{"id":14035,"date":"2023-05-09T17:17:36","date_gmt":"2023-05-09T08:17:36","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=14035"},"modified":"2026-06-29T16:17:07","modified_gmt":"2026-06-29T07:17:07","slug":"cloning-bid-ts-1","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/cloning-bid-ts-1\/","title":{"rendered":"\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\uff5e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30fb\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u3067\u8ff7\u5b50\u306b\u306a\u3089\u306a\u3044\u305f\u3081\u306b\u3000 \u7b2c1\u56de \u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306e\u69cb\u9020\u3068\u30de\u30c3\u30d7\u304b\u3089\u5206\u304b\u308b\u3053\u3068"},"content":{"rendered":"\n<div class=\"wp-block-group is-nowrap is-layout-flex wp-container-core-group-is-layout-ad2f72ca wp-block-group-is-layout-flex\">\n<p>\u66f4\u65b0\u65e5 :<\/p>\n\n\n<div class=\"wp-block-post-date__modified-date wp-block-post-date\"><time datetime=\"2026-06-29T16:17:07+09:00\">6.29.2026<\/time><\/div><\/div>\n\n\n\n<div class=\"wp-block-tf-blocks-intro-summary\">\n<p>\u3053\u306e\u8a18\u4e8b\u306e\u8981\u70b9<\/p>\n\n\n\n<ul 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href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-cloning-2-bid-ts-mbr-24076\/?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cloning_bid_ts_1_Social_LAB\">\u7b2c2\u56de \u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306b\u542b\u307e\u308c\u308b\u30a8\u30ec\u30e1\u30f3\u30c8\u30ea\u30b9\u30c8<\/a><br>\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306b\u542b\u307e\u308c\u308b\u30a8\u30ec\u30e1\u30f3\u30c8\u306e\u5f79\u5272\u3001\u6a5f\u80fd\u306a\u3069\u3092\u307e\u3068\u3081\u305f\u30ea\u30b9\u30c8\u3067\u3059\u3002\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u3092\u8aad\u307f\u89e3\u304f\u969b\u306b\u8f9e\u66f8\u4ee3\u308f\u308a\u306b\u3054\u5229\u7528\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-cloning-3-bid-ts-mbr-25003\/?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cloning_bid_ts_1_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">\u7b2c3\u56de \u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\uff1a\u5b9f\u4f8b\u96c61<\/a><br><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-cloning-bid-ts-mbr-25035\/?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cloning_bid_ts_1_Social_LAB\" target=\"_blank\" rel=\"noreferrer noopener\">\u7b2c4\u56de \u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306e\u8aad\u307f\u65b9\uff1a\u5b9f\u4f8b\u96c62<\/a><br>\u5fdc\u7528\u7de8\u3067\u3059\u3002\u5b9f\u969b\u306b\u30d9\u30af\u30bf\u30fc\u3092\u5165\u624b\u3057\u305f\u969b\u306b\u3069\u306e\u3088\u3046\u306b\u6271\u3048\u3070\u3088\u3044\u306e\u304b\u3092\u51b7\u9759\u306b\u898b\u6975\u3081\u308b\u305f\u3081\u306e\u624b\u9806\u3001\u8003\u3048\u65b9\u306b\u3064\u3044\u3066\u3001\u5b9f\u4f8b\u3092\u4f7f\u3063\u3066\u3054\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u3068\u306f\">\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u3068\u306f\uff1f<\/h2>\n\n\n\n<p>\u305d\u3082\u305d\u3082\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u3068\u306f\u3069\u3093\u306a\u3082\u306e\u3067\u3057\u3087\u3046\u304b\uff1f<br>\u30d7\u30e9\u30b9\u30df\u30c9\u306f\u5927\u8178\u83cc\u306a\u3069\u30d0\u30af\u30c6\u30ea\u30a2\u304c\u30b2\u30ce\u30e0\u5916\u306b\u6301\u3064\u5c0f\u3055\u306a\u74b0\u72b62\u672c\u9396DNA\u3067\u3001\u83cc\u5185\u3067\u81ea\u5df1\u8907\u88fd\u3067\u304d\u307e\u3059\u3002\u4e0d\u8981\u306a\u90e8\u5206\u3092\u9664\u304d\u3001\u5fc5\u8981\u306a\u30a8\u30ec\u30e1\u30f3\u30c8\u3092\u633f\u5165\u3059\u308b\u306a\u3069\u3001\u4f7f\u3044\u6613\u3044\u3088\u3046\u306b\u6539\u5909\u3057\u305f\u3082\u306e\u304c\u5b9f\u9a13\u306b\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u4e00\u65b9\u3001\u30d9\u30af\u30bf\u30fc\u306e\u8aac\u660e\u3068\u3057\u3066\u306f\u300c\u904b\u3073\u5c4b\u300d\u3068\u3044\u3046\u8a00\u8449\u304c\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\u3002\u3064\u307e\u308a\u3001\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u3068\u306f\u3001\u907a\u4f1d\u5b50\u306a\u3069\u3092\u904b\u3076\u9053\u5177\u3068\u3057\u3066\u6539\u5909\u3055\u308c\u305f\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u3053\u3068\u3092\u6307\u3057\u307e\u3059\uff08\u56f31\uff09\u3002\u4ed6\u306b\u30a6\u30a4\u30eb\u30b9\u306b\u5916\u6765\u907a\u4f1d\u5b50\u306a\u3069\u3092\u7d44\u307f\u8fbc\u3093\u3060\u30a6\u30a4\u30eb\u30b9\u30d9\u30af\u30bf\u30fc\u3068\u3044\u3046\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u306e\u3067\u3054\u6ce8\u610f\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full wp-image-14036\"><img loading=\"lazy\" decoding=\"async\" width=\"634\" height=\"318\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5.png\" alt=\"\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\" class=\"wp-image-14036\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5.png 634w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/29d3bc99193cb0b837fb8cc53f050ae5-300x150.png 300w\" sizes=\"auto, (max-width: 634px) 100vw, 634px\" \/><figcaption class=\"wp-element-caption\"><strong>\u56f31. \u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306f\u907a\u4f1d\u5b50\u306a\u3069\u3092\u904b\u3076\u305f\u3081\u306e\u9053\u5177\u3067\u3001\u5927\u8178\u83cc\u5185\u3067\u8907\u88fd\u3067\u304d\u308b<\/strong><\/figcaption><\/figure>\n\n\n\n<p>\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306f\u3001\u305d\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306b\u3069\u306e\u3088\u3046\u306a\u9053\u5177\u30fb\u8981\u7d20\uff08\u30a8\u30ec\u30e1\u30f3\u30c8\uff09\u304c\u542b\u307e\u308c\u308b\u304b\u3092\u56f3\u793a\u3057\u305f\u3082\u306e\u3067\u3059\u3002\u30de\u30c3\u30d7\u304b\u3089\u6271\u3044\u65b9\u3001\u3059\u306a\u308f\u3061\u4f55\u306b\u3001\u3069\u306e\u3088\u3046\u306b\u4f7f\u3048\u308b\u304b\u3092\u8aad\u307f\u53d6\u308c\u307e\u3059\u3002\u305f\u3060\u3057\u3001\u591a\u304f\u306e\u77e5\u3089\u306a\u3044\u30a8\u30ec\u30e1\u30f3\u30c8\u540d\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u3066\u3001\u4f7f\u3046\u524d\u306b\u3069\u3053\u3092\u898b\u305f\u3089\u3088\u3044\u306e\u304b\u5206\u304b\u308a\u306b\u304f\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u5b9f\u969b\u306f\u5168\u3066\u3092\u5b8c\u5168\u306b\u7406\u89e3\u3059\u308b\u5fc5\u7528\u306f\u3042\u308a\u307e\u305b\u3093\u304c\u3001\u4e00\u3064\u3067\u3082\u5206\u304b\u3089\u306a\u3044\u30a8\u30ec\u30e1\u30f3\u30c8\u304c\u3042\u308b\u3068\u4e0d\u5b89\u3001\u3068\u3044\u3046\u3053\u3068\u3082\u3042\u308b\u3068\u601d\u3044\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u672c\u30d6\u30ed\u30b0\u3067\u306f\u3001\u77e5\u3063\u3066\u304a\u304f\u5fc5\u8981\u304c\u3042\u308b\u4e3b\u8981\u30a8\u30ec\u30e1\u30f3\u30c8\u306b\u3064\u3044\u3066\u3001InvitrogenTM pcDNATM3.1(+) Mammalian Expression Vector\uff08\u56f32\uff09\u3092\u4f8b\u306b\u3057\u3001\u4ee5\u4e0b\u306e\u9806\u3067\u7c21\u5358\u306b\u8aac\u660e\u3057\u307e\u3059\u3002<\/p>\n\n\n\n<ol class=\"wp-block-list\">\n<li>\u5fc5\u9808\u30a8\u30ec\u30e1\u30f3\u30c83\u7a2e\uff08origin\u3001\u6297\u751f\u7269\u8cea\u8010\u6027\u907a\u4f1d\u5b50\u3001\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\uff09<\/li>\n\n\n\n<li>\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u5fc5\u8981\u306a\u8ee2\u5199\u3001\u7ffb\u8a33\u95a2\u9023\u30a8\u30ec\u30e1\u30f3\u30c8<\/li>\n\n\n\n<li>\u305d\u306e\u4ed6\u306e\u3088\u304f\u898b\u304b\u3051\u308b\u30a8\u30ec\u30e1\u30f3\u30c8<\/li>\n<\/ol>\n\n\n\n<p>\u3053\u306e\u30d6\u30ed\u30b0\u3092\u3054\u89a7\u3044\u305f\u3060\u3051\u308c\u3070\u3001\u591a\u304f\u306e\u4e00\u822c\u7684\u306a\u30de\u30c3\u30d7\u306f\u3060\u3044\u305f\u3044\u300c\u8aad\u3081\u308b\u300d\u3088\u3046\u306b\u306a\u308b\u3068\u601d\u3044\u307e\u3059\u304c\u3001\u5206\u304b\u3089\u306a\u3044\u30a8\u30ec\u30e1\u30f3\u30c8\u304c\u3042\u3063\u305f\u3089\u4e0d\u5b89\u3068\u3044\u3046\u65b9\u306f\u3001\u7b2c2\u56de\u306e\u30a8\u30ec\u30e1\u30f3\u30c8\u30ea\u30b9\u30c8\u3092\u4e00\u7dd2\u306b\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-1-3\u7a2e\u306e\u5fc5\u9808\u30a8\u30ec\u30e1\u30f3\u30c8\">1. 3\u7a2e\u306e\u5fc5\u9808\u30a8\u30ec\u30e1\u30f3\u30c8<\/h2>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full wp-image-14044\"><img loading=\"lazy\" decoding=\"async\" width=\"281\" height=\"337\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/cbf5b9f60da99bf791c0aa12a770f429.png\" alt=\"pcDNA3.1\u306e\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\uff1a\u5fc5\u9808\u30a8\u30ec\u30e1\u30f3\u30c8 \" class=\"wp-image-14044\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/cbf5b9f60da99bf791c0aa12a770f429.png 281w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/cbf5b9f60da99bf791c0aa12a770f429-250x300.png 250w\" sizes=\"auto, (max-width: 281px) 100vw, 281px\" \/><figcaption class=\"wp-element-caption\"><strong>\u56f32. pcDNA3.1\u306e\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\uff1a<br>\u5fc5\u9808\u30a8\u30ec\u30e1\u30f3\u30c8<\/strong><\/figcaption><\/figure>\n\n\n\n<p><u>origin\uff1a\u56f32\u306epUC ori<\/u><br>\u3008\u6ce8\u610f\u3009f1 ori\u3067\u306f\u306a\u3044\uff08\u30d6\u30ed\u30b0\u7b2c2\u56de\u53c2\u7167\uff09\u3002SV40 ori\u3067\u3082\u306a\u3044\uff08\u5f8c\u8ff0\uff09\u3002<br>\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u5fc5\u9808\u6a5f\u80fd\u3067\u3042\u308b\u81ea\u5df1\u8907\u88fd\u80fd\u529b\u306b\u95a2\u308f\u308b\u30a8\u30ec\u30e1\u30f3\u30c8\u3067\u3001\u3053\u3053\u304b\u3089\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u8907\u88fd\u304c\u958b\u59cb\u3055\u308c\u307e\u3059\u3002\u5fc5\u9808\u30a8\u30ec\u30e1\u30f3\u30c8\u3067\u3059\u304c\u3001\u591a\u304f\u306e\u5834\u5408\u300c\u3053\u3053\u306borigin\u304c\u3042\u308b\u306a\u300d\u3068\u77e5\u3063\u3066\u304a\u3044\u3066\u3044\u305f\u3060\u304f\u3060\u3051\u3067\u5341\u5206\u3067\u3059\u3002<br><b>\u6a5f\u80fd\u30fb\u5f79\u5272\uff1a<\/b>\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u5927\u8178\u83cc\u5185\u3067\u81ea\u5df1\u8907\u88fd\u3059\u308b\u969b\u306e\u8d77\u70b9\u3068\u306a\u308b\u30a8\u30ec\u30e1\u30f3\u30c8<br><b>\u30de\u30c3\u30d7\u4e0a\u306e\u8a18\u8f09\uff1a<\/b>origin\uff08\u30aa\u30ea\u30b8\u30f3\uff09\u3001ori\uff08\u30aa\u30ea\uff09\u3001\u3042\u308b\u3044\u306f\u7a2e\u985e\u540d\uff08pUC\u306a\u3069\uff09<br><b>\u7a2e\u985e\uff1a<\/b>pUC\u3001pBR322\u3001ColE1\u3001pMB\u306a\u3069<\/p>\n\n\n\n<div style=\"background: #F7F7F7;border: 1px solid #A1A1A1;padding: 10px\">\uff5e\u30e1\u30e21\uff5e<br>\u30d7\u30e9\u30b9\u30df\u30c9\u306e\u30b3\u30d4\u30fc\u6570\u306b\u3064\u3044\u3066<br>origin\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306e\u30b3\u30d4\u30fc\u6570\uff081\u83cc\u4f53\u3042\u305f\u308a\u306e\u500b\u6570\uff09\u304c\u7570\u306a\u308a\u307e\u3059\u3002\u30b3\u30d4\u30fc\u6570\u306b\u95a2\u3057\u3066\u306f\u3001\u4ee5\u4e0b3\u70b9\u3092\u6c17\u306b\u7559\u3081\u3066\u304a\u3044\u3066\u3044\u305f\u3060\u3051\u308c\u3070\u5341\u5206\u3067\u3059\u3002\n<ul>\n<li>\u30cf\u30a4\u30b3\u30d4\u30fc\u30bf\u30a4\u30d7\uff08pUC\u306a\u3069\u3001\u6570\u767e\u500b\/\u83cc\u4f53\uff09\u3001\u3068\u30ed\u30fc\u30b3\u30d4\u30fc\u30bf\u30a4\u30d7\uff08ColE1\u3001pBR322\u3001pMB1\u306a\u3069\u3001\u6570\u5341\u500b\/\u83cc\u4f53\uff09<br>\u203b\u305f\u3060\u3057\u3001ColE1\u3068\u66f8\u3044\u3066\u3042\u3063\u3066\u3082\u305d\u306e\u6539\u826f\u578b\u306epUC\u306e\u53ef\u80fd\u6027\u3082\u3042\u308b\u306e\u3067\u3001\u3042\u307e\u308a\u6c17\u306b\u3057\u3066\u3082\u4ed5\u65b9\u304c\u7121\u3044\u304b\u3082\u3057\u308c\u307e\u305b\u3093\u3002\u7279\u306b\u7406\u7531\u304c\u7121\u3051\u308c\u3070\u591a\u304f\u306e\u5834\u5408\u30cf\u30a4\u30b3\u30d4\u30fc\u30bf\u30a4\u30d7\u304c\u4f7f\u308f\u308c\u3066\u3044\u307e\u3059\u3002<\/li>\n<li>\u5927\u8178\u83cc\u304c\u5acc\u3046\u914d\u5217\uff08\u7e70\u308a\u8fd4\u3057\u914d\u5217\u3001\u6bd2\u6027\u907a\u4f1d\u5b50\u914d\u5217\u306a\u3069\uff09\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3059\u308b\u5834\u5408\u3001\u304a\u3088\u3073\u5927\u304d\u3081\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306e\u5834\u5408\u306f\u30ed\u30fc\u30b3\u30d4\u30fc\u30bf\u30a4\u30d7\u306eorigin\u306e\u65b9\u304c\u5411\u3044\u3066\u3044\u308b\u3068\u3044\u308f\u308c\u3066\u3044\u307e\u3059\u3002\u307e\u305f\u3001\u5927\u8178\u83cc\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc\u3067\u3042\u308bpET\u7cfb\u30d9\u30af\u30bf\u30fc\u3067\u306fpBR322\u304c\u4f7f\u308f\u308c\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002\u306a\u304a\u3001pUC\u306e\u5834\u5408\u3001\u57f9\u990a\u6e29\u5ea6\u309225\uff5e30 \u2103\u306b\u4e0b\u3052\u308b\u3068\u30b3\u30d4\u30fc\u6570\u3092\u4e0b\u3052\u3089\u308c\u308b\u306e\u3067\u305d\u308c\u3067\u5bfe\u5fdc\u3067\u304d\u308b\u30b1\u30fc\u30b9\u3082\u591a\u305d\u3046\u3067\u3059\u3002<\/li>\n<li>\u5f53\u7136\u306a\u304c\u3089\u30ed\u30fc\u30b3\u30d4\u30fc\u30bf\u30a4\u30d7\u306f\u30d7\u30e9\u30b9\u30df\u30c9\u62bd\u51fa\u3067\u9ad8\u53ce\u91cf\u3092\u671b\u3081\u307e\u305b\u3093\u3002\u305f\u3060\u3057\u3001\u3053\u308c\u3082\u30af\u30ed\u30e9\u30e0\u30d5\u30a7\u30cb\u30b3\u30fc\u30eb\u3092\u6dfb\u52a0\u3057\u3066\u4e00\u6642\u7684\u306b\u30b3\u30d4\u30fc\u6570\u3092\u5897\u3084\u3059\u65b9\u6cd5\u3067\u3042\u308b\u7a0b\u5ea6\u5bfe\u51e6\u53ef\u80fd\u3067\u3059\u3002<\/li>\n<\/ul>\n<\/div>\n\n\n\n<p><u><br>\u6297\u751f\u7269\u8cea\u8010\u6027\u907a\u4f1d\u5b50\uff1a\u56f32\u306eAmpicillin\u3068Neomycin<\/u><br>\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u3092\u5c0e\u5165\u3057\u305f\u5927\u8178\u83cc\u306e\u9078\u5225\u30fb\u57f9\u990a\u3092\u884c\u3046\u969b\u306b\u4f7f\u7528\u3067\u304d\u308b\u6297\u751f\u7269\u8cea\u3092\u77e5\u308b\u305f\u3081\u306b\u5fc5\u9808\u306e\u60c5\u5831\u3067\u3059\u3002\u30de\u30c3\u30d7\u306b\u6297\u751f\u7269\u8cea\u540d\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u306f\u305a\u306a\u306e\u3067\u3001\u306a\u3093\u3068\u306a\u304f\u3053\u308c\u304b\u306a\u3068\u5206\u304b\u308b\u306e\u3067\u901a\u5e38\u554f\u984c\u7121\u3044\u3067\u3059\u304c\u30012\u3064\u4ee5\u4e0a\u898b\u3064\u304b\u3063\u305f\u5834\u5408\u8981\u6ce8\u610f\u3067\u3059\u3002\u4e0a\u8a18\u306e\u4f8b\u3067\u306f\u3001Ampicillin\u304c\u5927\u8178\u83cc\u7528\u3001Neomycin\u304c\u57f9\u990a\u7d30\u80de\uff08\u5bbf\u4e3b\uff09\u7528\u3067\u3059\u3002\u898b\u5206\u3051\u65b9\u306f\u5e7e\u3064\u304b\u3042\u308a\u307e\u3059\u304c\u3001\u5bbf\u4e3b\u7528\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08SV40 ori\u3001\u5206\u304b\u308a\u306b\u304f\u3044\u3067\u3059\u304cSV40\u30a6\u30a4\u30eb\u30b9\u306eorigin\u3092\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u3057\u3066\u4f7f\u7528\u3057\u3066\u3044\u307e\u3059\uff09\u304c\u3042\u308b\u304b\u3069\u3046\u304b\u3067\u898b\u5206\u3051\u308b\u306e\u304c\u3088\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u5bbf\u4e3b\u306b\u95a2\u3057\u3066\u306f\u30d6\u30ed\u30b0\u7b2c2\u56de\u306b\u307e\u3068\u3081\u307e\u3059\u306e\u3067\u3088\u308d\u3057\u3051\u308c\u3070\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<br><b>\u6a5f\u80fd\u30fb\u5f79\u5272\uff1a<\/b>\u30d7\u30e9\u30b9\u30df\u30c9\u304c\u5c0e\u5165\u3055\u308c\u305f\u7d44\u307f\u63db\u3048\u751f\u7269\u30fb\u57f9\u990a\u7d30\u80de\u7b49\u5bbf\u4e3b\u3092\u9078\u5225\u3059\u308b\u305f\u3081\u306b\u4f7f\u7528<br><b>\u30de\u30c3\u30d7\u4e0a\u306e\u8a18\u8f09\uff1a<\/b>Ampicillin\u30fbAmp\u306a\u3069\u6297\u751f\u7269\u8cea\u540d\u3001\u3042\u308b\u3044\u306fAmpicillinR\u30fbAmpR\u306e\u3088\u3046\u306b\u6297\u751f\u7269\u8cea\u540d\u306e\u5f8c\u308d\u306bR\uff08resistance gene\u306e\u7565\uff09\u3092\u4ed8\u3051\u305f\u3082\u306e\u304c\u591a\u3044<br><b>\u7a2e\u985e\uff1a<\/b> ampicillin\u8010\u6027\u907a\u4f1d\u5b50\u3001kanamycin\u8010\u6027\u907a\u4f1d\u5b50\uff08neomycin\u8010\u6027\u907a\u4f1d\u5b50\u3001\u57fa\u672c\u540c\u3058\u3082\u306e\uff09\u306a\u3069\u3001\u3088\u304f\u4f7f\u308f\u308c\u308b\u3082\u306e\u304c10\u7a2e\u7a0b\u5ea6<\/p>\n\n\n\n<div style=\"background: #F7F7F7;border: 1px solid #A1A1A1;padding: 10px\">\uff5e\u30e1\u30e22\uff5e<br>\u6297\u751f\u7269\u8cea\u306b\u306f\u539f\u6838\u751f\u7269\u306e\u307f\u306b\u52b9\u304f\u3082\u306e\u3001\u539f\u6838\u751f\u7269\u3068\u771f\u6838\u751f\u7269\u4e21\u65b9\u306b\u52b9\u304f\u3082\u306e\u304c\u3042\u308a\u307e\u3059\u3002<br>\u539f\u6838\u751f\u7269\u306e\u307f\uff1aampicillin\u3001kanamycin\u3001chloramphenicol\u3001spectinomycin\u3001neomycin\u306a\u3069<br>\u539f\u6838\u751f\u7269\u53ca\u3073\u771f\u6838\u751f\u7269\uff1aG418\uff08= Geneticin) \u3001zeocin\u3001blasticidin\u3001puromycin\u3001hygromycin\u306a\u3069<br>\u306a\u304a\u3001ampicillin\u306f\u539f\u6838\u751f\u7269\u306b\u3057\u304b\u52b9\u304b\u306a\u3044\u306e\u3067\u3001ampicillin\u8010\u6027\u907a\u4f1d\u5b50\u304c\u3042\u308c\u3070\u305d\u308c\u306f\u5927\u8178\u83cc\u7528\u3067\u3001\u5927\u8178\u83cc\u306e\u9078\u5225\u7528\u306b\u306fampicillin\u304c\u4f7f\u7528\u3067\u304d\u308b\u3068\u5224\u65ad\u3067\u304d\u307e\u3059\u3002<\/div>\n\n\n\n<p><u><br>\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\uff1a\u56f32\u306eMCS<\/u><br>\u76ee\u7684\u306eDNA\u914d\u5217\uff08\u907a\u4f1d\u5b50\u306a\u3069\uff09\u3092\u5165\u308c\u308b\u5834\u6240\u3067\u3059\u3002\u56f32\u306e\u3088\u3046\u306b\u591a\u304f\u306e\u5236\u9650\u9175\u7d20\u30b5\u30a4\u30c8\u3092\u4e26\u3079\u305fMCS\uff08\u30de\u30eb\u30c1\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\uff09\u304c\u3042\u3063\u305f\u308a\u3001\u3042\u3089\u304b\u3058\u3081\u8f2a\u304c\u5207\u308c\u305f\u56f3\u306b\u306a\u3063\u3066\u3044\u305f\u308a\u3059\u308b\u306e\u3067\u5224\u308a\u3084\u3059\u3044\u3068\u601d\u3044\u307e\u3059\u3002MCS\u304c\u3042\u308c\u3070\u3001\u305d\u3053\u306b\u3042\u308b\u5236\u9650\u9175\u7d20\u3067\u5207\u65ad\u3057\u3001\u76ee\u7684DNA\u3092DNA\u30ea\u30ac\u30fc\u30bc\u3001\u3042\u308b\u3044\u306f\u30b7\u30fc\u30e0\u30ec\u30b9\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u6cd5\uff08Gibson\u6cd5\u306a\u3069\uff09\u3067\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3067\u304d\u307e\u3059\u3002\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u3092\u7279\u5b9a\u3067\u304d\u305f\u3089\u3001\u76ee\u7684DNA\u3092\u5165\u308c\u308b\u5834\u6240\u3001\u65b9\u6cd5\u304c\u5206\u304b\u308a\u307e\u3059\u3002<br><b>\u6a5f\u80fd\u30fb\u5f79\u5272\uff1a<\/b>\u76ee\u7684\u306eDNA\u914d\u5217\uff08\u907a\u4f1d\u5b50\u306a\u3069\uff09\u3092\u5165\u308c\u308b\uff08\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3059\u308b\uff09\u5834\u6240<br><b>\u30de\u30c3\u30d7\u4e0a\u306e\u8a18\u8f09\uff1a<\/b>\u7a2e\u985e\u540d\uff08\u5f8c\u8ff0\uff09\u3001\u5236\u9650\u9175\u7d20\u30b5\u30a4\u30c8\u540d\u306a\u3069\uff08\uff5e\u30e1\u30e23\uff5e\u53c2\u7167\uff09<br><b>\u7a2e\u985e\uff1a<\/b>MCS\u3001TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3001TOPO\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3001Gateway\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306a\u3069<\/p>\n\n\n\n<div style=\"background: #F7F7F7;border: 1px solid #A1A1A1;padding: 10px\">\uff5e\u30e1\u30e23\uff5e<br>\u30de\u30c3\u30d7\u4e0a\u306e\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u3068\u4f7f\u7528\u53ef\u80fd\u306a\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\u306e\u4f8b\u3092\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002\n<ul>\n<li>MCS \u4f8b\uff09<img loading=\"lazy\" decoding=\"async\" class=\"alignbottom size-full wp-image-14055\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/5e4896966c9944fcf00890f4502605bf-1.png\" alt=\"MCS \u4f8b\" width=\"267\" height=\"60\"><br>\u57fa\u672c\u7684\u306b\u3053\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u3092\u4e00\u30ab\u6240\u3060\u3051\u3067\u5207\u65ad\u3059\u308b\uff08\u30e6\u30cb\u30fc\u30af\u306a\uff09\u5236\u9650\u9175\u7d20\u540d\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\uff1a\u5236\u9650\u9175\u7d20\u3068DNA\u30ea\u30ac\u30fc\u30bc\u3092\u7528\u3044\u308b\u65b9\u6cd5\u3001\u30b7\u30fc\u30e0\u30ec\u30b9\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u6cd5\uff08Gibson\u6cd5\u7b49\uff09<\/li>\n<li>TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u3000\u4f8b\uff09<img loading=\"lazy\" decoding=\"async\" class=\"alignbottom size-full wp-image-14062\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/1efad1b5aa917a846fa5b3fc11367269-1.png\" alt=\"TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u3000\u4f8b\" width=\"312\" height=\"90\"><br>\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u304c\u5207\u65ad\u3055\u308c\u3066\u3044\u3066\u7dda\u72b6\u306b\u306a\u3063\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u4e213\u2019\u672b\u7aef\u306bdT\uff08\u30c7\u30aa\u30ad\u30b7\u30c1\u30df\u30b8\u30f3\uff09\u304c1\u3064\u7a81\u51fa\uff08\u30aa\u30fc\u30d0\u30fc\u30cf\u30f3\u30b0\uff09\u3057\u305f\u69cb\u9020\u3067\u3059\u3002<br>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\uff1aTA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\uff083\u2019\u672b\u7aef\u306bdA\u3001\u30c7\u30aa\u30ad\u30b7\u30a2\u30c7\u30ce\u30b7\u30f3\u304c1\u3064\u7a81\u51fa\u3057\u305fPCR\u7523\u7269\u3092\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\uff09<\/li>\n<li>TOPO-TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u3000\u4f8b\uff09<img loading=\"lazy\" decoding=\"async\" class=\"alignbottom size-full wp-image-14065\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/7d6fddc4d5f4457e598413974089fe73.png\" alt=\"TOPO-TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\" width=\"139\" height=\"98\"><br>TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u3068\u306e\u9055\u3044\u306f\u3001\u672b\u7aef\u306bDNA\u30ea\u30ac\u30fc\u30bc\u306e\u4ee3\u308f\u308a\u3092\u3059\u308b\u30c8\u30dd\u30a4\u30bd\u30e1\u30e9\u30fc\u30bc\u3068\u3044\u3046\u9175\u7d20\u304c\u4ed8\u52a0\u3055\u308c\u3066\u3044\u308b\u70b9\u3067\u3059\u3002<br>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\uff1aTOPO-TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\uff08TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u306e\u6539\u826f\u7248\u3067\u3001DNA\u30ea\u30ac\u30fc\u30bc\u4e0d\u8981\uff09<\/li>\n<li>TOPO-Blunt\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8 \u4f8b\uff09<img loading=\"lazy\" decoding=\"async\" class=\"aligbottom size-full wp-image-14077\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/7adfed136bf50c6b1f5c23f5919c3a0a.png\" alt=\"TOPO-Blunt\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\" width=\"127\" height=\"98\"><br>TOPO-TA\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3068\u306e\u9055\u3044\u306f3\u2019\u672b\u7aef\u306bdT\u304c\u4ed8\u3044\u3066\u3044\u306a\u3044\u70b9\u3067\u3059\u3002<br>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\uff1aTOPO-Blunt\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\uff08\u5e73\u6ed1\u672b\u7aefPCR\u7523\u7269\u3092\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u53ef\u80fd\uff09<\/li>\n<li>Directional TOPO\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8 \u4f8b\uff09<img loading=\"lazy\" decoding=\"async\" class=\"alignbottom size-full wp-image-14078\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/b12c8b7e401505670006e9aa6ee0e2d0.png\" alt=\"Directional TOPO\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\" width=\"147\" height=\"98\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/b12c8b7e401505670006e9aa6ee0e2d0.png 147w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/b12c8b7e401505670006e9aa6ee0e2d0-96x65.png 96w\" sizes=\"auto, (max-width: 147px) 100vw, 147px\" \/><br>TOPO-Blunt\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3068\u306e\u9055\u3044\u306f\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u306e\u5de6\u5074\u306ebottom\u9396\u306e5\u2019\u672b\u306bGTGG\u3068\u3044\u3046\u914d\u5217\u304c\u7a81\u51fa\u3057\u3066\u3044\u308b\u70b9\u3067\u3059\u3002<br>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\uff1aDirectional TOPO\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\uff08\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u65ad\u7247\u306e\u5411\u304d\u3092\u3042\u308b\u7a0b\u5ea6\u6c7a\u3081\u3089\u308c\u308b\uff09<\/li>\n<li>Gateway\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8 \u4f8b\uff09<img loading=\"lazy\" decoding=\"async\" class=\"aligbottom size-full wp-image-14079\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/695e432aa0b80e914efae12cd0be2a70.png\" alt=\"Gateway\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\" width=\"277\" height=\"60\"><br>Gateway\u7d44\u307f\u63db\u3048\u30b5\u30a4\u30c8\uff08att\u914d\u5217\uff09\u3092\u542b\u3080\u4e0a\u8a18\u306e\u3088\u3046\u306a\u914d\u5217\u3092\u6301\u3061\u307e\u3059\u3002<br>\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\uff1aGateway LR\u53cd\u5fdc\u3002Gateway LR Clonase\u3068\u3044\u3046\u9175\u7d20\u3068\u3001\u3042\u3089\u304b\u3058\u3081\u76ee\u7684\u907a\u4f1d\u5b50\u3092\u7d44\u307f\u8fbc\u3093\u3060\u5c02\u7528\u30d9\u30af\u30bf\u30fc\uff08\u30a8\u30f3\u30c8\u30ea\u30fc\u30af\u30ed\u30fc\u30f3\uff09\u304c\u3042\u308c\u3070\u3001\u30d9\u30af\u30bf\u30fc\u3092\u5207\u65ad\u305b\u305a\u306b\u30c1\u30e5\u30fc\u30d6\u5185\u3067\u7d44\u307f\u63db\u3048\u3092\u884c\u3048\u307e\u3059\u3002<\/li>\n<\/ul>\n<\/div>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-2-\u8ee2\u5199-\u7ffb\u8a33\u95a2\u9023\u30a8\u30ec\u30e1\u30f3\u30c8\">2. \u8ee2\u5199\u3001\u7ffb\u8a33\u95a2\u9023\u30a8\u30ec\u30e1\u30f3\u30c8<\/h2>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full wp-image-14081\"><img loading=\"lazy\" decoding=\"async\" width=\"236\" height=\"296\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/184e1fbbe38c6fb97ef918a7cd7090a8.png\" alt=\"pcDNA3.1\u306e\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\uff1a\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u5fc5\u8981\u306a\u8ee2\u5199\u3001\u7ffb\u8a33\u95a2\u9023\u30a8\u30ec\u30e1\u30f3\u30c8\" class=\"wp-image-14081\" \/><figcaption class=\"wp-element-caption\">\u56f3<strong>3. pcDNA3.1\u306e\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\uff1a\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u5fc5\u8981\u306a\u8ee2\u5199\u3001\u7ffb\u8a33\u95a2\u9023\u30a8\u30ec\u30e1\u30f3\u30c8<\/strong><\/figcaption><\/figure>\n\n\n\n<p>\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u7528\u30d9\u30af\u30bf\u30fc\u306e\u5834\u5408\u3001\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\uff08\u56f33\u306eMCS\uff09\u306e\u4e0a\u6d41\uff08\u56f33\u306e\u2460\uff09\u3001\u4e0b\u6d41\uff08\u56f33\u306e\u2461\uff09\u306b\u3001\u8ee2\u5199\u3001\u7ffb\u8a33\u306b\u95a2\u308f\u308b\u30a8\u30ec\u30e1\u30f3\u30c8\u304c\u633f\u5165\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u305d\u306e\u60c5\u5831\u304b\u3089\u3001\u3069\u306e\u3088\u3046\u306a\u767a\u73fe\u7cfb\u3067\u4f7f\u3048\u308b\u304b\u304c\u5206\u304b\u308a\u307e\u3059\u3002<br>\u4ee5\u4e0b\u306b\u5927\u8178\u83cc\u306a\u3069\u539f\u6838\u751f\u7269\u7528\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3001\u304a\u3088\u3073\u54fa\u4e73\u985e\u7d30\u80de\u306a\u3069\u771f\u6838\u751f\u7269\u7528\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u306b\u3064\u3044\u3066\u3001\u95a2\u9023\u30a8\u30ec\u30e1\u30f3\u30c8\u3092\u307e\u3068\u3081\u3066\u307f\u307e\u3057\u305f\u3002<\/p>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u539f\u6838\u751f\u7269-\u5927\u8178\u83cc\u306a\u3069-\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306b\u5fc5\u8981\u306a\u30a8\u30ec\u30e1\u30f3\u30c8\u4f8b\"><strong>\u539f\u6838\u751f\u7269\uff08\u5927\u8178\u83cc\u306a\u3069\uff09\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306b\u5fc5\u8981\u306a\u30a8\u30ec\u30e1\u30f3\u30c8\u4f8b<\/strong><\/h3>\n\n\n\n<p>\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\uff08\u3053\u3053\u3067\u306f\u76ee\u7684\u907a\u4f1d\u5b50\u306eORF\u304c\u65e2\u306b\u633f\u5165\u3055\u308c\u3066\u3044\u307e\u3059\uff09\u306e\u4e0a\u6d41\u306b\u306f\u8ee2\u5199\u306b\u95a2\u308f\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08Promoter\uff09\u3068\u7ffb\u8a33\u306b\u95a2\u308f\u308bSD\u914d\u5217\uff08\u30ea\u30dc\u30bd\u30fc\u30e0\u7d50\u5408\u30b5\u30a4\u30c8\u3001rbs\uff09\u304c\u3001\u4e0b\u6d41\u306b\u306f\u8ee2\u5199\u306b\u95a2\u308f\u308b\u30bf\u30fc\u30df\u30cd\u30fc\u30bf\u30fc\uff08Term\uff09\u304c\u633f\u5165\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br>\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u4f8b\uff1aT7\u3001Trc\u3001Tac\u3001Lac\u3001araBAD\u306a\u3069<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"566\" height=\"83\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/6d58f877a83b152a3c3b13756107b189.png\" alt=\"\u539f\u6838\u751f\u7269\uff08\u5927\u8178\u83cc\u306a\u3069\uff09\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306b\u5fc5\u8981\u306a\u30a8\u30ec\u30e1\u30f3\u30c8\u4f8b\" class=\"wp-image-14084\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/6d58f877a83b152a3c3b13756107b189.png 566w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/6d58f877a83b152a3c3b13756107b189-300x44.png 300w\" sizes=\"auto, (max-width: 566px) 100vw, 566px\" \/><\/figure>\n\n\n\n<h3 class=\"wp-block-heading\" id=\"h-\u771f\u6838\u751f\u7269-\u54fa\u4e73\u985e\u7d30\u80de\u306a\u3069-\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306b\u5fc5\u8981\u306a\u30a8\u30ec\u30e1\u30f3\u30c8\u4f8b\"><strong>\u771f\u6838\u751f\u7269\uff08\u54fa\u4e73\u985e\u7d30\u80de\u306a\u3069\uff09\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306b\u5fc5\u8981\u306a\u30a8\u30ec\u30e1\u30f3\u30c8\u4f8b<\/strong><\/h3>\n\n\n\n<p>\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u306e\u4e0a\u6d41\u306b\u306f\u8ee2\u5199\u306b\u95a2\u308f\u308b\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\uff08Promoter\uff09\u3068\u7ffb\u8a33\u306b\u95a2\u308f\u308bKozak\u914d\u5217\uff08\u30b3\u30b6\u30c3\u30af\u914d\u5217\uff09\u304c\u3001\u4e0b\u6d41\u306b\u306f\u8ee2\u5199\u306b\u95a2\u308f\u308b\u30dd\u30eaA\u30b7\u30b0\u30ca\u30eb\uff08PolyA signal\uff09\u304c\u633f\u5165\u3055\u308c\u3066\u3044\u307e\u3059\u3002<br>\u305f\u3060\u3057\u3001Kozak\u914d\u5217\u306f\u30d9\u30af\u30bf\u30fc\u306b\u306f\u633f\u5165\u3055\u308c\u3066\u3044\u306a\u3044\u3053\u3068\u304c\u591a\u304f\u3001Kozak\u914d\u5217\u3092\u4ed8\u3051\u305f\u907a\u4f1d\u5b50\u3092\u30d9\u30af\u30bf\u30fc\u306b\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u3059\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002<br>\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306e\u4f8b\uff1aCMV\u3001EF1\u03b1\u3001SV40\u3001PGK\u3001UbC\u3001CAG\u306a\u3069<\/p>\n\n\n\n<figure class=\"wp-block-image aligncenter\"><img loading=\"lazy\" decoding=\"async\" width=\"493\" height=\"83\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/150959d4ab52349cdf0bcb83e72c980e.png\" alt=\"\u771f\u6838\u751f\u7269\uff08\u54fa\u4e73\u985e\u7d30\u80de\u306a\u3069\uff09\u3067\u306e\u30bf\u30f3\u30d1\u30af\u8cea\u767a\u73fe\u306b\u5fc5\u8981\u306a\u30a8\u30ec\u30e1\u30f3\u30c8\u4f8b\" class=\"wp-image-14086\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/150959d4ab52349cdf0bcb83e72c980e.png 493w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/150959d4ab52349cdf0bcb83e72c980e-300x51.png 300w\" sizes=\"auto, (max-width: 493px) 100vw, 493px\" \/><\/figure>\n\n\n\n<p><\/p>\n\n\n\n<p>SD\u914d\u5217\u304c\u3042\u308c\u3070\u539f\u6838\u751f\u7269\u7528\u767a\u73fe\u30d9\u30af\u30bf\u30fc\u3060\u3068\u4e88\u6e2c\u3055\u308c\u307e\u3059\u304c\u3001\u305d\u308c\u3088\u308a\u3082\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u540d\u3067\u5224\u65ad\u3059\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u306b\u3064\u3044\u3066\u306f\u30d6\u30ed\u30b0\u7b2c2\u56de\u306b\u307e\u3068\u3081\u308b\u306e\u3067\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<h2 class=\"wp-block-heading\" id=\"h-3-\u305d\u306e\u4ed6\u306e\u3088\u304f\u898b\u304b\u3051\u308b\u30a8\u30ec\u30e1\u30f3\u30c8\">3. \u305d\u306e\u4ed6\u306e\u3088\u304f\u898b\u304b\u3051\u308b\u30a8\u30ec\u30e1\u30f3\u30c8<\/h2>\n\n\n\n<figure class=\"wp-block-image aligncenter size-full wp-image-14089\"><img decoding=\"async\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/f470ecac932000fe5a8dfd123084d891.png\" alt=\"pcDNA3.1\u306e\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\uff1a\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u30b5\u30a4\u30c8\" class=\"wp-image-14081\" \/><figcaption class=\"wp-element-caption\"><strong>\u56f34. pcDNA3.1\u306e\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\uff1a\u30b7\u30fc\u30b1\u30f3\u30b9\u7528\u30d7\u30e9\u30a4\u30de\u30fc\u306e\u30a2\u30cb\u30fc\u30ea\u30f3\u30b0\u30b5\u30a4\u30c8<\/strong><\/figcaption><\/figure>\n\n\n\n<p>\u56f34\u306eT7\u306fT7 promoter\u914d\u5217\u3067\u3059\u304c\u3001\u5927\u8178\u83cc\u306epET\u767a\u73fe\u7cfb\u3067\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u3068\u3057\u3066\u4f7f\u7528\u3059\u308b\u305f\u3081\u306e\u3082\u306e\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u3057\u305fDNA\u306e\u914d\u5217\u89e3\u6790\u306bT7 promoter primer\uff08\u3088\u304f\u4f7f\u308f\u308c\u307e\u3059\uff09\u304c\u4f7f\u3048\u308b\u3068\u3054\u7406\u89e3\u304f\u3060\u3055\u3044\u3002\u305d\u306e\u4ed6M13\u30d5\u30a9\u30ef\u30fc\u30c9\u914d\u5217\u3001M13\u30ea\u30d0\u30fc\u30b9\u914d\u5217\u306a\u3069\u30b7\u30fc\u30b1\u30f3\u30b9\u306b\u4fbf\u5229\u306a\u914d\u5217\u304c\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u5468\u8fba\u306b\u633f\u5165\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u591a\u3044\u3067\u3059\u3002\u5927\u62b5\u306e\u5834\u5408\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u914d\u5217\u306e\u30b7\u30fc\u30b1\u30f3\u30b9\uff08\u914d\u5217\u89e3\u6790\uff09\u306f\u5fc5\u9808\u3067\u3059\u306e\u3067\u3001\u3053\u306e\u4fbf\u5229\u306a\u914d\u5217\u306e\u60c5\u5831\u3092\u30de\u30c3\u30d7\u304b\u3089\u8aad\u307f\u53d6\u3063\u3066\u304f\u3060\u3055\u3044\u3002<\/p>\n\n\n\n<p>\u305d\u306e\u4ed6\u7279\u5b9a\u306e\u767a\u73fe\u7cfb\u3060\u3051\u3067\u4f7f\u7528\u3059\u308b\u914d\u5217\u3001\u30a6\u30a4\u30eb\u30b9\u4f5c\u6210\u7528\u306e\u914d\u5217\uff08LTR\u914d\u5217\uff09\u306a\u3069\u304c\u8a18\u8f09\u3055\u308c\u3066\u3044\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u304c\u3001\u5168\u3066\u3092\u899a\u3048\u308b\u3053\u3068\u306f\u52b9\u7387\u7684\u3067\u306f\u306a\u3044\u3068\u601d\u3044\u307e\u3059\u3002\u4e88\u671f\u305b\u306c\u4e0d\u660e\u306a\u30a8\u30ec\u30e1\u30f3\u30c8\u304c\u3042\u308b\u5834\u5408\u306f\u3001\u30d6\u30ed\u30b0\u7b2c2\u56de\u306e\u30a8\u30ec\u30e1\u30f3\u30c8\u30ea\u30b9\u30c8\u3067\u78ba\u8a8d\u3057\u3066\u3082\u3089\u3048\u3070\u3054\u5b89\u5fc3\u3044\u305f\u3060\u3051\u308b\u306f\u305a\u3067\u3059\u3002<\/p>\n\n\n\n<h2 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id=\"h-\u30de\u30c3\u30d7\u89e3\u8aad\u306e\u9053\u7b4b\"><b>\u30de\u30c3\u30d7\u89e3\u8aad\u306e\u9053\u7b4b<\/b><\/h3>\n\n\n\n<p>\u307e\u305a\u306f3\u3064\u306e\u4e3b\u8981\u30a8\u30ec\u30e1\u30f3\u30c8\u3092\u63a2\u3057\u3066\u307f\u3066\u304f\u3060\u3055\u3044\u3002<br>\u5927\u8178\u83cc\u7528\u306e\u6297\u751f\u7269\u8cea\u8010\u6027\u907a\u4f1d\u5b50\u304c\u7279\u5b9a\u3067\u304d\u308c\u3070\u5927\u8178\u83cc\u306b\u5165\u308c\u3066\u5897\u3084\u3059\u3053\u3068\u304c\u3067\u304d\u308b\u306f\u305a\u3067\u3059\u3002<br>\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u304c\u5206\u304b\u308c\u3070\u3001\u3069\u306e\u3088\u3046\u306a\u30e9\u30a4\u30b2\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\u304c\u9078\u629e\u3067\u304d\u308b\u304b\u5206\u304b\u308a\u307e\u3059\u3002<br>\u6b21\u306b\u3001\u30af\u30ed\u30fc\u30cb\u30f3\u30b0\u30b5\u30a4\u30c8\u5468\u8fba\u306e\u30a8\u30ec\u30e1\u30f3\u30c8\u3092\u898b\u308c\u3070\u76ee\u7684\u306e\u767a\u73fe\u7cfb\u3067\u4f7f\u7528\u3067\u304d\u308b\u304b\u78ba\u8a8d\u3067\u304d\u307e\u3059\u3002<\/p>\n\n\n\n<p>\u6700\u5f8c\u306b\u3001\u5f53\u793e\u3067\u306f\u5b9f\u969b\u304a\u6301\u3061\u306e\u30d7\u30e9\u30b9\u30df\u30c9\u30d9\u30af\u30bf\u30fc\u306e\u89e3\u8aad\u3092\u30b5\u30dd\u30fc\u30c8\u81f4\u3057\u3066\u304a\u308a\u307e\u3059\uff01<br>\u30de\u30c3\u30d7\u3092\u30c6\u30af\u30cb\u30ab\u30eb\u30b5\u30dd\u30fc\u30c8\u5b9b\u306b\u304a\u9001\u308a\u304f\u3060\u3055\u3044\u3002<a href=\"mailto:jptech@thermofisher.com?subject=\u304a\u554f\u3044\u5408\u308f\u305b\">jptech@thermofisher.com<\/a><\/p>\n\n\n\n<p>\u7b2c2\u56de\u3067\u306f\u3001\u30d9\u30af\u30bf\u30fc\u30de\u30c3\u30d7\u306b\u542b\u307e\u308c\u308b\u5404\u30a8\u30ec\u30e1\u30f3\u30c8\u306e\u6a5f\u80fd\u3001\u7531\u6765\u751f\u7269\u306a\u3069\u3092\u307e\u3068\u3081\u305f\u8868\u3092\u3054\u6848\u5185\u81f4\u3057\u307e\u3059\u3002\u3088\u308d\u3057\u3051\u308c\u3070\u305d\u3061\u3089\u3082\u4f75\u305b\u3066\u3054\u89a7\u304f\u3060\u3055\u3044\u3002<br><a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/protein-expression-cloning-2-bid-ts-mbr-24076\/?CID=bid_mol_clo_r04_jp_cp0000_pjt0000_bid00000_0so_blg_op_awa_oc_s00_cloning_bid_ts_1_Social_LAB\" target=\"_blank\" 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