{"id":1380,"date":"2018-09-30T00:00:00","date_gmt":"2018-09-29T15:00:00","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_42_1_quantstudio\/"},"modified":"2025-02-27T17:00:57","modified_gmt":"2025-02-27T08:00:57","slug":"user_voice_42_1_quantstudio","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/user_voice_42_1_quantstudio\/","title":{"rendered":"\u8840\u6f3f\u904a\u96e2DNA\u304b\u3089\u53bb\u52e2\u62b5\u6297\u6027\u524d\u7acb\u817a\u304c\u3093\u306e\u7279\u5fb4\u3092\u6349\u3048\u308b\uff08\u4eac\u90fd\u5927\u5b66\u533b\u5b66\u7814\u7a76\u79d1 \u4f4f\u5409\u5d07\u5e78\u6c0f\uff09-\u300cNEXT\u300d2017\u5e7410\u6708\u53f7\u63b2\u8f09"},"content":{"rendered":"<h2><span style=\"font-size: 12pt\">QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3067\u30a2\u30f3\u30c9\u30ed\u30b2\u30f3\u53d7\u5bb9\u4f53\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u5909\u7570\u3092\u691c\u51fa<\/span><\/h2>\n<p><strong>\u4f4f\u5409\u5d07\u5e78 \u6c0f \uff08\u4eac\u90fd\u5927\u5b66\u5927\u5b66\u9662\u533b\u5b66\u7814\u7a76\u79d1\u6ccc\u5c3f\u5668\u79d1\u5b66\u5206\u91ce\uff09<\/strong><\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-3-2.jpg\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-4495\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/66c19942ab4ba346fdb64ccc04cde373-3-2.jpg\" alt=\"\u4f4f\u5409\u5d07\u5e78 \u6c0f \uff08\u4eac\u90fd\u5927\u5b66\u5927\u5b66\u9662\u533b\u5b66\u7814\u7a76\u79d1\u6ccc\u5c3f\u5668\u79d1\u5b66\u5206\u91ce\uff09\" width=\"250\" height=\"154\" \/><\/a>\u5d29\u58ca\u3057\u305f\u816b\u760d\u7d30\u80de\u304b\u3089\u653e\u51fa\u3055\u308c\u8840\u4e2d\u3092\u5faa\u74b0\u3059\u308b\u8840\u6f3f\u904a\u96e2DNA(cfDNA)\u306b\u6ce8\u76ee\u3057\u3001\u53bb\u52e2\u62b5\u6297\u6027\u524d\u7acb\u817a\u304c\u3093(CRPC)\u306e\u6cbb\u7642\u6cd5\u9078\u629e\u306b\u6d3b\u304b\u3059\u53ef\u80fd\u6027\u3092\u691c\u8a3c\u4e2d\u306e\u4eac\u90fd\u5927\u5b66\u533b\u5b66\u7814\u7a76\u79d1\u306e\u4f4f\u5409\u5d07\u5e78\u6c0f\u3002Applied Biosystems\u2122 QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3092\u4f7f\u3044\u3001CRPC\u60a3\u8005\u306e\u3054\u304f\u5fae\u91cf\u306ecfDNA\u304b\u3089\u30a2\u30f3\u30c9\u30ed\u30b2\u30f3\u53d7\u5bb9\u4f53(<i>AR)<\/i>\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u5909\u7570(CNV)\u3092\u691c\u51fa\u3057\u3001\u75c5\u539f\u56e0\u5b50\u306e\u89e3\u6790\u3092\u9032\u3081\u3066\u3044\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u3054\u304f\u50c5\u304b\u306a\u30b2\u30ce\u30e0\u65ad\u7247\u304b\u3089<i>AR<\/i>\u306e\u30b3\u30d4\u30fc\u6570\u5897\u5e45\u3092\u5272\u308a\u51fa\u3059<\/span><\/h2>\n<p>\u300cCRPC\u3068\u306f\u3001\u30a2\u30f3\u30c9\u30ed\u30b2\u30f3\u9664\u53bb\u6cbb\u7642\u306b\u3088\u308b\u53bb\u52e2\u72b6\u614b\u306b\u3088\u308a\u3001\u8840\u6e05\u4e2d\u306e\u30c6\u30b9\u30c8\u30b9\u30c6\u30ed\u30f3\u6fc3\u5ea6\u304c\u4f4e\u3044\u306b\u3082\u304b\u304b\u308f\u3089\u305a\u75c5\u52e2\u304c\u5897\u60aa\u3057\u3066\u3044\u308b\u30b1\u30fc\u30b9\u3092\u6307\u3057\u307e\u3059\u3002CPRC\u3067\u5341\u5206\u306a\u6cbb\u7642\u52b9\u679c\u3092\u78ba\u4fdd\u3059\u308b\u305f\u3081\u306b\u6b20\u304b\u305b\u306a\u3044\u306e\u304c\u3001\u8a73\u7d30\u306a\u75c5\u539f\u56e0\u5b50\u306e\u89e3\u6790\u300d\u3068\u4f4f\u5409\u6c0f\u3002\u3055\u3089\u306b<i>AR<\/i>\u907a\u4f1d\u5b50\u306e\u5909\u7570\u3092\u89e3\u6790\u3059\u308b\u81e8\u5e8a\u4e0a\u306e\u610f\u7fa9\u306b\u3064\u3044\u3066\u6b21\u306e\u69d8\u306b\u7d9a\u3051\u307e\u3059\u3002\u300cCRPC\u306e\u6cbb\u7642\u65b9\u91dd\u306fAR\u7cfb\u3092\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u3057\u305f\u85ac\u5264\u3092\u4f7f\u3046\u304b\u6297\u304c\u3093\u5264\u3092\u4f7f\u3046\u304b\u306e\u5927\u5225\u3057\u30662\u901a\u308a\u3067\u3059\u3002<i>AR<\/i>\u907a\u4f1d\u5b50\u306b\u5897\u5e45\u3084\u5909\u7570\u304c\u8d77\u304d\u3066\u3044\u308c\u3070\u3001AR\u30b7\u30b0\u30ca\u30eb\u7cfb\u3092\u30d6\u30ed\u30c3\u30af\u3059\u308b\u3088\u308a\u6297\u304c\u3093\u5264\u3092\u512a\u5148\u3059\u308b\u306a\u3069\u3068\u5224\u65ad\u6750\u6599\u306b\u306a\u308a\u307e\u3059\u300d\u3002\u5b9f\u969b\u306bCRPC\u60a3\u8005\u306e\u304a\u3088\u305d3-4\u5272\u306b<i>AR<\/i>\u907a\u4f1d\u5b50\u306e\u30b3\u30d4\u30fc\u6570\u5897\u5e45\u30012\u5272\u306b\u5909\u7570\u304c\u751f\u3058\u3066\u3044\u308b\u3068\u60f3\u5b9a\u3055\u308c\u307e\u3059\u3002\u300c\u3057\u304b\u3057\u4fb5\u8972\u6027\u306e\u9ad8\u3044\u30d0\u30a4\u30aa\u30d7\u30b7\u30fc\u306f\u65e5\u5e38\u8a3a\u7642\u3067\u306f\u884c\u308f\u306a\u3044\u306e\u3067\u3001\u8840\u6db2\u4e2d\u306ecfDNA\u304b\u3089\u30c7\u30b8\u30bf\u30ebPCR\u6cd5\u3067\u9ad8\u611f\u5ea6\u306b\u75c5\u539f\u5909\u7570\u3092\u691c\u51fa\u3067\u304d\u308c\u3070\u975e\u5e38\u306b\u6709\u7528\u3067\u3059\u300d\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u4e07\u5168\u306e\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8\u3067\u89e3\u6790\u3082\u5b89\u5fc3<\/span><\/h2>\n<p>CNV\u89e3\u6790\u3067\u96e3\u3057\u3044\u306e\u304c\u3001\u30ab\u30c3\u30c8\u30aa\u30d5\u5024\u306e\u8a2d\u5b9a\u3002\u4eca\u56de\u306f\u3069\u306e\u3088\u3046\u306b\u8a2d\u5b9a\u3055\u308c\u305f\u306e\u3067\u3057\u3087\u3046\u304b\u3002\u300c\u89e3\u6790\u9818\u57df\u306fAR\u30ea\u30ac\u30f3\u30c9\u7d50\u5408\u90e8\u4f4d\u306e\u30a4\u30f3\u30c8\u30ed\u30f3\u306b\u3057\u307e\u3057\u305f\u3002<i>AR\u907a\u4f1d\u5b50<\/i>\u306fX\u67d3\u8272\u4f53\u4e0a\u306b\u3042\u308b\u306e\u3067\u3001\u5bfe\u7167\u306e<i>RNaseP<\/i>\u907a\u4f1d\u5b50\u306e\u5b58\u5728\u91cf\u30921\u3068\u3059\u308c\u3070AR\u306f0.5\u306e\u306f\u305a\u3002\u305d\u3053\u3067\u5065\u5e38\u7537\u6027\u306e\u5024\u306b\u30d7\u30e9\u30b92SD\u3068\u306a\u308b0.8\u3092\u30ab\u30c3\u30c8\u30aa\u30d5\u5024\u306b\u8a2d\u5b9a\u3002\u305d\u306e\u7d50\u679c\u3001\u7d0480\u4f8b\u306eCRPC\u60a3\u8005\u306e3\u5272\u5f37\u306b\u30b3\u30d4\u30fc\u6570\u5897\u5e45\u304c\u307f\u3089\u308c\u308b\u3053\u3068\u3092\u78ba\u8a8d\u3067\u304d\u307e\u3057\u305f\u300d\u3068\u4f4f\u5409\u6c0f\u306f\u4eca\u56de\u306e\u7d50\u679c\u3092\u8aac\u660e\u3057\u307e\u3059\u3002\u307e\u305f\u3001\u89e3\u6790\u524d\u306b\u306f\u5f0a\u793e\u306e\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8\u306b\u3082\u76f8\u8ac7\u3057\u305f\u305d\u3046\u3067\u3059\u3002\u300cTaqMan\u30a2\u30c3\u30bb\u30a4\u306e\u8a2d\u8a08\u3084\u30c7\u30fc\u30bf\u89e3\u6790\u306f\u6163\u308c\u306a\u3044\u3068\u4e0d\u5b89\u306a\u3053\u3068\u3082\u591a\u3044\u306e\u3067\u3059\u304c\u3001\u304d\u3081\u7d30\u304b\u3044\u30b5\u30dd\u30fc\u30c8\u306e\u304a\u304b\u3052\u3067\u30b9\u30e0\u30fc\u30ba\u3067\u304d\u307e\u3057\u305f\u3002\u65b0\u3057\u3044\u6280\u8853\u306a\u306e\u3067\u3001\u3053\u306e\u3088\u3046\u306a\u652f\u63f4\u306f\u3068\u3066\u3082\u52a9\u304b\u308a\u307e\u3057\u305f\u300d\u3068\u4f4f\u5409\u6c0f\u3002<br \/>\n\u300c\u4eca\u5f8c\u306f\u30b3\u30d4\u30fc\u6570\u5909\u7570\u3088\u308a\u3082\u3055\u3089\u306b\u4f4e\u983b\u5ea6\u306e<i>AR\u907a\u4f1d\u5b50<\/i>\u5909\u7570\u306e\u89e3\u6790\u3082\u9032\u3081\u308b\u4e88\u5b9a\u3067\u3059\u3002cfDNA\u306f\u30a4\u30f3\u30d7\u30c3\u30c8\u91cf\u304c\u6975\u5fae\u91cf\u306a\u306e\u3067\u5909\u7570\u691c\u51fa\u306f\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u30b7\u30b9\u30c6\u30e0\u3067\u306f\u307b\u307c\u4e0d\u53ef\u80fd\u3067\u3042\u308a\u3001\u6b21\u4e16\u4ee3\u30b7\u30fc\u30b1\u30f3\u30b9\u3084\u30c7\u30b8\u30bf\u30ebPCR\u3067\u884c\u3063\u3066\u3044\u304d\u307e\u3059\u3002\u307e\u305f\u672b\u68a2\u8840\u5faa\u74b0\u816b\u760d\u7d30\u80de(CTC)\u3092\u7528\u3044\u305f\u7814\u7a76\u306b\u3082\u53d6\u308a\u7d44\u307f\u305f\u3044\u300d\u3068\u8a71\u3057\u307e\u3059\u3002CTC\u7531\u6765\u306ecDNA\u3084cfDNA\u306e\u3088\u3046\u306a\u6975\u5fae\u91cf\u306e\u907a\u4f1d\u5b50\u60c5\u5831\u3092\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3067\u9ad8\u611f\u5ea6\u306b\u89e3\u6790\u3059\u308b\u3001\u6b21\u4e16\u4ee3\u306e\u57fa\u790e\u7814\u7a76\u304c\u9032\u3093\u3067\u3044\u307e\u3059\u3002<\/p>\n<h2><span style=\"font-size: 12pt\">\u5fae\u91cf\u6838\u9178\u304b\u3089\u30b3\u30d4\u30fc\u6570\u591a\u578b(CNV)\u3092\u9ad8\u611f\u5ea6\u306b\u691c\u51fa<\/span><br \/>\n<span style=\"font-size: 12pt\">QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr.html?CID=Social_LAB\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3704 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/p9_QuantStudio_3d_MG_0754_Front_rotate-10in-6.jpg\" alt=\"\" width=\"200\" height=\"149\" \/><\/a>QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306f\u3001\u30b3\u30f3\u30d1\u30af\u30c8\u3067\u4f7f\u3044\u3084\u3059\u3044\u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u3067\u3059\u3002CNV\u89e3\u6790\u3067\u306f\u3001Applied Biosystems\u2122 TaqMan\u00ae Copy Number Assays\u3068\u3057\u3066\u3001\u30a4\u30f3\u30c8\u30ed\u30f3\u3092\u542b\u3080\u5168\u30b2\u30ce\u30e0\u9818\u57df\u306b\u5bfe\u3057\u3066\u30d2\u30c8\u3067\u306f160\u4e07\u7a2e\u985e\u3001\u30de\u30a6\u30b9\u3067\u306f18\u4e07\u7a2e\u985e\u306e\u30c7\u30b6\u30a4\u30f3\u6e08\u307f\u30a2\u30c3\u30bb\u30a4\u3092\u63d0\u4f9b\u4e2d\u3067\u3059\u3002<\/p>\n<p>&nbsp;<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr5n_Social_LAB\" target=\"_blank\" rel=\"noopener\">QuantStudio 3D \u30c7\u30b8\u30bf\u30ebPCR\u30b7\u30b9\u30c6\u30e0\u306e\u8a73\u7d30\u60c5\u5831\u3084\u30c7\u30e2\u306e\u304a\u7533\u8fbc\u307f\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n<h2><span style=\"font-size: 12pt\">\u9ad8\u611f\u5ea6\u306a\u907a\u4f1d\u5b50\u5909\u7570\u89e3\u6790\u3092\u5b9f\u73fe!<\/span><br \/>\n<span style=\"font-size: 12pt\">TaqMan dPCR Liquid Biopsy Assays<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr\/sensitive-mutation-detection-taqman-liquid-biopsy-dpcr-assays.html?CID=Social_LAB\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft wp-image-3865 size-full\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/p18_.jpg\" alt=\"TaqMan dPCR Liquid Biopsy Assays\" width=\"250\" height=\"166\" \/><\/a>Applied Biosystems\u2122 TaqMan\u00ae dPCR Liquid Biopsy Assays \u306f\u3001\u304c\u3093\u7814\u7a76\u306b\u6b20\u304b\u305b\u306a\u3044\u304c\u3093\u95a2\u9023\u907a\u4f1d\u5b50\uff08<i>EGFR, BRAF,KRAS, PIK3CA, JAK2<\/i> ,\u4ed6\uff09\u306e\u5909\u7570\u3092\u611f\u5ea6\u3088\u304f\u691c\u51fa\u3059\u308b\u30d0\u30ea\u30c7\u30fc\u30b7\u30e7\u30f3\u6e08\u307f\u306e\u30a2\u30c3\u30bb\u30a4\u30b7\u30ea\u30fc\u30ba\u30020.1%\u7a0b\u5ea6\u306e\u5909\u7570\u3092\u691c\u51fa\u3057\u307e\u3059\u3002<\/p>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/digital-pcr\/quantstudio-3d-applications\/sensitive-mutation-detection-taqman-liquid-biopsy-dpcr-assays.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr5n_Social_LAB\" target=\"_blank\" rel=\"noopener\">TaqMan\u00ae dPCR Liquid Biopsy Assays\u306e\u8a73\u7d30\u60c5\u5831\u3084\u30c7\u30e2\u306e\u304a\u7533\u8fbc\u307f\u306f\u3053\u3061\u3089\u304b\u3089<\/a><\/p>\n<h2><span style=\"font-size: 12pt\">\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d<\/span><\/h2>\n<p><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=Social_LAB\"><img loading=\"lazy\" decoding=\"async\" class=\"alignleft size-full wp-image-3867\" src=\"https:\/\/www.thermofisher.com\/blog\/wp-content\/uploads\/sites\/13\/2019\/07\/foregroundimg.img_.full_.high_-3-1.png\" alt=\"\u300cNEXT\u300d2017\u5e7410\u6708\u53f7\" width=\"167\" height=\"235\" \/><\/a>\u5f53\u8a18\u4e8b\u306f\u30b5\u30fc\u30e2\u30d5\u30a3\u30c3\u30b7\u30e3\u30fc\u30b5\u30a4\u30a8\u30f3\u30c6\u30a3\u30d5\u30a3\u30c3\u30af\u304c\u767a\u520a\u3059\u308b\u30e9\u30a4\u30d5\u30b5\u30a4\u30a8\u30f3\u30b9\u60c5\u5831\u8a8c\u300cNEXT\u300d2017\u5e7410\u6708\u53f7\u304b\u3089\u306e\u629c\u7c8b\u3067\u3059\u3002<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/communities-social\/NEXT-Forum\/next\/next-backnumber.html?CID=gsd_pcr_sbu_r04_jp_cp0000_pjt0000_gsd00000_0so_blg_op_awa_oc_s00_qpcr5n_Social_LAB\" target=\"_blank\" 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