\u6642\u3084\u30c8\u30ec\u30fc\u306b\u6d41\u3057\u5165\u308c\u308b\u6642\u306a\u3069\u306e\u30b2\u30eb\u4f5c\u6210\u904e\u7a0b\u3067\u306f\u5927\u304d\u306a\u9055\u3044\u3092\u611f\u3058\u306a\u304b\u3063\u305f\u306e\u3067\u3059\u304c\u3001\u624b\u306b\u6301\u3063\u3066\u307f\u305f\u6642\u306b\u786c\u3055\u3068\u30c4\u30eb\u30c4\u30eb\u5177\u5408\u306b\u82e5\u5e72\u306e\u9055\u548c\u611f\u3092\u611f\u3058\u305f\u306e\u3067\u614e\u91cd\u306b\u4f5c\u696d\u3057\u307e\u3057\u305f\u3002\u305d\u306e\u305f\u3081\u3001\u5bd2\u5929\u30b2\u30eb\u306e\u6271\u3044\u306b\u306f\u6c17\u3092\u4ed8\u3051\u3066\u3044\u305f\u3064\u3082\u308a\u3067\u3057\u305f\u304c\u3001\u305b\u3063\u304b\u304f\u96fb\u6c17\u6cf3\u52d5\u307e\u3067\u884c\u3063\u305f\u30b2\u30eb\u304c\u6ed1\u3063\u3066\u843d\u3061\u3066\u3057\u307e\u3044\u3001\u3042\u3063\u3055\u308a\u5272\u308c\u3066\u3057\u307e\u3044\u307e\u3057\u305f\uff08\u56f32\uff09\u3002\u79c1\u306f\u3055\u307e\u3056\u307e\u306a\u6fc3\u5ea6\u306e\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u3092\u53d6\u308a\u6271\u3063\u3066\u304d\u307e\u3057\u305f\u304c\u3001\u5272\u3063\u3066\u3057\u307e\u3046\u3053\u3068\u306f\u307b\u3068\u3093\u3069\u3042\u308a\u307e\u305b\u3093\u3067\u3057\u305f\u3002\u30a2\u30ac\u30ed\u30fc\u30b9\u3068\u6bd4\u8f03\u3057\u3066\u5bd2\u5929\u306f\u30b2\u30eb\u5f37\u5ea6\u304c\u4f4e\u3044\u306e\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u30d1\u30ba\u30eb\u306e\u3088\u3046\u306b\u5fa9\u5143\u3057\u3088\u3046\u3068\u3057\u307e\u3057\u305f\u304c\u3001\u3046\u307e\u304f\u3067\u304d\u307e\u305b\u3093\u3067\u3057\u305f\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-13712\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-02.png\" alt=\"\u5bd2\u5929\u306e\u30b2\u30eb\u306f\u58ca\u308c\u3084\u3059\u304b\u3063\u305f\" width=\"472\" height=\"483\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-02.png 472w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/03\/basic-workshop-gsd-ts-1-02-293x300.png 293w\" sizes=\"auto, (max-width: 472px) 100vw, 472px\" \/><\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<p>\u30a2\u30ac\u30ed\u30fc\u30b9\u306e\u30e9\u30dc\u5728\u5eab\u304c\u7121\u304f\u306a\u3063\u3066\u3044\u3066\u3082\u3001\u5927\u8178\u83cc\u7528\u306e\u5bd2\u5929\u3067\u4ee3\u7528\u3059\u308b\u3053\u3068\u3067\u30d0\u30f3\u30c9\u306e\u6709\u7121\u3092\u78ba\u8a8d\u3059\u308b\u304f\u3089\u3044\u306f\u53ef\u80fd\u306a\u3053\u3068\u304c\u5206\u304b\u308a\u307e\u3057\u305f\u3002\u4eca\u56de\u306f\u5f53\u793e\u306e\u57f9\u5730\u7528\u88fd\u54c1\u306e\u5bd2\u5929\uff08<a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/30391023\/?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_basic_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" rel=\"noopener\">Select Agar\u2122<\/a>\uff09\u3092\u4f7f\u7528\u3057\u307e\u3057\u305f\u304c\u3001\u30b9\u30fc\u30d1\u30fc\u30de\u30fc\u30b1\u30c3\u30c8\u306a\u3069\u3067\u5e02\u8ca9\u3055\u308c\u3066\u3044\u308b\u5bd2\u5929\u3067\u3082\u540c\u69d8\u306e\u7d50\u679c\u304c\u5f97\u3089\u308c\u308b\u306e\u304b\u6c17\u306b\u306a\u308b\u3068\u3053\u308d\u3067\u3059\u3002<\/p>\n<p>\u5f53\u793e\u3067\u306f\u3001\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u3084PCR\u306a\u3069\u5b9f\u969b\u306b\u5b9f\u9a13\uff08\u5b9f\u7fd2\uff09\u3092\u884c\u3044\u3064\u3064\u5b66\u3079\u308b\u5404\u7a2e<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/technical-resources\/trainings\/lifescience-training.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_og_s00_basic_workshop_gsd_ts_1_Social_LAB\" target=\"_blank\" 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rel=\"noopener\">\u6838\u9178\u96fb\u6c17\u6cf3\u52d5\u306e\u30c8\u30e9\u30d6\u30eb\u30b7\u30e5\u30fc\u30c6\u30a3\u30f3\u30b0\u30ac\u30a4\u30c9<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30a2\u30ac\u30ed\u30fc\u30b9\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306f\u3001DNA\u306a\u3069\u306e\u6838\u9178\u3092\u691c\u51fa\u3059\u308b\u4e00\u822c\u7684\u306a\u65b9\u6cd5\u3067\u3059\u3002\u305d\u306e\u6b74\u53f2\u306f1960\u5e74\u4ee3\u5f8c\u534a\u306b\u307e\u3067\u3055\u304b\u306e\u307c\u308b\u3053\u3068\u304c\u3067\u304d\u307e\u3059\uff08\u6838\u9178\u30b2\u30eb\u96fb\u6c17\u6cf3\u52d5\u306e\u6b74\u53f2\uff09\u30021960\u5e74\u4ee3\u521d\u982d\u306e\u96fb\u6c17\u6cf3\u52d5\u3067\u306f\u6eb6\u6db2\u4e2d\u3067DNA\u306e\u79fb\u52d5\u5ea6\u3092\u8abf\u3079\u3066\u3044\u305f\u3088<\/p>\n","protected":false},"author":975,"featured_media":13711,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"_kad_blocks_custom_css":"","_kad_blocks_head_custom_js":"","_kad_blocks_body_custom_js":"","_kad_blocks_footer_custom_js":"","_monsterinsights_skip_tracking":false,"_genesis_hide_title":false,"_genesis_hide_breadcrumbs":false,"_genesis_hide_singular_image":false,"_genesis_hide_footer_widgets":false,"_genesis_custom_body_class":"","_genesis_custom_post_class":"","_genesis_layout":"","_jetpack_newsletter_access":"","_jetpack_dont_email_post_to_subs":false,"_jetpack_newsletter_tier_id":0,"_jetpack_memberships_contains_paywalled_content":false,"_jetpack_feature_clip_id":0,"_jetpack_memberships_contains_paid_content":false,"footnotes":"","jetpack_post_was_ever_published":false},"categories":[156],"tags":[9,28],"division":[],"class_list":{"0":"post-13708","1":"post","2":"type-post","3":"status-publish","4":"format-standard","5":"has-post-thumbnail","7":"category-mol-bio","8":"tag-pcr","9":"tag-experiment","10":"entry"},"_selected_authors":"","_selected_reviewers":"","acf":[],"yoast_head":"<!-- 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