{"id":14355,"date":"2023-05-31T11:52:59","date_gmt":"2023-05-31T02:52:59","guid":{"rendered":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/?p=14355"},"modified":"2025-04-15T16:09:21","modified_gmt":"2025-04-15T07:09:21","slug":"rna_workshop_bid_ts_4","status":"publish","type":"post","link":"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/rna_workshop_bid_ts_4\/","title":{"rendered":"\u3010\u3084\u3063\u3066\u307f\u305f\u3011RNA\u3092\u5e38\u6e29\u3067\u653e\u7f6e\u3057\u3066\u307f\u305f\uff08\u7d9a\u7de8\uff1a0\uff5e2\u9031\u9593\uff09"},"content":{"rendered":"<h2>\u306f\u3058\u3081\u306b<\/h2>\n<p>RNA\u306f\u5206\u89e3\u3057\u3084\u3059\u3044\u3082\u306e\u3002\u307f\u306a\u3055\u307e\u305d\u3093\u306a\u30a4\u30e1\u30fc\u30b8\u3092\u304a\u6301\u3061\u306e\u65b9\u304c\u307b\u3068\u3093\u3069\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002\u4ee5\u524d\u306e\u8a18\u4e8b\uff08<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/rna_workshop_gsd_ts_1\/?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_rna_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u3010\u3084\u3063\u3066\u307f\u305f\u3011RNA\u3092\u5e38\u6e29\u3067\u653e\u7f6e\u3057\u3066\u307f\u305f<\/a>\uff09\u3067\u306f\u3001\u3069\u308c\u304f\u3089\u3044\u5206\u89e3\u3057\u3084\u3059\u3044\u304b\u3092\u8abf\u3079\u308b\u305f\u3081\u3001\u5e38\u6e29\u307e\u305f\u306f70\u2103\u3067\u3001\u6700\u95774\u6642\u9593\u307e\u3067RNA\u3092\u653e\u7f6e\u3057\u3066\u307f\u305f\u7d50\u679c\u3092\u7d39\u4ecb\u3057\u307e\u3057\u305f\u3002\u8a73\u7d30\u306f\u4ee5\u524d\u306e\u8a18\u4e8b\u3092\u53c2\u7167\u3044\u305f\u3060\u304f\u3068\u3057\u3066\u3001\u5e38\u6e29\u3067\u306f4\u6642\u9593\u653e\u7f6e\u3057\u3066\u3082\u307e\u3063\u305f\u304f\u3068\u3044\u3063\u3066\u3044\u3044\u307b\u3069\u5206\u89e3\u306f\u898b\u3089\u308c\u305a\u3001\u3054\u89a7\u3044\u305f\u3060\u3044\u305f\u591a\u304f\u306e\u65b9\u306f\u300c\u610f\u5916\u306b\u5927\u4e08\u592b\u300d\u3068\u3044\u3046\u5370\u8c61\u3092\u304a\u6301\u3061\u306b\u306a\u3063\u305f\u306e\u3067\u306f\u306a\u3044\u3067\u3057\u3087\u3046\u304b\u3002<br \/>\n\u305f\u3060\u3001\u305d\u3053\u3067\u6c17\u306b\u306a\u308b\u306e\u306f\u300c\u3082\u3063\u3068\u9577\u304f\u653e\u7f6e\u3057\u305f\u3089\u3069\u3046\u306a\u308b\u304b\uff1f\u300d\u3068\u3044\u3046\u3053\u3068\u3067\u3059\u3002\u305d\u3053\u3067\u7d9a\u7de8\u3068\u3057\u3066\u3001\u4eca\u56de\u306f2\u9031\u9593\u307e\u3067\u5ef6\u9577\u3057\u3066\u653e\u7f6e\u3057\u3066\u307f\u307e\u3057\u305f\uff01<\/p>\n<h2>\u6750\u6599\u3068\u65b9\u6cd5<\/h2>\n<p>\u6750\u6599\uff1a<\/p>\n<ul>\n<li>RNA\u30b5\u30f3\u30d7\u30eb\uff08HeLa\u7d30\u80de\u304b\u3089Invitrogen\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/dna-rna-purification-analysis\/rna-extraction\/rna-types\/total-rna-extraction\/purelink-rna-mini-kit.html?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_rna_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">PureLink\u2122 RNA mini kit<\/a>\u3067\u7cbe\u88fd\u3057\u305ftotal RNA 5 ng\/\u03bcL\uff09<\/li>\n<li>Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/reagents-kits\/taqman-master-mixes.html?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_rna_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">TaqMan\u2122 Fast Virus 1-Step Master Mix<\/a><\/li>\n<li>Applied Biosystems <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-assays\/taqman-gene-expression.html?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_rna_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">TaqMan\u2122 Gene Expression Assays<\/a>\uff0818S rRNA\u3001Beta catenin)<\/li>\n<\/ul>\n<p>\u653e\u7f6e\u6761\u4ef6\uff1a<\/p>\n<ul>\n<li>RNA\u30b5\u30f3\u30d7\u30eb\u3092\u5e38\u6e29\u3067\u653e\u7f6e\uff08\u653e\u7f6e\u4e2d\u306e\u6e29\u5ea6\u5e45\u306f20\uff5e23\u2103\uff09<\/li>\n<li>\u653e\u7f6e\u6642\u9593\uff1a0\u6642\u9593\uff5e2\u9031\u9593\uff080\u6642\u9593\u30014\u6642\u9593\u30018\u6642\u9593\u300124\u6642\u9593\u300148\u6642\u9593\u300172\u6642\u9593\u30017\u65e5\u9593\u300114\u65e5\u9593\uff09<\/li>\n<\/ul>\n<p>\u5206\u89e3\u5ea6\u306e\u8a55\u4fa1\u65b9\u6cd5\uff1a<\/p>\n<ul>\n<li>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\uff08Applied Biosystems\u2122 <a href=\"https:\/\/www.thermofisher.com\/jp\/ja\/home\/life-science\/pcr\/real-time-pcr\/real-time-pcr-instruments\/step-one-real-time-pcr-systems.html?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_rna_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">StepOnePlus Real-Time PCR Systems<\/a>\uff09\u3068\u3001Agilent\u2122 \u793e\u88fdBioAnalyzer\u2122 \u3067\u6e2c\u5b9a<\/li>\n<\/ul>\n<h2>\u7d50\u679c<\/h2>\n<p>\u3055\u3063\u305d\u304f\u3067\u3059\u304c\u3001\u7d50\u679c\u3067\u3059\u3002<br \/>\n\u5e38\u6e29\u3067\u653e\u7f6e\u3057\u305f\u3068\u3053\u308d\u3001\u521d\u65e5\u306e\u3046\u3061\u306f\u5206\u89e3\u306f\u307b\u3068\u3093\u3069\u898b\u3089\u308c\u307e\u305b\u3093\u3067\u3057\u305f\u304c\u3001\u305d\u308c\u4ee5\u964d\u3067\u306f\u5f90\u3005\u306b\u5206\u89e3\u3057\u300114\u65e5\u5f8c\u3067\u306fRIN\u5024\u304c2.9\u307e\u3067\u4e0b\u304c\u308a\u3001\u660e\u3089\u304b\u306a\u5206\u89e3\u304c\u8a8d\u3081\u3089\u308c\u307e\u3057\u305f\uff08\u56f31\u3001\u56f32\uff09\u3002<\/p>\n<div id=\"attachment_14359\" style=\"width: 773px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14359\" class=\"size-full wp-image-14359\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/795316b92fc766b0181f6fef074f03fa.png\" alt=\"\u6642\u9593\u7d4c\u904e\u3068RIN\u5024\" width=\"763\" height=\"405\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/795316b92fc766b0181f6fef074f03fa.png 763w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/795316b92fc766b0181f6fef074f03fa-300x159.png 300w\" sizes=\"auto, (max-width: 763px) 100vw, 763px\" \/><p id=\"caption-attachment-14359\" class=\"wp-caption-text\">\u56f31.\u6642\u9593\u7d4c\u904e\u3068RIN\u5024<\/p><\/div>\n<p>BioAnalyzer\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\u3092\u898b\u3066\u3082\u3001\u653e\u7f6e0\u6642\u9593\u306b\u5bfe\u3057\u3001\u653e\u7f6e14\u65e5\u9593\u3067\u306f\u5c0f\u3055\u3044\u65ad\u7247\u304c\u591a\u304f\u898b\u3089\u308c\u3001\u660e\u3089\u304b\u306b\u5206\u89e3\u3057\u3066\u3044\u308b\u3053\u3068\u304c\u308f\u304b\u308a\u307e\u3059\uff08\u56f32\uff09\u3002<\/p>\n<div id=\"attachment_14360\" style=\"width: 1089px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14360\" class=\"size-full wp-image-14360\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/2b530e80c7d0de90885e285c5d798063.png\" alt=\"RNA\u30b5\u30f3\u30d7\u30eb\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0\" width=\"1079\" height=\"218\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/2b530e80c7d0de90885e285c5d798063.png 1079w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/2b530e80c7d0de90885e285c5d798063-300x61.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/2b530e80c7d0de90885e285c5d798063-1024x207.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/2b530e80c7d0de90885e285c5d798063-768x155.png 768w\" sizes=\"auto, (max-width: 1079px) 100vw, 1079px\" \/><p id=\"caption-attachment-14360\" class=\"wp-caption-text\">\u56f32\uff0eRNA\u30b5\u30f3\u30d7\u30eb\u306e\u30a8\u30ec\u30af\u30c8\u30ed\u30d5\u30a7\u30ed\u30b0\u30e9\u30e0<\/p><\/div>\n<p>\u524d\u56de\u306e\u7d50\u679c\u3068\u6bd4\u3079\u308b\u3068\u3001\u3061\u3087\u3046\u306970\u2103\u30672\u6642\u9593\u51e6\u7406\u3057\u305fRNA\u304c\u3001\u5e38\u6e29\u306714\u65e5\u9593\u653e\u7f6e\u3057\u305fRNA\u3068\u540c\u7b49\u306e\u5206\u89e3\u3092\u793a\u3057\u307e\u3057\u305f\u3002<\/p>\n<p>\u7d9a\u3044\u3066\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7d50\u679c\u3067\u3059\u3002\u653e\u7f6e0\u6642\u9593\u306eRNA\uff08\u8d64\u7dda\uff09\u306814\u65e5\u9593\u306eRNA\uff08\u9752\u7dda\uff09\u306f\u308f\u305a\u304b\u306b\u305a\u308c\u3066\u3044\u308b\u3082\u306e\u306e\u3001\u305d\u306e\u5dee\u3067\u3042\u308b\u0394C<sub>T<\/sub>\u5024\u306f\u5c0f\u3055\u304f\u3001\u5206\u89e3\u306e\u5f71\u97ff\u306f\u9650\u5b9a\u7684\u3067\u3057\u305f\uff08\u56f33\uff09\u300218S rRNA\u306e\u65b9\u304c\u0394C<sub>T<\/sub>\u5024\u304c\u5927\u304d\u3044\u306e\u306f\u3001\u5897\u5e45\u7523\u7269\u9577\u304cBeta catenin\u3088\u308a\u3082\u9577\u3044\u305f\u3081\u3067\u3059\uff08\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u30c7\u30fc\u30bf\u306e\u89e3\u91c8\u306f<a href=\"https:\/\/www.thermofisher.com\/blog\/learning-at-the-bench\/rna_workshop_gsd_ts_1\/?CID=bid_cbu_sbu_r04_jp_cp0000_pjt0000_bid00000_0so_blg_rna_workshop_bid_ts_4_Social_LAB\" target=\"_blank\" rel=\"noopener\">\u524d\u56de\u306e\u8a18\u4e8b<\/a>\u3092\u3054\u53c2\u7167\u304f\u3060\u3055\u3044\uff09\u3002<\/p>\n<div id=\"attachment_14361\" style=\"width: 1294px\" class=\"wp-caption aligncenter\"><img loading=\"lazy\" decoding=\"async\" aria-describedby=\"caption-attachment-14361\" class=\"size-full wp-image-14361\" src=\"http:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/c8856789ec11ab8b1013037cef6929f9.png\" alt=\"\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5897\u5e45\u66f2\u7dda\u30020\u6642\u9593\uff08\u8d64\u7dda\uff09\u306814\u65e5\u9593\uff08\u9752\u7dda\uff09\u653e\u7f6e\u3057\u305fRNA\u306e\u7d50\u679c\" width=\"1284\" height=\"377\" srcset=\"https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/c8856789ec11ab8b1013037cef6929f9.png 1284w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/c8856789ec11ab8b1013037cef6929f9-300x88.png 300w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/c8856789ec11ab8b1013037cef6929f9-1024x301.png 1024w, https:\/\/admin.acceleratingscience.com\/learning-at-the-bench\/wp-content\/uploads\/sites\/13\/2023\/05\/c8856789ec11ab8b1013037cef6929f9-768x225.png 768w\" sizes=\"auto, (max-width: 1284px) 100vw, 1284px\" \/><p id=\"caption-attachment-14361\" class=\"wp-caption-text\">\u56f33. \u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u5897\u5e45\u66f2\u7dda\u30020\u6642\u9593\uff08\u8d64\u7dda\uff09\u306814\u65e5\u9593\uff08\u9752\u7dda\uff09\u653e\u7f6e\u3057\u305fRNA\u306e\u7d50\u679c<\/p><\/div>\n<p>\u0394C<sub>T<\/sub>\u5024\u304b\u3089\u5927\u307e\u304b\u306a\u5206\u89e3\u5ea6\u3092\u8a08\u7b97\u3057\u3066\u307f\u308b\u3068\u300118S rRNA\u3067\u306f2<sup>0.34<\/sup> \u22521.27\u500d\u306e\u5dee\uff08\u7d0479%\u306b\u6e1b\u5c11\uff09\u3001Beta catenin\u3067\u306f2<sup>0.20<\/sup> \u22521.15\u500d\u306e\u5dee\uff08\u7d0487%\u306b\u6e1b\u5c11\uff09\u3068\u3044\u3046\u5024\u304c\u5f97\u3089\u308c\u307e\u3057\u305f\u3002\u5dee\u304c\u975e\u5e38\u306b\u5c0f\u3055\u3044\u305f\u3081\u3042\u304f\u307e\u3067\u3082\u53c2\u8003\u7a0b\u5ea6\u306e\u5024\u3067\u3059\u304c\u3001\u5168\u4f53\u306e1\uff5e2\u5272\u304c\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u3067\u3082\u691c\u51fa\u3067\u304d\u306a\u3044\u307b\u3069\u306b\u5206\u89e3\u3057\u305f\u3001\u3068\u3044\u3046\u89e3\u91c8\u3082\u3067\u304d\u305d\u3046\u3067\u3059\u3002<\/p>\n<h2>\u307e\u3068\u3081<\/h2>\n<ul>\n<li>0\u6642\u9593\u304b\u308914\u65e5\u307e\u3067\u3001\u7cbe\u88fd\u3057\u305fRNA\u3092\u5e38\u6e29\u3067\u653e\u7f6e\u3057\u3066\u5206\u89e3\u5ea6\u3092\u8a55\u4fa1\u3057\u307e\u3057\u305f<\/li>\n<li>\u653e\u7f6e1\u65e5\u5f8c\u306b\u306f\u3084\u3084\u5206\u89e3\u304c\u898b\u3089\u308c\u59cb\u3081\u30017\u65e5\u5f8c\u306b\u306fRIN\u5024\u304c5\u3092\u4e0b\u56de\u308b\u304f\u3089\u3044\u5206\u89e3\u304c\u9032\u307f\u307e\u3057\u305f<\/li>\n<li>\u653e\u7f6e2\u30013\u65e5\u304f\u3089\u3044\u3067\u3042\u308c\u3070RIN\u5024\u306f8\u7a0b\u5ea6\u3067\u3001\u4e00\u822c\u7684\u306a\u5b9f\u9a13\u306b\u306f\u8010\u3048\u3046\u308b\u7a0b\u5ea6\u306e\u54c1\u8cea\u3067\u3057\u305f<\/li>\n<li>\u30ea\u30a2\u30eb\u30bf\u30a4\u30e0PCR\u306e\u7d50\u679c\u3067\u306f\u30010\u6642\u9593\u306814\u65e5\u653e\u7f6e\u306e\u30b5\u30f3\u30d7\u30eb\u9593\u3067\u3001\u308f\u305a\u304b\u306bC<sub>T<\/sub>\u5024\u306e\u5897\u52a0\u304c\u898b\u3089\u308c\u307e\u3057\u305f\u304c\u3001\u0394C<sub>T<\/sub>\u5024\u306f\u5c0f\u3055\u304f\u3001\u5f71\u97ff\u306f\u9650\u5b9a\u7684\u3067\u3057\u305f<\/li>\n<\/ul>\n<p>\u3044\u304b\u304c\u3067\u3057\u305f\u3067\u3057\u3087\u3046\u304b\u3002<br 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