{"id":14170,"date":"2023-05-16T17:44:37","date_gmt":"2023-05-16T08:44:37","guid":{"rendered":"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/?p=14170"},"modified":"2025-02-10T14:17:55","modified_gmt":"2025-02-10T05:17:55","slug":"gene-editing_bid_ts_4","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/gene-editing_bid_ts_4\/","title":{"rendered":"\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\uff0fCas9\uff09 \uff5e\u7b2c4\u56de\u3000\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\uff5e"},"content":{"rendered":"

\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u521d\u5fc3\u8005A\u3055\u3093\u306b\u3088\u308b\u300c\u521d\u3081\u3066\u306e\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u300d\u3002
\n\u7b2c1\u56de<\/a>\u3001\u7b2c2\u56de<\/a>\u3001\u7b2c3\u56de<\/a>\u3092\u901a\u3057\u3066\u3001Cas9\u3084\u30ac\u30a4\u30c9RNA\u306e\u4f9b\u4e0e\u65b9\u6cd5\u3068\u7279\u5fb4\u3092\u5b66\u3093\u3060A\u3055\u3093\u306f\u3001Protein\u6cd5\u3092\u5229\u7528\u3057\u3066\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3092\u59cb\u3081\u3088\u3046\u3068\u3057\u3066\u3044\u307e\u3059\u3002\"\"<\/p>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u306f\u3069\u3046\u3044\u3046\u8a66\u85ac\u304c\u3042\u308b\u306e\u304b\uff1f\u30dd\u30a4\u30f3\u30c8\u306f\u3069\u3053\u3067\u3001\u3069\u306e\u3088\u3046\u306b\u9078\u3079\u3070\u3088\u3044\u306e\u304b\u3002A\u3055\u3093\u3068\u5148\u8f29\u306e\u4f1a\u8a71\u3092\u805e\u304d\u306a\u304c\u3089\u5b66\u3093\u3067\u3044\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u65b9\u6cd5\u306f\u4e3b\u306b3\u7a2e\u985e<\/h2>\n

\"\"A\u3055\u3093<\/span>\uff1a
\nCas9\u3068\u30ac\u30a4\u30c9RNA\u3092\u7528\u610f\u3057\u307e\u3057\u305f\uff01
\n\u3053\u306e2\u3064\u3092\u7d30\u80de\u306b\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u305f\u3044\u3067\u3059<\/p>\n

\u5148\u8f29<\/span>\uff1a
\n\u305d\u3046\u3060\u306d\u3002\u3067\u306f\u554f\u984c\u3067\u3059\u3002\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u306f\u3069\u3046\u3044\u3046\u65b9\u6cd5\u304c\u3042\u308b\u3067\u3057\u3087\u3046\u304b\uff1f<\/p>\n

A\u3055\u3093<\/span>\uff1a
\n\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3068\u2026\u3001\u3042\u3068\u4f55\u3067\u3057\u305f\u3063\u3051\uff1f<\/p>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u306eCas9\u3068\u30ac\u30a4\u30c9RNA\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u306f\u3001A\u3055\u3093\u3082\u77e5\u3063\u3066\u3044\u305f\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3092\u542b\u3081\u3001\u4e3b\u306b3\u7a2e\u985e\u306e\u65b9\u6cd5\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\uff1c\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\uff1e<\/b>
\n\u30ab\u30c1\u30aa\u30f3\u6027\u8102\u8cea\u5c0e\u5165\u6cd5\u3068\u3082\u547c\u3070\u308c\u307e\u3059\u3002\u30ab\u30c1\u30aa\u30f3\uff08\u30d7\u30e9\u30b9\u96fb\u8377\uff09\u3092\u6301\u3064\u8102\u8cea\u3068\u6838\u9178\u306e\u8907\u5408\u4f53\u3092\u5f62\u6210\u3055\u305b\u3001\u7d30\u80de\u306b\u6dfb\u52a0\u3059\u308b\u65b9\u6cd5\u3067\u3059\u3002\u7279\u5225\u306a\u6a5f\u5668\u3092\u4f7f\u7528\u3057\u306a\u3044\u305f\u3081\u3001\u6bd4\u8f03\u7684\u5b89\u4fa1\u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u304c\u3067\u304d\u307e\u3059\u3002<\/p>\n

\uff1c\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\uff1e<\/b>
\n\u96fb\u6c17\u7a7f\u5b54\u6cd5\u3068\u3082\u547c\u3070\u308c\u307e\u3059\u3002\u96fb\u6c17\u30d1\u30eb\u30b9\u306b\u3088\u308a\u7d30\u80de\u819c\u306b\u5f62\u6210\u3057\u305f\u5b54\u3092\u4ecb\u3057\u3066\u3001\u6838\u9178\u306a\u3069\u3092\u7d30\u80de\u5185\u306b\u901a\u904e\u3055\u305b\u308b\u7269\u7406\u7684\u306a\u65b9\u6cd5\u3067\u3059\u3002\u6a5f\u5668\u3092\u4f7f\u7528\u3057\u307e\u3059\u304c\u3001\u5c0e\u5165\u52b9\u7387\u304c\u9ad8\u304f\u306a\u308a\u3084\u3059\u3044\u624b\u6cd5\u3067\u3059\u3002<\/p>\n

\uff1c\u30a6\u30a4\u30eb\u30b9\u6cd5\uff1e<\/b>
\n\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u306f\u5c0e\u5165\u56f0\u96e3\u306a\u7d30\u80de\u3084in vivo\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306b\u3088\u304f\u7528\u3044\u3089\u308c\u307e\u3059\u3002<\/p>\n

3\u3064\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306e\u3046\u3061\u3001\u9078\u3070\u308c\u308b\u3053\u3068\u304c\u591a\u3044\u306e\u306f\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5<\/b>\u3067\u3059\u3002\u7406\u7531\u306f\u3001\u7c21\u4fbf\u3067\u3042\u308b\u4e0a\u306b\u3001\u5b9f\u7e3e\u306e\u3042\u308b\u7d30\u80de\u3082\u591a\u3044\u304b\u3089\u3067\u3059\u3002\u305f\u3060\u3001\u3082\u3061\u308d\u3093\u4e07\u80fd\u3067\u306f\u3042\u308a\u307e\u305b\u3093\u3002\u6d6e\u904a\u7d30\u80de\u3084\u795e\u7d4c\u7d30\u80de\u306a\u3069\u306b\u306f\u3001\u5c0e\u5165\u304c\u96e3\u3057\u3044\u7d30\u80de\u304c\u591a\u3044\u3053\u3068\u3082\u77e5\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3053\u306e\u3088\u3046\u306a\u7d30\u80de\u306b\u306f\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u65b9\u6cd5\u3084\u30a6\u30a4\u30eb\u30b9\u6cd5\u3067\u5c0e\u5165\u3059\u308b\u3068\u52b9\u7387\u304c\u9ad8\u304f\u306a\u308a\u307e\u3059\u3002<\/p>\n

DNA\u6cd5\u3001RNA\u6cd5\u3001Protein\u6cd5\u3054\u3068\u306b\u6700\u9069\u306a\u8a66\u85ac\u3092\u4f7f\u304a\u3046<\/h2>\n

A\u3055\u3093<\/span>\uff1a
\n\u7c21\u5358\u305d\u3046\u3060\u3057\u3001\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306b\u3057\u307e\u3059\uff01
\n\u8a66\u85ac\u304c\u3044\u304f\u3064\u304b\u3042\u308b\u3051\u3069\u3001\u3069\u308c\u304c\u3044\u3044\u3093\u3060\u308d<\/p>\n

\u5148\u8f29<\/span>\uff1a
\nDNA\u65b9\u6cd5\u3001RNA\u65b9\u6cd5\u3001Protein\u6cd5\u305d\u308c\u305e\u308c\u306b\u9069\u3057\u305f\u8a66\u85ac\u304c\u3042\u308b\u3088<\/p>\n

\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306b\u306f\u3001\u7b2c3\u56de<\/a>\u3067\u3054\u7d39\u4ecb\u3057\u305f\u30b2\u30ce\u30e0\u7de8\u96c6\u306e3\u3064\u306e\u65b9\u6cd5\u3001\u3064\u307e\u308aDNA\u65b9\u6cd5\u3001RNA\u65b9\u6cd5\u3001Protein\u65b9\u6cd5\u306e3\u7a2e\u985e\u305d\u308c\u305e\u308c\u306e\u65b9\u6cd5\u306b\u9069\u3057\u305f\u8a66\u85ac<\/b>\u304c\u3042\u308a\u307e\u3059\u3002\u88681\u3092\u53c2\u8003\u306b\u3057\u3066\u3001\u8a66\u85ac\u3092\u9078\u629e\u3057\u307e\u3057\u3087\u3046\u3002<\/p>\n\n\n\n\n\n\n
\u88681\u3000\u30b2\u30ce\u30e0\u7de8\u96c6\u306e3\u3064\u306e\u4f9b\u4e0e\u65b9\u6cd5<\/caption>\n
\u4f9b\u4e0e\u65b9\u6cd5<\/td>\nDNA\u65b9\u6cd5<\/td>\nRNA\u65b9\u6cd5<\/td>\nProtein\u65b9\u6cd5<\/td>\n<\/tr>\n<\/thead>\n
\u7d30\u80de\u5185\u306b\u5165\u308c\u308b\u3082\u306e<\/td>\nCRISPR vector\"CRISPR<\/td>\n\u30ac\u30a4\u30c9RNA\u3001Cas9 mRNA\"\u30ac\u30a4\u30c9RNA\u3001Cas9<\/td>\n\u30ac\u30a4\u30c9RNA\u3001Cas9\u30bf\u30f3\u30d1\u30af\u8cea\"\u30ac\u30a4\u30c9RNA\u3001Cas9\u30bf\u30f3\u30d1\u30af\u8cea\"<\/td>\n<\/tr>\n
\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306e\u8a66\u85ac<\/td>\n\"\"Invitrogen\u2122 Lipofectamine\u2122 3000 Transfection Reagent<\/a><\/td>\n\"\"Invitrogen\u2122 Lipofectamine\u2122 MessengerMAX\u2122 Transfection Reagent<\/a><\/td>\n\"\"Invitrogen\u2122 Lipofectamine\u2122 CRISPRMAX\u2122 Cas9 Transfection Reagent<\/a><\/td>\n<\/tr>\n<\/tbody>\n<\/table>\n

A\u3055\u3093<\/span>\uff1a
\n\u3042\u3063\uff01\u3067\u3082\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u50d5\u306e\u7d30\u80de\u306b\u3061\u3083\u3093\u3068\u5165\u308b\u306e\u304b\u306a\uff1f<\/p>\n

\u5148\u8f29<\/span>\uff1a
\n\u3044\u3044\u3068\u3053\u308d\u306b\u6c17\u304c\u3064\u3044\u305f\u306d\uff01
\n\u305d\u3046\u3001\u5c0e\u5165\u52b9\u7387\u306f\u7d30\u80de\u306e\u7a2e\u985e\u306b\u3088\u3063\u3066\u5927\u304d\u304f\u7570\u306a\u308b<\/b><\/span>\u3093\u3060<\/p>\n

\u305d\u3046\u3001\u7c21\u5358\u3060\u304b\u3089\u3068\u3044\u3046\u7406\u7531\u3067A\u3055\u3093\u306f\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3092\u9078\u3093\u3060\u3088\u3046\u3067\u3059\u304c\u3001\u5927\u5207\u306a\u306e\u306f\u300c\u305d\u3082\u305d\u3082\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u305d\u306e\u7d30\u80de\u3078\u306e\u5c0e\u5165\u52b9\u7387\u304c\u9ad8\u3044\u304b\u3069\u3046\u304b\u300d\u3067\u3059\u3002\u5c0e\u5165\u52b9\u7387\u306f\u300c\u7d30\u80de\u306e\u7a2e\u985e\u300d<\/b>\u306b\u3088\u3063\u3066\u5927\u304d\u304f\u5909\u308f\u308b\u304b\u3089\u3067\u3059\u3002\u88682\u3001\u88683\u3001\u88684\u306b\u3001\u305d\u308c\u305e\u308cLipofectamine 3000\u3001LipofectamineT<\/sup> MessengerMAX\u3001Lipofectamine CRISPRMAX Transfection Reagent\u306e\u7d30\u80de\u3054\u3068\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u3092\u793a\u3057\u307e\u3057\u305f\u3002\u4f7f\u7528\u3057\u305f\u3044\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u8a66\u85ac\u3068\u7d30\u80de\u306e\u7d44\u307f\u5408\u308f\u305b\u3067\u3001\u5c0e\u5165\u52b9\u7387\u304c\u4f55%\u304f\u3089\u3044\u306a\u306e\u304b\u3001\u78ba\u8a8d\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u88682\uff1aLipofectamine 3000 Transfection Reagent\u3092\u7528\u3044\u305f\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387
\n\u4e0b\u8868\u306f\u4e00\u90e8\u629c\u7c8b<\/u><\/b>\u3067\u3059\u3002\u307b\u304b\u306b\u3082\u591a\u304f\u306e\u7d30\u80de\u682a\u306e\u52b9\u7387\u3092\u8abf\u3079\u3066\u3044\u307e\u3059\u306e\u3067\u3001\u3053\u3061\u3089<\/a>\u3092\u3054\u89a7\u304f\u3060\u3055\u3044\u3002\"Lipofectamine<\/p>\n

\u88683\uff1aLipofectamine MessengerMAX Transfection Reagent\u3092\u7528\u3044\u305fmRNA\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\"Lipofectamine<\/p>\n

\u88684\uff1aLipofectamine CRISPRMAX Transfection Reagent\u3092\u7528\u3044\u3066Cas9 \u30bf\u30f3\u30d1\u30af\u8cea\u3068HPRT sgRNA\u3092\u5c0e\u5165\u3057\u305f\u5834\u5408\u306e\u5909\u7570\u5c0e\u5165\u52b9\u7387
\n\"Lipofectamine<\/p>\n

\u88682\uff5e4\u306b\u60c5\u5831\u304c\u306a\u304b\u3063\u305f\u5834\u5408\u306f\u3001\u7528\u3044\u305f\u3044\u7d30\u80de\u3067\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u5b9f\u7e3e\u304c\u3042\u308b\u304b\u3001\u6587\u732e\u3067\u3082\u8abf\u3079\u3066\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u5148\u8f29<\/span>\uff1a
\n\u6587\u732e\u3067\u306f\u3001\u5fc5\u305a\u300c\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u5c0e\u5165\u5b9f\u7e3e\u300d<\/b><\/span>\u3092\u78ba\u8a8d\u3057\u3066\u306d\u3002
\n\u305f\u3068\u3048\u3070siRNA\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u65b9\u6cd5\u304c\u30b2\u30ce\u30e0\u7de8\u96c6\u3067\u3082\u6700\u9069\u3068\u306f\u9650\u3089\u306a\u3044\u3088<\/p>\n

\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u52b9\u7387\u304c\u4f4e\u3044\u3068\u304d\u306e\u624b\u6bb5\u306f\uff1f<\/h2>\n

A\u3055\u3093<\/span>\uff1a
\n\u88682\u3084\u88684\u3092\u898b\u3066\u3044\u308b\u3068\u3001\u305f\u3068\u3048\u3070Jurkat\u306f\u5c0e\u5165\u52b9\u7387\u304c\u4f4e\u3044\u3093\u3067\u3059\u306d\u3002
\n\u3053\u3046\u3044\u3046\u3068\u304d\u3001\u3069\u3046\u3059\u308c\u3070\u3044\u3044\u3093\u3060\u308d\u3046\uff1f<\/p>\n

\u5148\u8f29<\/span>\uff1a
\n\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u5c0e\u5165\u52b9\u7387\u304c\u4f4e\u3044\u3068\u304d\u306f\u3001
\n\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5<\/b><\/span>\u304b\u3001\u30a6\u30a4\u30eb\u30b9\u6cd5<\/b><\/span>\u304c\u304a\u3059\u3059\u3081\u3060\u3088<\/p>\n

\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u3082\u5c0e\u5165\u52b9\u7387\u304c\u4f4e\u3044\u5834\u5408\u306f\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u304b\u30a6\u30a4\u30eb\u30b9\u6cd5\u3067\u5c0e\u5165\u52b9\u7387\u304c\u6539\u5584\u3055\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002<\/p>\n

\u307e\u305a\u306f2\u3064\u306e\u65b9\u6cd5\u306e\u5dee\u3001\u3064\u307e\u308a\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u3068\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306e\u5c0e\u5165\u52b9\u7387\u3092\u305d\u308c\u305e\u308c\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u88685\u306b\u3001Protein\u6cd5\u3067\u304a\u3059\u3059\u3081\u306eLipofectamine CRISPRMAX Transfection Reagent\u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u305f\u7d50\u679c\u3068\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3057\u305f\u3068\u304d\u306e\u5909\u7570\u5c0e\u5165\u52b9\u7387\u3092\u307e\u3068\u3081\u307e\u3057\u305f\u3002<\/p>\n

\u88685\uff1aLipofectamine CRISPRMAX Transfection Reagent\u3068\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306e\u5909\u7570\u5c0e\u5165\u52b9\u7387
\n\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306e\u5c0e\u5165\u52b9\u7387\u306f\u3001\u5f53\u793e\u306eInvitrogen\u2122 Neon\u2122 Transfection System<\/a>\u3092\u7528\u3044\u3066\u78ba\u8a8d\u3057\u307e\u3057\u305f\u3002\"Lipofectamine<\/p>\n

\u305f\u3068\u3048\u3070\u88685\u306e293FT\u3001mESC\u306e\u5c0e\u5165\u52b9\u7387\u3092\u898b\u3066\u307f\u307e\u3057\u3087\u3046\u3002\u3053\u308c\u3089\u306fLipofectamine CRISPRMAX Transfection Reagent \u3067\u3082\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u3067\u3082\u5c0e\u5165\u52b9\u7387\u306b\u5927\u304d\u306a\u5dee\u306f\u306a\u304f\u3001\u3069\u3061\u3089\u306e\u65b9\u6cd5\u3067\u308270\uff5e80%\u4ee5\u4e0a\u306e\u5c0e\u5165\u52b9\u7387\u304c\u5f97\u3089\u308c\u3066\u3044\u307e\u3059\u3002\u3057\u304b\u3057\u3001\u4f8b\u3048\u3070LipofectamineCRISPRMAX Transfection Reagent \u3067\u306f19%\u3057\u304b\u5c0e\u5165\u3055\u308c\u306a\u3044Jurkat\u3067\u3082\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u3092\u7528\u3044\u308b\u306894%\u306e\u5c0e\u5165\u52b9\u7387\u304c\u5f97\u3089\u308c\u3066\u3044\u307e\u3059\u3002
\n\u3053\u306e\u3088\u3046\u306b\u3001\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u304c\u96e3\u3057\u3044\u7d30\u80de\u306e\u5834\u5408\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u3067\u306f\u9ad8\u3044\u5c0e\u5165\u52b9\u7387\u304c\u5f97\u3089\u308c\u308b\u3053\u3068\u304c\u3042\u308a\u307e\u3059\u3002\u306a\u304a\u3001\u304b\u3064\u3066\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u306f\u7d30\u80de\u3054\u3068\u306b\u6761\u4ef6\u691c\u8a0e\u304c\u5fc5\u8981\u3067\u5927\u5909\u3068\u8a00\u308f\u308c\u3066\u3044\u307e\u3057\u305f\u304c\u3001\u73fe\u5728\u306fNeon Transfection System\u306e\u3088\u3046\u306b100\u4ee5\u4e0a\u306e\u7d30\u80de\u682a\u306b\u5bfe\u3057\u3066\u6761\u4ef6\u304c\u6700\u9069\u5316<\/a>\u6e08\u307f\u306e\u88c5\u7f6e\u3082\u3042\u308a\u307e\u3059\u3002\u3053\u3046\u3044\u3063\u305f\u88c5\u7f6e\u3092\u7528\u3044\u308c\u3070\u8ff7\u308f\u305a\u5b9f\u9a13\u3092\u9032\u3081\u3089\u308c\u308b\u5b89\u5fc3\u611f\u3082\u5f97\u3089\u308c\u307e\u3059\u3002<\/p>\n

\u6b21\u306b\u3001\u30a6\u30a4\u30eb\u30b9\u6cd5\u3067\u306f\u3001\u3042\u3089\u304b\u3058\u3081\u30c7\u30b6\u30a4\u30f3\u6e08\u307f\u306e\u30ac\u30a4\u30c9RNA\u3092\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u3057\u305f\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9<\/a>\u3001\u304a\u3088\u3073Cas9\u304c\u30d1\u30c3\u30b1\u30fc\u30b8\u30f3\u30b0\u3055\u308c\u305f\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9<\/a>\u3082\u8ca9\u58f2\u3057\u3066\u3044\u307e\u3059\u3002\u30c7\u30b6\u30a4\u30f3\u6e08\u307f\u306e\u30ac\u30a4\u30c9RNA\u306f\u3001Gene Symbol\u306a\u3069\u3092\u5165\u529b\u3057\u3066\u3001\u30bf\u30fc\u30b2\u30c3\u30c8\u306b\u5408\u3063\u305f\u3082\u306e\u3092\u30c7\u30b6\u30a4\u30f3\u3057\u307e\u3057\u3087\u3046\u3002
\n\u305f\u3060\u3057\u3001\u30a6\u30a4\u30eb\u30b9\u6cd5\u306e\u5c0e\u5165\u52b9\u7387\u3082\u7d30\u80de\u306b\u3088\u3063\u3066\u5927\u304d\u304f\u7570\u306a\u308a\u307e\u3059\u3002\u305d\u306e\u305f\u3081\u3001\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u5b9f\u9a13\u524d\u306b\u3001GFP\u4ed8\u304d\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/a>\u3068\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb<\/a>\u306egRNA\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u3067\u5c0e\u5165\u52b9\u7387\u3092\u3042\u3089\u304b\u3058\u3081\u78ba\u8a8d\u3057\u3066\u304a\u304d\u307e\u3057\u3087\u3046\u3002<\/p>\n

\"GFP\u6a19\u8b58\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08\u5de6\uff09\u3068\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08\u53f3\uff09\u306e\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u3092\u5c0e\u5165\u3057\u305fHT1080\u7d30\u80de\"

\u56f31\uff1aGFP\u6a19\u8b58\u306e\u30dd\u30b8\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08\u5de6\uff09\u3068\u30cd\u30ac\u30c6\u30a3\u30d6\u30b3\u30f3\u30c8\u30ed\u30fc\u30eb\uff08\u53f3\uff09\u306e\u30ec\u30f3\u30c1\u30a6\u30a4\u30eb\u30b9\u3092\u5c0e\u5165\u3057\u305fHT1080\u7d30\u80de<\/p><\/div>\n

A\u3055\u3093<\/span>\uff1a
\n\u3088\u3057\u3002\u6e96\u5099\u3082\u6574\u3063\u305f\u3057\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3067\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u3059\u308b\u305e\uff01\u3000\u3046\u307e\u304f\u3044\u304f\u3068\u3044\u3044\u306a\u3041<\/p>\n

\u307e\u3068\u3081<\/h2>\n

\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u306f\u3001\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u3001\u30a8\u30ec\u30af\u30c8\u30ed\u30dd\u30ec\u30fc\u30b7\u30e7\u30f3\u6cd5\u3001\u30a6\u30a4\u30eb\u30b9\u6cd5\u306e3\u7a2e\u985e\u304c\u3088\u304f\u5229\u7528\u3055\u308c\u3066\u3044\u307e\u3059\u3002\u30ea\u30dd\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u6cd5\u306fDNA\u6cd5\u3001RNA\u6cd5\u3001Protein\u6cd5\u305d\u308c\u305e\u308c\u306b\u9069\u3057\u305f\u8a66\u85ac\u304c\u3042\u308b\u306e\u3067\u3001\u305d\u308c\u3092\u9078\u3073\u307e\u3057\u3087\u3046\u3002\u6ce8\u610f\u70b9\u306f\u3069\u306e\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\u65b9\u6cd5\u3082\u7d30\u80de\u306b\u3088\u3063\u3066\u5c0e\u5165\u7387\u304c\u7570\u306a\u308b\u70b9\u3067\u3059\u3002\u3057\u3063\u304b\u308a\u5b9f\u7e3e\u3092\u8abf\u3079\u3066\u5b9f\u9a13\u306b\u81e8\u307f\u307e\u3057\u3087\u3046\u3002<\/p>\n

\u6b21\u56de<\/a>\u306f\u3001A\u3055\u3093\u3082\u5fc3\u914d\u3057\u3066\u3044\u308b\u3001\u300c\u3046\u307e\u304f\u3044\u3063\u305f\u304b\u3069\u3046\u304b\u300d\u3001\u3064\u307e\u308a\u3001\u5909\u7570\u306e\u5c0e\u5165\u52b9\u7387\u3092\u8a55\u4fa1\u3059\u308b\u65b9\u6cd5\u306b\u3064\u3044\u3066\u3054\u7d39\u4ecb\u3057\u307e\u3059\u3002<\/p>\n

\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\u30b7\u30ea\u30fc\u30ba\uff1e
\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c1\u56de\u3000CRISPR\/Cas9\u30b7\u30b9\u30c6\u30e0\u306e\u539f\u7406\uff5e<\/a>
\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c2\u56de\u3000\u30ac\u30a4\u30c9RNA\u306e\u30c7\u30b6\u30a4\u30f3\uff5e<\/a>
\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c3\u56de\u30003\u3064\u306eCRIPSR\/Cas9\u5b9f\u9a13\u7cfb\uff5e<\/a>
\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c4\u56de\u3000\u30c8\u30e9\u30f3\u30b9\u30d5\u30a7\u30af\u30b7\u30e7\u30f3\uff5e
\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c5\u56de\u3000\u5909\u7570\u306e\u78ba\u8a8d\u65b9\u6cd5\uff5e<\/a>
\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c6\u56de\u3000\u30aa\u30d5\u30bf\u30fc\u30b2\u30c3\u30c8\u52b9\u679c\uff5e<\/a>
\n\u305d\u3046\u3044\u3046\u3053\u3068\u3060\u3063\u305f\u306e\u304b ! \u30b2\u30ce\u30e0\u7de8\u96c6\u5b9f\u9a13\uff08CRISPR\/Cas9\uff09 \uff5e\u7b2c7\u56de\u3000\u30ce\u30c3\u30af\u30a4\u30f3\uff5e<\/a><\/p>\n

\uff1c\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u57fa\u790e\u7684\u306a\u5185\u5bb9\uff1e
\n\u30b2\u30ce\u30e0\u7de8\u96c6\u306e\u539f\u7406\u3068\u624b\u5f15\u304d<\/a><\/p>\n

CRISPR\/Cas9 \u30b9\u30bf\u30fc\u30bf\u30fc\u30d1\u30c3\u30af\u3067\u5b9f\u969b\u306b\u30b2\u30ce\u30e0\u7de8\u96c6\u3092\u8a66\u3057\u3066\u307f\u3088\u3046\uff01\uff01<\/h3>\n

\u8a73\u3057\u304f\u306f\u3053\u3061\u3089<\/a><\/p>\n

 <\/p>\n

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