{"id":13935,"date":"2023-05-08T15:35:55","date_gmt":"2023-05-08T06:35:55","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=13935"},"modified":"2025-02-10T14:28:38","modified_gmt":"2025-02-10T05:28:38","slug":"gene-editing_bid_ts_3","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_3\/","title":{"rendered":"\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c3\u56de\u30003\u3064\u306eCRIPSR\/Cas9\u5b9f\u9a13\u7cfb\uff5e"},"content":{"rendered":"<p>\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u521d\u5fc3\u8005A\u3055\u3093\u306b\u3088\u308b\u300c\u521d\u3081\u3066\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u300d\u3002<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_1\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c1\u56de<\/a>\u3001<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_2\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u7b2c2\u56de<\/a>\u3092\u901a\u3057\u3066\u57fa\u790e\u7684\u306a\u5185\u5bb9\u3092\u7406\u89e3\u3057\u305fA\u3055\u3093\u306f\u3001\u3044\u3088\u3044\u3088\u5b9f\u9a13\u3092\u59cb\u3081\u3088\u3046\u3068\u3057\u3066\u3044\u307e\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-13986\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-300x160.png\" alt=\"\" width=\"500\" height=\"267\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-300x160.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-1024x546.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-768x410.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01-1536x819.png 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-01.png 1787w\" sizes=\"auto, (max-width: 500px) 100vw, 500px\" \/><\/p>\n<div class=\"voice-text-right\">\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u30ac\u30a4\u30c9RNA\u304c\u30c7\u30b6\u30a4\u30f3\u3067\u304d\u307e\u3057\u305f\uff01<br \/>\n\u3053\u308c\u3067\u57f9\u990a\u7d30\u80de\u306e\u30ce\u30c3\u30af\u30a2\u30a6\u30c8\u5b9f\u9a13\u304c\u3067\u304d\u307e\u3059\u306d<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u5f85\u3063\u3066\u3002\u305d\u306e\u524d\u306b<b>CIRSPR\/Cas9\u306e3\u3064\u306e\u5b9f\u9a13\u7cfb<\/b>\u304b\u3089\u3061\u3083\u3093\u3068\u9078\u3093\u3060\uff1f<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n3\u3064\u306e\u5b9f\u9a13\u7cfb\u3063\u3066\uff1f<\/p>\n<p>\u4f9b\u4e0e\u65b9\u6cd5\u3001\u3064\u307e\u308a\u300cCas9\u3068\u30ac\u30a4\u30c9RNA\u3092\u3069\u3046\u3084\u3063\u3066\u7d30\u80de\u306b\u4e0e\u3048\u308b\u304b\u300d\u306b\u306f\u30013\u3064\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002\u5b9f\u9a13\u3092\u884c\u3046\u524d\u306b\u3001\u3053\u306e3\u7a2e\u985e\u306e\u3069\u306e\u65b9\u6cd5\u3067\u5b9f\u65bd\u3059\u308b\u304b\u3001\u9078\u629e\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n<ul>\n<li>DNA\uff08Vector\uff09\u65b9\u6cd5\uff1a<br \/>\n\u7b2c\u4e00\u4e16\u4ee3\u3002Cas9\u3068\u30ac\u30a4\u30c9RNA\u4e21\u65b9\u306e\u914d\u5217\u3092DNA\uff08Vector\uff09\u306b\u7d44\u307f\u8fbc\u3093\u3067\u3001\u7d30\u80de\u306b\u4f9b\u4e0e\u3059\u308b\u65b9\u6cd5<\/li>\n<li>RNA\uff08Cas9 mRNA\uff09\u65b9\u6cd5\uff1a<br \/>\n\u7b2c\u4e8c\u4e16\u4ee3\u3002Cas9\u3068\u30ac\u30a4\u30c9RNA\u3092\u3001\u305d\u308c\u305e\u308cRNA\u306e\u72b6\u614b\u3067\u7d30\u80de\u306b\u4f9b\u4e0e\u3059\u308b\u65b9\u6cd5<\/li>\n<li>Protein\uff08Cas9 protein\uff09\u65b9\u6cd5\uff1a<br \/>\nCas9 \u3092Cas9 \u30bf\u30f3\u30d1\u30af\u8cea\u3001\u30ac\u30a4\u30c9RNA\u3092RNA\u3068\u3057\u3066\u7d30\u80de\u306b\u4f9b\u4e0e\u3059\u308b\u65b9\u6cd5<\/li>\n<\/ul>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u3064\u307e\u308a\u3001\u6750\u6599\u306e\u4f5c\u308a\u65b9\u304c\u9055\u3046\u3093\u3067\u3059\u306d\u3002<br \/>\n\u3063\u3066\u3053\u3068\u306f\u3001\u7d30\u80de\u5185\u306e\u52d5\u304d\u3082\u9055\u3046\u3093\u3067\u3059\u304b\uff1f<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u3044\u3044\u8cea\u554f\u3060\u306d\u3002\u56f31\u3092\u898b\u3066\u307f\u3088\u3046\u30023\u3064\u306e\u65b9\u6cd5\u306e\u7d30\u80de\u5185\u6319\u52d5\u306e\u9055\u3044\u304c\u8aac\u660e\u3057\u3066\u3042\u308b\u3088<\/p>\n<div id=\"attachment_13982\" style=\"width: 497px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-13982\" class=\"wp-image-13982 size-full\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-02.png\" alt=\"3\u3064\u306e\u65b9\u6cd5\u3067\u5c0e\u5165\u3055\u308c\u305fCas9\u3068\u30ac\u30a4\u30c9RNA\u304c\u7d30\u80de\u5185\u3067\u6838\u5185\u306b\u79fb\u884c\u3059\u308b\u307e\u3067\" width=\"487\" height=\"291\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-02.png 487w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-02-300x179.png 300w\" sizes=\"auto, (max-width: 487px) 100vw, 487px\" \/><p id=\"caption-attachment-13982\" class=\"wp-caption-text\">\u56f31\uff1a 3\u3064\u306e\u65b9\u6cd5\u3067\u5c0e\u5165\u3055\u308c\u305fCas9\u3068\u30ac\u30a4\u30c9RNA\u304c<br \/>\u7d30\u80de\u5185\u3067\u6838\u5185\u306b\u79fb\u884c\u3059\u308b\u307e\u3067<\/p><\/div>\n<p>DNA\uff08Vector\uff09\u65b9\u6cd5\u3067\u306f\u3001vector\u304c\u7d30\u80de\u5185\u306b\u5c0e\u5165\u3055\u308c\u308b\u3068\u3001\u6838\u306b\u79fb\u884c\u3057\u305fvector\u304b\u3089\u30ac\u30a4\u30c9RNA\u3068Cas9 mRNA\u304c\u8ee2\u5199\u3055\u308c\u3001\u7d30\u80de\u8cea\u3067Cas9 \u30bf\u30f3\u30d1\u30af\u8cea\u304c\u7ffb\u8a33\u3055\u308c\u307e\u3059\u3002\u305d\u306e\u5f8c\u3001\u7d30\u80de\u8cea\u5185\u3067\u30ac\u30a4\u30c9RNA\u3068Cas9\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30b3\u30f3\u30d7\u30ec\u30c3\u30af\u30b9\u3092\u5f62\u6210\u3057\u3066\u3001Cas9 protein\u306e\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u306b\u3088\u3063\u3066\u6838\u5185\u306b\u79fb\u884c\u3057\u307e\u3059\u3002<br \/>\nRNA\u65b9\u6cd5\u306f\u3001\u30ac\u30a4\u30c9RNA\u3068Cas9 mRNA\u3092\u540c\u6642\u306b\u5c0e\u5165\u3057\u307e\u3059\u3002\u7d30\u80de\u8cea\u306b\u5c0e\u5165\u3055\u308c\u305fCas9 mRNA\u304cCas9\u30bf\u30f3\u30d1\u30af\u8cea\u306b\u7ffb\u8a33\u3055\u308c\u3001\u30ac\u30a4\u30c9RNA\u3068\u30b3\u30f3\u30d7\u30ec\u30c3\u30af\u30b9\u3092\u5f62\u6210\u3057\u3066\u3001\u6838\u306b\u79fb\u884c\u3057\u307e\u3059\u3002<br \/>\nProtein\u65b9\u6cd5\u3067\u3082\u3001\u30ac\u30a4\u30c9RNA\u3068Cas9\u30bf\u30f3\u30d1\u30af\u8cea\u3092\u540c\u6642\u306b\u5c0e\u5165\u3057\u307e\u3059\u3002\u7d30\u80de\u8cea\u306b\u5c0e\u5165\u3055\u308c\u305f\u30ac\u30a4\u30c9RNA\u3068Cas9\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u30b3\u30f3\u30d7\u30ec\u30c3\u30af\u30b9\u3092\u5f62\u6210\u5f8c\u3001\u6838\u306b\u79fb\u884c\u3057\u307e\u3059\u3002<br \/>\n3\u3064\u306e\u65b9\u6cd5\u3044\u305a\u308c\u3082\u3001\u6838\u5185\u79fb\u884c\u5f8c\u306f\u30ac\u30a4\u30c9RNA\u304c\u76ee\u7684\u907a\u4f1d\u5b50\u306b\u7d50\u5408\u3057\u3066\u3001\u30b2\u30ce\u30e0\u3092\u5207\u65ad\u3057\u307e\u3059\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u306a\u308b\u307b\u3069\u3002<br \/>\n\u3067\u3001\u7d50\u5c40\u3069\u308c\u304c\u4e00\u756a\u304a\u52e7\u3081\u306a\u3093\u3067\u3059\u304b\uff1f<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u3046\u3093\u3001\u305d\u308c\u304c\u77e5\u308a\u305f\u3044\u3088\u306d\u3002\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u52b9\u7387\u304c\u9ad8\u3044\u65b9\u6cd5\u306f\u3001Protein\u65b9\u6cd5\u3060\u3088<\/p>\n<p>\u300c\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u304c\u9ad8\u3044\u300d\u3068\u306f\u3001\u300c\u5207\u65ad\u3057\u305f\u5f8c\u306b\u5909\u7570\u304c\u5165\u308b\u52b9\u7387\u304c\u9ad8\u3044\u300d\u3053\u3068\u3092\u6307\u3057\u307e\u3059\u3002<br \/>\n\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u304c\u9ad8\u3044\u65b9\u6cd5\u306f\u3001Protein\u65b9\u6cd5\u3067\u3059\u3002<br \/>\n\u7b2c2\u4e16\u4ee3\u306eRNA\u65b9\u6cd5\u306fDNA\uff08Vector\uff09\u65b9\u6cd5\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u3092\u6539\u5584\u3057\u305f\u65b9\u6cd5\u306e\u305f\u3081\u3001DNA\u65b9\u6cd5\u3088\u308a\u3082RNA\u65b9\u6cd5\u306e\u65b9\u304c\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u306f\u9ad8\u3044\u3067\u3059\u3002<\/p>\n<p><span style=\"color: #3366ff\">A\u3055\u3093<\/span>\uff1a<br \/>\n\u3068\u3053\u308d\u3067\u3001Protein\u65b9\u6cd5\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u306f\u3069\u3046\u306a\u3093\u3067\u3057\u3087\u3046\u3002<br \/>\n\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3082\u6c17\u306b\u306a\u308a\u307e\u3059\u3002<\/p>\n<p>\u305d\u3046\u3001\u4f9b\u4e0e\u65b9\u6cd5\u3092\u9078\u3076\u3068\u304d\u306f\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u306e\u307b\u304b\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3084\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3082\u8003\u3048\u306a\u304f\u3066\u306f\u306a\u308a\u307e\u305b\u3093\u3002\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3067\u8ecd\u914d\u304c\u4e0a\u304c\u308b\u306e\u306fDNA\uff08vector\uff09\u65b9\u6cd5\u3067\u3059\u3002\u9078\u629e\u30de\u30fc\u30ab\u30fc\uff08\u86cd\u5149\u30bf\u30f3\u30d1\u30af\u8cea\u3084\u85ac\u5264\u62b5\u6297\u30bf\u30f3\u30d1\u30af\u8cea\uff09\u304c\u63b2\u8f09\u3055\u308c\u3066\u3044\u308b\u306e\u3067\u3001\u5c0e\u5165\u3055\u308c\u305f\u7d30\u80de\u306e\u6fc3\u7e2e\u3084\u5c0e\u5165\u52b9\u7387\u306e\u78ba\u8a8d\u304c\u3057\u3084\u3059\u3044\u3067\u3059\u3002\u4e00\u65b9\u3001Protein\u65b9\u6cd5\u306fDNA\u65b9\u6cd5\u3068\u6bd4\u3079\u3066\u3001\u4e00\u822c\u7684\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u306f\u9ad8\u304f\u3042\u308a\u307e\u305b\u3093\u3002\u305d\u306e\u305f\u3081\u3001\u57f9\u990a\u7d30\u80de\u306b\u52b9\u7387\u3088\u304fCas9\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u5c0e\u5165\u3067\u304d\u308b\u306e\u304b\u3001\u3042\u3089\u304b\u3058\u3081\u78ba\u8a8d\u304c\u5fc5\u8981\u3067\u3059\u3002<img loading=\"lazy\" decoding=\"async\" class=\"aligncenter size-full wp-image-13983\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-03-e1682407330444.jpg\" alt=\"\" width=\"389\" height=\"260\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-03-e1682407330444.jpg 389w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-03-e1682407330444-300x201.jpg 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-03-e1682407330444-96x65.jpg 96w\" sizes=\"auto, (max-width: 389px) 100vw, 389px\" \/><\/p>\n<p>\u307e\u305f\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u306fProtein\u65b9\u6cd5\u304c\u6700\u3082\u4f4e\u3044\u3067\u3059\u3002\u3053\u308c\u306f\u3001\u30bf\u30f3\u30d1\u30af\u8cea\u304c\u7d30\u80de\u5185\u306b\u6b8b\u308a\u3065\u3089\u304f\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u4ee5\u5916\u306e\u907a\u4f1d\u5b50\u306b\u4f5c\u7528\u3057\u3065\u3089\u3044\u305f\u3081\u3060\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n3\u3064\u306e\u65b9\u6cd5\u3092\u307e\u3068\u3081\u3066\u307f\u305f\u3088<\/p>\n<ul>\n<li>\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u306e\u9ad8\u3055\uff1aProtein\uff1eRNA\uff1eDNA(Vector)<\/li>\n<li>\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u306e\u4f4e\u3055\uff1aProtein\uff1eRNA\uff1eDNA(Vector)<\/li>\n<li>\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u306e\u9ad8\u3055<sup>\u203b<\/sup>\uff1aDNA(Vector)\uff1eRNA\uff1eProtein<br \/>\n<span style=\"font-size: small\">\u203b\u6bd4\u8f03\u304c\u96e3\u3057\u304f\u3001\u76f4\u63a5\u6bd4\u8f03\u3057\u305f\u30c7\u30fc\u30bf\u304c\u306a\u3044\u305f\u3081\u3001\u539f\u7406\u304b\u3089\u63a8\u6e2c\u3055\u308c\u308b\u9806\u756a\u3067\u3059\u3002<\/span><\/li>\n<\/ul>\n<p><span style=\"color: #3366ff\"><img loading=\"lazy\" decoding=\"async\" class=\"aligncenter wp-image-14114 size-full\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-04-e1683527966161.png\" alt=\"\" width=\"3370\" height=\"1823\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-04-e1683527966161.png 3370w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-04-e1683527966161-300x162.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-04-e1683527966161-1024x554.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-04-e1683527966161-768x415.png 768w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-04-e1683527966161-1536x831.png 1536w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/04\/gene-editing_bid_ts_3-04-e1683527966161-2048x1108.png 2048w\" sizes=\"auto, (max-width: 3370px) 100vw, 3370px\" \/>A\u3055\u3093<\/span>\uff1a<br \/>\n\u3058\u3083\u3042\u3001Protein\u65b9\u6cd5\u3067\u8a66\u3057\u3066\u307f\u307e\u3059\uff01<br \/>\n\u3048\u3063\u3068\u3001\u5b9f\u9a13\u306b\u5fc5\u8981\u306a\u306e\u306f\u2026<\/p>\n<p><span style=\"color: #ff00ff\">\u5148\u8f29<\/span>\uff1a<br \/>\n\u307e\u305a\u306f\u5927\u4e8b\u306a2\u3064\u3002<br \/>\n\u30ac\u30a4\u30c9RNA\u3068Cas9 Protein\u3060\u306d\u3002<\/p>\n<p>\u4f9b\u4e0e\u65b9\u6cd5\u304c\u6c7a\u307e\u3063\u305f\u3089\u3001\u88681\u3092\u53c2\u8003\u306b\u3057\u3066Cas9\u3068\u30ac\u30a4\u30c9RNA\u3092\u7528\u610f\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n<table border=\"2\" cellpadding=\"10%\">\n<caption>\u88681\u3000Cas9\u3068\u30ac\u30a4\u30c9RNA\u306e\u6e96\u5099\u65b9\u6cd5<\/caption>\n<thead>\n<tr bgcolor=\"aliceblue\">\n<td style=\"text-align: center\" width=\"75\"><\/td>\n<td style=\"text-align: center\" width=\"104\"><b>Cas9<\/b><\/td>\n<td style=\"text-align: center\" width=\"161\"><b>\u30ac\u30a4\u30c9RNA<\/b><\/td>\n<td style=\"text-align: center\" width=\"226\"><b>\u5099\u8003<\/b><\/td>\n<\/tr>\n<\/thead>\n<tbody>\n<tr>\n<td width=\"75\">DNA\u65b9\u6cd5<br \/>\n(Vector)<\/td>\n<td width=\"104\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A21174?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">Invitrogen\u2122 GeneArt\u2122 CRISPR vector<\/a>\u30b7\u30b9\u30c6\u30e0<\/td>\n<td width=\"161\">Invitrogen\u2122 TrueDesign Genome Editor\u306a\u3069\u3067\u914d\u5217\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u3001Primer\u5408\u6210\u306a\u3069\u3067\u5165\u624b<sup>\u203b<\/sup><\/td>\n<td width=\"226\">CRISPR vector\u306f\u54fa\u4e73\u985e\u7d30\u80de\u7528\u306evector\u306e\u305f\u3081\u3001\u54fa\u4e73\u985e\u7d30\u80de\u4ee5\u5916\u306e\u30b5\u30f3\u30d7\u30eb\u306e\u5834\u5408\u306f\u3001\u305d\u306e\u7d30\u80de\u7a2e\u306b\u5bfe\u5fdc\u3057\u305fvector\u3092\u3054\u7528\u610f\u304f\u3060\u3055\u3044\u3002<\/td>\n<\/tr>\n<tr>\n<td width=\"75\">RNA\u65b9\u6cd5<\/td>\n<td width=\"104\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A29378?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">Invitrogen\u2122 GeneArt\u2122 Cas9 mRNA<\/a><\/td>\n<td rowspan=\"2\" width=\"161\"><a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/genome-editing\/crispr-libraries\/trueguide-grnas.html?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">Invitrogen\u2122 TrueGuide RNA<\/a>\uff08\u30c7\u30b6\u30a4\u30f3\u30b5\u30a4\u30c8\uff09\u3067SpCas9\u7528\u30ac\u30a4\u30c9RNA\u304c\u5165\u624b\u53ef\u80fd<\/td>\n<td width=\"226\">Cas9 mRNA\u306fSpCas9 mRNA\u3067\u3001\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u304c\u4ed8\u4e0e<\/td>\n<\/tr>\n<tr>\n<td width=\"75\">Protein\u65b9\u6cd5<\/td>\n<td width=\"104\"><a href=\"https:\/\/www.thermofisher.com\/order\/catalog\/product\/A36496?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">TrueCut Cas9 Protein<\/a><\/td>\n<td width=\"226\">Invitrogen\u2122 TrueCut Cas9 Protein\u306fSpCas9 Protein\u3067\u3001\u6838\u79fb\u884c\u30b7\u30b0\u30ca\u30eb\u304c\u4ed8\u4e0e<\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n<p><span style=\"font-size: small\">\u203b3\u2019\u672b\u7aef\u5074\u306b\u914d\u5217\u306e\u4ed8\u52a0\u304c\u5fc5\u8981\u306a\u3069\u3001\u3044\u304f\u3064\u304b\u6ce8\u610f\u70b9\u304c\u3042\u308a\u307e\u3059\u3002\u30ac\u30a4\u30c9RNA\u3092\u5408\u6210\u3059\u308b\u524d\u306b\u3001\u5fc5\u305aCRISPR vector\u306e\u30d7\u30ed\u30c8\u30b3\u30eb\u304b\u3089\u8a73\u7d30\u3092\u78ba\u8a8d\u3057\u3066\u304f\u3060\u3055\u3044\u3002<\/span><\/p>\n<\/div>\n<p>A\u3055\u3093\u306f\u88681\u3092\u53c2\u8003\u306b\u3057\u3066\u3001Cas9 Protein\u3068\u30ac\u30a4\u30c9RNA\u3092\u624b\u306b\u5165\u308c\u308b\u3053\u3068\u306b\u3057\u307e\u3057\u305f\u3002\u6b21\u56de\u306f\u7528\u610f\u3057\u305fCas9\u3068\u30ac\u30a4\u30c9RNA\u3092\u7528\u3044\u3066\u3001A\u3055\u3093\u304c\u3044\u3088\u3044\u3088\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u6311\u307f\u307e\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<ol>\n<li>CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306b\u306f3\u3064\u306e\u4f9b\u4e0e\u65b9\u6cd5\uff08DNA(Vector)\u3001RNA\u3001\u304a\u3088\u3073Protein\u65b9\u6cd5)\u304c\u3042\u308b<\/li>\n<li>Protein\u65b9\u6cd5\u304c\u30b2\u30ce\u30e0\u7de8\u96c6\u52b9\u7387\u304c\u9ad8\u304f\u3001\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\u3082\u4f4e\u3044<\/li>\n<\/ol>\n<p>A\u3055\u3093\u304c\u521d\u3081\u3066\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u306b\u6311\u6226\u3059\u308b\u30b7\u30ea\u30fc\u30ba<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_4\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" rel=\"noopener\" target=\"_blank\">\u7b2c4\u56de<\/a>\u3067\u306f\u3001\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306e\u30dd\u30a4\u30f3\u30c8\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n<p>\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u30b7\u30ea\u30fc\u30ba\uff1e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_1\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c1\u56de\u3000CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306e\u539f\u7406\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_2\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c2\u56de\u3000\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\uff5e<\/a><br \/>\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c3\u56de\u30003\u3064\u306eCRIPSR\/Cas9\u5b9f\u9a13\u7cfb\uff5e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_4\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c4\u56de\u3000\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_5\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c5\u56de\u3000\u5909\u7570\u306e\u78ba\u8a8d\u65b9\u6cd5\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_6\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c6\u56de\u3000\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\uff5e<\/a><br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_7\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c7\u56de\u3000\u30ce\u30c3\u30af\u30a4\u30f3\uff5e<\/a><\/p>\n<p>\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u57fa\u790e\u7684\u306a\u5185\u5bb9\uff1e<br \/>\n<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/crispr-cas9_basic_bid_ts_1\/?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u539f\u7406\u3068\u624b\u5f15\u304d<\/a><\/p>\n<h3>CRISPR\/Cas9 \u30b9\u30bf\u30fc\u30bf\u30fc\u30d1\u30c3\u30af\u3067\u5b9f\u969b\u306b\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u8a66\u3057\u3066\u307f\u3088\u3046\uff01\uff01<\/h3>\n<p><a class=\"btn btn-red\" href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/products-and-services\/services\/technical-services\/starter-pack.html?CID=bid_syn_gns_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_lgn_kt_s00_gene_editing_bid_ts_3_Social_LAB\" target=\"_blank\" rel=\"noopener noreferrer\">\u8a73\u3057\u304f\u306f\u3053\u3061\u3089<\/a><\/p>\n<p>&nbsp;<\/p>\n<p>\u7814\u7a76\u7528\u306b\u306e\u307f\u4f7f\u7528\u3067\u304d\u307e\u3059\u3002\u8a3a\u65ad\u7528\u306b\u306f\u4f7f\u7528\u3044\u305f\u3060\u3051\u307e\u305b\u3093\u3002<\/p>\n","protected":false},"excerpt":{"rendered":"<p>\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u521d\u5fc3\u8005A\u3055\u3093\u306b\u3088\u308b\u300c\u521d\u3081\u3066\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u300d\u3002\u7b2c1\u56de\u3001\u7b2c2\u56de\u3092\u901a\u3057\u3066\u57fa\u790e\u7684\u306a\u5185\u5bb9\u3092\u7406\u89e3\u3057\u305fA\u3055\u3093\u306f\u3001\u3044\u3088\u3044\u3088\u5b9f\u9a13\u3092\u59cb\u3081\u3088\u3046\u3068\u3057\u3066\u3044\u307e\u3059\u3002 A\u3055\u3093\uff1a \u30ac\u30a4\u30c9RNA\u304c\u30c7\u30b6\u30a4\u30f3\u3067\u304d\u307e\u3057\u305f\uff01 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